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Hydrolyzed Collagen

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https://www.readbyqxmd.com/read/28499097/substantivity-of-carbodiimide-inhibition-on-dentinal-enzyme-activity-over-time
#1
A Mazzoni, V Angeloni, N Sartori, S Duarte, T Maravic, L Tjäderhane, D H Pashley, F R Tay, L Breschi
The use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) has recently been investigated for its effectiveness in the prevention of collagen degradation over time and the improvement of resin-dentin bond durability. The objective of the present study was to evaluate the effects of a 0.3 M EDC-containing conditioner on endogenous enzymatic activities within the hybrid layer (HL) created by a self-etch or an etch-and-rinse adhesive after 1 y. The activity within the HL was examined using in situ zymography and confocal laser scanning microscopy after 24 h or 1-y storage in artificial saliva...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28475143/hydrolysates-of-fish-skin-collagen-an-opportunity-for-valorizing-fish-industry-byproducts
#2
María Blanco, José Antonio Vázquez, Ricardo I Pérez-Martín, Carmen G Sotelo
During fish processing operations, such as skinning and filleting, the removal of collagen-containing materials can account for up to 30% of the total fish byproducts. Collagen is the main structural protein in skin, representing up to 70% of dry weight depending on the species, age and season. It has a wide range of applications including cosmetic, pharmaceutical, food industry, and medical. In the present work, collagen was obtained by pepsin extraction from the skin of two species of teleost and two species of chondrychtyes with yields varying between 14...
May 5, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28460927/angiotensin-i-converting-enzyme-inhibitory-peptides-from-bovine-collagen-insights-into-inhibitory-mechanism-and-transepithelial-transport
#3
Yu Fu, Jette Feveile Young, Martin Krøyer Rasmussen, Trine Kastrup Dalsgaard, René Lametsch, Rotimi E Aluko, Margrethe Therkildsen
The inhibitory mechanism and transepithelial transport of angiotensin I-converting enzyme (ACE)-inhibitory peptides (VGPV and GPRGF) derived from Alcalase®- and papain-hydrolyzed bovine collagen were investigated. The inhibitory mechanism of VGPV and GPRGF was experimentally determined to be non-competitive and the results were supported by molecular docking data. In silico and in vitro gastrointestinal digestion indicated that VGPV remained resistant to digestive enzymes, while GPRGF was degraded into smaller ACE-inhibitory peptides (GPR and GF)...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28455445/the-soluble-protease-adamdec1-released-from-activated-platelets-hydrolyzes-platelet-membrane-pro-egf-to-active-high-molecular-weight-egf
#4
Rui Chen, Ge Jin, Thomas M McIntyre
Platelets are the sole source of EGF in the circulation, yet how EGF is stored or released from stimulated cells are undefined. In fact, we found platelets did not store EGF, synthesized as a single 6 kDa domain in pro-EGF, but rather expressed intact pro-EGF precursor on granular and plasma membranes. Activated platelets released high-molecular-weight (HMW)-EGF, produced by a single cleavage between the EGF and transmembrane domains of pro-EGF. We synthesized a fluorogenic peptide encompassing residues surrounding the putative sessile arginyl residue and found stimulated platelets released a soluble activity that cleaved this pro-EGF1020-1027 peptide...
April 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28384173/daily-oral-consumption-of-hydrolyzed-type-1-collagen-is-chondroprotective-and-anti-inflammatory-in-murine-posttraumatic-osteoarthritis
#5
Qurratul-Ain Dar, Eric M Schott, Sarah E Catheline, Robert D Maynard, Zhaoyang Liu, Fadia Kamal, Christopher W Farnsworth, John P Ketz, Robert A Mooney, Matthew J Hilton, Jennifer H Jonason, Janne Prawitt, Michael J Zuscik
Osteoarthritis (OA) is a degenerative joint disease for which there are no disease modifying therapies. Thus, strategies that offer chondroprotective or regenerative capability represent a critical unmet need. Recently, oral consumption of a hydrolyzed type 1 collagen (hCol1) preparation has been reported to reduce pain in human OA and support a positive influence on chondrocyte function. To evaluate the tissue and cellular basis for these effects, we examined the impact of orally administered hCol1 in a model of posttraumatic OA (PTOA)...
