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https://www.readbyqxmd.com/read/29144232/saccharopolyspora-aidingensis-sp-nov-an-actinomycete-isolated-from-a-salt-lake
#1
Zhan-Feng Xia, Xiao-Xia Luo, Chuan-Xing Wan, Li-Li Zhang
A novel halophilic actinomycete strain, designated TRM 46074(T), was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074(T) was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose...
March 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29143729/thalassotalea-coralli-sp-nov-isolated-from-the-torch-coral-euphyllia-glabrescens
#2
Der-Shyan Sheu, Shih-Yi Sheu, Pei-Bei Xie, Sen-Lin Tang, Wen-Ming Chen
Strain Eup a-8(T), isolated from a torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-8(T) were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly-β-hydroxybutyrate-containing, rod-shaped and formed white colonies. Optimal growth occurred at 25-30 °C, pH 7-8, and in the presence of 2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-8(T) belonged to the genus Thalassotalea and showed the highest levels of sequence similarity with respect to Thalassotalea ganghwensis JC2041(T) (97...
November 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29143725/thalassotalea-atypica-sp-nov-isolated-from-seawater-and-emended-description-of-thalassotalea-eurytherma
#3
Yan Wang, Tingwei Liu, Hong Ming, Pengyang Sun, Chenxi Cao, Menglong Guo, Junran Du, Chenyan Zhou, Wuling Zhu
A novel Gram-stain-negative, straight or slightly curved rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated RZG4-3-1(T), was isolated from coastal seawater of Rizhao, China (119.625° E 35.517° N). The organism grew optimally at 24-28 °C, at pH 7.0 and in the presence of 2.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZG4-3-1(T) contained ubiquinone 8 (Q-8) as the major respiratory quinone and contained C16 : 1ω7c and/or C16 : 1ω6c and C16 : 0 as the dominant fatty acids...
November 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29141775/impact-of-interventions-by-an-antimicrobial-stewardship-program-team-on-appropriate-antimicrobial-therapy-in-patients-with-bacteremic-urinary-tract-infection
#4
Mitsuru Yanai, Maiko Ogasawara, Yuta Hayashi, Kiyozumi Suzuki, Hiromichi Takahashi, Atsushi Satomura
BACKGROUND: Inappropriate antimicrobial therapy often leads to poor outcomes. This study aimed to evaluate the impact of an antimicrobial stewardship program (ASP) team on appropriate therapy, in patients with bacteremic urinary tract infection (UTI). PATIENTS AND METHODS: We retrospectively reviewed the interventions by the ASP team in 807 patients with bacteremic UTI. Interventions were divided into 3 groups: group A (conventional report), group B (conventional report and written alert on the chart), and group C (conventional report and oral recommendation with/without written alert)...
November 12, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29139350/nemorella-caseinilytica-gen-nov-sp-nov-isolated-from-forest-soil
#5
Dhiraj Kumar Chaudhary, Ram Hari Dahal, Jaisoo Kim
Two novel strains, J116-2(T) and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2(T) and J116-1 were able to grow at 20-32 °C, pH 6.0-8.5, and 0-0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2(T) and J116-1 formed a distinct lineage within the family Chitinophagaceae of the phylum Bacteroidetes and were closely related to genera Taibaiella (89...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29139349/leucobacter-triazinivorans-sp-nov-a-s-triazine-herbicide-prometryn-degrading-bacterium-isolated-from-sludge
#6
