keyword
https://read.qxmd.com/read/37546336/antimicrobial-resistance-and-its-relationship-with-antimicrobial-use-on-austrian-dairy-farms
#21
JOURNAL ARTICLE
Thomas Werner, Annemarie Käsbohrer, Barbara Wasner, Sandra Köberl-Jelovcan, Sebastian G Vetter, Christa Egger-Danner, Klemens Fuchs, Walter Obritzhauser, Clair L Firth
The aim of this study was to investigate the prevalence of ESBL/AmpC-producing E. coli and the resistance pattern of commensal E. coli , as well as the link between the use of antibiotics (AMU) and the occurrence of resistance in E. coli on Austrian dairy farms. AMU data from 51 farms were collected over a one-year period in 2020. Fecal samples were collected from cows, pre-weaned and weaned calves in 2020 and 2022. Samples were then analyzed using non-selective and selective agar plates, E. coli isolates were confirmed by MALDI-TOF analysis...
2023: Frontiers in Veterinary Science
https://read.qxmd.com/read/37526891/prevalence-of-ampc-esbl-and-colistin-resistance-genes-in-enterobacterales-isolated-from-ready-to-eat-food-in-algeria
#22
JOURNAL ARTICLE
Nawel Zaatout, Ahmad I Al-Mustapha, Amira Bouaziz, Rima Ouchene, Annamari Heikinheimo
Antimicrobial resistance among bacteria present in ready-to-eat foods is an emerging concern. Hence, this study investigated the presence of extended-spectrum and AmpC β-lactamases (ESBL/AmpC)-producing Enterobacterales (ESBL-E) and the dissemination of mcr-1 in ESBL-E from ready-to-eat food samples (RTE) in Algeria. RTE food samples (n = 204) were aseptically collected and selectively cultured using MacConkey agar. The isolates were screened for ESBL production using the DDST test, confirmed ESBL-E isolates were identified using different conventional methods and MALDI-TOF MS, antibiotic susceptibility was determined using the disc diffusion and broth microdilution assay, ESBL-E isolates were analyzed for colistin and ESBL/AmpC encoding genes by PCR, and food samples were analyzed by univariate and multiple logistic regression...
August 1, 2023: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/37481113/genomic-characterisation-of-multi-drug-resistant-escherichia-coli-and-klebsiella-pneumoniae-co-harbouring-mcr-1-and-mcr-3-genes-on-a-single-plasmid-from-paediatric-clinical-cases
#23
JOURNAL ARTICLE
Sandip Patil, Liu Pai, Xiowen Chen, Ngiambudulu M Francisco, Hongyu Chen, Yunsheng Chen, Shaowei Dong, Sixi Liu, Feiqiu Wen
BACKGROUND: Emergence of plasmid-born mobile colistin resistance (mcr) gene, a growing concern in healthcare. Therefore, this study aims to genomically characterize multidrug-resistant Escherichia coli and Klebsiella pneumoniae co-harboring the mcr-1 and mcr-3 genes in young children. METHODS: E. coli (n=3) and K. pneumoniae (n=2) were collected from abdominal secretion and blood, respectively. The isolates were screened using tryptone soya broth (TSB) with 4µl/ml polymyxin-B...
July 20, 2023: Journal of Global Antimicrobial Resistance
https://read.qxmd.com/read/37405539/multi-instrumental-analysis-toward-exploring-the-diabetic-foot-infection-microbiota
#24
JOURNAL ARTICLE
Michał Złoch, Ewelina Maślak, Wojciech Kupczyk, Paweł Pomastowski
The polymicrobial nature of diabetic foot infection (DFI) makes accurate identification of the DFI microbiota, including rapid detection of drug resistance, challenging. Therefore, the main objective of this study was to apply matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technique accompanied by multiply culture conditions to determine the microbial patterns of DFIs, as well as to assess the occurrence of drug resistance among Gram-negative bacterial isolates considered a significant cause of the multidrug resistance spread...
