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https://www.readbyqxmd.com/read/28181101/preparation-of-hydroxyapatite-collagen-injectable-bone-paste-with-an-anti-washout-property-utilizing-sodium-alginate-part-1-influences-of-excess-supplementation-of-calcium-compounds
#1
Taira Sato, Masanori Kikuchi, Mamoru Aizawa
The anti-washout property, viscosity, and cytocompatibility to an osteoblastic cell line, MG-63, of anti-washout pastes were investigated. Mixing a hydroxyapatite/collagen bone-like nanocomposite (HAp/Col), an aqueous solution of sodium alginate (Na-Alg), which is a paste hardening and lubricant agent, and supplementation of calcium carbonate or calcium citrate (Ca-Cit) as a calcium resource for the hardening reaction realized an injectable bone paste. Adding Ca-Cit at a concentration greater than eight times the Ca(2+) ion concentration to Na-Alg improved the anti-washout property...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28170076/biomimetic-sulphated-alginate-hydrogels-suppress-il-1%C3%AE-induced-inflammatory-responses-in-human-chondrocytes
#2
Ø Arlov, M Steinwachs, G Skjåk-Bræk, M Zenobi-Wong
Loss of articular cartilage from ageing, injury or degenerative disease is commonly associated with inflammation, causing pain and accelerating degradation of the cartilage matrix. Sulphated glycosaminoglycans (GAGs) are involved in the regulation of immune responses in vivo, and analogous polysaccharides are currently being evaluated for tissue engineering matrices to form a biomimetic environment promoting tissue growth while suppressing inflammatory and catabolic activities. Here, we characterise physical properties of sulphated alginate (S-Alg) gels for use in cartilage engineering scaffolds, and study their anti-inflammatory effects on encapsulated chondrocytes stimulated with IL-1β...
February 7, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28169241/enhanced-cytotoxic-activity-of-endophytic-bacterial-extracts-from-adhatoda-beddomei-leaves-in-a549-lung-cancer-cell-lines
#3
Y Swarnalatha, Bhaswti Saha
AIM OF THE STUDY: The current study is aimed to isolate and study the efficacy of the anticancer activity of endophytic bacteria from adhatoda beddomei leaves. Endophytic bacteria, microorganisms can found in the plant tissues, like leaves, branches and roots and able to produce various novel secondary metabolites for the medicinal applications. METHODOLOGY: Endophytic bacteria were isolated from the leaves of the adhatoda beddomei leaves. The extract from the culture was tested for the cytotoxicity in A549 cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay, dual staining and nuclear staining...
October 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28161663/is-there-a-cause-and-effect-relationship-between-physicochemical-properties-and-cell-behavior-of-alginate-based-hydrogel-obtained-after-sterilization
#4
Hao Yu, Ghislaine Cauchois, Jean-François Schmitt, Nicolas Louvet, Jean-Luc Six, Yun Chen, Rachid Rahouadj, Céline Huselstein
Alginate-based hydrogel scaffolds are widely used in the field of cartilage regeneration and repair. If the effect of autoclaving on the alginate powder is well known, it is not the same for the possible effects of the sterilization UV treatment on the properties of the hydrogel after polymerization. To select an effective sterilization treatment of alginate-based materials, one must find what are inter-relationship between the characteristics (chemical, physical and mechanical) of alginate-based hydrogel during sterilization, and what consequences have affected on cell behavior...
January 25, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28153040/bicpams-software-for-biological-data-analysis-with-pattern-based-biclustering
#5
Rui Henriques, Francisco L Ferreira, Sara C Madeira
BACKGROUND: Biclustering has been largely applied for the unsupervised analysis of biological data, being recognised today as a key technique to discover putative modules in both expression data (subsets of genes correlated in subsets of conditions) and network data (groups of coherently interconnected biological entities). However, given its computational complexity, only recent breakthroughs on pattern-based biclustering enabled efficient searches without the restrictions that state-of-the-art biclustering algorithms place on the structure and homogeneity of biclusters...
