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https://www.readbyqxmd.com/read/28067284/oleanolic-acid-ameliorates-high-glucose-induced-endothelial-dysfunction-via-ppar%C3%AE-activation
#1
Zihui Zhang, Manli Jiang, Xinya Xie, Haixia Yang, Xinfeng Wang, Lei Xiao, Nanping Wang
Oleanolic acid (3β-hydroxyolean-12-en-28-oic acid, OA) is a pentacyclic triterpenes widely distributed in food, medicinal plants and nutritional supplements. OA exhibits various pharmacological properties, such as hepatoprotective and anti-tumor effects. In this study, we analyzed the effect of OA on endothelial dysfunction induced by high glucose in human vascular endothelial cells (ECs). Western blotting showed that OA attenuated high glucose-reduced nitric production oxide (NO) as well as Akt-Ser(473) and eNOS-Ser(1177) phosphorylation in cultured human umbilical vein ECs (HUVECs)...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28025449/metformin-enhances-osteogenesis-and-suppresses-adipogenesis-of-human-chorionic-villous-mesenchymal-stem-cells
#2
Qiaoli Gu, Yanzheng Gu, Huilin Yang, Qin Shi
Metformin is the first-line anti-hyperglycemic drugs commonly used to treat type 2 diabetes. Recent studies have shown that metformin can enhance bone formation through induction of endothelial nitric oxide synthase (eNOS). Human chorionic villous mesenchymal stem cells (CV-MSCs) are promising candidates for regenerative medicine. The present study aimed to investigate the effects of metformin on the osteogenic and adipocytic differentiation of human CV-MSCs, and to elucidate the underlying mechanism. CV-MSCs, prepared from human term placentae, were cultured with different concentrations of metformin...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27959936/a-low-dose-of-dietary-quercetin-fails-to-protect-against-the-development-of-an-obese-phenotype-in-mice
#3
Reilly T Enos, Kandy T Velázquez, Meredith S Carson, Jamie L McClellan, Prakash Nagarkatti, Mitzi Nagarkatti, J Mark Davis, E Angela Murphy
The purpose of this study was to examine the effect of a 40% high-fat diet (HFD) supplemented with a dietary attainable level of quercetin (0.02%) on body composition, adipose tissue (AT) inflammation, Non-Alcoholic Fatty-Liver Disease (NAFLD), and metabolic outcomes. Diets were administered for 16 weeks to C57BL/6J mice (n = 10/group) beginning at 4 weeks of age. Body composition and fasting blood glucose, insulin, and total cholesterol concentrations were examined intermittently. AT and liver mRNA expression (RT-PCR) of inflammatory mediators (F4/80, CD206 (AT only), CD11c (AT only) TLR-2 (AT only), TLR-4 (AT only), MCP-1, TNF-α, IL-6 (AT only), and IL-10 (AT only)) were measured along with activation of NFκB-p65, and JNK (western blot)...
2016: PloS One
https://www.readbyqxmd.com/read/27923787/type-of-supplemented-simple-sugar-not-merely-calorie-intake-determines-adverse-effects-on-metabolism-and-aortic-function-in-female-rats
#4
Gemma Sangüesa, Sonali Shaligram, Farjana Akhter, Núria Roglans, Juan C Laguna, Roshanak Rahimian, Marta Alegret
High consumption of simple sugars causes adverse cardio-metabolic effects. We investigated the mechanisms underlying the metabolic and vascular effects of glucose or fructose intake and determined whether these effects are exclusively related to increased calorie consumption. Female Sprague-Dawley rats were supplemented with 20% w/v glucose or fructose for 2 months, and plasma analytes and aortic response to vasodilator and vasoconstrictor agents were determined. Expression of molecules associated with lipid metabolism, insulin signalling and vascular response were evaluated in hepatic and/or aortic tissues...
December 6, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27899720/luteal-function-during-the-estrous-cycle-in-arginine-treated-ewes-fed-different-planes-of-nutrition
#5
Casie S Bass, Dale A Redmer, Samantha L Kaminski, Anna T Grazul-Bilska
Functions of corpus luteum (CL) are influenced by numerous factors including hormones, growth and angiogenic factors, nutritional plane and dietary supplements such as arginine (Arg), a semi-essential amino acid and precursor for proteins, polyamines and nitric oxide (NO). The aim of this study was to determine if Arg supplementation to ewes fed different planes of nutrition influences: (1) progesterone (P4) concentrations in serum and luteal tissue, (2) luteal vascularity, cell proliferation, endothelial NO synthase (eNOS) and receptor (R) soluble guanylate cyclase β protein and mRNA expression and (3) luteal mRNA expression for selected angiogenic factors during the estrous cycle...
