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https://www.readbyqxmd.com/read/28102738/optimization-and-partial-characterization-of-intracellular-anticandidal-protein-from-aspergillus-giganteus-mtcc-8408-using-taguchi-doe
#1
Debashis Dutta, Mira Debnath Das
A new intracellular antifungal protein (afp) production with average molecular weight 24.3 kDa and yield of 0.65 ± 0.1 mg/gram dry cell weight (gdcw) of mycelia in submerged fermentation of Aspergillus giganteus MTCC 8408 was optimized. Taguchi's DOE (design of experiment) L27 orthogonal array (OA) was constructed using Qualitek-4 software with 8 most influensive factors namely, culture pH, temperature, slant age, inoculum volume, agitation and KH2PO4. Scanning electron microscopy (SEM) was used to correlate the effect of selected factors on fungal cell morphology and afp production...
January 19, 2017: Bioengineered
https://www.readbyqxmd.com/read/28101272/isolation-and-characterization-of-agrobacterium-strains-from-soil-a-laboratory-capstone-experience
#2
Kim R Finer, Lee Fox, John J Finer
In this investigation, the students' goal was to isolate and characterize Agrobacterium strains from soil. Following selection and enrichment on 1A-t medium, putative Agrobacterium isolates were characterized by Gram stain reaction and biochemical tests. Isolates were further evaluated using polymerase chain reaction (PCR) with different primer sets designed to amplify specific regions of bacterial deoxyribonucleic acid (DNA). Primer sets included AGRH to identify isolates that were members of the Rhizobiaceae, BIOVAR1 primers to identify members of Agrobacterium biovar group I, and a third set, VIRG, to determine presence of virG (only present in pathogenic Agrobacterium strains)...
December 2016: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28100309/nioella-sediminis-sp-nov-isolated-from-the-surface-sediment-and-emended-description-of-the-genus-nioella
#3
Yang Liu, Juan Du, Qiliang Lai, Chunming Dong, Zongze Shao
A bacterial strain, designated JS7-11T, was isolated from the surface sediment of the Jiulong River and characterized using a polyphasic taxonomic approach. Cells of strain JS7-11T was Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 10-40°C (optimum 25-30°C), at pH 6-8 (optimum 7) and in 1-12% NaCl (optimum 3-4%, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JS7-11T and type strain Nioella nitratireducens SSW136T formed an independent cluster, sharing 97...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28100298/description-of-two-novel-members-of-the-family-erysipelotrichaceae-ileibacterium-valens-gen-nov-sp-nov-and-dubosiella-newyorkensis-gen-nov-sp-nov-from-the-murine-intestine-and-emendation-to-the-description-of-faecalibacterium-rodentium
#4
Laura M Cox, Jiho Sohn, Kerin L Tyrrell, Diane M Citron, Paul A Lawson, Nisha B Patel, Tadasu Iizumi, Guillermo I Perez-Perez, Ellie J C Goldstein, Martin J Blaser
To better characterize murine intestinal microbiota, a large number (187) of Gram-positive staining, rod- and coccoid-shaped facultative and strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene similarity values of 87...
January 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28100271/evaluation-of-three-sample-preparation-methods-for-the-direct-identification-of-bacteria-in-positive-blood-cultures-by-maldi-tof
#5
Hannah Tanner, Jason T Evans, Savita Gossain, Abid Hussain
BACKGROUND: Patient mortality is significantly reduced by rapid identification of bacteria from sterile sites. MALDI-TOF can identify bacteria directly from positive blood cultures and multiple sample preparation methods are available. We evaluated three sample preparation methods and two MALDI-TOF score cut-off values. Positive blood culture bottles with organisms present in Gram stains were prospectively analysed by MALDI-TOF. Three lysis reagents (Saponin, SDS, and SepsiTyper lysis bufer) were applied to each positive culture followed by centrifugation, washing and protein extraction steps...
January 18, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28073405/rhodanobacter-humi-sp-nov-a-novel-acidtolerant-and-alkalitolerant-gammaproteobacterium-isolated-from-kyonggi-university-forest-soil
#6
Ram Hari Dahal, Jaisoo Kim
A novel acidtolerant and alkalitolerant gammaproteobacterium designated strain RS22T was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-staining-negative, catalase- and oxidase- positive, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. Flexirubin-type pigments were absent. Strain RS22T assimilates lactic acid, L-proline, and 3-hydroxybenzoic acid; tolerates 4 % of NaCl, ferments glucose, and can grow at pH 11.0. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RS22T formed a lineage within the class Gammaproteobacteria of the phylum Proteobacteria that was distinct from various species of the genus Rhodanobacter, including Rhodanobacter denitrificans 2APBS1T (97...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28073403/arcanobacterium-wilhelmae-sp-nov-isolated-from-the-genital-tract-of-a-rhinoceros-rhinoceros-unicornis
#7
Osama Sammra, Jörg Rau, Jörn-Peter Wickhorst, Mazen Alssahen, Abdulwahed Ahmed Hassan, Christoph Lämmler, Peter Kämpfer, Stefanie P Glaeser, Hans-Jürgen Busse, Tanita Kleinhagauer, Tobias Knauf-Witzens, Ellen Prenger-Berninghoff, Amir Abdulmawjood, Günter Klein
AbstractA polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8 %), Arcanobacterium phocisimile (97.8 %), Arcanobacterium phocae (97.7%), Arcanobacterium haemolyticum (97.4 %), Arcanobacterium hippocoleae (96.6 %), Arcanobacterium pinnipediorum (96...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28069785/postoperative-mycoplasma-hominis-brain-abscess-keep-it-in-mind
#8
Sarah Maria Bergin, Shehara M Mendis, Barnaby Young, Ezlyn Binti Izharuddin
A temporal lobe abscess was diagnosed in a 57-year-old man. A urethral catheter had been inserted 12 days earlier, just prior to clot evacuation of a subacute haematoma secondary to an arterio-venous malformation. Fever persisted despite debridement and treatment with meropenem and vancomycin. Gram stains of operative samples showed no bacteria. Extended cultures grew pinpoint colonies after 5 days. Meanwhile, sequencing of bacterial 16S rDNA from operative specimens had identified Mycoplasma hominis; the bacterial colonies were subsequently similarly identified...