2017: PloS One
https://www.readbyqxmd.com/read/28244315/oral-ingestion-of-collagen-hydrolysate-leads-to-the-transportation-of-highly-concentrated-gly-pro-hyp-and-its-hydrolyzed-form-of-pro-hyp-into-the-bloodstream-and-skin
#6
Misato Yazaki, Yukihiko Ito, Masayoshi Yamada, Spyros Goulas, Sachiyuki Teramoto, Masa-Aki Nakaya, Shigeo Ohno, Kohji Yamaguchi
Collagen hydrolysate is a well-known dietary supplement for the treatment of skin aging; however, its mode of action remains unknown. Previous studies have shown that the oral ingestion of collagen hydrolysate leads to elevated levels of collagen-derived peptides in the blood, but whether these peptides reach the skin remains unclear. Here, we analyzed the plasma concentration of collagen-derived peptides after ingestion of high tripeptide containing collagen hydrolysate in humans. We identified 17 types of collagen-derived peptides transiently, with a particular enrichment in Gly-Pro-Hyp...
March 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28235624/design-of-novel-injectable-in-situ-forming-scaffolds-for-non-surgical-treatment-of-periapical-lesions-in-vitro-and-in-vivo-evaluation
#7
Rehab N Shamma, Nermeen A Elkasabgy, Azza A Mahmoud, Shaimaa I Gawdat, Mohamed M Kataia, Mohamed A Abdel Hamid
Periapical lesions are considered one of the common pathological conditions affecting alveolar bone. The primary focus of this study was to investigate the effectiveness of formulating an injectable in-situ forming scaffold-loaded with risedronate (bone resorption inhibitor) and with lornoxicam (anti-inflammatory drug) for the non-surgical treatment of periapical lesions. The scaffolds were prepared using solvent-induced phase inversion technique. Two insoluble copolymers were investigated namely; PLGA (ester-terminal) and PLGA-A (acid-terminal), additionally, SAIB was added as a high viscosity water-insoluble carrier...
April 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28176961/blooming-gelatin-an-individual-additive-for-enhancing-nanoapatite-precipitation-physical-properties-and-osteoblastic-responses-of-nanostructured-macroporous-calcium-phosphate-bone-cements
#8
Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani
In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28159262/transepithelial-transport-efficiency-of-bovine-collagen-hydrolysates-in-a-human-caco-2-cell-line-model
#9
Mengmeng Feng, Mirko Betti
Collagen was extracted from raw bovine hide and hydrolyzed by one of three enzymes - Alcalase, Flavourzyme, or trypsin - or by using a combination of two or three of these enzymes. The Alcalase-containing enzymatic hydrolysis treatments generated a greater proportion of hydrolysates with molecular weight (MW) <2kDa (79.8-82.7%). Flavourzyme-containing hydrolysis treatments exhibited the greatest proportion of free amino acids (686-740nmol/mg). The hydrolysates were then subjected to a simulated gastrointestinal (GI) digestion, and transport studies were conducted using a Caco-2 cell model...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28038947/ph-sensitive-polymeric-micelles-for-targeted-delivery-to-inflamed-joints
#10
Chunhong Li, Hanmei Li, Qin Wang, Meiling Zhou, Man Li, Tao Gong, Zhirong Zhang, Xun Sun
Effective treatment for rheumatoid arthritis is hindered by the lack of drugs that selectively target inflamed joints. Liposomes, nanoparticles and conventional micelles loaded with limited amounts of drugs may be unstable in the circulation and result in uncontrolled drug release kinetics. Here we developed a new drug delivery system of pH-sensitive polymeric micelles based on an acid-labile hydrazone bond. Amphiphilic conjugates of a PEG-based derivative and the hydrophobic drug prednisolone (PD) self-assembled into PD micelles with a drug loading of 19...
