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non-coding RNA AND nasopharyngeal

Wing-Po Chak, Raymond Wai-Ming Lung, Joanna Hung-Man Tong, Sylvia Yat-Yee Chan, Samantha Wei-Man Lun, Sai-Wah Tsao, Kwok-Wai Lo, Ka-Fai To
In our previous whole-transcriptome sequencing analysis, downregulation of a long non-coding RNA, maternally expressed gene 3 (MEG3), was identified in NPC samples. This finding suggests the possible role of MEG3 as a tumor suppressor in this distinctive disease. In the present study, two MEG3 variants, AF119863 (MEG3-AF) and BX247998 (MEG3-BX), were found abundantly expressed in a normal nasopharyngeal epithelial cell line, NP69. Significant downregulation of MEG3-AF was further verified in a panel of NPC samples including xenografts and primary biopsies...
September 6, 2016: Molecular Carcinogenesis
Baoyu He, Weiming Li, Yingfen Wu, Fang Wei, Zhaojian Gong, Hao Bo, Yumin Wang, Xiayu Li, Bo Xiang, Can Guo, Qianjin Liao, Pan Chen, Xuyu Zu, Ming Zhou, Jian Ma, Xiaoling Li, Yong Li, Guiyuan Li, Wei Xiong, Zhaoyang Zeng
Epstein-Barr virus (EBV) infection is causatively related to a variety of human cancers, including nasopharyngeal carcinoma (NPC) and gastric cancer (GC). EBV encodes 44 mature miRNAs, a number of which have been proven to promote carcinogenesis by targeting host genes or self-viral genes. However, in this study, we found that an EBV-encoded microRNA, termed EBV-miR-BART6-3p, inhibited EBV-associated cancer cell migration and invasion including NPC and GC by reversing the epithelial-mesenchymal transition (EMT) process...
2016: Cell Death & Disease
Wen-Feng Hua, Qian Zhong, Tian-Liang Xia, Qi Chen, Mei-Yin Zhang, Ai-Jun Zhou, Zi-Wei Tu, Chen Qu, Man-Zhi Li, Yun-Fei Xia, Hui-Yun Wang, Dan Xie, Francois-Xavier Claret, Er-Wei Song, Mu-Sheng Zeng
Abnormal interaction between non-coding RNAs has been demonstrated to be a common molecular event in various human cancers, but its significance and underlying mechanisms have not been well documented. RNA-binding proteins (RBPs) are key regulators of RNA transcription and post-transcriptional processing. In this study, we found that RNA-binding protein 24 (RBM24) was frequently downregulated in nasopharyngeal carcinoma (NPC). The restoration of RBM24 expression suppressed NPC cellular proliferation, migration and invasion and impeded metastatic colonization in mouse models...
2016: Cell Death & Disease
Peng Song, Lin-Feng Ye, Cen Zhang, Tao Peng, Xu-Hong Zhou
Long non-coding RNA (lncRNA) X inactivate-specific transcript (XIST) has been verified as an oncogenic gene in several human malignant tumors, and its dysregulation was closed associated with tumor initiation, development and progression. Nevertheless, whether the aberrant expression of XIST in human nasopharyngeal carcinoma (NPC) is corrected with malignancy, metastasis or prognosis has not been elaborated. Here, we discovered that XIST was up-regulated in NPC tissues and higher expression of XIST contributed to a markedly poorer survival time...
October 30, 2016: Gene
Li Li, Miao Gu, Bo You, Si Shi, Ying Shan, Lili Bao, Yiwen You
Nasopharyngeal carcinoma (NPC) is one of the most common malignancies of the head and neck. It arises from the nasopharynx epithelium and is associated with high morbidity and mortality. Long non-coding RNA (lncRNA) have been reported to regulate gene interaction and play critical roles in carcinogenesis and progression. LncRNA-ROR, a recently identified lncRNA, has been shown to be involved in initiation, progression and metastasis of several tumors, including hepatocellular carcinoma, breast cancer and glioma...
September 2016: Cancer Science
Massimiliano Bergallo, Chiara Merlino, Davide Montin, Ilaria Galliano, Stefano Gambarino, Katia Mareschi, Franca Fagioli, Paola Montanari, Silvana Martino, Pier-Angelo Tovo
MicroRNAs (miRNAs) are short, single stranded, non-coding RNA molecules. They are produced by many different species and are key regulators of several physiological processes. miRNAs are also encoded by the genomes of multiple virus families, such as herpesvirus family. In particular, miRNAs from Epstein Barr virus were found at high concentrations in different associated pathologies, such as Burkitt's lymphoma, Hodgkin disease, and nasopharyngeal carcinoma. Thanks to their stability, these molecules could possibly serve as biomarkers for EBV-associated diseases...
September 2016: Molecular Biotechnology
Nan Cheng, Guang-Hui Wang
MicroRNAs (miRs) are a class of short and non-coding RNA molecules, which function as either oncogenes or tumor suppressors in the development of various human cancers, including nasopharyngeal carcinoma (NPC). The aim of the present study was to investigate the expression of miR-133b in NPC tissue samples, as compared with adjacent normal tissues, and to examine its roles and underlying mechanisms. Analysis using reverse transcription-quantitative polymerase chain reaction demonstrated that miR-133b was downregulated in NPC tissue samples, as compared with adjacent tissues...
