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maldi mrsa

Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
Daniel D Rhoads, Hannah Wang, James Karichu, Sandra S Richter
The class A mec gene complex also carries the psm-mec gene. The psm-mec gene product can be detected as a peak near 2415m/z using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The correlation of a 2415m/z peak with methicillin resistance (mecA carriage) in consecutive staphylococcal blood culture isolates was evaluated. A 2415±2.00m/z peak was observed in 37% (51/137) of methicillin-resistant Staphylococcus aureus, in none (0/146) of the methicillin-susceptible S...
November 2016: Diagnostic Microbiology and Infectious Disease
D J Biedenbach, J P Iaconis, D F Sahm
OBJECTIVES: Ceftaroline fosamil is indicated for the treatment of community-acquired bacterial pneumonia and ceftriaxone has an indication for lower respiratory tract infections. This study was conducted to compare the relative in vitro activities of these two agents against bacterial species associated with community-associated respiratory tract infections. METHODS: In all, 13 005 isolates of Staphylococcus aureus, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae were collected in 2012-14 from 39 countries in the Asia-Pacific region, Europe, Latin America and Africa-Middle East from respiratory tract specimens...
August 23, 2016: Journal of Antimicrobial Chemotherapy
James A Karlowsky, Douglas J Biedenbach, Samuel K Bouchillon, Meredith Hackel, Joseph P Iaconis, Daniel F Sahm
The objective of this report was to document antimicrobial susceptibility testing surveillance data for ceftaroline and comparative agents from the AWARE (Assessing Worldwide Antimicrobial Resistance Evaluation) global surveillance program for bacterial pathogens causing skin and soft tissue and respiratory tract infections in African and Middle Eastern countries from 2012 through 2014. Pathogen identities were confirmed by MALDI-TOF and antimicrobial susceptibility testing performed by CLSI broth microdilution methodology in a central laboratory...
October 2016: Diagnostic Microbiology and Infectious Disease
Ayman Elbehiry, Musaad Al-Dubaib, Eman Marzouk, Salama Osman, Husam Edrees
: This study was designed to evaluate the ability of MALDI Biotyper (MBT) compared with Vitek(™) 2 compact system for accurate identification of Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS) strains and discriminate methicillin-sensitive S. aureus (MSSA) from methicillin-resistant S. aureus (MRSA). Throughout Al-Qassim region, Saudi Arabia, a total of 198 isolates of S. aureus (132 MSSA and 66 MRSA) and 44 CNS were collected from five dairy farms where the prevalence of staphylococcal mastitis was reported...
July 1, 2016: MicrobiologyOpen
Deborah Steensels, Ariane Deplano, Olivier Denis, Anne Simon, Alexia Verroken
BACKGROUND: The early detection of a methicillin-resistant Staphylococcus aureus (MRSA) outbreak is decisive to control its spread and rapidly initiate adequate infection control measures. Therefore, prompt determination of epidemiologic relatedness of clinical MRSA isolates is essential. Genetic typing methods have a high discriminatory power but their availability remains restricted. In this study, we aimed to challenge matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a typing tool of a nosocomial MRSA outbreak in a neonatal intensive care unit...
June 24, 2016: Acta Clinica Belgica
Y A Prada, F Guzmán, P Rondón, P Escobar, C Ortíz, D A Sierra, R Torres, E Mejía-Ospino
In this work, we performed the rational design of a cationic antimicrobial peptide, GIBIMPY4, using the software DEPRAMPs developed at the GIBIM research group. GIBIMPY4 has a length of 17 amino acids, it is amphipathic, its structure is α-helix and it has a net charge of (+5). Solid-phase peptide synthesis was performed using the Fmoc strategy in acid medium. The primary structure was confirmed by MALDI-TOF mass spectrometry. The antimicrobial activity of the peptide was evaluated by broth microdilution method by measuring optical density in 96-well microplates...
