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https://www.readbyqxmd.com/read/28422143/dissecting-the-genetic-architecture-of-fusarium-verticillioides-seed-rot-resistance-in-maize-by-combining-qtl-mapping-and-genome-wide-association-analysis
#1
Ming Ju, Zijian Zhou, Cong Mu, Xuecai Zhang, Jingyang Gao, Yakun Liang, Jiafa Chen, Yabin Wu, Xiaopeng Li, Shiwei Wang, Jingjing Wen, Luming Yang, Jianyu Wu
Fusarium verticillioides can be transmitted via seeds and cause systemic infection in maize (Zea mays L.); its mycotoxin has harmful effects on animal and human health. We combined QTL mapping in recombinant inbred line (RIL) populations with a genome-wide association study (GWAS) of 217 diverse maize lines using 224,152 single nucleotide polymorphisms (SNPs) under controlled conditions to determine the genetic architecture of F. verticillioides seed rot (FSR) resistance. Our study identified 8 quantitative trait loci (QTLs) and 43 genes associated with 57 SNPs that were correlated with FSR resistance through linkage mapping and GWAS, respectively...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28414630/infection-courts-in-watermelon-plants-leading-to-seed-infestation-by-fusarium-oxysporum-f-sp-niveum
#2
Aparna Petkar, Pingsheng Ji
Fusarium wilt incited by Fusarium oxysporum f. sp. niveum (FON) is a seed-transmitted disease that causes significant yield loss in watermelon production. The pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. However, information regarding infection courts of FON leading to infestation of watermelon seeds is limited. To determine how seeds in watermelon fruit can be infested by FON during the watermelon growing season, greenhouse and field experiments were conducted in 2014 and 2015 where watermelon flowers and immature fruit were inoculated with FON...
April 17, 2017: Phytopathology
https://www.readbyqxmd.com/read/28408912/potential-of-native-rhizobia-in-enhancing-nitrogen-fixation-and-yields-of-climbing-beans-phaseolus-vulgaris-l-in-contrasting-environments-of-eastern-kenya
#3
Gilbert Koskey, Simon W Mburu, Ezekiel M Njeru, Jacinta M Kimiti, Omwoyo Ombori, John M Maingi
Climbing bean (Phaseolus vulgaris L.) production in Kenya is greatly undermined by low soil fertility, especially in agriculturally prolific areas. The use of effective native rhizobia inoculants to promote nitrogen fixation could be beneficial in climbing bean production. In this study, we carried out greenhouse and field experiments to evaluate symbiotic efficiency, compare the effect of native rhizobia and commercial inoculant on nodulation, growth and yield parameters of mid-altitude climbing bean (MAC 13 and MAC 64) varieties...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28408208/establishing-alighting-preferences-and-species-transmission-differences-for-pea-seed-borne-mosaic-virus-aphid-vectors
#4
B S Congdon, B A Coutts, M Renton, G R Flematti, R A C Jones
Pea seed-borne mosaic virus (PSbMV) infection causes a serious disease of field pea (Pisum sativum) crops worldwide. The PSbMV transmission efficiencies of five aphid species previously found landing in south-west Australian pea crops in which PSbMV was spreading were studied. With plants of susceptible pea cv. Kaspa, the transmission efficiencies of Aphis craccivora, Myzus persicae, Acyrthosiphon kondoi and Rhopalosiphum padi were 27%, 26%, 6% and 3%, respectively. Lipaphis erysimi did not transmit PSbMV in these experiments...
April 10, 2017: Virus Research
https://www.readbyqxmd.com/read/28400854/a-rapid-and-efficient-method-for-uniform-gene-expression-using-the-barley-stripe-mosaic-virus
#5
Arnaud Cheuk, Mario Houde
BACKGROUND: The barley stripe mosaic virus (BSMV) has become a popular vector to study gene function in cereals. However, studies have been limited to gene silencing in leaves of barley or wheat. In addition, the method produces high variability between different leaves and plants. To overcome these limitations, we explored the potential of modifying the inoculation protocol for BSMV gene overexpression. An improved light, oxygen or voltage-sensing (iLOV) domain-based fluorescent protein was used as a reporter of gene expression to monitor the infection and spread of BSMV...
