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Zebrafish fitness

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https://www.readbyqxmd.com/read/29672321/metabolic-plasticity-enables-circadian-adaptation-to-acute-hypoxia-in-zebrafish-cells
#1
Adolf M Sandbichler, Bianca Jansen, Bettina A Peer, Monika Paulitsch, Bernd Pelster, Margit Egg
BACKGROUND/AIMS: Reduced oxygen availability, hypoxia, is frequently encountered by organisms, tissues and cells, in aquatic environments as well as in high altitude or under pathological conditions such as infarct, stroke or cancer. The hypoxic signaling pathway was found to be mutually intertwined with circadian timekeeping in vertebrates and, as reported recently, also in mammals. However, the impact of hypoxia on intracellular metabolic oscillations is still unknown. METHODS: For determination of metabolites we used Multilabel Reader based fluorescence and luminescence assays, circadian levels of Hypoxia Inducible Factor 1 alpha and oxidized peroxiredoxins were semi quantified by Western blotting and ratiometric quantification of cytosolic and mitochondrial H2O2 was achieved with stable transfections of a redox sensitive green fluorescent protein sensor into zebrafish fibroblasts...
April 16, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29594069/-tetrahymena-thermophila-predation-enhances-environmental-adaptation-of-the-carp-pathogenic-strain-aeromonas-hydrophila-nj-35
#2
Jin Liu, Yuhao Dong, Nannan Wang, Shougang Li, Yuanyuan Yang, Yao Wang, Furqan Awan, Chengping Lu, Yongjie Liu
Persistence of Aeromonas hydrophila in aquatic environments is the principle cause of fish hemorrhagic septicemia. Protistan predation has been considered to be a strong driving force for the evolution of bacterial defense strategies. In this study, we investigated the adaptive traits of A. hydrophila NJ-35, a carp pathogenic strain, in response to Tetrahymena thermophila predation. After subculturing with Tetrahymena , over 70% of A. hydrophila colonies were small colony variants (SCVs). The SCVs displayed enhanced biofilm formation, adhesion, fitness, and resistance to bacteriophage infection and oxidative stress as compared to the non- Tetrahymena- exposed strains...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29476831/adhesion-and-volume-constraints-via-nonlocal-interactions-determine-cell-organisation-and-migration-profiles
#3
José Antonio Carrillo, Annachiara Colombi, Marco Scianna
The description of the cell spatial pattern and characteristic distances is fundamental in a wide range of physio-pathological biological phenomena, from morphogenesis to cancer growth. Discrete particle models are widely used in this field, since they are focused on the cell-level of abstraction and are able to preserve the identity of single individuals reproducing their behavior. In particular, a fundamental role in determining the usefulness and the realism of a particle mathematical approach is played by the choice of the intercellular pairwise interaction kernel and by the estimate of its parameters...
February 21, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29434264/urban-stormwater-runoff-negatively-impacts-lateral-line-development-in-larval-zebrafish-and-salmon-embryos
#4
Alexander Young, Valentin Kochenkov, Jenifer K McIntyre, John D Stark, Allison B Coffin
After a storm, water often runs off of impervious urban surfaces directly into aquatic ecosystems. This stormwater runoff is a cocktail of toxicants that have serious effects on the ecological integrity of aquatic habitats. Zebrafish that develop in stormwater runoff suffer from cardiovascular toxicity and impaired growth, but the effects of stormwater on fish sensory systems are not understood. Our study investigated the effect of stormwater on hair cells of the lateral line in larval zebrafish and coho salmon...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29431558/toxicity-assessment-of-biological-suspensions-using-the-dielectric-impedance-spectroscopy-technique
#5
S Muñoz, J L Sebastián, P Antoranz, J P García-Cambero, A Sanchis-Otero
PURPOSE: This paper studies the variation of the electromagnetic parameters of a suspension of zebrafish (Danio rerio) embryos to assess its potential applications to toxicological and biomedical research areas. MATERIALS AND METHODS: For this purpose, the dielectric impedance spectroscopy technique is applied to a modified coaxial line enclosing the biological suspension to be characterized in the frequency range from 100 kHz to 100 MHz. The electrical parameters of the suspension under test were obtained by fitting the impedance spectra to the resulted from the simulation of the test fixture using finite elements (FE)...
