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https://www.readbyqxmd.com/read/29605257/antimicrobial-and-anticancer-potential-of-low-molecular-weight-polypeptides-extracted-and-characterized-from-leaves-of-azadirachta-indica
#1
Mohammed Al Saiqali, Anjana Devi Tangutur, Chandrasekhar Banoth, Bhima Bhukya
Low molecular weight antimicrobial polypeptides were extracted and purified from the young fresh leaves of Azadirachta indica (neem). The total protein extracted was precipitated with 15% TCA-Acetone. The total purified proteins yielded from the two extraction methods were 122.33 ± 2.21 and 115.09 ± 1.88 mg/g of the total fresh weight. The SDS-PAGE analysis identified the presence of eight low molecular weight polypeptide bands. The antimicrobial activity of the resolved bands was detected by Polyacrylamide gel-Agar overlay diffusion assay (PAG-ADA)...
March 29, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29534106/a-new-scheme-for-strain-typing-of-methicillin-resistant-staphylococcus-aureus-on-the-basis-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-using-machine-learning-approach
#2
Hsin-Yao Wang, Tzong-Yi Lee, Yi-Ju Tseng, Tsui-Ping Liu, Kai-Yao Huang, Yung-Ta Chang, Chun-Hsien Chen, Jang-Jih Lu
Methicillin-resistant Staphylococcus aureus (MRSA), one of the most important clinical pathogens, conducts an increasing number of morbidity and mortality in the world. Rapid and accurate strain typing of bacteria would facilitate epidemiological investigation and infection control in near real time. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and cost-effective tool for presumptive strain typing. To develop robust method for strain typing based on MALDI-TOF spectrum, machine learning (ML) is a promising algorithm for the construction of predictive model...
2018: PloS One
https://www.readbyqxmd.com/read/29524153/small-mammals-as-sentinels-of-antimicrobial-resistant-staphylococci
#3
Vladimír Kmeť, Anna Čuvalová, Michal Stanko
A total of 39 coagulase-negative staphylococci and seven Staphylococcus aureus strains were isolated from small mammal feces, i.e., the striped field mouse (Apodemus agrarius) and the yellow-necked mouse (A. flavicollis) in two sampling areas, deciduous forest and karst plains. MALDI-TOF analysis revealed five species of coagulase-negative staphylococci: S. sciuri, S. hominis, S. warneri, S. haemolyticus, and S. xylosus. All strains were susceptible to tetracycline, linezolid, vancomycin, and teicoplanin. Three MRSA strains with the mecA gene were detected...
March 9, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29522576/rapid-and-easy-detection-of-low-level-resistance-to-vancomycin-in-methicillin-resistant-staphylococcus-aureus-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#4
Kota Asakura, Takuya Azechi, Hiroshi Sasano, Hidehito Matsui, Hideaki Hanaki, Motoyasu Miyazaki, Tohru Takata, Miwa Sekine, Tomoiku Takaku, Tomonori Ochiai, Norio Komatsu, Keigo Shibayama, Yuki Katayama, Koji Yahara
Vancomycin-intermediately resistant Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) are associated with treatment failure. hVISA contains only a subpopulation of cells with increased minimal inhibitory concentrations, and its detection is problematic because it is classified as vancomycin-susceptible by standard susceptibility testing and the gold-standard method for its detection is impractical in clinical microbiology laboratories. Recently, a research group developed a machine-learning classifier to distinguish VISA and hVISA from vancomycin-susceptible S...
2018: PloS One
https://www.readbyqxmd.com/read/29180272/combination-of-maldi-tof-ms-and-pbp2-latex-agglutination-assay-for-rapid-mrsa-detection
#5
Marianna Ábrók, Andrea Lázár, Mária Szécsényi, Judit Deák, Edit Urbán
Sample preparation was optimized for MALDI-TOF MS directly from the selective enrichment broth to detect Staphylococcus aureus. A combination of MALDI-TOF MS and the PBP2' latex agglutination assay was applied for MRSA screening and evaluated on 255 clinical samples. MRSA colonisation can be reported already 18-24h after sample collection.
January 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29080625/diversity-of-livestock-associated-methicillin-resistant-staphylococcus-aureus
#6
Henok A Tegegne, Ivana Koláčková, Renata Karpíšková
OBJECTIVE: To evaluate the diversity and molecular characteristics of livestock associated methicillin-resistant Staphylococcus aureus in livestock animals, food of animal origin and the environment in the Czech Republic. METHODS: After having been primarily enriched in buffered peptone water, the samples were cultured on Baird-Parker agar. Presumptive colonies were sub-cultured to blood agar and assessed morphologically. Furthermore, presumptive Staphylococcus aureus colonies were confirmed by MALDI-TOF...
