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https://www.readbyqxmd.com/read/28550386/human-sperm-toll-like-receptor-4-tlr4-mediates-acrosome-reaction-oxidative-stress-markers-and-sperm-parameters-in-response-to-bacterial-lipopolysaccharide-in-infertile-men
#1
Sana Sahnoun, Afifa Sellami, Nozha Chakroun, Malek Mseddi, Hammadi Attia, Tarek Rebai, Saloua Lassoued
PURPOSE: To study the role of Toll-like receptor 4 (TLR4) in human spermatozoa and to assess sperm parameters, oxidative stress markers, and acrosome reaction in response to the stimulation of TLR4 by its ligand, the lipopolysaccharide (LPS), as a major endotoxin of Gram-negative bacteria. METHODS: Our study was carried out in 73 sperm samples from patients undergoing semen analysis for couple infertility investigations. The studied patients were divided into three groups: normozoospermic fertile patients (n = 13), patients with abnormal and leukospermic semen (n = 13), and patients with abnormal and non-leukospermic semen (n = 47)...
May 26, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28550345/bacillus-zanthoxyli-sp-nov-a-novel-nematicidal-bacterium-isolated-from-chinese-red-pepper-zanthoxylum-bungeanum-maxim-leaves-in-china
#2
Ma Li, Cao Yong Hong, Wang Xiao Yan, Zheng Shuai Chao, Yang Cheng Gang, Duo Jin Ling, Zhou Xing Kui, Xi Jia Qin, Zhu Ming Liang, Mo Ming He
A novel strain, 1433(T), was isolated from leaves of Chinese red pepper (Huajiao, Zanthoxylum bungeanum Maxim) collected from Gansu province in northwestern China, and was characterised by a polyphasic approach. Cells of strain 1433(T) were observed to be Gram-stain positive, aerobic, asporogenous, rod shaped, motile and to have peritrichous flagella. The strain was observed to grow at a range of temperatures and pH, 4-45 °C (optimum 28-32 °C) and 6.0-10.0 (optimum pH 6.0-7.0), respectively. Growth was found to occur in the presence of 0-7% (w/v) NaCl [optimum 0-3% (w/v)]...
May 26, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28549450/the-left-atrial-bacterial-vegetative-mass-due-to-corynebacterium-striatum-as-a-presentation-of-myxoma-a-case-report
#3
Jun Xu, Qing Yang, Jun Li, Xia Zheng
BACKGROUND: Corynebacterium striatum is a member of the non-diphtherial corynebacteria, which are ubiquitous in nature and generally colonize the skin and mucous membranes of humans. Rarely, it causes infective endocarditis (IE). We report a case of rare left atrial bacterial vegetative mass due to C. striatum masquerading as a myxoma identified through a tortuous diagnostic process, and present a brief review of the relevant literature. CASE PRESENTATION: We present a case of 63-year-old man who presented with progressively worsening dyspnea on exertion and lower leg edema, and was diagnosed with heart failure...
May 26, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28546984/the-comparison-of-printed-resources-bacterial-contamination-in-libraries-of-al-zahra-hospital-and-sciences-faculty-of-isfahan-university-and-the-determination-of-their-antibiotic-sensitivity-pattern
#4
Hosein Rafiei, Mostafa Chadeganipour, Rezvan Ojaghi, Mohammad Reza Maracy, Rasool Nouri
INTRODUCTION: During the library loan process, the printed resources can be a carrier of pathogenic bacteria. In this study, it was tried to compare the Bacterial Contamination Rates and their antibiotic sensitivity pattern in printed resources of a hospital and a non-hospital library. METHODS: This is a cross-sectional study. Returning books from the Al-Zahra hospital library and library of Sciences faculty of Isfahan University provides the research community...
