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https://www.readbyqxmd.com/read/29779013/treatment-of-sec62-over-expressing-tumors-by-thapsigargin-and-trifluoperazine
#1
Christina Körbel, Maximilian Linxweiler, Florian Bochen, Silke Wemmert, Bernhard Schick, Markus Meyer, Hans Maurer, Michael D Menger, Richard Zimmermann, Markus Greiner
Treatment with analogues of the SERCA-inhibitor Thapsigargin is a promising new approach for a wide variety of cancer entities. However, our previous studies on various tumor cells suggested resistance of SEC62 over-expressing tumors to this treatment. Therefore, we proposed the novel concept that e.g. lung-, prostate-, and thyroid-cancer patients should be tested for SEC62 over-expression, and developed a novel therapeutic strategy for a combinatorial treatment of SEC62 over-expressing tumors. The latter was based on the observations that treatment of SEC62 over-expressing tumor cells with SEC62-targeting siRNAs showed less resistance to Thapsigargin as well as a reduction in migratory potential and that the siRNA effects can be mimicked by the Calmodulin antagonist Trifluoperazine...
May 19, 2018: Biomolecular Concepts
https://www.readbyqxmd.com/read/29775125/influence-of-tetramethylammonium-hydroxide-tmah-on-the-microbial-properties-of-anaerobic-granular-sludge-acclimated-to-isoplophyl-alcohol-ipa-wastewater-under-psychrophilic-conditions
#2
Tsuyoshi Danshita, Yuma Miyaoka, Norihisa Matsuura, Haruhiko Sumino, Takashi Yamaguchi, Kazuaki Syutsubo
In this study, a continuous flow experiment was conducted in which a lab-scale upflow anaerobic sludge blanket (UASB) reactor at psychrophilic conditions (18-19°C) was fed with artificial wastewater, containing tetramethylammonium hydroxide (TMAH) and isoplophyl alcohol (IPA), from the electronics industry. This was done to evaluate process performance and microbial properties of the granular sludge that was retained in the reactor. The inoculated granular sludge was precultured with IPA containing wastewater but not TMAH; as a result, no degradation was observed in 30 days of operation...
May 18, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29768658/detrimental-effects-of-rhizobial-inoculum-early-in-the-life-of-partridge-pea-chamaecrista-fasciculata
#3
Rachel E Pain, Ruth G Shaw, Seema N Sheth
PREMISE OF THE STUDY: Mutualistic relationships with microbes may aid plants in overcoming environmental stressors and increase the range of abiotic environments where plants can persist. Rhizobia, nitrogen-fixing bacteria associated with legumes, often confer fitness benefits to their host plants by increasing access to nitrogen in nitrogen-limited soils, but effects of rhizobia on host fitness under other stresses, such as drought, remain unclear. METHODS: In this greenhouse study, we varied the application of rhizobia (Bradyrhizobium sp...
May 16, 2018: American Journal of Botany
https://www.readbyqxmd.com/read/29762883/associative-bacteria-influence-maize-zea-mays-l-growth-physiology-and-root-anatomy-under-different-nitrogen-levels
#4
Anderson Kikuchi Calzavara, Pedro Henrique Godoy Paiva, Lorrant Cavanha Gabriel, André Luiz Martinez de Oliveira, Karina Milani, Halley Caixeta Oliveira, Edmilson Bianchini, José Antonio Pimenta, Maria Cristina Neves de Oliveira, Jaqueline Dias-Pereira, Renata Stolf-Moreira
Despite the great diversity of plant growth-promoting bacteria (PGPB) with potential to partially replace the use of N-fertilizers in agriculture, few PGPB are explored for the production of commercial inoculants, reinforcing the importance of identifying positive plant-bacteria interactions. Aiming to better understand the influence of PGPB inoculation in plant development, two PGPB species with distant phylogenetic relationship were inoculated in maize. Maize seeds were inoculated with Bacillus sp. or Azospirillum brasilense...
