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Hydrolyzed Collagen

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https://www.readbyqxmd.com/read/29750291/collagen-peptides-from-soft%C3%A2-shelled-turtle-induce-calpain%C3%A2-1-expression-and-regulate-inflammatory-cytokine-expression-in-hacat-human-skin-keratinocytes
#1
Tetsushi Yamamoto, Saori Nakanishi, Kuniko Mitamura, Atsushi Taga
Collagen peptides (CPs), derived by hydrolyzing collagen with chemicals or enzymes, are often used as functional materials, due to their various bioactivities and high bioavailability. A previous study by our group reported that collagen from soft‑shelled turtle, Pelodiscus sinensis, induces keratinocytes to undergo epithelial‑mesenchymal transition and facilitates wound healing. Therefore, CPs derived from soft‑shelled turtle collagen may have useful effects on the skin. In the present study, the functional effects of CPs on human skin were examined by analyzing CP‑treated human keratinocytes with a shotgun liquid chromatography/mass spectrometry‑based global proteomic approach...
May 8, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29701488/effectiveness-of-a-dietary-supplement-containing-hydrolyzed-collagen-chondroitin-sulfate-and-glucosamine-in-pain-reduction-and-functional-capacity-in-osteoarthritis-patients
#2
Jordi Puigdellivol, Carme Comellas Berenger, Miguel Ángel Pérez Fernández, Juan M Cowalinsky Millán, Christian Carreras Vidal, Inés Gil Gil, Julio Martínez Pagán, Borja Ruiz Nieto, Francisco Jiménez Gómez, Francesc X Comas Figuerola, María E Aguilar Hernández
This observational, open, multicenter clinical trial with a single treatment group aimed to evaluate the effectiveness of a dietary supplement whose main ingredients are hydrolyzed gelatin, chondroitin sulfate, glucosamine sulfate, and devil's claw and bamboo extracts for pain reduction and improvement of functional capacities in patients with osteoarthritis (OA) of the knee and/or hip (REDART study). In all, 130 patients with OA recruited from Spanish hospitals received the dietary supplement for 6 months...
April 27, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29620193/influence-of-the-interaction-between-ac%C3%A2-sdkp-and-ang-ii-on-the-pathogenesis-and-development-of-silicotic-fibrosis
#3
Yi Zhang, Fang Yang, Yan Liu, Hai-Bing Peng, Yu-Cong Geng, Shi-Feng Li, Hong Xu, Li-Yan Zhu, Xiu-Hong Yang, Darrell Brann
N‑acetyl‑seryl‑aspartyl‑lysyl‑proline (Ac‑SDKP) is a natural tetrapeptide that is released from thymosin β4 by prolyl oligopeptides. It is hydrolyzed by the key enzyme of the renin‑angiotensin system, angiotensin‑converting enzyme (ACE). The aim of the present study was to investigate the alterations in Ac‑SDKP and the ACE/angiotensin II (Ang II)/angiotensin II type 1 (AT1) receptor axis and its impact on the pathogenesis and development of silicotic fibrosis. For in vivo studies, a HOPE MED 8050 exposure control apparatus was used to establish different stages of silicosis in a rat model treated with Ac‑SDKP...
March 29, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29475416/red-light-accelerates-the-formation-of-a-human-dermal-equivalent
#4
Anna Cb Oliveira, Thayz Fl Morais, Claudia Bernal, Virginia Ca Martins, Ana Mg Plepis, Priscila Fc Menezes, Janice R Perussi
Development of biomaterials' substitutes and/or equivalents to mimic normal tissue is a current challenge in tissue engineering. Thus, three-dimensional cell culture using type I collagen as a polymeric matrix cell support designed to promote cell proliferation and differentiation was employed to create a dermal equivalent in vitro, as well to evaluate the photobiomodulation using red light. Polymeric matrix cell support was prepared from porcine serous collagen (1.1%) hydrolyzed for 96 h. The biomaterial exhibited porosity of 95%, a median pore of 44 µm and channels with an average distance between the walls of 78 ± 14 µm...
