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https://www.readbyqxmd.com/read/28535377/mechanism-of-ska-recruitment-by-ndc80-complexes-to-kinetochores
#1
Paweł Ł Janczyk, Katarzyna A Skorupka, John G Tooley, Daniel R Matson, Cortney A Kestner, Thomas West, Owen Pornillos, P Todd Stukenberg
Yeast use the ring-shaped Dam1 complex to slide down depolymerizing microtubules to move chromosomes, but current models suggest that other eukaryotes do not have a sliding ring. We visualized Ndc80 and Ska complexes on microtubules by electron microscopic tomography to identify the structure of the human kinetochore-microtubule attachment. Ndc80 recruits the Ska complex so that the V shape of the Ska dimer interacts along protofilaments. We identify a mutant of the Ndc80 tail that is deficient in Ska recruitment to kinetochores and in orienting Ska along protofilaments in vitro...
May 22, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28533102/real-time-electronic-dashboard-technology-and-its-use-to-improve-pediatric-radiology-workflow
#2
REVIEW
Randheer Shailam, Ariel Botwin, Markus Stout, Michael S Gee
The purpose of our study was to create a real-time electronic dashboard in the pediatric radiology reading room providing a visual display of updated information regarding scheduled and in-progress radiology examinations that could help radiologists to improve clinical workflow and efficiency. To accomplish this, a script was set up to automatically send real-time HL7 messages from the radiology information system (Epic Systems, Verona, WI) to an Iguana Interface engine, with relevant data regarding examinations stored in an SQL Server database for visual display on the dashboard...
March 19, 2017: Current Problems in Diagnostic Radiology
https://www.readbyqxmd.com/read/28532015/effect-of-sintering-temperature-rise-from-870-to-920%C3%A2-c-on-physicomechanical-and-biological-quality-of-nano-hydroxyapatite-an-explorative-multi-phase-experimental-in-vitro-vivo-study
#3
Ahad Khoshzaban, Vahid Rakhshan, Farhood Najafi, Leila Aghajanpour, Seyed Jafar Hashemian, Saeed Heidari Keshel, Ikuya Watanabe, Alireza Valanezhad, Tahereh Sadat Jafarzadeh Kashi
Hydroxyapatite (HA) is a proper scaffold for bone repair, however, it is not of excellent mechanical properties. Most previous studies on the effect of temperature increases were in vitro and had assessed merely improvements of HA's physicomechanical quality. This in vitro/vivo study investigated the effect of temperature increases from 870 to 920°C on physicomechanical and biological quality of Nano-HA. Forty experimentally produced HA disks sintered at 870 to 920°C were prepared (n=20×2). Disks were subjected to Vickers microindentation test (1 disk from each group divided into 4 quarters), Fourier transform infrared spectroscopy (1 disk), X-ray diffraction (XRD) [1 disk together with non-sintered HA], field emission scanning electron microscopy (FSEM, 1 disk from each group together with non-sintered HA), cell seeding and SEM assessment (2 disks), MTT assay over 4 different time periods (16 quadrants of 4 disks from each group), 6 one-thirds of 2 disks from each group for immunocytochemical (ICC) assay, and 8 disks from each group [as well as non-sintered HA] for the animal study (implantation in 4 sockets in 8 rabbits [32 specimens], histomorphometry, and computerized tomography) over two time periods...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28528642/minimal-resin-embedding-of-multicellular-specimens-for-targeted-fib-sem-imaging
#4
Nicole L Schieber, Pedro Machado, Sebastian M Markert, Christian Stigloher, Yannick Schwab, Anna M Steyer
Correlative light and electron microscopy (CLEM) is a powerful tool to perform ultrastructural analysis of targeted tissues or cells. The large field of view of the light microscope (LM) enables quick and efficient surveys of the whole specimen. It is also compatible with live imaging, giving access to functional assays. CLEM protocols take advantage of the features to efficiently retrace the position of targeted sites when switching from one modality to the other. They more often rely on anatomical cues that are visible both by light and electron microscopy...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528637/find-your-way-with-x-ray-using-microct-to-correlate-in-vivo-imaging-with-3d-electron-microscopy
#5
Matthia A Karreman, Bernhard Ruthensteiner, Luc Mercier, Nicole L Schieber, Gergely Solecki, Frank Winkler, Jacky G Goetz, Yannick Schwab