May 15, 2018: Plant Biology
https://www.readbyqxmd.com/read/29743467/-inhibition-of-growth-of-seed-borne-fungi-and-aflatoxin-production-on-stored-peanuts-by-allyl-isothiocyanate-vapor
#5
Kiyoshi Okano, Chikako Nishioka, Tetsuya Iida, Yuzi Ozu, Misao Kaneko, Yuko Watanabe, Yuichi Mizukami, Masakatsu Ichinoe
Aspergillus parasiticus contamination of peanuts results in the production of highly toxic metabolites, such as aflatoxin B1 , B2 , G1 and G2 , and its incidence in imported peanuts is reported to be increasing. Here, we examined whether the antifungal compound allyl isothiocyanate (AIT), which is present in mustard seed, could inhibit the growth of seed-borne fungi and aflatoxin-producing fungi. Peanuts produced in China and Japan were inoculated with A. parasiticus and exposed to AIT vapor released by a commercial mustard seed extract in closed containers under controlled conditions of temperature and humidity...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/29739645/plant-growth-promoting-rhizobacteria-reduce-aphid-population-and-enhance-the-productivity-of-bread-wheat
#6
Muhammad Naeem, Zubair Aslam, Abdul Khaliq, Jam Nazir Ahmed, Ahmad Nawaz, Mubshar Hussain
Plant growth promoting rhizobacteria increase plant growth and give protection against insect pests and pathogens. Due to the negative impact of chemical pesticides on environment, alternatives to these chemicals are needed. In this scenario, the biological methods of pest control offer an eco-friendly and an attractive option. In this study, the effect of two plant growth promoting rhizobacterial strains (Bacillus sp. strain 6 and Pseudomonas sp. strain 6K) on aphid population and wheat productivity was evaluated in an aphid susceptible (Pasban-90) and resistant (Inqlab-91) wheat cultivar...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29719942/rhizobial-biogeography-and-inoculation-application-to-soybean-in-four-regions-across-china
#7
Sheng Hui Yang, Wen Hao Chen, En Tao Wang, Wen Feng Chen, Jun Yan, Xiao Zeng Han, Chang Fu Tian, Xin Hua Sui, Raghvendra Pratap Singh, Gao Ming Jiang, Wen Xin Chen
AIMS: The aim of the study was to survey rhizobial biogeography and to inoculate soybean with selected rhizobia in China to enhance symbiotic nitrogen fixation (SNF). METHODS AND RESULTS: Biogeography, genetic diversity and phylogeny of soybean rhizobia was surveyed. Inocula were prepared and applied to soybean. Results showed that Bradyrhizobium elkanii and Ensifer fredii were widely distributed in acid and alkaline soils, respectively. Available iron was detected as the first determinant for distribution of the two rhizobia; and the soybean varieties did not greatly affect the rhizobial compatibility...
May 2, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29719451/establishment-and-evaluation-of-the-vx2-orthotopic-lung-cancer-rabbit-model-a-ultra-minimal-invasive-percutaneous-puncture-inoculation-method
#8
Lijuan Wang, Keke Che, Zhonghong Liu, Xianlong Huang, Shifeng Xiang, Fei Zhu, Yu Yu
The purpose of the present work is to establish an ultra-minimal invasive percutaneous puncture inoculation method for a VX2 orthotopic lung cancer rabbit model with fewer technical difficulties, lower mortality of rabbits, a higher success rate and a shorter operation time, to evaluate the growth, metastasis and apoptosis of tumor by CT scans, necropsy, histological examination, flow cytometry and immunohistochemistry. The average inoculation time was 10-15 min per rabbit. The tumor-bearing rate was 100%. More than 90% of the tumor-bearing rabbits showed local solitary tumor with 2-10 mm diameters after two weeks post-inoculation, and the rate of chest seeding was only 8...
May 2018: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/29707522/early-lymphocyte-loss-and-increased-granulocyte-lymphocyte-ratio-predict-systemic-spread-of-streptococcus-pyogenes-in-a-mouse-model-of-acute-skin-infection
#9
Torsten G Loof, Aaqib Sohail, Mahmoud M Bahgat, Aravind Tallam, Haroon Arshad, Manas K Akmatov, Marina C Pils, Ulrike Heise, Andreas Beineke, Frank Pessler
Background: Group A streptococci may induce lymphopenia, but the value of lymphocyte loss as early biomarkers for systemic spread and severe infection has not been examined systematically. Methods: We evaluated peripheral blood cell indices as biomarkers for severity and spread of infection in a mouse model of Streptococcus pyogenes skin infection, using two isolates of greatly differing virulence. Internal organs were examined histologically. Results: After subcutaneous inoculation, strain AP1 disseminated rapidly to peripheral blood and internal organs, causing frank sepsis...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29705203/biocontrol-of-sclerotinia-sclerotiorum-lib-de-bary-on-common-bean-by-native-lipopeptide-producer-bacillus-strains
#10
Daniela C Sabaté, Carolina Pérez Brandan, Gabriela Petroselli, Rosa Erra-Balsells, M Carina Audisio
Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected...