2017: Plant Methods
https://www.readbyqxmd.com/read/28397062/multi-metal-resistance-and-plant-growth-promotion-potential-of-a-wastewater-bacterium-pseudomonas-aeruginosa-and-its-synergistic-benefits
#6
Jayanta Kumar Biswas, Monojit Mondal, Jörg Rinklebe, Santosh Kumar Sarkar, Punarbasu Chaudhuri, Mahendra Rai, Sabry M Shaheen, Hocheol Song, Muhammad Rizwan
Water and soil pollution by toxic heavy metals (HMs) is increasing globally because of increase in population, industrialization and urbanization. It is a burning problem for the public, scientists, academicians and politicians how to tackle the toxic contaminants which jeopardize the environment. One possible solution for pollution abatement is a bioremediation-effective and innovative technology that uses biological systems for treatment of contaminants. Many bacteria synthesize indole-3-acetic acid (IAA) which is a product of L-tryptophan metabolism and belongs to the auxin class of plant growth-promoting hormone...
April 10, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28382622/the-plant-growth-promoting-bacteria-kosakonia-radicincitans-improves-fruit-yield-and-quality-of-solanum-lycopersicum
#7
Beatrice Berger, Susanne Baldermann, Silke Ruppel
BACKGROUND: Production and the quality of tomato fruits have a strong economic relevance. Microorganisms such as the plant growth-promoting bacteria Kosakonia radicincitans (DSM 16656) were demonstrated to improve shoot and root growth of young tomato plants, but data on yield increase and fruit quality by K. radicincitans are lacking. RESULTS: Here, we investigated how K. radicincitans affects tomato fruits. After inoculating tomato seeds with K. radicincitans or a sodium chloride buffer control solution, we monitored stalk length, first flowering and the amount of ripened fruits produced by inoculated and non-inoculated plants over a period of 21 weeks...
April 6, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28377343/-three-dimensional-morphology-of-c6-36-cells-infected-by-dengue-virus-a-study-based-on-digital-holographic-microscopy
#8
Jian-Hai Yu, Xu-Ling Liu, Yu-Jing Liu, Xiao-En He, Yuan Hui, Bao Zhang, Li Zhu, Wei Zhao
OBJECTIVE: To monitor the 3-dimensional (3D) morphological changes of C6/36 cells during dengue virus (DENV) infection using a live-cell imaging technique based on digital holographic microscopy and provide clues for better understanding the mechanisms of DENV infection. METHODS: C6/36 cells were seeded in 6-well plates to determine the optimal imaging density under a holographic cell imager, and the morphological changes of the cells were recorded in response to a culture temperature change from 28 degrees celsius; to 37 degrees celsius; C6/36 cells were infected with 4 DENV strains with different serotypes at 28 degrees celsius; and incubated at 37 degrees celsius; for 24 h, and the 3D holograms and relevant morphological parameters were recorded at different time points using HoloMonitor M4 holographic cell imaging and analysis system...
March 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28369915/fruit-quality-of-seedless-watermelon-grafted-onto-squash-rootstocks-under-different-production-systems
#9
Qianru Liu, Xin Zhao, Jeffrey K Brecht, Charles A Sims, Tatiana Sanchez, Nicholas S Dufault
BACKGROUND: The market demand for seedless watermelon has been continuously increasing because of consumer preference. Grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. Currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. This multi-season study was conducted to assess the effects of squash rootstocks, including both Cucurbita maxima × C...
March 29, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28364646/the-influences-of-cr-tolerant-rhizobacteria-in-phytoremediation-and-attenuation-of-cr-vi-stress-in-agronomic-sunflower-helianthus-annuus-l
#10
Ali Bahadur, Rizwan Ahmad, Aftab Afzal, Huyuan Feng, Vishandas Suthar, Asfa Batool, Aman Khan, Muhammad Mahmood-Ul-Hassan
Chromium contamination of agronomic soil has to turn into a serious global problem. This research was pointed to assess the effects of three Cr-tolerant rhizobacteria (SS1, SS3, and SS6) on sunflower growth and heavy metal uptake under Cr smog i.e. 20, 30 and 40 ppm using K2Cr2O7. Root promotion assay and pot experiment were conducted to investigate and evaluate the effects of Cr tolerance rhizobacteria and Cr accumulation capacity of sunflower. From root promotion assay non-significant variation was observed in the root length between SS1 and SS3 compared with un-inoculated whereas SS6 enhanced the root length in the absence and presence of chromium...
