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Zebrafish obesity

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https://www.readbyqxmd.com/read/29289284/exposure-to-endocrine-disrupting-chemicals-perturbs-lipid-metabolism-and-circadian-rhythms
#1
Renate Kopp, Irene Ozáez Martínez, Jessica Legradi, Juliette Legler
A growing body of evidence indicates that exposure to environmental chemicals can contribute to the etiology of obesity by inappropriately stimulating adipogenesis as well as perturbing lipid metabolism and energy balance. One potential mechanism by which chemical exposure can influence lipid metabolism is through disturbance of circadian rhythms, endogenously-driven cycles of roughly 24hr in length that coordinate biochemical, physiological, and behavioral processes in all organisms. Here we show for the first time that exposure to endocrine disrupting compounds (EDCs), including the pesticide tributyltin, two commercial flame retardants, and a UV-filter chemical found in sunscreens, can perturb both circadian clocks and lipid metabolism in vertebrates...
December 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29286302/microrna-27b-depletion-enhances-endotrophic-and-intravascular-lipid-accumulation-and-induces-adipocyte-hyperplasia-in-zebrafish
#2
Chia-Chun Hsu, Chi-Yu Lai, Chiu-Ya Lin, Kun-Yun Yeh, Guor Mour Her
miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, and as a potential therapeutic target for treating atherosclerosis and obesity. However, the impact of miR-27b on lipid levels in vivo remains to be determined. Zebrafish lipids are normally stored as triacylglycerols (TGs) and their main storage sites are visceral, intramuscular, and subcutaneous lipid depots, and not blood vessels and liver. In this study, we applied microRNA-sponge (miR-SP) technology and generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which disrupted endogenous miR-27b activity and induced intravascular lipid accumulation (hyperlipidemia) and the early onset of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH)...
December 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29277029/embryonic-exposure-to-mono-2-ethylhexyl-phthalate-mehp-disrupts-pancreatic-organogenesis-in-zebrafish-danio-rerio
#3
Haydee M Jacobs, Karilyn E Sant, Aviraj Basnet, Larissa M Williams, Jennifer B Moss, Alicia R Timme-Laragy
Mono(2-ethylhexyl) phthalate (MEHP) is the bioactive metabolite of di(2-ethylhexyl) phthalate, a plasticizing agent and persistent environmental contaminant associated with obesity, developmental abnormalities, and oxidative stress. Nrf2 (Nfe2l2) is a transcription factor that regulates cytoprotective genes as part of the adaptive antioxidant response. We previously identified the pancreas as a sensitive target of oxidative stress during embryonic development. The goals of this study were to 1) characterize the effects of MEHP exposure on pancreatic development, and 2) determine whether oxidative stress contributes to MEHP embryotoxicity...
December 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/29274064/metabolic-and-bone-effects-of-high-fat-diet-in-adult-zebrafish
#4
Marta Carnovali, Livio Luzi, Ileana Terruzzi, Giuseppe Banfi, Massimo Mariotti
An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Adult Danio rerio (zebrafish) represents a powerful model to study both metabolic diseases and bone metabolism. The aim of this study was to generate an obese adult zebrafish by high-fat diet and evaluate metabolic and bone tissue effects. Fish blood glucose and insulin levels were found to be altered in high-fat diet fish revealing a failure in β-cells insulin production...
December 22, 2017: Endocrine
https://www.readbyqxmd.com/read/29180630/atf4-overexpression-induces-early-onset-of-hyperlipidaemia-and-hepatic-steatosis-and-enhances-adipogenesis-in-zebrafish
#5
Kun-Yun Yeh, Chi-Yu Lai, Chiu-Ya Lin, Chia-Chun Hsu, Chung-Ping Lo, Guor Mour Her
Activating transcription factor 4 (ATF4) is constitutively expressed in a variety of tissues, and regulates several pathological features associated with metabolic diseases such as non-alcoholic fatty liver diseases (NAFLD) and obesity. However, the role of ATF4 in animal model systems is poorly understood. To investigate ATF4 functions in zebrafish, we conditionally expressed ATF4 proteins, using a Tet-off transgenic system. We observed early-onset hyperlipidaemia and liver steatosis in ATF4 transgenic zebrafish (ATs) without doxycycline treatment (ATs - Dox)...
