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https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#1
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28506272/prevalence-and-clonal-relationship-of-esbl-producing-salmonella-strains-from-humans-and-poultry-in-northeastern-algeria
#2
Samia Djeffal, Sofiane Bakour, Bakir Mamache, Rachid Elgroud, Amir Agabou, Selma Chabou, Sana Hireche, Omar Bouaziz, Kheira Rahal, Jean-Marc Rolain
BACKGROUND: The aims of this study were to investigate Salmonella contamination in broiler chicken farms and slaughterhouses, to assess the antibiotic resistance profile in avian and human Salmonella isolates, and to evaluate the relationship between avian and human Extended Spectrum β-Lactamase (ESBL)-producing isolates. Salmonella was screened in different sample matrices collected at thirty-two chicken farms and five slaughterhouses. The human isolates were recovered from clinical specimens at the University Teaching Hospital of Constantine (UTH)...
May 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28486169/characterization-of-oxa-48-like-producing-enterobacteriaceae-isolated-from-river-water-in-algeria
#3
Rima Tafoukt, Abdelaziz Touati, Thongpan Leangapichart, Sofiane Bakour, Jean-Marc Rolain
The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
April 30, 2017: Water Research
https://www.readbyqxmd.com/read/28467251/a-first-insight-into-escherichia-coli-st131-high-risk-clone-among-extended-spectrum-beta-lactamase-producing-urine-isolates-in-istanbul-with-the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-real-time-pcr
#4
Elif Aktaş, Barış Otlu, Duygu Erdemir, Hatice Ekici, Emin Bulut
OBJECTIVES: We aim to investigate, as a first insight, the presence and rates of high-risk Escherichia coli ST131 clone in Istanbul and evaluate antimicrobial resistance and CTX-M-15 production of ST131 and non-ST131 isolates. The use of MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry) to detect E. coli ST131 clone is also evaluated. MATERIALS AND METHODS: A total of 203 extended-spectrum beta-lactamase (ESBL)-producing urinary isolates from a training hospital in Istanbul were investigated...
May 3, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28444315/towards-the-early-detection-of-%C3%AE-lactamase-producing-enterobacteriaceae-by-maldi-tof-ms-analysis
#5
Marina Oviaño, Marta Gómara, Maria José Barba, María José Revillo, Luis Pedro Barbeyto, Germán Bou
Objectives: Development of a novel MALDI-TOF MS-based method for the rapid detection of ESBL-producing Enterobacteriaceae. Methods: The method was evaluated in terms of sensitivity, specificity and turnaround time regarding the antibiotic used (cefotaxime, ceftazidime, ceftriaxone, cefpodoxime or cefepime) and the performance of the automated MBT STAR-BL software (Bruker Daltonik GmbH, Germany) relative to qualitative interpretation of spectra for detecting β-lactamase resistance by MALDI-TOF MS (Bruker Daltonik) in a collection of 11 isogenic Escherichia coli control strains expressing different β-lactamases...
April 24, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28408060/bacterial-epidemiology-and-antimicrobial-resistance-profiles-of-urinary-specimens-of-the-elderly
#6
F Gravey, G Loggia, A de La Blanchardière, V Cattoir
OBJECTIVE: Although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. Our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. METHODS: From October 2012 to October 2015, all urinary specimens (catheter-related or not) received at the laboratory of microbiology of the university hospital of Caen (France) were retrospectively studied...
April 10, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28018582/carriage-of-esbl-ampc-producing-or-ciprofloxacin-non-susceptible-escherichia-coli-and-klebsiella-spp-in-healthy-people-in-norway
#7
Charlotte R Ulstad, Margrete Solheim, Sophie Berg, Morten Lindbæk, Ulf R Dahle, Astrid L Wester
BACKGROUND: Asymptomatic carriage has been recognised as an important risk factor for infection caused by antibiotic resistant bacteria. A 14% global prevalence of Extended-Spectrum Beta-lactamase (ESBL) carriage was recently reported, but large intra-and interregional variations were observed. We investigated the faecal carriage rates of ESBL-, AmpC-producing and ciprofloxacin non-susceptible Escherichia coli and Klebsiella spp. in healthy Norwegians. METHODS: Rectal samples were obtained from 284 volunteers, together with demographic data and information on recent travel history...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27984780/high-rates-of-ctx-m-15-producing-escherichia-coli-and-klebsiella-pneumoniae-in-wild-boars-and-barbary-macaques-in-algeria
#8
Taous Bachiri, Sofiane Bakour, Rabia Ladjouzi, Leangapichart Thongpan, Jean Marc Rolain, Abdelaziz Touati
OBJECTIVES: The present study aimed to screen for the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in wild boars and Barbary macaques in Béjaïa and Jijel, Algeria. METHODS: A total of 216 faecal samples collected between September 2014 and August 2015 were cultured on MacConkey agar supplemented with 1μg/mL ceftazidime. Isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS)...