Combining in vivo imaging with electron microscopy (EM) uniquely allows monitoring rare and critical events in living tissue, followed by their high-resolution visualization in their native context. A major hurdle, however, is to keep track of the region of interest (ROI) when moving from intravital microscopy (IVM) to EM. Here, we present a workflow that relies on correlating IVM and microscopic X-ray computed tomography to predict the position of the ROI inside the EM-processed sample. The ROI can then be accurately and quickly targeted using ultramicrotomy and imaged using EM...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528634/3d-subcellular-localization-with-superresolution-array-tomography-on-ultrathin-sections-of-various-species
#6
Sebastian M Markert, Vivien Bauer, Thomas S Muenz, Nicola G Jones, Frederik Helmprobst, Sebastian Britz, Markus Sauer, Wolfgang Rössler, Markus Engstler, Christian Stigloher
Array Tomography (AT) is a relatively easy-to-use and yet powerful method to put molecular identity in its full ultrastructural context. Ultrathin sections are stained with fluorophores and then imaged by light and afterward by electron microscopy to obtain a correlated view of a region of interest: its ultrastructure and specific staining. By combining AT with high-pressure freezing for superior structural preservation and superresolution light microscopy, even small subcellular structures can be mapped in 3D...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528628/millisecond-time-resolution-correlative-light-and-electron-microscopy-for-dynamic-cellular-processes
#7
Ludek Stepanek, Gaia Pigino
Molecular motors propel cellular components at velocities up to microns per second with nanometer precision. Imaging techniques combining high temporal and spatial resolution are therefore indispensable to understand the cellular mechanics at the molecular level. For example, intraflagellar transport (IFT) trains constantly shuttle ciliary components between the base and tip of the eukaryotic cilium. 3-D electron microscopy has revealed IFT train morphology and position, but was unable to correlate these features with the direction of train movement...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528370/systematic-radiographic-evaluation-of-three-potential-implantation-sites-for-a-semi-implantable-bone-conduction-device-in-52-patients-after-previous-mastoid-surgery
#8
Bernhard G Weiss, Mattis Bertlich, Rebecka Scheele, Martin Canis, Mark Jakob, Jan M Sohns, Friedrich Ihler
The aim of this study was the evaluation of three localizations for the implantation of a semi-implantable transcutaneous bone conduction device after previous mastoid surgery. This is a retrospective review of electronic datasets of cranial computed tomography studies. The study setting is one tertiary referral center and included 52 consecutive adult patients (60 temporal bones) with a history of mastoid surgery. The intervention was virtual placement of the device with a planning software within the remaining mastoid as well as dorsal of the sigmoid sinus and caudal of the transverse sinus (retrosigmoidal localization) and dorsocranial of the parietomastoid suture and cranial of the transverse sinus (parietal localization)...
May 20, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28526874/radiopaque-resorbable-inferior-vena-cava-filter-infused-with-gold-nanoparticles
#9
Li Tian, Patrick Lee, Burapol Singhana, Aaron Chen, Yang Qiao, Linfeng Lu, Jonathan O Martinez, Ennio Tasciotti, Adam Melancon, Steven Huang, Mitch Eggers, Marites P Melancon
Failure to remove a retrievable inferior vena cava (IVC) filter can cause severe complications with high treatment costs. Polydioxanone (PPDO) has been shown to be a good candidate material for resorbable IVC filters. However, PPDO is radioluscent under conventional imaging modalities. Thus, the positioning and integrity of these PPDO filters cannot be monitored by computed tomography (CT) or x-ray. Here we report the development of radiopaque PPDO IVC filters based on gold nanoparticles (AuNPs). Commercially available PPDO sutures were infused with AuNPs...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526667/patient-centered-decision-support-formative-usability-evaluation-of-integrated-clinical-decision-support-with-a-patient-decision-aid-for-minor-head-injury-in-the-emergency-department
#10
Edward R Melnick, Erik P Hess, George Guo, Maggie Breslin, Kevin Lopez, Anthony J Pavlo, Fuad Abujarad, Seth M Powsner, Lori A Post
BACKGROUND: The Canadian Computed Tomography (CT) Head Rule, a clinical decision rule designed to safely reduce imaging in minor head injury, has been rigorously validated and implemented, and yet expected decreases in CT were unsuccessful. Recent work has identified empathic care as a key component in decreasing CT overuse. Health information technology can hinder the clinician-patient relationship. Patient-centered decision tools to support the clinician-patient relationship are needed to promote evidence-based decisions...