September 2016: Probiotics and Antimicrobial Proteins
Johannes Andries Delport, Ivor Mohorovic, Sandi Burn, John Kenneth McCormick, David Schaus, Robert Lannigan, Michael John
Meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection is responsible for significant morbidity, with mortality rates as high as 60 % if not treated appropriately. We describe a rapid method to detect MRSA in blood cultures using a combined three-hour short-incubation BRUKER matrix-assisted laser desorption/ionization time-of-flight MS BioTyper protocol and a qualitative immunochromatographic assay, the Alere Culture Colony Test PBP2a detection test. We compared this combined method with a molecular method detecting the nuc and mecA genes currently performed in our laboratory...
July 2016: Journal of Medical Microbiology
Michael D Huband, Mariana Castanheira, David J Farrell, Robert K Flamm, Ronald N Jones, Helio S Sader, Rodrigo E Mendes
The in vitro activity of dalbavancin was evaluated against 9303 Staphylococcus aureus and 2670 streptococci, including multidrug-resistant (MDR) isolates, collected from hospitalised patients in Europe and surrounding regions from 2011 to 2013. Dalbavancin recently received approval for the treatment of acute bacterial skin and skin-structure infections by the US Food and Drug Administration (FDA) and the European Medicines Agency. Bacterial identification was confirmed by standard microbiological methods (including MALDI-TOF), and susceptibility testing was performed by reference broth microdilution methods...
June 2016: International Journal of Antimicrobial Agents
Marlène Sauget, Nathalie van der Mee-Marquet, Xavier Bertrand, Didier Hocquet
Within the last decade methicillin-resistant Staphylococcus aureus belonging to CC398 has become a worldwide threat associated with livestock. More recently, methicillin-susceptible S. aureus (MSSA) belonging to CC398 have been increasingly reported as a cause of invasive infections in patients without livestock contact. It appears therefore necessary to implement a convenient tool for the surveillance this emerging pathogen. We evaluated the MALDI-TOF MS as a tool for rapid detection of S. aureus CC398. We used 626 S...
August 2016: Journal of Microbiological Methods
Funmilola Abidemi Ayeni, Tombari Gbarabon, Camilla Andersen, Niels Norskov-Lauritsen
BACKGROUND: Staphylococcus aureus is often responsible for fatal infections and recent upsurge of resistant strains has resulted in therapeutic failure. The identification of this microorganism is a major challenge to medical microbiologists in developing countries. METHODS: One hundred and eighty five isolates which had been previously isolated from the nares of 185 healthy college students' volunteers in Amassoma, Bayelsa State, South Nigeria were identified by MALDI TOF mass spectrometry, and PCR amplification of the spa gene...
December 2015: African Health Sciences
Paula Pescador, María Pilar Romero-Gómez, Rosa Gómez Gil, Jesús Mingorance
No abstract text is available yet for this article.
February 15, 2016: Enfermedades Infecciosas y Microbiología Clínica
Aneta Nowakiewicz, Grażyna Ziółkowska, Przemysław Zięba, Sebastian Gnat, Katarzyna Wojtanowicz-Markiewicz, Aleksandra Trościańczyk
The aim of the study was molecular analysis of coagulase-positive isolates of Staphylococcus bacteria obtained from wild animals and evaluation of their resistance to antimicrobial agents. A total of 76 rectal swabs were taken from wild animals. The species of the Staphylococcus isolates was determined by MALDI TOF MS, susceptibility to antimicrobials was evaluated by phenotypic and molecular methods, epidemiological analysis (ADSRRS-fingerprinting) was also carried out. MRSA isolate was typed by MLST and spa-typing...
February 2016: Comparative Immunology, Microbiology and Infectious Diseases
Cheryl A Mather, Brian J Werth, Shobini Sivagnanam, Dhruba J SenGupta, Susan M Butler-Wu
Vancomycin is the standard of care for the treatment of invasive methicillin-resistantStaphylococcus aureus(MRSA) infections. Infections with vancomycin-nonsusceptible MRSA, including vancomycin-intermediateS. aureus(VISA) and heterogeneous VISA (hVISA), are clinically challenging and are associated with poor patient outcomes. The identification of VISA in the clinical laboratory depends on standard susceptibility testing, which takes at least 24 h to complete after isolate subculture, whereas hVISA is not routinely detected in clinical labs...
