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https://www.readbyqxmd.com/read/29351192/seed-endophyte-microbiome-of-crotalaria-pumila-unpeeled-identification-of-plant-beneficial-methylobacteria
#1
Ariadna S Sánchez-López, Isabel Pintelon, Vincent Stevens, Valeria Imperato, Jean-Pierre Timmermans, Carmen González-Chávez, Rogelio Carrillo-González, Jonathan Van Hamme, Jaco Vangronsveld, Sofie Thijs
Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp...
January 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29350274/t-lymphocytes-facilitate-brain-metastasis-of-breast-cancer-by-inducing-guanylate-binding-protein-1-expression
#2
Dana A M Mustafa, Rute M S M Pedrosa, Marcel Smid, Marcel van der Weiden, Vanja de Weerd, Alex L Nigg, Cor Berrevoets, Lona Zeneyedpour, Neibla Priego, Manuel Valiente, Theo M Luider, Reno Debets, John W M Martens, John A Foekens, Anieta M Sieuwerts, Johan M Kros
The discovery of genes and molecular pathways involved in the formation of brain metastasis would direct the development of therapeutic strategies to prevent this deadly complication of cancer. By comparing gene expression profiles of Estrogen Receptor negative (ER-) primary breast tumors between patients who developed metastasis to brain and to organs other than brain, we found that T lymphocytes promote the formation of brain metastases. To functionally test the ability of T cells to promote brain metastasis, we used an in vitro blood-brain barrier (BBB) model...
January 19, 2018: Acta Neuropathologica
https://www.readbyqxmd.com/read/29335919/inter-and-intracellular-colonization-of-arabidopsis-roots-by-endophytic-actinobacteria-and-the-impact-of-plant-hormones-on-their-antimicrobial-activity
#3
Anne van der Meij, Joost Willemse, Martinus A Schneijderberg, René Geurts, Jos M Raaijmakers, Gilles P van Wezel
Many actinobacteria live in close association with eukaryotes such as fungi, insects, animals and plants. Plant-associated actinobacteria display (endo)symbiotic, saprophytic or pathogenic life styles, and can make up a substantial part of the endophytic community. Here, we characterised endophytic actinobacteria isolated from root tissue of Arabidopsis thaliana (Arabidopsis) plants grown in soil from a natural ecosystem. Many of these actinobacteria belong to the family of Streptomycetaceae with Streptomyces olivochromogenes and Streptomyces clavifer as well represented species...
January 15, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29330819/preparation-of-a-synthetic-seed-for-the-common-reed-harboring-an-endophytic-bacterium-promoting-seedling-growth-under-cadmium-stress
#4
Ting Gao, Xianyang Shi
Bacterial seed endophytes can facilitate germination and early plant development. Therefore, the introduction of seed-borne endophytes may improve selected plant characteristics across generations. In this study, regenerated plantlets of common reed (Phragmites australis) were inoculated with activated sludge to obtain a specific functional endophytic bacterium. Denaturing gradient gel electrophoresis demonstrated that abundant endophytic bacteria could be enriched in the roots. A siderophore-producing endophytic bacterium was isolated from the roots and identified as Herbaspirillum frisingense RE3-3 based on 16S rRNA sequences...
January 12, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29327464/the-expression-of-an-exogenous-acc-deaminase-by-the-endophyte-serratia-grimesii-bxf1-promotes-the-early-nodulation-and-growth-of-common-bean
#5
Maria J Tavares, Francisco X Nascimento, Bernard R Glick, Márcio J Rossi
Ethylene acts as an inhibitor of the nodulation process of leguminous plants. However, some bacteria can decrease deleterious ethylene levels by the action of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase which degrades ACC, the ethylene precursor in all higher plants. Co-inoculation of rhizobia with endophytes enhances the rhizobial symbiotic efficiency with legumes, improving both nodulation and nitrogen fixation. However, not much is understood about the mechanisms employed by these endophytic bacteria...
