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https://www.readbyqxmd.com/read/29775609/kruppel-like-factor-4-klf-4-plays-a-crucial-role-in-simvastatin-svt-induced-differentiation-of-rabbit-articular-chondrocytes
#1
Seon-Mi Yu, Song Ja Kim
Simvastatin is a cholesterol-lowing reagent that is derived synthetically from the fermentation of Aspergillus terreus. Recently, SVT has been shown to possess a protective effect of chondrocytes. Kruppel-like factor 4 (KLF-4) is a zinc finger transcription factor that plays crucial roles during the development and maintenance of multiple organs. However, the roles of KLF-4 in chondrocytes have not been well unknown. Here, we investigated whether KLF-4 regulates SVT-caused differentiated phenotype of chondrocytes...
May 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29739838/overlapping-function-of-klf-family-targets-multiple-transcription-factors-to-maintain-na%C3%A3-ve-pluripotency-of-es-cells
#2
Mariko Yamane, Satoshi Ohtsuka, Kumi Matsuura, Akira Nakamura, Hitoshi Niwa
Krüppel-like factors (Klf) possess a pivotal role to maintain self-renewal of mouse embryonic stem cells (mESCs). The function of three Klf family members has been identified, which are suggested to largely overlap. For further dissection of their functions, we applied an inducible knockout system for these Klf family members and assessed the impacts of combinatorial loss-of-function. As a result, we confirmed that any single Klf family member was sufficient to support self-renewal, whereas the removal of all three compromised it...
May 8, 2018: Development
https://www.readbyqxmd.com/read/29709906/dibenzoylmethane-suppresses-lipid-accumulation-and-reactive-oxygen-species-production-through-regulation-of-nuclear-factor-erythroid-derived-2-like-2-and-insulin-signaling-in-adipocytes
#3
Joo Hyoun Kim, Chae Young Kim, Bobin Kang, Jungil Hong, Hyeon-Son Choi
The aim of this study was to investigate the effects of dibenzoylmethane (1,3-diphenyl-1,3-propanedione, DBM) from licorice roots on lipid accumulation and reactive oxygen species (ROS) production in 3T3-L1 cells. DBM effectively inhibited lipid accumulation during adipogenesis, and its inhibitory effect was shown to be due to the down-regulation of adipogenic factors such as CCAAT-enhancer-binding protein-α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), and fatty acid-binding protein 4 (FABP4)...
2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29625068/chromatin-accessibility-dynamics-during-chemical-induction-of-pluripotency
#4
Shangtao Cao, Shengyong Yu, Dongwei Li, Jing Ye, Xuejie Yang, Chen Li, Xiaoshan Wang, Yuanbang Mai, Yue Qin, Jian Wu, Jiangping He, Chunhua Zhou, He Liu, Bentian Zhao, Xiaodong Shu, Chuman Wu, Ruiping Chen, Waiyee Chan, Guangjin Pan, Jiekai Chen, Jing Liu, Duanqing Pei
Despite its exciting potential, chemical induction of pluripotency (CIP) efficiency remains low and the mechanisms are poorly understood. We report the development of an efficient two-step serum- and replating-free CIP protocol and the associated chromatin accessibility dynamics (CAD) by assay for transposase-accessible chromatin (ATAC)-seq. CIP reorganizes the somatic genome to an intermediate state that is resolved under 2iL condition by re-closing previously opened loci prior to pluripotency acquisition with gradual opening of loci enriched with motifs for the OCT/SOX/KLF families...
April 5, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29587259/kr%C3%A3-ppel-like-factor-4-inhibits-pancreatic-cancer-epithelial-to-mesenchymal-transition-and-metastasis-by-down-regulating-caveolin-1-expression
#5
Zhonglin Zhu, Zhilong Yu, Jianfeng Wang, Lisheng Zhou, Jing Zhang, Bin Yao, Jin Dou, Zhengjun Qiu, Chen Huang
BACKGROUND/AIMS: Krüppel-like factor 4 (KLF4), a member of the KLF family of zinc finger transcription factors, has been identified as a tumor suppressor gene in a variety of tumors. However, the molecular mechanisms by which KLF4 inhibits epithelial-to-mesenchymal transition (EMT) and metastasis in pancreatic cancer remain unclear. METHODS: KLF4 expression in pancreatic cancer was analyzed using public datasets (Oncomine and The Cancer Genome Atlas). The expression of KLF4, caveolin-1 (Cav-1), E-cadherin, and vimentin, and their correlations with clinicopathological characteristics were evaluated by immunohistochemistry in pancreatic cancer tissues...
