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https://www.readbyqxmd.com/read/28515729/growth-and-yield-responses-of-cowpea-to-inoculation-and-phosphorus-fertilization-in-different-environments
#1
Stephen Kyei-Boahen, Canon E N Savala, David Chikoye, Robert Abaidoo
Cowpea (Vigna unguiculata) is a major source of dietary protein and essential component of the cropping systems in semi-arid regions of Sub-Saharan Africa. However, yields are very low due to lack of improved cultivars, poor management practices, and limited inputs use. The objectives of this study were to assess the effects of rhizobia inoculant and P on nodulation, N accumulation and yield of two cowpea cultivars in Mozambique. Field study was conducted in three contrasting environments during the 2013/2014 and 2014/2015 seasons using randomized complete block design with four replications and four treatments...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28515718/fungal-diversity-in-field-mold-damaged-soybean-fruits-and-pathogenicity-identification-based-on-high-throughput-rdna-sequencing
#2
Jiang Liu, Jun-Cai Deng, Cai-Qiong Yang, Ni Huang, Xiao-Li Chang, Jing Zhang, Feng Yang, Wei-Guo Liu, Xiao-Chun Wang, Tai-Wen Yong, Jun-Bo Du, Kai Shu, Wen-Yu Yang
Continuous rain and an abnormally wet climate during harvest can easily lead to soybean plants being damaged by field mold (FM), which can reduce seed yield and quality. However, to date, the underlying pathogen and its resistance mechanism have remained unclear. The objective of the present study was to investigate the fungal diversity of various soybean varieties and to identify and confirm the FM pathogenic fungi. A total of 62,382 fungal ITS1 sequences clustered into 164 operational taxonomic units (OTUs) with 97% sequence similarity; 69 taxa were recovered from the samples by internal transcribed spacer (ITS) region sequencing...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28515390/biocontrol-potential-of-an-endophytic-streptomyces-sp-strain-mbcn152-1-against-alternaria-brassicicola-on-cabbage-plug-seedlings
#3
Naglaa Hassan, Satoko Nakasuji, Mohsen Mohamed Elsharkawy, Hushna Ara Naznin, Masaharu Kubota, Hammad Ketta, Masafumi Shimizu
In the present study, 77 strains of endophytic actinomycetes isolated from cabbage were screened in order to assess their biocontrol potential against Alternaria brassicicola on cabbage seedlings. In the first and second screening trials, cabbage seedlings pretreated with mycelial suspensions of each isolate were spray-inoculated with A. brassicicola. Strain MBCN152-1, which exhibited the best protection in screening trials and had no adverse effects on seedling growth, was selected for the greenhouse trial...
May 18, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28515213/rapid-identification-of-five-classes-of-carbapenem-resistance-genes-directly-from-rectal-swabs-using-the-xpert%C3%A2-carba-r-assay
#4
Nicholas M Moore, Rafael Cantón, Edoardo Carretto, Lance R Peterson, Robert L Sautter, Maria M Traczewski
Carbapenemase-producing organisms (CPO) have been identified by global health leaders as an urgent threat. Detection of patients with gastrointestinal carriage of CPO is necessary to interrupt their spread within healthcare facilities. In this multi-site study, we assessed the performance of the Xpert® Carba-R test, a rapid real-time PCR (qPCR) assay that detects five families of carbapenemase genes (blaIMP, blaKPC, blaNDM, blaOXA-48, and blaVIM) directly from rectal swab specimens. Using dual swabs, specimens from 755 patients were collected and tested prospectively...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28510188/mitigation-of-salt-stress-in-white-clover-trifolium-repens-by-azospirillum-brasilense-and-its-inoculation-effect
#5
Muhammad Khalid, Muhammad Bilal, Danial Hassani, Hafiz M N Iqbal, Hang Wang, Danfeng Huang
BACKGROUND: Salinity is one of the increasingly serious environmental problems worldwide for cultivating agricultural crops. The present study was aimed to ascertain the potential of beneficial soil bacterium Azospirillum brasilense to alleviate saline stress in Trifolium repens. Experimental plants (white clover) were grown from seeds and inoculated with or without A. brasilense bacterial strain supplemented with 0, 40, 80, or 120 mM NaCl into soil. RESULTS: The growth attributes including, shoot heights, root lengths, fresh and dry weights, leaf area and chlorophyll content were significantly enhanced in T...
