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https://www.readbyqxmd.com/read/28494340/simple-and-rapid-quantification-of-vancomycin-in-serum-urine-and-peritoneal-pleural-effusion-via-uhplc-ms-ms-applicable-to-personalized-antibiotic-dosing-research
#1
Lenka Javorska, Lenka Kujovska Krcmova, Petr Solich, Milan Kaska
Management of the therapy of life-threatening bacterial infection is extremely based on an optimal antibiotic treatment. Achieving the correct vancomycin dosage in blood and target tissues can be complicated in special situations, e.g., where large fluid sequestration and/or acute renal failure occur. A UHPLC-MS/MS method operating in electrospray (ESI) positive ion mode was applied for the determination of vancomycin in serum, urine and peritoneal/pleural effusion. Sample pretreatment was composed of dilution and simple protein precipitation where only a small volume (50μL) of serum, urine or peritoneal/pleural effusion was required...
May 2, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28494155/principles-for-tuning-hydrophobic-ligand-receptor-binding-kinetics
#2
Gregor Weiß, Piotr Setny, Joachim Dzubiella
We investigate how to tune the rate of hydrophobic ligand-receptor association due to the role of solvent in adjustable receptor pockets by explicit-water molecular dynamics (MD) simulations. Our model considers the binding of a spherical ligand (key/guest) to a concave surface recess in a non-polar wall as receptor (lock/host). We systematically modify the receptor's physicochemical properties in terms of geometry and dispersion attraction which, in turn, alter the water occupancy and fluctuations within the pocket...
May 11, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28489385/nano-scaled-amorphous-tio2-hollow-spheres-ticl4-liquid-droplets-based-hydrolysis-fabrication-and-strong-hollow-structure-enhanced-sers-effects
#3
Haoming Bao, Hongwen Zhang, Guangqiang Liu, Yue Li, Weiping Cai
A very simple route is developed to fast fabricate nano-sized amorphous titanium dioxide (TiO2) hollow spheres just via dropping the pure four titanium chloride (TiCl4) liquid droplets into deionized water around room temperature. The TiO2 hollow spheres (THPs), with around 80 nm in mean diameter, can be formed within few seconds after dropping TiCl4 droplets into water. The shell layers of the obtained THPs are amorphous and porous in structure with porosity of 58% - 80% and show linear increase in the thickness with the THPs' size...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28481520/janus-graphene-oxide-sponges-for-high-purity-fast-separation-of-both-water-in-oil-and-oil-in-water-emulsions
#4
Jongju Yun, Fakhre Alam Khan, Seunghyun Baik
Membrane separation of oil and water with high purity and high permeability is of great interest in environmental and industrial processes. However, membranes with fixed wettability can separate only one type of surfactant-stabilized emulsion (water-in-oil or oil-in-water). Here, we report on Janus graphene oxide (J-GO) sponges for high purity and high permeability separation of both water-in-oil and oil-in-water emulsions. Millimeter-scale reduced GO sponges with a controlled pore size (11.2 or 94.1 μm) are synthesized by freeze drying, and the wettability is further controlled by fluorine (hydrophobic/oleophilic in air) or oxygen (hydrophilic/oleophilic in air) functionalization...
May 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28458214/seasonal-variability-of-estuarine-dynamics-due-to-freshwater-discharge-and-its-influence-on-biological-productivity-in-yeongsan-river-estuary-korea
#5
Yong Hoon Kim, Seongjin Hong, Yong Sik Song, Hyojin Lee, Hae-Cheol Kim, Jongseong Ryu, Jinsoon Park, Bong-Oh Kwon, Chang-Hee Lee, Jong Seong Khim
In order to evaluate water quality and biological productivity, observation data sets were collected and analyzed in Yeongsan River Estuary, Korea. We also set up a numerical model to resolve hydrodynamics and fate of water quality variables in the system. Results show that most of nutrients loading are trapped in the lake and higher concentrations of nutrients and organic matters (OM) are present only inside of the artificial sea dike. There exist episodial discharges at the dam, which coincide mostly with rainfall events during summer monsoon periods...