January 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27999369/protective-effects-of-hydrolyzed-nucleoproteins-from-salmon-milt-against-ethanol-induced-liver-injury-in-rats
#11
Akiko Kojima-Yuasa, Mayu Goto, Eri Yoshikawa, Yuri Morita, Hirotaka Sekiguchi, Keita Sutoh, Koji Usumi, Isao Matsui-Yuasa
Dietary nucleotides play a role in maintaining the immune responses of both animals and humans. Oral administration of nucleic acids from salmon milt have physiological functions in the cellular metabolism, proliferation, differentiation, and apoptosis of human small intestinal epithelial cells. In this study, we examined the effects of DNA-rich nucleic acids prepared from salmon milt (DNSM) on the development of liver fibrosis in an in vivo ethanol-carbon tetrachloride cirrhosis model. Plasma aspartate transaminase and alanine transaminase were significantly less active in the DNSM-treated group than in the ethanol plus carbon tetrachloride (CCl₄)-treated group...
December 19, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27932058/tnap-stimulates-vascular-smooth-muscle-cell-trans-differentiation-into-chondrocytes-through-calcium-deposition-and-bmp-2-activation-possible-implication-in-atherosclerotic-plaque-stability
#12
Maya Fakhry, Monika Roszkowska, Anne Briolay, Carole Bougault, Alain Guignandon, Juan Ignacio Diaz-Hernandez, Miguel Diaz-Hernandez, Slawomir Pikula, René Buchet, Eva Hamade, Bassam Badran, Laurence Bessueille, David Magne
Atherosclerotic plaque calcification varies from early, diffuse microcalcifications to a bone-like tissue formed by endochondral ossification. Recently, a paradigm has emerged suggesting that if the bone metaplasia stabilizes the plaques, microcalcifications are harmful. Tissue-nonspecific alkaline phosphatase (TNAP), an ectoenzyme necessary for mineralization by its ability to hydrolyze inorganic pyrophosphate (PPi), is stimulated by inflammation in vascular smooth muscle cells (VSMCs). Our objective was to determine the role of TNAP in trans-differentiation of VSMCs and calcification...
December 6, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27863377/variable-patterns-of-ectopic-mineralization-in-enpp1asj-2j-mice-a-model-for-generalized-arterial-calcification-of-infancy
#13
Sarah Y Siu, Nathaniel A Dyment, David W Rowe, John P Sundberg, Jouni Uitto, Qiaoli Li
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder characterized by early onset of extensive mineralization of the cardiovascular system. The classical forms of GACI are caused by mutations in the ENPP1 gene, encoding a membrane-bound pyrophosphatase/phosphodiesterase that hydrolyzes ATP to AMP and inorganic pyrophosphate. The asj-2J mouse harboring a spontaneous mutation in the Enpp1 gene has been characterized as a model for GACI. These mutant mice develop ectopic mineralization in skin and vascular connective tissues as well as in cartilage and collagen-rich tendons and ligaments...
December 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27862353/glutaraldehyde-collagen-cross-linking-stabilizes-resin-dentin-interfaces-and-reduces-bond-degradation
#14
Joshua Lee, Camila Sabatini
This study evaluated the stability of resin-dentin interfaces treated with glutaraldehyde-containing agents, and assessed collagen degradation in dentin matrices treated with Gluma. Microtensile bond strength (μTBS) was evaluated 24 h and 6 months after treatment with three desensitizers (Gluma Desensitizer, Gluma Desensitizer Power Gel, and MicroPrime G) and two etch-and-rinse adhesives (Comfort Bond & Desensitizer and iBond TE). Demineralized beams of human dentin were treated with water or Gluma, and the degradation of collagen in these beams was assessed by quantification of the dry mass loss and of the amount of hydroxyproline released from hydrolyzed specimens after 1 or 4 wk...
November 16, 2016: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/27815257/sirt3-blocks-myofibroblast-differentiation-and-pulmonary-fibrosis-by-preventing-mitochondrial-dna-damage
#15
Samik Bindu, Vinodkumar B Pillai, Abhinav Kanwal, Sadhana Samant, Gökhan M Mutlu, Eric Verdin, Nickolai Dulin, Mahesh P Gupta
Myofibroblast differentiation is a key process in the pathogenesis of fibrotic diseases. Transforming growth factor-β1 (TGF-β1) is a powerful inducer of myofibroblast differentiation and is implicated in pathogenesis of tissue fibrosis. This study was undertaken to determine the role of mitochondrial deacetylase SIRT3 in TGF-β1-induced myofibroblast differentiation in vitro and lung fibrosis in vivo. Treatment of human lung fibroblasts with TGF-β1 resulted in increased expression of fibrosis markers, smooth muscle α-actin (α-SMA), collagen-1, and fibronectin...