April 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29447281/peptidic-inhibitors-of-insulin-degrading-enzyme-with-potential-for-dermatological-applications-discovered-via-phage-display
#5
Caitlin N Suire, Sarah Nainar, Michael Fazio, Adam G Kreutzer, Tara Paymozd-Yazdi, Caitlyn L Topper, Caroline R Thompson, Malcolm A Leissring
Insulin-degrading enzyme (IDE) is an atypical zinc-metalloendopeptidase that hydrolyzes insulin and other intermediate-sized peptide hormones, many of which are implicated in skin health and wound healing. Pharmacological inhibitors of IDE administered internally have been shown to slow the breakdown of insulin and thereby potentiate insulin action. Given the importance of insulin and other IDE substrates for a variety of dermatological processes, pharmacological inhibitors of IDE suitable for topical applications would be expected to hold significant therapeutic and cosmetic potential...
2018: PloS One
https://www.readbyqxmd.com/read/29411103/ultrastructure-and-biological-function-of-matrix-vesicles-in-bone-mineralization
#6
REVIEW
Tomoka Hasegawa
Bone mineralization is initiated by matrix vesicles, small extracellular vesicles secreted by osteoblasts, inducing the nucleation and subsequent growth of calcium phosphate crystals inside. Although calcium ions (Ca2+ ) are abundant throughout the tissue fluid close to the matrix vesicles, the influx of phosphate ions (PO43- ) into matrix vesicles is a critical process mediated by several enzymes and transporters such as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), ankylosis (ANK), and tissue nonspecific alkaline phosphatase (TNSALP)...
April 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29364561/analysis-of-ntpdase2-in-the-cell-membrane-using-fluorescence-recovery-after-photobleaching-frap
#7
Franciele Cristina Kipper, Alessandra Sayuri Kikuchi Tamajusuku, Darlan Conterno Minussi, José Eduardo Vargas, Ana Maria Oliveira Battastini, Elzbieta Kaczmarek, Simon Christopher Robson, Guido Lenz, Márcia Rosângela Wink
NTPDase2, a member of the CD39/NTPDase family, is an ecto-nucleotidase anchored to the plasma membrane by two transmembrane domains, with a catalytic site facing the extracellular space and preferentially hydrolyzing nucleoside triphosphates. While NTPDase2 is expressed in many cell types, its unique functionality, mobility and dynamics at the cell membrane remain unexplored. We therefore constructed a recombinant NTPDase2 linked to the yellow fluorescent protein (EYFP) to investigate its dynamics by confocal microscopy...
February 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29258896/chelation-by-collagen-in-the-immobilization-of-aspergillus-oryzae-%C3%AE-galactosidase-a-potential-biocatalyst-to-hydrolyze-lactose-by-batch-processes
#8
Adriano Gennari, Francielle Herrmann Mobayed, Giandra Volpato, Claucia Fernanda Volken de Souza
This work is the first study of the immobilization of Aspergillus oryzae β-galactosidase (Gal) on powdered collagen (Col) that had formed a chelate with aluminum (Col-Al-Gal). Other collagen treatments, including those with acetic acid, glutaraldehyde, and a combination of aluminum and glutaraldehyde (Col-Al-Glu-Gal), were also tested. High-yield (superior to 80%) and high-efficiency (superior to 99%) immobilization was obtained for the derivatives Col-Al-Gal and Col-Al-Glu-Gal, even at high protein loads (500-1,000 mg g-1 of support)...
April 1, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29207560/optimization-of-collagenase-production-by-pseudoalteromonas-sp-sjn2-and-application-of-collagenases-in-the-preparation-of-antioxidative-hydrolysates
#9
Xinghao Yang, Xiao Xiao, Dan Liu, Ribang Wu, Cuiling Wu, Jiang Zhang, Jiafeng Huang, Binqiang Liao, Hailun He
Collagenases are the most important group of commercially-produced enzymes. However, even though biological resources are abundant in the sea, very few of these commercially popular enzymes are from marine sources, especially from marine bacteria. We optimized the production of marine collagenases by Pseudoalteromonas sp. SJN2 and investigated the antioxidant activities of the hydrolysates. Media components and culture conditions associated with marine collagenase production by Pseudoalteromonas sp. SJN2 were optimized by statistical methods, namely Plackett-Burman design and response surface methodology (RSM)...
