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https://www.readbyqxmd.com/read/28942419/impact-of-point-of-care-management-on-the-transmission-of-anogenital-gonococcal-infections-among-men-who-have-sex-with-men-in-amsterdam-a-mathematical-modelling-and-cost-effectiveness-study
#1
Menne Bartelsman, Kelsey Vaughan, Martijn S van Rooijen, Henry J C de Vries, Maria Xiridou
OBJECTIVE: Point-of-care (POC) management may avert ongoing transmissions occurring between testing and treatment or due to loss to follow-up. We modelled the impact of POC management of anogenital gonorrhoea (with light microscopic evaluation of Gram stained smears) among men who have sex with men (MSM) on gonorrhoea prevalence and testing and treatment costs. METHODS: Data concerning costs and sexual behaviour were collected from the STI clinic of Amsterdam. With a deterministic model for gonorrhoea transmission, we calculated the prevalence of gonorrhoea in MSM in Amsterdam and the numbers of consultations at our clinic over 5 years, in three testing scenarios: POC for symptomatic MSM only (currently routine), POC for all MSM and no POC for MSM...
September 23, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28933563/effects-of-exogenously-applied-salicylic-acid-and-putrescine-alone-and-in-combination-with-rhizobacteria-on-the-phytoremediation-of-heavy-metals-and-chickpea-growth-in-sandy-soil
#2
Naeem Khan, Asghari Bano
The present attempt was made to study the role of exogenously applied salicylic acid (SA) and putrescine (Put) on the phytoremediation of heavy metal and on the growth parameters of chickpea grown in sandy soil. The SA and Put were applied alone as well as in combination with plant growth promoting rhizobacteria (PGPR). The PGPRs, isolated from the rhizosphere of chickpea were characterized on the basis of colony morphology and biochemical traits through gram staining, catalase and oxidase tests, and identified by 16S-rRNA gene sequencing as Bacillus subtilis, Bacillus thuringiensis and Bacillus megaterium...
September 21, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28933325/psychrobacillus-lasiicapitis-sp-nov-isolated-from-the-head-of-an-ant-lasius-fuliginosus
#3
Yibo Shen, Yansong Fu, Yang Yu, Junwei Zhao, Jiansong Li, Yao Li, Xiangjing Wang, Ji Zhang, Wensheng Xiang
A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterium, designated strain NEAU-3TGS17T, was isolated from the head of an ant (Lasius fuliginosus). The isolate grew at 0-35 °C (optimum 28-30 °C), at pH 6.0-11.0 (optimum pH 7.0-8.0) and with 0-6 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-3TGS17T belonged to the genus Psychrobacillus. Sequence similarities between strain NEAU-3TGS17T and members of the genus Psychrobacillus with validly published names (Psychrobacillus psychrotolerans DSM 11706T, Psychrobacillus insolitus DSM 5T, Psychrobacillus psychrodurans DSM 11713T and Psychrobacillus soli NBRC 110600T) were 98...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933324/photobacterium-toruni-sp-nov-a-bacterium-isolated-from-diseased-farmed-fish
#4
Alejandro M Labella, David R Arahal, Teresa Lucena, Manuel Manchado, Dolores Castro, Juan J Borrego
Three bacterial strains were isolated from liver and spleen of diseased farmed redbanded seabream (Pagrus auriga) in south-west Spain. Their partial 16S rRNA gene sequences clustered within those of the genus Photobacterium, showing high similarity (98.6-99.3 %) to the type strains of Photobacterium iliopiscarium, P. piscicola, P. kishitanii, P. aquimaris and P. phosphoreum. Multilocus sequence analysis using six housekeeping genes (gapA, topA, mreB, ftsZ, gyrB and 16S rRNA) confirmed the new strains as forming an independent branch with a bootstrap value of 100, likely to represent a novel species...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933321/dokdonia-lutea-sp-nov-isolated-from-sargassum-fulvellum-seaweed
#5
Seon Choi, Joo Won Kang, Ji Hee Lee, Chi Nam Seong