February 2, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28150705/bioengineering-a-bacterial-pathogen-to-assemble-its-own-particulate-vaccine-capable-of-inducing-cellular-immunity
#6
Jason W Lee, Natalie A Parlane, D Neil Wedlock, Bernd H A Rehm
Many bacterial pathogens naturally form cellular inclusions. Here the immunogenicity of polyhydroxyalkanoate (PHA) inclusions and their use as particulate vaccines delivering a range of host derived antigens was assessed. Our study showed that PHA inclusions of pathogenic Pseudomonas aeruginosa are immunogenic mediating a specific cell-mediated immune response. Protein engineering of the PHA inclusion forming enzyme by translational fusion of epitopes from vaccine candidates outer membrane proteins OprI, OprF, and AlgE mediated self-assembly of PHA inclusions coated by these selected antigens...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28137463/validated-hplc-method-for-the-pharmacokinetic-study-of-oral-extended-release-cefpodoxime-proxetil-chitosan-alginate-beads-in-rabbits
#7
Ali Mujtaba, Kanchan Kohli
The aim of this study is to develop a simple and applicable HPLC method for the detection of cefpodoxime acid (CFA) in rabbit plasma after oral administration of cefpodoxime proxetil (CFP) loaded chitosan-alginate (CH-ALG) beads formulation. CFP is a prodrug that is deesterified in vivo to its active metabolite CFA to exhibit antibiotic activity. Chromatographic separation of CFA and internal standard (IS) was achieved by a RP18(C18), Phenomenax®100, (250×4.6mm) with the mobile phase consisting of (0.02mol/l (20mM) ammonium acetate solution and acetonitrile (92:8, v/v, pH=4...
January 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28134392/chitosan-based-water-propelled-micromotors-with-strong-antibacterial-activity
#8
Jorge A M Delezuk, Doris E Ramírez-Herrera, Berta Esteban-Fernández de Ávila, Joseph Wang
A rapid and efficient micromotor-based bacteria killing strategy is described. The new antibacterial approach couples the attractive antibacterial properties of chitosan with the efficient water-powered propulsion of magnesium (Mg) micromotors. These Janus micromotors consist of Mg microparticles coated with the biodegradable and biocompatible polymers poly(lactic-co-glycolic acid) (PLGA), alginate (Alg) and chitosan (Chi), with the latter responsible for the antibacterial properties of the micromotor. The distinct speed and efficiency advantages of the new micromotor-based environmentally friendly antibacterial approach have been demonstrated in various control experiments by treating drinking water contaminated with model Escherichia coli (E...
January 30, 2017: Nanoscale
https://www.readbyqxmd.com/read/28122206/kinetic-and-thermodynamic-properties-of-alginate-lyase-and-cellulase-co-produced-by-exiguobacterium-species-alg-s5
#9
Bidyut R Mohapatra
In an effort to screen out the alginolytic and cellulolytic bacteria from the putrefying invasive seaweed Sargassum species accumulated off Barbados' coast, a potent bacterial strain was isolated. This bacterium, which simultaneously produced alginate lyase and cellulase, was identified as Exiguobacterium sp. Alg-S5 via the phylogenetic approach targeting the 16S rRNA gene. The co-produced alginate lyase and cellulase exhibited maximal enzymatic activity at pH 7.5 and at 40°C and 45°C, respectively. The Km and Vmax values recorded as 0...
January 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28121342/clinical-applications-of-haploidentical-hematopoietic-stem-cell-transplantation-in-severe-aplastic-anemia
#10
P Zhang, K Feng, Y Xue, C-X Zhang, Y Wang, X-L Li
OBJECTIVE: The purpose of this study was to investigate the efficacy and safety of haploidentical allogeneic hemopoietic stem cell transplantation (allo-HSCT) in severe aplastic anemia (SAA) and prophylaxis of complications involved. PATIENTS AND METHODS: 8 patients with clinically diagnosed SAA (5 cases of SAA-I and 3 cases of SAA-II) were recruited, with the parents as the donors of hemopoietic stem cells. The conditioning regimen before HSCT included cyclophosphamide, fludarabine, pig anti-human lymphocyte immune globulin (p-ALG) and/or total body irradiation (TBI)...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28118563/analytical-stability-indicating-methods-for-alogliptin-in-tablets-by-lc-cad-and-lc-uv
#11
Charise Dallazem Bertol, Maria Tereza Friedrich, Graciela Carlos, Pedro Eduardo Froehlich
Stability-indicating LC methods using a UV detector and a charged aerosol detector (CAD) simultaneously were validatedfor the assessment of alogliptin (ALG) in tablets. The analysis was performed on a C<sub>8</sub> column (250 × 4.6 mm, 5 μm) at a flow of 0.8 mL/min, using acetonitrile-10 mM ammonium acetate buffer (pH 3.5; 90 + 10, v/v) as mobile phase and UV detection at 275 nm. Validation followed the International Conference on Harmonization guidelines. The method was linear over the range of 25-200 μg/mL...