December 10, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#9
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27821357/evaluation-of-meat-fruit-and-vegetables-from-retail-stores-in-five-united-kingdom-regions-as-sources-of-extended-spectrum-beta-lactamase-esbl-producing-and-carbapenem-resistant-escherichia-coli
#10
L P Randall, M P Lodge, N C Elviss, F L Lemma, K L Hopkins, C J Teale, N Woodford
We determined the prevalence and types of extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Escherichia coli in raw retail beef, chicken, pork, fruit and vegetables in five UK regions in 2013-14. Raw meat (n=397), and fruit and vegetable samples (n=400) were purchased from retail stores in London, East Anglia, North West England, Scotland and Wales. Samples were tested for the presence of ESBL-producing E. coli by plating enriched samples on CHROMagar CTX and CHROMagar ESBL, for AmpC-type E...
January 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27801367/prevalence-of-esbl-producing-enterobacteriaceae-isolated-from-blood-cultures-in-mali
#11
Samba Adama Sangare, Almoustapha Issiaka Maiga, Ibrehima Guindo, Aminata Maiga, Namory Camara, Oumar Agaly Dicko, Souleymane Diallo, Flabou Bougoudogo, Laurence Armand-Lefevre, Antoine Andremont, Ibrahim Izetiegouma Maiga
INTRODUCTION: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali. METHODOLOGY: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated...
October 31, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27794265/molecular-epidemiology-of-environmental-and-clinical-carbapenemase-producing-gram-negative-bacilli-from-hospitals-in-guelma-algeria-multiple-genetic-lineages-and-first-report-of-oxa-48-in-enterobacter-cloacae
#12
Widad Bouguenoun, Sofiane Bakour, Ahmed Aimen Bentorki, Charbel Al Bayssari, Tarek Merad, Jean-Marc Rolain
This study was designed to investigate environmental colonisation in Algerian hospitals by carbapenem-resistant Gram-negative bacilli (GNB), including molecular characterisation of their resistance, and to perform a comparative molecular analysis between clinical and environmental strains. GNB isolated from hospitalised patients and the hospital environment were identified using microbiological methods and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed by disk diffusion and Etest methods...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27598775/low-enteric-colonization-with-multidrug-resistant-pathogens-in-soldiers-returning-from-deployments-experience-from-the-years-2007-2015
#13
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
https://www.readbyqxmd.com/read/27530851/molecular-characterisation-of-extended-spectrum-%C3%AE-lactamase-and-plasmid-ampc-producing-escherichia-coli-strains-isolated-from-broilers-in-b%C3%A3-ja%C3%A3-a-algeria
#14
Mohamed Belmahdi, Sofiane Bakour, Charbel Al Bayssari, Abdelaziz Touati, Jean-Marc Rolain
This study aimed to characterise the molecular support of antibiotic resistance in expanded-spectrum cephalosporin (ESC)-resistant Escherichia coli isolates recovered from healthy broilers in Béjaïa, northeast Algeria. A total of 61 intestinal swabs from slaughtered broilers from four regions in Béjaïa locality, Algeria, were collected between February and April 2014, from which 20 ESC-resistant E. coli strains were isolated. Escherichia coli isolates were identified by classical biochemical and MALDI-TOF methods...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27461798/in-vitro-activity-of-ceftaroline-against-bacterial-pathogens-isolated-from-patients-with-skin-and-soft-tissue-and-respiratory-tract-infections-in-african-and-middle-eastern-countries-aware-global-surveillance-program-2012-2014
#15
James A Karlowsky, Douglas J Biedenbach, Samuel K Bouchillon, Meredith Hackel, Joseph P Iaconis, Daniel F Sahm
The objective of this report was to document antimicrobial susceptibility testing surveillance data for ceftaroline and comparative agents from the AWARE (Assessing Worldwide Antimicrobial Resistance Evaluation) global surveillance program for bacterial pathogens causing skin and soft tissue and respiratory tract infections in African and Middle Eastern countries from 2012 through 2014. Pathogen identities were confirmed by MALDI-TOF and antimicrobial susceptibility testing performed by CLSI broth microdilution methodology in a central laboratory...
