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Yinjuan Wang, Marion Tissot, Gwenaël Rolin, Patrice Muret, Sophie Robin, Jean-Yves Berthon, Li He, Philippe Humbert, Céline Viennet
Primary melanocytes in culture are useful models for studying epidermal pigmentation and efficacy of melanogenic compounds, or developing advanced therapy medicinal products. Cell extraction is an inevitable and critical step in the establishment of cell cultures. Many enzymatic methods for extracting and growing cells derived from human skin, such as melanocytes, are described in literature. They are usually based on two enzymatic steps, Trypsin in combination with Dispase, in order to separate dermis from epidermis and subsequently to provide a suspension of epidermal cells...
March 21, 2018: Cytotechnology
Takuma Wada, Takeshi Fukuda, Masahiro Shimomura, Yuta Inoue, Masaru Kawanishi, Reiko Tasaka, Tomoyo Yasui, Kazuo Ikeda, Toshiyuki Sumi
The standard treatment for locally advanced uterine cervical cancer is concurrent chemoradiotherapy. Successful neoadjuvant chemotherapy (NAC) may reduce tumor size and facilitate a hysterectomy, thereby improving the prognosis for patients with locally advanced cervical cancer. In contrast, unsuccessful NAC may worsen the prognosis because if a hysterectomy is not possible, the change in treatment plan may delay the initiation of core treatment. Therefore, there is a need to identify biomarkers that predict the efficacy of NAC in patients with uterine cervical cancer...
March 2018: Oncology Letters
Andrea Ciavattini, Giovanni Delli Carpini, Lorenzo Moriconi, Nicolò Clemente, Nina Montik, Rosa De Vincenzo, Anna Del Fabro, Monica Buttignol, Caterina Ricci, Francesca Moro, Francesco Sopracordevole
OBJECTIVES: To evaluate cervical regeneration at 6 months following excisional treatment for high-grade cervical intraepithelial neoplasia (CIN), and to investigate the effect of cone dimensions, age of patients and technique of excision on the efficacy of the regeneration process. DESIGN: Prospective observational multicentric study. SETTING: Three tertiary care and research centres. PARTICIPANTS: Among the 197 eligible women of childbearing age, older than 25 years of age, undergoing for the first time a loop electrosurgical excision procedure or carbon dioxide laser cervical excision for a high-grade CIN at the colposcopy-directed cervical punch biopsy, and with a final diagnosis of high-grade CIN, 165 completed the 6-month follow-up and were included in the analysis...
March 19, 2018: BMJ Open
Luciana Ramos Almeida, Patrícia Silva Costa, Andréa M A Nascimento, Mariana Reis, Katharina Oliveira Barros, Luige Biciati Alvim, Alvaro Cantini Nunes, Dulciene Magalhães Queiroz, Gifone Aguiar Rocha, Jacques Robert Nicoli, Sílvia Beleza Moura
Although <i>Lactobacillus </i>species are recognized as normal inhabitants of porcine gastric mucosa, the association of these bacteria with health status or gastric ulcer disease has never been considered. We investigated the bacterial load of <i>Lactobacillus </i>isolated from the antrum, corpus and <i>pars esophagea</i> of stomachs with (n=13) and without (n=10) ulcer of the <i>pars esophagea</i> of slaughtered pigs. We also evaluated in vitro antagonistic properties against typical pathogens of strains isolated from stomachs without ulcer...
March 19, 2018: Canadian Journal of Microbiology
Matthew Laffer, Lindsay Ackerman
A 23-year-old man presented with new-onset pruritic and painful urticarial lesions and targetoid erythematous plaques on both palms, the trunk, and the upper and lower extremities (Figure 1). Additionally, small pustules were discovered on the neck (Figure 2), and there with edematous erythematous vermillion lips with splaying onto the cutaneous lips without ulceration. The patient stated he had had a fever before the eruption, fatigue, chills, myalgias, and sore throat. A chest x-ray was obtained and showed bilateral infiltrates...
2018: Skinmed
Lukas Valihrach, Peter Androvic, Mikael Kubista
Single-cell analysis has become an established method to study cell heterogeneity and for rare cell characterization. Despite the high cost and technical constraints, applications are increasing every year in all fields of biology. Following the trend, there is a tremendous development of tools for single-cell analysis, especially in the RNA sequencing field. Every improvement increases sensitivity and throughput. Collecting a large amount of data also stimulates the development of new approaches for bioinformatic analysis and interpretation...
