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Hydrolyzed Collagen

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https://www.readbyqxmd.com/read/28715873/subcritical-water-hydrolyzed-fish-collagen-ameliorates-survival-of-endotoxemic-mice-by-inhibiting-hmgb1-release-in-a-ho-1-dependent-manner
#1
Min Young Ahn, Jung Seok Hwang, Sun Ah Ham, Jinwoo Hur, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Sung Gu Han, Han Geuk Seo
To investigate potential mechanisms underlying the bioactivity of hydrolyzed fish collagen, we examined the anti-inflammatory actions of subcritical water-hydrolyzed fish collagen (SWFC) in lipopolysaccharide (LPS)-triggered inflammation and endotoxemia. SWFC markedly inhibited LPS-stimulated release of high mobility group box 1 (HMGB1) in murine RAW264.7 macrophages, along with decreased cytosolic translocation of HMGB1. Both the protein and mRNA levels of heme oxygenase-1 (HO-1) were significantly upregulated in SWFC-treated RAW 264...
July 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28707490/transferon%C3%A2-a-peptide-mixture-with-immunomodulatory-properties-is-not-immunogenic-when-administered-with-various-adjuvants
#2
Sandra Avila, Leslie Muñoz-García, Said Vázquez-Leyva, Nohemí Salinas-Jazmín, Emilio Medina-Rivero, Lenin Pavón, Gabriela Mellado-Sánchez, Rommel Chacón-Salinas, Sergio Estrada-Parra, Luis Vallejo-Castillo, Sonia Mayra Pérez-Tapia
Transferon, a human dialyzable leukocyte extract (hDLE), is a biotherapeutic that comprises a complex mixture of low-molecular-weight peptides (< 10 kDa) and is used to treat diseases with an inflammatory component. Some biotherapeutics, including those composed of peptides, can induce anti-drug antibodies (ADA) that block or diminish their therapeutic effect. Nevertheless, few studies have evaluated peptide-derived drug immunogenicity. In this study, the immunogenicity of Transferon was examined in a murine model during an immunization scheme using the following adjuvants: Al(OH)3, incomplete Freund's adjuvant (IFA), or Titermax Gold...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28678821/a-casein-hydrolysate-based-formulation-attenuates-obesity-and-associated-non-alcoholic-fatty-liver-disease-and-atherosclerosis-in-ldlr-leiden-mice
#3
Marieke H Schoemaker, Robert Kleemann, Martine C Morrison, Joanne Verheij, Kanita Salic, Eric A F van Tol, Teake Kooistra, Peter Y Wielinga
BACKGROUND: Obesity frequently associates with the development of non-alcoholic fatty liver disease (NAFLD) and atherosclerosis. Chronic inflammation in white adipose tissue (WAT) seems to be an important driver of these manifestations. OBJECTIVE: This study investigated a combination of an extensively hydrolyzed casein (eHC), docosahexaenoic acid (DHA), arachidonic acid (ARA), and Lactobacillus Rhamnosus GG (LGG) (together referred to as nutritional ingredients, NI) on the development of obesity, metabolic risk factors, WAT inflammation, NAFLD and atherosclerosis in high-fat diet-fed LDLr-/-...
2017: PloS One
https://www.readbyqxmd.com/read/28677335/substrate-specificity-and-reaction-mechanism-of-human-prolidase
#4
Piotr Wilk, Monika Uehlein, Jacquelin Kalms, Holger Dobbek, Uwe Mueller, Manfred S Weiss
Prolidase is a ubiquitously distributed dipeptidase and the only dipeptidase in humans capable of cleaving the peptide bond preceding the amino acids proline (Pro) or hydroxyproline (Hyp). It is mainly implicated in the degradation of dietary and endogenous proteins. It is also involved in the terminal steps of collagen catabolism by hydrolyzing Pro and Hyp-containing dipeptides. Finally, it is believed to play a role in the regulation of peptidic hormones. Diminished or absent prolidase activity is related to a rare autosomal disease, referred to as prolidase deficiency (PD)...
