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AMPK AND Sirt1

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https://www.readbyqxmd.com/read/29771428/ampk-regulates-energy-metabolism-through-the-sirt1-signaling-pathway-to-improve-myocardial-hypertrophy
#1
H-W Dong, L-F Zhang, S-L Bao
OBJECTIVE: We investigated the correlations of adenosine monophosphate-activated protein kinase (AMPK), Silence information regulator 1 (SIRT1) and energy metabolism with myocardial hypertrophy. MATERIALS AND METHODS: Myocardial hypertrophy experimental model was established via transverse aortic constriction (TAC)-induced myocardial hypertrophy and phenylephrine (PE)-induced hypertrophic myocardial cell culture. After activation of AMPK, the messenger ribonucleic acid (mRNA) expressions in myocardial tissue- and myocardial cell hypertrophy-related genes, atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC), were detected...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29752949/the-change-of-nuclear-lc3-distribution-in-acute-myeloid-leukemia-cells
#2
Guo Wenjian, Jin Jingrui, Pan Jiajia, Yao Rongxing, Li Xia, Huang Xin, Ma Zhixing, Huang Sujuan, Yan Xiao, Jin Jie, Dong Aishu
Making sure the change of nuclear LC3 distribution in the autophagy of acute myeloid leukemia (AML) cell and finding out the regulation mechanism may lead to a breakthrough for killing AML cells. Western blots were performed to assess the expression of autophagy proteins. Changes in the LC3 distribution were monitored by immunofluorescence assays together with western blots, and the expression levels of Sirt1, DOR, Beclin1, HMGB1, and AMPK mRNA were detected via fluorescent quantitative PCR. The effects of Sirt1 and DOR on cell proliferation and survival were analyzed by MTT, flow cytometry, and western blotting assays...
May 9, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29723566/triptolide-induced-mitochondrial-damage-dysregulates-fatty-acid-metabolism-in-mouse-sertoli-cells
#3
Yisen Cheng, Gaojian Chen, Li Wang, Jiamin Kong, Ji Pan, Yue Xi, Feihai Shen, Zhiying Huang
Triptolide is a major active ingredient of tripterygium glycosides, used for the therapy of immune and inflammatory diseases. However, its clinical applications are limited by severe male fertility toxicity associated with decreased sperm count, mobility and testicular injures. In this study, we determined that triptoide-induced mitochondrial dysfunction triggered reduction of lactate and dysregulation of fatty acid metabolism in mouse Sertoli cells. First, triptolide induced mitochondrial damage through the suppressing of proliferator-activated receptor coactivator-1 alpha (PGC-1α) activity and protein...
April 30, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29706079/modulation-of-ampk-dependent-lipogenesis-mediated-by-p2x7r-nlrp3-inflammasome-activation-contributes-to-the-amelioration-of-alcoholic-liver-steatosis-by-dihydroquercetin
#4
Yu Zhang, Quan Jin, Xia Li, Jiang Min, Ben-Wen Cui, Kai-Li Xia, Yan-Ling Wu, Li-Hua Lian, Ji-Xing Nan
Dihydroquercetin (TAX) is the most abundant dihydroflavone found in onions, milk thistle and Douglas fir bark. We investigated whether TAX could inhibit the lipid accumulation in alcoholic liver steatosis in vivo and in vitro. An in vivo model was established by intragastrically treating mice with ethanol, and an in vitro model was created by treating HepG2 cells with ethanol. TAX regulated SREBP1 and ACC expression via elevating LKB1/AMPK phosphorylation. Also, TAX upregulated SIRT1 expression, which suppressed by ethanal intake...
