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https://www.readbyqxmd.com/read/28719803/elevated-sterol-regulatory-elementary-binding-protein-1-and-glua2-levels-in-the-hippocampal-nuclear-fraction-of-genetic-absence-epilepsy-rats-from-strasbourg
#1
Sathiya Sekar, Entesar Omran, Venkat Gopalakrishnan, John G Howland, Terrance P Snutch, Changiz Taghibiglou
Studies in animal models and human tissues show that nuclear translocation of sterol regulatory element binding protein 1 (SREBP1) and glutamate A2 subunit (GluA2) of cell-surface AMPA receptor (AMPAR) trigger neuronal excitotoxicity-induced apoptosis in stroke. However, it is not known whether a similar type of underlying pathophysiology occurs in absence epilepsy. To explore this issue, we examined the levels of mature SREBP1, GluA2, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), p53, and activated to total caspase 3 ratio in nuclear fractions (NF) of hippocampal homogenate from 8 to 10 week old male Genetic Absence Epilepsy Rats from Strasbourg (GAERS) and non-epileptic control (NEC) strains...
July 12, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/28719345/h2o2-induced-mild-stress-in-relation-with-in-vitro-ovine-oocyte-developmental-competence-implications-for-blastocyst-apoptosis-and-related-genes-expression
#2
K Nikdel, M Aminafshar, A Mohammadi-Sangcheshmeh, N EmamJomeh-Kashan, E Seyedjafari
In this study, in vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 µM) of H2O2. The intracellular glutathione (GSH) level, fertilization, cleavage, and blastocyst rates, total cell number, and apoptotic cell number and expression of Bax, Bcl-2, and p53 genes in blastocyst-stage embryos were studied. At 10 μM H2O2 concentration, a higher GSH level was detected in comparison to the other groups while oocytes exposed to 1000 μM H2O2 had the lowest GSH level. Treatment of oocytes with 1000 μM H2O2 decreased the rate of two pronuclei formation as compared with other groups...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28718916/the-genetic-landscape-of-endometrial-clear-cell-carcinomas
#3
Deborah F DeLair, Kathleen A Burke, Pier Selenica, Raymond S Lim, Sasinya N Scott, Sumit Middha, Abhinita S Mohanty, Donavan T Cheng, Michael F Berger, Robert A Soslow, Britta Weigelt
Clear cell carcinoma of the endometrium is a rare type of endometrial cancer generally associated with an aggressive clinical behavior. Here we sought to define the repertoire of somatic genetic alterations in endometrial clear cell carcinomas (ECCs) and whether ECCs could be classified into the molecular subtypes described for endometrial endometrioid and serous carcinomas. We performed a rigorous histopathological review, immunohistochemical analysis and massively parallel sequencing targeting 300 cancer-related genes of 32 pure ECCs...
July 18, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28718726/effects-of-the-notch1-signaling-pathway-on-human-lung-cancer-a549-cells
#4
Yun Zeng, Bijian Yin, Xinwei Wang, Guohao Xia, Zhengjie Shen, Wenzhe Gu, Mianhua Wu
PURPOSE: To evaluate the effects of the Notch1 signaling pathway on human lung cancer A549 cells. MATERIALS AND METHODS: A549 cells were transfected with recombinant plasmids. Cell proliferation was detected by MTT assay. A tumor-bearing mouse model was established for intratumoral gene injection. Apoptosis-related factors were detected by immunohistochemical assay. Caspase-8, caspase-3, caspase-9, PI3K, pAkt and pSTAT3 expressions were detected by Western blotting...
July 18, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/28718722/anticarcinogenic-effects-of-dried-citrus-peel-in-colon-carcinogenesis-due-to-inhibition-of-oxidative-stress
#5
Wakana Onuma, Daichi Asai, Susumu Tomono, Shingo Miyamoto, Gen Fujii, Takahiro Hamoya, Aya Nagano, Satoru Takahashi, Shoji Masumori, Noriyuki Miyoshi, Keiji Wakabayashi, Michihiro Mutoh
Colorectal cancer is one of the leading causes of death worldwide. Reactive oxygen species produce oxidative stress and contribute to colorectal carcinogenesis. Because dietary citrus has been shown to reduce oxidative stress, we investigated the effects of citrus peel extract at dilutions of 1/200-1/500 on the activity of oxidative-stress-related transcription factors, including AP-1, NF-κB, NRF2, p53, and STAT3, in human colon cancer cell line HCT116 cells using a luciferase reporter gene assay. NRF2 transcriptional activities were 1...