July 4, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28602994/fish-collagen-peptide-inhibits-the-adipogenic-differentiation-of-preadipocytes-and-ameliorates-obesity-in-high-fat-diet-fed-mice
#5
Eun Ji Lee, Jinwoo Hur, Sun Ah Ham, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Han Geuk Seo
Bioactivities of fish collagen peptide are now being elucidated in diverse biological systems. Here, we investigated the effect of fish collagen peptide on the adipogenic differentiation of 3T3-L1 preadipocytes and in obese mice fed a high fat diet (HFD). Subcritical water-hydrolyzed fish collagen peptide (SWFCP) significantly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes, which was accompanied by decreased expression of CCAAT-enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2) genes, key regulators of differentiation and maintenance of adipocytes...
June 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28576050/extraction-and-characterization-of-collagen-from-antarctic-and-sub-antarctic-squid-and-its-potential-application-in-hybrid-scaffolds-for-tissue-engineering
#6
Rui C G Coelho, Ana L P Marques, Sara M Oliveira, Gabriela S Diogo, Rogério P Pirraco, Joana Moreira-Silva, José C Xavier, Rui L Reis, Tiago H Silva, João F Mano
Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28569344/radiofrequency-treatment-induces-fibroblast-growth-factor-2-expression-and-subsequently-promotes-neocollagenesis-and-neoangiogenesis-in-the-skin-tissue
#7
Patrícia Froes Meyer, Patrícia de Oliveira, Flávia K B A Silva, Ana C S da Costa, Caline R A Pereira, Sebastian Casenave, Rodrigo Marcel Valentim Silva, Luis Gonzaga Araújo-Neto, Sebastião David Santos-Filho, Eric Aizamaque, Hennes Gentil Araújo, Mario Bernardo-Filho, Maria Goretti Freire Carvalho, Ciro Dantas Soares
Radiofrequency (RF) treatment appears to be involved in production of new collagen fibrils and the improvement of existing collagen structures; however, the molecular bases of the effect of non-invasive RF on the skin tissue have not been fully elucidated. This study reports the effects of RF associated or not with hydrolyzed collagen (HC) in the skin tissue. Wistar rats were randomly divided into four groups, according to the treatment received: control group (G1, n = 5), no treatment; subjects in group G2 (n = 5) were treated with HC; and capacitive RF was applied to the back of each subject in G3 (n = 5) and RF associated with HC in G4 (n = 5)...
May 31, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28499097/substantivity-of-carbodiimide-inhibition-on-dentinal-enzyme-activity-over-time
#8
A Mazzoni, V Angeloni, N Sartori, S Duarte, T Maravic, L Tjäderhane, D H Pashley, F R Tay, L Breschi
The use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) has recently been investigated for its effectiveness in the prevention of collagen degradation over time and the improvement of resin-dentin bond durability. The objective of the present study was to evaluate the effects of a 0.3 M EDC-containing conditioner on endogenous enzymatic activities within the hybrid layer (HL) created by a self-etch or an etch-and-rinse adhesive after 1 y. The activity within the HL was examined using in situ zymography and confocal laser scanning microscopy after 24 h or 1-y storage in artificial saliva...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28475143/hydrolysates-of-fish-skin-collagen-an-opportunity-for-valorizing-fish-industry-byproducts
#9
María Blanco, José Antonio Vázquez, Ricardo I Pérez-Martín, Carmen G Sotelo
During fish processing operations, such as skinning and filleting, the removal of collagen-containing materials can account for up to 30% of the total fish byproducts. Collagen is the main structural protein in skin, representing up to 70% of dry weight depending on the species, age and season. It has a wide range of applications including cosmetic, pharmaceutical, food industry, and medical. In the present work, collagen was obtained by pepsin extraction from the skin of two species of teleost and two species of chondrychtyes with yields varying between 14...