September 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28954959/rapid-discrimination-between-methicillin-sensitive-and-methicillin-resistant-staphylococcus-aureus-using-maldi-tof-mass-spectrometry
#7
Kazuyuki Sogawa, Masaharu Watanabe, Takayuki Ishige, Syunsuke Segawa, Akiko Miyabe, Syota Murata, Tomoko Saito, Akihiro Sanda, Katsunori Furuhata, Fumio Nomura
 Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major pathogens responsible for nosocomial infections. The presence of MRSA in a hospital is detrimental to patients and to hospital management. Thus, rapid identification of MRSA is needed. Here, we report on a prospective method to rapidly discriminate of MSSA from MRSA using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and support vector machine (SVM) analysis in 160 clinical isolates of S. aureus...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28924947/development-of-a-rapid-maldi-tof-ms-based-epidemiological-screening-method-using-mrsa-as-a-model-organism
#8
Åsa Lindgren, Nahid Karami, Roger Karlsson, Christina Åhrén, Martin Welker, Edward R B Moore, Liselott Svensson Stadler
In this study we present a method using whole cell MALDI-TOF MS and VITEK MS RUO/SARAMIS as a rapid epidemiological screening tool. MRSA was used as a model organism for setting up the screening strategy. A collection of well-characterised MRSA strains representing the 19 most common Pulsed-Field Gel Electrophoresis (PFGE)-types in the region of South-West Sweden for the past 20 years was analysed with MALDI-TOF MS. A total of 111 MRSA strains were used for creating 19 PFGE-specific Superspectra using VITEK MS RUO/SARAMIS...
January 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28900246/maldi-tof-ms-based-identification-and-molecular-characterization-of-food-associated-methicillin-resistant-staphylococcus-aureus
#9
H M Manukumar, S Umesha
Food-borne methicillin resistant Staphylococcus aureus (MRSA) is involved in two-fold higher mortality rate compared to methicillin susceptible S. aureus (MSSA). Eventhough Mysuru recognized as cleanest city in the world, prevalence of food contamination is not detailed. The aim is to screen food samples from Mysuru area and to characterize MRSA strain, employing MALDI-Biotyper, multiplex PCR to distinguish between MRSA and MSSA by PCR-coupled single strand conformation polymorphism (PCR-SSCP). Of all the food-borne pathogens, S...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28887200/purification-and-characterisation-of-a-novel-antistaphylococcal-peptide-asp-1-from-bacillus-sp-urid-12-1
#10
Ajay Ghosh Chalasani, Utpal Roy, Sushma Nema
A strong antistaphylococcal peptide (ASP-1) from Bacillus subtilis URID 12.1 strain that is active against cefoxitin- and methicillin-resistant Staphylococcus aureus clinical isolates was purified to homogeneity by solvent extraction, silica gel-based adsorption chromatography and reversed-phase high-performance liquid chromatography. The peptide sequence of ASP-1 as determined by MALDI-TOF/MS and ESI-FTICR-MS was acetylated Phe-Thr-Ala-Val-Dhb-Phe-Ile/Leu. The peptide was further analysed by alkaline hydrolysis, ESI-Q-TOF-MS and an ion mobility assay, which detected the presence of a lactone ring in the intact peptide and a cyclic nature, subsequently revealing the linearised peptide sequence as acPhe-Leu-Phe-Thr-Val-Ala-Dhb...
January 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28831593/impact-of-rapid-diagnosis-of-staphylococcus-aureus-bacteremia-from-positive-blood-cultures-on-patient-management
#11
M P Romero-Gómez, E Cendejas-Bueno, J García Rodriguez, J Mingorance
We have performed a retrospective, before-after comparison of turnaround time and therapy adjustment parameters before and after the introduction of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) plus mecA polymerase chain reaction (PCR) for the identification of methicillin-resistant Staphylococcus aureus (MRSA) in positive blood cultures. There were 227 episodes of S. aureus bacteremia during the study periods. The pre-MALDI-TOF and post-MALDI-TOF groups included 133 and 94 patients, respectively...
