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https://www.readbyqxmd.com/read/29091720/loss-of-the-caenorhabditis-elegans-pocket-protein-lin-35-reveals-muvb-s-innate-function-as-the-repressor-of-dream-target-genes
#1
Paul D Goetsch, Jacob M Garrigues, Susan Strome
The DREAM (Dp/Retinoblastoma(Rb)-like/E2F/MuvB) transcriptional repressor complex acts as a gatekeeper of the mammalian cell cycle by establishing and maintaining cellular quiescence. How DREAM's three functional components, the E2F-DP heterodimer, the Rb-like pocket protein, and the MuvB subcomplex, form and function at target gene promoters remains unknown. The current model invokes that the pocket protein links E2F-DP and MuvB and is essential for gene repression. We tested this model by assessing how the conserved yet less redundant DREAM system in Caenorhabditis elegans is affected by absence of the sole C...
November 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29090984/a-qualitative-study-on-patient-s-perceptions-of-two-types-of-attachments-for-implant-overdentures
#2
Rubens Ferreira de Albuquerque Junior, Marina Pisani, Christophe Bedos, Cláudia Helena Lovato da Silva, Olivier Fromentin
The aim of this qualitative study was to gain a deeper understanding of patient's perceptions of wearing implant retained overdentures with ball-shaped or cylindrical attachment systems. Twenty-two wearers of implant-supported overdentures participated in this qualitative study based on a randomized crossover clinical trial that aimed to compare a cylindrical attachment (Locator, Zest Anchors Inc, Escondido, CA, USA) and a ball attachment (Retentive Anchor, Straumann AG, Basel, Switzerland). In the phase I of the study, group A experienced ball attachments (n=11) and group B Locator attachment (n=11) for one year...
November 1, 2017: Journal of Oral Implantology
https://www.readbyqxmd.com/read/29084295/two-implant-supported-mandibular-overdentures-do-clinical-denture-quality-and-inter-implant-distance-affect-patient-satisfaction
#3
Sara A Alfadda, Mohammad D Al Amri, Amal Al-Ohali, Arwa Al-Hakami, Noura Al-Madhi
PURPOSE: To investigate the following three null hypotheses in patients rehabilitated with a mandibular overdenture supported by two unsplinted implants: (1) patient satisfaction is not related to the clinical quality of the dentures; (2) inter-implant distance (IID) has no effect on the clinical quality of the dentures; and (3) IID does not influence patient satisfaction. MATERIALS AND METHODS: Forty edentulous patients who were rehabilitated with a two-implant-supported mandibular overdenture participated in the study...
November 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/29037033/carboxymethylcellulose-mediates-the-transport-of-carbon-nanotube-magnetite-nanohybrid-aggregates-in-water-saturated-porous-media
#4
Dengjun Wang, Chang Min Park, Arvid Masud, Nirupam Aich, Chunming Su
Carbon-metal oxide nanohybrids (NHs) are increasingly recognized as the next-generation, promising group of nanomaterials for solving emerging environmental issues and challenges. This research, for the first time, systematically explored the transport and retention of carbon nanotube-magnetite (CNT-Fe3O4) NH aggregates in water-saturated porous media under environmentally relevant conditions. A macromolecule modifier, carboxymethylcellulose (CMC), was employed to stabilize the NHs. Our results show that transport of the magnetic CNT-Fe3O4 NHs was lower than that of nonmagnetic CNT due to larger hydrodynamic sizes of NHs (induced by magnetic attraction) and size-dependent retention in porous media...
November 7, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29027707/a-trichosporonales-genome-tree-based-on-27-haploid-and-three-evolutionarily-conserved-natural-hybrid-genomes
#5
Masako Takashima, Sira Sriswasdi, Ri-Ichiroh Manabe, Moriya Ohkuma, Takashi Sugita, Wataru Iwasaki
To construct a backbone tree consisting of basidiomycetous yeasts, draft genome sequences from 25 species of Trichosporonales (Tremellomycetes, Basidiomycota) were generated. In addition to the hybrid genomes of Trichosporon coremiiforme and T. ovoides that we described previously, we identified an interspecies hybrid genome in Cutaneotrichosporon mucoides (formerly T. mucoides). This hybrid genome had a gene retention rate of approximately 55%, and its closest haploid relative was C. dermatis. After constructing the C...