May 5, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28460927/angiotensin-i-converting-enzyme-inhibitory-peptides-from-bovine-collagen-insights-into-inhibitory-mechanism-and-transepithelial-transport
#10
Yu Fu, Jette Feveile Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E Aluko, Margrethe Therkildsen
The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed bovine collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF)...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28455445/the-soluble-protease-adamdec1-released-from-activated-platelets-hydrolyzes-platelet-membrane-pro-epidermal-growth-factor-egf-to-active-high-molecular-weight-egf
#11
Rui Chen, Ge Jin, Thomas M McIntyre
Platelets are the sole source of EGF in circulation, yet how EGF is stored or released from stimulated cells is undefined. In fact, we found platelets did not store EGF, synthesized as a single 6-kDa domain in pro-EGF, but rather expressed intact pro-EGF precursor on granular and plasma membranes. Activated platelets released high-molecular-weight (HMW)-EGF, produced by a single cleavage between the EGF and the transmembrane domains of pro-EGF. We synthesized a fluorogenic peptide encompassing residues surrounding the putative sessile arginyl residue and found stimulated platelets released soluble activity that cleaved this pro-EGF1020-1027 peptide...
June 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28384173/daily-oral-consumption-of-hydrolyzed-type-1-collagen-is-chondroprotective-and-anti-inflammatory-in-murine-posttraumatic-osteoarthritis
#12
Qurratul-Ain Dar, Eric M Schott, Sarah E Catheline, Robert D Maynard, Zhaoyang Liu, Fadia Kamal, Christopher W Farnsworth, John P Ketz, Robert A Mooney, Matthew J Hilton, Jennifer H Jonason, Janne Prawitt, Michael J Zuscik
Osteoarthritis (OA) is a degenerative joint disease for which there are no disease modifying therapies. Thus, strategies that offer chondroprotective or regenerative capability represent a critical unmet need. Recently, oral consumption of a hydrolyzed type 1 collagen (hCol1) preparation has been reported to reduce pain in human OA and support a positive influence on chondrocyte function. To evaluate the tissue and cellular basis for these effects, we examined the impact of orally administered hCol1 in a model of posttraumatic OA (PTOA)...
2017: PloS One
https://www.readbyqxmd.com/read/28244315/oral-ingestion-of-collagen-hydrolysate-leads-to-the-transportation-of-highly-concentrated-gly-pro-hyp-and-its-hydrolyzed-form-of-pro-hyp-into-the-bloodstream-and-skin
#13
Misato Yazaki, Yukihiko Ito, Masayoshi Yamada, Spyros Goulas, Sachiyuki Teramoto, Masa-Aki Nakaya, Shigeo Ohno, Kohji Yamaguchi
Collagen hydrolysate is a well-known dietary supplement for the treatment of skin aging; however, its mode of action remains unknown. Previous studies have shown that the oral ingestion of collagen hydrolysate leads to elevated levels of collagen-derived peptides in the blood, but whether these peptides reach the skin remains unclear. Here, we analyzed the plasma concentration of collagen-derived peptides after ingestion of high tripeptide containing collagen hydrolysate in humans. We identified 17 types of collagen-derived peptides transiently, with a particular enrichment in Gly-Pro-Hyp...
March 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28235624/design-of-novel-injectable-in-situ-forming-scaffolds-for-non-surgical-treatment-of-periapical-lesions-in-vitro-and-in-vivo-evaluation
#14
Rehab N Shamma, Nermeen A Elkasabgy, Azza A Mahmoud, Shaimaa I Gawdat, Mohamed M Kataia, Mohamed A Abdel Hamid
Periapical lesions are considered one of the common pathological conditions affecting alveolar bone. The primary focus of this study was to investigate the effectiveness of formulating an injectable in-situ forming scaffold-loaded with risedronate (bone resorption inhibitor) and with lornoxicam (anti-inflammatory drug) for the non-surgical treatment of periapical lesions. The scaffolds were prepared using solvent-induced phase inversion technique. Two insoluble copolymers were investigated namely; PLGA (ester-terminal) and PLGA-A (acid-terminal), additionally, SAIB was added as a high viscosity water-insoluble carrier...