A non-motile, yellow-coloured and rod-shaped bacterium, designated strain SFD34T, was isolated from seaweed collected from the South Sea, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. Strain SFD34T contained MK-6 and phosphatidylethanolamine as the only isoprenoid quinone and major polar lipid, respectively. The DNA G+C content was 35 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SFD34T formed evolutionary lineage within the radiation enclosing the members of the genus Dokdonia with Dokdoniapacifica SW230T (96...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933319/paracoccus-sordidisoli-sp-nov-isolated-from-an-agricultural-field-contaminated-with-hexachlorocyclohexane-isomers
#6
Amit Kumar Singh, Puneet Kohli, Nitish Kumar Mahato, Rup Lal
A novel bacterial strain, designated LP91T, was isolated from an agricultural field contaminated with hexachlorocyclohexane (HCH) isomers at Ummari Village, Lucknow, Uttar Pradesh, India. Cells of the strain were aerobic, short rod or coccoid, Gram-stain-negative and non-motile. Colonies of the strain were initially transparent but with time changed to a creamy white colour. Phylogenetic analysis based on the 16S rRNA marker gene showed that it was closely associated with Paracoccus aestuariivivens GHD-30T (99...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933318/sphingomonas-antarctica-sp-nov-isolated-from-antarctic-tundra-soil
#7
Yao Huang, Ziyan Wei, Wangmu Danzeng, Myong Chol Kim, Guoxin Zhu, Yumin Zhang, Zuobing Liu, Fang Peng
Strain 200T, isolated from a soil sample taken from Antarctic tundra soil around Zhongshan Station, was found to be a Gram-stain-negative, yellow-pigmented, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain 200T grew optimally at pH 7.0 and in the absence of NaCl on R2A. Its optimum growth temperature was 20 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 200T belonged to the genus Sphingomonas. Strain 200T showed the highest sequence similarities to Sphingomonas kyeonggiense THG-DT81T (95...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933317/elstera-cyanobacteriorum-sp-nov-a-novel-bacterium-isolated-from-cyanobacterial-aggregates-in-a-eutrophic-lake
#8
Haiyuan Cai, Yonghui Zeng, Yanan Wang, Helong Jiang
A bacterial strain, TH019T, was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Cells were observed to be slightly curved rod-shaped and stained Gram-negative. Optimal growth was obtained at pH 7.0 (range, 5.0-9.0) and 28 °C (range, 20-37 °C) in R2A broth. The cells were found to be positive for oxidase and catalase activities, nitrate reduction and denitrification. The major respiratory quinone was ubiquinone Q-10. The major fatty acids were identified as C18 : 1 ω6c/C18 : 1 ω7c and C18 : 1 2OH...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933315/massilia-buxea-sp-nov-isolated-from-a-rock-surface
#9
Le-Ni Sun, En-Dong Yang, Dan-Xi Cui, Ye-Wen Ni, Yu-Bo Wang, Dong-Dong Sun, Wei-Yun Wang
A Gram-stain-negative, rod-shaped and motile bacterial strain, designated A9T, was isolated from the surface of rock collected from the shore of Nvshan lake in Mingguang, Anhui province, China. Phylogenetic analysis based on 16S rDNA sequence data showed that strain A9T was affiliated with the genus Massilia and showed the highest sequence similarities to Massilia plicata KCTC 12344T (98.8 %) and Massilia lurida CGMCC 1.10822T (97.9 %). The major fatty acids (>5 %) were summed feature 3 (C16 : 1ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1ω7c...
September 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28933049/pragmatic-combination-of-available-diagnostic-tools-for-optimal-detection-of-intestinal-microsporidia
#10
Stuti Kaushik, Rumpa Saha, Shukla Das, V G Ramachandran, Ashish Goel
Diarrhea is a debilitating condition in HIV infected individuals and with the finding that almost 1/4 cases of diarrhea in HIV are due to microsporidia, there is a dire need to institute measures for its detection on a regular basis. Keeping this in mind the study aims to determine the burden of intestinal microsporidiosis in HIV seropositive patients presenting with and without diarrhea and to compare the ability of microscopy and PCR in its detection.The study group consisted of 120 patients divided into four groups HIV seropositive with/without diarrhea, and HIV seronegative with/without diarrhea...