April 2016: Experimental and Therapeutic Medicine
Xudong Li, Yan Lin, Xi Yang, Xiaoguang Wu, Xiaoguang He
The long non-coding RNA (lncRNA) H19 has been recently shown to participate in the progression of cancer, including metastasis. However, the biological role of H19 and the underlying mechanisms mediating its functions in nasopharyngeal carcinoma (NPC) remain unclear. Herein, we found that H19 was overexpressed in NPC tissues and poorly differentiated cell lines. Knockdown of H19 significantly inhibited the invasive ability of NPC cells. Moreover, H19 affected the expression of enhancer of zeste homolog 2 (EZH2), which has also been observed to be up-regulated in NPC and to promote cell invasion...
May 13, 2016: Biochemical and Biophysical Research Communications
Yaoyong Lu, Tao Li, Ganbao Wei, Liangbo Liu, Qinsheng Chen, Lufei Xu, Kunqiang Zhang, Dehao Zeng, Rongwei Liao
Long non-coding RNAs (lncRNAs) play a critical role in cancer progression, including in nasopharyngeal carcinoma (NPC). However, it is still poorly understood whether lncRNA regulates epithelial to mesenchymal transition (EMT) and radioresistance of NPC cells. We found that lncRNA NEAT1 was significantly upregulated in NPC cell lines and tissues. Knockdown of NEAT1 could sensitize NPC cells to radiation in vitro. Further investigation found that NEAT1 regulated radioresistance by modulating EMT phenotype. Furthermore, we found that there was reciprocal repression between NEAT1 and miR-204...
March 28, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Jennifer Y Y Kwan, Pamela Psarianos, Jeff P Bruce, Kenneth W Yip, Fei-Fei Liu
MicroRNAs (miRNAs) are small non-coding RNA molecules that have key regulatory roles in cancer, acting as both oncogenes and tumor suppressors. Due to the potential roles of miRNAs in improving cancer prognostic, predictive, diagnostic and therapeutic approaches, they have become an area of intense research focus in recent years. MiRNAs harbor attractive features allowing for translation to the clinical world, such as relatively simple extraction methods, resistance to molecular degradation, and ability to be quantified...
August 2016: Journal of Radiation Research
Kun Zhuang, Qiang Wu, Chun-Shun Jin, Hui-Jun Yuan, Jin-Zhang Cheng
BACKGROUND: Nasopharyngeal carcinoma (NPC) is a common head and neck cancer with an incidence of 10-30 cases per 100,000 in southern China. Although primary treatment includes radiation therapy, prognosis is still unsatisfactory. OBJECTIVE: In this study, we examined the role of HNF1A-AS in NPC progression in vitro and in vivo. METHODS: Relative levels of long non-coding RNA (LncRNA), HNF1A-AS, were evaluated in tumor tissues from 20 patients with NPC as well as from cultured NPC cell lines...
2016: Cancer Biomarkers: Section A of Disease Markers
Z Zeng, S Fan, X Zhang, S Li, M Zhou, W Xiong, M Tan, W Zhang, G Li
PURPOSE: EBER-1 (a non-coding RNA transcribed by EBV) expression was detected in most of Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) patients. However, the relevance between EBER-1 expression and NPC clinical outcome has not been reported. This study aims to assess the possible correlations of EBER-1 expression and clinical parameters and its potential prognostic predictive ability in NPC patient's outcomes. METHODS: We examined EBER-1 mRNA expression in 301 NPC and 130 non-NPC tissues using in situ hybridization and did statistics...
February 2016: Clinical & Translational Oncology
Hao Bo, Zhaojian Gong, Wenling Zhang, Xiayu Li, Yong Zeng, Qianjin Liao, Pan Chen, Lei Shi, Yu Lian, Yizhou Jing, Ke Tang, Zheng Li, Yanhong Zhou, Ming Zhou, Bo Xiang, Xiaoling Li, Jianbo Yang, Wei Xiong, Guiyuan Li, Zhaoyang Zeng
Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1-AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability...
August 21, 2015: Oncotarget
Jian Jun Chen, Shi Xi Liu, Mian Zhi Chen, Zi Yi Zhao
MicroRNA (miRNA) is a class of non‑coding RNA, which targets mRNAs of interest and suppresses its expression by degradation or translational inhibition. miRNA (miR)‑125a and miR‑125b were previously demonstrated to translationally and transcriptionally inhibit the expression of p53. The observed downregulation of the protein level of p53 in cisplatin‑treated patients with nasopharyngeal carcinoma (NPC) indicates the association between cisplatin resistance, miR‑125a and miR‑125b. In the present study, through the detection of the expression levels of miR‑125a and miR‑125b, a significant upregulation of these miRs was demonstrated in cisplatin‑treated patients with NPC...