July 5, 2023: Current Microbiology
https://read.qxmd.com/read/37403520/low-prevalence-of-antimicrobial-resistant-organisms-methicillin-resistant-staphylococcus-aureus-extended-beta-lactamase-producing-enterobacteriaceae-and-vancomycin-resistant-enterococci-in-bulk-tank-milk-in-new-south-wales-australia
#25
JOURNAL ARTICLE
S Rowe, C Cunningham, L Ingenhoff, J M Norris, R N Zadoks
OBJECTIVE: Estimate the presence of methicillin resistant Staphylococcus aureus (MRSA), extended beta-lactamase (ESBL) producing Enterobacteriaceae, and vancomycin resistant enterococci (VRE) in bulk tank milk in dairy herds in New South Wales (NSW), Australia. METHODS: Bulk tank milk samples (n = 80) were collected from dairy farms (n = 40, i.e. 2 per farm) in NSW during 2021. Bacteria were cultured using selective chromogenic indicator media with isolate identity confirmed using biochemical testing, Gram stain, and MALDI-TOF mass spectroscopy...
July 5, 2023: Australian Veterinary Journal
https://read.qxmd.com/read/37370382/evaluation-of-aztreonam-and-ceftazidime-avibactam-synergism-against-klebsiella-pneumoniae-by-maldi-tof-ms
#26
JOURNAL ARTICLE
Camila Mörschbächer Wilhelm, Everton Inamine, Andreza Francisco Martins, Afonso Luís Barth
INTRODUCTION: Resistance to carbapenems due to the co-production of NDM and ESBL or NDM and KPC is increasing. Therefore, combined therapy with aztreonam (ATM) plus ceftazidime/avibactam (CZA) has been recommended. Then, it is necessary to develop and evaluate fast and simple methods to determine synergism in vitro in microbiology laboratories. OBJECTIVE: To develop a method to determine the synergism of ATM and CZA by MALDI-TOF MS (SynMALDI). METHOD: Klebsiella pneumoniae (n = 22) isolates with bla NDM and/or bla KPC genes were tested...
June 16, 2023: Antibiotics
https://read.qxmd.com/read/37356524/multidrug-resistant-enterobacter-spp-in-wastewater-and-surface-water-molecular-characterization-of-%C3%AE-lactam-resistance-and-metal-tolerance-genes
#27
JOURNAL ARTICLE
Guilherme Sgobbi Zagui, Natália Columbaro Moreira, Danilo Vitorino Santos, Cristina Filomena Pereira Rosa Paschoalato, Jordi Sierra, Martí Nadal, José L Domingo, Ana Lúcia Costa Darini, Leonardo Neves Andrade, Susana Inés Segura-Muñoz
Among the ESKAPE group pathogens, Enterobacter spp. is an opportunistic Gram-negative bacillus, widely dispersed in the environment, that causes infections. In the present study, samples of hospital wastewater, raw and treated urban wastewater, as well as surface receiving water, were collected to assess the occurrence of multidrug-resistant (MDR) Enterobacter spp. A molecular characterization of β-lactam antibiotic resistance and metal tolerance genes was performed. According to identification by MALDI-TOF MS, 14 isolates were obtained: 7 E...
June 23, 2023: Environmental Research
https://read.qxmd.com/read/37336594/characterization-of-antibiotic-resistance-genes-and-mobile-elements-in-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-strains-isolated-from-hospitalized-patients-in-guangdong-china
#28
JOURNAL ARTICLE
Muhammad Shafiq, Hazrat Bilal, Budi Permana, Danhong Xu, Gengzhong Cai, Xin Li, Mi Zeng, Yumeng Yuan, Xiaoyang Jiao, Fen Yao
AIM: This study aimed to investigate the high-resolution phenotypic and genotypic characterization of extended-spectrum β-lactamase (ESBL)-producing E. coli strains isolated from hospitalized patients to explore the resistance genes and mobile genetic elements (MGEs) involved in horizontal dissemination. METHODS: Between May and September 2021, a total of 216 ESBL-producing E. coli isolates were recovered from multiple departments. The identification of strains was performed using MALDI-TOF mass spectrometry and PCR, while antibiotic susceptibility testing was carried out using the Vitek 2 COMPACT system to determine resistance patterns, while PCR was used to detect different resistance genes and MGEs...
