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Gram stain

Ram Hari Dahal, Jaisoo Kim
A novel casein hydrolysing bacterium designated strain S-52T was isolated from Kyonggi University forest soil. Cells were strictly aerobic, Gram stain-negative, oxidase- and catalase- positive, non-motile, non-spore-forming, rod-shaped, and golden-yellow-pigmented. Strain S-52T hydrolysed casein. It was able to grow at 20-37 °C (optimum 25-32 °C), pH 6.5-11.0 (optimum 7-9.5), and at 3% (w/v) NaCl concentration. Strain exhibits flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain S-52T formed a lineage within the genus Chitinophaga that was distinct from other species of the genus...
January 19, 2018: Archives of Microbiology
Shan Ye, Lei Jiang, Jimin Wu, Chen Su, Chaobo Huang, Xiufeng Liu, Wei Shao
In this study, we report the design and fabrication of a novel biocompatible sponge with excellent antibacterial property, making it a promising material for wound dressings. The sponge is formed by grafting amoxicillin onto regenerated bacterial cellulose (RBC). It was observed that the grafted RBC could enhance the antibacterial activity against fungus, Gram-negative and Gram-positive bacteria. The morphology of strains treated with the grafted RBC and fluorescent stain results further demonstrated the antibacterial ability of the fabricated sponge...
January 18, 2018: ACS Applied Materials & Interfaces
Sunit Singhi, Suresh Kumar Angurana
CNS infections in children are medical emergency and are associated with high mortality and morbidity. For diagnosis, a high index of suspicion is required. Clinical assessment should be supplemented by laboratory investigations including CSF Gram stain and cultures, blood culture, PCR on CSF, serological tests, and imaging. Commonly associated life threatening complications include coma, seizure, raised intracranial pressure (ICP), focal deficits, shock, respiratory failure, and fluid and electrolyte abnormalities...
January 15, 2018: Indian Journal of Pediatrics
Diogo N Proença, William B Whitman, Neha Varghese, Nicole Shapiro, Tanja Woyke, Nikos C Kyrpides, Paula V Morais
Bacterial strain B29T1T was isolated from the endophytic microbial community of a Pinus pinaster tree trunk and characterized. Strain B29T1T stained Gram-negative and formed diplococci that grew optimally at 26-30°C and at pH 6.0-7.0. The G+C content of the DNA was 61.6mol%. The respiratory quinone was ubiquinone 10 (UK-10), and the major fatty acids were C16:0, cyclo-C19:0ω8c and C18:0 12-methyl, representing 64% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain B29T1T within the order Rhodospirillales in a distinct lineage that also included the genus Geminicoccus...
December 22, 2017: Systematic and Applied Microbiology
Jongkon Saising, Minh Thu Nguyen, Thomas Härtner, Patrick Ebner, Abdulla Al Mamun Bhuyan, Anne Berscheid, Melanie Muehlenkamp, Sina Schäkermann, Nimerta Kumari, Martin E Maier, Supayang P Voravuthikunchai, Julia Bandow, Florian Lang, Heike Brötz-Oesterhelt, Friedrich Götz
Particularly in Asia medicinal plants with antimicrobial activity are used for therapeutic purpose. One such plant-derived antibiotic is rhodomyrtone (Rom) isolated from Rhodomyrtus tomentosa leaves. Rom shows high antibacterial activity against a wide range of Gram-positive bacteria, however, its mode of action is still unclear. Reporter gene assays and proteomic profiling experiments in Bacillus subtilis indicate that Rom does not address classical antibiotic targets like translation, transcription or DNA replication, but acts at the cytoplasmic membrane...
January 6, 2018: Biochimica et Biophysica Acta
Nori Yoshioka, Matsuo Deguchi, Hideharu Hagiya, Hisao Yoshida, Norihisa Yamamoto, Shoji Hashimoto, Yukihiro Akeda, Kazunori Tomono
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) infections continue to be a leading problem in health care facilities worldwide. METHODS: This single-center retrospective cohort study consisted of a derivation phase and a validation phase. The derivation phase included all patients admitted to Osaka University Hospital between May 2010 and April 2011. We proposed a provisional available, bed-sided, comprehensive (ABC) score, and evaluated its accuracy using the clinical diagnosis as a reference...
