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Hydrolyzed Collagen

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https://www.readbyqxmd.com/read/27863377/variable-patterns-of-ectopic-mineralization-in-enpp1asj-2j-mice-a-model-for-generalized-arterial-calcification-of-infancy
#1
Sarah Y Siu, Nathaniel A Dyment, David W Rowe, John P Sundberg, Jouni Uitto, Qiaoli Li
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments...
November 14, 2016: Oncotarget
https://www.readbyqxmd.com/read/27862353/glutaraldehyde-collagen-cross-linking-stabilizes-resin-dentin-interfaces-and-reduces-bond-degradation
#2
Joshua Lee, Camila Sabatini
This study evaluated the stability of resin-dentin interfaces treated with glutaraldehyde-containing agents, and assessed collagen degradation in dentin matrices treated with Gluma. Microtensile bond strength (μTBS) was evaluated 24 h and 6 months after treatment with three desensitizers (Gluma Desensitizer, Gluma Desensitizer Power Gel, and MicroPrime G) and two etch-and-rinse adhesives (Comfort Bond & Desensitizer and iBond TE). Demineralized beams of human dentin were treated with water or Gluma, and the degradation of collagen in these beams was assessed by quantification of the dry mass loss and of the amount of hydroxyproline released from hydrolyzed specimens after 1 or 4 wk...
November 16, 2016: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/27815257/sirt3-blocks-myofibroblast-differentiation-and-pulmonary-fibrosis-by-preventing-mitochondrial-dna-damage
#3
Samik Bindu, Vinodkumar B Pillai, Abhinav Kanwal, Sadhana Samant, Gokhan Mutlu, Eric Verdin, Nickolai O Dulin, Mahesh P Gupta
Myofibroblast differentiation is a key process in the pathogenesis of fibrotic diseases. Transforming growth factor-β1 (TGFβ1) is a powerful inducer of myofibroblast differentiation and is implicated in pathogenensis of tissue fibrosis. This study was undertaken to determine the role of mitochondrial deacetylase, SIRT3 in TGF-β1-induced myofibroblast differentiqtion in vitro and lung fibrosis in vivo. Treatment of human lung fibroblasts with TGFβ1 resulted in increased expression of fibrosis markers, smooth muscle alpha-actin (α-SMA), collagen-1 and fibronectin...
November 4, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27789968/reduction-of-facial-wrinkles-by-hydrolyzed-water-soluble-egg-membrane-associated-with-reduction-of-free-radical-stress-and-support-of-matrix-production-by-dermal-fibroblasts
#4
Gitte S Jensen, Bijal Shah, Robert Holtz, Ashok Patel, Donald C Lo
OBJECTIVE: The aim of this study was to evaluate the effects of water-soluble egg membrane (WSEM) on wrinkle reduction in a clinical pilot study and to elucidate specific mechanisms of action using primary human immune and dermal cell-based bioassays. METHODS: To evaluate the effects of topical application of WSEM (8%) on human skin, an open-label 8-week study was performed involving 20 healthy females between the age of 45 years and 65 years. High-resolution photography and digital analysis were used to evaluate the wrinkle depth in the facial skin areas beside the eye (crow's feet)...
2016: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/27777467/characterization-of-swiftlet-edible-bird-nest-a-mucin-glycoprotein-and-its-adulterants-by-raman-microspectroscopy
#5
Eric K S Shim, Gleen F Chandra, S Pedireddy, Soo-Y Lee
Edible bird's nest (EBN) is made from the glutinous salivary secretion of highly concentrated mucin glycoprotein by swiftlets (genus Aerodramus or Collocalia) native to the Indo-Pacific region. The unique Raman spectrum of EBN has vibrational lines that can be assigned to peptides and saccharides in the glycoprotein, and it can be used to screen for adulteration. The common edible adulterants classified into two types. Type I adulterants, such as fish bladder, pork skin, karaya gum, coralline seaweed, agar strips, and tremella fungus, were solids which adhered externally on the surface of the EBN cement...