March 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/28362984/multivariate-statistics-applied-to-the-reaction-of-common-bean-plants-to-parasitism-by-meloidogyne-javanica
#11
L N S Santos, P D S Cabral, G A R Neves, F R Alves, M B Teixeira, F N Cunha, N F Silva
The availability of common bean cultivars tolerant to Meloidogyne javanica is limited in Brazil. Thus, the present study aimed to evaluate the reactions of 33 common bean genotypes (23 landrace, 8 commercial, 1 susceptible standard and 1 resistant standard) to M. javanica, employing multivariate statistics to discriminate the reaction of the genotypes. The experiment was conducted in a greenhouse using a completely randomized design with seven replicates. The seeds were sown in 1-L pots containing autoclaved soil and sand in a 1:1 ratio (v:v)...
March 16, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28355274/prpsc-formation-and-clearance-as-determinants-of-prion-tropism
#12
Ronald A Shikiya, Katie A Langenfeld, Thomas E Eckland, Jonathan Trinh, Sara A M Holec, Candace K Mathiason, Anthony E Kincaid, Jason C Bartz
Prion strains are characterized by strain-specific differences in neuropathology but can also differ in incubation period, clinical disease, host-range and tissue tropism. The hyper (HY) and drowsy (DY) strains of hamster-adapted transmissible mink encephalopathy (TME) differ in tissue tropism and susceptibility to infection by extraneural routes of infection. Notably, DY TME is not detected in the secondary lymphoreticular system (LRS) tissues of infected hosts regardless of the route of inoculation. We found that similar to the lymphotropic strain HY TME, DY TME crosses mucosal epithelia, enters draining lymphatic vessels in underlying laminae propriae, and is transported to LRS tissues...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28352565/expression-analysis-of-chitinase-upon-challenge-inoculation-to-alternaria-wounding-and-defense-inducers-in-brassica-juncea
#13
Sandhya Rawat, Sajad Ali, Bhabatosh Mittra, Anita Grover
Chitinases are the hydrolytic enzymes which belong to the pathogenesis-related (PR) protein family and play an important role not only in plant defense but also in various abiotic stresses. However, only a limited number of chitinase genes have been characterised in B. juncea. In this study, we have characterised B. juncea class IV chitinase gene (accession no EF586206) in response to fungal infection, salicylic acid (SA), jasmonic acid (JA) treatments and wounding. Gene expression studies revealed that the transcript levels of Bjchitinase (BjChp) gene increases significantly both in local and distal tissues after Alternaria infection...
March 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28352300/toward-facilitating-microalgae-cope-with-effluent-from-anaerobic-digestion-of-kitchen-waste-the-art-of-agricultural-phytohormones
#14
Haiyan Pei, Liqun Jiang, Qingjie Hou, Ze Yu
BACKGROUND: Although numerous studies have used wastewater as substitutes to cultivate microalgae, most of them obtained weaker algal viability than standard media. Some studies demonstrated a promotion of phytohormones on algal growth in standard media. For exploiting a strategy to improve algal biomass accumulation in effluent from anaerobic digestion of kitchen waste (ADE-KW), the agricultural phytohormones gibberellin, indole-3-acetic acid, and brassinolide (GIB) were applied to Chlorella SDEC-11 and Scenedesmus SDEC-13 at different stages of algal growth...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28349199/amyloid-%C3%AE-accumulation-in-the-cns-in-human-growth-hormone-recipients-in-the-uk
#15
Diane L Ritchie, Peter Adlard, Alexander H Peden, Suzanne Lowrie, Margaret Le Grice, Kimberley Burns, Rosemary J Jackson, Helen Yull, Michael J Keogh, Wei Wei, Patrick F Chinnery, Mark W Head, James W Ironside
Human-to-human transmission of Creutzfeldt-Jakob disease (CJD) has occurred through medical procedures resulting in iatrogenic CJD (iCJD). One of the commonest causes of iCJD was the use of human pituitary-derived growth hormone (hGH) to treat primary or secondary growth hormone deficiency. As part of a comprehensive tissue-based analysis of the largest cohort yet collected (35 cases) of UK hGH-iCJD cases, we describe the clinicopathological phenotype of hGH-iCJD in the UK. In the 33/35 hGH-iCJD cases with sufficient paraffin-embedded tissue for full pathological examination, we report the accumulation of the amyloid beta (Aβ) protein associated with Alzheimer's disease (AD) in the brains and cerebral blood vessels in 18/33 hGH-iCJD patients and for the first time in 5/12 hGH recipients who died from causes other than CJD...