October 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27400864/high-prevalence-of-extended-spectrum-%C3%A3-lactamase-producing-enterobacteriaceae-among-clinical-isolates-in-burkina-faso
#16
Abdoul-Salam Ouedraogo, Mahamadou Sanou, Aimée Kissou, Soufiane Sanou, Hermann Solaré, Firmin Kaboré, Armel Poda, Salim Aberkane, Nicolas Bouzinbi, Idrissa Sano, Boubacar Nacro, Lassana Sangaré, Christian Carrière, Dominique Decré, Rasmata Ouégraogo, Hélène Jean-Pierre, Sylvain Godreuil
BACKGROUND: Nothing is known about the epidemiology and resistance mechanisms of extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-PE) in Burkina Faso. The objective of this study was to determine ESBL-PE prevalence and to characterize ESBL genes in Burkina Faso. METHODS: During 2 months (June-July 2014), 1602 clinical samples were sent for bacteriologic investigations to the microbiology laboratories of the tree main hospitals of Burkina Faso. Isolates were identified by mass spectrometry using a matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) BioTyper...
July 11, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27171497/epidemiology-and-resistance-patterns-of-bacterial-and-fungal-colonization-of-biliary-plastic-stents-a-prospective-cohort-study
#17
Christoph Lübbert, Karolin Wendt, Jürgen Feisthammel, Annette Moter, Norman Lippmann, Thilo Busch, Joachim Mössner, Albrecht Hoffmeister, Arne C Rodloff
BACKGROUND: Plastic stents used for the treatment of biliary obstruction will become occluded over time due to microbial colonization and formation of biofilms. Treatment of stent-associated cholangitis is often not effective because of inappropriate use of antimicrobial agents or antimicrobial resistance. We aimed to assess the current bacterial and fungal etiology of stent-associated biofilms, with particular emphasis on antimicrobial resistance. METHODS: Patients with biliary strictures requiring endoscopic stent placement were prospectively enrolled...
2016: PloS One
https://www.readbyqxmd.com/read/27147419/a-rare-cause-of-infection-raoultella-planticola-emerging-threat-and-new-reservoir-for-carbapenem-resistance
#18
Tayfur Demiray, Mehmet Koroglu, Ahmet Ozbek, Mustafa Altindis
BACKGROUND: Severe cases of infections caused by Raoultella planticola are constantly being reported from all over the world with the increase in drug-resistance patterns. In this study, we retrospectively evaluated the clinical characteristics of R. planticola infections with patients' demographics and antimicrobial susceptibilities of the R. planticola isolates. METHODS: R. planticola isolates were retrospectively evaluated. VITEK 2(®) automated system was used for identification and antimicrobial susceptibility testing...
December 2016: Infection
https://www.readbyqxmd.com/read/27092971/fecal-carriage-of-enterobacteriaceae-producing-extended-spectrum-beta-lactamases-in-hospitalized-patients-and-healthy-community-volunteers-in-burkina-faso
#19
Abdoul-Salam Ouédraogo, Soufiane Sanou, Aimée Kissou, Armel Poda, Salim Aberkane, Nicolas Bouzinbi, Boubacar Nacro, Rasmata Ouédraogo, Philippe Van De Perre, Christian Carriere, Dominique Decré, Hélène Jean-Pierre, Sylvain Godreuil
Extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) have been described worldwide, but few reports focused on Burkina Faso. To assess the prevalence of digestive carriage of such bacteria in the community and in the hospital, 214 fecal samples, 101 from healthy volunteers and 113 from hospitalized patients without digestive pathology, were collected in Bobo Dioulasso, Burkina Faso economic capital, during July and August 2014. Stool samples were screened using ESBL agar plates. Strains were identified by mass spectrometry using the Biotyper MALDI-TOF...
January 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/26922068/comparison-of-maldi-tof-ms-and-aflp-for-strain-typing-of-esbl-producing-escherichia-coli
#20
J Veenemans, M Welker, A van Belkum, M C Saccomani, V Girard, A Pettersson, C Verhulst, M Kluytmans-Vandenbergh, J Kluytmans
Typing of bacterial isolates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) potentially provides an efficient on-site method to monitor the spread of antibiotic-resistant bacteria and rapidly detect outbreaks. We compared MALDI-MS typing results to those of amplified fragment length polymorphism (AFLP) in a collection of 52 ESBL-producing Escherichia coli, isolated in a Dutch nursing home with an on-going outbreak of ST131 E. coli. Specific MALDI types were defined based on spectral data from four replicate colony samples of isolates grown on Columbia agar using multivariate statistical procedures...
May 2016: European Journal of Clinical Microbiology & Infectious Diseases
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