February 12, 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29386525/nonlinear-mixed-modelling-discriminates-the-effect-of-chemicals-and-their-mixtures-on-zebrafish-behavior
#6
Patrick T Gauthier, Mathilakath M Vijayan
Zebrafish (Danio rerio) early-life stage behavior has the potential for high-throughput screening of neurotoxic environmental contaminants. However, zebrafish embryo and larval behavioral assessments typically utilize linear analyses of mean activity that may not capture the complexity of the behavioral response. Here we tested the hypothesis that nonlinear mixed-modelling of zebrafish embryo and larval behavior provides a better assessment of the impact of chemicals and their mixtures. We demonstrate that zebrafish embryo photomotor responses (PMRs) and larval light/dark locomotor activities can be fit by asymmetric Lorentzian and Ricker-beta functions, respectively, which estimate the magnitude of activity (e...
January 31, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29367392/evidence-that-fertility-trades-off-with-early-offspring-fitness-as-males-age
#7
Sheri L Johnson, Sylvia Zellhuber-McMillan, Joanne Gillum, Jessica Dunleavy, Jonathan P Evans, Shinichi Nakagawa, Neil J Gemmell
Models of ageing predict that sperm function and fertility should decline with age as sperm are exposed to free radical damage and mutation accumulation. However, theory also suggests that mating with older males should be beneficial for females because survival to old age is a demonstration of a male's high genetic and/or phenotypic quality. Consequently, declines in sperm fitness may be offset by indirect fitness benefits exhibited in offspring. While numerous studies have investigated age-based declines in male fertility, none has taken the integrated approach of studying age-based effects on both male fertility and offspring fitness...
January 31, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29339520/targeted-knockout-of-a-chemokine-like-gene-increases-anxiety-and-fear-responses
#8
Jung-Hwa Choi, Yun-Mi Jeong, Sujin Kim, Boyoung Lee, Krishan Ariyasiri, Hyun-Taek Kim, Seung-Hyun Jung, Kyu-Seok Hwang, Tae-Ik Choi, Chul O Park, Won-Ki Huh, Matthias Carl, Jill A Rosenfeld, Salmo Raskin, Alan Ma, Jozef Gecz, Hyung-Goo Kim, Jin-Soo Kim, Ho-Chul Shin, Doo-Sang Park, Robert Gerlai, Bradley B Jamieson, Joon S Kim, Karl J Iremonger, Sang H Lee, Hee-Sup Shin, Cheol-Hee Kim
Emotional responses, such as fear and anxiety, are fundamentally important behavioral phenomena with strong fitness components in most animal species. Anxiety-related disorders continue to represent a major unmet medical need in our society, mostly because we still do not fully understand the mechanisms of these diseases. Animal models may speed up discovery of these mechanisms. The zebrafish is a highly promising model organism in this field. Here, we report the identification of a chemokine-like gene family, samdori ( sam ), and present functional characterization of one of its members, sam2 We show exclusive mRNA expression of s am2 in the CNS, predominantly in the dorsal habenula, telencephalon, and hypothalamus...
January 30, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29285938/novel-kunitz-like-peptides-discovered-in-the-zoanthid-palythoa-caribaeorum-through-transcriptome-sequencing
#9
Qiwen Liao, Shengnan Li, Shirley Weng In Siu, Binrui Yang, Chen Huang, Judy Yuet-Wa Chan, Jean-Étienne R L Morlighem, Clarence Tsun Ting Wong, Gandhi Rádis-Baptista, Simon Ming-Yuen Lee
Palythoa caribaeorum (class Anthozoa) is a zoanthid that together jellyfishes, hydra, and sea anemones, which are venomous and predatory, belongs to the Phyllum Cnidaria. The distinguished feature in these marine animals is the cnidocytes in the body tissues, responsible for toxin production and injection that are used majorly for prey capture and defense. With exception for other anthozoans, the toxin cocktails of zoanthids have been scarcely studied and are poorly known. Here, on the basis of the analysis of P...
February 2, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29197232/uptake-tissue-distribution-and-toxicity-of-polystyrene-nanoparticles-in-developing-zebrafish-danio-rerio
#10
Jordan A Pitt, Jordan S Kozal, Nishad Jayasundara, Andrey Massarsky, Rafael Trevisan, Nick Geitner, Mark Wiesner, Edward D Levin, Richard T Di Giulio
Plastic pollution is a critical environmental concern and comprises the majority of anthropogenic debris in the ocean, including macro, micro, and likely nanoscale (less than 100nm in at least one dimension) plastic particles. While the toxicity of macroplastics and microplastics is relatively well studied, the toxicity of nanoplastics is largely uncharacterized. Here, fluorescent polystyrene nanoparticles (PS NPs) were used to investigate the potential toxicity of nanoplastics in developing zebrafish (Danio rerio), as well as to characterize the uptake and distribution of the particles within embryos and larvae...