March 21, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29581309/cooption-of-an-appendage-patterning-gene-cassette-in-the-head-segmentation-of-arachnids
#6
Emily V W Setton, Prashant P Sharma
The jointed appendages of arthropods have facilitated the spectacular diversity and success of this phylum. Key to the regulation of appendage outgrowth is the Krüppel-like factor (KLF)/specificity protein (Sp) family of zinc finger transcription factors. In the fruit fly, Drosophila melanogaster , the Sp6-9 homolog is activated by Wnt-1/wingless ( wg ) and establishes ventral appendage (leg) fate. Subsequently, Sp6-9 maintains expression of the axial patterning gene Distal-less ( Dll ), which promotes limb outgrowth...
March 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29563122/epigenetic-programming-of-t-cells-impacts-immune-reconstitution-in-hematopoietic-stem-cell-transplant-recipients
#7
Kristine Hardy, Corey Smith, Wen Juan Tu, Robert McCuaig, Archana Panikkar, Vijayendra Dasari, Fan Wu, Siok-Keen Tey, Geoffrey R Hill, Rajiv Khanna, Sudha Rao
Immune reconstitution following hematopoietic stem cell transplantation (HSCT) is critical in preventing harmful sequelae in recipients with cytomegalovirus (CMV) infection. To understand the molecular mechanisms underlying immune reconstitution kinetics, we profiled the transcriptome-chromatin accessibility landscape of CMV-specific CD8+ T cells from HCST recipients with different immune reconstitution efficiencies. CMV-specific T cells from HSCT recipients with stable antiviral immunity expressed higher levels of interferon/defense response and cell cycle genes in an interconnected network involving PI3KCG , STAT5B , NFAT , RBPJ , and lower HDAC6 , increasing chromatin accessibility at the enhancer regions of immune and T-cell receptor signaling pathway genes...
March 27, 2018: Blood Advances
https://www.readbyqxmd.com/read/29551501/klf15-promotes-transcription-of-klf3-gene-in-bovine-adipocytes
#8
Hongfang Guo, Rajwali Khan, Sayed Haidar Abbas Raza, Yue Ning, Dawei Wei, Sen Wu, Seyed Mahdi Hosseini, Irfan Ullah, Matthew D Garcia, Linsen Zan
The Krüppel-like factors (KLF) family plays an important role in adipogenesis, which is subject to internal hierarchical regulation. KLF3 is a member of KLF family, mainly responsible for adipocyte differentiation and fat deposition. However, the transcriptional regulation of bovine KLF3 gene and its relationship with KLF15 gene remains unclear during bovine adipogenesis. Here, we report that the expression pattern of KLF3 and KLF15 genes during bovine adipogenesis, when KLF15 gene was overexpressed through adenoviral vector (Ad-KLF15) in bovine adipocytes the expression level of KLF3 gene was increased, similarly when KLF15 was down regulated through siRNA the expression level of KLF3 was also reduced...
June 15, 2018: Gene
https://www.readbyqxmd.com/read/29482257/histone-deacetylase-inhibitor-trapoxin-a-enhances-stemness-properties-in-adipose-tissue-derived-mesenchymal-stem-cells
#9
Leila Mousazadeh, Effat Alizadeh, Nosratollah Zarghami, Shahryar Hashemzadeh, Sedigheh Fekri Aval, Leili Hasanifard, Roya Herizchi
BACK GROUND: Adipose tissue derived mesenchymal stem cells (ASCs) have unique potential for regenerative cell therapies. However, during ex-vivo cultivation, they undergo considerable quality loss regarding their phenotypic properties, stemness genes expression and differentiation potential. Recent studies reported that the loss of stemness properties of MSCs is a result of chromatin histone deacetylations through in-vitro cultivation. The present work aimed to study the effect of Trapoxin A (TPX) as a histone deacetylase inhibitor (HDACi) on overall stemness properties of ASCs...
