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Gram stain

Avinash Sharma, Kunal Jani, Guang-Da Feng, Prachi Karodi, Venkata Ramana Vemuluri, Hong-Hui Zhu, Sisinthy Shivaji, Vishal Thite, Swapnil Kajale, Praveen Rahi, Yogesh Shouche
A Gram-stain-negative, yellowish-orange pigmented, rod-shaped, motile bacterium, designated strain ARC111T , was isolated from sediment of Arctic permafrost at Midtre Lovénbreen glacier, Svalbard. 16S rRNA gene based identification of strain ARC111T demonstrated highest sequence similarities to Subsaxibacter broadyi P7T (97.8 %) and Subsaxibacter arcticus JCM30334T (97.5 %) and ≤95.2 % with all other members of the family Flavobacteriaceae. Phylogenetic analysis revealed the distinct positioning of strain ARC111T within the genus Subsaxibacter...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Hojun Sung, Hyun Sik Kim, June-Young Lee, Woorim Kang, Pil Soo Kim, Dong-Wook Hyun, Euon Jung Tak, Mi-Ja Jung, Ji-Hyun Yun, Min-Soo Kim, Na-Ri Shin, Tae Woong Whon, Jeong Rae Rho, Sun Duk Park, Hyung Eun Shim, Jin-Woo Bae
A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T , was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea). Strain AR23208T grew optimally at 25-30 °C, at pH 7 and in the absence of NaCl. Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T , respectively...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Qian Wang, Yali Song, Lina Choi, Hongyu Liu, Gejiao Wang, Mingshun Li
A Gram-stain-negative, non-motile, rod-shaped, red-pigmented strain, designated W37T , was isolated from soil near an iron factory in Busan (Republic of Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W37T was most closely related to Deinococcus yunweiensis YIM 007T (98.3 %) and Deinococcus radioresistens 8AT (96.3 %). The DNA-DNA relatedness between strain W37T and D. yunweiensis YIM 007T was 50.5 %. The predominant respiratory quinone was MK-8. The major polar lipids were an unidentified phosphoglycolipid, an unidentified aminophospholipid, four unidentified glycolipids, two unidentified phospholipids and an unidentified lipid...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Qing Liu, Yu-Hua Xin, Xiu-Ling Chen, Hong-Can Liu, Yu-Guang Zhou, Wen-Xin Chen
A Gram-stain-positive strain designated MDB1-42T was isolated from ice collected from Midui glacier in Tibet, PR China. Strain MDB1-42T was catalase-positive, oxidase-negative and grew optimally at 25-28 °C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that MDB1-42T represented a member of the genus Arthrobacter. The highest level of 16S rRNA gene sequence similarity (99.86 %) was found with Arthrobacter agilis NBRC 15319T . Multilocus sequence analysis revealed low similarity of 91...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Peter Kämpfer, Hans-Jürgen Busse, Stefanie P Glaeser
A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain KSS165-70T ) was isolated from a coolant lubricant emulsion. The 16S rRNA gene sequence analysis of strain KSS165-70T showed high sequence similarity to the type strains of Novosphingobium subterraneum (98.1 %), Novosphingobium lentum (97.9 %) and Novosphingobium taihuense (97.8 %). Sequence similarities to type strains of all other Novosphingobium species were below 97.5 %. Ubiquinone Q-10 was detected as the major respiratory quinone...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Can Chen, Nusratgul Anwar, Chen Wu, Geyi Fu, Ruijun Wang, Choangya Zhang, Yuehong Wu, Cong Sun, Min Wu
A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated MC28T , was isolated from storage liquid collected from the stems of Populus euphratica in the Xinjiang province of China. The growth range of NaCl concentration was 0.5-6.0 % (w/v), with an optimum at 3.0 % (w/v), the temperature range for growth was 10-45 °C, with an optimum at 40 °C, and the pH range for growth was 6.0-9.0, with an optimum around pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MC28T formed a distinct lineage in the clade of genus Halomonas and is closely related to Halomonas desiderata DSM 9502T (96...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Balaram Mohapatra, Pinaki Sar, Sufia Khannam Kazy, Mrinal Kumar Maiti, Tulasi Satyanarayana
Reductive transformation of toxic arsenic (As) species by As reducing bacteria (AsRB) is a key process in As-biogeochemical-cycling within the subsurface aquifer environment. In this study, we have characterized a Gram-stain-negative, non-spore-forming, rod-shaped As reducing bacterium designated KAs 5-3T, isolated from highly As-contaminated groundwater of India. Strain KAs 5-3T displayed high 16S rRNA gene sequence similarity to the members of the genus Pseudoxanthomonas, with P. mexicana AMX 26BT (99.25% similarity), P...