March 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27865353/maintenance-of-cytosolic-calcium-is-crucial-to-extend-l-arginine-therapeutic-benefits-during-continuous-dosing
#6
Srinidi Mohan, Lisa Harding
The therapeutic benefits associated with short-term l-arginine supplementation are lost during continuous dosing. AMP-activated protein kinase (AMPK) functional modulation has been correlated with l-arginine therapeutic effectiveness, and with tolerance development during continuous supplementation. However, the metabolic link that is responsible for AMPK functional modulation during continuous l-arginine exposure is currently not known. To explore this, we incubated HUVECs for 7 days with 100 μmol/L l-arginine, in the presence or absence of other agents; and monitored their effects for eNOS function, and on tolerance sparing effects (viz, cellular glucose accumulation, and oxidative stress)...
October 2016: Nutrition Research
https://www.readbyqxmd.com/read/27824430/dietary-fructose-induced-hepatic-injury-in-male-and-female-rats-influence-of-resveratrol
#7
M B Pektas, G Yücel, H B Koca, G Sadi, O G Yıldırım, G Öztürk, F Akar
Purpose: Relatively little is known about gender-dependent susceptibility to hepatic injury induced by nutritional factors. In the current study, we investigated dietary fructose-induced hepatic degeneration and roles of endothelial nitric oxide synthase (eNOS), insulin receptor (IRβ) and substrate-1 (IRS-1) expressions in association with inflammatory markers in male and female rats. Moreover, we examined potential effect of resveratrol on fructose-induced changes. Methods: Male and female rats were divided into 4 groups as control, resveratrol, fructose and resveratrol plus fructose...
November 7, 2016: Drug Research
https://www.readbyqxmd.com/read/27822012/cardioprotective-activity-of-placental-growth-factor-combined-with-oral-supplementation-of-l-arginine-in-a-rat-model-of-acute-myocardial-infarction
#8
Liyun Luo, Bairong Chen, Yin Huang, Zibin Liang, Songbiao Li, Yuelan Yin, Jian Chen, Wei Wu
OBJECTIVE: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with l-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and l-arginine with those of direct administration of PlGF alone in a rat model of AMI. MATERIALS AND METHODS: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, l-arginine group, PlGF group, and combination group (PlGF + l-arginine)...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27722638/attenuation-of-oxidative-stress-in-type-1-diabetic-rats-supplemented-with-a-seasoning-obtained-from-winemaking-by-products-and-its-effect-on-endothelial-function
#9
Raquel Del Pino-García, María D Rivero-Pérez, María L González-SanJosé, Pablo Castilla-Camina, Kevin D Croft, Pilar Muñiz
Type 1 diabetes mellitus (DM) is characterized by hyperglycemia resulting from insulin deficiency. This is usually accompanied by a pro-oxidative environment, dyslipidemia and endothelial dysfunction, thus leading to several micro- and macro-vascular complications. This study investigated the potential benefits of a seasoning obtained from seedless red wine pomace (RWPS) in protecting against oxidative damage and preserving endothelial function in Type 1 DM, and the underlying mechanisms involved at the level of gene expression...
October 12, 2016: Food & Function
https://www.readbyqxmd.com/read/27660232/rice-bran-protein-hydrolysates-reduce-arterial-stiffening-vascular-remodeling-and-oxidative-stress-in-rats-fed-a-high-carbohydrate-and-high-fat-diet
#10
Ketmanee Senaphan, Weerapon Sangartit, Poungrat Pakdeechote, Veerapol Kukongviriyapan, Patchareewan Pannangpetch, Supawan Thawornchinsombut, Stephen E Greenwald, Upa Kukongviriyapan
PURPOSE: Rice bran protein hydrolysates (RBPH) contain highly nutritional proteins and antioxidant compounds which show benefits against metabolic syndrome (MetS). Increased arterial stiffness and the components of MetS have been shown to be associated with an increased risk of cardiovascular disease. This study aimed to investigate whether RBPH could alleviate the metabolic disorders, arterial stiffening, vascular remodeling, and oxidative stress in rats fed a high-carbohydrate and high-fat (HCHF) diet...
