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https://www.readbyqxmd.com/read/28066139/regulation-of-vascular-smooth-muscle-phenotype-by-cross-regulation-of-kr%C3%A3-ppel-like-factors
#1
Jung Min Ha, Sung Ji Yun, Seo Yeon Jin, Hye Sun Lee, Sun Ja Kim, Hwa Kyoung Shin, Sun Sik Bae
Regulation of vascular smooth muscle cell (VSMC) phenotype plays an essential role in many cardiovascular diseases. In the present study, we provide evidence that krüppel-like factor 8 (KLF8) is essential for tumor necrosis factor α (TNFα)-induced phenotypic conversion of VSMC obtained from thoracic aorta from 4-week-old SD rats. Stimulation of the contractile phenotype of VSMCs with TNFα significantly reduced the VSMC marker gene expression and KLF8. The gene expression of KLF8 was blocked by TNFα stimulation in an ERK-dependent manner...
January 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28053695/study-on-hydroxyurea-response-in-hemoglobinopathies-patients-using-genetic-markers-and-liquid-erythroid-cultures
#2
Serena Sclafani, Alice Pecoraro, Veronica Agrigento, Antonio Troia, Rosario Di Maggio, Massimiliano Sacco, Aurelio Maggio, Elena D'Alcamo, Rosalba Di Marzo
Increased expression of fetal hemoglobin (HbF) may ameliorate the clinical course of hemoglobinopathies. Hydroxyurea (HU) is the only inducer approved for the treatment of these diseases able to stimulate HbF production but patients' response is highly variable indicating the utility of the identification of pharmacogenomic biomarkers in order to predict pharmacological treatment efficacy. To date few studies to evaluate the role of genetic determinants in HU response have been conducted showing contradictory results...
November 2, 2016: Hematology Reports
https://www.readbyqxmd.com/read/28032601/characterization-and-phylogenetic-analysis-of-kr%C3%A3-ppel-like-transcription-factor-klf-gene-family-in-tree-shrews-tupaia-belangeri-chinensis
#3
Ming Shao, Guang-Zhe Ge, Wen-Jing Liu, Ji Xiao, Hou-Jun Xia, Yu Fan, Feng Zhao, Bao-Li He, Ceshi Chen
Krüppel-like factors (KLFs) are a family of zinc finger transcription factors regulating embryonic development and diseases. The phylogenetics of KLFs has not been studied in tree shrews, an animal lineage with a closer relationship to primates than rodents. Here, we identified 17 KLFs from Chinese tree shrew (Tupaia belangeri chinensis). KLF proteins are highly conserved among humans, monkeys, rats, mice and tree shrews compared to zebrafish and chickens. The CtBP binding site, Sin3A binding site and nuclear localization signals are largely conserved between tree shrews and human beings...
December 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/28018142/the-role-of-the-ubiquitously-expressed-transcription-factor-sp1-in-tissue-specific-transcriptional-regulation-and-in-disease
#4
REVIEW
Leigh O'Connor, Jane Gilmour, Constanze Bonifer
Sp1 belongs to the 26 member strong Sp/KLF family of transcription factors. It is a paradigm for a ubiquitously expressed transcription factor and is involved in regulating the expression of genes associated with a wide range of cellular processes in mammalian cells. Sp1 can interact with a range of proteins, including other transcription factors, members of the transcription initiation complex and epigenetic regulators, enabling tight regulation of its target genes. In this review, we discuss the mechanisms involved in Sp1-mediated transcriptional regulation, as well as how a ubiquitous transcription factor can be involved in establishing a tissue-specific pattern of gene expression and mechanisms by which its activity may be regulated...
December 2016: Yale Journal of Biology and Medicine
https://www.readbyqxmd.com/read/27992530/anti-inflammatory-effects-of-rosiglitazone-in-obesity-impaired-wound-healing-depend-on-adipocyte-differentiation
#5
Anna Siebert, Itamar Goren, Josef Pfeilschifter, Stefan Frank
Combined diabetes-obesity syndromes severely impair regeneration of acute skin wounds in mouse models. This study assessed the contribution of subcutaneous adipose tissue to exacerbated wound inflammatory conditions. Genetically obese (ob/ob) mice showed an increased expression of positive transcriptional effectors of adipocyte differentiation such as Krüppel-like factor (KLF)-5 and peroxisome proliferator-activated receptor (PPAR)-γ and an associated expression of leptin and fatty acid-binding protein (FABP)-4, but also CXCL2 in isolated subcutaneous fat...
