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https://www.readbyqxmd.com/read/27889877/maldi-tof-ms-meets-wgs-in-a-vre-outbreak-investigation
#1
S Schlebusch, G R Price, R L Gallagher, V Horton-Szar, L D H Elbourne, P Griffin, D J Venter, S O Jensen, S J Van Hal
The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization-time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology-oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks...
November 26, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27598775/low-enteric-colonization-with-multidrug-resistant-pathogens-in-soldiers-returning-from-deployments-experience-from-the-years-2007-2015
#2
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
https://www.readbyqxmd.com/read/27156245/characterization-and-rapid-control-of-a-vancomycin-resistant-enterococcus-faecium-vref-outbreak-in-a-renal-transplant-unit-in-spain-the-environment-matters
#3
Sabina Herrera, Luisa Sorlí, Maria Jose Pérez-Sáez, Patricia Ruiz-Garbajosa, Clara Barrios, Virginia Plasencia, Milagro Montero, Roser Terradas, Marta Crespo, Xavier Castells, Rafael Cantón, Julio Pascual, Juan Pablo Horcajada
OBJECTIVE: To describe a clonal outbreak due to vancomycin-resistant Enterococcus faecium (VREF) in the nephrology and renal transplant unit of a tertiary teaching hospital in Barcelona, Spain, and to highlight how active patient and environment surveillance cultures, as well as prompt and directed intervention strategies, mainly environmental, helped to successfully bring it under control. PATIENTS AND METHODS: A study was conducted on patients admitted to the nephrology ward with any culture positive for VREF over a 6-month period (August 2012-January 2013)...
May 4, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/26357893/studies-on-the-drug-resistance-profile-of-enterococcus-faecium-distributed-from-poultry-retailers-to-hospitals
#4
Alya Limayem, Robert Scott Donofrio, Chao Zhang, Edward Haller, Michael G Johnson
The multidrug resistant Enterococcus faecium (MEF) strains originating from farm animals are proliferating at a substantial pace to impact downstream food chains and could reach hospitals. This study was conducted to elucidate the drug susceptibility profile of MEF strains collected from poultry products in Ann Arbor, MI area and clinical settings from Michigan State Lab and Moffitt Cancer Center (MCC) in Florida. Presumptive positive Enterococcus isolates at species level were identified by Matrix Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) analysis...
2015: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/25905870/application-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-the-screening-of-vana-positive-enterococcus-faecium
#5
Li-jun Wang, Xin-xin Lu, Wei Wu, Wen-jun Sui, Gui Zhang
In order to evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MAIDI-TOF MS) assay in screening vancomycin-resistant Enterococcus faecium, a total of 150 E. faecium clinical strains were studied, including 60 vancomycin-resistant E. faecium (VREF) isolates and 90 vancomycin-susceptible (VSEF) strains. Vancomycin resistance genes were detected by sequencing. E. faecium were identified by MALDI-TOF MS. A genetic algorithm model with ClinProTools software was generated using spectra of 30 VREF isolates and 30 VSEF isolates...
2014: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/25336164/complementary-use-of-maldi-tof-ms-and-real-time-pcr-melt-curve-analysis-for-rapid-identification-of-methicillin-resistant-staphylococci-and-vre
#6
Wai-Sing Chan, Tsz-Ming Chan, Tsz-Wan Lai, Jasper Fuk-Woo Chan, Raymond Wai-Man Lai, Christopher Koon-Chi Lai, Bone Siu-Fai Tang
OBJECTIVES: To develop a rapid method for routine screening of methicillin-resistant staphylococci and VRE for clinical isolates and positive blood cultures. METHODS: Our method consisted of two parts: MALDI-TOF MS was used for identification of staphylococci and enterococci, followed by antibiotic resistance detection by real-time PCR-melt curve analysis without DNA extraction. The latter part included a triplex reaction for staphylococcal culture isolates (mecA, mecALGA251 and Panton-Valentine leucocidin genes), dual PCR of mecA/mecALGA251 and nuc genes for staphylococcal blood cultures, and a duplex reaction for enterococci (vanA and vanB genes)...
February 2015: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/25084643/isolation-and-characterization-of-multidrug-resistant-bacteria-from-minced-meat-in-austria
#7
Christian Petternel, Herbert Galler, Gernot Zarfel, Josefa Luxner, Doris Haas, Andrea J Grisold, Franz F Reinthaler, Gebhard Feierl
INTRODUCTION: Resistant bacteria are a well-known public health problem. This study was conducted to investigate the prevalence and genetic characteristics of extended spectrum β-lactamase (ESBL) producing enterobacteria, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) in mixed minced meat from pork and beef. METHODS: One hundred samples of mixed minced meat were collected from supermarkets (n = 70) and local butcher shops (n = 30) in the city of Graz (Austria)...