April 28, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29705966/molecular-cloning-of-adipose-triglyceride-lipase-atgl-gene-from-blunt-snout-bream-and-its-expression-after-lps-induced-tnf-%C3%AE-factor
#5
Yong-Jun Dai, Wen-Bin Liu, Xiang-Fei Li, Man Zhou, Chao Xu, Yu Qian, Guang-Zhen Jiang
The aims of the present study were to clone the full-length cDNA of adipose triglyceridelipase (ATGL) and to analyze its expression after lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α). The cDNA obtained covered 1801 bp with an open reading frame of 1500 bp encoding 499 amino acids. Sequence alignment and phylogenetic analysis show the best identity with Cyprinus carpio (86%). The ATGL protein shared a highly conserved 169-amino acid patatin domain, containing a glycine-rich motif, an active serine hydrolase motif, and an aspartic active site...
April 29, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29696666/randomised-clinical-trial-a-leucine-metformin-sildenafil-combination-ns-0200-vs-placebo-in-patients-with-non-alcoholic-fatty-liver-disease
#6
N Chalasani, R Vuppalanchi, M Rinella, M S Middleton, M S Siddiqui, A S Barritt, O Kolterman, O Flores, C Alonso, M Iruarrizaga-Lejarreta, R Gil-Redondo, C B Sirlin, M B Zemel
BACKGROUND: Sirtuin 1 (Sirt1) is suppressed in non-alcoholic fatty liver disease (NAFLD), while its' stimulation or overexpression results in reduced disease severity in pre-clinical NAFLD models. Leucine allosterically activates Sirt1 and synergise with other Sirt/AMPK/NO pathway activators. We developed a triple combination of leucine, metformin and sildenafil (NS-0200), which was effective in a mouse model of non-alcoholic steatohepatitis (NASH). AIM: To report the results from a Phase 2, randomised clinical trial of of NS-0200 in 91 subjects with NAFLD (liver fat ≥15% by magnetic resonance imaging-proton-density fat fraction (MRI-PDFF))...
April 25, 2018: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29693104/c-phycocyanin-inhibits-hepatic-gluconeogenesis-and-increases-glycogen-synthesis-via-activating-akt-and-ampk-in-insulin-resistance-hepatocytes
#7
Zhiheng Ren, Zhifei Xie, Danni Cao, Mufeng Gong, Lei Yang, Zhu Zhou, Yu Ou
C-Phycocyanin (C-PC), a kind of blue protein isolated from Spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. In the present study, we investigated the effects and underlying mechanisms of C-PC on gluconeogenesis and glycogenesis in insulin resistant hepatocytes. Insulin resistance was induced by high glucose (HG) in human hepatocellular carcinoma (HepG2) cells. C-PC ameliorated glucose production and phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) expression in HG-induced insulin resistant HepG2 cells...
April 25, 2018: Food & Function
https://www.readbyqxmd.com/read/29681936/metformin-regulating-mir-34a-pathway-to-inhibit-egr1-in-rat-mesangial-cells-cultured-with-high-glucose
#8
Can Wu, Ningning Qin, Huiwen Ren, Min Yang, Shuang Liu, Qiuyue Wang
Background: Activating AMPK α negatively regulates Egr1 to inhibit inflammatory cytokines in high glucose. miR-34a inhibition increases phosphorylated AMPK α through mediating SIRT1 to suppress the development of fatty liver. Aim of the Study: To clarify the function of Egr1 on the inflammation and fibrosis in high glucose-cultured MCs, as well as to explore the effects of metformin on miR-34a pathway and Egr1 expression. Methods: We transfected MCs with miR-34a inhibitor...
2018: International Journal of Endocrinology
https://www.readbyqxmd.com/read/29679925/tocotrienol-rich-fraction-supplementation-reduces-hyperglycemia-induced-skeletal-muscle-damage-through-regulation-of-insulin-signaling-and-oxidative-stress-in-type-2-diabetic-mice
#9
Heaji Lee, Yunsook Lim
Chronic hyperglycemia induces impairment of muscle growth and development of diabetes mellitus (DM). Since skeletal muscle is the major site for disposal of ingested glucose, impaired glucose metabolism causes imbalance between protein synthesis and degradation which adversely affects physical mobility. In this study, we investigated the effect of tocotrienol-rich fraction (TRF) supplementation on skeletal muscle damage in diabetic mice. Diabetes was induced by a high-fat diet with streptozotocin (STZ) injection (100 mg/kg) in male C57BL/6J mice...