December 15, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28115104/a-facile-method-for-preparation-superhydrophobic-paper-with-enhanced-physical-strength-and-moisture-proofing-property
#12
Hui Li, Jin Yang, Pan Li, Tianqing Lan, Lincai Peng
We proposed a green and facile method to fabricate superhydrophobic paper in this study, which is layer-by-layer (LBL) deposition of TiO2 nanoparticles/sodium alginate (ALG) multilayers on paper surface followed by an adsorption treatment of colloidal carnauba wax. The formation of TiO2/ALG multilayers on paper surface was characterized by X-ray photoelectron spectroscopy (XPS), zeta potential measurement, scanning electron microscopy (SEM) and atomic force microscopy (AFM), respectively. The wetting property of modified paper was investigated by water contact angle (WCA) measurement...
March 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28051909/in-vitro-heterogeneous-degradation-of-alginate-and-its-validation-of-different-molecular-weight-on-blood-bio-compatibility
#13
Mengxue Xu, Chao Feng, Juan Wang, Xuqian Lang, Guixue Xia, Xiaoping Yu, Qiuxia Ji, Xiaojie Cheng, Ming Kong, Ya Liu, Xiguang Chen
In the present work, sodium alginate (ALG) was degraded by heterogeneous phase acid degradation. The molecular weight distribution of ALG after degradation was close to homogenization. Then the blood bio-compatibility of ALG with different molecular weights (ALG-0h 50,075, ALG-0.5h 20,680, ALG-2h 13,170 and ALG-96h 1170 kDa) was evaluated in vitro and vivo. The human umbilical vein endothelial cells were used to assess the cytotoxicity of ALGs, ALG-0.5h and ALG-2h exhibited greater increment in percentage of cell viability comparing with ALG-0h and ALG-96h...
January 18, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28035178/harnessing-the-bethe-free-energy
#14
Victor Bapst, Amin Coja-Oghlan
A wide class of problems in combinatorics, computer science and physics can be described along the following lines. There are a large number of variables ranging over a finite domain that interact through constraints that each bind a few variables and either encourage or discourage certain value combinations. Examples include the k-SAT problem or the Ising model. Such models naturally induce a Gibbs measure on the set of assignments, which is characterised by its partition function. The present paper deals with the partition function of problems where the interactions between variables and constraints are induced by a sparse random (hyper)graph...
December 2016: Random Struct Algorithms
https://www.readbyqxmd.com/read/28027587/analysis-of-surgical-interruption-of-the-enterohepatic-circulation-as-a-treatment-for-pediatric-cholestasis
#15
Kasper S Wang, Greg Tiao, Lee M Bass, Paula M Hertel, Douglas Mogul, Nanda Kerkar, Matthew Clifton, Colleen Azen, Laura Bull, Philip Rosenthal, Dylan Stewart, Riccardo Superina, Ronen Arnon, Molly Bozic, Mary L Brandt, Patrick A Dillon, Annie Fecteau, Kishore Iyer, Binita Kamath, Saul Karpen, Frederick Karrer, Kathleen M Loomes, Cara Mack, Peter Mattei, Alexander Miethke, Kyle Soltys, Yumirle P Turmelle, Karen West, Jessica Zagory, Cat Goodhue, Benjamin L Shneider
: To evaluate the efficacy of non-transplant surgery for pediatric cholestasis, 58 clinically diagnosed children, including 20 Alagille syndrome (ALGS), 16 Familial Intrahepatic Cholestasis-1 (FIC1), 18 Bile Salt Export Pump (BSEP) disease, & 4 others with low γ-glutamyl transpeptidase disease (levels <100 U/L), were identified across 14 Childhood Liver Disease Research Network (ChiLDReN) centers. Data were collected retrospectively from individuals who collectively had 39 partial external biliary diversions (PEBD), 11 ileal exclusions (IE) and 7 gallbladder-to-colon diversion (GBC)...