September 22, 2016: European Journal of Nutrition
https://www.readbyqxmd.com/read/27614127/salvianolic-acid-b-improves-vascular-endothelial-function-in-diabetic-rats-with-blood-glucose-fluctuations-via-suppression-of-endothelial-cell-apoptosis
#11
Younan Ren, Shanjun Tao, Shuguo Zheng, Mengqiu Zhao, Yuanmei Zhu, Jieren Yang, Yuanjie Wu
Vascular endothelial cell injury is an initial event in atherosclerosis. Salvianolic acid B (Sal B), a main bioactive component in the root of Salvia miltiorrhiza, has vascular protective effect in diabetes, but the underlying mechanisms remain unclear. The present study investigated the effect of Sal B on vascular endothelial function in diabetic rats with blood glucose fluctuations and the possible mechanisms implicated. The results showed that diabetic rats developed marked endothelial dysfunction as exhibited by impaired acetylcholine induced vasodilation...
November 15, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27609225/enhanced-xor-activity-in-enos-deficient-mice-effects-on-the-nitrate-nitrite-no-pathway-and-ros-homeostasis
#12
Maria Peleli, Christa Zollbrecht, Marcelo F Montenegro, Michael Hezel, Jianghong Zhong, Erik G Persson, Rikard Holmdahl, Eddie Weitzberg, Jon O Lundberg, Mattias Carlström
Xanthine oxidoreductase (XOR) is generally known as the final enzyme in purine metabolism and as a source of reactive oxygen species (ROS). In addition, this enzyme has been suggested to mediate nitric oxide (NO) formation via reduction of inorganic nitrate and nitrite. This NO synthase (NOS)-independent pathway for NO generation is of particular importance during certain conditions when NO bioavailability is diminished due to reduced activity of endothelial NOS (eNOS) or increased oxidative stress, including aging and cardiovascular disease...
September 5, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27593859/arginase-inhibition-restores-peroxynitrite-induced-endothelial-dysfunction-via-l-arginine-dependent-endothelial-nitric-oxide-synthase-phosphorylation
#13
Minh Cong Nguyen, Jong Taek Park, Yeong Gwan Jeon, Byeong Hwa Jeon, Kwang Lae Hoe, Young Myeong Kim, Hyun Kyo Lim, Sungwoo Ryoo
PURPOSE: Peroxynitrite plays a critical role in vascular pathophysiology by increasing arginase activity and decreasing endothelial nitric oxide synthase (eNOS) activity. Therefore, the aims of this study were to investigate whether arginase inhibition and L-arginine supplement could restore peroxynitrite-induced endothelial dysfunction and determine the involved mechanism. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with SIN-1, a peroxynitrite generator, and arginase activity, nitrite/nitrate production, and expression levels of proteins were measured...
November 2016: Yonsei Medical Journal
https://www.readbyqxmd.com/read/27564297/improvement-of-erectile-dysfunction-by-the-active-pepide-from-urechis-unicinctus-by-high-temperature-pressure-and-ultra-wave-assisted-lysis-in-streptozotocin-induced-diabetic-rats
#14
Kang Sup Kim, Woong Jin Bae, Su Jin Kim, Kyong-Hwa Kang, Se-Kwon Kim, Hyuk Jin Cho, Sung-Hoo Hong, Ji Youl Lee, Sae Woong Kim
INTRODUCTION: We investigate the effect of active peptide from Urechis unicinctus (UU) by high temperature/pressure and ultra-wave assisted lysis on erectile dysfunction in streptozotocin-induced diabetic rats. MATERIALS AND METHODS: Forty 12-week-old Sprague-Dawley rats were used in this study. Diabetes was induced by a one-time intraperitoneal injection of streptozotocin (50mg/kg). One week later, the diabetic rats were randomly divided into four groups: normal control, untreated diabetes control, and groups treated with 100 or 500mg/kg/d UU peptide...
July 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/27519268/low-dose-dietary-nitrate-improves-endothelial-dysfunction-and-plaque-stability-in-the-apoe-mouse-fed-a-high-fat-diet
#15
J R Bakker, N P Bondonno, T A Gaspari, B K Kemp-Harper, A J McCashney, J M Hodgson, K D Croft, N C Ward
BACKGROUND: Nitric oxide (NO) is an important vascular signalling molecule. NO is synthesised endogenously by endothelial nitric oxide synthase (eNOS). An alternate pathway is exogenous dietary nitrate, which can be converted to nitrite and then stored or further converted to NO and used immediately. Atherosclerosis is associated with endothelial dysfunction and subsequent lesion formation. This is thought to arise due to a reduction in the bioavailability and/or bioactivity of endogenous NO...