December 4, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29149042/comparative-study-of-biological-activities-of-venom-from-colubrid-snakes-rhabdophis-tigrinus-yamakagashi-and-rhabdophis-lateralis
#10
Yumiko Komori, Toru Hifumi, Akihiko Yamamoto, Atsushi Sakai, Manabu Ato, Kyoko Sawabe, Toshiaki Nikai
Rhabdophis lateralis , a colubrid snake distributed throughout the continent of Asia, has recently undergone taxonomic revisions. Previously, Rhabdophis lateralis was classified as a subspecies of R. tigrinus (Yamakagashi) until 2012, when several genetic differences were discovered which classified this snake as its own species. To elucidate the toxicity of venom from this poorly studied colubrid, various biological activities were compared between the venom from the two snake species. The components of their venom were compared by the elution profiles of reversed-phase HPLC and SDS-PAGE, and gel filtrated fractions were tested for effects on blood coagulation...
November 17, 2017: Toxins
https://www.readbyqxmd.com/read/29146366/validation-and-theoretical-justification-of-an-lc-ms-method-for-the-animal-species-specific-detection-of-gelatin
#11
Anne J Kleinnijenhuis, Frédérique L van Holthoon, Griet Herregods
Collagen is the most abundant protein family in mammals. Commercial edible gelatins are often produced from bovine and porcine skin and bone and consist mainly of partially hydrolyzed collagen type 1. The gelatin industry would benefit from a sensitive and reliable species detection method to unambiguously demonstrate species authenticity of their products. PCR and ELISA could in principle be used for this purpose. However, for gelatin, problems associated with false-positive and false-negative results, inconsistencies and low reactivity of commercially available kits have been observed with regard to ELISA and PCR methods...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29144022/collagen-a-review-on-its-sources-and-potential-cosmetic-applications
#12
REVIEW
María Isabela Avila Rodríguez, Laura G Rodríguez Barroso, Mirna Lorena Sánchez
Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. This molecule is one of the most abundant in many of the living organisms due to its connective role in biological structures. Due to its abundance, strength and its directly proportional relation with skin aging, collagen has gained great interest in the cosmetic industry. It has been established that the collagen fibers are damaged with the pass of time, losing thickness and strength which has been strongly related with skin aging phenomena [Colágeno para todo...
February 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29037731/sequential-extraction-of-gel-forming-proteins-collagen-and-collagen-hydrolysate-from-gutted-silver-carp-hypophthalmichthys-molitrix-a-biorefinery-approach
#13
Mehdi Abdollahi, Masoud Rezaei, Ali Jafarpour, Ingrid Undeland
Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1-15.4%) compared to collagen isolation by acid or pepsin (3.1-5.9%) (p<0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29034640/-preparation-and-antioxidant-activity-detection-of-collagen-peptide-from-cirrhinus-molitorella-skin
#14
Cuiling Wu, Ribang Wu, Dan Liu, Xinghao Yang, Jiang Zhang, Jiafeng Huang, Hailun He
In order to prepare antioxidant peptide through hydrolyzing low-value protein resources with bacterial extracellular proteases and to discover novel proteases, crude extracellular protease from Pseudoalteromonas sp. SHK1-2 was obtained through fermentation which was used to hydrolyze collagen extracted from Cirrhinus molitorella skin. Small peptide fraction was isolated from hydrolysate by ultrafiltration and Sephadex LH-20 size exclusion chromatography and showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (35...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28948019/temperature-dependent-extraction-kinetics-of-hydrolyzed-collagen-from-scales-of-croaker-fish-using-thermal-extraction
#15
Ololade Olatunji, Adetokunbo Denloye
This study investigates the kinetics of hydrolyzed collagen extraction from the scales of the croaker fish (Pseudotolitus elongatus) at temperature ranging from 60°C to 90°C. Extraction was carried out using hydrothermal treatment over a period of 8 hr, during which the mass of hydrolyzed collagen extracted was obtained every hour. The rate order of extraction was temperature-dependent within the times investigated. At 60°C no measurable extraction was achieved, between 70°C and 80°C the extraction was a zero order while at 90°C and 100°C the extraction was a first order process...