September 2015: Molecular Medicine Reports
Runan Zhu, Lei Luo, Linqing Zhao, Jie Deng, Fang Wang, Yu Sun, Qinwei Song, Yaxin Ding, Yuan Qian
Human parechoviruses (HPeVs) belong to the Parechovirus genus of the large and growing family of Picornaviridae with a non-enveloped, single-stranded and positive-sense RNA. An HPeV strain was isolated from the nasopharyngeal aspirate specimen of a 2 months old infant hospitalized with pneumonia in Beijing, China and nominated as BJ-37359 followed the code of the specimen. Strain BJ-37359 was identified as HPeV1 by whole genome sequencing. The full genome of strain BJ-37359 consisted of 7336 nucleotides (nt), excluding a poly (A) tail and contained an ORF of 6537 nt flanked by 5'UTR of 709 nt and 3'UTR of 90 nt...
January 2015: Infection, Genetics and Evolution
Zhaojian Gong, Shanshan Zhang, Zhaoyang Zeng, Hanjiang Wu, Qian Yang, Fang Xiong, Lei Shi, Jianbo Yang, Wenling Zhang, Yanhong Zhou, Yong Zeng, Xiayu Li, Bo Xiang, Shuping Peng, Ming Zhou, Xiaoling Li, Ming Tan, Yong Li, Wei Xiong, Guiyuan Li
Recent studies have revealed that long non-coding RNAs participate in all steps of cancer initiation and progression by regulating protein-coding genes at the epigenetic, transcriptional, and post-transcriptional levels. Long non-coding RNAs are in turn regulated by other genes, forming a complex regulatory network. The regulation networks between the p53 tumor suppressor and these RNAs in nasopharyngeal carcinoma remains unclear. The aims of this study were to investigate the regulatory roles of the TP53 gene in regulating long non-coding RNA expression profiles and to study the function of a TP53-regulated long non-coding RNA (LOC401317) in the nasopharyngeal carcinoma cell line HNE2...
2014: PloS One
Qing-Qing Yang, Yan-Fei Deng
AIMS: Alterations in the expression of several long non-coding RNAs (lncRNAs) have been found in primary nasopharyngeal carcinoma (NPC). However, the effect of lncRNA expression on primary NPC as well as the molecular mechanism of lncRNA remains vague. This study was to identify differentially expressed lncRNAs involved in NPC on a genome-wide scale and predict their potential functions. METHODS AND RESULTS: Using high-throughput microarray with 30,586 lncRNA and 26,109 mRNA probes, 856 lncRNAs and 767 mRNAs were expressed differentially between NPC and chronic nasopharyngitis tissues...
June 2015: Histopathology
Qi-Rui Wang, Hao-Ning Fan, Zhi-Xin Yin, Hong-Bing Cai, Meng Shao, Jian-Xin Diao, Yuan-Liang Liu, Xue-Gang Sun, Li Tong, Qin Fan
OBJECTIVE: To investigate the effect of curcumin (Cur) on radiosensitivity of nasopharyngeal carcinoma cell CNE-2 and its mechanism. METHOD: The effect of curcumin on radiosensitivity was determined by the clone formation assay. The cell survival curve was fitted by Graph prism 6. 0. The changes in cell cycle were analyzed by flow cytometry (FCM). The differential expression of long non-coding RNA was detected by gene chip technology. Part of differentially expressed genes was verified by Real-time PCR...
February 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Wei Gao, Jimmy Yu-Wai Chan, Thian-Sze Wong
BACKGROUND: Recent studies suggested that non-protein-coding genes are implicated in the tumorigenic process of nasopharyngeal carcinoma (NPC). In the present study, we aimed to identify the differentially expressed long noncoding RNA (lncRNA) using data available in the public domain. METHODS: Microarray data set GSE12452 was reannotated with ncFANs. Real-time quantitative PCR was used to quantify and validate the identified lncRNAs in NPC. RESULTS: In primary NPC, upregulation of lnc-C22orf32-1, lnc-AL355149...
2014: BioMed Research International
Mélanie Gressette, Benjamin Vérillaud, Anne-Sophie Jimenez-Pailhès, Hélène Lelièvre, Kwok-Wai Lo, François-Régis Ferrand, Charles-Henry Gattolliat, Anne Jacquet-Bescond, Laurence Kraus-Berthier, Stéphane Depil, Pierre Busson
EBV-related nasopharyngeal carcinomas (NPCs) still raise serious therapeutic problems. The therapeutic potential of the histone-deacetylase (HDAC) inhibitor Abexinostat was investigated using 5 preclinical NPC models including 2 patient-derived xenografts (C15 and C17). The cytotoxicity of Abexinostat used either alone or in combination with cis-platin or irradiation was assessed in vitro by MTT and clonogenic assays using 2 EBV-negative (CNE1 and HONE1) and 3 EBV-positive NPC models (C15, C17 and C666-1). Subsequently, the 3 EBV-positive models were used under the form of xenografts to assess the impact of systemic treatments by Abexinostat or combinations of Abexinostat with cis-platin or irradiation...
2014: PloS One
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