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Hydrolyzed Collagen

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https://www.readbyqxmd.com/read/29149042/comparative-study-of-biological-activities-of-venom-from-colubrid-snakes-rhabdophis-tigrinus-yamakagashi-and-rhabdophis-lateralis
#1
Yumiko Komori, Toru Hifumi, Akihiko Yamamoto, Atsushi Sakai, Manabu Ato, Kyoko Sawabe, Toshiaki Nikai
Rhabdophis lateralis, a colubrid snake distributed throughout the continent of Asia, has recently undergone taxonomic revisions. Previously, Rhabdophis lateralis was classified as a subspecies of R. tigrinus (Yamakagashi) until 2012, when several genetic differences were discovered which classified this snake as its own species. To elucidate the toxicity of venom from this poorly studied colubrid, various biological activities were compared between the venom from the two snake species. The components of their venom were compared by the elution profiles of reversed-phase HPLC and SDS-PAGE, and gel filtrated fractions were tested for effects on blood coagulation...
November 17, 2017: Toxins
https://www.readbyqxmd.com/read/29146366/validation-and-theoretical-justification-of-an-lc-ms-method-for-the-animal-species-specific-detection-of-gelatin
#2
Anne J Kleinnijenhuis, Frédérique L van Holthoon, Griet Herregods
Collagen is the most abundant protein family in mammals. Commercial edible gelatins are often produced from bovine and porcine skin and bone and consist mainly of partially hydrolyzed collagen type 1. The gelatin industry would benefit from a sensitive and reliable species detection method to unambiguously demonstrate species authenticity of their products. PCR and ELISA could in principle be used for this purpose. However, for gelatin, problems associated with false-positive and false-negative results, inconsistencies and low reactivity of commercially available kits have been observed with regard to ELISA and PCR methods...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29144022/collagen-a-review-on-its-sources-and-potential-cosmetic-applications
#3
REVIEW
María Isabela Avila Rodríguez, Laura G Rodríguez Barroso, Mirna Lorena Sánchez
Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. This molecule is one of the most abundant in many of the living organisms due to its connective role in biological structures. Due to its abundance, strength and its directly proportional relation with skin aging, collagen has gained great interest in the cosmetic industry. It has been established that the collagen fibers are damaged with the pass of time, losing thickness and strength which has been strongly related with skin aging phenomena [Colágeno para todo...
November 16, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29037731/sequential-extraction-of-gel-forming-proteins-collagen-and-collagen-hydrolysate-from-gutted-silver-carp-hypophthalmichthys-molitrix-a-biorefinery-approach
#4
Mehdi Abdollahi, Masoud Rezaei, Ali Jafarpour, Ingrid Undeland
Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1-15.4%) compared to collagen isolation by acid or pepsin (3.1-5.9%) (p<0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29034640/-preparation-and-antioxidant-activity-detection-of-collagen-peptide-from-cirrhinus-molitorella-skin
#5
Cuiling Wu, Ribang Wu, Dan Liu, Xinghao Yang, Jiang Zhang, Jiafeng Huang, Hailun He
In order to prepare antioxidant peptide through hydrolyzing low-value protein resources with bacterial extracellular proteases and to discover novel proteases, crude extracellular protease from Pseudoalteromonas sp. SHK1-2 was obtained through fermentation which was used to hydrolyze collagen extracted from Cirrhinus molitorella skin. Small peptide fraction was isolated from hydrolysate by ultrafiltration and Sephadex LH-20 size exclusion chromatography and showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (35...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28948019/temperature-dependent-extraction-kinetics-of-hydrolyzed-collagen-from-scales-of-croaker-fish-using-thermal-extraction
#6
Ololade Olatunji, Adetokunbo Denloye
This study investigates the kinetics of hydrolyzed collagen extraction from the scales of the croaker fish (Pseudotolitus elongatus) at temperature ranging from 60°C to 90°C. Extraction was carried out using hydrothermal treatment over a period of 8 hr, during which the mass of hydrolyzed collagen extracted was obtained every hour. The rate order of extraction was temperature-dependent within the times investigated. At 60°C no measurable extraction was achieved, between 70°C and 80°C the extraction was a zero order while at 90°C and 100°C the extraction was a first order process...