April 22, 2017: Chemosphere
https://www.readbyqxmd.com/read/28456463/regime-shifts-and-ecological-catastrophes-in-a-model-of-plankton-oxygen-dynamics-under-the-climate-change
#6
Sergei Petrovskii, Yadigar Sekerci, Ezio Venturino
It is estimated that more than a half of the total atmospheric oxygen is produced in the oceans due to the photosynthetic activity of phytoplankton. Any significant decrease in the net oxygen production by phytoplankton is therefore likely to result in the depletion of atmospheric oxygen and in a global mass mortality of animals and humans. In its turn, the rate of oxygen production is known to depend on water temperature and hence can be affected by the global warming. We address this problem theoretically by considering a model of a coupled plankton-oxygen dynamics where the rate of oxygen production slowly changes with time to account for the ocean warming...
April 26, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28449274/a-dna-barcode-library-for-germany-s-mayflies-stoneflies-and-caddisflies-ephemeroptera-plecoptera-trichoptera
#7
Jérôme Morinière, Lars Hendrich, Michael Balke, Arne J Beermann, Tobias König, Monika Hess, Stefan Koch, Reinhard Müller, Florian Leese, Paul D N Hebert, Axel Hausmann, Christoph F Schubart, Gerhard Haszprunar
Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. However, a comprehensive DNA barcode library based upon taxonomically well-curated specimens can overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient, and reliable DNA-based identifications and assessments of ecological status...
April 27, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28440630/hydrolytically-stable-luminescent-cationic-metal-organic-framework-for-highly-sensitive-and-selective-sensing-of-chromate-anions-in-natural-water-systems
#8
Wei Liu, Yanlong Wang, Zhuanling Bai, Yuxiang Li, Yaxing Wang, Lanhua Chen, Lin Xu, Juan Diwu, Zhifang Chai, Shuao Wang
Effective detection of chromate anions in aqueous solution is highly desirable because of their high solubility, environmental mobility, carcinogenicity, and bioaccumulation effect. A new strategy for precise detection of chromate anions in the presence of a large excess of other anions, such as Cl(-), NO3(-), and HCO3(-), in drinking water and natural water systems remains a challenge. Herein, a hydrolytically stable cationic luminescent europium(III)-based metal organic framework (MOF), 1, was successfully synthesized and investigated as a luminescent sensor that exhibits instant and selective luminescence quenching properties toward chromate ions in aqueous solutions...
May 5, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28436674/general-dialdehyde-click-chemistry-for-amine-bioconjugation
#9
Sina Elahipanah, Paul J O'Brien, Dmitry Rogozhnikov, Muhammad N Yousaf
The development of methods for conjugating a range of molecules to primary amine functional groups has revolutionized the fields of chemistry, biology, and material science. The primary amine is a key functional group and one of the most important nucleophiles and bases used in all of synthetic chemistry. Therefore, tremendous interest in the synthesis of molecules containing primary amines and strategies to devise chemical reactions to react with primary amines has been at the core of chemical research. In particular, primary amines are a ubiquitous functional group found in biological systems as free amino acids, as key side chain lysines in proteins, and in signaling molecules and metabolites and are also present in many natural product classes...
April 24, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28428822/in-muro-deacetylation-of-xylan-affects-lignin-properties-and-improves-saccharification-of-aspen-wood
#10
Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J Jönsson, Ewa J Mellerowicz
BACKGROUND: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28425700/reaction-of-the-tricyanoborate-dianion-b-cn-3-2-with-hgcl2
#11
Eduard Bernhardt, Vera Bernhardt-Pitchougina, Carsten Jenne
The very reactive [B(CN)3](2-) dianion has a strongly nucleophilic boron atom and can be used for the synthesis of tricyanoborates, which otherwise are difficult to access. Herein the reaction of this anion with HgCl2 is reported. The main product is the anionic mercury complex [Hg(B(CN)3)2](2-). Heteronuclear NMR spectroscopic experiments shows that the reaction proceeds via the intermediate [ClHgB(CN)3](2-). Even though [Hg(B(CN)3)2](2-) is the main product, it is difficult to obtain it in pure form, because it slowly decomposes in the presence of water and air to [(NC)HgB(CN)3](-)...