June 19, 2023: Journal of Applied Microbiology
https://read.qxmd.com/read/37318364/escherichia-ruysiae-may-serve-as-a-reservoir-of-antibiotic-resistance-genes-across-multiple-settings-and-regions
#29
JOURNAL ARTICLE
Edgar I Campos-Madueno, Claudia Aldeia, Parham Sendi, Andrea Endimiani
Gut colonization with multidrug-resistant Enterobacterale s (MDR- Ent ) has reached worrisome levels worldwide. In this context, Escherichia ruysiae is a recently described species mostly found in animals. However, its spread and impact on humans is poorly understood. A stool sample from a healthy individual living in India was screened for the presence of MDR- Ent using culture-based methods. Colonies were routinely identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and phenotypically characterized by broth microdilution...
June 15, 2023: Microbiology Spectrum
https://read.qxmd.com/read/37317224/maldi-tof-ms-approaches-for-the-identification-of-the-susceptibility-of-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli
#30
JOURNAL ARTICLE
Yuriko Matsumura, Kazuko Ikegaya
The increase in multidrug-resistant microorganisms that produce extended-spectrum β-lactamases (ESBLs) and carbapenemases is a serious problem worldwide. Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used for the rapid detection of antibiotic-resistant bacteria. The objective of this study was to establish a method to detect ESBL-producing Escherichia coli by monitoring the hydrolyzation of cefotaxime (CTX) using MALDI-TOF MS. According to the ratio of the peak intensity of CTX and hydrolyzed-CTX-related compounds, the ESBL-producing strains could be clearly distinguished after 15 min of incubation...
May 9, 2023: Microorganisms
https://read.qxmd.com/read/37280775/occurrence-of-extended-spectrum-%C3%AE-lactamases-producing-escherichia-coli-isolates-over-gradient-pollution-in-an-urban-tropical-estuary
#31
JOURNAL ARTICLE
Wellington Felipe Costa, Rodolfo Paranhos, Marianne Pataro Mello, Renata Cristina Picão, Marinella Silva Laport
Bacterial resistance to antimicrobials is a global public health problem that surpasses the human context and can be increased by pollution. However, the lack of systematic monitoring of resistance in some aquatic matrices, such as tropical estuaries, makes it unknown whether its occurrence is associated with anthropogenic pollution in these environments. Therefore, we investigated the occurrence of extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli as a resistance indicator for 12 consecutive months at three representative points of a pollution gradient in Guanabara Bay (GB), Brazil...
June 6, 2023: Environmental Microbiology
https://read.qxmd.com/read/37268680/extended-spectrum-beta-lactamases-found-in-escherichia-coli-isolates-obtained-from-blood-cultures-and-corresponding-stool-specimen
#32
JOURNAL ARTICLE
Nina Doerr, Nadine Dietze, Norman Lippmann, Arne C Rodloff
With extended-spectrum β-lactamases (ESBLs) and CTX-M enzymes being on the rise, antimicrobial treatment of enterobacterial infections is becoming more and more challenging. Our study aimed at a molecular characterization of phenotypically ESBL-positive E. coli strains obtained from blood cultures of patients of the University Hospital of Leipzig (UKL), Germany. The presence of CMY-2, CTX-M-14 and CTX-M-15 was investigated using Streck ARM-D Kit (Streck, USA). Real-time amplifications were performed by QIAGEN Rotor-Gene Q MDx Thermocycler (QIAGEN, Thermo Fisher Scientific, USA)...
June 2, 2023: Scientific Reports
https://read.qxmd.com/read/37169125/phenotypic-and-genotypic-characteristics-of-beta-lactamase-dominant-with-carba-ampc-and-esbl-producing-bacteria-in-municipal-wastewater-influent-in-helsinki-finland
#33
JOURNAL ARTICLE
Ananda Tiwari, Jaana Paakkanen, Ahmad I Al-Mustapha, Juha Kirveskari, Rene S Hendriksen, Annamari Heikinheimo
OBJECTIVE: Analyzing samples of municipal wastewater influent (before treatment) can help to map the status of antibiotic-resistant bacteria (ARB) at the population level in sewershed communities and may also help in predicting the public health risks of ARB in surface water due to the outfall of wastewater. In this study, we investigated the bacterial isolates carrying beta-lactamase (BL) genes in wastewater and compared their genotypic and phenotypic characteristics. METHODS: A total of 399 bacterial isolates grown on CHROMagarESBL (n = 207) and CHROMagarKPC (n = 192) from composite wastewater influent samples (n = 7) from the Viikinmäki wastewater treatment plant (Helsinki) were sub-cultured, nucleic acid was extracted, and the prevalence of different BL genes was screened with multiplex polymerase chain reaction (PCR)...
