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https://www.readbyqxmd.com/read/28721940/isolation-characterisation-and-enzymatic-activity-of-streptomyces-sp-and-its-ph-control-during-fermentation-process
#1
Balraj Baskaran, Arulmozhi Muthukumarasamy
Streptomyces sp. with grey white mycelium was isolated from Kodiyakkarai marine segment of east coastal of India. The isolate produced spiny spores and did not produce any diffusible pigments. Various biochemical tests were carried out to confirm the isolate classification. The amylolytic enzyme activity was estimated using the isolated strain which was confirmed by golden yellowish zone formation after using the Gram's iodine stain. The amount of amylase produced by the isolate was 6.15 U/ml. Higher living cells always produce better enzymes, so that the process parameters especially pH was maintained in fermentation chamber for higher growth and also for large-scale growth...
August 2017: IET Systems Biology
https://www.readbyqxmd.com/read/28721854/flavitalea-antarctica-sp-nov-isolated-from-fildes-peninsula-antarctica
#2
Ziyan Wei, Yao Huang, Wangmu Danzeng, Myong Chol Kim, Guoxin Zhu, Yumin Zhang, Zuobing Liu, Fang Peng
A bright-yellow, Gram-stain-negative, rod-shaped, gliding and aerobic bacterium, designated strain AQ6-291T, was isolated from the Fildes Peninsula, Antarctica, and its taxonomic position was investigated by genotypic, phenotypic and chemotaxonomic analyses. Growth occurred at 4-28 °C (optimum 20 °C) and at pH 5.0-8.0 (optimum pH 7.0). Strain AQ6-291T contained iso-C15 : 1 G, iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) as the major cellular fatty acids...
July 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28721843/treponema-rectale-sp-nov-a-spirochete-isolated-from-the-bovine-rectum
#3
Gareth J Staton, Kerry Newbrook, Simon R Clegg, Richard J Birtles, Nicholas J Evans, Stuart D Carter
A Gram-stain-negative, obligatory anaerobic spirochete, CHPAT, was isolated from the rectal tissue of a Holstein-Friesian cow. On the basis of 16S rRNA gene comparisons, CHPAT was most closely related to the human oral spirochete, Treponema parvum, with 88.8 % sequence identity. Further characterisation on the basis of recA gene sequence analysis, cell morphology, pattern of growth and physiological profiling identified marked differences with respect to other recognised species of the genus Treponema. Microscopically, the helical cells measured approximately 1-5 µm long and 0...
July 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28719657/isolation-and-in-vitro-evaluation-of-bacteriophages-against-mdr-bacterial-isolates-from-septic-wound-infections
#4
Roja Rani Pallavali, Vijaya Lakshmi Degati, Dakshayani Lomada, Madhava C Reddy, Vijaya Raghava Prasad Durbaka
Multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. The use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. Our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. Pus samples were aseptically collected from Rajiv Gandhi Institute of Medical Science (RIMS), Kadapa, A...
2017: PloS One
https://www.readbyqxmd.com/read/28719354/thymol-and-carvacrol-strongly-inhibit-biofilm-formation-and-growth-of-carbapenemase-producing-gram-negative-bacilli
#5
P Raei, T Pourlak, M Y Memar, N Alizadeh, M Aghamali, E Zeinalzadeh, M Asgharzadeh, H S Kafil
Discovery of novel drugs with new mechanisms of action and without cross-reaction with current therapeutic agents is crucial in the management of infections caused by multi-drug resistant (MDR) bacteria. The aim of the present study was to investigate effects of carvacrol and thymol on biofilm formation and antimicrobial activity against different carbapenemase-producing Gram negative bacilli. The antimicrobial and antibiofilm effect of thymol and carvacrol was investigated against strains harboring different genes related to carbapenemase resistance...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28719324/susceptibility-of-clinical-isolates-of-burkholderia-pseudomallei-to-a-lipid-a-biosynthesis-inhibitor
#6
Sineenart Sengyee, Natnaree Saiprom, Suporn Paksanont, Direk Limmathurotsakul, Vanaporn Wuthiekanun, Narisara Chantratita
Burkholderia pseudomallei is the causative agent of melioidosis, a serious infection associated with high mortality and relapse. Current antimicrobial therapy using ceftazidime (CAZ) is often ineffective. Inhibitors of LpxC, the enzyme responsible for lipid A biosynthesis, have potential antimicrobial activity against several Gram-negative bacteria in vivo, but their activity against B. pseudomallei is unclear. Herein, we investigated the susceptibility of B. pseudomallei clinical isolates to LpxC-4, an LpxC inhibitor, and LpxC-4 in combination with CAZ...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28714845/bacillus-mangrovi-sp-nov-isolated-from-a-sediment-sample-from-a-mangrove-forest