2018: PloS One
Brenda K Schroeder, William L Schneider, Douglas G Luster, Aaron Sechler, Timothy D Murray
Aplanobacter agropyri was first described in 1915 by O'Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species. In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA. In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011...
March 20, 2018: International Journal of Systematic and Evolutionary Microbiology
Yuli Wei, Jiasong Fang, Yunping Xu, Weiqiang Zhao, Junwei Cao
A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6T , was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 35 °C (range 22-41 °C), at pH 6 (4-8) and with 4 % (w/v) NaCl (0-10 %). The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 °C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6T belonged to the genus Corynebacterium, with the highest sequence similarity (97...
March 20, 2018: International Journal of Systematic and Evolutionary Microbiology
G López, C Díaz-Cárdenas, J David Alzate, L N Gonzalez, N Shapiro, T Woyke, N C Kyrpides, S Restrepo, S Baena
Three morphologically similar thermo-acidophilic strains, USBA-GBX-501, USBA-GBX-502 and USBA-GBX-503T , were isolated from acidic thermal springs at the National Natural Park Los Nevados (Colombia). All isolates were spore-forming, Gram-stain-positive and motile, growing aerobically at 25-55 °C (optimum ~45 °C) and at pH 1.5-4.5 (optimum pH ~3.0). Phylogenetic analysis of the 16S rRNA gene sequences of these isolates showed an almost identical sequence (99.0 % similarity) and they formed a robust cluster with the closest relative Alicyclobacillus tolerans DSM 16297T with a sequence similarity of 99...
March 20, 2018: International Journal of Systematic and Evolutionary Microbiology
Tang Gao, Hongliang Zeng, Huan Xu, Feng Gao, Wei Li, Shengwang Zhang, Yi Liu, Guifang Luo, Mingdan Li, Dejian Jiang, Zhigao Chen, Yong Wu, Wei Wang, Wenbin Zeng
Background: Increasing bacterial infections as well as a rise in bacterial resistance call for the development of novel and safe antimicrobial agents without inducing bacterial resistance. Nanoparticles (NPs) present some advantages in treating bacterial infections and provide an alternative strategy to discover new antibiotics. Here, we report the development of novel self-assembled fluorescent organic nanoparticles ( FONs ) with excellent antibacterial efficacy and good biocompatibility. Methods: Self-assembly of 1-(12-(pyridin-1-ium-1-yl)dodecyl)-4-(1,4,5-triphenyl-1H-imidazol-2-yl)pyridin-1-ium (TPIP) in aqueous solution was investigated using dynamic light scattering (DLS) and transmission electron microscopy (TEM)...
2018: Theranostics
Pei Lin, Zheng-Fei Yan, Chang-Tian Li, MooChang Kook, Tae-Hoo Yi
A Gram-stain negative, aerobic, motile by flagella, rod-shaped strain (THG-T16T ) was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6.0-8.0 (optimum 7.0) and at 0-1.0% NaCl (optimum 0%). Based on 16S rRNA gene sequence analysis, the near phylogenetic neighbours of strain THG-T16T were identified as Nibribacter koreensis KACC 16450T (98.6%), Rufibacter roseus KCTC 42217T (94.7%), Rufibacter immobilis CCTCC AB 2013351T (94.5%) and Rufibacter tibetensis CCTCC AB 208084T (94...
March 16, 2018: Antonie Van Leeuwenhoek
Taisa Trevizani Rocchetti, Katheryne Benine Martins, Patricia Yoshida Faccioli Martins, Rogério Antonio de Oliveira, Alessandro Lia Mondelli, Carlos Magno Castelo Branco Fortaleza, Maria de Lourdes Ribeiro de Souza da Cunha
INTRODUCTION: Staphylococcus spp. - both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) - are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality. MATERIAL AND METHODS: In this study we evaluated a multiplex PCR standardized to detect Staphylococcus spp...
March 13, 2018: Brazilian Journal of Infectious Diseases
Tsuneaki Kenzaka, Ayako Kumabe, Koki Kosami, Yasufumi Matsuoka, Kensuke Minami, Daisuke Ninomiya, Ayako Noda, Shinsuke Yahata
BACKGROUND: Clinical practice guidelines for nursing- and healthcare-associated pneumonia (NHCAP) were developed for pneumonia caused by drug-resistant bacteria and pneumonia in elderly patients, particularly aspiration pneumonia. The identification of pathogenic bacteria and implementation of efforts to prevent the recurrence of aspiration pneumonia are very important in clinical practice. This study examined the extent to which clinicians have established bacteriological testing and recurrence prevention efforts for NHCAP and aspiration pneumonia...
