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https://www.readbyqxmd.com/read/28438703/multilayered-membranes-with-tuned-well-arrays-to-be-used-as-regenerative-patches
#1
Nádia I Martins, Maria P Sousa, Catarina A Custódio, Vânia C Pinto, Paulo J Sousa, Graça Minas, Franck Cleymand, João F Mano
Membranes have been explored as patches in tissue repair and regeneration, most of them presenting a flat geometry or a patterned texture at the nano/micrometer scale. Herein, a new concept of a flexible membrane featuring well arrays forming pore-like environments to accommodate cell culture is proposed. The processing of such membranes using polysaccharides is based on the production of multilayers using the layer-by-layer methodology over a patterned PDMS substrate. The detached multilayered membrane exhibits a layer of open pores at one side and a total thickness of 38 ± 2...
April 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28438682/production-immobilization-and-thermodynamic-studies-of-free-and-immobilized-aspergillus-awamori-amylase
#2
Eman A Karam, Walaa A Abdel Wahab, Shireen A A Saleh, Mohamed E Hassan, Amany L Kansoh, Mona A Esawy
Enzyme cost, stability and its thermodynamic characteristics are the main criteria for industrial use. In this study, Aspergillus awamori amylase was constitutively produced using various agro-industrial wastes. Olive oil cake gave the highest activity (230 U/g). The amylase was partially purified to 2.81 fold purification. Immobilization was achieved using different carriers by covalent binding. The novel carrier Ca(+2) alginate (Alg) starch (St)/ polyethyleneimine (PEI)/glutaraldehyde (GA), showed the highest operational stability and was selected for further studies...
April 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28424550/doxorubicin-loaded-poly-lactic-co-glycolic-acid-nanoparticles-coated-with-chitosan-alginate-by-layer-by-layer-technology-for-antitumor-applications
#3
Fujuan Chai, Linlin Sun, Xinyi He, Jieli Li, Yuanfen Liu, Fei Xiong, Liang Ge, Thomas J Webster, Chunli Zheng
Natural polyelectrolyte multilayers of chitosan (CHI) and alginate (ALG) were alternately deposited on doxorubicin (DOX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) with layer by layer self-assembly to control drug release for antitumor activity. Numerous factors which influenced the multilayer growth on nano-colloidal particles were studied: polyelectrolyte concentration, NaCl concentration and temperature. Then the growth regime of the CHI/ALG multilayers was elucidated. The coated NPs were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction and a zeta potential analyzer...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28421389/preparation-and-biocompatibility-of-demineralized-bone-matrix-sodium-alginate-putty
#4
Yumin Zhang, Jianru Wang, Yanmiao Ma, Xiaojun Niu, Jianchun Liu, Lan Gao, Xiaoyan Zhai, Kaibo Chu, Bo Han, Liwang Yang, Jue Wang
Demineralized bone matrix (DBM) powder is widely used for bone regeneration due to its osteoinductivity and osteoconductivity. However, difficulties with handling, tendency to migrate from graft sites and lack of stability after surgery sometimes limit the clinical utility of this material. In this work, the possibility of using sodium alginate (ALG) carrier to deliver DBM powder was assessed. DBM-ALG putty with the DBM:ALG weight ratio of 5:5, 6:4, 7:3, 8:2 were prepared, respectively. The properties of the formed composite, including discrete degree, washout property, pH, equilibrium swelling as well as cytotoxicity in vivo, were adopted to ascertain the optimal ratio of DBM and ALG...