Le-Ni Sun, Dan-Dan Pan, Xiang-Wei Wu, En-Dong Yang, Ri-Mao Hua, Qing X Li
A Gram-stain-positive, rod-shaped, non-motile bacterial strain, designated JW-1(T), was isolated from activated sludge collected from the outlet of an aeration tank in a prometryn-manufacturing plant, located in Binzhou City, Shandong province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain JW-1(T) belongs to the genus Leucobacter and its closest neighbours are 'Leucobacter kyeonggiensis' F3-P9 (98.95 % similarity), Leucobacter celer subsp. astrifaciens CBX151(T) (98...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29139347/aestuariicoccus-marinus-gen-nov-sp-nov-isolated-from-sea-tidal-flat-sediment
#7
Tingye Feng, Sang Eun Jeong, Kyung Hyun Kim, Hye Yoon Park, Che Ok Jeon
A Gram-stain-negative, strictly aerobic and halotolerant bacterial strain, designated strain NAP41(T), was isolated from a sea tidal flat in the Yellow Sea of South Korea. Cells were non-motile cocci showing oxidase- and catalase-positive activities. Growth of strain NAP41(T) was observed at 15-40 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, pH 7.0-7.5) and in the presence of 0.5-12 % (w/v) NaCl (optimum, 2 %). Strain NAP41(T) contained summed feature 8 (comprising C18 : ω7c/C18 : 1ω6c) and C18 : 0 as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29139002/deinococcus-multiflagellatus-sp-nov-isolated-from-a-car-air-conditioning-system
#8
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, Lei Zhao, Min-Kyu Kim, Jae-Hyung Ahn, Jong-Ok Ka
A gamma radiation-resistant and pink-to-red pigmented bacterial strain, designated ID1504(T), was isolated from a car air-conditioning system sampled in Korea. The cells were observed to be Gram-stain negative, aerobic, motile with peritrichous flagella and short rod-shaped. Phylogenetically, the strain groups with the members of the genus Deinococcus and exhibits high 16S rRNA gene sequence similarities with Deinococcus arenae SA1(T) (94.0%), Deinococcus actinosclerus BM2(T) (93.9%) and Deinococcus soli N5(T) (93...
November 14, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29135992/internal-quality-assurance-in-diagnostic-microbiology-a-simple-approach-for-insightful-data
#9
Valentin Scherz, Christian Durussel, Gilbert Greub
Given the importance of microbiology results on patient care, high quality standards are expected. Internal quality assurance (IQA) could mitigate the limitations of internal quality control, competency assessment and external quality assurance, adding a longitudinal insight, including pre- and post-analytical steps. Here, we implemented an IQA program in our clinical microbiology facilities with blind resubmission of routine samples during 22 months. One-hundred-and-twenty-one out of 123 (98.4%) serological analyses and 112 out of 122 (91...
2017: PloS One
https://www.readbyqxmd.com/read/29135519/syphilis-of-the-aerodigestive-tract
#10
Julie Y Tse, May P Chan, Judith A Ferry, Vikram Deshpande, Aliyah R Sohani, Valentina Nardi, Andras Schaffer, Rosalynn M Nazarian, Lawrence R Zukerberg
Syphilis, a sexually transmitted infection caused by the Gram-negative bacterium Treponema pallidum, is increasing in prevalence in the United States. It has been our experience that primary and secondary syphilis of the aerodigestive tract can afflict a large age spectrum with varied clinical and histopathologic findings, which can lead to diagnostic problems and frequent misdiagnosis. In this study, we describe the histopathologic patterns of syphilis of the aerodigestive tract to expand awareness of its varied appearance...
