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maldi esbl

Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
Mohamed Belmahdi, Sofiane Bakour, Charbel Al Bayssari, Abdelaziz Touati, Jean-Marc Rolain
This study aimed to characterise the molecular support of antibiotic resistance in expanded-spectrum cephalosporin (ESC)-resistant Escherichia coli isolates recovered from healthy broilers in Béjaïa, northeast Algeria. A total of 61 intestinal swabs from slaughtered broilers from four regions in Béjaïa locality, Algeria, were collected between February and April 2014, from which 20 ESC-resistant E. coli strains were isolated. Escherichia coli isolates were identified by classical biochemical and MALDI-TOF methods...
September 2016: Journal of Global Antimicrobial Resistance
James A Karlowsky, Douglas J Biedenbach, Samuel K Bouchillon, Meredith Hackel, Joseph P Iaconis, Daniel F Sahm
The objective of this report was to document antimicrobial susceptibility testing surveillance data for ceftaroline and comparative agents from the AWARE (Assessing Worldwide Antimicrobial Resistance Evaluation) global surveillance program for bacterial pathogens causing skin and soft tissue and respiratory tract infections in African and Middle Eastern countries from 2012 through 2014. Pathogen identities were confirmed by MALDI-TOF and antimicrobial susceptibility testing performed by CLSI broth microdilution methodology in a central laboratory...
October 2016: Diagnostic Microbiology and Infectious Disease
Abdoul-Salam Ouedraogo, Mahamadou Sanou, Aimée Kissou, Soufiane Sanou, Hermann Solaré, Firmin Kaboré, Armel Poda, Salim Aberkane, Nicolas Bouzinbi, Idrissa Sano, Boubacar Nacro, Lassana Sangaré, Christian Carrière, Dominique Decré, Rasmata Ouégraogo, Hélène Jean-Pierre, Sylvain Godreuil
BACKGROUND: Nothing is known about the epidemiology and resistance mechanisms of extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-PE) in Burkina Faso. The objective of this study was to determine ESBL-PE prevalence and to characterize ESBL genes in Burkina Faso. METHODS: During 2 months (June-July 2014), 1602 clinical samples were sent for bacteriologic investigations to the microbiology laboratories of the tree main hospitals of Burkina Faso. Isolates were identified by mass spectrometry using a matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) BioTyper...
2016: BMC Infectious Diseases
Christoph Lübbert, Karolin Wendt, Jürgen Feisthammel, Annette Moter, Norman Lippmann, Thilo Busch, Joachim Mössner, Albrecht Hoffmeister, Arne C Rodloff
BACKGROUND: Plastic stents used for the treatment of biliary obstruction will become occluded over time due to microbial colonization and formation of biofilms. Treatment of stent-associated cholangitis is often not effective because of inappropriate use of antimicrobial agents or antimicrobial resistance. We aimed to assess the current bacterial and fungal etiology of stent-associated biofilms, with particular emphasis on antimicrobial resistance. METHODS: Patients with biliary strictures requiring endoscopic stent placement were prospectively enrolled...
2016: PloS One
Tayfur Demiray, Mehmet Koroglu, Ahmet Ozbek, Mustafa Altindis
BACKGROUND: Severe cases of infections caused by Raoultella planticola are constantly being reported from all over the world with the increase in drug-resistance patterns. In this study, we retrospectively evaluated the clinical characteristics of R. planticola infections with patients' demographics and antimicrobial susceptibilities of the R. planticola isolates. METHODS: R. planticola isolates were retrospectively evaluated. VITEK 2(®) automated system was used for identification and antimicrobial susceptibility testing...
May 4, 2016: Infection
Abdoul-Salam Ouédraogo, Soufiane Sanou, Aimée Kissou, Armel Poda, Salim Aberkane, Nicolas Bouzinbi, Boubacar Nacro, Rasmata Ouédraogo, Philippe Van De Perre, Christian Carriere, Dominique Decré, Hélène Jean-Pierre, Sylvain Godreuil
Extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been described worldwide, but few reports focused on Burkina Faso. To assess the prevalence of digestive carriage of such bacteria in the community and in the hospital, 214 fecal samples, 101 from healthy volunteers and 113 from hospitalized patients without digestive pathology, were collected in Bobo Dioulasso, Burkina Faso economic capital, during July and August 2014. Stool samples were screened using ESBL agar plates. Strains were identified by mass spectrometry using the Biotyper MALDI-TOF...