2017: Journal of Education and Health Promotion
https://www.readbyqxmd.com/read/28539041/outer-membrane-vesicles-derived-from-salmonella-enteritidis-protect-against-the-virulent-wild-type-strain-infection-in-a-mouse-model
#5
Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu
Food-borne contamination and salmonellosis caused by Salmonella Enteritidis (S. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from S. Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
May 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28537545/paenibacillus-lacus-sp-nov-isolated-from-a-water-reservoir
#6
Wen-Ming Chen, Kai-Rou Lin, Shih-Yi Sheu
A bacterial strain, designated Agd-32T, was isolated from a water sample taken from the Agongdian Reservoir in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain Agd-32T were Gram-stain-variable, aerobic, motile by means of peritrichous flagella, endospore-forming, rod-shaped and surrounded by a thick capsule. Strain Agd-32T contained anteiso-C15 : 0 and C16 : 0 as the predominant fatty acids. The major isoprenoid quinone was MK-7, and the polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophosphoglycolipid, one unidentified glycolipid, one unidentified phospholipid and one unidentified lipid...
May 25, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28534218/flexible-bronchoscopy-in-non-resolving-pneumonia
#7
Javeed Iqbal Bhat, Wasim Ahmad Wani, Qazi Iqbal Ahmad, Bashir Ahmad Charoo, Syed Wajid Ali, Ambreen Ali Ahangar, Muzafar Ahmad
OBJECTIVE: To study the utility of diagnostic flexible bronchoscopy and bronchoalveolar lavage (BAL) in children with non-resolving pneumonia. METHODS: This was a cross-sectional study conducted in a tertiary care hospital from July 2015 through June 2016. Fifty-two consecutive children of both genders from 1 mo to 14 y of age with a diagnosis of non-resolving pneumonia were included. Flexible bronchoscopy was done in all patients with or without bronchoalveolar lavage (BAL)...
May 23, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28534213/impact-of-antimicrobial-stewardship-and-rapid-microarray-testing-on-patients-with-gram-negative-bacteremia
#8
K R Rivard, V Athans, S W Lam, S M Gordon, G W Procop, S S Richter, E Neuner
A rapid microarray assay, Nanosphere Verigene® Gram-negative blood culture test (BC-GN), detects four Gram-negative species, four Gram-negative genera, and six resistance genes directly from positive blood culture samples, shortening the time from Gram stain to pathogen and resistance-gene identification. The purpose of this study was to determine the impact of the BC-GN paired with an antimicrobial stewardship intervention on antimicrobial and clinical outcomes. Patients with Gram-negative bacteremia were compared before (n = 456) and after (n = 421) BC-GN implementation...
May 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28534071/cloning-of-helicobacter-suis-cholesterol-%C3%AE-glucosyltransferase-and-production-of-an-antibody-capable-of-detecting-it-in-formalin-fixed-paraffin-embedded-gastric-tissue-sections
#9
Masatomo Kawakubo, Kazuki Horiuchi, Hitomi Komura, Yoshiko Sato, Masayoshi Kato, Meguru Ikeyama, Mana Fukushima, Shigenori Yamada, Satoshi Ishizone, Takehisa Matsumoto, Hiroyoshi Ota, Junji Sagara, Jun Nakayama
Helicobacter suis (H. suis), formerly called Helicobacter heilmannii type 1 (H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol α-glucosyltransferase (αCgT)...
May 22, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28529273/septicemic-invasive-klebsiella-pneumoniae-infection-in-a-cynomolgus-monkey-macaca-fascicularis-with-severe-diffused-suppurative-meningoencephalitis
#10
Kazufumi Kasuya, Kou Takayama, Makiko Bito, Natsumi Shimokubo, Ryosuke Kawashima, Tomoyuki Shibahara
A 2-year-old male cynomolgus monkey (Macaca fascicularis) showed neurological symptoms during quarantine for importation into Japan, and was euthanized due to poor prognosis. Gross anatomical examination revealed a hemorrhagic lesion around the lateral ventricle in the cerebrum. Histologically, severe diffused suppurative meningitis and ventriculitis were detected with numerous Gram-negative bacilli in the cerebrum. Immunohistochemically, the bacilli were positively stained with an antibody against Klebsiella pneumoniae...