April 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28422569/preparation-of-a-novel-antibacterial-chitosan-poly-ethylene-glycol-cryogel-silver-nanoparticles-composites
#12
Xueqing Zou, Pengpeng Deng, Changjiang Zhou, Yulin Hou, Rongsheng Chen, Liang Feng, Liqiong Liao
Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization. Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction...
April 19, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28421317/effect-of-oral-carbohydrate-with-amino-acid-solution-on-serum-oxidative-anti-oxidative-status-in-healthy-volunteers
#13
Yasuo M Tsutsumi, Takuro Oyama, Nami Kakuta, Yousuke T Horikawa, Katsuyoshi Kume, Yoko Sakai, Rie Tsutsumi, Katsuya Tanaka
The aim of this work was to investigate the effect of oral carbohydrate with amino acid [oral nutritional supplement (ONS)] solution on oxidative stress in healthy persons. Fourteen healthy volunteers were segregated into control and ONS groups. Volunteers in the ONS group ingested 250 ml of Arginaid Water (Nestle Japan, Tokyo, Japan) in the evening before the experiment and at 7:00 am on the day of the experiment. Volunteers in the control group fasted after dinner and drank only water until 7:00 am on the day of the experiment...
April 18, 2017: Journal of Anesthesia
https://www.readbyqxmd.com/read/28406081/ecotoxicological-analysis-of-acrylamide-using-a-microalga-as-an-indicator-organism
#14
Bárbara Werle Nunes Keila Fernanda Hedlund Maria Angelica Oliveira And Elvis Carissimi
Aquatic ecosystems are very prone to contamination. The fast and accurate assessment of the toxicity of the water is animportant tool for environmental safety. The aim of this study was to assess the possible toxic effects of acrylamide on physiological parameters of the ChlorophyceaeandDesmodesmus quadricauda. Cells in exponential growth phase were exposed for 72 hours to different concentrations of acrylamide. Light microscopy analysis documented cell density, number of cells per coenobia, cell dimensions, and biovolume; chlorophyll "a" concentration was also determined...
April 12, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28400101/overcoming-the-exacerbating-effects-of-ranitidine-on-nsaid-induced-small-intestinal-toxicity-with-quercetin-providing-a-complete-gi-solution
#15
Devendra Pratap Singh, Swapnil P Borse, Manish Nivsarkar
There is a need to find/discover novel leads to treat complex and/or multi-factorial-pathogenic disease(s) like Nonsteroidal anti-inflammatory drugs (NSAID)-induced gastroenteropathy or gastrointestinal (GI) toxicity as it has emerged as an important medical and socioeconomic problem. There is no approved therapeutic strategy to prevent NSAID-induced small intestinal toxicity and highly effective gastro-protective drugs such as ranitidine hydrochloride (RAN) exacerbate it. In this purview, the multi target drug discovery approach (MTDD), combination approach and hit to lead strategies based on the foundation of ethnopharmacology and/or reverse pharmacology holds strong potential...
April 8, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28395829/thermal-analysis-methods-for-the-rapid-identification-and-authentication-of-swiftlet-aerodramus-fuciphagus-edible-bird-s-nest-a-mucin-glycoprotein
#16
Eric K S Shim, Gleen F Chandra, Soo-Y Lee
Edible bird's nest of the swiftlet Aerodramus fuciphagus, is an unusual dried mucin glycoprotein, which has been used, particularly by Asians, as a premium food and wide spectrum health supplement for centuries. For the first time, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry methods are used for the rapid identification and authentication of edible bird's nest. It is shown that edible bird's nest has a total moisture content of 12.6% which is removed below 200°C, followed by two major decomposition steps on heating under nitrogen gas...