January 8, 2018: BMC Infectious Diseases
Sooyeon Park, Jeehyun Choi, Su Jung Choi, Jung-Hoon Yoon
A Gram-stain-negative, facultatively aerobic, motile-by-gliding, non-flagellated and rod-shaped bacterial strain, designated YSM-43T, was isolated from a tidal flat in Yeosu on the South Sea in the Republic of Korea. Strain YSM-43T grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain YSM-43T fell within the clade comprising type strains of Flavobacterium species, clustering with the type strains of Flavobacterium jejuense and Flavobacterium jumunjinense...
January 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Ji-Hye Bu, Chang-Jun Cha
A Gram-staining-negative bacterial strain, designated CJ42T, was isolated from ginseng soil in Anseong, Republic of Korea. Cells were rod-shaped, yellow-pigmented, aerobic and devoid of flagella but showed gliding motility. Strain CJ42T grew optimally at 30 °C and pH 7.0 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain CJ42T belonged to the genus Flavobacterium within the family Flavobacteriaceae and was most closely related to Flavobacterium tistrianum KCTC 42679T (97...
January 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Dhiraj Kumar Chaudhary, Jaisoo Kim
A cream-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain Brt-FT, was isolated from oil-contaminated soil. Strain Brt-FT was able to grow from 15 to 45 °C, pH 6.5-10.5 and 0-4.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain Brt-FT represented a member of the genus Sphingobacterium and shared highest sequence similarity with Sphingobacterium chuzhouense DH-5T (99.4 %); Sphingobacterium gobiense H7T (95...
January 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Seung Seob Bae, Jaejoon Jung, Dawoon Chung, Kyunghwa Baek
A Gram-stain-negative, aerobic, motile, flagellated rod-shaped bacterium, designated ST58-10T, was isolated from an estuarine sediment in the Republic of Korea. The strain was able to degrade benzene. Growth of strain ST58-10T was observed at 4-35 °C (optimum, 20-25 °C), pH 5-9 (optimum, pH 7-8) and 1-8 % NaCl (optimum, 3 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ST58-10T formed a phyletic lineage within the genus Marinobacterium of the family Oceanospirillaceae...
January 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Olga I Nedashkovskaya, Song-Gun Kim, Larissa A Balabanova, Natalia V Zhukova, Irina Y Bakunina, Valery V Mikhailov
A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis. The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species. The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c...
January 5, 2018: International Journal of Systematic and Evolutionary Microbiology
Suhail Ahmed Almani, Ali Naseer, Sanjay Kumar Maheshwari, Pir Maroof, Raza Naseer, Haji Khan Khoharo
Objective: The present study aimed to evaluate the current trends of drug resistance patterns of Acinetobacter baumannii infection in blood transfusion-dependent thalassemia patients. Study Design: This study was a cross sectional study, conducted at the Liaquat University of Medical and Health Sciences, Jamshoro/Hyderabad, Sindh, Pakistan from October 2014 to January 2016. Subjects and Methods: Of 921 blood samples, A. baumannii strains were isolated from 100 blood samples...
October 2017: Journal of Global Infectious Diseases
Samuel Rosas, Daniel Rosas, Valentina Múnera Orozco, Manuela Parra Cardona, Chukwuweike Gwam, Simon Pedro Aristizabal
Pediatric septic arthritis can be a devastating disease. Often, the diagnosis can be challenging as autoimmune and infectious causes may present in a similar fashion. Thus, we present the case of a five-year-old male patient, from the Pacific coast of Colombia, with chronic morning knee pain and stiffness thought to be caused by an autoimmune disease. He presented with a mild effusion of the left knee, a flexed posture, and limited extension to 25°. Inflammatory markers demonstrated an infectious pattern. Autoimmune markers were negative...