September 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27771442/serum-based-fluorescent-assay-for-evaluating-dipeptidyl-peptidase-i-activity-in-collagen-induced-arthritis-rat-model
#6
Xiaoqian Liu, Jingjing Wang, Yi Chu, Xiaoying Zhou
Dipeptidyl peptidase I (DPPI) is a lysosomal cysteine protease and derived from immune granule cells. It has been suggested playing an important role in the development of rheumatoid arthritis. In this study, a coumarin based fluorescent probe (GF-AFC) was designed and synthesized to evaluate DPPI activity in serum or tissue homogenates of collagen-induced arthritis (CIA) rats, an inflammatory arthropathy model. It was revealed that the fluorescent intensity was significantly increased in a very short time after specific substrate GF-AFC reacted with the DPPI...
October 19, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27722483/dual-component-collagenous-peptide-reactive-oligomer-hydrogels-as-potential-nerve-guidance-materials-from-characterization-to-functionalization
#7
C Kohn, J M Klemens, C Kascholke, N S Murthy, J Kohn, M Brandenburger, M C Hacker
Toward a new generation of improved nerve guidance conduits (NGCs), novel biomaterials are required to address pressing clinical shortcomings in peripheral nerve regeneration (PNR) and to promote biological performance. A dual-component hydrogel system formed by cross-linking reaction between maleic anhydride groups in an oligomeric building block for cross-linking of free amine functionalities in partially hydrolyzed collagen is formulated for continuous processing and NGC fabrication. The influence of the gelation base is optimized for processing from a double syringe delivery system with a static mixer...
October 18, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27662266/the-role-of-three-dimensional-pure-bovine-gelatin-scaffolds-in-tendon-healing-modeling-and-remodeling-an-in-vivo-investigation-with-potential-clinical-value
#8
Ahmad Oryan, Pardis Sharifi, Ali Moshiri, Ian A Silver
AIM OF THE STUDY: Large tendon defects involving extensive tissue loss present complex clinical problems. Surgical reconstruction of such injuries is normally performed by transplanting autogenous and allogenous soft tissues that are expected to remodel to mimic a normal tendon. However, the use of grafts has always been associated with significant limitations. Tissue engineering employing artificial scaffolds may provide acceptable alternatives. Gelatin is a hydrolyzed form of collagen that is bioactive, biodegradable, and biocompatible...
September 23, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27552601/biochemical-properties-and-evaluation-of-washing-performance-in-commercial-detergent-compatibility-of-two-collagenolytic-serine-peptidases-secreted-by-aspergillus-fischeri-and-penicillium-citrinum
#9
Érika Lika Ida, Ronivaldo Rodrigues da Silva, Tássio Brito de Oliveira, Tatiane Beltramini Souto, Juliana Abigail Leite, André Rodrigues, Hamilton Cabral
Filamentous fungi secrete diverse peptidases with different biochemical properties, which is of considerable importance for application in various commercial sectors. In this study, we describe the isolation of two fungal species collected from the soil of decayed organic matter: Aspergillus fischeri and Penicillium citrinum. In a submerged bioprocess, we observed better peptidase production with the fungus P. citrinum, which reached a peak production at 168 h with 760 U/mL, in comparison to that with the fungus A...
August 23, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27422832/acmb-is-an-s-layer-associated-%C3%AE-n-acetylglucosaminidase-and-functional-autolysin-in-lactobacillus-acidophilus-ncfm
#10
Brant R Johnson, Todd R Klaenhammer
UNLABELLED: Autolysins, also known as peptidoglycan hydrolases, are enzymes that hydrolyze specific bonds within bacterial cell wall peptidoglycan during cell division and daughter cell separation. Within the genome of Lactobacillus acidophilus NCFM, there are 11 genes encoding proteins with peptidoglycan hydrolase catalytic domains, 9 of which are predicted to be functional. Notably, 5 of the 9 putative autolysins in L. acidophilus NCFM are S-layer-associated proteins (SLAPs) noncovalently colocalized along with the surface (S)-layer at the cell surface...
