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Hydrolyzed Collagen

C Kohn, J M Klemens, C Kascholke, N S Murthy, J Kohn, M Brandenburger, M C Hacker
Toward a new generation of improved nerve guidance conduits (NGCs), novel biomaterials are required to address pressing clinical shortcomings in peripheral nerve regeneration (PNR) and to promote biological performance. A dual-component hydrogel system formed by cross-linking reaction between maleic anhydride groups in an oligomeric building block for cross-linking of free amine functionalities in partially hydrolyzed collagen is formulated for continuous processing and NGC fabrication. The influence of the gelation base is optimized for processing from a double syringe delivery system with a static mixer...
October 18, 2016: Biomaterials Science
Ahmad Oryan, P Sharifi, Ali Moshiri, Ian A Silver
AIM OF THE STUDY: Large tendon defects involving extensive tissue loss present complex clinical problems. Surgical reconstruction of such injuries is normally performed by transplanting autogenous and allogenous soft tissues that are expected to remodel to mimic a normal tendon. However, the use of grafts has always been associated with significant limitations. Tissue engineering employing artificial scaffolds may provide acceptable alternatives. Gelatin is a hydrolyzed form of collagen that is bioactive, biodegradable and biocompatible...
September 23, 2016: Connective Tissue Research
Érika Lika Ida, Ronivaldo Rodrigues da Silva, Tássio Brito de Oliveira, Tatiane Beltramini Souto, Juliana Abigail Leite, André Rodrigues, Hamilton Cabral
Filamentous fungi secrete diverse peptidases with different biochemical properties, which is of considerable importance for application in various commercial sectors. In this study, we describe the isolation of two fungal species collected from the soil of decayed organic matter: Aspergillus fischeri and Penicillium citrinum. In a submerged bioprocess, we observed better peptidase production with the fungus P. citrinum, which reached a peak production at 168 h with 760 U/mL, in comparison to that with the fungus A...
August 23, 2016: Preparative Biochemistry & Biotechnology
Brant R Johnson, Todd R Klaenhammer
UNLABELLED: Autolysins, also known as peptidoglycan hydrolases, are enzymes that hydrolyze specific bonds within bacterial cell wall peptidoglycan during cell division and daughter cell separation. Within the genome of Lactobacillus acidophilus NCFM, there are 11 genes encoding proteins with peptidoglycan hydrolase catalytic domains, 9 of which are predicted to be functional. Notably, 5 of the 9 putative autolysins in L. acidophilus NCFM are S-layer-associated proteins (SLAPs) noncovalently colocalized along with the surface (S)-layer at the cell surface...
September 15, 2016: Applied and Environmental Microbiology
Jesús A Claudio-Rizo, Birzabith Mendoza-Novelo, Jorge Delgado, Laura E Castellano, José L Mata-Mata
This paper reports a new method to modify hydrogels derived from the acellular extracellular matrix (ECM) and consequently to improve their properties. The method is comprised of the combination of liquid precursors derived from hydrolyzed acellular small intestinal submucosa (hECM) and water-soluble oligourethanes that bear protected isocyanate groups, synthesized from poly(ethylene glycol) (PEG) and hexamethylene diisocyanate (HDI). The results demonstrate that the reactivity of oligourethanes, along with their water solubility, properly induce simultaneously the polymerization of type I collagen and its crosslinking...
2016: Biomedical Materials
Fanny Comblain, Jean-Emile Dubuc, Cécile Lambert, Christelle Sanchez, Isabelle Lesponne, Samuel Serisier, Yves Henrotin
OBJECTIVE: We have previously demonstrated that a mixture of curcuminoids extract, hydrolyzed collagen and green tea extract (COT) inhibited inflammatory and catabolic mediator's synthesis by osteoarthritic human chondrocytes. The objective of this study was to identify new targets of COT using genomic and proteomic approaches. DESIGN: Cartilage specimens were obtained from 12 patients with knee osteoarthritis. Primary human chondrocytes were cultured in monolayer until confluence and then incubated for 24 or 48 hours in the absence or in the presence of human interleukin(IL)-1β (10-11M) and with or without COT, each compound at the concentration of 4 μg/ml...