January 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29162049/rna-seq-and-tn-seq-reveal-fitness-determinants-of-vancomycin-resistant-enterococcus-faecium-during-growth-in-human-serum
#11
Xinglin Zhang, Vincent de Maat, Ana M Guzmán Prieto, Tomasz K Prajsnar, Jumamurat R Bayjanov, Mark de Been, Malbert R C Rogers, Marc J M Bonten, Stéphane Mesnage, Rob J L Willems, Willem van Schaik
BACKGROUND: The Gram-positive bacterium Enterococcus faecium is a commensal of the human gastrointestinal tract and a frequent cause of bloodstream infections in hospitalized patients. The mechanisms by which E. faecium can survive and grow in blood during an infection have not yet been characterized. Here, we identify genes that contribute to growth of E. faecium in human serum through transcriptome profiling (RNA-seq) and a high-throughput transposon mutant library sequencing approach (Tn-seq)...
November 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29145436/scrambled-eggs-proteomic-portraits-and-novel-biomarkers-of-egg-quality-in-zebrafish-danio-rerio
#12
Ozlem Yilmaz, Amélie Patinote, Thao Vi Nguyen, Emmanuelle Com, Regis Lavigne, Charles Pineau, Craig V Sullivan, Julien Bobe
Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry...
2017: PloS One
https://www.readbyqxmd.com/read/29125897/image-analysis-of-neural-stem-cell-division-patterns-in-the-zebrafish-brain
#13
Valerio Lupperger, Felix Buggenthin, Prisca Chapouton, Carsten Marr
Proliferating stem cells in the adult body are the source of constant regeneration. In the brain, neural stem cells (NSCs) divide to maintain the stem cell population and generate neural progenitor cells that eventually replenish mature neurons and glial cells. How much spatial coordination of NSC division and differentiation is present in a functional brain is an open question. To quantify the patterns of stem cell divisions, one has to (i) identify the pool of NSCs that have the ability to divide, (ii) determine NSCs that divide within a given time window, and (iii) analyze the degree of spatial coordination...
November 10, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29035725/alterations-to-juvenile-zebrafish-danio-rerio-swim-performance-after-acute-embryonic-exposure-to-sub-lethal-exposures-of-hydraulic-fracturing-flowback-and-produced-water
#14
Erik J Folkerts, Tamzin A Blewett, Yuhe He, Greg G Goss
Hydraulic fracturing flowback and produced water (FPW) is a wastewater produced during fracturing activities in an operating well which is hyper saline and chemically heterogeneous in nature, containing both anthropogenic and petrogenic chemicals. Determination of FPW associated toxicity to embryonic fish is limited, while investigation into how embryonic exposures may affect later life stages is not yet studied. Zebrafish embryos (24hrs post fertilization) were acutely exposed to 2.5% and 5% FPW fractions for either 24 or 48hrs and returned to freshwater...
December 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29017090/combinatory-effects-of-low-concentrations-of-17%C3%AE-etinylestradiol-and-citalopram-on-non-reproductive-behavior-in-adult-zebrafish-danio-rerio
#15
Tove Porseryd, Martin Kellner, Nasim Reyhanian Caspillo, Kristina Volkova, Lubna Elabbas, Shahid Ullah, Håkan Olsén, Patrik Dinnétz, Inger Porsch Hällström
Sewage effluents contain pharmaceuticals, personal care products and industrial chemicals, exposing aquatic organisms to complex mixtures. The consequences of exposure to combinations of different classes of drugs in fish are largely unknown. In this study, we exposed adult zebrafish (Danio rerio) males and females for two weeks to low, environmentally relevant concentrations of the endocrine disrupting chemical 17α-etinylestradiol (EE2 ) and the selective serotonin re-uptake inhibitor (SSRI) citalopram, alone and in combination, and analyzed behaviors of importance for population fitness, scototaxis (light/dark preference), the novel tank test and shoal cohesion...