January 11, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29323890/hrmas-nmr-and-mri-spectroscopies-distinguish-metabolome-and-structural-properties-of-maize-seeds-from-plants-treated-with-different-fertilizers-and-arbuscular-mycorrhizal-fungi
#6
Pierluigi Mazzei, Vincenza Cozzolino, Alessandro Piccolo
Both High-Resolution Magic-Angle-Spinning (HRMAS) and Magnetic Resonance Imaging (MRI) NMR spectroscopies were applied here to identify the changes of metabolome, morphology and structural properties induced in seeds (caryopses) of maize plants grown at field level under either mineral or compost fertilization in combination with the inoculation by Arbuscular Mycorrhizal Fungi (AMF). The metabolome of intact caryopses was examined by HRMAS-NMR, while the morphological aspects, endosperm properties and seed water distribution were investigated by MRI...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29305540/direct-from-blood-culture-disk-diffusion-to-determine-antimicrobial-susceptibility-of-gram-negative-bacteria-preliminary-report-from-the-clinical-and-laboratory-standards-institute-methods-development-and-standardization-working-group
#7
Sukantha Chandrasekaran, April Abbott, Shelley Miller, Barb Zimmer, Mel Weinstein, Lauri Thrupp, John Hejna, Lindsey Walker, Tracy Ammann, Thomas Kirn, Robin Patel, Romney M Humphries
The performance of disk diffusion, using broth from positive blood cultures as inoculum ("direct disk diffusion", dDD), was evaluated for a collection of 20 challenge isolates of Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa. Isolates seeded into human blood were inoculated into BACTEC™ Plus Aerobic/F, VersaTREK REDOX 1 and BacT/ALERT FA Plus bottles and incubated on the respective automated blood culture systems. Disk diffusion results were compared to reference disk diffusion. Categorical agreement (CA) of dDD, after removal of random errors due to natural MIC variation, was 87...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29302156/root-environment-is-a-key-determinant-of-fungal-entomopathogen-endophytism-following-seed-treatment-in-the-common-bean-phaseolus-vulgaris
#8
Soroush Parsa, Viviana Ortiz, María I Gómez-Jiménez, Matthew Kramer, Fernando E Vega
The common bean is the most important food legume in the world. We examined the potential of the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae applied as seed treatments for their endophytic establishment in the common bean. Endophytic colonization in sterile sand:peat averaged ca. 40% higher for fungus treatments and ca. six times higher for volunteer fungi (other fungal endophytes naturally occurring in our samples), relative to sterile vermiculite. Colonization by B. bassiana and M...
January 2018: Biological Control: Theory and Applications in Pest Management
https://www.readbyqxmd.com/read/29299845/host-specificity-of-symbiotic-mycorrhizal-fungi-for-enhancing-seed-germination-protocorm-formation-and-seedling-development-of-over-collected-medicinal-orchid-dendrobium-devonianum
#9
Hui Huang, Xiao-Meng Zi, Hua Lin, Jiang-Yun Gao
All orchids maintain an obligate relationship with mycorrhizal symbionts during seed germination. In most cases, germination-enhancing fungi have been isolated from roots of mature plants for conservation and cultivation purposes. To understand the germination biology of Dendrobium devonianum, an over-collected medicinal orchid, the seeds of D. devonianum were inoculated with a fungal strain (FDd1) isolated from naturally occurring protocorms of D. devonianum and two other germination-enhancing fungal strains (FDaI7 and FCb4) from D...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29298127/association-between-loss-of-type-iv-pilus-synthesis-ability-and-phenotypic-variation-in-the-cucurbit-pathogenic-bacterium-acidovorax-citrulli
#10
Tally Rosenberg, Bolaji Babajide Salam, Saul Burdman
Acidovorax citrulli is the causal agent of bacterial fruit blotch of cucurbits. We have shown that functional type IV pili (T4P) are required for full virulence of this bacterium. To identify A. citrulli genes required for T4P activity, we screened a library of about 10,000 transposon mutants of A. citrulli strain M6 for altered T4P-mediated twitching motility. This screen led to the identification of 50 mutants impaired in twitching ability due to transposon insertions into 20 different genes. Representative mutants with disruptions in these genes were further characterized...
