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https://www.readbyqxmd.com/read/28815948/flow-cytometric-analysis-of-viable-bacteria-in-urine-samples-of-febrile-patients-at-the-emergency-department
#1
Anne H Tavenier, Foppie J de Boer, Bijan Moshaver, Sjef J C M van der Leur, Coen A Stegeman, Paul H P Groeneveld
BACKGROUND: Fast and reliable diagnostics are important in febrile patients admitted to the emergency department. Current urine diagnostics are fast but moderately reliable or reliable but time consuming. Flow cytometry (FC) is a new promising technique in the diagnostics of complicated urinary tract infections by counting bacteria in urine samples. The aim of this study is to improve the FC method by counting only viable bacteria. METHODS: Urine was obtained from 135 consecutive febrile patients at the emergency department...
August 16, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28813773/a-case-of-ivig-induced-aseptic-chemical-meningitis
#2
Anish Patel, Kalyan Chakravarthy Potu, Tamera Sturm
Intravenous immunoglobulin (IVIG) is a commonly used and generally well-tolerated medication. Common side effects include flu-like symptoms such as fevers, headaches, myalgia, fatigue, and nausea. One of the more rare side effects is aseptic meningitis, with a reported incidence rate of around 0.067 percent of all IVIG infusions. In this paper, we describe a 47-year-old female patient with a history of myasthenia gravis who presented with a headache, neck pain, and neck stiffness while undergoing IVIG infusions for a myasthenia crisis...
March 2017: South Dakota Medicine: the Journal of the South Dakota State Medical Association
https://www.readbyqxmd.com/read/28809149/cumulibacter-manganitolerans-gen-nov-sp-nov-isolated-from-sludge-of-a-manganese-mine
#3
Jing Huang, Jiahong Li, Min Cao, Shuijiao Liao, Gejiao Wang
A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8 % similarity), Blastococcus saxobsidens BC444T (93.1 %) and Geodermatophilus aquaeductus BMG801T (92.8 %). It contained iso-C16 : 0, iso-C15 : 0, C17 : 1ω6c, iso-C14 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) as the major fatty acids (>5 %), with MK-9(H4), MK-8(H4) and MK-9(H6) as the quinones...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809148/flaviaesturariibacter-terrae-sp-nov-isolated-from-mountain-soil
#4
Joseph Okiria, Jae-Jin Lee, Su-Jin Park, Yeon-Hee Lee, Seung-Yeol Lee, Sangkyu Park, Byung-Oh Kim, Leonid N Ten, Hee-Young Jung
A Gram-stain-negative, aerobic, rod-shaped, non-motile and pale yellow-pigmented bacterial strain, designated as HY03T, was isolated from mountain soil. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HY03T belonged to the family Chitinophagaceae in the phylum Bacteroidetes and was most closely related to Flaviaesturariibacter amylovorans GCR0105T at a similarity of 95.4 %. The genomic DNA G+C content of strain HY03T was 43.2 mol%. The major fatty acids of the isolate were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809147/flavobacterium-flaviflagrans-sp-nov-a-bacterium-of-the-family-flavobacteriaceae-isolated-from-forest-soil
#5
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Two strains of bacteria designated CB-3T and CB-31 were isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. They were able to grow at 15-42 °C, pH 5.5-9.5 and with 0-1.5 % (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains CB-3T and CB-31 formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes that was distinct from various species of the genus Flavobacterium, including Flavobacterium humicola UCM-46T (99...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809144/defluviimonas-nitratireducens-sp-nov-isolated-from-surface-seawater
#6
Yang Liu, Qiliang Lai, Wanpeng Wang, Zongze Shao
A bacterial strain, designated DL5-4T, was isolated from the surface seawater of Dalian Bay and characterized using a polyphasic taxonomy approach. Cells of DL5-4T were Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 8-40 °C (optimum 28-30 °C), at pH 6-9 (optimum pH 7) and in 0-7 % NaCl (optimum 1-3 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that DL5-4T formed an independent branch with members of the genus Defluviimonas, sharing high similarities with five related type strains, Defluviimonas aquaemixtae CDM-7T (96...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809142/massilia-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#7
Dhiraj Kumar Chaudhary, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, motile and rod-shaped bacterium, designated strain K-3-1T, was isolated from reclaimed grassland soils of Belbari, Morang, Nepal. It was able to grow at 4-45 °C, at pH 5.0-10.0, and at 0-2 % (w/v) NaCl concentrations. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain K-3-1T belongs to the genus Massilia and is closely related to Massilia consociata CCUG 58010T (98.3 % sequence similarity), Massilia tieshanensis TS3T (98...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809140/planococcus-ruber-sp-nov-isolated-from-a-polluted-farmland-soil-sample
#8
Xu Wang, Zhi Wang, Xueqiu Zhao, Xing Huang, Yu Zhou, Wen-Jun Li