September 15, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27305317/a-new-method-for-the-preparation-of-biomedical-hydrogels-comprised-of-extracellular-matrix-and-oligourethanes
#11
Jesús A Claudio-Rizo, Birzabith Mendoza-Novelo, Jorge Delgado, Laura E Castellano, José L Mata-Mata
This paper reports a new method to modify hydrogels derived from the acellular extracellular matrix (ECM) and consequently to improve their properties. The method is comprised of the combination of liquid precursors derived from hydrolyzed acellular small intestinal submucosa (hECM) and water-soluble oligourethanes that bear protected isocyanate groups, synthesized from poly(ethylene glycol) (PEG) and hexamethylene diisocyanate (HDI). The results demonstrate that the reactivity of oligourethanes, along with their water solubility, properly induce simultaneously the polymerization of type I collagen and its crosslinking...
2016: Biomedical Materials
https://www.readbyqxmd.com/read/27275599/identification-of-targets-of-a-new-nutritional-mixture-for-osteoarthritis-management-composed-by-curcuminoids-extract-hydrolyzed-collagen-and-green-tea-extract
#12
Fanny Comblain, Jean-Emile Dubuc, Cécile Lambert, Christelle Sanchez, Isabelle Lesponne, Samuel Serisier, Yves Henrotin
OBJECTIVE: We have previously demonstrated that a mixture of curcuminoids extract, hydrolyzed collagen and green tea extract (COT) inhibited inflammatory and catabolic mediator's synthesis by osteoarthritic human chondrocytes. The objective of this study was to identify new targets of COT using genomic and proteomic approaches. DESIGN: Cartilage specimens were obtained from 12 patients with knee osteoarthritis. Primary human chondrocytes were cultured in monolayer until confluence and then incubated for 24 or 48 hours in the absence or in the presence of human interleukin(IL)-1β (10-11M) and with or without COT, each compound at the concentration of 4 μg/ml...
2016: PloS One
https://www.readbyqxmd.com/read/27207046/surface-modification-of-electrospun-plga-scaffold-with-collagen-for-bioengineered-skin-substitutes
#13
A R Sadeghi, S Nokhasteh, A M Molavi, M Khorsand-Ghayeni, H Naderi-Meshkin, A Mahdizadeh
In skin tissue engineering, surface feature of the scaffolds plays an important role in cell adhesion and proliferation. In this study, non-woven fibrous substrate based on poly (lactic-co-glycolic acid) (PLGA) (75/25) were hydrolyzed in various concentrations of NaOH (0.05N, 0.1N, 0.3N) to increase carboxyl and hydroxyl groups on the fiber surfaces. These functional groups were activated by EDC/NHS to create chemical bonding with collagen. To improve bioactivity, the activated substrates were coated with a collagen solution (2mg/ml) and cross-linking was carried out using the EDC/NHS in MES buffer...
September 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27185111/preparation-of-succinylated-cellulose-membranes-for-functionalization-purposes
#14
Renato M Ribeiro-Viana, Paula C S Faria-Tischer, Cesar A Tischer
The anhydroglucose chains of cellulose possess hydroxyls that facilitate different chemical modification strategies to expand on, or provide new applications for membranes produced by the bacteria Gluconacetobacter xylinus. Conjugation with biomolecules such as proteins, especially by the amine groups, is of great value and interest for the production of biomaterial derivatives from bacterial cellulose. To assist in these modifications, cellulose was succinylated in order to prevent steric hindrance and to create an attachment point for conjugation...
September 5, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27136080/purification-and-partial-characterization-of-a-fibrinolytic-enzyme-from-the-fruiting-body-of-the-medicinal-and-edible-mushroom-pleurotus-ferulae
#15
Jun-Hui Choi, Dae-Won Kim, Seung Kim, Sung-Jun Kim
A fibrinolytic metalloprotease with in vitro fibrinolytic effects was purified from the edible mushroom, Pleurotus ferulae using several chromatography steps including anion- and ion-exchange, gel filtration, and fast protein liquid chromatography (FPLC) columns. The molecular mass of the enzyme was estimated to be 20.0 kDa, as determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fibrin zymography. The protease was active at 50 °C, and pH 4.0, 5.0, and 8.0. The fibrinolytic activity of the enzyme was inhibited by EGTA, and strongly inhibited by two metal ions, Cu and Mg...