May 9, 2023: Journal of Global Antimicrobial Resistance
https://read.qxmd.com/read/37072773/genome-profiling-of-uropathogenic-e-coli-from-strictly-defined-community-acquired-uti-in-paediatric-patients-a-multicentric-study
#34
JOURNAL ARTICLE
Sarita Mohapatra, Dipannita Ghosh, Perumal Vivekanandan, Sneha Chunchanur, Shwetha Venugopal, Vibhor Tak, Rajashree Panigrahy, Susmita Chaudhuri, Swati Pundir, Tanya Sharma, Deepak Kocher, Harpreet Singh, Hitender Gautam, Seema Sood, Bimal Kumar Das, Arti Kapil, Arvind Kumar, Rajesh Kumari, Mani Kalaivani, Ambica Rangaiah, Harshal Ramesh Salve, Sumit Malhotra, Shashi Kant, Pankaj Hari
BACKGROUND: Urinary tract infection (UTI) in children is a common bacterial infection. The emergence of extended-spectrum beta-lactamases (ESBLs) poses a major challenge against the treatment of uropathogens. We aimed to characterize the E. coli isolates recovered from children with UTI for their resistance profile and circulating sequence types (ST). METHODS: Children (> 1.5-18 years of age) from different community health centres of India with symptoms of UTI were enrolled...
April 18, 2023: Antimicrobial Resistance and Infection Control
https://read.qxmd.com/read/37036600/rapid-identification-of-canine-uropathogens-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-the-clinical-factors-that-correlated-bacterial-species-and-antimicrobial-resistance
#35
JOURNAL ARTICLE
Artitaya Pinthanon, Chayanan Nithitarnwat, Chadaporn Pintapin, Chonradee Siripanee, Jitrapa Yindee, Nutthee Am-In, Sawang Kesdangsakonwut, Sirilak Surachetpong, Nuvee Prapasarakul
Using the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) method for bacterial diagnosis, rapid urine sample preparation can reduce time relapsing of diagnosis and improve discriminatory power in coinfection cases. We aimed to evaluate rapid urine preparation procedures before MALDI-TOF MS application using dog clinical urine samples in comparison with standard microbiological diagnostic methods by agreement analysis. We determined the frequency and distribution of bacteria and bacterial resistance and their correlations to clinical history...
April 10, 2023: Veterinary Research Communications
https://read.qxmd.com/read/37030061/microbiological-quality-and-antimicrobial-resistance-of-bacteria-species-recovered-from-ready-to-eat-food-water-samples-and-palm-swabs-of-food-vendors-in-accra-ghana
#36
JOURNAL ARTICLE
Helena Dela, Beverly Egyir, Eric Behene, Hamdiya Sulemana, Rodalyn Tagoe, Ronald Bentil, Richard N A Bongo, Bassirou Bonfoh, Jakob Zinsstag, Langbong Bimi, Kennedy Kwasi Addo
This study sought to investigate microbial quality and antimicrobial resistance of bacteria species from Ready-to-Eat (RTE) food, water, and vendor palm swab samples. Between 2019 and 2020, RTE food, water and vendor palm swab samples were collected from food vending sites in Accra, Ghana. Samples were cultured and confirmed using the Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). Antimicrobial susceptibility testing (AST) was conducted using disk diffusion method...