October 13, 2017: Yeast
https://www.readbyqxmd.com/read/28990973/the-issue-of-gadolinium-retained-in-tissues-insights-on-the-role-of-metal-complex-stability-by-comparing-metal-uptake-in-murine-tissues-upon-the-concomitant-administration-of-lanthanum-and-gadolinium-diethylentriamminopentaacetate
#6
Enza Di Gregorio, Giuseppe Ferrauto, Chiara Furlan, Stefania Lanzardo, Raffaele Nuzzi, Eliana Gianolio, Silvio Aime
OBJECTIVES: The aim of the study was to explore the role of the stability of metal complexes in the processes that lead to the metal retention in the brain and other tissues of mice administered with lanthanides-based contrast agents. This issue was tackled by the simultaneous injection of gadolinium (Gd)-diethylentriamminopentaacetate (DTPA) and lanthanum-DTPA, which have the same charge and structure but differ in their thermodynamic stability by 3 orders of magnitude. MATERIALS AND METHODS: A total of 20 healthy BALB/c mice were administered by a single intravenous injection with a dose consisting of 0...
October 3, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28977633/oxidative-stress-rapidly-stabilizes-promoter-proximal-paused-pol-ii-across-the-human-genome
#7
Kyle A Nilson, Christine K Lawson, Nicholas J Mullen, Christopher B Ball, Benjamin M Spector, Jeffery L Meier, David H Price
Oxidative stress has pervasive effects on cells but how they respond transcriptionally upon the initial insult is incompletely understood. We developed a nuclear walk-on assay that semi-globally quantifies nascent transcripts in promoter-proximal paused RNA polymerase II (Pol II). Using this assay in conjunction with ChIP-Seq, in vitro transcription, and a chromatin retention assay, we show that within a minute, hydrogen peroxide causes accumulation of Pol II near promoters and enhancers that can best be explained by a rapid decrease in termination...
August 18, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28953326/scaling-a-waterfall-a-meta-ethnography-of-adolescent-progression-through-the-stages-of-hiv-care-in-sub-saharan-africa
#8
Shannon Williams, Jenny Renju, Ludovica Ghilardi, Alison Wringe
INTRODUCTION: Observational studies have shown considerable attrition among adolescents living with HIV across the "cascade" of HIV care in sub-Saharan Africa, leading to higher mortality rates compared to HIV-infected adults or children. We synthesized evidence from qualitative studies on factors that promote or undermine engagement with HIV services among adolescents living with HIV in sub-Saharan Africa. METHODS: We systematically searched five databases for studies published between 2005 and 2016 that met pre-defined inclusion criteria...
September 15, 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/28912434/food-grade-filler-particles-as-an-alternative-method-to-modify-the-texture-and-stability-of-myofibrillar-gels
#9
Andrew J Gravelle, Shai Barbut, Alejandro G Marangoni
A series of food grade particles were characterized for their potential as fillers in myofibrillar gels. The fillers were separated into (i) hydrophilic, insoluble, crystalline particles and (ii) starch granules. The particles used were microcrystalline cellulose, oat fiber and walnut shell flour, as well as potato and tapioca starches. Crystalline particles increased hardness and decreased recovery properties. Although all of these fillers decreased the T2 relaxation time of water, this was dependent on particle type and size...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28865133/prosthodontic-rehabilitation-of-an-ectodermal-dysplasia-patient-with-implant-telescopic-crown-attachments
#10
Hussain D Alsayed, Nasser M Alqahtani, John A Levon, Dean Morton
Telescopic crown attachments have been successfully used in completely and partially edentulous patients. This type of attachment provides retention, support, and stability with optimal hygiene for the removable dental prosthesis (RDP). With the emergence of CAD/CAM technology, telescopic crown attachments can be virtually designed and milled precisely to ensure a passive fit of the attachment parts and maximal functionality of the RDP. This article reviews both the clinical outcome and prosthodontic rehabilitation of telescopic crown attachments of an edentulous ectodermal dysplasia patient with solitary rigid conical telescopic crown attachments...