June 2018: Microbiological Research
https://www.readbyqxmd.com/read/29692549/a-novel-trichoderma-fusant-for-enhancing-nutritional-value-and-defence-activity-in-chickpea
#11
Aradhana Mishra, Chandra Shekhar Nautiyal
In recent years, due to the rise in food consumption, much of the attention has been focused to increase the yield of the agricultural crops which resulted in compromised nutritional quality. Efforts have to be undertaken to enhance the nutritional attributes of legumes, cereals and staple food crops by increasing amino acids and mineral content. In the present study, we evaluated a protoplast fusant ( H. lixii MTCC 5659) for its ability to enhance nutritional value and defence activity in chickpea. Essential amino acids; methionine (9...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29669601/potent-prion-like-behaviors-of-pathogenic-%C3%AE-synuclein-and-evaluation-of-inactivation-methods
#12
Airi Tarutani, Tetsuaki Arai, Shigeo Murayama, Shin-Ichi Hisanaga, Masato Hasegawa
The concept that abnormal protein aggregates show prion-like propagation between cells has been considered to explain the onset and progression of many neurodegenerative diseases. Indeed, both synthetic amyloid-like fibrils and pathogenic proteins extracted from patients' brains induce self-templated amplification and cell-to-cell transmission in vitro and in vivo. However, it is unclear whether exposure to exogenous prion-like proteins can potentially cause these diseases in humans. Here, we investigated in detail the prion-like seeding activities of several kinds of pathogenic α-synuclein (α-syn), including synthetic fibrils and detergent-insoluble fractions extracted from brains of patients with α-synucleinopathies...
April 18, 2018: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/29666936/assessment-of-toxic-impact-of-metals-on-proline-antioxidant-enzymes-and-biological-characteristics-of-pseudomonas-aeruginosa-inoculated-cicer-arietinum-grown-in-chromium-and-nickel-stressed-sandy-clay-loam-soils
#13
Saima Saif, Mohammad Saghir Khan
Considering the heavy metal risk to soil microbiota and agro-ecosystems, the study was designed to determine metal toxicity to bacteria and to find metal tolerant bacteria carrying multifarious plant growth promoting activities and to assess their impact on chickpea cultivated in stressed soils. Metal tolerant strain SFP1 recognized as Pseudomonas aeruginosa employing 16S rRNA gene sequence determination showed maximum tolerance to Cr (400 μg/ml) and Ni (800 μg/ml) and produced variable amounts of indole acetic acid, HCN, NH3 , and ACC deaminase and could solubilize insoluble phosphates even under Cr (VI) and Ni stress...
April 17, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29660236/oxylipins-from-both-pathogen-and-host-antagonize-ja-mediated-defense-via-the-9-lipoxygenase-pathway-in-fusarium-vertcillioides-infection-of-maize
#14
Paola Battilani, Alessandra Lanubile, Valeria Scala, Massimo Reverberi, Rossella Gregori, Claudia Falavigna, Chiara Dall'Asta, Yong-Soon Park, John Bennett, Eli J Borrego, Michael V Kolomiets
Oxylipins are a newly emerging group of signals that serve either defense roles or promote virulence. To identify specific host and fungal genes and oxylipins governing the interactions between maize and Fusarium verticillioides, maize wild-type and lipoxygenase3 (lox3) mutant were inoculated with either F. verticillioides wild-type or linoleate-diol-synthase 1-deleted mutant (ΔFvlds1D). Results showed that lox3 mutants were more resistant to F. verticillioides. The reduced colonization on lox3 was associated with reduced fumonisin production and with a stronger and earlier induction of ZmLOX4, ZmLOX5, and ZmLOX12...
April 16, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29628819/exploring-the-potentiality-of-novel-rhizospheric-bacterial-strains-against-the-rice-blast-fungus-magnaporthe-oryzae
#15
Narayanappa Amruta, M K Prasanna Kumar, M E Puneeth, Gowdiperu Sarika, Hemanth Kumar Kandikattu, K Vishwanath, Sonnappa Narayanaswamy
Rice blast caused by Magnaporthe oryzae is a major disease. In the present study, we aimed to identify and evaluate the novel bacterial isolates from rice rhizosphere for biocontrol of M. oryzae pathogen. Sixty bacterial strains from the rice plant's rhizosphere were tested for their biocontrol activity against M. oryzae under in vitro and in vivo . Among them, B. amyloliquefaciens had significant high activity against the pathogen. The least disease severity and highest germination were recorded in seeds treated with B...