March 27, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28322134/reproductive-microbiomes
#16
Michael L Power, Caroline Quaglieri, Jay Schulkin
Almost every part of our body has a coevolved microbial community. The expressed microbial genes comprise the various microbiomes that play important roles in normal physiology and development. The various microbiomes are separate, yet often connected, with the species composition of one affecting others. The female reproductive system microbiomes (eg, vaginal, placental, and mammary/milk) remain less well explored than the gut microbiome although they comprise a large proportion of the female microbial network...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28321368/plant-growth-promoting-endophytic-bacteria-versus-pathogenic-infections-an-example-of-bacillus-amyloliquefaciens-rwl-1-and-fusarium-oxysporum-f-sp-lycopersici-in-tomato
#17
Raheem Shahzad, Abdul Latif Khan, Saqib Bilal, Sajjad Asaf, In-Jung Lee
Fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. Currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. Here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic Bacillus amyloliquefaciens RWL-1 against pathogenic Fusarium oxysporum f. sp. lycopersici. The results revealed significant suppression of pathogenic fungal growth by Bacillus amyloliquefaciens in vitro...
2017: PeerJ
https://www.readbyqxmd.com/read/28289566/differential-attraction-of-drosophilids-to-banana-baits-inoculated-with-saccharomyces-cerevisiae-and-hanseniaspora-uvarum-within-a-neotropical-forest-remnant
#18
Marcos R D Batista, Fabiana Uno, Rafael D Chaves, Rosana Tidon, Carlos A Rosa, Louis B Klaczko
BACKGROUND: Yeasts are a necessary requisite in the diet of most Drosophila species that, in turn, may vector their dispersal in natural environments. Differential attractiveness experiments and the isolation of yeasts consumed by Drosophila may be informative for characterizing this association. Hanseniaspora uvarum is among the most common yeast species isolated from Drosophila crops, with high attractiveness to drosophilids. Saccharomyces cerevisiae has been widely used to collect flies, and it allows broad sampling of almost all local Drosophila species...
2017: PeerJ
https://www.readbyqxmd.com/read/28285373/rhizobia-a-potential-biocontrol-agent-for-soilborne-fungal-pathogens
#19
REVIEW
Krishnashis Das, Radha Prasanna, Anil Kumar Saxena
Rhizobia are a group of organisms that are well known for their ability to colonize root surfaces and form symbiotic associations with legume plants. They not only play a major role in biological nitrogen fixation but also improve plant growth and reduce disease incidence in various crops. Rhizobia are known to control the growth of many soilborne plant pathogenic fungi belonging to different genera like Fusarium, Rhizoctonia, Sclerotium, and Macrophomina. Antagonistic activity of rhizobia is mainly attributed to production of antibiotics, hydrocyanic acid (HCN), mycolytic enzymes, and siderophore under iron limiting conditions...
March 12, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28281925/infectious-prions-and-proteinopathies
#20
Rona M Barron
Transmissible spongiform encephalopathies (TSEs) are caused by an infectious agent that is thought to consist of only misfolded and aggregated prion protein (PrP). Unlike conventional micro-organisms, the agent spreads and propagates by binding to and converting normal host PrP into the abnormal conformer, increasing the infectious titre. Synthetic prions, composed of refolded fibrillar forms of recombinant PrP (rec-PrP) have been generated to address whether PrP aggregates alone are indeed infectious prions...
January 2, 2017: Prion
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