May 2, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27110500/study-of-hmg-coa-reductase-inhibition-activity-of-the-hydrolyzed-product-of-snakehead-fish-channa-striata-skin-collagen-with-50-kda-collagenase-from-bacillus-licheniformis-f11-4
#16
Agnes Virginia, Heni Rachmawati, Catur Riani, Debbie S Retnoningrum
Bioactive peptides produced from enzymatic hydrolysis fibrous protein have been proven to have several biological activities. Previous study showed that the hydrolysis product of snakehead fish skin collagen with 26 kDa collagenase from Bacillus licheniformis F11.4 showed HMG-CoA (HMGR) inhibition activity. The aim of this research was to determine the ability of the hydrolysis product produced from snakehead fish skin collagen hydrolysed by 50 kDa collagenase from B. licheniformis F11.4 in inhibiting HMGR activity...
January 2016: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/27064086/biodegradation-of-poly-lactic-acid-and-some-of-its-based-systems-with-trichoderma-viride
#17
Rodica Lipsa, Nita Tudorachi, Raluca Nicoleta Darie-Nita, Lacramioara Oprică, Cornelia Vasile, Aurica Chiriac
The purpose of this study was to assess the biodegradation of poly(lactic acid) (PLA) and some plasticized PLA based systems by Trichoderma viride fungus, in liquid medium and controlled laboratory conditions. The studied systems were achieved using PLA, hydrolyzed collagen (HC) as biological macromolecules and other additives by melt processing procedure. PLA and the systems' biodegradability was examined by the weight losses of the samples (after 7 and 21 days of exposure) and FTIR-ATR, GPC, SEM analyses (after 21 fungus inoculation days)...
July 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27023608/hemorrhage-caused-by-snake-venom-metalloproteinases-a-journey-of-discovery-and-understanding
#18
REVIEW
José María Gutiérrez, Teresa Escalante, Alexandra Rucavado, Cristina Herrera
The historical development of discoveries and conceptual frames for understanding the hemorrhagic activity induced by viperid snake venoms and by hemorrhagic metalloproteinases (SVMPs) present in these venoms is reviewed. Histological and ultrastructural tools allowed the identification of the capillary network as the main site of action of SVMPs. After years of debate, biochemical developments demonstrated that all hemorrhagic toxins in viperid venoms are zinc-dependent metalloproteinases. Hemorrhagic SVMPs act by initially hydrolyzing key substrates at the basement membrane (BM) of capillaries...
April 2016: Toxins
https://www.readbyqxmd.com/read/26826291/lecithin-gelatin-and-hydrolyzed-collagen-orally-disintegrating-films-functional-properties
#19
J G Borges, A G Silva, C M Cervi-Bitencourt, F M Vanin, R A Carvalho
Orally disintegrating films (ODFs) can transport natural active compounds such as ethanol extract of propolis (EEP). This paper aimed to investigate the effect of lecithin on different gelatin and hydrolyzed collagen (HC) polymeric matrices with addition of EEP. ODFs were prepared by casting technique and were characterized (color parameters, water content, mechanical properties, microstructure, disintegration time (DT), infrared spectroscopy (FTIR), contact angle (CA), swelling degree and total phenolic content)...
May 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26780316/an-evaluation-of-lysyl-oxidase-derived-cross-linking-in-keratoconus-by-liquid-chromatography-mass-spectrometry
#20
Anna Takaoka, Natasha Babar, Julia Hogan, MiJung Kim, Marianne O Price, Francis W Price, Stephen L Trokel, David C Paik
PURPOSE: Current literature contains scant information regarding the extent of enzymatic collagen cross-linking in the keratoconus (KC) cornea. The aim of the present study was to examine levels of enzymatic lysyl oxidase-derived cross-links in stromal collagen in KC tissue, and to correlate the cross-link levels with collagen fibril stability as determined by thermal denaturation temperature (Tm). METHODS: Surgical KC samples (n = 17) and Eye-Bank control (n = 11) corneas of age 18 to 68 years were analyzed...
January 1, 2016: Investigative Ophthalmology & Visual Science
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