April 2018: Plant Pathology Journal
https://www.readbyqxmd.com/read/29614973/in-vitro-antibacterial-activity-of-bioactive-glass-s53p4-on-multiresistant-pathogens-causing-osteomyelitis-and-prosthetic-joint-infection
#16
Mateus Trinconi Cunha, Maria Aparecida Murça, Stanley Nigro, Giselle Burlamaqui Klautau, Mauro José Costa Salles
BACKGROUND: Conventional local treatment for medullary osteomyelitis (OM) includes insertion of antibiotic-loaded polymethylmethacrylate (PMMA) cement. Nevertheless, PMMA may delivery irregular concentration of antibiotic to surrounding tissue. We aimed to compare the in vitro antibacterial activity of Bioactive Glass (BAG) S53P4, which is a compound showing local antibacterial activity, to that of antibiotic-loaded PMMA against multidrug resistant bacteria from OM isolates. METHODS: We studied convenience samples of multidrug resistant (MDR) microorganisms obtained from patients presenting OM and prosthetic joint infection (PJI)...
April 3, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29579955/improving-microbiological-safety-and-quality-characteristics-of-wheat-and-barley-by-high-voltage-atmospheric-cold-plasma-closed-processing
#17
Agata Los, Dana Ziuzina, Simen Akkermans, Daniela Boehm, Patrick J Cullen, Jan Van Impe, Paula Bourke
Contamination of cereal grains as a key global food resource with insects or microorganisms is a persistent concern for the grain industry due to irreversible damage to quality and safety characteristics and economic losses. Atmospheric cold plasma presents an alternative to conventional grain decontamination methods owing to the high antimicrobial potential of reactive species generated during the treatment, but effects against product specific microflora are required to understand how to optimally develop this approach for grains...
April 2018: Food Research International
https://www.readbyqxmd.com/read/29576941/tobacco-rattle-virus-induced-phytoene-desaturase-pds-and-mg-chelatase-h-subunit-chlh-gene-silencing-in-solanum-pseudocapsicum-l
#18
Hua Xu, Leifeng Xu, Panpan Yang, Yuwei Cao, Yuchao Tang, Guoren He, Suxia Yuan, Jun Ming
Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. The present study constitutes the first application of VIGS in S. pseudocapsicum , which has great ornamental and pharmaceutical value, using tobacco rattle virus (TRV) vectors. Two marker genes, PHYTOENE DESATURASE ( PDS ) and Mg-chelatase H subunit ( ChlH ), were used to test the VIGS system in S. pseudocapsicum . The photobleaching and yellow-leaf phenotypes of the silenced plants were shown to significantly correlate with the down-regulation of endogenous SpPDS and SpChlH , respectively ( P  ≤ 0...
2018: PeerJ
https://www.readbyqxmd.com/read/29572468/impact-of-spatial-organization-on-a-novel-auxotrophic-interaction-among-soil-microbes
#19
Xue Jiang, Christian Zerfaß, Song Feng, Ruth Eichmann, Munehiro Asally, Patrick Schäfer, Orkun S Soyer
A key prerequisite to achieve a deeper understanding of microbial communities and to engineer synthetic ones is to identify the individual metabolic interactions among key species and how these interactions are affected by different environmental factors. Deciphering the physiological basis of species-species and species-environment interactions in spatially organized environments requires reductionist approaches using ecologically and functionally relevant species. To this end, we focus here on a defined system to study the metabolic interactions in a spatial context among the plant-beneficial endophytic fungus Serendipita indica, and the soil-dwelling model bacterium Bacillus subtilis...
March 23, 2018: ISME Journal
https://www.readbyqxmd.com/read/29554556/quantification-and-colonisation-dynamics-of-escherichia-coli-o157-h7-inoculation-of-microgreens-species-and-plant-growth-substrates
#20
Kathryn M Wright, Nicola J Holden
Microgreens are edible plants used in food preparation for their appealing flavours and colours. They are grown beyond the point of harvest of sprouted seeds, and normally include the cotyledons and first true leaves. Their method of production is similar to sprouted seeds, which is known to be favourable for growth of microbial pathogens, although there is little data on the potential of food-borne pathogens such as Shigatoxigenic Escherichia coli (STEC) to colonise these plants. We found colonisation of nine different species of microgreen plants by STEC (isolate Sakai, stx-), with high levels of growth over five days, of approximately 5 orders of magnitude, for plants propagated at 21 °C...
March 8, 2018: International Journal of Food Microbiology
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