A pale-red-pigmented bacterial strain, designated CW1T, was isolated from a polluted soil sample in China and was characterized by a polyphasic taxonomic approach. Strain CW1T was Gram-stain-positive (or variable), coccoid, motile by a single polar flagellum and non-spore-forming. Growth was observed at 15-37 °C, but not at 10 °C or 40 °C, at pH 5.0-9.0 and with 0-5 % NaCl (w/v). Phylogenetic analysis showed that strain CW1T belongs to the genus Planococcus. The 16S rRNA gene sequence similarities between CW1T and the four most closely related type strains, Planococcus antarcticus DSM 14505T, Planococcus halocryophilus DSM 24743T, Planomicrobium soli XN13T and Planomicrobium okeanokoites NBRC 12536T were 97...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809139/paraphaeobacter-pallidus-gen-nov-sp-nov-isolated-from-seawater
#9
Xiulei Cai, Yanan Wang, Xiaoting Yang, Ji Liu, Yanhong Wu, Xiao-Hua Zhang
A Gram-stain-negative, aerobic, rod-shaped bacterium with a single polar flagellum, designated strain DCSW07T, was isolated from the surface water of the Bohai Sea, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DCSW07T shared highest similarity (96.97 %) with Phaeobacter gallaeciensis DSM 26640T, formed a lineage within the family Rhodobacteraceae and was distinct from the most closely related genera Phaeobacter and Pseudooceanicola (96.6-96.8 and 95.8-96.2 % 16S rRNA gene sequence similarity, respectively)...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809138/daejeonia-ginsenosidivorans-gen-nov-sp-nov-a-ginsenoside-transforming-bacterium-isolated-from-lake-water
#10
Muhammad Zubair Siddiqi, Gyu-Min Choi, Mi-Sun Kim, Wan-Taek Im
A Gram-stain-negative, oxidase- and catalase-positive, strictly aerobic, non-gliding bacterial strain, designated as NPT, was isolated from lake water and subjected to a taxonomic study using a polyphasic approach. Colonies of strain NP5T were light-yellow-coloured, circular with regular margins, and opaque. Cells were rods, 0.2-0.5 µm wide and 1.2-3.0 µm long. Strain NP5T possessed β-glycosidase activity, which was responsible for its ability to convert ginsenosides Rb1, Rc and Rd (three main active components of ginseng) to ginsenoside F2...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809137/paracoccus-mangrovi-sp-nov-isolated-from-a-mangrove
#11
Wen-Ming Chen, Yi-Shu Li, Chiu-Chung Young, Shih-Yi Sheu
A bacterial strain, designated gyp-1T, was isolated from a mangrove in Taiwan and characterized using the polyphasic taxonomic approach. Cells of gyp-1T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, coccoid or short-rod-shaped and formed cream-coloured colonies. Growth occurred at 15-37 °C (optimum, 25-30 °C), at pH 5.5-7.0 (optimum, pH 6.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences indicated that gyp-1T represented a member of the genus Paracoccus and showed the highest levels of sequence similarity with respect to Paracoccus lutimaris HDM-25T (97...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28807094/mesonia-maritimus-sp-nov-isolated-from-seawater-of-the-south-sea-of-korea
#12
Hye-Ri Sung, Kiseong Joh, Kee-Sun Shin
A yellow-pigmented bacterial strain, originally designated 15-S14-6T, was isolated from the southern coastal waters of Korea and was subjected to taxonomic study using a polyphasic approach. Strain 15-S14-6T was Gram-staining-negative, flagellated, rod-shaped and aerobic. The strain grew optimally at 25-30 °C, in the presence of 2 % (w/v) NaCl and at pH 7.5-8.0. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that 15-S14-6T represented a member of the genus Mesonia and exhibited the highest sequence similarity (98...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28806629/aseptic-meningitis-in-adults-and-children-diagnostic-and-management-challenges
#13
Bhavarth Shukla, Elizabeth A Aguilera, Lucrecia Salazar, Susan H Wootton, Quanhathai Kaewpoowat, Rodrigo Hasbun
BACKGROUND: Aseptic meningitis represents a common diagnostic and management dilemma to clinicians. OBJECTIVES: To compare the clinical epidemiology, diagnostic evaluations, management, and outcomes between adults and children with aseptic meningitis. STUDY DESIGN: We conducted a retrospective study from January 2005 through September 2010 at 9 Memorial Hermann Hospitals in Houston, TX. Patients age≥2months who presented with community-acquired aseptic meningitis with a CSF white blood cell count >5cells/mm(3) and a negative Gram stain and cultures were enrolled...
August 4, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28799523/bactericidal-activity-of-the-ti-13nb-13zr-alloy-against-different-species-of-bacteria-related-with-implant-infection
#14
John-Jairo Aguilera-Correa, Ana Conde, Maria-Angeles Arenas, Juan-Jose de-Damborenea, Miguel Marin, Antonio L Doadrio, Jaime Esteban
The Ti-6Al-4V alloy is one of the most commonly used in orthopedic surgery. Despite its advantages, there is an increasing need to use new titanium alloys with no toxic elements and improved biomechanical properties, such as Ti-13Nb-13Zr. Prosthetic joint infections (PJI) are mainly caused by Gram-positive bacteria; however, Gram-negative bacteria are a growing problem due to associated multidrug resistance. In this study, the bacterial adherence and viability on the Ti-13Nb-13Zr alloy have been compared to that of the Ti-6Al-4V alloy using 16 collection and clinical strains of bacterial species related to PJI: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa...