November 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29093028/genome-wide-meta-analysis-unravels-interactions-between-magnesium-homeostasis-and-metabolic-phenotypes
#6
Tanguy Corre, Francisco J Arjona, Caroline Hayward, Sonia Youhanna, Jeroen H F de Baaij, Hendrica Belge, Nadine Nägele, Huguette Debaix, Maxime G Blanchard, Michela Traglia, Sarah E Harris, Sheila Ulivi, Rico Rueedi, David Lamparter, Aurélien Macé, Cinzia Sala, Stefania Lenarduzzi, Belen Ponte, Menno Pruijm, Daniel Ackermann, Georg Ehret, Daniela Baptista, Ozren Polasek, Igor Rudan, Toby W Hurd, Nicholas D Hastie, Veronique Vitart, Geràrd Waeber, Zoltán Kutalik, Sven Bergmann, Rosa Vargas-Poussou, Martin Konrad, Paolo Gasparini, Ian J Deary, John M Starr, Daniela Toniolo, Peter Vollenweider, Joost G J Hoenderop, René J M Bindels, Murielle Bochud, Olivier Devuyst
Magnesium (Mg2+) homeostasis is critical for metabolism. However, the genetic determinants of the renal handling of Mg2+, which is crucial for Mg2+ homeostasis, and the potential influence on metabolic traits in the general population are unknown. We obtained plasma and urine parameters from 9099 individuals from seven cohorts, and conducted a genome-wide meta-analysis of Mg2+ homeostasis. We identified two loci associated with urinary magnesium (uMg), rs3824347 (P=4.4×10-13) near TRPM6, which encodes an epithelial Mg2+ channel, and rs35929 (P=2...
November 1, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28953247/anti-adipogenic-effects-on-3t3-l1-cells-and-zebrafish-by-tanshinone-iia
#7
Yu-Kyoung Park, Brice Wilfried Obiang-Obounou, Jinho Lee, Tae-Yun Lee, Myung-Ae Bae, Kyu-Seok Hwang, Kyung-Bok Lee, Jong-Soon Choi, Byeong-Churl Jang
Tanshinone IIA is a diterpene quinone isolated from the roots of Salviamiltiorrhiza bunge that has traditionally been used in China for the treatment of cardiovascular and cerebrovascular disorders. Although there is recent evidence showing that tanshinone IIA has an anti-obesity effect, its underlying mechanism of anti-obesity effect is poorly understood. Here, we investigated the effect of tanshinone IIA on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Notably, tanshinone IIA at 10 μM concentration greatly reduced lipid accumulation and triglyceride (TG) contents during 3T3-L1 preadipocyte differentiation, suggesting its anti-adipogenic effect...
September 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28934389/the-16p11-2-homologs-fam57ba-and-doc2a-generate-certain-brain-and-body-phenotypes
#8
Jasmine M McCammon, Alicia Blaker-Lee, Xiao Chen, Hazel Sive
Deletion of the 16p11.2 CNV affects 25 core genes and is associated with multiple symptoms affecting brain and body, including seizures, hyperactivity, macrocephaly, and obesity. Available data suggest that most symptoms are controlled by haploinsufficiency of two or more 16p11.2 genes. To identify interacting 16p11.2 genes, we used a pairwise partial loss of function antisense screen for embryonic brain morphology, using the accessible zebrafish model. fam57ba, encoding a ceramide synthase, was identified as interacting with the doc2a gene, encoding a calcium-sensitive exocytosis regulator, a genetic interaction not previously described...
October 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28859103/myt1l-mutations-cause-intellectual-disability-and-variable-obesity-by-dysregulating-gene-expression-and-development-of-the-neuroendocrine-hypothalamus
#9
Patricia Blanchet, Martina Bebin, Shaam Bruet, Gregory M Cooper, Michelle L Thompson, Benedicte Duban-Bedu, Benedicte Gerard, Amelie Piton, Sylvie Suckno, Charu Deshpande, Virginia Clowes, Julie Vogt, Peter Turnpenny, Michael P Williamson, Yves Alembik, Eric Glasgow, Alisdair McNeill
Deletions at chromosome 2p25.3 are associated with a syndrome consisting of intellectual disability and obesity. The smallest region of overlap for deletions at 2p25.3 contains PXDN and MYT1L. MYT1L is expressed only within the brain in humans. We hypothesized that single nucleotide variants (SNVs) in MYT1L would cause a phenotype resembling deletion at 2p25.3. To examine this we sought MYT1L SNVs in exome sequencing data from 4, 296 parent-child trios. Further variants were identified through a genematcher-facilitated collaboration...
