Dominick J Hellen, Meredith E Fay, David H Lee, Caroline Klindt-Morgan, Ashley Bennett, Kimberly J Pachura, Arash Grakoui, Stacey S Huppert, Paul A Dawson, Wilbur A Lam, Saul J Karpen
The progress of research focused on cholangiocytes and the biliary tree during development and following injury is hindered by limited available quantitative methodologies. Current techniques include two-dimensional standard histological cell-counting approaches, which are rapidly performed error-prone and lack architectural context; or three-dimensional analysis of the biliary tree in opacified livers, which introduce technical issues along with minimal quantitation. The present study aims to fill these quantitative gaps with a supervised machine learning model (BiliQML) able to quantify biliary forms in the liver of anti-Keratin 19 antibody-stained whole slide images...
April 23, 2024: American Journal of Physiology. Gastrointestinal and Liver Physiology