March 2018: Respiratory Investigation
Jian-Chao Zhao, Juan Cheng, Qi Zhang, Zi-Wei Gao, Meng-Yue Zhang, Yi-Xuan Zhang
A Gram-stain-negative, strictly aerobic, yellow-coloured, motile by gliding and elongated rod-shaped bacterial strain, designated SYP-B1015T , was isolated from the rhizosphere of Artemisia annua L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-B1015T belonged to the genus Flavobacterium and had highest 16S rRNA gene sequence similarity to Flavobacterium compostarboris JCM 16527T (98.1 %) and Flavobacterium procerum JCM 30113T (97.2 %). The predominant respiratory quinone for the strain was MK-6, and the major cellular fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH...
March 16, 2018: International Journal of Systematic and Evolutionary Microbiology
Rosa Margesin, De-Chao Zhang, Luciana Albuquerque, Hugo J C Froufe, Conceição Egas, Milton S da Costa
A Gram-stain-negative, rod-shaped, motile, catalase-positive and cytochrome c oxidase-positive bacterial strain, designated AM20-91T , was isolated from alpine forest soil. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain AM20-91T was related to the genus Lysobacter and had highest 16S rRNA gene sequence similarities to the type strains of Lysobacter novalis THG-PC7T (97.8 %), Luteimonas tolerans UM1T (97.7 %) and Lysobacter ximonensis XM415T (97.0 %). The strain contained ubiquinone 8 as the predominant respiratory quinone; its polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminophospholipids...
March 16, 2018: International Journal of Systematic and Evolutionary Microbiology
Makoto Chiba, Takanori Itabashi, Koujiro Hirai, Mitsuo Sakamoto, Moriya Ohkuma, Taichiro Ishige, Shinji Kawasaki
A strain (Hime 5-1T ) of lactic acid bacterium was isolated from the gut of the grasshopper Metrioptera engelhardti from a mountainous area of Nagano Prefecture, Japan. Strain Hime 5-1T had a low 16S rRNA gene sequence similarity to known lactic acid bacteria, with the closest recognized relatives being Lactobacillus tucceti (96.7 %), Lactobacillus furfuricola (96.5 %), Lactobacillus versmoldensis (96.3 %) and Lactobacillus nodensis (96.1 %). Comparative analyses of the rpoA and pheS gene sequences indicated that Hime 5-1T is not closely related to other Lactobacillus species...
March 15, 2018: International Journal of Systematic and Evolutionary Microbiology
Bin Chen, Wenqi Zhang, Bin Ji, Qingjie Ma, Dandan Li, Shi Gao
The present study aimed to determine the utility of99m Tc-3PRGD2 single photon emission computed tomography (SPECT)/computed tomography (CT) for the non-invasive monitoring of the response of integrin αv β3 expression to anti-angiogenic treatment with bevacizumab. Bevacizumab or vehicle therapy was performed in athymic nu/nu mice bearing A549 lung tumors (moderately high integrin αv β3 expression) or PC-3 prostate tumors (low integrin αv β3 expression) at a dose of 1 mg twice a week. The average tumor volume was 180±90 mm3 the day prior to baseline SPECT/CT...
April 2018: Oncology Letters
Min-Jiao Liu, Chun-Zhi Jin, Mipeshwaree Devi Asem, Yoon-Jung Ju, Dong-Jin Park, Nimaichand Salam, Min Xiao, Wen-Jun Li, Chang-Jin Kim
A Gram-stain negative, strictly aerobic and non-motile bacterium, designated strain BN130233T , was isolated from a soil sample collected from Gyeongsangbuk-do, Republic of Korea. Colonies were orange in colour, with wet and smooth surfaces. Phylogenetic analyses based on the 16S rRNA gene sequences resulted in strain BN130233T forming a cluster with members of the family ChitinophagaceaeKämpfer et al. 2011, while sharing the highest sequence identity of 91.2 % with Chitinophaga niastensis JS16-4T . Good growth was observed at 20-28 °C, pH 7...
March 14, 2018: International Journal of Systematic and Evolutionary Microbiology
Ram Hari Dahal, Jaisoo Kim
A novel α-proteobacterium, designated strain S-54T , was isolated from forest soil sampled at Kyonggi University and subjected to polyphasic study. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Strain S-54T assimilated lactic acid, d-glucose and 4-hydroxybenzoic acid. Strain S-54T tolerated 4 % NaCl (w/v), and grew optimally at 45 °C and pH 10.5. Phylogenetic analysis based on 16S rRNA gene sequence data revealed that strain S-54T formed a lineage within the class Alphaproteobacteria of the phylum Proteobacteria that was distinct from various members of the genus Altererythrobacter, including Altererythrobacter troitsensis JCM 17037T (96...
March 14, 2018: International Journal of Systematic and Evolutionary Microbiology
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