August 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28856758/gonadal-soma-controls-ovarian-follicle-proliferation-through-gsdf-in-zebrafish
#10
Yi-Lin Yan, Thomas Desvignes, Ruth Bremiller, Catherine Wilson, Danielle Dillon, Samantha High, Bruce Draper, Charles Loren Buck, John Postlethwait
BACKGROUND: Aberrant signaling between germ cells and somatic cells can lead to reproductive disease and depends on diffusible signals, including transforming growth factor-beta (TGFB) -family proteins. The TGFB-family protein Gsdf (gonadal soma derived factor) controls sex determination in some fish and is a candidate for mediating germ cell/soma signaling. RESULTS: Zebrafish expressed gsdf in somatic cells of bipotential gonads and expression continued in ovarian granulosa cells and testicular Sertoli cells...
August 30, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28842785/effects-of-overfeeding-and-high-fat-diet-on-cardiosomatic-parameters-and-cardiac-structures-in-young-and-adult-zebrafish
#11
Rafael Vargas, Isabel Cristina Vásquez
Obesity is a complex global health problem because it is a risk factor for multiple chronic pathologies such as cardiovascular, endocrine, metabolic, and neoplastic diseases. It is considered a multicausal disease, and one of the determining factors is nutritional imbalances, which include high-fat diets. In this paper, we use the zebrafish model to assess the impact of overfeeding and a high-fat diet in somatic and cardiac parameters in young and adult zebrafish. The results show that fish receiving a high-fat diet showed greater weight gain compared to fish receiving a standard fat diet...
December 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28717234/dietary-lipid-content-reorganizes-gut-microbiota-and-probiotic-l-rhamnosus-attenuates-obesity-and-enhances-catabolic-hormonal-milieu-in-zebrafish
#12
Silvia Falcinelli, Ana Rodiles, Azadeh Hatef, Simona Picchietti, Lina Cossignani, Daniel L Merrifield, Suraj Unniappan, Oliana Carnevali
In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28686680/resveratrol-ameliorates-diet-induced-dysregulation-of-lipid-metabolism-in-zebrafish-danio-rerio
#13
Gai Ran, Li Ying, Lin Li, Qiaoqiao Yan, Weijie Yi, Chenjiang Ying, Hongmei Wu, Xiaolei Ye
Defective lipid metabolism is associated with increased risk of various chronic diseases, such as obesity, cardiovascular diseases, and diabetes. Resveratrol (RSV), a natural polyphenol, has been shown the potential of ameliorating disregulations of lipid metabolism. The objective of this study was to investigate the effects of feed intake and RSV on lipid metabolism in zebrafish (Danio rerio). The adult males were randomly allocated to 6 groups: control (Con, 8 mg cysts/fish/day), control with 20 μmol/L RSV (Con+RSV), calorie restriction (CR, 5 mg cysts/fish/day), calorie restriction with RSV (CR+RSV), overfeed (OF, 60 mg cysts/fish/day), and overfeed with RSV (OF+RSV) groups...
2017: PloS One
https://www.readbyqxmd.com/read/28588550/the-role-of-peroxisome-proliferator-activated-receptor-gamma-pparg-in-adipogenesis-applying-knowledge-from-the-fish-aquaculture-industry-to-biomedical-research
#14
REVIEW
Rebecca Wafer, Panna Tandon, James E N Minchin
The tropical freshwater zebrafish has recently emerged as a valuable model organism for the study of adipose tissue biology and obesity-related disease. The strengths of the zebrafish model system are its wealth of genetic mutants, transgenic tools, and amenability to high-resolution imaging of cell dynamics within live animals. However, zebrafish adipose research is at a nascent stage and many gaps exist in our understanding of zebrafish adipose physiology and metabolism. By contrast, adipose research within other, closely related, teleost species has a rich and extensive history, owing to the economic importance of these fish as a food source...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28575319/translational-research-in-the-nonhuman-primate-model-of-tuberculosis
#15
Taylor W Foreman, Smriti Mehra, Andrew A Lackner, Deepak Kaushal
Infection with Mycobacterium tuberculosis predominantly establishes subclinical latent infection over the lifetime of an individual, with a fraction of infected individuals rapidly progressing to active disease. The immune control in latent infection can be perturbed by comorbidities such as diabetes mellitus, obesity, smoking, and coinfection with helminthes or HIV. Modeling the varying aspects of natural infection remains incomplete when using zebrafish and mice. However, the nonhuman primate model of tuberculosis offers a unique and accurate model to investigate host responses to infection, test novel therapeutics, and thoroughly assess preclinical vaccine candidates...