January 9, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28068219/hyphobacterium-vulgare-gen-nov-sp-nov-a-novel-alpha-proteobacterium-isolated-from-seawater-in-the-south-china-sea
#9
Cong Sun, Rui-Jun Wang, Yue Su, Ge-Yi Fu, Zhe Zhao, Xiao-Yun Yu, Chong-Ya Zhang, Can Chen, Shuai-Bo Han, Meng-Meng Huang, Zheng-Bing Lv, Min Wu
A Gram-staining-negative, aerobic, non-spore-forming, non-motile, oval to rod shaped bacteria with prosthecate, designated as WM6T, was isolated from seawater sample collected from the South China Sea at depth of 150 meter and subjected to a polyphasic taxonomic investigation. Cells of strain WM6T were approximately 0.5-0.6 μm in width and 0.8-1.2 μm in length, and colonies of strain WM6T were smooth, circular, convex and whity-yellow. Strain WM6T was found to grow at 10-45 °C (optimum, 30 °C), pH 6.5-9...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28068215/tibeticola-sediminis-gen-nov-sp-nov-a-thermophilic-bacterium-isolated-from-hot-spring
#10
Inam Ullah Khan, Firasat Hussain, Ye Tian, Neeli Habib, Wen-Dong Xian, Zhao Jiang, Arshia Amin, Chang-Guo Yuan, En-Min Zhou, Xiao-Yang Zhi, Wen-Jun Li
Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008 were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. Taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-staining negative, aerobic, short rod shaped and motile by means of a polar flagellum. They were oxidase and catalase positive and were able to grow at 30-55 °C (optimum, 37-45 °C), pH 6.0-8.0 (optimum, pH 7...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28068214/paenibacillus-rhizoplanae-sp-nov-isolated-from-the-rhizosphere-of-zea-mays
#11
Peter Kämpfer, Hans-Jürgen Busse, John A McInroy, Chia-Hui Hu, Joseph W Kloepper, Stefanie P Glaeser
A Gram-positive staining, aerobic, endospore-forming bacterial strain, isolated from the rhizosphere of Zea mays was studied for its detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-64T was shown to be a member of the genus Paenibacillus, most closely related to the type strains of Paenibacillus silagei (99%) and Paenibacillus borealis (97.5%). The 16S rRNA gene sequence similarity to all other Paenibacillus species was ≤ 97.5%. DNA-DNA hybridization values to the type strains of P...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28061821/unusual-manifestation-of-helicobacter-cinaedi-infection-a-case-report-of-intracranial-subdural-empyema-and-bacteremia
#12
Toshimasa Hayashi, Junko Tomida, Yoshiaki Kawamura, Masakazu Yoshida, Ikuyo Yokozawa, Shingaku Kaneko
BACKGROUND: There have been various reports concerning Helicobacter cinaedi infections. However, few reports have examined central nervous system infections. CASE PRESENTATION: A 52-year-old man was transferred from the local hospital because of a persistent headache and suspected intracranial subdural empyema. Neurosurgical drainage was performed via burr holes. Gram staining and results from abscess cultures were negative. The blood culture yielded H. cinaedi...
January 7, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28056226/flectobacillus-pallidus-sp-nov-isolated-from-a-spring
#13
Shih-Yi Sheu, Li-Pin Liu, Wen-Ming Chen
A novel bacterial strain MVW-6T was isolated from a spring in Taiwan and characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-6T belonged to the genus Flectobacillus and showed the highest levels of sequence similarity with respect to Flectobacillus lacus CL-GP79T (98.2 %) and Flectobacillus fontis MIB-4T (97.4 %). Cells of strain MVW-6T were Gram-staining-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-colored colonies...