April 2016: Journal of Clinical Microbiology
Stephanie S Buehler, Bereneice Madison, Susan R Snyder, James H Derzon, Nancy E Cornish, Michael A Saubolle, Alice S Weissfeld, Melvin P Weinstein, Edward B Liebow, Donna M Wolk
BACKGROUND: Bloodstream infection (BSI) is a major cause of morbidity and mortality throughout the world. Rapid identification of bloodstream pathogens is a laboratory practice that supports strategies for rapid transition to direct targeted therapy by providing for timely and effective patient care. In fact, the more rapidly that appropriate antimicrobials are prescribed, the lower the mortality for patients with sepsis. Rapid identification methods may have multiple positive impacts on patient outcomes, including reductions in mortality, morbidity, hospital lengths of stay, and antibiotic use...
January 2016: Clinical Microbiology Reviews
James A Karlowsky, Douglas J Biedenbach, Samuel K Bouchillon, Joseph P Iaconis, Edina Reiszner, Daniel F Sahm
OBJECTIVES: The objective of this study was to analyse antimicrobial susceptibility testing data generated by the Assessing Worldwide Antimicrobial Resistance Evaluation (AWARE) global surveillance programme for pathogens causing skin and soft tissue infections (SSTIs) in European countries in 2012. METHODS: Confirmation of pathogen identity by MALDI-TOF and antimicrobial susceptibility testing following the CLSI broth microdilution method were performed by a central laboratory...
January 2016: Journal of Antimicrobial Chemotherapy
M Camoez, J M Sierra, M A Dominguez, M Ferrer-Navarro, J Vila, I Roca
Early identification of methicillin-resistant Staphylococcus aureus (MRSA) dominant clones involved in infection and initiation of adequate infection control measures are essential to limit MRSA spread and understand MRSA population dynamics. In this study we evaluated the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) for the automated discrimination of the major MRSA lineages (clonal complexes, CC) identified in our hospital during a 20-year period (1990-2009)...
February 2016: Clinical Microbiology and Infection
Nichole Haag, Kimberly Velk, Tyler McCune, Chun Wu
Methicillin/multiple-resistant Staphylococcus aureus (MRSA) are infectious bacteria that are resistant to common antibiotics. A previous in silico study in our group has identified a hypothetical protein SAV1226 as one of the potential drug targets. In this study, we reported the bioinformatics characterization, as well as cloning, expression, purification and kinetic assays of hypothetical protein SAV1226 from methicillin/vancomycin-resistant Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis revealed a low degree of structural similarity with known proteins...
June 2015: World Academy of Science, Engineering and Technology
Claus Østergaard, Sanne G K Hansen, Jens K Møller
Fast and reliable discrimination of methicillin-resistant Staphylococcus aureus (MRSA) isolates is essential in identifying an outbreak. Molecular typing methods, such as S. aureus protein A (spa) typing, multi locus sequence typing (MLST) and pulse field gel electrophoresis (PFGE) are generally used for this purpose. These methods are all relatively time-consuming and not performed routinely in all laboratories. The aim of this study is to examine whether MALDI-TOF MS can be used as a fast, simple and easily implemented method for first-line discrimination of MRSA isolates...
December 2015: International Journal of Medical Microbiology: IJMM
Tingting Zhang, Jinya Ding, Xiancai Rao, Jingbo Yu, Meiling Chu, Wei Ren, Lu Wang, Wencheng Xue
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with nosocomial infections in many countries. Multilocus sequence typing (MLST) is one of the genetic typing methods used to type MRSA with a high discriminatory power, however, it is labor-intensive, timely, and costly. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) coupled with ClinProTools is a potential tool to discover biomarker peaks and to generate a classification model based on highly sophisticated mathematical algorithms to discriminate clonal lineages...
October 2015: Journal of Microbiological Methods
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