November 9, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29134941/bacillus-marinisedimentorum-sp-nov-isolated-from-marine-sediment
#11
Ling-Yun Guo, Si-Kai Ling, Chang-Ming Li, Guan-Jun Chen, Zong-Jun Du
A novel Gram-stain-positive, motile and facultatively anaerobic strain, designated NC2-31(T), was isolated from sediment from the coast of Weihai, PR China. Optimal growth occurred at 37 °C, pH 7.5 and with 2.0-3.0 % (w/v) NaCl. MK-7 was the major respiratory quinone. Meso-diaminopimelic acid was a diagnostic diamino acid in the peptidoglycan. The major polar lipids of NC2-31(T) were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The genomic DNA G+C content of the strain was 46...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29134936/paenibacillus-xanthanilyticus-sp-nov-a-xanthan-degrading-bacterium-isolated-from-soil
#12
Simin Ashraf, Mohammad Reza Soudi, Mohammad Ali Amoozegar, Mahdi Moshtaghi Nikou, Cathrin Spröer
A xanthan-degrading bacterium, strain AS7(T), was isolated from soil and its taxonomic position was determined using a polyphasic approach. Strain AS7(T) was a Gram-stain-variable, spore-forming, motile, aerobic, rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that strain AS7(T) belongs to the genus Paenibacillus, sharing the highest level of sequence similarity with Paenibacillus phyllosphaerae PALXIL04(T) (98.0 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29134934/paenibacillus-shunpengii-sp-nov-isolated-from-farmland-soil
#13
You-Jian Yang, Yan-Ting Zhang, Guo-Qiang Chen, Dan Cheng, Ji-Guo Qiu, Qin He, Jian He
A bacterial strain designated YYJ7-1(T) was isolated from farmland soil in China and characterized using a polyphasic taxonomic approach. Cells of strain YYJ7-1(T) were Gram-staining-positive, aerobic or facultatively anaerobic, rod-shaped, motile and endospore-forming. Growth occurred at 18-42 °C (optimum at 35 °C), at pH 6.0-8.0 (optimum at pH 7.5) and with 0.0-4.0 % NaCl (optimum with 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus and showed high levels of sequence similarity with respect to Paenibacillus provencensis 4401170(T) (98...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29134394/polyphasic-taxonomic-characterisation-of-a-novel-strain-as-pararhizobium-haloflavum-sp-nov-isolated-from-soil-samples-near-a-sewage-treatment-tank
#14
Xia Shen, Yu Li, Zhe Zhao, Yi-Fan Han, Wen-Wu Zhang, Xiao-Yun Yu, Chong-Ya Zhang, Cong Sun, Min Wu
A Gram-stain negative, aerobic, motile and ovoid- to rod-shaped bacteria strain, designated XC0140(T), was isolated from soil samples near the sewage treatment tank of a chemical factory in Zhejiang Province, China, and subjected to polyphasic taxonomic investigation. Strain XC0140(T) grew at 10-37 °C and pH 6.0-9.0 (optimum, 35 °C and pH 7.5) and with 0-17% (w/v) NaCl (optimum, 1%). According to phylogenetic analysis based on 16S rRNA gene sequences, strain XC0140(T) was assigned to the genus Pararhizobium with high 16S rRNA gene sequence similarity of 95...
November 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29131371/utility-of-gram-staining-for-diagnosis-of-malassezia-folliculitis
#15
Wei-Ting Tu, Szu-Ying Chin, Chia-Lun Chou, Che-Yuan Hsu, Yu-Tsung Chen, Donald Liu, Woan-Ruoh Lee, Yi-Hsien Shih
Malassezia folliculitis (MalF) mimics acne vulgaris and bacterial folliculitis in clinical presentations. The role of Gram staining in rapid diagnosis of MalF has not been well studied. In our study, 32 patients were included to investigate the utility of Gram staining for MalF diagnosis. The final diagnoses of MalF were determined according to clinical presentation, pathological result and treatment response to antifungal agents. Our results show that the sensitivity and specificity of Gram staining are 84...
November 13, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/29130429/izhakiella-australiensis-sp-nov-isolated-from-an-australian-desert-soil
#16
Mukan Ji, Sihui Tang, Belinda C Ferrari
A Gram-stain-negative, rod-shaped, non-motile bacterium, designated D4N98(T), was isolated from a desert soil near Glendambo, Australia. The taxonomic position of strain D4N98(T) was investigated using a polyphasic approach. Strain D4N98(T) shared 97 % 16S rRNA gene sequence similarity with the only reference strain of the genus Izhakiella (Izhakiella capsodis N6PO6(T)), and less than 96 % similarity with other species of genera in the family Enterobacteriaceae with validly published names. Phylogenetic analysis, based on 16S rRNA, rpoB, atpD, gyrB genes and a concatenated sequence comprising 37 single copy marker genes, as well as chemotaxonomic data (major polar lipids: phosphatidyl ethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid, and a glycolipid...