September 2, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28858719/liposome-supported-enzymatic-peritoneal-dialysis
#11
Anna Pratsinis, Stefanie Zuercher, Vincent Forster, Eric J Fischer, Paola Luciani, Jean-Christophe Leroux
Compared to hemodialysis, peritoneal dialysis represents a more straightforward and less invasive alternative, though current solutions are not as effective. Herein, the feasibility of liposome-supported enzymatic peritoneal dialysis (LSEPD) is explored to increase the functionality of peritoneal dialysis for the model indication acute alcohol poisoning. Enzyme-loaded liposomes (E-Liposomes) containing alcohol metabolizing enzymes, alcohol oxidase and catalase, are developed and their in vitro and in vivo performances investigated...
August 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28851635/a-novel-polyurethane-nano-zno-matrix-for-immobilization-of-chitinolytic-enzymes-and-optical-sensing-of-chitin
#12
Preety, Vinita Hooda
Purified chitinase from Vigna mungo and N-acetyl β glucosaminidase (NAGase) from Canavalia ensiformis were immobilized on to the novel polyurethane (PU)/zinc oxide nanoparticles (nano ZnO) composite matrix with a conjugation yield of 0.785±0.01mg/cm(2) and 96.19±0.85% retention of specific activity. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) also confirmed the presence of nano ZnO and enzymes on the PU support. Thus synthesized PU/nano ZnO/chitinase/NAGase conjugates were optimized with respect to pH, temperature and substrate concentration and successfully employed for development of an absorbance based optical biosensor for chitin determination in stored wheat grains...
August 26, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28841076/strategic-workforce-planning-for-health-human-resources-a-nursing-case-analysis
#13
Andrea Baumann, Mary Crea-Arsenio, Noori Akhtar-Danesh, Bonnie Fleming-Carroll, Mabel Hunsberger, Margaret Keatings, Michael David Elfassy, Sarah Kratina
Background Health-care organizations provide services in a challenging environment, making the introduction of health human resources initiatives especially critical for safe patient care. Purpose To demonstrate how one specialty hospital in Ontario, Canada, leveraged an employment policy to stabilize its nursing workforce over a six-year period (2007 to 2012). Methods An observational cross-sectional study was conducted in which administrative data were analyzed to compare full-time status and retention of new nurses prepolicy and during the policy...
September 2016: Canadian Journal of Nursing Research, Revue Canadienne de Recherche en Sciences Infirmières
https://www.readbyqxmd.com/read/28810229/photodegradation-of-sulfathiazole-under-simulated-sunlight-kinetics-photo-induced-structural-rearrangement-and-antimicrobial-activities-of-photoproducts
#14
Xi-Zhi Niu, Julie Glady-Croué, Jean-Philippe Croué
Photolysis is a core natural process impacting the fate of some sulfonamide antibiotics in sunlit waters. In this study, sunlight-induced phototransformation of sulfathiazole was investigated. A photolytic quantum yield of 0.079 was obtained in buffered water (pH = 8.0). Different natural organic matter isolates inhibited the photolysis of sulfathiazole by light screening effect. A kinetic model was developed to predict the photodegradation rate of sulfathiazole using the light screening correction factor of the water matrix in the wavelength range of 300-350 nm...
November 1, 2017: Water Research
https://www.readbyqxmd.com/read/28805255/mandibular-complete-denture-versus-single-implant-overdenture-a-systematic-review-of-patient-reported-outcomes
#15
REVIEW
T E Nogueira, D R Dias, C R Leles
The single-implant mandibular overdenture (SIMO) has been proposed as an alternative for edentulous patients who are poorly adapted to their dentures due to low retention and stability of the conventional mandibular complete denture (CD). However, there is a lack of evidence regarding the effectiveness of SIMO, which can be measured by examining patient perception of treatment effects. The aim of this systematic review was to assess the comparative results of CD and SIMO treatments using patient-reported outcome measures...