September 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28944963/standardized-ileal-digestibility-and-digestible-indispensable-amino-acid-score-of-porcine-and-bovine-hydrolyzates-in-pigs
#7
Yugal Raj Bindari, Helle Nygaard Laerke, Jan Vaerum Nørgaard
BACKGROUND: Using protein hydrolyzates in food and feed ingredients is a growing area of interest. The present study was conducted to determine the standardized ileal digestibility (SID) and to evaluate protein quality of porcine and bovine hydrolyzates. The SID values were determied in pigs, and these values were used to calculate DIAAS values. RESULTS: The SID of crude protein of hydrolyzates of porcine plasma, bovine muscle, bovine collagen, porcine heart, and porcine muscle was 0...
September 25, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28941141/evaluation-of-cutaneous-rejuvenation-associated-with-the-use-of-ortho-silicic-acid-stabilized-by-hydrolyzed-marine-collagen
#8
Célia Luiza Petersen Vitello Kalil, Valéria Campos, Stela Cignachi, Juliana Favaro Izidoro, Clarissa Prieto Herman Reinehr, Christine Chaves
BACKGROUND: Organic silicon plays an important role in dermal structure by promoting neocollagenesis. Thus, the supplementation of silicon in a highly bioavailable form can be used for skin rejuvenation. AIMS: This study aimed to evaluate skin changes associated with the use of ortho-silicic acid stabilized by hydrolyzed collagen. PATIENTS/METHODS: Patients were randomized to receive 600 mg of ortho-silicic acid stabilized by hydrolyzed collagen (group 1, n = 11) or placebo (group 2, n = 11) to be taken 15 minutes before breakfast for 90 days...
September 20, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28929384/roles-of-dietary-glycine-proline-and-hydroxyproline-in-collagen-synthesis-and-animal-growth
#9
REVIEW
Peng Li, Guoyao Wu
Glycine, proline, and hydroxyproline (Hyp) contribute to 57% of total amino acids (AAs) in collagen, which accounts for one-third of proteins in animals. As the most abundant protein in the body, collagen is essential to maintain the normal structure and strength of connective tissue, such as bones, skin, cartilage, and blood vessels. Mammals, birds, and fish can synthesize: (1) glycine from threonine, serine, choline, and Hyp; (2) proline from arginine; and (3) Hyp from proline residues in collagen, in a cell- and tissue-specific manner...
September 20, 2017: Amino Acids
https://www.readbyqxmd.com/read/28864658/pla2-and-enpp6-may-act-in-concert-to-generate-phosphocholine-from-the-matrix-vesicle-membrane-during-skeletal-mineralization
#10
Alan J Stewart, Darren T K Leong, Colin Farquharson
Mineralization is a key process in the formation of bone and cartilage in vertebrates, involving the deposition of calcium and phosphate containing hydroxyapatite (HA) mineral within a collagenous matrix. Inorganic phosphate (Pi) accumulation within matrix vesicles (MVs) is a fundamental stage in the precipitation of HA, with PHOSPHO1 being identified as the principal enzyme acting to produce Pi PHOSPHO1 is a dual-specific phosphocholine/phosphoethanolamine phosphatase enriched in mineralizing cells and within MVs...
September 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28767072/insights-into-the-mechanisms-involved-in-strong-hemorrhage-and-dermonecrosis-induced-by-atroxlysin-ia-a-pi-class-snake-venom-metalloproteinase
#11
Luciana Aparecida Freitas-de-Sousa, Mônica Colombini, Mônica Lopes-Ferreira, Solange M T Serrano, Ana Maria Moura-da-Silva
Hemorrhage is the most prominent effect of snake venom metalloproteinases (SVMPs) in human envenomation. The capillary injury is a multifactorial effect caused by hydrolysis of the components of the basement membrane (BM). The PI and PIII classes of SVMPs are abundant in viperid venoms and hydrolyze BM components. However, hemorrhage is associated mostly with PIII-class SVMPs that contain non-catalytic domains responsible for the binding of SVMPs to BM proteins, facilitating enzyme accumulation in the tissue and enhancing its catalytic efficiency...