November 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29125459/pseudogracilibacillus-endophyticus-sp-nov-a-moderately-thermophilic-and-halophilic-species-isolated-from-plant-root
#17
Jisun Park, Min-Kyeong Kim, Bo-Ram Yun, Ji-Hye Han, Seung Bum Kim
A Gram-stain-positive strain, designated DT7-02(T), was isolated from the surface-sterilized root of Oenotherabiennis (evening primrose) and subjected to taxonomic characterization. Cells of DT7-02(T) were slender rod-shaped, motile by means of flagella, and oxidase- and catalase-positive. The colonies were circular, pinkish-yellow, opaque, glistering and 1-2 mm in diameter. The strain was moderately thermophilic and halophilic, as growth occurred at 20-44 °C (optimum 40 °C), pH 7-10 (optimum pH 8-9) and in the presence of 0-8 % of NaCl (optimum 4 %) in tryptic soy broth...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29125456/microbacterium-album-sp-nov-and-microbacterium-deserti-sp-nov-two-halotolerant-actinobacteria-isolated-from-desert-soil
#18
Zi-Wen Yang, Nimaichand Salam, Mohamed Mohany, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Min Xiao, Wael N Hozzein, Wen-Jun Li
Strains SYSU D8007(T) and SYSU D8014(T) were isolated from desert soil collected from Saudi Arabia. The two isolates were Gram-stain-positive, non-motile, aerobic and non-spore-forming. These strains were able to grow at 4-45 °C and in the presence of up to 8 % (w/v) NaCl. Strain SYSU D8007(T) could grow at pH 6.0-10.0, and strain SYSU D8014(T) at pH 5.0-10.0. They shared highest 16S rRNA gene sequence similarities with Microbacterium marinilacus YM11-607(T) and Microbacterium paludicola US15(T). Menaquinones MK-11 and MK-12 were detected as the respiratory quinones...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29123908/a-case-of-disseminated-cryptococcosis-with-necrotizing-fasciitis-in-a-non-hiv-patient
#19
Tetsuya Hoshino, Kazuya Omura, Shinichi Kimura, Hiroyuki Takahashi, Katsuhiko Kamei, Misako Ohkusu
Case: Disseminated cryptococcosis is a well-recognized condition among HIV patients, but it also occurs in non-HIV patients. Necrotizing fasciitis caused by cryptococcus is rare. An 81-year-old man who had received steroid therapy presented with erythema and pain in his right thigh. After the rapid progression of symptoms and a failure to respond to antibiotic therapy, a clinical diagnosis of necrotizing fasciitis was made. We performed debridement, and yeasts were detected using a Gram stain of the fascia...
October 2017: Acute Medicine & Surgery
https://www.readbyqxmd.com/read/29121996/production-and-characterization-of-monoclonal-antibodies-against-encephalitozoon-intestinalis-and-encephalitozoon-sp-spores-and-their-developmental-stages
#20
Fernando Izquierdo, Hercules Moura, Fernando Jorge Bornay-Llinares, Rama Sriram, Carolina Hurtado, Ángela Magnet, Soledad Fenoy, Govinda Visvesvara, Carmen Del Aguila
BACKGROUND: Microsporidia are intracellular obligate parasites traditionally associated with immunosuppressed patients; their detection in immunocompetent patients has increased, highlighting their possible importance as emerging pathogens. Detection of spores in stools, urine, body fluids and tissues is difficult and immunological techniques such as immunofluorescence have proved to be a useful and reliable tool in the diagnosis of human microsporidiosis. For this reason, we have produced and characterized monoclonal antibodies (MAbs) specific for Encephalitozoon intestinalis (the second most frequent microsporidian infecting humans), and other Encephalitozoon species, that can be used in different diagnostic techniques...
November 9, 2017: Parasites & Vectors
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