April 4, 2023: International Journal of Food Microbiology
https://read.qxmd.com/read/37004897/biofilm-formation-and-antibiotic-resistance-profiles-of-water-borne-pathogens
#37
JOURNAL ARTICLE
Kursat Koskeroglu, Mukaddes Barel, Harun Hizlisoy, Yeliz Yildirim
Water sources (surface water, drinking water, rivers, and ponds) are significant reservoirs for transmitting antibiotic-resistant bacteria. In addition, these waters are an important public health problem because they are suitable environments for transferring antibiotic resistance genes between bacterial species. Our study aimed to assess the prevalence of Extended-spectrum beta-lactamase (ESBL) producing isolates in water samples, the susceptibility of the isolates to the specified antibiotics, the determination of biofilm ability, antibiotic resistance genes, and the molecular typing of the isolates...
June 2023: Research in Microbiology
https://read.qxmd.com/read/36986292/bacterial-co-infections-and-antimicrobial-resistance-in-patients-hospitalized-with-suspected-or-confirmed-covid-19-pneumonia-in-kazakhstan
#38
JOURNAL ARTICLE
Alyona Lavrinenko, Svetlana Kolesnichenko, Irina Kadyrova, Anar Turmukhambetova, Lyudmila Akhmaltdinova, Dmitriy Klyuyev
Our study was carried out to characterize respiratory tract microbiota in patients with "COVID-like pneumonia" in Kazakhstan and analyze differences between COVID-19 positive and negative groups. Sputum samples were collected from hospitalized patients, ≥18 years old, in the three cities in Kazakhstan with the highest COVID-19 burden in July 2020. Isolates were identified by MALDI-TOF MS. Susceptibility testing was performed by disk diffusion. We used SPSS 26 and MedCalc 19 for statistical analysis. Among 209 patients with pneumonia, the median age was 62 years and 55% were male...
February 23, 2023: Pathogens
https://read.qxmd.com/read/36906027/first-reported-detection-of-the-mobile-colistin-resistance-genes-mcr-8-and-mcr-9-in-the-irish-environment
#39
JOURNAL ARTICLE
Niamh Cahill, Brigid Hooban, Kelly Fitzhenry, Aoife Joyce, Louise O'Connor, Georgios Miliotis, Francesca McDonagh, Liam Burke, Alexandra Chueiri, Maeve Louise Farrell, James E Bray, Niall Delappe, Wendy Brennan, Deirdre Prendergast, Montserrat Gutierrez, Catherine Burgess, Martin Cormican, Dearbháile Morris
The emergence and dissemination of mobile colistin resistance (mcr) genes across the globe poses a significant threat to public health, as colistin remains one of the last line treatment options for multi-drug resistant infections. Environmental samples (157 water and 157 wastewater) were collected in Ireland between 2018 and 2020. Samples collected were assessed for the presence of antimicrobial resistant bacteria using Brilliance ESBL, Brilliance CRE, mSuperCARBA and McConkey agar containing a ciprofloxacin disc...
March 9, 2023: Science of the Total Environment
https://read.qxmd.com/read/36830166/multicenter-surveillance-of-antimicrobial-resistance-among-gram-negative-bacteria-isolated-from-bloodstream-infections-in-ghana
#40
JOURNAL ARTICLE
Eric S Donkor, Khitam Muhsen, Sherry A M Johnson, Fleischer C N Kotey, Nicholas T K D Dayie, Patience B Tetteh-Quarcoo, Edem M A Tette, Mary-Magdalene Osei, Beverly Egyir, Nicholas I Nii-Trebi, Godfred Owusu-Okyere, Alex Owusu-Ofori, Yonatan Amir, Saritte Perlman, Perdita Hilary Lopes, Adjo Mfodwo, Nicola C Gordon, Louise Gresham, Mark Smolinski, Dani Cohen
BACKGROUND: Antimicrobial resistance (AMR) in Gram-negative bacteria-causing bloodstream infections (BSIs), such as Klebsiella pneumoniae and non-typhoidal Salmonella (NTS), is a major public health concern. Nonetheless, AMR surveillance remains scarce in sub-Saharan Africa, where BSI treatment is largely empirical. The aim of the study was to determine the distribution and AMR patterns of BSI-causing NTS, K. pneumoniae , and other Gram-negative bacteria in Ghana. METHODS: A cross-sectional study was conducted between April and December 2021 at eleven sentinel health facilities across Ghana as part of a pilot study on the feasibility and implementation of the human sector AMR surveillance harmonized protocol in sub-Saharan Africa...
January 27, 2023: Antibiotics
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