August 2, 2017: Toxins
https://www.readbyqxmd.com/read/28761179/soluble-epoxide-hydrolase-inhibition-protected-against-angiotensin-ii-induced-adventitial-remodeling
#12
Chi Zhou, Jin Huang, Qing Li, Jiali Nie, Xizhen Xu, Dao Wen Wang
Epoxyeicosatrienoic acids (EETs), the metabolites of cytochrome P450 epoxygenases derived from arachidonic acid, exert important biological activities in maintaining cardiovascular homeostasis. Soluble epoxide hydrolase (sEH) hydrolyzes EETs to less biologically active dihydroxyeicosatrienoic acids. However, the effects of sEH inhibition on adventitial remodeling remain inconclusive. In this study, the adventitial remodeling model was established by continuous Ang II infusion for 2 weeks in C57BL/6 J mice, before which sEH inhibitor 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU) was administered by gavage...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743569/structural-characterization-catalytic-kinetic-and-thermodynamic-properties-of-keratinase-from-bacillus-pumilus-fh9
#13
Mohamed A Abdel-Naby, Heba A El-Refai, Mohammad H A Ibrahim
Bacillus pumilus FH9 keratinase was purified to homogeneity with a 59.9% yield through a series of three steps. The purified enzyme was a monomeric protein with a molecular mass around 50kDa and containing 7.3% carbohydrates. The pure B. pumilus FH9 keratinase was optimally active at pH 9.0 and 60°C. The calculated activation energy for keratin hydrolysis was 24.52kJmol(-1) and its temperature quotient (Q10) was 1.19. The calculated values of thermodynamic parameters for keratin hydrolysis were as follows: ΔH*=21...
December 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28715873/subcritical-water-hydrolyzed-fish-collagen-ameliorates-survival-of-endotoxemic-mice-by-inhibiting-hmgb1-release-in-a-ho-1-dependent-manner
#14
Min Young Ahn, Jung Seok Hwang, Sun Ah Ham, Jinwoo Hur, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Sung Gu Han, Han Geuk Seo
To investigate potential mechanisms underlying the bioactivity of hydrolyzed fish collagen, we examined the anti-inflammatory actions of subcritical water-hydrolyzed fish collagen (SWFC) in lipopolysaccharide (LPS)-triggered inflammation and endotoxemia. SWFC markedly inhibited LPS-stimulated release of high mobility group box 1 (HMGB1) in murine RAW264.7 macrophages, along with decreased cytosolic translocation of HMGB1. Both the protein and mRNA levels of heme oxygenase-1 (HO-1) were significantly upregulated in SWFC-treated RAW 264...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28707490/transferon%C3%A2-a-peptide-mixture-with-immunomodulatory-properties-is-not-immunogenic-when-administered-with-various-adjuvants
#15
Sandra Avila, Leslie Muñoz-García, Said Vázquez-Leyva, Nohemí Salinas-Jazmín, Emilio Medina-Rivero, Lenin Pavón, Gabriela Mellado-Sánchez, Rommel Chacón-Salinas, Sergio Estrada-Parra, Luis Vallejo-Castillo, Sonia Mayra Pérez-Tapia
Transferon, a human dialyzable leukocyte extract (hDLE), is a biotherapeutic that comprises a complex mixture of low-molecular-weight peptides (< 10 kDa) and is used to treat diseases with an inflammatory component. Some biotherapeutics, including those composed of peptides, can induce anti-drug antibodies (ADA) that block or diminish their therapeutic effect. Nevertheless, few studies have evaluated peptide-derived drug immunogenicity. In this study, the immunogenicity of Transferon was examined in a murine model during an immunization scheme using the following adjuvants: Al(OH)3, incomplete Freund's adjuvant (IFA), or Titermax Gold...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28678821/a-casein-hydrolysate-based-formulation-attenuates-obesity-and-associated-non-alcoholic-fatty-liver-disease-and-atherosclerosis-in-ldlr-leiden-mice
#16
Marieke H Schoemaker, Robert Kleemann, Martine C Morrison, Joanne Verheij, Kanita Salic, Eric A F van Tol, Teake Kooistra, Peter Y Wielinga
BACKGROUND: Obesity frequently associates with the development of non-alcoholic fatty liver disease (NAFLD) and atherosclerosis. Chronic inflammation in white adipose tissue (WAT) seems to be an important driver of these manifestations. OBJECTIVE: This study investigated a combination of an extensively hydrolyzed casein (eHC), docosahexaenoic acid (DHA), arachidonic acid (ARA), and Lactobacillus Rhamnosus GG (LGG) (together referred to as nutritional ingredients, NI) on the development of obesity, metabolic risk factors, WAT inflammation, NAFLD and atherosclerosis in high-fat diet-fed LDLr-/-...