May 31, 2017: ILAR Journal
https://www.readbyqxmd.com/read/28514663/fatty-acid-oxidation-in-zebrafish-adipose-tissue-is-promoted-by-1%C3%AE-25-oh-2d3
#16
Xuyan Peng, Guohui Shang, Wenqing Wang, Xiaowen Chen, Qiyong Lou, Gang Zhai, Dongliang Li, Zhenyu Du, Yali Ye, Xia Jin, Jiangyan He, Yi Zhang, Zhan Yin
1α,25(OH)2D3 (vitamin D3) is crucial for mineral homeostasis in mammals, but the precise effects of 1α,25(OH)2D3 in adipose tissue remain to be clarified in vivo. The initial 25-hydroxylation is catalyzed by liver microsomal cytochrome P450 2R1 (CYP2R1), which is conserved in vertebrates. To probe the physiological function(s) of 1α,25(OH)2D3 in teleosts, we generated two independent cyp2r1-deficient zebrafish lines. These mutants exhibit retarded growth and increased obesity, especially in the visceral adipose tissue (VAT)...
May 16, 2017: Cell Reports
https://www.readbyqxmd.com/read/28509846/a-new-ergosterol-analog-a-new-bis-anthraquinone-and-anti-obesity-activity-of-anthraquinones-from-the-marine-sponge-associated-fungus-talaromyces-stipitatus-kufa-0207
#17
Jidapa Noinart, Suradet Buttachon, Tida Dethoup, Luís Gales, José A Pereira, Ralph Urbatzka, Sara Freitas, Michael Lee, Artur M S Silva, Madalena M M Pinto, Vítor Vasconcelos, Anake Kijjoa
A new ergosterol analog, talarosterone (1) and a new bis-anthraquinone derivative (3) were isolated, together with ten known compounds including palmitic acid, ergosta-4,6,8(14),22-tetraen-3-one, ergosterol-5,8-endoperoxide, cyathisterone (2), emodin (4a), questinol (4b), citreorosein (4c), fallacinol (4d), rheoemodin (4e) and secalonic acid A (5), from the ethyl acetate extract of the culture of the marine sponge-associated fungus Talaromyces stipitatus KUFA 0207. The structures of the new compounds were established based on extensive 1D and 2D spectral analysis, and in the case of talarosterone (1), the absolute configurations of its stereogenic carbons were determined by X-ray crystallographic analysis...
May 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28469250/development-of-a-novel-zebrafish-model-for-type-2-diabetes-mellitus
#18
Liqing Zang, Yasuhito Shimada, Norihiro Nishimura
Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using an automated feeding system. We monitored the fasting blood glucose levels in the normal-fed group (one feed/day) and overfed group (six feeds/day) over an 8-week period. The fasting blood glucose level was significantly increased in DIO zebrafish compared with that of normal-fed zebrafish...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28441764/altered-adipogenesis-in-zebrafish-larvae-following-high-fat-diet-and-chemical-exposure-is-visualised-by-stimulated-raman-scattering-microscopy
#19
Marjo J den Broeder, Miriam J B Moester, Jorke H Kamstra, Peter H Cenijn, Valentina Davidoiu, Leonie M Kamminga, Freek Ariese, Johannes F de Boer, Juliette Legler
Early life stage exposure to environmental chemicals may play a role in obesity by altering adipogenesis; however, robust in vivo methods to quantify these effects are lacking. The goal of this study was to analyze the effects of developmental exposure to chemicals on adipogenesis in the zebrafish (Danio rerio). We used label-free Stimulated Raman Scattering (SRS) microscopy for the first time to image zebrafish adipogenesis at 15 days post fertilization (dpf) and compared standard feed conditions (StF) to a high fat diet (HFD) or high glucose diet (HGD)...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28337027/obesity-induced-decreases-in-muscle-performance-are-not-reversed-by-weight-loss
#20
F Seebacher, J Tallis, K McShea, R S James
BACKGROUND/OBJECTIVES: Obesity can affect muscle phenotypes, and may thereby constrain movement and energy expenditure. Weight loss is a common and intuitive intervention for obesity, but it is not known whether the effects of obesity on muscle function are reversible by weight loss. Here we tested whether obesity-induced changes in muscle metabolic and contractile phenotypes are reversible by weight loss. SUBJECTS/METHODS: We used zebrafish (Danio rerio) in a factorial design to compare energy metabolism, locomotor capacity, muscle isometric force and work-loop power output, and myosin heavy chain (MHC) composition between lean fish, diet-induced obese fish, and fish that were obese and then returned to lean body mass following diet restriction...
August 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
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