January 3, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29280676/testing-for-human-norovirus-and-recovery-of-process-control-in-outbreak-associated-produce-items
#11
Kaoru Hida, Efstathia Papafragkou, Michael Kulka
The development of rapid and sensitive detection methods for human noroviruses (HuNoV) in produce items is critical, especially with the recent rise in outbreaks associated with this food commodity. In this study, 50-g portions of various produce items linked to a norovirus outbreak (celery, cucumber, lettuce, grapes, and radish) were artificially inoculated with murine norovirus (MNV-1) and concentrated either by ultracentrifugation or polyethylene glycol (PEG) precipitation after elution with an alkaline Tris-glycine-beef extract buffer supplemented with pectinase...
December 27, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29274462/performance-of-the-bact-alert-virtuo-microbial-detection-system-for-the-culture-of-sterile-body-fluids-a-prospective-multi-centered-study
#12
Rosemary C She, Marc G Romney, Willson Jang, Tamar Walker, James K Karichu, Sandra S Richter
OBJECTIVES: Continuous monitoring blood culture systems are commonly used for sterile body fluid cultures. In this multi-center study, we evaluated the performance of the new generation BacT/Alert Virtuo system compared to the BacT/Alert 3D and conventional culture for the recovery of microorganisms from sterile body fluids. METHODS: Peritoneal, cerebrospinal, pericardial, pleural, and synovial fluids from adult patients submitted for culture were collected from three different centers...
December 20, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29240970/rumen-bacteria-at-work-bioaugmentation-strategies-to-enhance-biogas-production-from-cow-manure
#13
E Gozde Ozbayram, Çağrı Akyol, Bahar Ince, Canan Karakoç, Orhan Ince
AIMS: To investigate the effects of different bioaugmentation strategies for enhancing the biogas production from cow manure and evaluate microbial community patterns. METHODS AND RESULTS: Co-inoculation with cow rumen fluid and cow rumen-derived enriched microbial consortia were evaluated in anaerobic batch tests at 36 °C and 41 °C. Singular addition of both rumen fluid and enriched bioaugmentation culture had a promising enhancement on methane yields; however, the highest methane yield (311 mL CH4 / g VS at 41 °C) was achieved when the anaerobic seed sludge was co-inoculated together with rumen fluid and enriched bioaugmentation culture...
December 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29238277/the-incidence-of-alternaria-species-associated-with-infected-sesamum-indicum-l-seeds-from-fields-of-the-punjab-pakistan
#14
Brian Gagosh Nayyar, Steve Woodward, Luis A J Mur, Abida Akram, Muhammad Arshad, S M Saqlan Naqvi, Shaista Akhund
Sesame (Sesamum indicum) is an important oil seed crop of Asia. Yields can be negatively impacted by various factors, including disease, particularly those caused by fungi which create problems in both production and storage. Foliar diseases of sesame such as Alternaria leaf blight may cause significant yield losses, with reductions in plant health and seed quality. The work reported here determined the incidence of Alternaria species infecting sesame seeds grown in the Punjab, Pakistan. A total of 428 Alternaria isolates were obtained from 105 seed samples and grouped into 36 distinct taxonomic groups based on growth pattern and morphological characters...
December 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/29212934/influenza-a-virus-reassortment-is-limited-by-anatomical-compartmentalization-following-co-infection-via-distinct-routes
#15
Mathilde Richard, Sander Herfst, Hui Tao, Nathan T Jacobs, Anice C Lowen
Exchange of gene segments through reassortment is a major feature of influenza A virus evolution and frequently contributes to the emergence of novel epidemic, pandemic and zoonotic strains. It has long been evident that viral diversification through reassortment is constrained by genetic incompatibility between divergent parental viruses. In contrast, the role of virus-extrinsic factors in determining the likelihood of reassortment has remained unclear. To evaluate the impact of such factors in the absence of confounding effects of segment mismatch, we previously reported an approach in which reassortment between wild-type (wt) and genetically tagged variant (var) viruses of the same strain is measured...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29207902/analgesics-promote-welfare-and-sustain-tumour-growth-in-orthotopic-4t1-and-b16-mouse-cancer-models
#16
Jennifer Lofgren, Amy L Miller, Claudia Chui Shan Lee, Carla Bradshaw, Paul Flecknell, Johnny Roughan
Murine orthotopic cancer models often require surgery, potentially causing pain or distress. However, analgesics are often withheld because they may alter tumour development. Two orthotopically implanted cancers were investigated in mice pre-treated with meloxicam (10 mg/kg), buprenorphine (0.2 mg/kg) or saline (1 ml/kg). Tumours were imaged and welfare was assessed using body weight, behaviour and nociceptive responses. In study 1, BALB/c mice were inoculated with 4T1 mammary carcinoma or saline during surgery or anaesthesia...