May 19, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28526599/the-impact-of-an-electronic-best-practice-advisory-on-brain-computed-tomography-ordering-in-an-academic-emergency-department
#11
Donald Szlosek, Samir A Haydar, Rachel J Williams, Ryan C Jackson, Christine L Hein, Nathan Mick, Tania D Strout
No abstract text is available yet for this article.
May 15, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28526235/zero-echo-time-mri-only-treatment-planning-for-radiation-therapy-of-brain-tumors-after-resection
#12
C Boydev, B Demol, D Pasquier, H Saint-Jalmes, G Delpon, N Reynaert
Using magnetic resonance imaging (MRI) as the sole imaging modality for patient modeling in radiation therapy (RT) is a challenging task due to the need to derive electron density information from MRI and construct a so-called pseudo-computed tomography (pCT) image. We have previously published a new method to derive pCT images from head T1-weighted (T1-w) MR images using a single-atlas propagation scheme followed by a post hoc correction of the mapped CT numbers using local intensity information. The purpose of this study was to investigate the performance of our method with head zero echo time (ZTE) MR images...
May 16, 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28525260/three-dimensional-visualization-and-characterization-of-polymeric-self-assemblies-by-transmission-electron-microtomography
#13
Hiroshi Jinnai, Takeshi Higuchi, Xiaodong Zhuge, Akihito Kumamoto, Kees Joost Batenburg, Yuichi Ikuhara
Self-assembling structures and their dynamical processes in polymeric systems have been investigated using three-dimensional transmission electron microscopy (3D-TEM). Block copolymers (BCPs) self-assemble into nanoscale periodic structures called microphase-separated structures, a deep understanding of which is important for creating nanomaterials with superior physical properties, such as high-performance membranes with well-defined pore size and high-density data storage media. Because microphase-separated structures have become increasingly complicated with advances in precision polymerization, characterizing these complex morphologies is becoming increasingly difficult...
May 19, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28521841/tnf-inhibitors-appear-to-inhibit-disease-progression-and-improve-outcome-in-takayasu-arteritis-an-observational-population-based-time-trend-study
#14
Birgir Gudbrandsson, Øyvind Molberg, Øyvind Palm
BACKGROUND: Magnetic resonance imaging (MRI) and computed tomography (CT) angiography have now largely replaced interventional angiography in the diagnoses and follow up of Takayasu arteritis (TAK) but data on the effects of this change of imaging method on diagnostic delay and vascular damage, and detailed data on the effect of different treatment regimens on the accumulation of vascular damage are missing. The aim of this study was to assess time trends in diagnostic delay, therapeutic approaches, arterial lesion accrual, persistent disease activity and remission rates in TAK...
May 18, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28513854/verification-of-dose-profiles-generated-by-the-convolution-algorithm-of-the-gamma-knife-%C3%A2-radiosurgery-planning-system
#15
Hyun-Tai Chung, Jeong-Hoon Park, Kook Jin Chun
PURPOSE: A convolution algorithm that takes into account electron-density inhomogeneity was recently introduced to calculate dose distributions for the Gamma Knife (GK) Perfexion(™) treatment planning program. The accuracies of the dose distributions computed using the convolution method were assessed using an anthropomorphic phantom and film dosimetry. METHODS: Absorbed-dose distributions inside a phantom (CIRS Radiosurgery Head Phantom, Model 605) were calculated using the convolution method of the GK treatment-planning software (Leksell Gamma Plan(®) version 10...