April 18, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28415545/engineering-bone-regeneration-with-novel-cell-laden-hydrogel-microfiber-injectable-calcium-phosphate-scaffold
#5
Yang Song, Chi Zhang, Ping Wang, Lin Wang, Chunyun Bao, Michael D Weir, Mark A Reynolds, Ke Ren, Liang Zhao, Hockin H K Xu
Cell-based tissue engineering is promising to create living functional tissues for bone regeneration. The implanted cells should be evenly distributed in the scaffold, be fast-released to the defect and maintain high viability in order to actively participate in the regenerative process. Herein, we report an injectable calcium phosphate cement (CPC) scaffold containing cell-encapsulating hydrogel microfibers with desirable degradability that could deliver cells in a timely manner and maintain cell viability...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415414/bionanocomposite-systems-based-on-montmorillonite-and-biopolymers-for-the-controlled-release-of-olanzapine
#6
Artur S Oliveira, Ana C S Alcântara, Sibele B C Pergher
Olanzapine (OLZ) is a drug that is used in the treatment of schizophrenia and other psychoses, and it belongs to the thienobenzodiazepine class. The OLZ molecule has low solubility decreasing bioavailability, but has high permeability in membrane biological being classified as a Class II drug substance according to the Biopharmaceutics Classification System. It was reported many side effects of administering OLZ orally. So, in order to increase the bioavailability of drug and possibly reducing some of side effects, this paper proposes a new material able to controllably release the drug in the body...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414151/targeted-antigen-delivery-to-dendritic-cell-via-functionalized-alginate-nanoparticles-for-cancer-immunotherapy
#7
Chuangnian Zhang, Gaona Shi, Ju Zhang, Huijun Song, Jinfeng Niu, Shengbin Shi, Pingsheng Huang, Yanming Wang, Weiwei Wang, Chen Li, Deling Kong
The purpose of the present study was to identify an "easy-to-adopt" strategy to enhance immune responses using functionalized alginate (ALG) nanoparticles (MAN-ALG/ALG=OVA NPs), which were prepared by CaCl2 cross-linking of two different types of ALG. The mannose (MAN) modified ALG (MAN-ALG) was used for dendritic cell targeting. The other component, composed of ovalbumin (OVA), a model antigen, is conjugated to ALG (ALG=OVA) via pH sensitive Schiff base bond. Grafting of alginate was demonstrated by FT-IR and (1)H NMR, while the morphological structure, particle size, Zeta potential of MAN-ALG/ALG=OVA NPs were measured using TEM and DLS...
April 13, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28398222/preparation-characterization-and-properties-of-alginate-poly-%C3%AE-glutamic-acid-composite-microparticles
#8
Zongrui Tong, Yu Chen, Yang Liu, Li Tong, Jiamian Chu, Kecen Xiao, Zhiyu Zhou, Wenbo Dong, Xingwu Chu
Alginate (Alg) is a renewable polymer with excellent hemostatic properties and biocapability and is widely used for hemostatic wound dressing. However, the swelling properties of alginate-based wound dressings need to be promoted to meet the requirements of wider application. Poly(γ-glutamic acid) (PGA) is a natural polymer with high hydrophility. In the current study, novel Alg/PGA composite microparticles with double network structure were prepared by the emulsification/internal gelation method. It was found from the structure characterization that a double network structure was formed in the composite microparticles due to the ion chelation interaction between Ca(2+) and the carboxylate groups of Alg and PGA and the electrostatic interaction between the secondary amine group of PGA and the carboxylate groups of Alg and PGA...
April 11, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28397862/a-robust-salt-tolerant-superoleophobic-alginate-graphene-oxide-aerogel-for-efficient-oil-water-separation-in-marine-environments
#9
Yuqi Li, Hui Zhang, Mizi Fan, Peitao Zheng, Jiandong Zhuang, Lihui Chen
Marine pollution caused by frequent oil spill accidents has brought about tremendous damages to marine ecological environment. Therefore, the facile large-scale preparation of three-dimensional (3D) porous functional materials with special wettability is in urgent demand. In this study, we report a low-cost and salt-tolerant superoleophobic aerogel for efficient oil/seawater separation. The aerogel is prepared through incorporating graphene oxide (GO) into alginate (ALG) matrix by using a facile combined freeze-drying and ionic cross-linking method...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396208/efficacy-of-a-high-potency-o1-manisa-foot-and-mouth-disease-vaccine-in-cattle-against-heterologous-challenge-with-a-field-virus-from-the-o-me-sa-ind-2001-lineage-collected-in-north-africa
#10
Emma Fishbourne, Anna B Ludi, Ginette Wilsden, Pip Hamblin, Bob Statham, Abdelghani Bin-Tarif, Emiliana Brocchi, Santina Grazioli, Aldo Dekker, Phaedra Eblé, Donald P King
Outbreaks of foot-and-mouth disease (FMD) in North Africa (2013) and the Gulf States (2013) of the Middle East have been caused by a FMD viral lineage (O/ME-SA/Ind-2001) that was before 2013 restricted to the Indian Sub-continent. This study was undertaken to assess the in vivo efficacy of a FMD virus emergency vaccine type O1 Manisa against heterologous challenge with a representative field virus (O/ALG/3/2014) from this emerging lineage. This widely available vaccine was selected since in vitro vaccine-matching results gave inconclusive results as to whether or not it would be protective...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28384617/the-study-of-angiogenesis-stimulated-by-multivalent-peptide-ligand-modified-alginate
#11
Bicui Wang, Wei Wang, Yanyan Yu, Yingxue Zhang, Jingwei Zhang, Zhi Yuan
Enhancing the affinity of scaffolds for endothelial cell (EC) is a crucial procedure for promoting angiogenesis in tissue engineering. In this work, to achieve stronger binding affinity with ECs, the peptide sequence REDV was conjugated onto gold nanoparticles (AuNPs) to construct a multivalent ligand (cREDV). Then, the EC adhesion and proliferation were studied to determine ligand affinity for ECs in vitro. The results show that the cREDV-modified alginate (cREDV-ALG) surface exhibited a selective adhesion to human umbilical vein endothelial cells (HUVECs) compared with NIH 3T3 cells...