March 21, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29674003/tanshinone-iia-suppresses-fc%C3%AE%C2%B5ri-mediated-mast-cell-signaling-and-anaphylaxis-by-activation-of-the-sirt1-lkb1-ampk-pathway
#10
Xian Li, Soon Jin Park, Fansi Jin, Yifeng Deng, Ju Hye Yang, Jae-Hoon Chang, Dong-Young Kim, Jung-Ae Kim, Youn Ju Lee, Makoto Murakami, Kun Ho Son, Hyeun Wook Chang
AMP-activated protein kinase (AMPK) and its upstream mediators liver kinase B1 (LKB1) and sirtuin 1 (Sirt1) are generally known as key regulators of metabolism. We have recently reported that the AMPK pathway negatively regulates mast cell activation and anaphylaxis. Tanshinone IIA (Tan IIA), an active component of Salvia miltiorrhiza extract that is currently used for the treatment of cardiovascular and cerebrovascular diseases, shows anti-diabetic activity and improves insulin resistance in db/db mice through activation of AMPK...
June 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29659128/activation-of-dna-demethylases-attenuates-aging-associated-arterial-stiffening-and-hypertension
#11
Kai Chen, Zhongjie Sun
DNA methylation increases with age. The objective of this study was to investigate whether compound H, a potential activator of DNA demethylases, attenuates aging-related arterial stiffness and hypertension. Aged mice (24-27 months) and adult mice (12 months) were used. Pulse wave velocity (PWV), a direct measure of arterial stiffness, and blood pressure (BP) were increased significantly in aged mice. Notably, daily treatments with compound H (15 mg/kg, IP) for 2 weeks significantly attenuated the aging-related increases in PWV and BP...
April 16, 2018: Aging Cell
https://www.readbyqxmd.com/read/29656453/chlorogenic-acid-protects-against-oxldl-induced-oxidative-damage-and-mitochondrial-dysfunction-by-modulating-sirt1-in-endothelial-cells
#12
Kun-Ling Tsai, Ching-Hsia Hung, Shih-Hung Chan, Pei-Ling Hsieh, Hsiu-Chung Ou, Yung-Hsin Cheng, Pei-Ming Chu
SCOPE: Endothelial dysfunction is an important mechanism in the development of atherosclerosis and is thought to be critical for predicting cardiovascular diseases. Previous reports suggested that Chlorogenic acid (CGA) is a potent antioxidant and anti-inflammatory compound. The molecular mechanisms underlying the inhibitory effects of CGA on oxLDL-induced oxidative injuries in human endothelial cells are still largely unknown. This study aimed to test the hypothesis that CGA protects against oxLDL-facilitated oxidative stress by upregulating SIRT1 and to explore the role of AMPK/PGC-1 pathway and mitochondrial biogenesis...
April 15, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29644357/the-inhibitory-effect-of-manuka-honey-on-human-colon-cancer-hct-116-and-lovo-cell-growth-part-2-induction-of-oxidative-stress-alteration-of-mitochondrial-respiration-and-glycolysis-and-suppression-of-metastatic-ability
#13
Sadia Afrin, Francesca Giampieri, Massimiliano Gasparrini, Tamara Y Forbes-Hernández, Danila Cianciosi, Patricia Reboredo-Rodriguez, Piera Pia Manna, Jiaojiao Zhang, Josè L Quiles, Maurizio Battino
Despite its high content of phenolic compounds, the chemopreventive activity of Manuka honey (MH) is still elusive. The aim of the present work was to evaluate the effects of MH on oxidative stress, antioxidant enzymes, cellular metabolism and the metastatic ability in HCT-116 and LoVo cells, paying particular attention to the molecular mechanisms involved. We observed a strong induction of oxidative stress after MH treatment since it augmented the accumulation of reactive oxygen species and increased the damage to proteins, lipids and DNA...