December 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28500506/a-controlled-comparison-of-the-bact-alert%C3%A2-3d-and-virtuo%C3%A2-microbial-detection-systems
#6
H Totty, M Ullery, J Spontak, J Viray, M Adamik, B Katzin, W M Dunne, P Deol
The performance of the next-generation BacT/ALERT® VIRTUO™ Microbial Detection System (VIRTUO™, bioMérieux Inc., Hazelwood, MO) was compared to the BacT/ALERT® 3D Microbial Detection System (3D, bioMérieux Inc., Durham, NC) using BacT/ALERT® FA Plus (FA Plus), BacT/ALERT® PF Plus (PF Plus), BacT/ALERT® FN Plus (FN Plus), BacT/ALERT® Standard Aerobic (SA), and BacT/ALERT® Standard Anaerobic (SN) blood culture bottles (bioMérieux Inc., Durham, NC). A seeded limit of detection (LoD) study was performed for each bottle type in both systems...
May 12, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28472087/beneficial-rhizobacteria-immobilized-in-nanofibers-for-potential-application-as-soybean-seed-bioinoculants
#7
Priscilla Romina De Gregorio, Gabriela Michavila, Lenise Ricciardi Muller, Clarissa de Souza Borges, María Fernanda Pomares, Enilson Luiz Saccol de Sá, Claudio Pereira, Paula Andrea Vincent
Seed inoculation with plant growth promoting rhizobacteria (PGPR) is an ideal tool to supply the soil with a high density of beneficial microorganisms. However, maintaining viable microorganisms is a major problem during seed treatment and storage. In this work, an evaluation was made of the effect of bacterial immobilization in nanofibers on the stability (viability and maintenance of beneficial properties) of two potential PGPR, Pantoea agglomerans ISIB55 and Burkholderia caribensis ISIB40. Moreover, the impact of soybean seed coating with nanofiber-immobilized rhizobacteria on bacterial survival during seed storage and on germination and plant growth parameters was determined...
2017: PloS One
https://www.readbyqxmd.com/read/28463084/fate-of-salmonella-throughout-production-and-refrigerated-storage-of-tahini
#8
Yangjunna Zhang, Susanne E Keller, Elizabeth M Grasso-Kelley
Tahini, a low-moisture food that is made from sesame seeds, has been implicated in outbreaks of salmonellosis. In this study, the fate of Salmonella was determined through an entire process for the manufacture of tahini, including a 24-h seed soaking period before roasting, subsequent grinding, and storage at refrigeration temperature. Salmonella populations increased by more than 3 log CFU/g during a 24-h soaking period, reaching more than 7 log CFU/g. Survival of Salmonella during roasting at three temperatures, 95, 110, and 130°C, was assessed using seeds on which Salmonella was grown...
May 2, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28455133/development-and-application-of-a-quantitative-pcr-assay-to-study-equine-herpesvirus-5-invasion-and-replication-in-equine-tissues-in-vitro-and-in-vivo
#9
Lila M Zarski, Emily A High, Rahul K Nelli, Steven R Bolin, Kurt J Williams, Gisela Soboll Hussey
Equine herpesvirus 5 (EHV-5) infection is associated with pulmonary fibrosis in horses, but further studies on EHV-5 persistence in equine cells are needed to fully understand viral and host contributions to disease pathogenesis. Our aim was to develop a quantitative PCR (qPCR) assay to measure EHV-5 viral copy number in equine cell cultures, blood lymphocytes, and nasal swabs of horses. Furthermore, we used a recently developed equine primary respiratory cell culture system to study EHV-5 pathogenesis at the respiratory tract...