August 9, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27499624/endothelial-glycocalyx-conditions-influence-nanoparticle-uptake-for-passive-targeting
#16
Ming J Cheng, Rajiv Kumar, Srinivas Sridhar, Thomas J Webster, Eno E Ebong
Cardiovascular diseases are facilitated by endothelial cell (EC) dysfunction and coincide with EC glycocalyx coat shedding. These diseases may be prevented by delivering medications to affected vascular regions using circulating nanoparticle (NP) drug carriers. The objective of the present study was to observe how the delivery of 10 nm polyethylene glycol-coated gold NPs (PEG-AuNP) to ECs is impacted by glycocalyx structure on the EC surface. Rat fat pad endothelial cells were chosen for their robust glycocalyx, verified by fluorescent immunolabeling of adsorbed albumin and integrated heparan sulfate (HS) chains...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27474450/dietary-nitrate-improves-age-related-hypertension-and-metabolic-abnormalities-in-rats-via-modulation-of-angiotensin-ii-receptor-signaling-and-inhibition-of-superoxide-generation
#17
Michael Hezel, Maria Peleli, Ming Liu, Christa Zollbrecht, Boye L Jensen, Antonio Checa, Alessia Giulietti, Craig E Wheelock, Jon O Lundberg, Eddie Weitzberg, Mattias Carlström
Advanced age is associated with increased risk for cardiovascular disease and type 2 diabetes. A proposed central event is diminished amounts of nitric oxide (NO) due to reduced generation by endothelial NO synthase (eNOS) and increased oxidative stress. In addition, it is widely accepted that increased angiotensin II (ANG II) signaling is also implicated in the pathogenesis of endothelial dysfunction and hypertension by accelerating formation of reactive oxygen species. This study was designed to test the hypothesis that dietary nitrate supplementation could reduce blood pressure and improve glucose tolerance in aged rats, via attenuation of NADPH oxidase activity and ANG II receptor signaling...
July 26, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27465143/%C3%AE-carboxyethyl-hydroxychroman-a-metabolite-of-%C3%AE-tocopherol-preserves-nitric-oxide-bioavailability-in-endothelial-cells-challenged-with-high-glucose
#18
Youyou Li, Leena P Bharath, Ying Qian, Ting Ruan, Pon Velayutham Anandh Babu, Richard S Bruno, J David Symons, Thunder Jalili
Endothelial dysfunction occurs when there are imbalances between factors that regulate the synthesis and degradation of nitric oxide (NO(•)), and has been reported in patients with hyperglycemia and insulin resistance. We reported that supplementation with γ-tocopherol (γ-T) in humans limits impairments in endothelial function otherwise induced by postprandial hyperglycemia. Given the rapid metabolism of γ-T into γ-carboxyethyl hydroxychroman (γ-CEHC), we hypothesized that the vasoprotective activities of γ-T could be attributed to its metabolite γ-CEHC...
July 27, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27450022/the-effects-of-tadalafil-and-pentoxifylline-on-apoptosis-and-nitric-oxide-synthase-in-liver-ischemia-reperfusion-injury
#19
Sibel Bektas, Kemal Karakaya, Murat Can, Burak Bahadir, Berrak Guven, Nilsen Erdogan, Sukru Oguz Ozdamar
The aim of this study was to investigate the effects of tadalafil (TDF) and pentoxifylline (PTX) on hepatic apoptosis and the expressions of endothelial and inducible nitric oxide synthases (eNOS and iNOS) after liver ischemia/reperfusion (IR). Forty Wistar albino rats were randomly divided into five groups (n=8) as follows: sham group; IR group with ischemia/reperfusion alone; low-dose and high-dose TDF groups received 2.5 mg/kg and 10 mg/kg TDF, respectively; and PTX group received 40 mg/kg PTX. Blood was collected for the analysis of serum alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transferase, uric acid, malondialdehyde (MDA), and total antioxidant capacity (TAC)...
July 2016: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/27447401/l-arginine-supplementation-attenuates-capillary-regression-without-increasing-integrated-succinate-dehydrogenase-activity-and-vegf-expression-in-skeletal-muscle-during-hindlimb-unloading
#20
Kensaku Uchida, Minoru Tanaka, Hiroyo Kondo, Akihiko Ishihara, Hidemi Fujino
Decreased capillary number is observed in atrophied muscle. The change in capillary number is regulated by angiogenic factors. L-arginine enhances expression of endothelial nitric oxide synthase (eNOS), angiogenic factor, in skeletal muscle. Therefore, the aim of this study was to evaluate the effects of L-arginine supplementation on capillary regression during hindlimb unloading. Twenty-four male Wistar rats were divided into four treatment groups: (1) control, (2) L-arginine supplementation, (3) hindlimb unloading, and (4) hindlimb unloading with L-arginine supplementation...
October 2016: General Physiology and Biophysics
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