May 2017: Food Research International
https://www.readbyqxmd.com/read/28355155/dispersed-dynamics-of-solvation-in-g-quadruplex-dna-comparison-of-dynamic-stokes-shifts-of-probes-in-parallel-and-antiparallel-quadruplex-structures
#17
Moirangthem Kiran Singh, Him Shweta, Sobhan Sen
G-quadruplex DNA (GqDNA) structures play an important role in many specific cellular functions and are promising anti-tumor targets for small molecules (ligands). Here, we measured the dynamic Stokes shift of a ligand (Hoechst) bound to parallel c-Myc (mPu22) GqDNA over five decades of time from 100 fs to 10 ns, and compared it with the previously reported dynamics of DAPI bound to antiparallel human telomeric (hTelo22) GqDNA (Pal et al 2015 J. Phys. Chem. Lett. 6 1754). Stokes shift data from fluorescence up-conversion and time-correlated single photon counting experiments was combined to cover the broad dynamic range...
September 6, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28351642/inter-laboratory-validation-of-a-thin-film-microextraction-technique-for-determination-of-pesticides-in-surface-water-samples
#18
Hamed Piri-Moghadam, Emanuela Gionfriddo, Angel Rodriguez-Lafuente, Jonathan J Grandy, Heather L Lord, Terry Obal, Janusz Pawliszyn
The primary goal of the present study is the inter-laboratory evaluation of a thin film microextraction (TFME) technique to be used as an alternative approach to liquid-liquid extraction (LLE). Polydimethylsiloxane/divinylbenzene (PDMS/DVB) and PDMS/DVB-carbon mesh supported membranes were used for the extraction of 23 targeted pesticides, while a thermal desorption unit (TDU) was employed to transfer these analytes to a GC/MS instrument for separation and detection. After optimization of the most critical parameters, both membranes were capable of achieving limits of detection (LOD) in the low ng L(-1) range while demonstrating excellent robustness, withstanding up to 100 extractions/desorption cycles...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28350747/short-article-small-intestinal-mucosal-injury-in-patients-taking-chemotherapeutic-agents-for-solid-cancers
#19
Maria P Dore, Giovanni M Pes, Alberto Murino, Bianca Quarta Colosso, Marco Pennazio
OBJECTIVE: Chemotherapy for cancer is a systemic treatment often associated with side effects than can be debilitating and, in some cases, life-threatening. Few data are available on intestinal enterotoxicity. Wireless video capsule endoscopy (VCE) is a noninvasive method of imaging the small intestine. This study presents the results of VCE in patients with solid tumors undergoing antineoplastic regimens with agents, notably for toxicity for the gastrointestinal mucosa (i.e. carboplatin, cyclophosphamide, 5-fluorouracil, methotrexate, and cisplatin)...
May 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28347724/metabolic-effects-of-intermittent-access-to-caloric-or-non-caloric-sweetened-solutions-in-mice-fed-a-high-caloric-diet
#20
Marion Soto, Catherine Chaumontet, Patrick C Even, Dalila Azzout-Marniche, Daniel Tomé, Gilles Fromentin
Human consumption of obesogenic diets and soft drinks, sweetened with different molecules, is increasing worldwide, and increases the risk of metabolic diseases. We hypothesized that the chronic consumption of caloric (sucrose, high-fructose corn syrup (HFCS), maltodextrin) and non-caloric (sucralose) solutions under 2-hour intermittent access, alongside the consumption of a high-fat high-sucrose diet, would result in differential obesity-associated metabolic abnormalities in mice. Male C57BL/6 mice had ad libitum access to an HFHS diet and to water (water control group)...
June 1, 2017: Physiology & Behavior
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