December 22, 2017: Surgical Technology International
Jongguen Jeon, Leonid N Ten, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Young-Je Cho, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, motile by gliding, rod-shaped, aerobic bacterium, designated 15J6-3T6T, was isolated from a soil sample collected from Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J6-3T6T belongs to the family Cytophagaceae and is related to Larkinella harenae 15J9-9T (93.9 % similarity), Larkinella arboricola Z0532T (93.6 %), Larkinella bovis M2TB15T (93.3 %), and Larkinella insperata LMG 22510T (93...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
Longzhan Gan, Heming Zhang, Jiewei Tian, Xiaoguang Li, Xiufeng Long, Yuqin Zhang, Yumei Dai, Yongqiang Tian
A novel aerobic, Gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated LCB217T, was isolated from a saline-alkali soil in north-western China and identified using a polyphasic taxonomic approach. Growth occurred with 3-15 % (w/v) NaCl (optimum 3-5 %), at 10-45 °C (optimum 30 °C) and at pH 7.0-9.0 (optimum pH 9.0). Strain LCB217T contained MK-7 and MK-8 as the predominant menaquinones and anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0 as the major fatty acids...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
Masahiro Yuki, Mitsuo Sakamoto, Yuki Nishimura, Moriya Ohkuma
Strain Rs-Y01T was isolated from the gut of the wood-feeding subterranean termite Reticulitermes speratus. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain Rs-Y01T belonged to the genus Lactococcus and was most closely related to Lactococcus raffinolactis JCM 5706T with 98.1 % similarity in the 16S rRNA gene, followed by Lactococcus piscium JCM 16647T (97.2 %). Genomic comparisons of strain Rs-Y01T with L. raffinolactis JCM 5706Twere made using the Genome-to-Genome Distance Calculator and average nucleotide identity analysis (values indicated 29...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
Lili Niu, Mengjie Xiong, Juan Zhang, Yangquan Xiang, Lei Song, Ziyi Hua, Wenying Li
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %). The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
Myung Kyum Kim, Eun Tae Kim, Sang Bum Kim, Ha Yeon Jeong, Beom Young Park, Sathiyaraj Srinivasan
Gram-staining-positive, motile, rod-shaped bacteria, designated as H31022T and H31024 was isolated from rumen contents of a Holstein cow. Optimum growth occurred at 25°C and pH 7.0 on R2A agar medium. Oxidase and catalase activities are positive. The 16S rRNA gene sequence (1,452 bp) of the new isolates revealed they belong to the genus Kurthia of the phylum Firmicutes. Highest gene sequence similarities were assessed to be with Kurthia massiliensis JC30T (98.4%), Kurthia senegalensis JC8ET (97.5%), and Kurthia populi 10y-14T (97...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
Jae-Bong Lee, Sumin Hong, Seung-Yeol Lee, Su-Jin Park, Kyeung Il Park, Seok-Gwan Choi, Myung Kyum Kim, Leonid N Ten, Hee-Young Jung
The taxonomic position of bacterial strain, designated 15J16-1T3AT, recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence showed that strain 15J16-1T3AT belonged to the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to 'Larkinella harenae' 15J9-9 (95.9% similarity), Larkinella ripae 15J11-11T (95.6%), Larkinella bovis M2TB15T (94.7%), Larkinella arboricola Z0532T (93.9%), and Larkinella insperata LMG 22510T (93...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
Annarita Poli, Ida Romano, Vincenza Mastascusa, Lorena Buono, Pierangelo Orlando, Barbara Nicolaus, Luigi Leone, Kar Wai Hong, Kok-Gan Chan, Kian Mau Goh, Javier Pascual
Strain Corallo1T was isolated from mucus of red coral (Corallium rubrum) at Punta Pizzaco (Procida island, Naples, Italy). It was characterised as a Gram-stain negative, motile, rod-shaped bacterium. Strain Corallo1T was found to show positive responses for cytochrome-c oxidase, catalase, reduction of nitrate and nitrite, β-galactosidase activity and hydrolysis of starch, xylan, peptone, Tween 40, Tween 80 and casein. Strain Corallo1T was found to be mesophilic, neutrophilic to alkalophilic and slightly halophilic...
January 3, 2018: Antonie Van Leeuwenhoek
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