May 22, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529027/management-of-postneurosurgical-meningitis-narrative-review
#11
REVIEW
Khetam Hussein, Roni Bitterman, Ben Shofty, Mical Paul, Ami Neuberger
BACKGROUND: Infections complicating neurosurgery pose unacceptable mortality and morbidity. AIMS: To summarize what is known about the epidemiology, diagnosis and treatment of post-neurosurgical meningitis (PNM). SOURCES: PubMed, references of identified studies and reviews, and personal experience when evidence was lacking. CONTENT: The incidence and pathogen distribution of PNM is highly variable. A shift towards Gram-negative bacteria has been observed with use of antibiotic prophylaxis and antibiotic-coated devices directed mainly against Gram-positive bacteria...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28529026/the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-bacterial-identification-in-patients-with-smear-positive-bacterial-meningitis
#12
Boaz Bishop, Yuval Geffen, Adi Plaut, Ola Kassis, Roni Bitterman, Mical Paul, Ami Neuberger
OBJECTIVES: To assess the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in rapid identification of bacteria from smear-positive cerebrospinal fluid (CSF) in a cohort of patients with meningitis. METHODS: Single-center observational study, including adults and children with community-acquired or postneurosurgical bacterial meningitis. Meningitis was defined using established criteria. CSF samples that had a positive CSF Gram stain were directly examined by MALDI-TOF-MS...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28526569/total-joint-replacement-for-neglected-posterior-knee-dislocation-following-septic-arthritis-after-arthroscopy
#13
Yusuf Öztürkmen, Yunus Emre Akman, Ethem Ayhan Ünkar, Erhan Şükür
This report presents the first case of a knee dislocation following septic arthritis after arthroscopy. A 65-year-old woman had an arthroscopy with irrigation and debridement (I&D) of the joint and microfracture for the chondral lesions. She had complaints of postarthroscopic infection but non-steroidal anti-inflammatory medication and local ice compression was recommended. She revisited her physician twice and at the last visit she had a large purulent effusion in her knee. The gram stain of the joint fluid aspirate demonstrated gram-positive cocci and the cultures grew methicilline-sensitive Staphylococcus aureus...
May 16, 2017: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/28507961/total-hip-arthroplasty-indicated-for-a-tuberculous-coxitis-complicating-a-controlled-acquired-imunodefficiency-syndrom-condition-a-preliminary-report-concerning-a-case
#14
Handy Eone Daniel, Jean Emile Bayiha, Théophyle Chunteng Nana, Olivier Kennedy Muluem, Jean Bahebeck
INTRODUCTION: Total hip arthroplasty (THA) remains controversial in active tuberculosis (TB) infection because of the risks of septic loosening and reactivation of the infection. We present a rare case of THA in a patient positive for the human immunodeficiency virus (HIV) with active tuberculous coxitis. The aim of this work is to share our experience and our preliminary results. CASE REPORT: The patient was a 53-year-old Black African woman, positive for the HIV, who was operated for implantation of a THA via the Hardinge approach indicated for a severe painful hip with restriction of joint movement and limp...
November 2016: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28506793/frequency-and-risk-factors-analysis-of-escherichia-coli-o157-h7-in-bali-cattle
#15
I Wayan Suardana, Dyah Ayu Widiasih, Widagdo Sri Nugroho, Michael Haryadi Wibowo, I Nyoman Suyasa
Cattle are known as the main reservoir of zoonotic agents verocytotoxin-producing Escherichia coli. These bacteria are usually isolated from calves with diarrhea and/or mucus and blood. Tolerance of these agents to the environmental conditions will strengthen of their transmission among livestock. A total of 238 cattle fecal samples from four sub-districts in Badung, Bali were used in this study. Epidemiological data observed include cattle age, sex, cattle rearing system, the source of drinking water, weather, altitude, and type of cage floor, the cleanliness of cage floor, the slope of cage floor, and the level of cattle cleanliness...
May 12, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28501914/skermanella-mucosa-sp-nov-isolated-from-crude-oil-contaminated-soil
#16
Y Subhash, Da-Eun Yoon, Sang-Seob Lee
A novel Gram-stain negative, small rod-shaped bacterium (strain 8-14-6(T)) was isolated from hydrocarbon contaminated desert soil collected from Kuwait. Strain 8-14-6(T) grew at 5-37 °C, pH 6.0-8.8 and 0-2% (w/v) of NaCl concentration. Casein, starch, Tween 20 and Tween 80 were hydrolyzed while urea, chitin, DNA and carboxymethyl-cellulose were not hydrolyzed by strain 8-14-6(T). The major cellular fatty acids were identified as C18:1ω6c/C18:1ω7c, C16:0 and iso-C16:1I/C14:03-OH. Strain 8-14-6(T) produced diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipids, two unidentified lipids and five unidentified amino lipids as polar lipids...