December 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28430811/antimicrobial-resistance-in-coagulase-positive-staphylococci-isolated-from-companion-animals-in-australia-a-one-year-study
#12
Sugiyono Saputra, David Jordan, Kate A Worthing, Jacqueline M Norris, Hui S Wong, Rebecca Abraham, Darren J Trott, Sam Abraham
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#13
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28316631/potential-impact-of-rapid-blood-culture-testing-for-gram-positive-bacteremia-in-japan-with-the-verigene-gram-positive-blood-culture-test
#14
Ken Kikuchi, Mari Matsuda, Shigekazu Iguchi, Tomonori Mizutani, Keiichi Hiramatsu, Michiru Tega-Ishii, Kaori Sansaka, Kenta Negishi, Kimie Shimada, Jun Umemura, Shigeyuki Notake, Hideji Yanagisawa, Hiroshi Takahashi, Reiko Yabusaki, Hideki Araoka, Akiko Yoneyama
Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28091797/mass-spectrometric-identification-of-phenol-soluble-modulins-in-the-atcc%C3%A2-43300-standard-strain-of-methicillin-resistant-staphylococcus-aureus-harboring-two-distinct-phenotypes
#15
K S Jang, M Park, J Y Lee, J S Kim
Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300™) is a representative methicillin-resistant S. aureus (MRSA) strain that is used as a quality control strain for testing the methicillin susceptibility of clinical isolates. It has been consistently observed that colonies with two different colors (golden yellow and white) grow from the ATCC stock on blood agar plates. In this study, staphylococcal peptide and protein profiling was performed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry...
July 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27896481/high-perineal-and-overall-frequency-of-staphylococcus-aureus-in-people-who-inject-drugs-compared-to-non-injectors
#16
Disa Dahlman, Farnaz Jalalvand, Marianne Alanko Blomé, Anders Håkansson, Håkan Janson, Susanne Quick, Anna C Nilsson
To investigate the prevalence, distribution, and colonization burden of Staphylococcus aureus (S. aureus) and MRSA in different body sites among people who inject drugs (PWID) and compare it to a control group consisting of non-injectors. In this cross-sectional survey, 49 active PWID from the needle exchange program (NEP) in Malmö, Sweden, and 60 non-injecting controls from an emergency psychiatric inpatient ward at Malmö Addiction Centre were tested for S. aureus (including MRSA) by culture, PCR, and MALDI-TOF...
February 2017: Current Microbiology
https://www.readbyqxmd.com/read/27866257/detection-of-methicillin-resistant-staphylococcus-aureus-using-phage-amplification-combined-with-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#17
Jon C Rees, John R Barr
Antibiotic resistance continues to contribute significantly to morbidity and mortality across the world. Developing new tests for antibiotic-resistant bacteria is a core action to combat resistant infections. We describe a method that uses phage amplification detection (PAD) combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to rapidly identify Staphylococcus aureus and determine phenotypic susceptibility to cefoxitin. Samples tested for S. aureus are incubated together with bacteriophage in the presence and absence of cefoxitin and subjected to rapid trypsin digestion followed by MALDI-MS analysis...
February 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27598775/low-enteric-colonization-with-multidrug-resistant-pathogens-in-soldiers-returning-from-deployments-experience-from-the-years-2007-2015
#18
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
https://www.readbyqxmd.com/read/27568365/the-presence-of-a-single-maldi-tof-mass-spectral-peak-predicts-methicillin-resistance-in-staphylococci
#19
Daniel D Rhoads, Hannah Wang, James Karichu, Sandra S Richter
The class A mec gene complex also carries the psm-mec gene. The psm-mec gene product can be detected as a peak near 2415m/z using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The correlation of a 2415m/z peak with methicillin resistance (mecA carriage) in consecutive staphylococcal blood culture isolates was evaluated. A 2415±2.00m/z peak was observed in 37% (51/137) of methicillin-resistant Staphylococcus aureus, in none (0/146) of the methicillin-susceptible S...
November 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27559120/comparative-in-vitro-activities-of-ceftaroline-and-ceftriaxone-against-bacterial-pathogens-associated-with-respiratory-tract-infections-results-from-the-aware-surveillance-study
#20
D J Biedenbach, J P Iaconis, D F Sahm
OBJECTIVES: Ceftaroline fosamil is indicated for the treatment of community-acquired bacterial pneumonia and ceftriaxone has an indication for lower respiratory tract infections. This study was conducted to compare the relative in vitro activities of these two agents against bacterial species associated with community-associated respiratory tract infections. METHODS: In all, 13 005 isolates of Staphylococcus aureus, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae were collected in 2012-14 from 39 countries in the Asia-Pacific region, Europe, Latin America and Africa-Middle East from respiratory tract specimens...
December 2016: Journal of Antimicrobial Chemotherapy
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