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https://www.readbyqxmd.com/read/29333652/establishment-and-characterization-of-a-brain-cell-line-from-kelp-grouper-epinephelus-moara
#1
X F Liu, Y H Wu, S N Wei, N Wang, Y Z Li, N W Zhang, P F Li, Q W Qin, S L Chen
A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30° C, with the maximum growth between 24 and 30° C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45...
January 14, 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29329336/effective-dispersal-and-density-dependence-in-mesophotic-macroalgal-forests-insights-from-the-mediterranean-species-cystoseira-zosteroides
#2
Pol Capdevila, Cristina Linares, Eneko Aspillaga, Joan Lluís Riera, Bernat Hereu
Dispersal and recruitment are fundamental processes for population recovery following disturbances in sessile species. While both processes are well understood for many terrestrial species, they still remain poorly resolved for some macroalgal species. Here we experimentally investigated the effective dispersal and recruit survival of a mesophotic Mediterranean fucoid, Cystoseira zosteroides. In three isolated populations, four sets of settlement collectors were placed at increasing distances (from 0 to 10 m) and different orientations (North, South, East and West)...
2018: PloS One
https://www.readbyqxmd.com/read/29317025/electrochemical-sensing-platform-based-on-kelp-derived-hierarchical-meso-macroporous-carbons
#3
Yashuang Hei, Xiqian Li, Xiao Zhou, Jingju Liu, Mimi Sun, Tianze Sha, Cuixing Xu, Wanyi Xue, Xiangjie Bo, Ming Zhou
In this paper, kelp (Laminaria japonica), as a kind of abundant biomass, is used as the precursor for the preparation of kelp-derived hierarchical meso-macroporous carbons (K-dHMMCs) through the carbonization under nitrogen (N2) atmosphere at high temperature. The K-dHMMCs exhibits the unique structure with high specific surface area of 416.02 m2 g-1, large pore volume of 0.24 cm3 g-1, the hierarchical meso-macroporous size distribution centered at 2, 12 and 82 nm and high density of defective sites, enabling K-dHMMCs attractive for the electrocatalysis...
March 20, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29299287/diet-reveals-links-between-morphology-and-foraging-in-a-cryptic-temperate-reef-fish
#4
Natalia S Winkler, Maite Paz-Goicoechea, Robert W Lamb, Alejandro Pérez-Matus
Predators select prey so as to maximize energy and minimize manipulation time. In order to reduce prey detection and handling time, individuals must actively select their foraging space (microhabitat) and populations exhibit morphologies that are best suited for capturing locally available prey. We explored how variation in diet correlates with habitat type, and how these factors influence key morphological structures (mouth gape, eye diameter, fin length, fin area, and pectoral fin ratio) in a common microcarnivorous cryptic reef fish species, the triplefin Helcogrammoides cunninghami...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29298307/persistent-differences-between-coastal-and-offshore-kelp-forest-communities-in-a-warming-gulf-of-maine
#5
Jon D Witman, Robert W Lamb
Kelp forests provide important ecosystem services, yet coastal kelp communities are increasingly altered by anthropogenic impacts. Kelp forests in remote, offshore locations may provide an informative contrast due to reduced impacts from local stressors. We tested the hypothesis that shallow kelp assemblages (12-15 m depth) and associated fish and benthic communities in the coastal southwest Gulf of Maine (GOM) differed significantly from sites on Cashes Ledge, 145 km offshore by sampling five coastal and three offshore sites at 43...