March 11, 2018: International Journal of Molecular Sciences
Heather Reagin, Etan Marks, Stephen Weis, Joseph Susa
We report a case in which a 43-year-old African American male with medical history of sickle cell disease (SCD) presented with a nonhealing ulcer. Biopsy revealed features of livedoid vasculopathy. Previously, livedoid vasculopathy had only been described in a patient with sickle cell trait, but never in a patient with SCD. Livedoid vasculopathy most commonly affects the distal lower extremities and is characterized by irregular, punched-out, painful ulcers that heal with stellate white scars of atrophie blanche...
February 28, 2018: American Journal of Dermatopathology
Chao-Chun Yang, Tak-Wah Wong, Chih-Hung Lee, Chien-Hui Hong, Chung-Hsing Chang, Feng-Jie Lai, Shang-Hung Lin, Ching-Chi Chi, Tzu-Kai Lin, Hsi Yen, Chin-Han Wu, Hamm-Ming Sheu, Cheng-Che E Lan
BACKGROUND: Currently available topical treatments for actinic keratosis (AK) are associated with substantial side-effects. OBJECTIVES: To evaluate the efficacy and safety of topical SR-T100 gel in treating AK. METHODS: A multicenter, randomized, double-blinded phase III trial was conducted. Patients with at least two clinically visible AK were enrolled and a punch biopsy was performed on one of the AK to confirm the diagnosis. This study consisted of up to 16-week treatment and 8-week post-treatment periods...
March 2, 2018: Journal of Dermatological Science
Senol Tonyali, Deniz Ates, Filiz Akbiyik, Duygu Kankaya, Dilek Baydar, Ali Ergen
BACKGROUND: Interstitial cystitis (IC)/bladder pain syndrome (BPS) is a challenging disease, affecting thousands of people all around the world, especially women. Although there have been numerous theories regarding IC/BPS etiology, the physiopathology of the disease still remains unclear and there is a lack of certain treatment. OBJECTIVES: The aim of the study was to assess the role of nerve fibers and nerve growth factor (NGF) in the etiopathogenesis of IC/BPS symptoms by demonstrating if there is a correlation between urine NGF levels, amount of peripheral nerves in bladder mucosa and symptom severity...
February 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Mohit Parekh, Davide Borroni, Alessandro Ruzza, Hannah J Levis, Stefano Ferrari, Diego Ponzin, Vito Romano
PURPOSE: To compare different Descemet membrane endothelial keratoplasty (DMEK) graft preparation methods. METHODS: Stripping from the trabecular meshwork (M1) using epithelial spatula; stripping by scoring the peripheral endothelium (M2) using Sinskey hook; stripping by punch method (M3) using donor trephine; Submerged hydro-separation (M4); and pneumatic dissection method (M5) were evaluated. Preparation time, costs, endothelial cell loss (ECL) postpreparation, cell death and morphology were compared...
March 9, 2018: Acta Ophthalmologica
Thaís Miranda Xavier de Almeida, Édila Figuerêdo Feitosa Cavalcanti, Alessandra da Silva Freitas, Roberto José Pessoa de Magalhães, Angelo Maiolino, Sandra Regina Torres
OBJECTIVE: To review published data on oral manifestations of multiple myeloma. METHODS: An electronic database search was performed of articles published from 1971 to November 2016 in order to identify studies that reported oral manifestations of patients with multiple myeloma. Case reports and case series with oral manifestations of multiple myeloma in English were included in the study. An additional search was performed of the references of the selected articles...
January 2018: Revista Brasileira de Hematologia e Hemoterapia
Natália B Andriguetti, Roberta Z Hahn, Lilian F Lizot, Suziane Raymundo, Jose L Costa, Kelly F da Cunha, Ramon M M Vilela, Helena M Kluck, Gilberto Schwartsmann, Marina V Antunes, Rafael Linden
BACKGROUND: Paclitaxel (PCT) is a chemotherapeutic drug widely used for the treatment of several types of tumors, and its use is associated with severe adverse events, mainly neurologic and hematopoietic toxicities. The relation between systemic exposure and clinical response to PCT was previously described, making paclitaxel a potential candidate for therapeutic drug monitoring (TDM). The use of dried blood spot (DBS) sampling could allow complex sampling schedules required for TDM of PCT...
March 4, 2018: Clinical Biochemistry
Mary J Reust, Myung Hee Lee, Jenny Xiang, Wei Zhang, Dong Xu, Tatiana Batson, Tuo Zhang, Jennifer A Downs, Kathryn M Dupnik
Obtaining RNA from clinical samples collected in resource-limited settings can be costly and challenging. The goals of this study were to 1) optimize messenger RNA extraction from dried blood spots (DBS) and 2) determine how transcriptomes generated from DBS RNA compared with whole blood RNA isolated from blood collected in Tempus tubes. We studied paired samples collected from eight adults in rural Tanzania. Venous blood was collected on Whatman 903 Protein Saver cards and in tubes with RNA preservation solution...