February 26, 2018: Drug Research
https://www.readbyqxmd.com/read/29480445/clinical-applications-of-induced-pluripotent-stem-cells-stato-attuale
#10
Chavali Kavyasudha, Dannie Macrin, K N ArulJothi, Joel P Joseph, M K Harishankar, Arikketh Devi
In an adult human body, somatic stem cells are present in small amounts in almost all organs with the function of general maintenance and prevention of premature aging. But, these stem cells are not pluripotent and are unable to regenerate large cellular loss caused by infarctions or fractures especially in cells with limited replicative ability such as neurons and cardiomyocytes. These limitations gave rise to the idea of inducing pluripotency to adult somatic cells and thereby restoring their regeneration, replication and plasticity...
February 27, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29467236/hematopoietic-stem-cells-from-induced-pluripotent-stem-cells-considering-the-role-of-microrna-as-a-cell-differentiation-regulator
#11
REVIEW
Aline F Ferreira, George A Calin, Virgínia Picanço-Castro, Simone Kashima, Dimas T Covas, Fabiola A de Castro
Although hematopoietic stem cell (HSC) therapy for hematological diseases can lead to a good outcome from the clinical point of view, the limited number of ideal donors, the comorbidity of patients and the increasing number of elderly patients may limit the application of this therapy. HSCs can be generated from induced pluripotent stem cells (iPSCs), which requires the understanding of the bone marrow and liver niches components and function in vivo iPSCs have been extensively applied in several studies involving disease models, drug screening and cellular replacement therapies...
February 21, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29423105/transcriptome-profiling-identified-differentially-expressed-genes-and-pathways-associated-with-tamoxifen-resistance-in-human-breast-cancer
#12
Xin Men, Jun Ma, Tong Wu, Junyi Pu, Shaojia Wen, Jianfeng Shen, Xun Wang, Yamin Wang, Chao Chen, Penggao Dai
Tamoxifen (TAM) resistance is an important clinical problem in the treatment of breast cancer. In order to identify the mechanism of TAM resistance for estrogen receptor (ER)-positive breast cancer, we screened the transcriptome using RNA-seq and compared the gene expression profiles between the MCF-7 mamma carcinoma cell line and the TAM-resistant cell line TAMR/MCF-7, 52 significant differential expression genes (DEGs) were identified including SLIT2, ROBO, LHX, KLF , VEGFC, BAMBI, LAMA1, FLT4, PNMT, DHRS2, MAOA and ALDH...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29408733/bioinformatics-analysis-of-non-synonymous-variants-in-the-klf-genes-related-to-cardiac-diseases
#13
Katyana Kaline Silva Ferreira, Enéas Ricardo de Morais Gomes, José Luiz de Lima Filho, Carlos Henrique Madeiros Castelletti, Danyelly Bruneska Gondim Martins
Kruppel-like Factors (KLF) are responsible for regulating many genes involved in physiological and pathological processes. They are characterized by three conserved zinc-fingers in the DNA-binding domain, wherein mutations could affect the binding efficiency and transcription regulation. This study aimed to perform bioinformatics analysis to determine the most deleterious non-synonymous variants in KLFs involved in cardiac development and diseases, and their effects over the protein structure and stability...
April 15, 2018: Gene
https://www.readbyqxmd.com/read/29398344/distinct-effect-of-fetal-bovine-serum-versus-follicular-fluid-on-multipotentiality-of-human-granulosa-cells-in-in-vitro-condition
#14
Soudabe Yousefi, Jafar Soleimanirad, Kobra Hamdi, Laya Farzadi, Aalie Ghasemzadeh, Masoumeh Kazemi, Mahdi Mahdipour, Reza Rahbarghazi, Mohammad Nouri
This study aimed to develop an appropriate medium for preservation of multipotentiality in human granulosa cells. To compare the possible effect of different media supplemented with follicular fluid or fetal bovine serum, granulosa cells were cultured in vitro over a period of 14 days. Stemness feature and any alteration in the cell phenotype were monitored using colony count assay and flow cytometry analysis by monitoring the expression of Oct3/4 and GATA-4 factors. Transcript expression level of Sox-2, Klf-4, and Nanog were investigated using quantitative real-time PCR analysis...