December 2014: Food Microbiology
https://www.readbyqxmd.com/read/24048531/evaluation-of-the-nanosphere-verigene-gram-positive-blood-culture-assay-with-the-versatrek-blood-culture-system-and-assessment-of-possible-impact-on-selected-patients
#8
COMPARATIVE STUDY
Stacy G Beal, Jane Ciurca, Geremy Smith, Jeffrey John, Francesca Lee, Christopher D Doern, Rita M Gander
The Verigene Gram-positive blood culture (BC-GP) assay (Nanosphere, Northbrook, IL) is a molecular method for the rapid identification of Gram-positive organisms and resistance markers directly from blood culture bottles. A total of 148 VersaTREK REDOX 1 40-ml aerobic bottles demonstrating Gram-positive bacteria were tested. Results were compared with those from conventional biochemical and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identifications. We obtained isolates of methicillin-resistant Staphylococcus aureus (MRSA) (24), methicillin-susceptible Staphylococcus aureus (MSSA) (14), methicillin-resistant Staphylococcus epidermidis (MRSE) (17), methicillin-susceptible Staphylococcus epidermidis (MSSE) (9), other coagulase-negative staphylococci (19), Streptococcus salivarius (5), Streptococcus parasanguinis (2), Streptococcus sanguinis (1), Streptococcus cristatus (1), the Streptococcus bovis group (5), Streptococcus agalactiae (9), the Streptococcus anginosus group (1), Streptococcus pneumoniae (6), vancomycin-resistant Enterococcus faecium (VRE FCM) (16), vancomycin-susceptible Enterococcus faecalis (3), Aerococcus viridans (2), Bacillus (6), Corynebacterium (8), Lactobacillus (2), Micrococcus (2), Neisseria mucosa (1), Escherichia coli (3), Candida tropicalis (1), Propionibacterium (1), and Rothia (1)...
December 2013: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/23996387/lysozyme-encapsulated-gold-nanocluster-based-affinity-mass-spectrometry-for-pathogenic-bacteria
#9
Po-Han Chan, Song-Yi Wong, Shu-Hsuan Lin, Yu-Chie Chen
RATIONALE: Bacterial infections can be difficult to treat and can lead to irreversible damage to patients if proper treatment is not provided in time. Additionally, the emerging threat from antibiotic-resistant bacterial strains makes medical treatment even more difficult. Thus, rapid identification of infected bacterial strains is essential to assist diagnostics and medical treatment. METHODS: Lysozymes are glycoside hydrolases that can bind with peptidoglycans on bacterial cell walls...
October 15, 2013: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/22740710/use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-to-identify-vancomycin-resistant-enterococci-and-investigate-the-epidemiology-of-an-outbreak
#10
Paul M Griffin, Gareth R Price, Jacqueline M Schooneveldt, Sanmarié Schlebusch, Martyn H Tilse, Tess Urbanski, Brett Hamilton, Deon Venter
The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables and highly trained staff. An accurate, rapid diagnostic test has the ability to greatly reduce the spread of this organism, which has the ability to colonize patients for long periods, potentially even lifelong...
September 2012: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/21999249/novel-conjugation-of-norvancomycin-fluorescein-for-photodynamic-inactivation-of-bacillus-subtilis
#11
Hui-Zhou Gao, Ke-Wu Yang, Xiang-Long Wu, Jia-Yun Liu, Lei Feng, Jian-Min Xiao, Li-Sheng Zhou, Chao Jia, Zhen Shi
A simple and unique conjugation of norvancomycin-fluorescein (VanF) has been achieved. It was characterized by UV-vis and fluorescence spectra and confirmed by MALDI-TOF mass spectrum. The photodynamic assay indicated that VanF effectively inactivated the Gram-positive Bacillus subtilis (ATCC 6633) from clinic with inactivation rate of 30-70% within 1-7.5 μM. In vitro, VanF showed low antimicrobial activity with value of >128 μg/mL, binding affinity with value of 180 nM per 10(8) cells/mL against the bacteria strains...
November 16, 2011: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/20858227/proteomic-characterization-of-vana-containing-enterococcus-recovered-from-seagulls-at-the-berlengas-natural-reserve-w-portugal
#12
Hajer Radhouani, Patrícia Poeta, Luís Pinto, Júlio Miranda, Céline Coelho, Carlos Carvalho, Jorge Rodrigues, María López, Carmen Torres, Rui Vitorino, Pedro Domingues, Gilberto Igrejas
BACKGROUND: Enterococci have emerged as the third most common cause of nosocomial infections, requiring bactericidal antimicrobial therapy. Although vancomycin resistance is a major problem in clinics and has emerged in an important extend in farm animals, few studies have examined it in wild animals. To determine the prevalence of vanA-containing Enterococcus strains among faecal samples of Seagulls (Larus cachinnans) of Berlengas Natural Reserve of Portugal, we developed a proteomic approach integrated with genomic data...
2010: Proteome Science
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