April 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28176961/blooming-gelatin-an-individual-additive-for-enhancing-nanoapatite-precipitation-physical-properties-and-osteoblastic-responses-of-nanostructured-macroporous-calcium-phosphate-bone-cements
#15
Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani
In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28159262/transepithelial-transport-efficiency-of-bovine-collagen-hydrolysates-in-a-human-caco-2-cell-line-model
#16
Mengmeng Feng, Mirko Betti
Collagen was extracted from raw bovine hide and hydrolyzed by one of three enzymes - Alcalase, Flavourzyme, or trypsin - or by using a combination of two or three of these enzymes. The Alcalase-containing enzymatic hydrolysis treatments generated a greater proportion of hydrolysates with molecular weight (MW) <2kDa (79.8-82.7%). Flavourzyme-containing hydrolysis treatments exhibited the greatest proportion of free amino acids (686-740nmol/mg). The hydrolysates were then subjected to a simulated gastrointestinal (GI) digestion, and transport studies were conducted using a Caco-2 cell model...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28038947/ph-sensitive-polymeric-micelles-for-targeted-delivery-to-inflamed-joints
#17
Chunhong Li, Hanmei Li, Qin Wang, Meiling Zhou, Man Li, Tao Gong, Zhirong Zhang, Xun Sun
Effective treatment for rheumatoid arthritis is hindered by the lack of drugs that selectively target inflamed joints. Liposomes, nanoparticles and conventional micelles loaded with limited amounts of drugs may be unstable in the circulation and result in uncontrolled drug release kinetics. Here we developed a new drug delivery system of pH-sensitive polymeric micelles based on an acid-labile hydrazone bond. Amphiphilic conjugates of a PEG-based derivative and the hydrophobic drug prednisolone (PD) self-assembled into PD micelles with a drug loading of 19...
January 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27999369/protective-effects-of-hydrolyzed-nucleoproteins-from-salmon-milt-against-ethanol-induced-liver-injury-in-rats
#18
Akiko Kojima-Yuasa, Mayu Goto, Eri Yoshikawa, Yuri Morita, Hirotaka Sekiguchi, Keita Sutoh, Koji Usumi, Isao Matsui-Yuasa
Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In this study, we examined the effects of DNA-rich nucleic acids prepared from salmon milt (DNSM) on the development of liver fibrosis in an in vivo ethanol-carbon tetrachloride cirrhosis model. Plasma aspartate transaminase and alanine transaminase were significantly less active in the DNSM-treated group than in the ethanol plus carbon tetrachloride (CCl₄)-treated group...
December 19, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27932058/tnap-stimulates-vascular-smooth-muscle-cell-trans-differentiation-into-chondrocytes-through-calcium-deposition-and-bmp-2-activation-possible-implication-in-atherosclerotic-plaque-stability
#19
Maya Fakhry, Monika Roszkowska, Anne Briolay, Carole Bougault, Alain Guignandon, Juan Ignacio Diaz-Hernandez, Miguel Diaz-Hernandez, Slawomir Pikula, René Buchet, Eva Hamade, Bassam Badran, Laurence Bessueille, David Magne
Atherosclerotic plaque calcification varies from early, diffuse microcalcifications to a bone-like tissue formed by endochondral ossification. Recently, a paradigm has emerged suggesting that if the bone metaplasia stabilizes the plaques, microcalcifications are harmful. Tissue-nonspecific alkaline phosphatase (TNAP), an ectoenzyme necessary for mineralization by its ability to hydrolyze inorganic pyrophosphate (PPi), is stimulated by inflammation in vascular smooth muscle cells (VSMCs). Our objective was to determine the role of TNAP in trans-differentiation of VSMCs and calcification...
December 6, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27863377/variable-patterns-of-ectopic-mineralization-in-enpp1asj-2j-mice-a-model-for-generalized-arterial-calcification-of-infancy
#20
Sarah Y Siu, Nathaniel A Dyment, David W Rowe, John P Sundberg, Jouni Uitto, Qiaoli Li
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments...
December 20, 2016: Oncotarget
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