May 17, 2017: Medical Physics
https://www.readbyqxmd.com/read/28513613/defects-in-the-acid-phosphatase-acpt-cause-recessive-hypoplastic-amelogenesis-imperfecta
#16
Claire El Smith, Laura LE Whitehouse, James A Poulter, Steven J Brookes, Peter F Day, Francesca Soldani, Jennifer Kirkham, Chris F Inglehearn, Alan J Mighell
We identified two homozygous missense variants (c.428C>T, p.(T143M) and c.746C>T, p.(P249L)) in ACPT, the gene encoding acid phosphatase, testicular, which segregates with hypoplastic amelogenesis imperfecta in two unrelated families. ACPT is reported to play a role in odontoblast differentiation and mineralisation by supplying phosphate during dentine formation. Analysis by computerised tomography and scanning electron microscopy of a primary molar tooth from an individual homozygous for the c.746C>T variant revealed an enamel layer that was hypoplastic, but mineralised with prismatic architecture...
May 17, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28512784/evaluation-of-aromatic-radiobromination-by-nucleophilic-substitution-using-diaryliodonium-salt-precursors
#17
Dong Zhou, Sung Hoon Kim, Wenhua Chu, Thomas Voller, John A Katzenellenbogen
Radiobromine labelled compounds can be used for positron emission tomography (PET) imaging (i.e. (76) Br) and for radiation therapy (i.e. (77) Br). However, the commonly used electrophilic substitution reaction using no-carrier-added (NCA) radiobromide does not always afford the desired product due to the high reactivity of the brominating intermediate. A nucleophilic substitution by bromide, such as radiobromination of iodonium precursors, provides an alternative route for the synthesis of bromo-radiopharmaceuticals...
May 17, 2017: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/28512570/the-structure-of-denisovite-a-fibrous-nanocrystalline-polytypic-disordered-very-complex-silicate-studied-by-a-synergistic-multi-disciplinary-approach-employing-methods-of-electron-crystallography-and-x-ray-powder-diffraction
#18
Ira V Rozhdestvenskaya, Enrico Mugnaioli, Marco Schowalter, Martin U Schmidt, Michael Czank, Wulf Depmeier, Andreas Rosenauer
Denisovite is a rare mineral occurring as aggregates of fibres typically 200-500 nm diameter. It was confirmed as a new mineral in 1984, but important facts about its chemical formula, lattice parameters, symmetry and structure have remained incompletely known since then. Recently obtained results from studies using microprobe analysis, X-ray powder diffraction (XRPD), electron crystallography, modelling and Rietveld refinement will be reported. The electron crystallography methods include transmission electron microscopy (TEM), selected-area electron diffraction (SAED), high-angle annular dark-field imaging (HAADF), high-resolution transmission electron microscopy (HRTEM), precession electron diffraction (PED) and electron diffraction tomography (EDT)...
May 1, 2017: IUCrJ
https://www.readbyqxmd.com/read/28504292/microstructure-degradation-of-ni-cgo-anodes-for-solid-oxide-fuel-cells-after-long-operation-time-using-3d-reconstructions-by-fib-tomography
#19
Atef Zekri, Martin Knipper, Jürgen Parisi, Thorsten Plaggenborg
Solid oxide fuel cells (SOFCs) are electrochemical conversion devices, which essentially consist of two porous electrodes separated by a dense, oxide ion conducting electrolyte. The performance and the durability of SOFCs strongly depend on the electrode microstructure. In this paper, the impact of a relatively long exposure time (up to 20 000 h) under realistic operation terms (temperature (T) = 850 °C, current density (J) = 190-250 mA cm(-2)) in the kinetics of microstructural degradation are investigated for porous nickel (Ni)/ceria gadolinium oxide (CGO) anodes, to understand the microstructural evolution in SOFC cermet anodes...
May 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28501440/classification-of-microcrystalline-celluloses-via-structures-of-individual-particles-measured-by-synchrotron-radiation-x-ray-micro-computed-tomography
#20
Longwei Fang, Xianzhen Yin, Li Wu, Yaping He, Yuanzhi He, Wei Qin, Fanyue Meng, Peter York, Xu Xu, Jiwen Zhang
Microcrystalline cellulose (MCC) is one of the most important excipients due to its outstanding binding and tableting properties. Owing to the absence of high resolution characterization techniques at the single particle scale, 3D (three dimension) microstructure of MCC and its effects on formulation performance remain unexamined. The aim of this work was to establish a methodology for single particles of MCC type 102 based on synchrotron radiation X-ray micro computed tomography (SR-μCT), principal component analysis (PCA) and partial least square discriminant analysis (PLSDA)...
May 10, 2017: International Journal of Pharmaceutics
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