#7
Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28714844/puzihella-rosea-gen-nov-sp-nov-a-novel-member-of-the-family-microbacteriaceae-isolated-from-freshwater
#8
Shih-Yi Sheu, Li-Ping Liu, Wen-Ming Chen
Two Gram-stain-positive, aerobic, pink, curved, rod-shaped, non-motile bacterial strains, designated MI-28T and SKY-11, were isolated from freshwater samples taken from a river and fish pond, respectively. Based on characterization using a polyphasic approach, the two strains showed highly similar phenotypic, physiological and genetic profiles. They demonstrated 99.9 % 16S rRNA gene sequence similarity and a 93-95 % DNA-DNA relatedness value, suggesting that they represent a single genomic species. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strains MI-28T and SKY-11 form a distinct lineage with respect to closely related genera within the family Microbacteriaceae of the class Actinobacteria, which is most closely related to Rhodoluna and Pontimonas, and levels of 16S rRNA gene sequence similarity with the type species of related genera were less than 95 %...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28714843/roseomonas-suffusca-sp-nov-isolated-from-lagoon-sediments
#9
Y Subhash, Sang-Seob Lee
Three light-brown-coloured, Gram-stain-negative, small rod- to oval-shaped, motile bacteria were isolated from lagoon sediments collected from North Carolina, USA. The strains (S1T, AS3 and AS6) grew aerobically at 13-42 °C (optimum, 30-35 °C) and pH 6.5-9.1 (optimum, pH 7.0-7.5). All three strains were positive for catalase and oxidase activity, and no added NaCl was required for growth. C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c, C18 : 1ω7c 11-methyl and C16 : 0 were the predominant fatty acids (>5 %)...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28708058/kiloniella-antarctica-sp-nov-isolated-from-a-polynya-of-amundsen-sea-in-western-antarctic-sea
#10
Ok-Ja Si, Hye-Young Yang, Chung Yeon Hwang, So-Jeong Kim, Sun-Bin Choi, Jong-Geol Kim, Man-Young Jung, Song-Gun Kim, Seong Woon Roh, Sung-Keun Rhee
A taxonomic study was conducted on strain soj2014T, which was isolated from the surface water of a polynya in the Antarctic Sea. Comparative 16S rRNA gene sequence analysis showed that strain soj2014T belongs to the family Kiloniellaceae and is closely related to Kiloniella spongiae MEBiC09566T, 'Kiloniella litopenaei' P1-1T and Kiloniella laminariae LD81T (98.0 %, 97.8 % and 96.2 % 16S rRNA gene sequence similarity, respectively). The DNA-DNA hybridization values between strain soj2014T and closely related strains were below 28...
July 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28706386/alarming-emergence-molecular-characterization-and-outcome-of-blandm-1-in-patients-infected-with-multidrug-resistant-gram-negative-bacilli-in-a-tertiary-care-hospital
#11
Huma Naim, Meher Rizvi, Mohd Azam, Richa Gupta, Neelam Taneja, Indu Shukla, Haris M Khan
INTRODUCTION: This study was conducted to assess the prevalence of metallo-beta-lactamases (MBLs) in general and blaNDM-1 in particular. It also aimed at evaluating clinical characteristics and outcome in patients infected with MBLs. MATERIALS AND METHODS: A total of 116 carbapenem-resistant Gram-negative bacilli (CRGNB) were evaluated in the study. These CRGNB were tested for MBL production both phenotypically for MBLs and genotypically for blaNDM-1 gene by polymerase chain reaction (PCR)...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28702079/rare-bacterial-isolates-causing-bloodstream-infections-in-ethiopian-patients-with-cancer
#12
Balew Arega, Yimtubezinash Wolde-Amanuel, Kelemework Adane, Ezra Belay, Abdulaziz Abubeker, Daniel Asrat
BACKGROUND: In recent years, saprophytic bacteria have been emerging as potential human pathogens causing life-threatening infections in patients with malignancies. However, evidence is lacking concerning such bacteria, particularly in sub-Saharan countries. This study was designed to determine the spectrum and drug resistance profile of the rare bacterial pathogens causing bloodstream infections (BSIs) in febrile cancer patients at a referral hospital in Ethiopia. METHODS: Between December 2011 and June 2012, blood samples were collected from 107 patients with cancer in Tikur Anbessa hospital...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28699882/thalassotalea-litorea-sp-nov-isolated-from-seashore-sand
#13
Heeyoung Kang, Haneul Kim, In-Young Nam, Yochan Joung, Tae Yong Jang, Kiseong Joh