2016: PloS One
A R Sadeghi, S Nokhasteh, A M Molavi, M Khorsand-Ghayeni, H Naderi-Meshkin, A Mahdizadeh
In skin tissue engineering, surface feature of the scaffolds plays an important role in cell adhesion and proliferation. In this study, non-woven fibrous substrate based on poly (lactic-co-glycolic acid) (PLGA) (75/25) were hydrolyzed in various concentrations of NaOH (0.05N, 0.1N, 0.3N) to increase carboxyl and hydroxyl groups on the fiber surfaces. These functional groups were activated by EDC/NHS to create chemical bonding with collagen. To improve bioactivity, the activated substrates were coated with a collagen solution (2mg/ml) and cross-linking was carried out using the EDC/NHS in MES buffer...
September 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Renato M Ribeiro-Viana, Paula C S Faria-Tischer, Cesar A Tischer
The anhydroglucose chains of cellulose possess hydroxyls that facilitate different chemical modification strategies to expand on, or provide new applications for membranes produced by the bacteria Gluconacetobacter xylinus. Conjugation with biomolecules such as proteins, especially by the amine groups, is of great value and interest for the production of biomaterial derivatives from bacterial cellulose. To assist in these modifications, cellulose was succinylated in order to prevent steric hindrance and to create an attachment point for conjugation...
September 5, 2016: Carbohydrate Polymers
Jun-Hui Choi, Dae-Won Kim, Seung Kim, Sung-Jun Kim
A fibrinolytic metalloprotease with in vitro fibrinolytic effects was purified from the edible mushroom, Pleurotus ferulae using several chromatography steps including anion- and ion-exchange, gel filtration, and fast protein liquid chromatography (FPLC) columns. The molecular mass of the enzyme was estimated to be 20.0 kDa, as determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fibrin zymography. The protease was active at 50 °C, and pH 4.0, 5.0, and 8.0. The fibrinolytic activity of the enzyme was inhibited by EGTA, and strongly inhibited by two metal ions, Cu and Mg...
May 2, 2016: Preparative Biochemistry & Biotechnology
Agnes Virginia, Heni Rachmawati, Catur Riani, Debbie S Retnoningrum
Bioactive peptides produced from enzymatic hydrolysis fibrous protein have been proven to have several biological activities. Previous study showed that the hydrolysis product of snakehead fish skin collagen with 26 kDa collagenase from Bacillus licheniformis F11.4 showed HMG-CoA (HMGR) inhibition activity. The aim of this research was to determine the ability of the hydrolysis product produced from snakehead fish skin collagen hydrolysed by 50 kDa collagenase from B. licheniformis F11.4 in inhibiting HMGR activity...
2016: Scientia Pharmaceutica
Rodica Lipsa, Nita Tudorachi, Raluca Nicoleta Darie-Nita, Lacramioara Oprică, Cornelia Vasile, Aurica Chiriac
The purpose of this study was to assess the biodegradation of poly(lactic acid) (PLA) and some plasticized PLA based systems by Trichoderma viride fungus, in liquid medium and controlled laboratory conditions. The studied systems were achieved using PLA, hydrolyzed collagen (HC) as biological macromolecules and other additives by melt processing procedure. PLA and the systems' biodegradability was examined by the weight losses of the samples (after 7 and 21 days of exposure) and FTIR-ATR, GPC, SEM analyses (after 21 fungus inoculation days)...
July 2016: International Journal of Biological Macromolecules
José María Gutiérrez, Teresa Escalante, Alexandra Rucavado, Cristina Herrera
The historical development of discoveries and conceptual frames for understanding the hemorrhagic activity induced by viperid snake venoms and by hemorrhagic metalloproteinases (SVMPs) present in these venoms is reviewed. Histological and ultrastructural tools allowed the identification of the capillary network as the main site of action of SVMPs. After years of debate, biochemical developments demonstrated that all hemorrhagic toxins in viperid venoms are zinc-dependent metalloproteinases. Hemorrhagic SVMPs act by initially hydrolyzing key substrates at the basement membrane (BM) of capillaries...
April 2016: Toxins
J G Borges, A G Silva, C M Cervi-Bitencourt, F M Vanin, R A Carvalho
Orally disintegrating films (ODFs) can transport natural active compounds such as ethanol extract of propolis (EEP). This paper aimed to investigate the effect of lecithin on different gelatin and hydrolyzed collagen (HC) polymeric matrices with addition of EEP. ODFs were prepared by casting technique and were characterized (color parameters, water content, mechanical properties, microstructure, disintegration time (DT), infrared spectroscopy (FTIR), contact angle (CA), swelling degree and total phenolic content)...