2018: PloS One
https://www.readbyqxmd.com/read/29281144/size-growth-and-density-data-for-shallow-water-sea-urchins-from-mexico-to-the-aleutian-islands-alaska-1956-2016
#6
Thomas A Ebert, Louis M Barr, James L Bodkin, Dirk Burcham, Dominique Bureau, Henry S Carson, Nancy L Caruso, Jennifer E Caselle, Jeremy T Claisse, Sabrina Clemente, Kathryn Davis, Paul M Detwiler, John D Dixon, David O Duggins, John M Engle, James A Estes, Scott D Groth, Benjamin M Grupe, Peter Halmay, Kyle P Hebert, José C Hernández, Laura J Jurgens, Peter E Kalvass, Michael C Kenner, Brenda Kenner, David J Kushner, Lynn C Lee, David L Leighton, Gabriela Montaño-Moctezuma, J Eric Munk, Irma Olguin, Julio S Palleiro, David O Parker, John S Pearse, Daniel J Pondella, Laura Rogers-Bennett, Stephen C Schroeter, A Ole Shelton, Jorge Sonnenholzner, Ian K Taniguchi, Glenn R VanBlaricom, Jane C Watson, Benjamin P Weitzman, Jonathan P Williams, Jennifer Yakimishyn, Zane Zhang
Size, growth, and density have been studied for North American Pacific coast sea urchins Strongylocentrotus purpuratus, S. droebachiensis, S. polyacanthus, Mesocentrotus (Strongylocentrotus) franciscanus, Lytechinus pictus, Centrostephanus coronatus, and Arbacia stellata by various workers at diverse sites and for varying lengths of time from 1956 to present. Numerous peer-reviewed publications have used some of these data but some data have appeared only in graduate theses or the gray literature. There also are data that have never appeared outside original data sheets...
December 27, 2017: Ecology
https://www.readbyqxmd.com/read/29234336/evolution-and-expansion-of-the-prokaryote-like-lipoxygenase-family-in-the-brown-alga-saccharina-japonica
#7
Linhong Teng, Wentao Han, Xiao Fan, Dong Xu, Xiaowen Zhang, Simon M Dittami, Naihao Ye
Lipoxygenase (LOX) plays important roles in fatty acid oxidation and lipid mediator biosynthesis. In this study, we give first insights into brown algal LOX evolution. Whole genome searches revealed four, three, and eleven LOXs in Ectocarpus siliculosus, Cladosiphon okamuranus, and Saccharina japonica, respectively. In phylogenetic analyses, LOXs from brown algae form a robust clade with those from prokaryotes, suggesting an ancestral origin and slow evolution. Brown algal LOXs were divided into two clades, C1 and C2 in a phylogenetic tree...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29222652/development-of-a-kelp-powder-thallus-laminariae-standard-reference-material
#8
Lee L Yu, Joseph F Browning, Carolyn Q Burdette, George C Caceres, Kaitlyn D Chieh, W Clay Davis, Brittany L Kassim, Stephen E Long, Karen E Murphy, Rabia Oflaz, Rick L Paul, Katherine E Sharpless, Laura J Wood, James H Yen, Rolf Zeisler
A Standard Reference Material (SRM) of seaweed, SRM 3232 Kelp Powder (Thallus laminariae) has been developed to support food and dietary supplement measurements in compliance with the Food Safety Modernization Act (FSMA) and the Dietary Supplement Health and Education Act of 1994 (DSHEA). The material was characterized for nutritional minerals, arsenic species, isomers of vitamin K1, proximates, and toxic elements. Kelp is a rich source of vitamins and minerals, and it is an excellent source of dietary iodine...
December 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29220368/increased-sediment-load-during-a-large-scale-dam-removal-changes-nearshore-subtidal-communities
#9
Stephen P Rubin, Ian M Miller, Melissa M Foley, Helen D Berry, Jeffrey J Duda, Benjamin Hudson, Nancy E Elder, Matthew M Beirne, Jonathan A Warrick, Michael L McHenry, Andrew W Stevens, Emily F Eidam, Andrea S Ogston, Guy Gelfenbaum, Rob Pedersen
The coastal marine ecosystem near the Elwha River was altered by a massive sediment influx-over 10 million tonnes-during the staged three-year removal of two hydropower dams. We used time series of bathymetry, substrate grain size, remotely sensed turbidity, scuba dive surveys, and towed video observations collected before and during dam removal to assess responses of the nearshore subtidal community (3 m to 17 m depth). Biological changes were primarily driven by sediment deposition and elevated suspended sediment concentrations...
2017: PloS One
https://www.readbyqxmd.com/read/29216296/correction-the-potential-role-of-kelp-forests-on-iodine-speciation-in-coastal-seawater
#10
Jennifer Gonzales, Teresa Tymon, Frithjof C Küpper, Matthew S Edwards, Carl J Carrano
[This corrects the article DOI: 10.1371/journal.pone.0180755.].