March 25, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28372295/comparison-of-msc-properties-in-two-different-hydrogels-impact-of-mechanical-properties
#12
Hao Yu, Ghislaine Cauchois, Nicolas Louvet, Yun Chen, Rachid Rahouadj, Céline Huselstein
Once articular cartilage is damaged, it has poor ability to heal. At present, alginate-based hydrogels have 3D-dimensional physical structures with great potential for applications in carilage tissue engineering. For osteochondral defect, it will be necessary to use stratified scaffold to mimic zonal organization of cartilage. This study aims to compare the characteristics of alginate (Alg)/hyaluronic acid (HA) hydrogels which will mimic cartilage with alginate (Alg)/hydroxyapatite (Hap) hydrogels which will mimic subchondral bone...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28356731/co-delivery-nanoparticles-with-characteristics-of-intracellular-precision-release-drugs-for-overcoming-multidrug-resistance
#13
DanDan Zhang, Yan Yan Kong, Jia Hui Sun, Shao Jie Huo, Min Zhou, Yi Ling Gui, Xu Mu, Huan Chen, Shu Qin Yu, Qian Xu
Combination chemotherapy in clinical practice has been generally accepted as a feasible strategy for overcoming multidrug resistance (MDR). Here, we designed and successfully prepared a co-delivery system named S-D1@L-D2 NPs, where denoted some smaller nanoparticles (NPs) carrying a drug doxorubicin (DOX) were loaded into a larger NP containing another drug (vincristine [VCR]) via water-in-oil-in-water double-emulsion solvent diffusion-evaporation method. Chitosan-alginate nanoparticles carrying DOX (CS-ALG-DOX NPs) with a smaller diameter of about 20 nm formed S-D1 NPs; vitamin E D-α-tocopheryl polyethylene glycol 1000 succinate-modified poly(lactic-co-glycolic acid) nanoparticles carrying VCR (TPGS-PLGA-VCR NPs) with a larger diameter of about 200 nm constituted L-D2 NPs...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28322231/alix-is-required-during-development-for-normal-growth-of-the-mouse-brain
#14
Marine H Laporte, Christine Chatellard, Victoria Vauchez, Fiona J Hemming, Jean-Christophe Deloulme, Frédérique Vossier, Béatrice Blot, Sandrine Fraboulet, Rémy Sadoul
Alix (ALG-2 interacting protein X) drives deformation and fission of endosomal and cell surface membranes and thereby intervenes in diverse biological processes including cell proliferation and apoptosis. Using embryonic fibroblasts of Alix knock-out mice, we recently demonstrated that Alix is required for clathrin-independent endocytosis. Here we show that mice lacking Alix suffer from severe reduction in the volume of the brain which affects equally all regions examined. The cerebral cortex of adult animals shows normal layering but is reduced in both medio-lateral length and thickness...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303080/random-walks-on-simplicial-complexes-and-harmonics
#15
Sayan Mukherjee, John Steenbergen
In this paper, we introduce a class of random walks with absorbing states on simplicial complexes. Given a simplicial complex of dimension d, a random walk with an absorbing state is defined which relates to the spectrum of the k-dimensional Laplacian for 1 ≤ k ≤ d. We study an example of random walks on simplicial complexes in the context of a semi-supervised learning problem. Specifically, we consider a label propagation algorithm on oriented edges, which applies to a generalization of the partially labelled classification problem on graphs...