May 2016: International Journal of Biological Macromolecules
Anna Takaoka, Natasha Babar, Julia Hogan, MiJung Kim, Marianne O Price, Francis W Price, Stephen L Trokel, David C Paik
PURPOSE: Current literature contains scant information regarding the extent of enzymatic collagen cross-linking in the keratoconus (KC) cornea. The aim of the present study was to examine levels of enzymatic lysyl oxidase-derived cross-links in stromal collagen in KC tissue, and to correlate the cross-link levels with collagen fibril stability as determined by thermal denaturation temperature (Tm). METHODS: Surgical KC samples (n = 17) and Eye-Bank control (n = 11) corneas of age 18 to 68 years were analyzed...
January 1, 2016: Investigative Ophthalmology & Visual Science
Hector L Lopez, Tim N Ziegenfuss, Joosang Park
CONTEXT: Little is known about the effect of nutritional supplementation on metabolic optimization for enhancing adaptation and recovery of the connective tissue elements that support musculoskeletal function. OBJECTIVE: The study aimed to determine the potential effect of supplementation with a novel, hydrolyzed chicken sternal cartilage extract-called BioCell Collagen-on biomarkers and functional indices of recovery from intense exercise. DESIGN: The research team designed a randomized, double-blind, placebo-controlled pilot study...
June 2015: Integrative Medicine
Yeon-Ji Jo, Jae-Hyeong Kim, Kyung-Hun Jung, Sang-Gi Min, Ji-Yeon Chun
Super- and sub-critical water treatments have been of interest as novel methods for protein hydrolysis. In the present study, we studied the effect of sub-critical water (Sub-H2O, 300℃, 80 bar) treatment as well as super-critical water (Super-H2O, 400℃, 280 bar) treatment on the physicochemical properties of porcine skin (PS), which has abundant collagen. Porcine skin was subjected to pre-thermal treatment by immersion in water at 70℃, and then treated with sub- or super-critical water. Physicochemical properties of the hydrolysates, such as molecular weight distribution, free amino acid content, amino acid profile, pH, color, and water content were determined...
2015: Korean Journal for Food Science of Animal Resources
Kyung-Hun Jung, Ye-Chul Choi, Ji-Yeon Chun, Sang-Gi Min, Geun-Pyo Hong
This study investigated the effects of three proteases (trypsin, pepsin and chymotrypsin) on the hydrolysis efficiency of porcine placenta and the molecular weight (Mw) distributions of the placental hydrolysates. Because placenta was made up of insoluble collagen, the placenta was gelatinized by applying thermal treatment at 90 ℃ for 1 h and used as the sample. The placental hydrolyzing activities of the enzymes at varying concentrations and incubation times were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel permeation chromatography (GPC)...
2014: Korean Journal for Food Science of Animal Resources
P A Mortarino, D P Goy, D B Abramson, J Cabello, G E Bumaguin, E J Vitelli, J Toledo, L Sarrio, S M Pezzotto, J P Mardegan Issa, G R Cointry, S Feldman
The induction of tolerance has been proposed as a therapeutic strategy for arthritis aiming to decrease progression of the pathology, probably by promoting suppressor mechanisms of the autoimmune response. This work aimed to confirm whether the treatment with vitamin D3 could synergize oral tolerance induced by hydrolyzed collagen peptides, in our experimental model of antigen induced arthritis in New Zealand rabbits. Clinical observation of the phenomenon indicates that simultaneous treatment with hydrolyzed collagen peptides and vitamin D3 was beneficial when compared with no treatment, for arthritic animals, and for arthritic animals that received treatment with only hydrolyzed collagen peptides or vitamin D3...
February 2016: Microscopy Research and Technique
Xiaohong Wang, Jian Huang, Kui Wang, Meik Neufurth, Heinz C Schröder, Shunfeng Wang, Werner E G Müller
Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the one caused by polyP (as calcium salt) and by GdCl3 alone, regarding their potencies to induce hydroxyapatite (HA) formation in SaOS-2 cells in vitro. The three compounds, GdCl3, polyP and polyP·Gd were found to be non-toxic at concentrations up to at least 30μM...
February 15, 2016: Biochemical Pharmacology
Chao Liu, Jiao Sun
Alveolar bone regeneration has aroused worldwide attention and plays an important role in oral clinics. In recent years, the application of biomaterials to induce osteogenic differentiation of periodontal ligament cells has become the hot topic in the field of alveolar bone regeneration. At present, most existing biomaterials lack osteoinductivity, while extrinsic inducers carry the risk of unwanted side effects. The objective of this work was to study the in vitro functionality of a newly developed hydrolyzed tilapia fish collagen (HFC) for periodontal tissue regeneration...
December 2015: Biomedical Materials
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