January 1, 2017: Laboratory Animals
https://www.readbyqxmd.com/read/29204150/rhizobium-impacts-on-seed-productivity-quality-and-protection-of-pisum-sativum-upon-disease-stress-caused-by-didymella-pinodes-phenotypic-proteomic-and-metabolomic-traits
#17
Nima Ranjbar Sistani, Hans-Peter Kaul, Getinet Desalegn, Stefanie Wienkoop
In field peas, ascochyta blight is one of the most common fungal diseases caused by Didymella pinodes. Despite the high diversity of pea cultivars, only little resistance has been developed until to date, still leading to significant losses in grain yield. Rhizobia as plant growth promoting endosymbionts are the main partners for establishment of symbiosis with pea plants. The key role of Rhizobium as an effective nitrogen source for legumes seed quality and quantity improvement is in line with sustainable agriculture and food security programs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29186799/whole-genome-re-alignment-facilitates-development-of-specific-molecular-markers-for-races-1-and-4-of-xanthomonas-campestris-pv-campestris-the-cause-of-black-rot-disease-in-brassica-oleracea
#18
(no author information available yet)
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is a seed borne disease of Brassicaceae. Eleven pathogenic races have been identified based on the phenotype interaction pattern of differential brassica cultivars inoculated with different strains. Race 1 and 4 are the two most frequent races found in Brassica oleracea crops. In this study, a PCR molecular diagnostic tool was developed for the identification of Xcc races 1 and 4 of this pathogen. Whole genomic sequences of races 1, 3, 4 and 9 and sequences of three other Xanthomonas pathovars/species (X...
November 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29184069/phosphorylated-exogenous-alpha-synuclein-fibrils-exacerbate-pathology-and-induce-neuronal-dysfunction-in-mice
#19
Mantia Karampetsou, Mustafa T Ardah, Maria Semitekolou, Alexia Polissidis, Martina Samiotaki, Maria Kalomoiri, Nour Majbour, Georgina Xanthou, Omar M A El-Agnaf, Kostas Vekrellis
Approximately 90% of alpha-synuclein (α-Synuclein) deposited in Lewy bodies is phosphorylated at serine 129 suggesting that the accumulation of phosphorylated α-Synuclein is critical in the pathogenesis of Parkinson's disease. However, in vivo experiments addressing the role of phosphorylated α-Synuclein in the progression of Parkinson's disease have produced equivocal data. To clarify a role of Ser129 phosphorylation of α-Synuclein in pathology progression we performed stereotaxic injections targeting the mouse striatum with three fibrilar α-Synuclein types: wt-fibrils, phosphorylated S129 fibrils and, phosphorylation incompetent, S129A fibrils...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29182547/gene-silencing-of-argonaute5-negatively-affects-the-establishment-of-the-legume-rhizobia-symbiosis
#20
María Del Rocio Reyero-Saavedra, Zhenzhen Qiao, María Del Socorro Sánchez-Correa, M Enrique Díaz-Pineda, Jose L Reyes, Alejandra A Covarrubias, Marc Libault, Oswaldo Valdés-López
The establishment of the symbiosis between legumes and nitrogen-fixing rhizobia is finely regulated at the transcriptional, posttranscriptional and posttranslational levels. Argonaute5 (AGO5), a protein involved in RNA silencing, can bind both viral RNAs and microRNAs to control plant-microbe interactions and plant physiology. For instance, AGO5 regulates the systemic resistance of Arabidopsis against Potato Virus X as well as the pigmentation of soybean (Glycine max) seeds. Here, we show that AGO5 is also playing a central role in legume nodulation based on its preferential expression in common bean (Phaseolus vulgaris) and soybean roots and nodules...
November 28, 2017: Genes
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