July 18, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28718668/olea-europaea-leaf-extract-improves-the-efficacy-of-temozolomide-therapy-by-inducing-mgmt-methylation-and-reducing-p53-expression-un-glioblastoma
#6
Gulcin Tezcan, Berrin Tunca, Hilal Demirci, Ahmet Bekar, Mevlut Ozgur Taskapilioglu, Hasan Kocaeli, Unal Egeli, Gulsah Cecener, Sahsine Tolunay, Ozgur Vatan
Unmethylated O6-methylguanine-DNA-methyltransferase (MGMT) promoter leads to Temozolomide (TMZ) resistance in most of the glioblastoma multiforme (GBM) patients. We previously investigated the synergistic effect of Olea europaea leaf extract (OLE) on TMZ cytotoxicity through modulating microRNA expression. To date, knowledge about the effect of OLE on MGMT methylation is insufficient. The aim of the current study was to evaluate the potential modulating effect of OLE on the TMZ response of GBM tumors through MGMT methylation...
July 18, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28718400/histone-h3g34r-mutation-causes-replication-stress-homologous-recombination-defects-and-genomic-instability-in-s-pombe
#7
Rajesh K Yadav, Carolyn M Jablonowski, Alfonso G Fernandez, Brandon R Lowe, Ryan A Henry, David Finkelstein, Kevin J Barnum, Alison L Pidoux, Yin-Ming Kuo, Jie Huang, Matthew J O'Connell, Andrew J Andrews, Arzu Onar-Thomas, Robin C Allshire, Janet F Partridge
Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express only the mutant histone H3. H3-G34R specifically reduces H3K36 tri-methylation and H3K36 acetylation, and mutants show partial transcriptional overlap with set2 deletions. H3-G34R mutants exhibit genomic instability and increased replication stress, including slowed replication fork restart, although DNA replication checkpoints are functional...
July 18, 2017: ELife
https://www.readbyqxmd.com/read/28718371/changes-in-the-expression-of-genes-involved-in-cell-cycle-regulation-and-the-relative-telomere-length-in-the-process-of-canceration-induced-by-omethoate
#8
Xiaoran Duan, Yongli Yang, Sihua Wang, Xiaolei Feng, Tuanwei Wang, Pengpeng Wang, Suxiang Liu, Lei Li, Wu Yao, Liuxin Cui, Wei Wang
Organophosphorous pesticides (OPs), with high efficiency, broad-spectrum and low residue, are widely used in China. Omethoate is a broad category of organophosphorous pesticides and is more domestically utilized which has chronic toxic effect on human health caused by long-term, low-dose exposure to Ops, recently its potential genotoxicity has attracted wide attention which can cause chromosomal DNA damage. Thus, the aim of this study is screen susceptible biomarkers and explore the mechanism of canceration induced by omethoate...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28718286/functionalized-selenium-nanosystem-as-radiation-sensitizer-of-125i-seeds-for-precise-cancer-therapy
#9
Yahui Yang, Qiang Xie, Zhennan Zhao, Lizhen He, Leung Chan, Yingxiang Liu, Yongle Chen, Mingjun Bai, Tao Pan, Yanni Qu, Long Ling, Tianfeng Chen
Although radiotherapy has been extensively applied in cancer treatment, external beam radiation therapy is still difficult to avoid damage to adjacent normal tissues in the process of delivering a sufficient radiation dose to the tumor sites of patients. To overcome this limitation, chemoradiotherapy, as a combination of chemotherapy and radiotherapy of radioactive seed, has been proposed to decrease the damage for tumor surrounding tissues and enhance the radiosensitivity of solid tumors. In the study, we designed and synthesized folic acid-conjugated selenium nanoparticles (FA@SeNPs) as cancer-targeting agent, which could be synergistically enhanced by the radioactive 125I seeds to realize anticancer efficacy and inhibited colony formation ability...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28718283/disordered-linker-in-p53-participates-in-non-specific-binding-to-and-1d-sliding-along-dna-revealed-by-single-molecule-fluorescence-measurements
#10
Dwiky Rendra Graha Subekti, Agato Murata, Yuji Itoh, Satoshi Fukuchi, Hiroto Takahashi, Saori Kanbayashi, Satoshi Takahashi, Kiyoto Kamagata