2016: PloS One
https://www.readbyqxmd.com/read/27966782/impact-of-cryopreservation-on-caprine-fetal-adnexa-derived-stem-cells-and-its-evaluation-for-growth-kinetics-phenotypic-characterization-and-wound-healing-potential-in-xenogenic-rat-model
#6
Anjali Somal, Irfan A Bhat, Indu Baiju, Anuj Pratap Singh, Bibhudatta S K Panda, Perumal Arumugam Desingu, Sriti Pandey, Mukesh Kumar Bharti, Amar Pal, G Saikumar, Vikash Chandra, G Taru Sharma
This study was conducted to know the impact of cryopreservation on caprine fetal adnexa derived mesenchymal stem cells (MSCs) on the basic stem cell characteristics. Gravid caprine uteri (2-3 months) were collected from local abattoir to derive {amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) and cord blood (cCB)} MSCs and expanded in vitro. Cells were vitrified at 3(rd) passage (P3) using 10% DMSO. Post thaw viability and cellular properties were assessed. Cells were expanded to determine growth kinetics, tri-lineage differentiation, localization and molecular expression of MSCs and pluripotency markers; thereafter, these cells were transplanted in the full-thickness (2 × 2cm(2) ) rat skin wound to determine their wound healing potential...
December 14, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27933060/regulators-of-tfh-cell-differentiation
#7
REVIEW
Gajendra M Jogdand, Suchitra Mohanty, Satish Devadas
The follicular helper T (Tfh) cells help is critical for activation of B cells, antibody class switching, and germinal center (GC) formation. The Tfh cells are characterized by the expression of CXC chemokine receptor 5 (CXCR5), ICOS, programed death 1 (PD-1), B cell lymphoma 6 (BCL-6), and IL-21. They are involved in clearing infections and are adversely linked with autoimmune diseases and also have a role in viral replication as well as clearance. On the one hand, Tfh cells are generated from naive CD4(+) T cells with sequential steps involving cytokine signaling (IL-21, IL-6, IL-12, activin A), migration, and positioning in the GC by CXCR5, surface receptors (ICOS/ICOSL, signaling lymphocyte activation molecule-associated protein/signaling lymphocyte activation molecule) as well as transcription factor (BCL-6, c-Maf, and signal transducer and activator of transcription 3) signaling and repressor miR155...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27852611/the-critical-role-of-kr%C3%A3-ppel-like-factors-in-kidney-disease
#8
Sandeep K Mallipattu, Chelsea C Estrada, John Cijiang He
Krüppel-like factors (KLFs) are a family of zinc-finger transcription factors critical to mammalian embryonic development, regeneration, and human disease. There is emerging evidence that KLFs play a vital role in key physiological processes in the kidney, ranging from maintenance of glomerular filtration barrier to tubulointerstitial inflammation to progression of kidney fibrosis. Seventeen members of the KLF family have been identified, and several have been well characterized in the kidney. Although they may share some overlap in their downstream targets, their structure and function remain distinct...
November 16, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27848055/knockdown-of-klf11-attenuates-hypoxia-reoxygenation-injury-via-jak2-stat3-signaling-in-h9c2
#9
Yang Li, Xiaojing Shi, Jian Li, Minghui Zhang, Bo Yu
KLF11 is a Krüppel-like factor (KLF) family member, which plays a central role in cardiac hypertrophy and cerebrovascular protection during ischemic insults. However, the roles of KLF11 in hypoxia/reoxygenation (H/R) injury of rat cardiomyocytes H9c2 have not been elucidated. The aim of this study was to evaluate the effects of KLF11 on H/R injury and investigate the molecular mechanisms involved. Here, we found that KLF11 was increased following H/R and reached the highest level with 24 h hypoxia followed by 12 h reoxygenation...
November 15, 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27845069/mechanism-of-corilagin-interference-with-il-13-stat6-signaling-pathways-in-hepatic-alternative-activation-macrophages-in-schistosomiasis-induced-liver-fibrosis-in-mouse-model
#10
Peng Du, Qian Ma, Zhi-De Zhu, Gang Li, Yao Wang, Quan-Qiang Li, Yun-Fei Chen, Zhen-Zhong Shang, Juan Zhang, Lei Zhao
This study tried to find the mechanism of Corilagin interference with interleukin (IL)-13/signal transducer and activator of transcription (STAT) 6 signaling pathways in IL-13-activated liver alternative activation macrophages in schistosomiasis-induced liver fibrosis in Balb/c mice. As a result, IL-13 in serum and the mRNA expression of IL-13 Receptor α1, IL-4 Receptor α and downstream mediators supressor of cytokine signaling (SOCS) 1, Kruppel-like factor (KLF) 4, peroxisome proliferator-activated receptor (PPAR) δ in the liver tissue were significantly inhibited by Corilagin (P<0...