A novel Gram-stain-negative, aerobic and rod-shaped marine bacterium, designated strain HMF4135T, was isolated from a sand sample which was collected from the seashore of the South Sea, Republic of Korea. It required NaCl for growth and exhibited optimal growth at 30 °C, with 2 % (w/v) NaCl and at pH 7-8. Cellular fatty acids were dominated by C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω9c and C12 : 0 3-OH. The predominant isoprenoid quinone was ubiquinone-8 (Q-8)...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699880/paenibacillus-konkukensis-sp-nov-isolated-from-animal-feed
#14
Wan-Taek Im, Kwon-Jung Yi, Sang-Suk Lee, Hyung In Moon, Che Ok Jeon, Dong-Woon Kim, Soo-Ki Kim
A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699877/isolation-and-characterization-of-kingella-negevensis-sp-nov-a-novel-kingella-species-detected-in-a-healthy-paediatric-population
#15
Nawal El Houmami, Sofiane Bakour, Janek Bzdrenga, Jaishiram Rathored, Hervé Seligmann, Catherine Robert, Nicholas Armstrong, Jacques Schrenzel, Didier Raoult, Pablo Yagupsky, Pierre-Edouard Fournier
We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699874/algoriphagus-marinus-sp-nov-isolated-from-marine-sediment-and-emended-description-of-the-genus-algoriphagus
#16
Ji-Ru Han, Qing-Li Geng, Feng-Qing Wang, Zong-Jun Du, Guan-Jun Chen
A Gram-stain-negative, facultatively anaerobic coccobacilli approximately 0.4-0.5×0.5-1.1 µm, non-motile, orange-pigmented bacterial strain, designated am2T, was isolated from the coastal area of Weihai, PR China (121° 57' E, 37° 29' N). The temperature, pH and NaCl ranges for growth were 4-40 °C, pH 6.5-8.5 and 0.0-6.0 % (w/v) NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences revealed that am2T was phylogenetically related to members of the genus Algoriphagus and was closely related to Algoriphagus boritolerans, Algoriphagus namhaensis and Algoriphagus antarcticus with 96...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699868/flaviramulus-aestuariivivens-sp-nov-isolated-from-a-tidal-flat
#17
Sooyeon Park, Min-Ji Ha, Sun Young Yoon, Jung-Hoon Yoon
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and ovoid or rod-shaped bacterium, designated OITF-51T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-51T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-51T forms a cluster with the type strains of three species of the genus Flaviramulus...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699867/sporolactobacillus-shoreicorticis-sp-nov-a-lactic-acid-producing-bacterium-isolated-from-tree-bark
#18
Vasana Tolieng, Budsabathip Prasirtsak, Mika Miyashita, Chiyo Shibata, Naoto Tanaka, Nuttha Thongchul, Somboon Tanasupawat
A Gram-stain-positive, lactic acid-producing bacterium designed strain MK21-7T, was isolated from tree bark collected from the north east of Thailand. This strain was a facultatively anaerobic spore-forming rod that was catalase-negative. It contained meso-diaminopimelic acid in the cell wall peptidoglycan and had seven isoprene units (MK-7) as the predominant menaquinone. Major fatty acids of MK21-7T were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and C18 : 1ω9c. Polar lipids were phosphatidglycerol, diphosphatidylglycerol, an unknown phospholipid, three unknown glycolipids and an unknown lipid...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699866/description-of-lysinibacillus-telephonicus-sp-nov-isolated-from-the-screen-of-a-cellular-phone
#19
Praveen Rahi, Rashmi Kurli, Mitesh Khairnar, Shubhangi Jagtap, Aabeejjeet N Pansare, Syed G Dastager, Yogesh S Shouche
A novel bacterial strain, designated S5H2222T, was isolated form the screen of a cellular phone. The cells were Gram-stain-positive, rod-shaped, aerobic and motile, and endospores are formed. S5H2222T grew as pale white colonies on trypticase soy agar and the best growth was observed at 37 °C (10-55 °C) and at pH 7.0 (5.0-9.0). S5H2222T could tolerate up to 10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within the genus Lysinibacillus and it exhibited high 16S rRNA gene sequence similarity to Lysinibacillus halotolerans LAM612T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699865/phaeobacter-porticola-sp-nov-an-antibiotic-producing-bacterium-isolated-from-a-sea-harbour
#20
Sven Breider, Heike M Freese, Cathrin Spröer, Meinhard Simon, Jörg Overmann, Thorsten Brinkhoff
Three heterotrophic, aerobic, brown-pigmented strains, designated P97T, P100 and P104, were isolated from a harbour in the southern North Sea. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates are affiliated to the genus Phaeobacter. In silico DNA-DNA hybridization of the genome of strain P97T against those of existing type species indicated that P97T represents a novel species within the genus Phaeobacter, with Phaeobacter inhibens T5T as the closest described organism (29.6 % DNA-DNA relatedness) followed by P...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
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