December 27, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28004381/deregulated-alg-2-hebp2-axis-alters-microtubule-dynamics-and-mitotic-spindle-behavior-to-stimulate-cancer-development
#16
Juan Qin, Yang Yang, Siqi Gao, Yang Liu, Fan Yu, Yunqiang Zhou, Rui Lyu, Min Liu, Xinqi Liu, Dengwen Li, Jun Zhou
Cancer cells are characterized by genomic instability, resulting in the accumulation of mutations that promote cancer progression. One way that genomic instability can arise is through improper regulation of the microtubule cytoskeleton that impacts the function of the mitotic spindle. In this study, we have identified a critical role for the interaction between apoptosis-linked gene 2 (ALG-2) and heme-binding protein 2 (HEBP2) in the above processes. Our data show that the gene copy numbers and mRNA levels for both ALG-2 and HEBP2 are significantly upregulated in breast and lung cancer...
December 22, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27995878/-treatment-of-transfusion-dependent-nonsevere-aplastic-anemia-with-cyclosporine-a-plus-atg-alg-versus-cyclosporine-a-plus-androgens-a-retrospective-single-center-study
#17
L Song, G X Peng, Z J Wu, L Zhang, L P Jing, K Zhou, Y Li, Y Li, L Ye, J P Li, H H Fan, X Zhao, W R Yang, Y Yang, F K Zhang
Objective: To determine whether cyclosporine A (CsA) plus androgens was as effective as the current standard immunosuppressive therapy (IST) for transfusion-dependent nonsevere aplastic anemia (TD-NSAA). Methods: The records of 125 consecutive TD-NSAA patients who were treated between Aug. 2007 and Sept. 2014, with either CsA plus androgen or ALG/ATG plus CsA regimen were reviewed. The 3-month and 6-month hematologic responses and survival were evaluated. Results: There were 125 TD-NSAA patients (70 were male and 55 female, 1...
November 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/27990217/flexible-control-of-cellular-encapsulation-permeability-and-release-in-a-droplet-templated-bifunctional-copolymer-scaffold
#18
Qiushui Chen, Dong Chen, Jing Wu, Jin-Ming Lin
Designing cell-compatible, bio-degradable, and stimuli-responsive hydrogels is very important for biomedical applications in cellular delivery and micro-scale tissue engineering. Here, we report achieving flexible control of cellular microencapsulation, permeability, and release by rationally designing a diblock copolymer, alginate-conjugated poly(N-isopropylacrylamide) (Alg-co-PNiPAM). We use the microfluidic technique to fabricate the bifunctional copolymers into thousands of mono-disperse droplet-templated hydrogel microparticles for controlled encapsulation and triggered release of mammalian cells...
November 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27987760/cell-laden-3d-bioprinting-hydrogel-matrix-depending-on-different-compositions-for-soft-tissue-engineering-characterization-and-evaluation
#19
Jisun Park, Sang Jin Lee, Solchan Chung, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Su A Park
Cell-printing techniques that can construct three-dimensional (3D) structures with biocompatible materials and cells are of great interest for various biomedical applications, such as tissue engineering and drug-screening studies. For successful cell-printing with cells, bioinks are critical for both the processability of printing and the viability of printed cells. However, the influence of composition on 3D bio-printing with cells has not been well explored. In this study, we investigated different compositions of alginate bioinks by varying the concentrations of high molecular weight alginate (High Alg) and low molecular weight alginate (Low Alg)...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27986630/molecular-mechanism-of-lysozyme-adsorption-onto-chemically-modified-alginate-guar-gum-matrix
#20
Ma Emilia Brassesco, Nadia Woitovich Valetti, Guillermo Picó
The equilibrium isotherms and adsorption kinetics of lysozyme (LZ) on epichlorohydrin (Epi) cross-linked alginate-guar gum (Alg-GG) matrix were studied. Adsorption kinetics followed a pseudo-first-order model while the equilibrium isotherm could be represented by the Freundlich equation. The maximal amount of LZ adsorbed onto this matrix was around 2.4mg per g of hydrated matrix at pH 7.00. The adsorption mechanism was associated to a simple diffusion process with a weak columbic interaction between LZ and the matrix...
December 13, 2016: International Journal of Biological Macromolecules
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