September 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28944963/standardized-ileal-digestibility-and-digestible-indispensable-amino-acid-score-of-porcine-and-bovine-hydrolyzates-in-pigs
#16
Yugal Raj Bindari, Helle Nygaard Laerke, Jan Vaerum Nørgaard
BACKGROUND: Using protein hydrolyzates in food and feed ingredients is a growing area of interest. The present study was conducted to determine the standardized ileal digestibility (SID) and to evaluate protein quality of porcine and bovine hydrolyzates. The SID values were determied in pigs, and these values were used to calculate DIAAS values. RESULTS: The SID of crude protein of hydrolyzates of porcine plasma, bovine muscle, bovine collagen, porcine heart, and porcine muscle was 0...
September 25, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28941141/evaluation-of-cutaneous-rejuvenation-associated-with-the-use-of-ortho-silicic-acid-stabilized-by-hydrolyzed-marine-collagen
#17
Célia Luiza Petersen Vitello Kalil, Valéria Campos, Stela Cignachi, Juliana Favaro Izidoro, Clarissa Prieto Herman Reinehr, Christine Chaves
BACKGROUND: Organic silicon plays an important role in dermal structure by promoting neocollagenesis. Thus, the supplementation of silicon in a highly bioavailable form can be used for skin rejuvenation. AIMS: This study aimed to evaluate skin changes associated with the use of ortho-silicic acid stabilized by hydrolyzed collagen. PATIENTS/METHODS: Patients were randomized to receive 600 mg of ortho-silicic acid stabilized by hydrolyzed collagen (group 1, n = 11) or placebo (group 2, n = 11) to be taken 15 minutes before breakfast for 90 days...
September 20, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28929384/roles-of-dietary-glycine-proline-and-hydroxyproline-in-collagen-synthesis-and-animal-growth
#18
REVIEW
Peng Li, Guoyao Wu
Glycine, proline, and hydroxyproline (Hyp) contribute to 57% of total amino acids (AAs) in collagen, which accounts for one-third of proteins in animals. As the most abundant protein in the body, collagen is essential to maintain the normal structure and strength of connective tissue, such as bones, skin, cartilage, and blood vessels. Mammals, birds, and fish can synthesize: (1) glycine from threonine, serine, choline, and Hyp; (2) proline from arginine; and (3) Hyp from proline residues in collagen, in a cell- and tissue-specific manner...
January 2018: Amino Acids
https://www.readbyqxmd.com/read/28864658/pla-2-and-enpp6-may-act-in-concert-to-generate-phosphocholine-from-the-matrix-vesicle-membrane-during-skeletal-mineralization
#19
Alan J Stewart, Darren T K Leong, Colin Farquharson
Mineralization is a key process in the formation of bone and cartilage in vertebrates, involving the deposition of calcium- and phosphate-containing hydroxyapatite (HA) mineral within a collagenous matrix. Inorganic phosphate (Pi ) accumulation within matrix vesicles (MVs) is a fundamental stage in the precipitation of HA, with PHOSPHO1 being identified as the principal enzyme acting to produce Pi PHOSPHO1 is a dual-specific phosphocholine/phosphoethanolamine phosphatase enriched in mineralizing cells and within MVs...
January 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28767072/insights-into-the-mechanisms-involved-in-strong-hemorrhage-and-dermonecrosis-induced-by-atroxlysin-ia-a-pi-class-snake-venom-metalloproteinase
#20
Luciana Aparecida Freitas-de-Sousa, Mônica Colombini, Mônica Lopes-Ferreira, Solange M T Serrano, Ana Maria Moura-da-Silva
Hemorrhage is the most prominent effect of snake venom metalloproteinases (SVMPs) in human envenomation. The capillary injury is a multifactorial effect caused by hydrolysis of the components of the basement membrane (BM). The PI and PIII classes of SVMPs are abundant in viperid venoms and hydrolyze BM components. However, hemorrhage is associated mostly with PIII-class SVMPs that contain non-catalytic domains responsible for the binding of SVMPs to BM proteins, facilitating enzyme accumulation in the tissue and enhancing its catalytic efficiency...
August 2, 2017: Toxins
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