2017: PloS One
https://www.readbyqxmd.com/read/28677335/substrate-specificity-and-reaction-mechanism-of-human-prolidase
#17
Piotr Wilk, Monika Uehlein, Jacqueline Kalms, Holger Dobbek, Uwe Mueller, Manfred S Weiss
Prolidase is a ubiquitously distributed dipeptidase and the only dipeptidase in humans capable of cleaving the peptide bond preceding the amino acids proline (Pro) or hydroxyproline (Hyp). It is mainly implicated in the degradation of dietary and endogenous proteins. It is also involved in the terminal steps of collagen catabolism by hydrolyzing Pro and Hyp-containing dipeptides. Finally, it is believed to play a role in the regulation of peptidic hormones. Diminished or absent prolidase activity is related to a rare autosomal disease, referred to as prolidase deficiency (PD)...
September 2017: FEBS Journal
https://www.readbyqxmd.com/read/28602994/fish-collagen-peptide-inhibits-the-adipogenic-differentiation-of-preadipocytes-and-ameliorates-obesity-in-high-fat-diet-fed-mice
#18
Eun Ji Lee, Jinwoo Hur, Sun Ah Ham, Yeonji Jo, SangYoon Lee, Mi-Jung Choi, Han Geuk Seo
Bioactivities of fish collagen peptide are now being elucidated in diverse biological systems. Here, we investigated the effect of fish collagen peptide on the adipogenic differentiation of 3T3-L1 preadipocytes and in obese mice fed a high fat diet (HFD). Subcritical water-hydrolyzed fish collagen peptide (SWFCP) significantly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes, which was accompanied by decreased expression of CCAAT-enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2) genes, key regulators of differentiation and maintenance of adipocytes...
November 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28576050/extraction-and-characterization-of-collagen-from-antarctic-and-sub-antarctic-squid-and-its-potential-application-in-hybrid-scaffolds-for-tissue-engineering
#19
Rui C G Coelho, Ana L P Marques, Sara M Oliveira, Gabriela S Diogo, Rogério P Pirraco, Joana Moreira-Silva, José C Xavier, Rui L Reis, Tiago H Silva, João F Mano
Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub-Antarctic squid Illex argentinus as potential collagen sources...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28569344/radiofrequency-treatment-induces-fibroblast-growth-factor-2-expression-and-subsequently-promotes-neocollagenesis-and-neoangiogenesis-in-the-skin-tissue
#20
Patrícia Froes Meyer, Patrícia de Oliveira, Flávia K B A Silva, Ana C S da Costa, Caline R A Pereira, Sebastian Casenave, Rodrigo Marcel Valentim Silva, Luis Gonzaga Araújo-Neto, Sebastião David Santos-Filho, Eric Aizamaque, Hennes Gentil Araújo, Mario Bernardo-Filho, Maria Goretti Freire Carvalho, Ciro Dantas Soares
Radiofrequency (RF) treatment appears to be involved in production of new collagen fibrils and the improvement of existing collagen structures; however, the molecular bases of the effect of non-invasive RF on the skin tissue have not been fully elucidated. This study reports the effects of RF associated or not with hydrolyzed collagen (HC) in the skin tissue. Wistar rats were randomly divided into four groups, according to the treatment received: control group (G1, n = 5), no treatment; subjects in group G2 (n = 5) were treated with HC; and capacitive RF was applied to the back of each subject in G3 (n = 5) and RF associated with HC in G4 (n = 5)...
November 2017: Lasers in Medical Science
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