January 1, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27789968/reduction-of-facial-wrinkles-by-hydrolyzed-water-soluble-egg-membrane-associated-with-reduction-of-free-radical-stress-and-support-of-matrix-production-by-dermal-fibroblasts
#16
Gitte S Jensen, Bijal Shah, Robert Holtz, Ashok Patel, Donald C Lo
OBJECTIVE: The aim of this study was to evaluate the effects of water-soluble egg membrane (WSEM) on wrinkle reduction in a clinical pilot study and to elucidate specific mechanisms of action using primary human immune and dermal cell-based bioassays. METHODS: To evaluate the effects of topical application of WSEM (8%) on human skin, an open-label 8-week study was performed involving 20 healthy females between the age of 45 years and 65 years. High-resolution photography and digital analysis were used to evaluate the wrinkle depth in the facial skin areas beside the eye (crow's feet)...
2016: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/27777467/characterization-of-swiftlet-edible-bird-nest-a-mucin-glycoprotein-and-its-adulterants-by-raman-microspectroscopy
#17
Eric K S Shim, Gleen F Chandra, S Pedireddy, Soo-Y Lee
Edible bird's nest (EBN) is made from the glutinous salivary secretion of highly concentrated mucin glycoprotein by swiftlets (genus Aerodramus or Collocalia) native to the Indo-Pacific region. The unique Raman spectrum of EBN has vibrational lines that can be assigned to peptides and saccharides in the glycoprotein, and it can be used to screen for adulteration. The common edible adulterants classified into two types. Type I adulterants, such as fish bladder, pork skin, karaya gum, coralline seaweed, agar strips, and tremella fungus, were solids which adhered externally on the surface of the EBN cement...
September 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27771442/serum-based-fluorescent-assay-for-evaluating-dipeptidyl-peptidase-i-activity-in-collagen-induced-arthritis-rat-model
#18
Xiaoqian Liu, Jingjing Wang, Yi Chu, Xiaoying Zhou
Dipeptidyl peptidase I (DPPI) is a lysosomal cysteine protease and derived from immune granule cells. It has been suggested playing an important role in the development of rheumatoid arthritis. In this study, a coumarin based fluorescent probe (GF-AFC) was designed and synthesized to evaluate DPPI activity in serum or tissue homogenates of collagen-induced arthritis (CIA) rats, an inflammatory arthropathy model. It was revealed that the fluorescent intensity was significantly increased in a very short time after specific substrate GF-AFC reacted with the DPPI...
October 19, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27722483/dual-component-collagenous-peptide-reactive-oligomer-hydrogels-as-potential-nerve-guidance-materials-from-characterization-to-functionalization
#19
C Kohn, J M Klemens, C Kascholke, N S Murthy, J Kohn, M Brandenburger, M C Hacker
Toward a new generation of improved nerve guidance conduits (NGCs), novel biomaterials are required to address pressing clinical shortcomings in peripheral nerve regeneration (PNR) and to promote biological performance. A dual-component hydrogel system formed by cross-linking reaction between maleic anhydride groups in an oligomeric building block for cross-linking of free amine functionalities in partially hydrolyzed collagen is formulated for continuous processing and NGC fabrication. The influence of the gelation base is optimized for processing from a double syringe delivery system with a static mixer...
October 18, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27662266/the-role-of-three-dimensional-pure-bovine-gelatin-scaffolds-in-tendon-healing-modeling-and-remodeling-an-in-vivo-investigation-with-potential-clinical-value
#20
Ahmad Oryan, Pardis Sharifi, Ali Moshiri, Ian A Silver
AIM OF THE STUDY: Large tendon defects involving extensive tissue loss present complex clinical problems. Surgical reconstruction of such injuries is normally performed by transplanting autogenous and allogenous soft tissues that are expected to remodel to mimic a normal tendon. However, the use of grafts has always been associated with significant limitations. Tissue engineering employing artificial scaffolds may provide acceptable alternatives. Gelatin is a hydrolyzed form of collagen that is bioactive, biodegradable, and biocompatible...
September 23, 2016: Connective Tissue Research
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