March 5, 2018: American Journal of Tropical Medicine and Hygiene
Gaik Si Quah, Ian E Ng, Gratian Punch, Arthur J Richardson
No abstract text is available yet for this article.
March 7, 2018: ANZ Journal of Surgery
Atsunori Baba, Shigeto Matsushita, Kasumi Kitayama, Tetsuo Asakura, Hideki Sezutsu, Akihide Tanimoto, Takuro Kanekura
We investigated the effect of silk fibroin (SF) on wound healing in mice. SF or an amorphous SF film (ASFF) prepared from silk produced by the wild-type silkworm Bombyx mori (WT-SF, WT-ASFF) or by transgenic worms that overexpress the Arg-Gly-Asp (RGD) sequence (TG-SF, TG-ASFF) was placed on 5-mm diameter full-thickness skin wounds made by biopsy punch on the back of 8-12 week-old BALB/c mice. Each wound was covered with WT-ASFF and urethane film (UF), TG-ASFF plus UF, or UF alone (control). Wound closure, histological thickness, the area of granulation tissue, and neovascularization were analyzed 4, 8, and 12 days later...
March 4, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Claudia Al-Karawi, Thorsten Cech, Florian Bang, Claudia S Leopold
The aim of the present study was to investigate the tableting behavior of Ibuprofen DC 85 W with special focus on the tablet disintegration time, the tablet crushing strength and the sticking tendency to punch surfaces. To simulate production conditions, tableting was conducted on a rotary press, equipped with three compaction stations. An I-optimal design of experiments was used to analyze the influence of the pre-compaction, the intermediate compaction and the main compaction force on the two responses: tablet disintegration time and crushing strength...
March 2, 2018: Drug Development and Industrial Pharmacy
Leelavathy Budamakuntla, Eswari Loganathan, Sacchidananda A Sarvajnamurthy, H V Nataraj
Introduction and Objectives: The restoration of the epithelium after injury takes place by migration of epithelial cells adjoining a wound or by centrifugal migration from hair follicles. To evaluate the feasibility and potential healing capacity of scalp follicular unit grafts transplanted into the wound bed of chronic leg ulcers. Materials and Methods: Patients with chronic nonhealing ulcers of more than 6 weeks duration were selected for the study. Those with infected ulcers and uncontrolled diabetes were excluded from the study...
October 2017: Journal of Cutaneous and Aesthetic Surgery
Mei Zhao, Jun Zhou, Yuan-Hua Chen, Li Yuan, Man-Man Yuan, Xin-Qiong Zhang, Yan Hu, Huan Yu
The aim of this study was to investigate the effects of folic acid on impaired wound healing in diabetic mice. Male mice were divided into three groups: group 1, the non-diabetic mice (control); group 2, the streptozotocin (STZ)-induced type 1 diabetic mice; and group 3, the diabetic mice that received a daily dose of 3 mg/kg folic acid via oral gavage. Full-thickness excision wounds were created with 8-mm skin biopsy punches. Each wound closure was continuously evaluated until the wound healed up. Wound healing was delayed in diabetic mice compared with the non-diabetic mice...
2018: Journal of Nutritional Science and Vitaminology
Oganes G Oganesyan, Vladimir V Neroev, Arthur A Grdikanyan, Vostan R Getadaryan
PURPOSE: To describe the feasibility of same-day, consecutive use of a single donor cornea in 5 patients with corneal pathology, who required anterior lamellar and endothelial keratoplasties. METHODS: Descemet membrane (DM) with endothelium was completely stripped from a single corneoscleral button and punched out from the endothelial side with a circular, standard punch. Using a custom-made punch for linear cutting of DM, we obtained 4 quarter-grafts (Q-graft)...
February 27, 2018: Cornea
Karl-Heinz Renner, Leonie Manthey
Previous research has shown that humor and self-presentation are linked in several ways. With regard to individual differences, it turned out that gelotophilia (the joy of being laughed at) and katagelasticism (the joy of laughing at others) are substantially associated with the histrionic self-presentation style that is characterized by performing explicit As-If-behaviors (e.g., irony, parodying others) in everyday interactions. By contrast, gelotophobia (the fear of being laughed at) shows a negative correlation with histrionic self-presentation...
2018: Frontiers in Psychology
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