January 5, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28056223/arthrobacter-halophytocola-sp-nov-a-novel-endophytic-actinomycete-isolated-from-the-roots-of-a-coastal-halophyte-limonium-sinense
#14
Wei-Wei Feng, Tian-Tian Wang, Juan-Luan Bai, Peng Ding, Ke Xing, Ji-Hong Jiang, Xue Peng, Sheng Qin
A novel actinobacterium designated KLBMP 5180T, was isolated from the surface-sterilized root of a coastal halophyte Limonium sinense collected from the city of Lianyungang, Jiangsu Province, east of China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9 (70%). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and one unknown phospholipid...
January 5, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28056220/aliidiomarina-sedimenti-sp-nov-a-haloalkaliphilic-bacterium-in-the-family-idiomarinaceae
#15
Azadeh Shahinpei, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Cathrin Spröer, Antonio Ventosa
A novel Gram-staining-negative straight or curved rod, moderately halophilic and alkaliphilic bacterium, designated strain GBSy1T, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Strain GBSy1T was motile, and formed non-pigmented, mucoid colonies. Growth occurred between 1 and 15 % (w/v) NaCl and the isolate grew optimally with 5 % (w/v) NaCl. The optimum pH and temperature for growth were 8.5 and 34 °C, while the strain was able to grow at pH 7.0-10 and 4-40 °C. On the basis of 16S rRNA gene sequence analysis, strain GBSy1T was shown to belong to the genus Aliidiomarina within the class Gammaproteobacteria, family Idiomarinaceae and showed closest phylogenetic similarity to Aliidiomarina maris CF12-14T (97...
January 4, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28053527/fabrication-of-nonfouling-bactericidal-and-bacteria-corpse-release-multifunctional-surface-through-surface-initiated-raft-polymerization
#16
Bailiang Wang, Zi Ye, Yihong Tang, Yuemei Han, Quankui Lin, Huihua Liu, Hao Chen, Kaihui Nan
Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA-co-MPC)) brush was synthesized by "grafting from" method through reversible-addition fragmentation chain transfer polymerization...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28052587/pilot-study-of-dornase-alfa-pulmozyme-therapy-for-acquired-ventilator-associated-infection-in-preterm-infants
#17
Melissa Scala, Deborah Hoy, Maria Bautista, Judith Jones Palafoutas, Kabir Abubakar
OBJECTIVE: Evaluate the feasibility, safety, and efficacy of adjunctive treatment with dornase alfa in preterm patients with ventilator-associated pulmonary infection (VAPI) compared to standard care. WORKING HYPOTHESIS: We hypothesize that therapy with dornase alfa will be safe and well tolerated in the preterm population with no worsening of symptoms, oxygen requirement, or need for respiratory support. STUDY DESIGN: Prospective, randomized, blinded, pilot study comparing adjunctive treatment with dornase alfa to sham therapy...
January 3, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28051856/enhanced-cellular-internalization-a-bactericidal-mechanism-more-relative-to-biogenic-nanoparticles-than-chemical-counterparts
#18
Madhuree Kumari, Shatrunajay Shukla, Shipra Pandey, Ved P Giri, Anil Bhatia, Tusha Tripathi, Poonam Kakkar, Chandra Shekhar Nautiyal, Aradhana Mishra
Biogenic synthesis of silver nanoparticles for enhanced antimicrobial activity has gained a lot of momentum making it an urgent need to search for a suitable bio-candidate which could be utilized for efficient capping and shaping of silver nanoparticles with enhanced bactericidal activity utilising its secondary metabolites. Current work illustrates the enhancement of antimicrobial efficacy of silver nanoparticles by reducing and modifying their surface with antimicrobial metabolites of cell free extract of Trichoderma viride (MTCC 5661) in comparison to citrate stabilized silver nanoparticles...
January 4, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28050502/antibacterial-effect-of-aloe-vera-gel-against-oral-pathogens-an-in-vitro-study
#19
Supreet Jain, Nirav Rathod, Ravleen Nagi, Jaideep Sur, Afshan Laheji, Naveen Gupta, Priyanka Agrawal, Swati Prasad
INTRODUCTION: Natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. Aloe Vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties. AIM: The aim of the present study was to determine the anti-microbial and inhibitory activities of various concentration of Aloe Vera Gel (AVG) against oral pathogenic bacteria...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28036317/complicated-skin-and-soft-tissue-infection-with-mycobacterium-fortuitum-following-excision-of-a-sebaceous-cyst-in-taiwan
#20
Shih-Chen Tsai, Li-Hsin Chen, Hsien-Hua Liao, Chih-Yu Chiang, Wea-Lung Lin, Shiuan-Chih Chen, Shih-Ming Tsao, Hung-Chang Hung, Yuan-Ti Lee
Mycobacterium fortuitum group (M. fortuitum), also known as rapidly growing Mycobacteria, can cause pyogenic infections in human beings, most commonly in immunocompromised patients. Herein, we present a 40-year-old immunocompetent male patient who underwent planned excision of a sebaceous cyst in the abdominal wall. He suffered from tender erythematous lesions with purulent discharge around the healing wound that developed 2 weeks after surgery. Gram stain, bacterial and fungal culture results of the wound were negative...
December 30, 2016: Journal of Infection in Developing Countries
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