2017: PloS One
https://www.readbyqxmd.com/read/29214431/establishment-and-characterization-of-a-kidney-cell-line-from-kelp-grouper-epinephelus-moara
#11
Xiao-Feng Liu, Ya-Hong Wu, Shi-Na Wei, Na Wang, Peng-Fei Li, Yang-Zhen Li, Nian-Wei Zhang, Qi-Wei Qin, Song-Lin Chen
A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 °C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 °C, with optimal growth at 24 °C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42...
December 6, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29213185/crude-fucoidan-content-in-two-north-atlantic-kelp-species-saccharina-latissima-and-laminaria-digitata-seasonal-variation-and-impact-of-environmental-factors
#12
Annette Bruhn, Tina Janicek, Dirk Manns, Mette Møller Nielsen, Thorsten Johannes Skovbjerg Balsby, Anne S Meyer, Michael Bo Rasmussen, Xiaoru Hou, Bodo Saake, Cordula Göke, Anne Belinda Bjerre
Fucoidans are sulphated fucose-rich polysaccharides predominantly found in the cell walls of brown algae. The bioactive properties of fucoidans attract increasing interest from the medico-pharmaceutical industries and may drive an increase in demand of brown algae biomass. In nature, the biochemical composition of brown algae displays a seasonal fluctuation driven by environmental factors and endogenous rhythms. To cultivate and harvest kelps with high yields of fucoidans, knowledge is needed on seasonal variation and impact of environmental conditions on the fucoidan content of brown algae...
2017: Journal of Applied Phycology
https://www.readbyqxmd.com/read/29183281/optimizing-and-evaluating-the-reconstruction-of-metagenome-assembled-microbial-genomes
#13
Bhavya Papudeshi, J Matthew Haggerty, Michael Doane, Megan M Morris, Kevin Walsh, Douglas T Beattie, Dnyanada Pande, Parisa Zaeri, Genivaldo G Z Silva, Fabiano Thompson, Robert A Edwards, Elizabeth A Dinsdale
BACKGROUND: Microbiome/host interactions describe characteristics that affect the host's health. Shotgun metagenomics includes sequencing a random subset of the microbiome to analyze its taxonomic and metabolic potential. Reconstruction of DNA fragments into genomes from metagenomes (called metagenome-assembled genomes) assigns unknown fragments to taxa/function and facilitates discovery of novel organisms. Genome reconstruction incorporates sequence assembly and sorting of assembled sequences into bins, characteristic of a genome...
November 28, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29170569/fine-scale-population-genetic-structure-of-sugar-kelp-saccharina-latissima-laminariales-phaeophyceae-in-eastern-maine-usa
#14
Timothy S Breton, Jeremy C Nettleton, Brennah O'Connell, Margaret Bertocci
There is an interest to develop sugar kelp (Saccharina latissima) cultivation in the rural, eastern Maine region of the USA. Future farming efforts would benefit from an understanding of the genetic diversity and population structure of kelp, to inform management and conservation, and to identify genetic resources. The purpose of the present study was to characterize the fine-scale population genetic structure of kelp in eastern Maine, using twelve microsatellite loci. A total of 188 samples were genotyped from five sampling locations...
2018: Phycologia
https://www.readbyqxmd.com/read/29158994/detection-of-bacterial-contaminants-and-hybrid-sequences-in-the-genome-of-the-kelp-saccharina-japonica-using-taxoblast
#15
Simon M Dittami, Erwan Corre
Modern genome sequencing strategies are highly sensitive to contamination making the detection of foreign DNA sequences an important part of analysis pipelines. Here we use Taxoblast, a simple pipeline with a graphical user interface, for the post-assembly detection of contaminating sequences in the published genome of the kelp Saccharina japonica. Analyses were based on multiple blastn searches with short sequence fragments. They revealed a number of probable bacterial contaminations as well as hybrid scaffolds that contain both bacterial and algal sequences...