May 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28491909/fluorescent-detection-of-intracellular-nitric-oxide-in-staphylococcus-aureus
#17
April M Lewis, Silvia S Matzdorf, Kelly C Rice
Nitric Oxide (NO) is a highly-reactive radical gas that can modify a variety of cellular targets in both eukaryotes and bacteria. NO is produced endogenously by a wide variety of organisms: For example, as a cell-signaling molecule in mammals and bacteria via nitric oxide synthase (NOS) enzymes, and as a product of denitrification. As such, it is of great benefit to NO researchers to be able to sensitively detect intracellular NO and stable reactive nitrogen species (RNS) derived from NO. To this end, a protocol for fluorescent detection of intracellular NO/RNS in biofilm cultures of the Gram-positive pathogen Staphylococcus aureus has been optimized using the commercially-available cell-permeable fluorescent stain 4-Amino-5-Methylamino-2', 7'-Difluorofluorescein Diacetate (DAF-FM diacetate)...
July 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28490491/comparison-of-the-vitek-ms-and-bruker-maldi-tof-ms-mass-spectrometry-systems-for-the-identification-of-rhodococcus-equi-and-dietzia-spp
#18
Carlos Ruiz de Alegría Puig, Lilian Pilares, Francesc Marco, Jordi Vila, Luis Martínez-Martínez, Jesús Navas
Rhodococcus equi causes pyogranulomatous pneumonia in domesticated animals and immunocompromised humans. Dietzia spp. are environmental bacteria that have been rarely associated with human infections. R. equi and Dietzia spp. are closely related actinomycetes. Phenotypic discrimination between R. equi and Dietzia on the basis of their Gram stain morphology and colony appearance is problematic. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast, reliable and cost-effective method for identification of a wide variety of microorganisms...
May 10, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28490486/meatal-swabs-contain-less-cellular-material-and-are-associated-with-a-decrease-in-gram-stain-smear-quality-compared-to-urethral-swabs-in-men
#19
Stephen J Jordan, Jane R Schwebke, Kristal J Aaron, Barbara Van Der Pol, Edward W Hook
Urethral swabs are the test of choice for point-of-care Gram stain testing to diagnose Neisseria gonorrhoeae (NG) and non-gonococcal urethritis (NGU) in men. As an alternative to urethral swabs, meatal swabs have been recommended for collection of urethral discharge to diagnose NG and Chlamydia trachomatis (CT) in certain populations by nucleic acid amplification testing (NAAT), as they are a less invasive collection method. However, as meatal swabs could be sampling a reduced surface area and result in fewer collected epithelial cells when compared to urethral swabs, the adequacy of meatal swab specimens to collect sufficient cellular material for Gram stain testing remains unknown...
May 10, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28484437/a-novel-raym_rs09735-raym_rs09740-two-component-signaling-system-regulates-gene-expression-and-virulence-in-riemerella-anatipestifer
#20
Ying Wang, Ti Lu, Xuehuan Yin, Zutao Zhou, Shaowen Li, Mei Liu, Sishun Hu, Dingren Bi, Zili Li
The Gram-negative bacterium Riemerella anatipestifer is an important waterfowl pathogen, causing major economic losses to the duck-producing industry. However, little is known of the virulence factors that mediate pathogenesis during R. anatipestifer infection. In this study, RAYM_RS09735 and RAYM_RS09740 were predicted to form a two-component signaling system (TCS) through bioinformatics analysis. This TCS was highly conserved across the Flavobacteriaceae. A mutant YMΔRS09735/RS09740 strain was constructed to investigate the role of the RAYM_RS09735/RAYM_RS09740 TCS in R...
2017: Frontiers in Microbiology
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