December 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28966088/retrotransposons-mimic-germ-plasm-determinants-to-promote-transgenerational-inheritance
#16
Bhavana Tiwari, Paula Kurtz, Amanda E Jones, Annika Wylie, James F Amatruda, Devi Prasad Boggupalli, Graydon B Gonsalvez, John M Abrams
Retrotransposons are a pervasive class of mobile elements present in the genomes of virtually all forms of life [1, 2]. In metazoans, these are preferentially active in the germline, which, in turn, mounts defenses that restrain their activity [3, 4]. Here we report that certain classes of retrotransposons ensure transgenerational inheritance by invading presumptive germ cells before they are formed. Using sensitized Drosophila and zebrafish models, we found that diverse classes of retrotransposons migrate to the germ plasm, a specialized region of the oocyte that prefigures germ cells and specifies the germline of descendants in the fertilized egg...
October 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28919937/gene-meter-accurate-abundance-calculations-of-gene-expression
#17
Alexander E Pozhitkov, Peter A Noble
We previously reported that thousands of transcripts in the mouse and zebrafish significantly increased in abundance in a time series spanning from life to several days after death. Transcript abundances were determined by: calibrating each microarray probe using a dilution series of pooled RNAs, fitting the probe-responses to adsorption models, and back-calculating abundances using the probe signal intensity of a sample and the best fitting model. The accuracy of the abundance measurements was not assessed in our previous study because individual transcript concentrations in the calibration pool were not known...
2017: Communicative & Integrative Biology
https://www.readbyqxmd.com/read/28838034/fate-and-effects-of-silver-nanoparticles-on-early-life-stage-development-of-zebrafish-danio-rerio-in-comparison-to-silver-nitrate
#18
Sébastien Cambier, Marthe Røgeberg, Anastasia Georgantzopoulou, Tommaso Serchi, Camilla Karlsson, Steven Verhaegen, Tore-Geir Iversen, Cédric Guignard, Marcin Kruszewski, Lucien Hoffmann, Jean-Nicolas Audinot, Erik Ropstad, Arno C Gutleb
The use of silver nanomaterials in everyday products, such as cosmetics, textiles, certain types of packaging, etc. is increasing, leading to their release into the environment, including aquatic ecosystems. This last point initiated this investigation on the toxicological effects of Ag nanoparticles (Ag NPs) in the aquatic model organism Danio rerio. For this purpose, zebrafish larvae were exposed to 20nm bare Ag NPs at different concentrations and AgNO3 , used as a positive control for Ag+ ions toxicity, at the beginning of their foraging behaviour to determine adverse effects on fitness parameters...
January 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28814724/pathogenic-bacteria-enhance-dispersal-through-alteration-of-drosophila-social-communication
#19
Ian W Keesey, Sarah Koerte, Mohammed A Khallaf, Tom Retzke, Aurélien Guillou, Ewald Grosse-Wilde, Nicolas Buchon, Markus Knaden, Bill S Hansson
Pathogens and parasites can manipulate their hosts to optimize their own fitness. For instance, bacterial pathogens have been shown to affect their host plants' volatile and non-volatile metabolites, which results in increased attraction of insect vectors to the plant, and, hence, to increased pathogen dispersal. Behavioral manipulation by parasites has also been shown for mice, snails and zebrafish as well as for insects. Here we show that infection by pathogenic bacteria alters the social communication system of Drosophila melanogaster...
August 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28804037/the-longitudinal-effects-of-early-developmental-cadmium-exposure-on-conditioned-place-preference-and-cardiovascular-physiology-in-zebrafish
#20
Marissa Wold, Myranda Beckmann, Shelby Poitra, Ana Espinoza, Robert Longie, Erik Mersereau, Diane C Darland, Tristan Darland
Cadmium (Cd) is a naturally occurring trace metal that is widely considered to be highly toxic to aquatic organisms and a significant health hazard to humans (Amzal et al., 2009; Bernhoft 2013; Burger, 2008; Satarug et al., 2009). The zebrafish (Danio rerio) has been used as a model organism for toxicological studies with Cd (Banni et al., 2011; Blechinger et al., 2007; Chow et al., 2009; Chow et al., 2008; Favorito et al., 2011; Kusch et al., 2007; Matz et al., 2007; Wang and Gallagher, 2013). We asked what the lasting longitudinal effects would be from short early developmental Cd exposure (between 24 and 96h post-fertilization) in a range that larvae might experience living atop typical Cd-containing surface sediments (0, 0...
October 2017: Aquatic Toxicology
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