2017: PeerJ
https://www.readbyqxmd.com/read/29143457/microhaplotypes-provide-increased-power-from-short-read-dna-sequences-for-relationship-inference
#16
Diana S Baetscher, Anthony J Clemento, Thomas C Ng, Eric C Anderson, John Carlos Garza
The accelerating rate at which DNA sequence data is now generated by high-throughput sequencing instruments provides both opportunities and challenges for population genetic and ecological investigations of animals and plants. We show here how the common practice of calling genotypes from a single SNP per sequenced region ignores substantial additional information in the phased short-read sequences that are provided by these sequencing instruments. We target sequenced regions with multiple SNPs in kelp rockfish (Sebastes atrovirens) to determine "microhaplotypes" and then call these microhaplotypes as alleles at each locus...
November 16, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29124859/sympatric-kelp-species-share-a-large-portion-of-their-surface-bacterial-communities
#17
Matthew A Lemay, Patrick T Martone, Patrick Keeling, Jenn M Burt, Kira A Krumhansl, Rhea D Sanders, Laura Wegener Parfrey
Kelp forest ecosystems are biodiversity hotspots, providing habitat for dense assemblages of marine organisms and nutrients for marine and terrestrial food webs. The surfaces of kelps support diverse microbial communities that facilitate the transfer of carbon from algal primary production to higher trophic levels. We quantified the diversity of bacteria on the surfaces of eight sympatric kelp species from four sites in British Columbia. Kelp-associated bacterial communities are significantly different from their environment, even though 86% of their bacterial taxa are shared with seawater and 97% are shared with rocky substrate...
November 10, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29101878/biochars-derived-from-wasted-marine-macro-algae-saccharina-japonica-and-sargassum-fusiforme-and-their-potential-for-heavy-metal%C3%A2-removal-in-aqueous-solution
#18
Kyung-Min Poo, Eun-Bi Son, Jae-Soo Chang, Xianghao Ren, Yun-Jung Choi, Kyu-Jung Chae
For the purpose of reusing wasted marine macro-algae generated during cultivation, harvesting, processing and selling processes, biochars derived from Saccharina japonica (known as kelp) and Sargassum fusiforme (known as hijikia) were characterized and their removal capacities for Cu, Cd, and Zn in aqueous solution were assessed. Feedstocks, S. japonica, S. fusiforme, and also pinewood sawdust as a control, were pyrolyzed at 250, 400, 500, 600 and 700 °C. In evaluating heavy metal removal capacities, SJB (S...
November 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29101467/scale-specific-drivers-of-kelp-forest-communities
#19
Thomas Lamy, Daniel C Reed, Andrew Rassweiler, David A Siegel, Li Kui, Tom W Bell, Rachel D Simons, Robert J Miller
Identifying spatial scales of variation in natural communities and the processes driving them is critical for obtaining a predictive understanding of biodiversity. In this study, we focused on diverse communities inhabiting productive kelp forests on shallow subtidal rocky reefs in southern California, USA. We combined long-term community surveys from 86 sites with detailed environmental data to determine what structures assemblages of fishes, invertebrates and algae at multiple spatial scales. We identified the spatial scales of variation in species composition using a hierarchical analysis based on eigenfunctions, and assessed how sea surface temperature (SST), water column chlorophyll, giant kelp biomass, wave exposure and potential propagule delivery strength contributed to community variation at each scale...
November 3, 2017: Oecologia
https://www.readbyqxmd.com/read/29093219/predators-indirectly-induce-stronger-prey-through-a-trophic-cascade
#20
Arie J P Spyksma, Nick T Shears, Richard B Taylor
Many prey species induce defences in direct response to predation cues. However, prey defences could also be enhanced by predators indirectly via mechanisms that increase resource availability to prey, e.g. trophic cascades. We evaluated the relative impacts of these direct and indirect effects on the mechanical strength of the New Zealand sea urchin Evechinus chloroticus We measured crush-resistance of sea urchin tests (skeletons) in (i) two marine reserves, where predators of sea urchins are relatively common and have initiated a trophic cascade resulting in abundant food for surviving urchins in the form of kelp, and (ii) two adjacent fished areas where predators and kelps are rare...
November 15, 2017: Proceedings. Biological Sciences
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