December 15, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27826105/klf7-transfected-schwann-cell-graft-transplantation-promotes-sciatic-nerve-regeneration
#11
Ying Wang, Wen-Yuan Li, Hua Jia, Feng-Guo Zhai, Wen-Rui Qu, Yong-Xia Cheng, Yan-Cui Liu, Ling-Xiao Deng, Su-Fen Guo, Zai-Shun Jin
Our former study demonstrated that Krüppel-like Factor 7 (KLF7) is a transcription factor that stimulates axonal regeneration after peripheral nerve injury. Currently, we used a gene therapy approach to overexpress KLF7 in Schwann cells (SCs) and assessed whether KLF7-transfected SCs graft could promote sciatic nerve regeneration. SCs were transfected by adeno-associated virus 2 (AAV2)-KLF7 in vitro. Mice were allografted by an acellular nerve (ANA) with either an injection of DMEM (ANA group), SCs (ANA+SCs group) or AAV2-KLF7-transfected SCs (ANA+KLF7-SCs group) to assess repair of a sciatic nerve gap...
January 6, 2017: Neuroscience
https://www.readbyqxmd.com/read/27822342/characterization-and-genetic-manipulation-of-primed-stem-cells-into-a-functional-na%C3%A3-ve-state-with-esrrb
#12
Ricardo Antonio Rossello, Andreas Pfenning, Jason T Howard, Ute Hochgeschwender
AIM: To identify differences between primed mouse embryonic stem cells (ESCs) and fully functional naive ESCs; to manipulate primed cells into a naive state. METHODS: We have cultured 3 lines of cells from different mouse strains that have been shown to be naive or primed as determined by generating germline-transmitting chimeras. Cells were put through a battery of tests to measure the different features. RNA from cells was analyzed using microarrays, to determine a priority list of the differentially expressed genes...
October 26, 2016: World Journal of Stem Cells
https://www.readbyqxmd.com/read/27739345/rosuvastatin-inhibits-il-8-and-il-6-production-in-human-coronary-artery-endothelial-cells-stimulated-with-aggregatibacter-actinomycetemcomitans-serotype-b
#13
Diego F Gualtero, Sergio M Viafara-Garcia, Sandra J Morantes, Diana M Buitrago, Octavio A Gonzalez, Gloria I Lafaurie
BACKGROUND: Rosuvastatin exhibits anti-inflammatory effects and reduces periodontal diseases and atherosclerosis; however, its role in regulating periodontopathogen-induced endothelial pro-inflammatory responses remains unclear. The purpose of this study was to determine whether rosuvastatin can reduce the pro-inflammatory response induced by A. actinomycetemcomitans in human coronary artery endothelial cells (HCAECs). METHODS: HCAECs were stimulated with purified A...
October 14, 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/27738243/autotaxin-mediated-lipid-signaling-intersects-with-lif-and-bmp-signaling-to-promote-the-naive-pluripotency-transcription-factor-program
#14
Cody Kime, Masayo Sakaki-Yumoto, Leeanne Goodrich, Yohei Hayashi, Salma Sami, Rik Derynck, Michio Asahi, Barbara Panning, Shinya Yamanaka, Kiichiro Tomoda
Developmental signaling molecules are used for cell fate determination, and understanding how their combinatorial effects produce the variety of cell types in multicellular organisms is a key problem in biology. Here, we demonstrate that the combination of leukemia inhibitory factor (LIF), bone morphogenetic protein 4 (BMP4), lysophosphatidic acid (LPA), and ascorbic acid (AA) efficiently converts mouse primed pluripotent stem cells (PSCs) into naive PSCs. Signaling by the lipid LPA through its receptor LPAR1 and downstream effector Rho-associated protein kinase (ROCK) cooperated with LIF signaling to promote this conversion...