April 19, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
J Veenemans, M Welker, A van Belkum, M C Saccomani, V Girard, A Pettersson, C Verhulst, M Kluytmans-Vandenbergh, J Kluytmans
Typing of bacterial isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) potentially provides an efficient on-site method to monitor the spread of antibiotic-resistant bacteria and rapidly detect outbreaks. We compared MALDI-MS typing results to those of amplified fragment length polymorphism (AFLP) in a collection of 52 ESBL-producing Escherichia coli, isolated in a Dutch nursing home with an on-going outbreak of ST131 E. coli. Specific MALDI types were defined based on spectral data from four replicate colony samples of isolates grown on Columbia agar using multivariate statistical procedures...
May 2016: European Journal of Clinical Microbiology & Infectious Diseases
Alper Karagöz, Sümeyra Acar, Hanifi Körkoca
BACKGROUND/AIM: The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITEK 2 with the routine antimicrobial susceptibility testing method. MATERIALS AND METHODS: This study tested 51 Klebsiella spp...
2015: Turkish Journal of Medical Sciences
Peter Damborg, Malene Kjelin Morsing, Tanja Petersen, Valeria Bortolaia, Luca Guardabassi
BACKGROUND: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are increasingly reported in dogs. The objective of this study was to provide data on the prevalence of ESBL-producing E. coli in dog faecal deposits in public gardens. RESULTS: A total of 209 faecal deposits collected in nine public gardens in Copenhagen, Denmark were screened by selective enrichment followed by plating on MacConkey agar supplemented with cefotaxime. Presumptive ESBL-producing E...
November 25, 2015: Acta Veterinaria Scandinavica
James A Karlowsky, Douglas J Biedenbach, Samuel K Bouchillon, Joseph P Iaconis, Edina Reiszner, Daniel F Sahm
OBJECTIVES: The objective of this study was to analyse antimicrobial susceptibility testing data generated by the Assessing Worldwide Antimicrobial Resistance Evaluation (AWARE) global surveillance programme for pathogens causing skin and soft tissue infections (SSTIs) in European countries in 2012. METHODS: Confirmation of pathogen identity by MALDI-TOF and antimicrobial susceptibility testing following the CLSI broth microdilution method were performed by a central laboratory...
January 2016: Journal of Antimicrobial Chemotherapy
Anne Beyer, Sven Baumann, Gesine Scherz, Jessica Stahl, Martin von Bergen, Anika Friese, Uwe Roesler, Manfred Kietzmann, Walther Honscha
BACKGROUND: Healthy farm animals have been found to act as a reservoir of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli). Therefore, the objective of the study was to determine the input of antimicrobial active ceftiofur metabolites in the stable via faeces and urine after intramuscular administration of the drug to pigs and the elucidation of the Escherichia coli ESBL resistance pattern of treated and untreated pigs housed in the same barn during therapy. METHODS: For determination of the minimal inhibitory concentration (MIC) the method of microdilutionaccording to the recommended procedure of the Clinical and Laboratory Standards Institute was used...
2015: BMC Veterinary Research
Mette Marie Rasmussen, Japheth A Opintan, Niels Frimodt-Møller, Bjarne Styrishave
The use of antibiotics in food animals is of public health concern, because resistant zoonotic pathogens can be transmitted to humans. Furthermore, global trade with food may rapidly spread multi-resistant pathogens between countries and even continents. The purpose of the study was to investigate whether imported chicken meat and meat from locally reared chicken are potential sources for human exposure to multi resistant Escherichia coli isolates. 188 samples from imported and locally produced chicken meat were sampled and analyzed...
2015: PloS One
Tatiana Čornejová, Jan Venglovsky, Gabriela Gregova, Marta Kmetova, Vladimir Kmet
INTRODUCTION AND OBJECTIVE: Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coli and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated from an urban wastewater treatment plant. MATERIALS AND METHOD: Wastewater samples and artificially added polystyrene pellets were used as the source for E...