The tumor suppressor p53 is a multidomain transcription factor that can quickly bind to its target DNA by sliding along the DNA strand. We hypothesized that the intrinsically disordered and positively charged linker of p53 regulates its search dynamics first by directly interacting with DNA and second by modulating hopping of the core domain. To test the two hypotheses, we prepared five variants of p53 in which the length and charge of the linker were modulated. Affinity to and sliding along non-specific DNA of p53 were altered by the charge of the linker, but not by the linker length...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28716896/poor-prognosis-indicated-by-venous-circulating-tumor-cell-clusters-in-early-stage-lung-cancers
#11
Vasudha Murlidhar, Rishindra M Reddy, Shamileh Fouladdel, Lili Zhao, Martin K Ishikawa, Svetlana Grabauskiene, Zhuo Zhang, Jules Lin, Andrew C Chang, Philip W Carrott, William R Lynch, Mark B Orringer, Chandan Kumar-Sinha, Nallasivam Palanisamy, David G Beer, Max S Wicha, Nithya Ramnath, Ebrahim Azizi, Sunitha Nagrath
Early detection of metastasis can be aided by circulating tumor cells (CTCs), which also show potential to predict early relapse. Due to the limited CTC numbers in peripheral blood in early stages, we investigated CTCs in pulmonary vein blood accessed during surgical resection of tumors. Pulmonary vein (PV) and peripheral vein (Pe) blood specimens from patients with lung cancer were drawn during the perioperative period and assessed for CTC burden using a microfluidic device. From 108 blood samples analyzed from 36 patients, PV had significantly higher number of CTCs compared to pre-operative Pe (p<0...
July 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28716739/phototoxicity-in-a-laryngeal-cancer-cell-line-enhanced-by-a-targeting-amphiphilic-chlorin-photosensitizer
#12
Milene N O Moritz, Carlos Rossa, Kleber T de Oliveira, Marciana P Uliana, Janice R Perussi
BACKGROUND: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA(®)) presented a pronounced potential to induce death in HeLa cell line after PDT...
July 14, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28716725/cardamonin-attenuates-lung-carcinoma-and-promotes-autophagy-via-targeting-p53-and-regulating-mtor
#13
Shasha Hou, Xiaoyao Yin, Ziling Wang, Jinhua Zhang, Qi Yuan, Zhinan Chen
As a chalcone extracted from Alpinia rafflesiana, Cardamonin has been reported to serve as anticancer agent and induces autophagy. This study was to elucidate the effects of Cardamonin on cell proliferation and autophagy and its potential mechanism in lung carcinoma cells. In this study, CCK-8 and MTT assay were carried out to examine the impact of Cardamonin on cell multiplication in vitro. Tumor cell growth and Ki-67 (a marker of proliferation) levels were observed in a murine model; Autophagy levels were monitored by western blot, quantitative PCR and transmission electron microscope in human lung cancer cells and tumor tissues...
July 14, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28716710/microrna-143-acts-as-a-suppressor-of-hemangioma-growth-by-targeting-bcl-2
#14
Chongqing Huang, JingYong Huang, Pengyan Ma, Guanfeng Yu
Infantile hemangioma is the most common vascular tumor affecting infants, which is associated with clonal expansion of endothelial cells. The aim of this study is to determine the role of microRNA (miR)-143 in the growth and survival of hemangioma-derived endothelial cells (HemECs). We examined the expression of miR-143 in patients with proliferating-phase (n=10) and involuting-phase (n=8) hemangiomas. The effects of ectopic expression of miR-143 on the viability, proliferation, cell cycle distribution, and apoptosis of HemECs were explored...
July 14, 2017: Gene
https://www.readbyqxmd.com/read/28716577/apoptosis-related-genes-induced-in-response-to-ketamine-during-early-life-stages-of-zebrafish
#15
Luís M Félix, Cindy Serafim, Ana M Valentim, Luís M Antunes, Manuela Matos, Ana M Coimbra
Increasing evidence supports that ketamine, a widely used anaesthetic, potentiates apoptosis during development through the mitochondrial pathway of apoptosis. Defects in the apoptotic machinery can cause or contribute to the developmental abnormalities previously described in ketamine-exposed zebrafish. The involvement of the apoptotic machinery in ketamine-induced teratogenicity was addressed by assessing the apoptotic signals at 8 and 24 hpf following 20min exposure to ketamine at three stages of early zebrafish embryo development (256 cell, 50% epiboly and 1-4 somites stages)...