August 11, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28799031/spirosoma-metallum-sp-nov-isolated-from-an-automobile-air-conditioning-system
#15
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0653(T), was isolated from an automobile evaporator core collected in Korea. The cells were aerobic and rod-shaped. The strain grew at 10-28 °C (optimum, 25 °C), at pH 6.0-7.5 (optimum, 6.5), and in the presence of 0-1% (w/v) NaCl (optimum, 0%). Phylogenetically, the strain was related to members of the genus Spirosoma (95.1-90.8% 16S rRNA sequence similarity) and distantly related to Spirosoma pulveris JSH5-14(T) (95.1%), Spirosoma fluviale MSd3(T) (95...
August 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28798534/a-case-of-spontaneous-purulent-granulomatous-pericarditis-in-a-beagle
#16
Kyohei Kamio, Yutaka Nakanishi, Kenta Matsue, Minoru Sasaki
The present report describes a case of spontaneous purulent granulomatous pericarditis in a 16-month-old beagle. A gross necropsy revealed pericardial effusion and multiple nodules on the surface of the heart and around the aorta adjacent to the heart. The cut surface of these nodules was solid and white in color, containing partially yellowish white regions. Microscopically, granulomatous inflammation characterized by central necrotic cellular debris surrounded by neutrophils, macrophages, lymphocytes, plasma cells, fibroblasts and collagen fibers was observed in the epicardium...
July 2017: Journal of Toxicologic Pathology
https://www.readbyqxmd.com/read/28796148/pain-and-antisepsis-after-ocular-administration-of-povidone-iodine-versus-chlorhexidine
#17
Carmen Oakley, Penny Allen, Joobin Hooshmand, Brendan J T Vote
PURPOSE: To investigate ocular bacterial count before and after antisepsis with aqueous chlorhexidine (AC) or povidone-iodine (PI) and to assess discomfort with each agent. METHODS: Bacterial swabs were taken from participants' eyes before and after antisepsis. These underwent microscopy, culture, and sensitivity testing. Aqueous chlorhexidine drops were administered to left eyes and PI to right eyes. Participants rated their pain (scale 0-10) for each eye but were blinded to the type of drop...
August 8, 2017: Retina
https://www.readbyqxmd.com/read/28795380/peritoneal-dialysis-associated-catheter-infection-caused-by-mycobacterium-abscessus-in-an-elderly-patient-who-was-successfully-treated-with-catheter-removal
#18
Arata Hibi, Takahisa Kasugai, Keisuke Kamiya, Keisuke Kamiya, Chiharu Ito, Satoru Kominato, Ken Mizuguchi, Toshiyuki Miura, Katsushi Koyama
An 89-year-old Japanese man on peritoneal dialysis (PD) was suspected of having a PD-associated catheter infection. He visited the hospital because of the discharge of pus from the exit site of his catheter. Gram staining of the pus showed Gram-positive bacilli, but these were acid-fast bacilli. The rapidly growing nontuberculous mycobacteria, Mycobacterium abscessus, was isolated. PD catheter removal and debridement were immediately performed. The patient received combination antibiotic therapy. His clinical course was good, but he required hemodialysis due to the discontinuation of PD...
August 9, 2017: CEN Case Reports
https://www.readbyqxmd.com/read/28792377/leucobacter-ruminantium-sp-nov-isolated-from-the-bovine-rumen
#19
Byung Hee Chun, Hyo Jung Lee, Sang Eun Jeong, Peter Schumann, Che Ok Jeon
A Gram-stain-positive, lemon yellow-pigmented, non-motile, rod-shaped bacterium, designated strain A2T, was isolated from the rumen of cow. Cells were catalase-positive and weakly oxidase-positive. Growth of strain A2T was observed at 25-45 °C (optimum, 37-40 °C), at pH 5.5-9.5 (optimum, pH 7.5) and in the presence of 0-3.5 % (w/v) NaCl (optimum, 1 %). Strain A2T contained iso-C16 : 0 and anteiso-C15 : 0 as the major cellular fatty acids. Menaquinone-11 was detected as the sole respiratory quinone...
August 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28792375/sphingomonas-olei-sp-nov-with-the-ability-to-degrade-aliphatic-hydrocarbons-isolated-from-oil-contaminated-soil
#20
Dhiraj Kumar Chaudhary, Jaisoo Kim
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped bacterium, designated K-1-16T, that is capable of degrading aliphatic hydrocarbons was isolated from oil-contaminated soil at Biratnagar, Morang, Nepal. It was able to grow at 15-45 °C, at pH 5.5-9.5 and with 0-5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-1-16T belongs to the genus Sphingomonas and is closely related to Sphingomonas mucosissima CP173-2T (98...
August 10, 2017: International Journal of Systematic and Evolutionary Microbiology
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