August 14, 2017: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/28804052/understanding-interactions-of-formerly-incarcerated-hiv-positive-men-and-transgender-women-with-substance-use-treatment-medical-and-criminal-justice-systems
#16
Nina T Harawa, Bita Amani, Jane Rohde Bowers, Jennifer N Sayles, William Cunningham
BACKGROUND: Low levels of medical care engagement have been noted for HIV-positive people leaving systems of incarceration in the United States. Substance misuse frequently co-occurs with criminal justice involvement in individuals who are living with HIV. METHODS: We analyzed data from in-depth interviews with 19 HIV-positive individuals who were currently or formerly incarcerated in order to elucidate challenges faced in accessing care and maintaining HIV treatment regimens when cycling out of (and often back into) custody...
October 2017: International Journal on Drug Policy
https://www.readbyqxmd.com/read/28773819/nature-of-the-electrochemical-properties-of-sulphur-substituted-limn%C3%A2-o%C3%A2-spinel-cathode-material-studied-by-electrochemical-impedance-spectroscopy
#17
Monika Bakierska, Michał Świętosławski, Roman Dziembaj, Marcin Molenda
In this work, nanostructured LiMn₂O₄ (LMO) and LiMn₂O3.99S0.01 (LMOS1) spinel cathode materials were comprehensively investigated in terms of electrochemical properties. For this purpose, electrochemical impedance spectroscopy (EIS) measurements as a function of state of charge (SOC) were conducted on a representative charge and discharge cycle. The changes in the electrochemical performance of the stoichiometric and sulphur-substituted lithium manganese oxide spinels were examined, and suggested explanations for the observed dependencies were given...
August 16, 2016: Materials
https://www.readbyqxmd.com/read/28762520/nanomaterials-as-stationary-phases-and-supports-in-liquid-chromatography
#18
REVIEW
Sandya R Beeram, Elliott Rodriguez, Suresh Doddavenkatanna, Zhao Li, Allegra Pekarek, Darin Peev, Kathryn Goerl, Gianfranco Trovato, Tino Hofmann, David S Hage
The development of various nanomaterials over the last few decades has led to many applications for these materials in liquid chromatography (LC). This review will look at the types of nanomaterials that have been incorporated into LC systems and the applications that have been explored for such systems. A number of carbon-based nanomaterials and inorganic nanomaterials have been considered for use in LC, ranging from carbon nanotubes, fullerenes and nanodiamonds to metal nanoparticles and nanostructures based on silica, alumina, zirconia and titanium dioxide...
October 2017: Electrophoresis
https://www.readbyqxmd.com/read/28744319/-177-lu-pentixather-comprehensive-preclinical-characterization-of-a-first-cxcr4-directed-endoradiotherapeutic-agent
#19
Margret Schottelius, Theresa Osl, Andreas Poschenrieder, Frauke Hoffmann, Seval Beykan, Heribert Hänscheid, Andreas Schirbel, Andreas K Buck, Saskia Kropf, Markus Schwaiger, Ulrich Keller, Michael Lassmann, Hans-Jürgen Wester
Purpose: Based on the clinical relevance of the chemokine receptor 4 (CXCR4) as a molecular target in cancer and on the success of [(68)Ga]pentixafor as an imaging probe for high-contrast visualization of CXCR4-expression, the spectrum of clinical CXCR4-targeting was expanded towards peptide receptor radionuclide therapy (PRRT) by the development of [(177)Lu]pentixather. Experimental design: CXCR4 affinity, binding specificity, hCXCR4 selectivity and internalization efficiency of [(177)Lu]pentixather were evaluated using different human and murine cancer cell lines...
2017: Theranostics
https://www.readbyqxmd.com/read/28728975/continuous-nitrogen-removal-by-a-single-stage-reactor-packed-with-ring-laced-string-medium
#20
Masahiro Tatara, Shu Ishikawa, Yoshiyuki Ueno
The efficiency of nitrogen removal by a partial-nitritation/anammox (PNA) reaction was investigated using a packed-bed reactor in which ring-laced strings were used as the supporting medium. A stable population of PNA microorganisms was established from typical activated sludge, after less than two months of acclimation in the packed-bed reactor, by applying a high nitrogen-loading rate (NLR: 0.53 kg/m(3)/d) and short hydraulic retention time (HRT: 1.8 h). The stability of reactor performance was confirmed in industrial wastewater (IW), demonstrating a nitrogen removal efficiency (NRE) of greater than 77% during 260 days of continuous operation, between 0...
July 17, 2017: Journal of Bioscience and Bioengineering
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