April 25, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28454714/growth-promotion-of-peanut-arachis-hypogaea-l-and-maize-zea-mays-l-plants-by-single-and-mixed-cultures-of-efficient-phosphate-solubilizing-bacteria-that-are-tolerant-to-abiotic-stress-and-pesticides
#10
María Soledad Anzuay, María Gabriela Ruiz Ciancio, Liliana Mercedes Ludueña, Jorge Guillermo Angelini, Germán Barros, Nicolás Pastor, Tania Taurian
The aims of this study were, to analyze in vitro phosphate solubilization activity of six native peanut bacteria and to determine the effect of single and mixed inoculation of these bacteria on peanut and maize plants. Ability to produce organic acids and cofactor PQQ, to solubilize FePO4 and AlPO4 and phosphatase activity were analyzed. Also, the ability to solubilize phosphate under abiotic stress and in the presence of pesticides of the selected bacteria was determined. The effect of single and mixed bacterial inocula was analyzed on seed germination, maize plant growth and in a crop rotation plant assay with peanut and maize...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28448936/composite-of-pah-degrading-endophytic-bacteria-reduces-contamination-and-health-risks-caused-by-pahs-in-vegetables
#11
Jian Wang, Juan Liu, Wanting Ling, Qingguo Huang, Yanzheng Gao
Vegetables accumulate polycyclic aromatic hydrocarbons (PAHs) at high concentrations when grown in contaminated sites. Inoculation with PAH-degrading endophytic bacteria (EBPAH) has been recognized as one of the most promising ways to remove PAHs from plant bodies; however, the performance of single endophytic bacteria is generally limited. This investigation used a composite of eight EBPAH to reduce the contamination and health risk posed by 16 EPA priority PAHs in vegetables including Chinese cabbage (Brassica chinensis L...
April 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28447887/cyclopiazonic-acid-is-a-pathogenicity-factor-for-aspergillus-flavus-and-a-promising-target-for-screening-germplasm-for-ear-rot-resistance
#12
Subbaiah C Chalivendra, Catherine DeRobertis, Perng-Kuang Chang, Kenneth E Damann
Aspergillus flavus, an opportunistic pathogen, contaminates maize and other key crops with carcinogenic aflatoxins (AFs). Besides AFs, A. flavus makes many more secondary metabolites (SMs) whose toxicity in insects or vertebrates has been studied. However, the role of SMs in the invasion of plant hosts by A. flavus remains to be investigated. Cyclopiazonic acid (CPA), a neurotoxic SM made by A. flavus, is a nanomolar inhibitor of endoplasmic reticulum calcium ATPases (ECAs) and a potent inducer of cell death in plants...
May 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28444566/improvement-of-rice-seedling-growth-and-nitrogen-use-efficiency-by-seed-inoculation-with-endophytic-denitrifiers
#13
Wenfeng Wang, Yanyan Zhai, Lixiang Cao, Hongming Tan, Renduo Zhang
The aim of the present study is to investigate the effect of seed inoculation with endophytic denitrifiers on rice seedling growth and nitrogen use efficiency under low- and high-urea conditions. Pseudomonas sp. B2, Streptomyces sp. A9, and Fusarium sp. F3 were isolated from rice plant tissues. Rice seeds inoculated with the denitrifiers were sown in soil fertilized with 100 and 300 mg/kg urea concentrations, respectively. The denitrifiers increased soil ammonia concentrations or kept high ammonia concentration for a longer time in soils...
April 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28436040/synergistic-use-of-biochar-compost-and-plant-growth-promoting-rhizobacteria-for-enhancing-cucumber-growth-under-water-deficit-conditions
#14
Sajid M Nadeem, Muhammad Imran, Muhammad Naveed, Muhammad Y Khan, Maqshoof Ahmad, Zahir A Zahir, David E Crowley
BACKGROUND: Limited information is available about effectiveness of biochar with plant growth promoting rhizobacteria (PGPR) and compost. A greenhouse study was conducted to evaluate the effect of biochar in combination with compost and PGPR (Pseudomonas fluorescens) for alleviating water deficit stress. The inoculated and un-inoculated cucumber seeds were sown in soil treated with biochar, compost and biochar + compost. Three water levels [field capacity (D0), 75% field capacity (D1) and 50% field capacity (D2)] were maintained...