2015: Annals of Agricultural and Environmental Medicine: AAEM
H M Abdallah, E A Reuland, B B Wintermans, N Al Naiemi, A Koek, A M Abdelwahab, A M Ammar, A A Mohamed, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of the present study was to determine the prevalence and to characterize extended-spectrum β-lactamases- and/or carbapenemases-producing Enterobacteriaceae among Enterobacteriaceae isolated from retail chicken meat in Zagazig, Egypt. METHODS: One hundred and six Enterobacteriaceae isolates were collected from retail chicken meat samples purchased in Zagazig, Egypt in 2013. Species identification was done by MALDI-TOF MS. Screening for ESBL-E was performed by inoculation of isolates recovered from meat samples onto the EbSA (Cepheid Benelux, Apeldoorn, the Netherlands) selective screening agar...
2015: PloS One
Nour Chems el Houda Khennouchi, Lotfi Loucif, Nafissa Boutefnouchet, Hamoudi Allag, Jean-Marc Rolain
Enterobacter cloacae is among the most important pathogens responsible for nosocomial infections and outbreaks. In this study, 77 Enterobacter isolates were collected: 27 isolates from Algerian hospitals (in Constantine, Annaba, and Skikda) and 50 isolates from Marseille, France. All strains were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Antibiotic susceptibility testing was performed by the disk diffusion method. PCR was used to detect extended-spectrum-beta-lactamase (ESBL)-encoding, fluoroquinolone resistance-encoding, and aminoglycoside-modifying enzyme (AME) genes...
October 2015: Antimicrobial Agents and Chemotherapy
J Fischer, K Hille, A Mellmann, F Schaumburg, L Kreienbrock, R Köck
Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) have recently emerged in livestock and humans. Therefore, this study assessed the carriage of Enterobacteriaceae in the anterior nares and associated antimicrobial resistance in pig-exposed persons. Nasal swabs were enriched in non-selective broth and then plated on MacConkey and ESBL-selective agars. Species was confirmed by matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry (MALDI-ToF MS). Antimicrobial susceptibility testing was performed according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines...
March 2016: Epidemiology and Infection
Halima Mezhoud, Filip Boyen, Leg-Hel Touazi, An Garmyn, Nassim Moula, Annemieke Smet, Freddy Haesbrouck, An Martel, Mokrane Iguer-Ouada, Abdelaziz Touati
Extended spectrum β-lactamases (ESBL)-producing Escherichia coli have emerged worldwide in animal husbandry and they were reported from different ecosystems. The purpose of this study was firstly, to investigate the presence of ESBL-producing E. coli in the gastrointestinal (GIT) and reproductive (RT) tracts of broiler breeding roosters, and secondly to study the impact of an ESBL-producing E. coli on artificially infected semen. A total of seventeen ESBL-producing E. coli strains were isolated from the gastrointestinal and reproductive tracts of nine broiler breeding roosters...
August 2015: Animal Reproduction Science
H M Abdallah, B B Wintermans, E A Reuland, A Koek, N al Naiemi, A M Ammar, A A Mohamed, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of the study was to investigate the prevalence of extended-spectrum β-lactamase and carbapenemase production among Enterobacteriaceae isolated from Egyptian patients with suspected blood stream infection. METHODS: Ninety-four Enterobacteriaceae blood culture isolates from Egyptian patients with suspected blood stream infection were collected, one isolate per patient. Identification of bacterial isolates was performed with MALDI-TOF (MS-based Vitek MS system, bioMerieux)...
2015: PloS One
Camille Lasserre, Luc De Saint Martin, Gaelle Cuzon, Pierre Bogaerts, Estelle Lamar, Youri Glupczynski, Thierry Naas, Didier Tandé
The recognition of carbapenemase-producing Enterobacteriaceae (CPE) isolates is a major laboratory challenge, and their inappropriate or delayed detection may have negative impacts on patient management and on the implementation of infection control measures. We describe here a matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based method to detect carbapenemase activity in Enterobacteriaceae. After a 20-min incubation of the isolate with 0.5 mg/ml imipenem at 37°C, supernatants were analyzed by MALDI-TOF in order to identify peaks corresponding to imipenem (300 Da) and an imipenem metabolite (254 Da)...
July 2015: Journal of Clinical Microbiology
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