March 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29381770/the-conserved-basic-residues-and-the-charged-amino-acid-residues-at-the-%C3%AE-helix-of-the-zinc-finger-motif-regulate-the-nuclear-transport-activity-of-triple-c2h2-zinc-finger-proteins
#15
Chih-Ying Lin, Lih-Yuan Lin
Zinc finger (ZF) motifs on proteins are frequently recognized as a structure for DNA binding. Accumulated reports indicate that ZF motifs contain nuclear localization signal (NLS) to facilitate the transport of ZF proteins into nucleus. We investigated the critical factors that facilitate the nuclear transport of triple C2H2 ZF proteins. Three conserved basic residues (hot spots) were identified among the ZF sequences of triple C2H2 ZF proteins that reportedly have NLS function. Additional basic residues can be found on the α-helix of the ZFs...
2018: PloS One
https://www.readbyqxmd.com/read/29356965/nkg2d-ligands-in-glioma-stem-like-cells-expression-in-situ-and-in-vitro
#16
Charlotte Flüh, Guranda Chitadze, Vivian Adamski, Kirsten Hattermann, Michael Synowitz, Dieter Kabelitz, Janka Held-Feindt
Glioblastoma multiforme (GBM) is a highly malignant brain tumor. Tumor stem cells have a major influence on tumor malignancy, and immunological escape mechanisms, involving the Natural Killer Group 2, member D (NKG2D) receptor-ligand-system, are key elements in tumor immuno-surveillance. We analyzed the expression profile and localization of NKG2D ligands (NKG2DL) and embryonic and neural stem cell markers in solid human GBM and stem-like cells isolated from glioma cell lines by qRT-PCR and immunohistochemistry, including quantitative analysis...
March 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29299121/klf15-promotes-the-proliferation-and-metastasis-of-lung-adenocarcinoma-cells-and-has-potential-as-a-cancer-prognostic-marker
#17
Lihua Gao, Hongmei Qiu, Jian Liu, Yuzhen Ma, Jia Feng, Li Qian, Jianguo Zhang, Yifei Liu, Tingting Bian
Lung adenocarcinoma (LADC)is a general form of non-small cell lung cancer that represents a significant threat to public health worldwide. The 5-year-survival rate for LADC is currently below 15%. The transcription factor KLF15, also called kidney-enriched KLF (KKLF), has been proven to play a role in inhibiting proliferation and diversification of carcinoma cells, including those of the endometrium, pancreas and breast, but the involvement of KLF15 in LADC has not previously been studied. In this study, we compared the in vitro expression of KLF15 in human LADC tissues and adjacent normal lung tissues...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29286255/mir-106b-responsive-gene-landscape-identifies-regulation-of-kruppel-like-factor-family
#18
Cody J Wehrkamp, Sathish Kumar Natarajan, Ashley M Mohr, Mary Anne Phillippi, Justin L Mott
MicroRNA dysregulation is a common feature of cancer and due to the promiscuity of microRNA binding this can result in a wide array of genes whose expression is altered. miR-106b is an oncomiR overexpressed in cholangiocarcinoma and its upregulation in this and other cancers often leads to repression of anti-tumorigenic targets. The goal of this study was to identify the miR-106b-regulated gene landscape in cholangiocarcinoma cells using a genome-wide, unbiased mRNA analysis. Through RNA-Seq we found 112 mRNAs significantly repressed by miR-106b...
December 29, 2017: RNA Biology
https://www.readbyqxmd.com/read/29273682/downregulation-of-decidual-sp1-and-p300-is-associated-with-severe-preeclampsia
#19
Yachao Zhang, Jieqiong Yang, Shijian Lv, Dong-Qin Zhao, Zi-Jiang Chen, Wei-Ping Li, Cong Zhang
Preeclampsia (PE) is a pregnancy-induced disorder characterized by hypertension and proteinuria after 20 weeks of gestation, affecting 5-7% of pregnancies worldwide. So far, the etiology of PE remains poorly understood. Abnormal decidualization is thought to contribute to the development of PE. SP1 belongs to the Sp/KLF superfamily and can recruit P300 to regulate the transcription of several genes. SP1 is also very important for decidualization as it enhances the expression of tissue factor. In this study, we investigated the expression of SP1 and P300 in deciduae and their relationship with PE...
February 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29246575/development-of-one-control-and-one-tumor-specific-induced-pluripotent-stem-cell-line-from-laryngeal-carcinoma-patient
#20
Yamin Zhang, Jing Zhu, Yaozhang Dai, Liuzhong Wang, Rui Liu
Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo...
December 2017: Stem Cell Research
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