April 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28422143/dissecting-the-genetic-architecture-of-fusarium-verticillioides-seed-rot-resistance-in-maize-by-combining-qtl-mapping-and-genome-wide-association-analysis
#15
Ming Ju, Zijian Zhou, Cong Mu, Xuecai Zhang, Jingyang Gao, Yakun Liang, Jiafa Chen, Yabin Wu, Xiaopeng Li, Shiwei Wang, Jingjing Wen, Luming Yang, Jianyu Wu
Fusarium verticillioides can be transmitted via seeds and cause systemic infection in maize (Zea mays L.); its mycotoxin has harmful effects on animal and human health. We combined QTL mapping in recombinant inbred line (RIL) populations with a genome-wide association study (GWAS) of 217 diverse maize lines using 224,152 single nucleotide polymorphisms (SNPs) under controlled conditions to determine the genetic architecture of F. verticillioides seed rot (FSR) resistance. Our study identified 8 quantitative trait loci (QTLs) and 43 genes associated with 57 SNPs that were correlated with FSR resistance through linkage mapping and GWAS, respectively...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28414630/infection-courts-in-watermelon-plants-leading-to-seed-infestation-by-fusarium-oxysporum-f-sp-niveum
#16
Aparna Petkar, Pingsheng Ji
Fusarium wilt incited by Fusarium oxysporum f. sp. niveum (FON) is a seed-transmitted disease that causes significant yield loss in watermelon production. The pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. However, information regarding infection courts of FON leading to infestation of watermelon seeds is limited. To determine how seeds in watermelon fruit can be infested by FON during the watermelon growing season, greenhouse and field experiments were conducted in 2014 and 2015 where watermelon flowers and immature fruit were inoculated with FON...
April 17, 2017: Phytopathology
https://www.readbyqxmd.com/read/28408912/potential-of-native-rhizobia-in-enhancing-nitrogen-fixation-and-yields-of-climbing-beans-phaseolus-vulgaris-l-in-contrasting-environments-of-eastern-kenya
#17
Gilbert Koskey, Simon W Mburu, Ezekiel M Njeru, Jacinta M Kimiti, Omwoyo Ombori, John M Maingi
Climbing bean (Phaseolus vulgaris L.) production in Kenya is greatly undermined by low soil fertility, especially in agriculturally prolific areas. The use of effective native rhizobia inoculants to promote nitrogen fixation could be beneficial in climbing bean production. In this study, we carried out greenhouse and field experiments to evaluate symbiotic efficiency, compare the effect of native rhizobia and commercial inoculant on nodulation, growth and yield parameters of mid-altitude climbing bean (MAC 13 and MAC 64) varieties...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28408208/establishing-alighting-preferences-and-species-transmission-differences-for-pea-seed-borne-mosaic-virus-aphid-vectors
#18
B S Congdon, B A Coutts, M Renton, G R Flematti, R A C Jones
Pea seed-borne mosaic virus (PSbMV) infection causes a serious disease of field pea (Pisum sativum) crops worldwide. The PSbMV transmission efficiencies of five aphid species previously found landing in south-west Australian pea crops in which PSbMV was spreading were studied. With plants of susceptible pea cv. Kaspa, the transmission efficiencies of Aphis craccivora, Myzus persicae, Acyrthosiphon kondoi and Rhopalosiphum padi were 27%, 26%, 6% and 3%, respectively. Lipaphis erysimi did not transmit PSbMV in these experiments...
April 11, 2017: Virus Research
https://www.readbyqxmd.com/read/28400854/a-rapid-and-efficient-method-for-uniform-gene-expression-using-the-barley-stripe-mosaic-virus
#19
Arnaud Cheuk, Mario Houde
BACKGROUND: The barley stripe mosaic virus (BSMV) has become a popular vector to study gene function in cereals. However, studies have been limited to gene silencing in leaves of barley or wheat. In addition, the method produces high variability between different leaves and plants. To overcome these limitations, we explored the potential of modifying the inoculation protocol for BSMV gene overexpression. An improved light, oxygen or voltage-sensing (iLOV) domain-based fluorescent protein was used as a reporter of gene expression to monitor the infection and spread of BSMV...
2017: Plant Methods
https://www.readbyqxmd.com/read/28397062/multi-metal-resistance-and-plant-growth-promotion-potential-of-a-wastewater-bacterium-pseudomonas-aeruginosa-and-its-synergistic-benefits
#20
Jayanta Kumar Biswas, Monojit Mondal, Jörg Rinklebe, Santosh Kumar Sarkar, Punarbasu Chaudhuri, Mahendra Rai, Sabry M Shaheen, Hocheol Song, Muhammad Rizwan
Water and soil pollution by toxic heavy metals (HMs) is increasing globally because of increase in population, industrialization and urbanization. It is a burning problem for the public, scientists, academicians and politicians how to tackle the toxic contaminants which jeopardize the environment. One possible solution for pollution abatement is a bioremediation-effective and innovative technology that uses biological systems for treatment of contaminants. Many bacteria synthesize indole-3-acetic acid (IAA) which is a product of L-tryptophan metabolism and belongs to the auxin class of plant growth-promoting hormone...
April 10, 2017: Environmental Geochemistry and Health
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