July 14, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28716547/cellular-senescence-regulated-by-swi-snf-complex-subunits-through-p53-p21-and-p16-prb-pathway
#16
Ling He, Ying Chen, Jianguo Feng, Weichao Sun, Shun Li, Mengting Ou, Liling Tang
SWI/SNF complex is an evolutionarily well-conserved chromatin-remodeling complex, which is implicated in the nucleosomes removing or sliding, impacting on the DNA repair, replication and genes expression regulation. The SWI/SNF complex consists up to 12 protein subunits. The catalytic subunits are BRG1 or BRM, which are exclusive ATPase subunits. BRG1 has been reported to play an important role in cellular senescence. However, The function of non-catalytic subunits involved in cellular senescence is rarely investigated...
July 14, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28716051/mir-380-5p-mediated-repression-of-tep1-and-tspyl5-interferes-with-telomerase-activity-and-favours-the-emergence-of-an-alt-like-phenotype-in-diffuse-malignant-peritoneal-mesothelioma-cells
#17
Graziella Cimino-Reale, Paolo Gandellini, Francesca Santambrogio, Marta Recagni, Nadia Zaffaroni, Marco Folini
BACKGROUND: Understanding the molecular/cellular underpinnings of diffuse malignant peritoneal mesothelioma (DMPM), a fatal malignancy with limited therapeutic options, is of utmost importance for the fruitful management of the disease. In this context, we previously found that telomerase activity (TA), which accounts for the limitless proliferative potential of cancer cells, is prognostic for disease relapse and cancer-related death in DMPM patients. Consequently, the identification of factors involved in telomerase activation/regulation may pave the way towards the development of novel therapeutic interventions for the disease...
July 17, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28716034/growth-arrest-specific-gene-2-in-tilapia-oreochromis-niloticus-molecular-characterization-and-functional-analysis-under-low-temperature-stress
#18
ChangGeng Yang, Fan Wu, Xing Lu, Ming Jiang, Wei Liu, Lijuan Yu, Juan Tian, Hua Wen
BACKGROUND: Growth arrest specific 2 (gas2) gene is a component of the microfilament system that plays a major role in the cell cycle, regulation of microfilaments, and cell morphology during apoptotic processes. However, little information is available on fish gas2. In this study, the tilapia (Oreochromis niloticus) gas2 gene was cloned and characterized for the first time. RESULTS: The open reading frame was 1020 bp, encoding 340 amino acids; the 5'-untranslated region (UTR) was 140 bp and the 3'-UTR was 70 bp, with a poly (A) tail...
July 17, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28715492/small-molecule-perturbation-of-the-cand1-cullin1-ubiquitin-cycle-stabilizes-p53-and-triggers-epstein-barr-virus-reactivation
#19
Nadezhda Tikhmyanova, Steve Tutton, Kayla A Martin, Fang Lu, Andrew V Kossenkov, Nicholas Paparoidamis, Shannon Kenney, Joseph M Salvino, Paul M Lieberman
The chemical probe C60 efficiently triggers Epstein-Barr Virus (EBV) reactivation from latency through an unknown mechanism. Here, we identify the Cullin exchange factor CAND1 as a biochemical target of C60. We also identified CAND1 in an shRNA library screen for EBV lytic reactivation. Gene expression profiling revealed that C60 activates the p53 pathway and protein analysis revealed a strong stabilization and S15 phosphorylation of p53. C60 reduced Cullin1 association with CAND1 and led to a global accumulation of ubiquitylated substrates...
July 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28715461/tlr1-10-nf-%C3%AE%C2%BAb-and-p53-expression-is-increased-in-oral-lichenoid-disease
#20
Peter Rusanen, Emilia Marttila, Johanna Uittamo, Jaana Hagström, Tuula Salo, Riina Rautemaa-Richardson
Toll-like receptors (TLRs) and nuclear factor-κB (NF-κB) in keratinocytes play an important role in dermatological autoimmune diseases. Tumour suppressor protein p53 regulates TLR expression. The aim of this study was to compare the expression of TLR1-TLR10, p53 and NF-κB in patients with oral lichenoid disease (OLD) with healthy mucosa. Oral mucosal biopsies from 24 patients with OLD and 26 healthy controls (HC) were analysed for the expression of TLR1-TLR10, NF-κB and p53 by immunohistochemistry. The expression of all TLRs was increased in OLD epithelia compared to HC samples and the difference was significant in TLR1, TLR3, TLR4, TLR5, TLR6 and TLR7...
2017: PloS One
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