September 2016: Random Structures & Algorithms
https://www.readbyqxmd.com/read/28300556/alg2-regulates-glioblastoma-cell-proliferation-migration-and-tumorigenicity
#16
Dunke Zhang, Feng Wang, Yi Pang, Erhu Zhao, Sunqin Zhu, Fei Chen, Hongjuan Cui
Apoptosis-linked gene-2 (ALG-2), also known as programmed cell death 6 (PDCD6), has recently been reported to be aberrantly expressed in various tumors and required for tumor cell viability. The aim of the present study was to investigate whether ALG-2 plays a crucial role in tumor cell proliferation, migration and tumorigenicity. In this study, we examined the expression of PDCD6 in glioblastoma cell lines and found that ALG-2 was generally expressed in glioblastoma cell lines. We also performed an analysis of an online database and found that high expression of ALG-2 was associated with poor prognosis (p = 0...
April 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28278043/injectable-conductive-collagen-alginate-polypyrrole-hydrogels-as-a-biocompatible-system-for-biomedical-applications
#17
Farinaz Ketabat, Akbar Karkhaneh, Rouhollah Mehdinavaz Aghdam, Seyed Hossein Ahmadi Tafti
Recently, Injectable Conducting Hydrogel (ICH) systems have gained much attention for tissue engineering and regenerative medicine. These systems can promote the regeneration of tissues responding to electrical responses. In this study, a novel ICH system was introduced. To achieve this system, firstly, a soluble non-toxic polypyrrole (PPy) synthesized by grafting pyrrole on alginate (Alg) backbone (Alg-graft-PPy), and then, ICH systems were prepared by the given ratios of Alg-graft-PPy, Alg, and collagen (Col)...
March 28, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28225139/redox-and-light-responsive-alginate-nanoparticles-as-effective-drug-carriers-for-combinational-anticancer-therapy
#18
Chuangnian Zhang, Gaona Shi, Ju Zhang, Jinfeng Niu, Pingsheng Huang, Zhihong Wang, Yanming Wang, Weiwei Wang, Chen Li, Deling Kong
Nanoparticles have been extensively explored as effective means to deliver chemotherapeutic agents or photosensitizers for chemotherapy or photodynamic therapy (PDT) against cancer. In the present work, pheophorbide A (PheoA), a hydrophobic photosensitizer, was conjugated via a redox-sensitive disulfide linkage to alginate (PheoA-ALG). Anticancer agent, doxorubicin (DOX), was also loaded within the PheoA-ALG nanoparticles (DOX/PheoA-ALG NPs) and used as drug carriers for combinational antitumor treatment. The DOX/PheoA-ALG NPs were spherical in shape with a uniform diameter of approximately 210 nm...
February 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28181101/preparation-of-hydroxyapatite-collagen-injectable-bone-paste-with-an-anti-washout-property-utilizing-sodium-alginate-part-1-influences-of-excess-supplementation-of-calcium-compounds
#19
Taira Sato, Masanori Kikuchi, Mamoru Aizawa
The anti-washout property, viscosity, and cytocompatibility to an osteoblastic cell line, MG-63, of anti-washout pastes were investigated. Mixing a hydroxyapatite/collagen bone-like nanocomposite (HAp/Col), an aqueous solution of sodium alginate (Na-Alg), which is a paste hardening and lubricant agent, and supplementation of calcium carbonate or calcium citrate (Ca-Cit) as a calcium resource for the hardening reaction realized an injectable bone paste. Adding Ca-Cit at a concentration greater than eight times the Ca(2+) ion concentration to Na-Alg improved the anti-washout property...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28170076/biomimetic-sulphated-alginate-hydrogels-suppress-il-1%C3%AE-induced-inflammatory-responses-in-human-chondrocytes
#20
Ø Arlov, M Steinwachs, G Skjåk-Bræk, M Zenobi-Wong
Loss of articular cartilage from ageing, injury or degenerative disease is commonly associated with inflammation, causing pain and accelerating degradation of the cartilage matrix. Sulphated glycosaminoglycans (GAGs) are involved in the regulation of immune responses in vivo, and analogous polysaccharides are currently being evaluated for tissue engineering matrices to form a biomimetic environment promoting tissue growth while suppressing inflammatory and catabolic activities. Here, we characterise physical properties of sulphated alginate (S-Alg) gels for use in cartilage engineering scaffolds, and study their anti-inflammatory effects on encapsulated chondrocytes stimulated with IL-1β...
February 7, 2017: European Cells & Materials
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