Tian-Fu He, Huan-Hui Zhu, Xian-Wen Lin, Yuan-Yuan Tian, Li-Min Sun, Xu Guan, Hai-Yan Zhang, Li Tan, Song-Cai Wang
Etomidate (ETO) is a highly-efficient drug that can induce anesthesia with increasing doses, thus subject to strict regulation. However, an accurate and efficient method for ETO intake detection is currently lacking. Therefore, this study developed a straightforward sample preparation method using LC-MS/MS to analyze ETO and its primary metabolite, etomidate acid (ETA), in urine, liver, and kidney samples. Snap frozen pig liver and kidney samples were ground into a fine powder. Then, all the biological samples, including human urine, pig liver and kidney tissues, were deproteinized using acetonitrile and filtered for analysis...
December 21, 2023: Journal of Pharmacological and Toxicological Methods