November 1, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27696909/generation-of-induced-pluripotent-stem-cells-with-substitutes-for-yamanaka-s-four-transcription-factors
#15
Xiong Xiao, Nan Li, Dapeng Zhang, Bo Yang, Hongmei Guo, Yuemin Li
Induced pluripotent stem cells (iPSCs) share many characteristics with embryonic stem cells, but lack ethical controversy. They provide vast opportunities for disease modeling, pathogenesis understanding, therapeutic drug development, toxicology, organ synthesis, and treatment of degenerative disease. However, this procedure also has many potential challenges, including a slow generation time, low efficiency, partially reprogrammed colonies, as well as somatic coding mutations in the genome. Pioneered by Shinya Yamanaka's team in 2006, iPSCs were first generated by introducing four transcription factors: Oct 4, Sox 2, Klf 4, and c-Myc (OSKM)...
October 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27611969/rna-seq-analysis-reveals-a-negative-role-of-klf16-in-adipogenesis
#16
Min-Kyung Jang, Sunwoo Lee, Myeong Ho Jung
In this study, we performed high throughput RNA sequencing at the preadipocyte (D0) and differentiated adipocyte (D7) stages of primary brown preadipocyte differentiation in order to characterize the transcriptional events regulating differentiation and function. Compared to the preadipocyte stage (D0), 6,668 genes were identified as differentially expressed genes (DEGs) with a fold change of ≥ 2.0 at the differentiated adipocyte stage (D7). Several adipogenic genes including peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α (C/EBPα), and Krüppel-like factor (KLF) family genes were differentially expressed at D0 and D7...
2016: PloS One
https://www.readbyqxmd.com/read/27602130/hsa-mir-137-hsa-mir-520e-and-hsa-mir-590-3p-perform-crucial-roles-in-lynch-syndrome
#17
Changyu Zhou, Jiayu Li, Jiarui Li, Yingchun Wan, Tao Li, Piyong Ma, Yingjian Wang, Haiyan Sang
The aim of the present study was to identify the differentially expressed microRNAs (DEMs) between Lynch syndrome (LS) and the normal colonic (N-C) control samples, predict the target genes (TGs) and analyze the potential functions of the DEMs and TGs. The miRNA expression dataset GSE30454, which included data of 13 LS and 20 N-C tissue samples, was downloaded from the Gene Expression Omnibus. The classical t-test in Linear Models for Microarray Data package was used for DEM identification. TG prediction was performed using 5 databases...
September 2016: Oncology Letters
https://www.readbyqxmd.com/read/27568462/generation-of-functional-endothelial-cells-with-progenitor-like-features-from-murine-induced-pluripotent-stem-cells
#18
Neli Kachamakova-Trojanowska, Witold Nowak, Krzysztof Szade, Jacek Stepniewski, Karolina Bukowska-Strakova, Monika Zukowska, Hevidar Taha, Antonina Chmura-Skirlinska, Michael Beilharz, Jozef Dulak, Alicja Jozkowicz
Induced pluripotent stem cells (iPSCs) have shown great potential in regenerative medicine and research applications like disease modeling or drug discovery. Endothelium is indispensable for vascular homeostasis, whereas endothelial dysfunction could lead to different diseases. Therefore, generating autologous cells, able to restore the endothelial lining, can be crucial for slowing or reversing certain pathological processes. In the current study we show efficient differentiation of murine iPSCs into endothelial cells (ECs) with stable CD34+/Tie-2+/Sca-1+/CD45- phenotype and proven functionality...
August 25, 2016: Vascular Pharmacology
https://www.readbyqxmd.com/read/27558603/an-improved-reprogrammable-mouse-model-harbouring-the-reverse-tetracycline-controlled-transcriptional-transactivator-3
#19
S Alaei, A S Knaupp, S M Lim, J Chen, M L Holmes, M L Änkö, C M Nefzger, J M Polo
Reprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process...
July 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27540521/the-effect-of-mir-451-upregulation-on-erythroid-lineage-differentiation-of-murine-embryonic-stem-cells
#20
Narges Obeidi, Ali Akbar Pourfathollah, Masoud Soleimani, Mahin Nikougoftar Zarif, Fatemeh Kouhkan
OBJECTIVE: MicroRNAs (miRNAs) are small endogenous non-coding regulatory RNAs that control mRNAs post-transcriptionally. Several mouse stem cells miRNAs are cloned differentially regulated in different hematopoietic lineages, suggesting their possible role in hematopoietic lineage differentiation. Recent studies have shown that specific miRNAs such as Mir-451 have key roles in erythropoiesis. MATERIALS AND METHODS: In this experimental study, murine embryonic stem cells (mESCs) were infected with lentiviruses containing pCDH-Mir-451...
July 2016: Cell Journal
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