September 13, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28930178/diagnostic-value-of-endotracheal-aspirates-sonication-on-ventilator-associated-pneumonia-microbiologic-diagnosis
#11
Laia Fernández-Barat, Ana Motos, Otavio T Ranzani, Gianluigi Li Bassi, Elisabet Aguilera Xiol, Tarek Senussi, Chiara Travierso, Chiara Chiurazzi, Francesco Idone, Laura Muñoz, Jordi Vila, Miquel Ferrer, Paolo Pelosi, Francesco Blasi, Massimo Antonelli, Antoni Torres
Microorganisms are able to form biofilms within respiratory secretions. Methods to disaggregate such biofilms before utilizing standard, rapid, or high throughput diagnostic technologies may aid in pathogen detection during ventilator associated pneumonia (VAP) diagnosis. Our aim was to determine if sonication of endotracheal aspirates (ETA) would increase the sensitivity of qualitative, semi-quantitative, and quantitative bacterial cultures in an animal model of pneumonia caused by Pseudomonasaeruginosa or by methicillin resistant Staphylococcusaureus (MRSA)...
September 20, 2017: Microorganisms
https://www.readbyqxmd.com/read/28928740/microbiome-derived-lipopolysaccharide-enriched-in-the-perinuclear-region-of-alzheimer-s-disease-brain
#12
Yuhai Zhao, Lin Cong, Vivian Jaber, Walter J Lukiw
Abundant clinical, epidemiological, imaging, genetic, molecular, and pathophysiological data together indicate that there occur an unusual inflammatory reaction and a disruption of the innate-immune signaling system in Alzheimer's disease (AD) brain. Despite many years of intense study, the origin and molecular mechanics of these AD-relevant pathogenic signals are still not well understood. Here, we provide evidence that an intensely pro-inflammatory bacterial lipopolysaccharide (LPS), part of a complex mixture of pro-inflammatory neurotoxins arising from abundant Gram-negative bacilli of the human gastrointestinal (GI) tract, are abundant in AD-affected brain neocortex and hippocampus...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28920853/flavobacterium-chuncheonense-sp-nov-and-flavobacterium-luteum-sp-nov-isolated-from-a-freshwater-lake
#13
Miri Park, Gi Gyun Nam, Suhyun Kim, Hyoung Tae Jeon, Yochan Joung, Jang-Cheon Cho
Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920851/loktanella-acticola-sp-nov-isolated-from-seawater
#14
Sooyeon Park, Su Jung Choi, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6T, was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6T fell within the clade comprising the type strains of Loktanella species. Strain OISW-6T exhibited 16S rRNA gene sequence similarity values of 97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920850/ramlibacter-alkalitolerans-sp-nov-alkali-tolerant-bacterium-isolated-from-soil-of-ginseng
#15
Do-Hoon Lee, Chang-Jun Cha
A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showed that it belongs to the genus Ramlibacter within the family Comamonadaceae and was most closely related to Ramlibacter ginsenosidimutans KCTC 22276T (98.1 %), followed by Ramlibacter henchirensis DSM 14656T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920849/flavimarina-flava-sp-nov-isolated-from-salicornia-herbacea
#16
Eui-Sang Cho, In-Tae Cha, Jung-Min Park, Hak-Jong Choi, Jong Hun Lee, Seong Woon Roh, Eun-Ah Cho, Mee-Hyang Kweon, Young-Do Nam, Myung-Ji Seo
A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091T. It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73T with 94.8 % similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920848/dyella-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#17
Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel strain, DKC-1T, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53T (98...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920847/spirosoma-carri-sp-nov-isolated-from-an-automobile-air-conditioning-system
#18
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0406T, was isolated from an automobile evaporator core collected in Korea. The cells were non-motile, aerobic and rod-shaped. The strain grew at 15-37 °C (optimum, 25 °C), at pH 6.0-7.0 (optimum, 6.5) and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetically, the strain was related to members of the genus Spirosoma(93.7-90.7 % 16S rRNA sequence similarities) and showed the highest sequence similarity of 93.7 % to Spirosomapulveris JSH5-14T...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920841/desertibacillus-haloalkaliphilus-gen-nov-sp-nov-isolated-from-a-saline-desert
#19
Hitarth B Bhatt, M Azmatunnisa Begum, Sasikala Chintalapati, Venkata Ramana Chintalapati, Satya P Singh
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920840/flavobacterium-dispersum-sp-nov-isolated-from-a-freshwater-spring
#20
Wen-Ming Chen, Ching-Ling Su, Shih-Yi Sheu
A novel bacterial strain MVW-23T was isolated from a freshwater spring in Taiwan. The strain was Gram-staining-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-30 °C, pH 7.0, and in the presence of 0.5-1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-23T belonged to the genus Flavobacterium and showed the highest levels of sequence similarity with respect to Flavobacterium denitrificans ED5T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
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