April 12, 2018: Food & Function
https://www.readbyqxmd.com/read/29609691/chrysophanol-ameliorates-high-fat-diet-induced-obesity-and-inflammation-in-neonatal-rats
#14
Jie Zhang, Hua Kang, Lifang Wang, Xiaoyan Zhao
Chrysophanol is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Chinese Medicine. Though several studies have indicated numerous features of chrysophanol, no study has yet reported the effect of chrysophanol on juvenile obesity. In this study, we tried to identify the anti-obesity effects of chrysophanol by using high-fat diet (HFD)-induced rats as in vivo models. In HFD rats, chrysophanol treatment decreased body weight, blood glucose and the blood level of triglyceride (TG), and enhanced the level of high-density lipoprotein-cholesterol (HDL-C)...
April 2, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29603067/secretome-of-differentiated-pc12-cells-restores-the-monocrotophos-induced-damages-in-human-mesenchymal-stem-cells-and-shsy-5y-cells-role-of-autophagy-and-mitochondrial-dynamics
#15
A Srivastava, S Singh, C S Rajpurohit, P Srivastava, A Pandey, D Kumar, V K Khanna, A B Pant
A perturbed cellular homeostasis is a key factor associated with xenobiotic exposure resulting in various ailments. The local cellular microenvironment enriched with secretory components aids in cell-cell communication that restores this homeostasis. Deciphering the underlying mechanism behind this restorative potential of secretome could serve as a possible solution to many health hazards. We, therefore, explored the protective efficacy of the secretome of differentiated PC12 cells with emphasis on induction of autophagy and mitochondrial biogenesis...
March 30, 2018: Neuromolecular Medicine
https://www.readbyqxmd.com/read/29596586/a-new-strategy-to-decrease-cardiac-injury-in-aged-heart-following-ischemia-reperfusion-enhancement-of-the-interaction-between-ampk-and-sirt1
#16
Qun Chen, Edward J Lesnefsky
No abstract text is available yet for this article.
March 27, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29590132/glp-1-receptor-agonist-ameliorates-obesity-induced-chronic-kidney-injury-via-restoring-renal-metabolism-homeostasis
#17
Chengshi Wang, Ling Li, Shuyun Liu, Guangneng Liao, Lan Li, Younan Chen, Jingqiu Cheng, Yanrong Lu, Jingping Liu
Increasing evidence indicates that obesity is highly associated with chronic kidney disease (CKD). GLP-1 receptor (GLP-1R) agonist has shown benefits on kidney diseases, but its direct role on kidney metabolism in obesity is still not clear. This study aims to investigate the protection and metabolic modulation role of liraglutide (Lira) on kidney of obesity. Rats were induced obese by high-fat diet (HFD), and renal function and metabolism changes were evaluated by metabolomic, biological and histological methods...
2018: PloS One
https://www.readbyqxmd.com/read/29574225/dioscin-alleviates-non-alcoholic-fatty-liver-disease-through-adjusting-lipid-metabolism-via-sirt1-ampk-signaling-pathway
#18
Hong Yao, Xufeng Tao, Lina Xu, Yan Qi, Lianhong Yin, Xu Han, Youwei Xu, Lingli Zheng, Jinyong Peng
Dioscin, one natural product, has active effect against non-alcoholic fatty liver disease (NAFLD) in our previous work. However, the pharmacological data are insufficient and the mechanisms have not been reported. Thus, this study aims to comprehensively investigate the effects and molecular mechanisms of dioscin against NAFLD. The primary cultured hepatocytes, AML-12 and HepG-2 cells were treated with palmic acid (PA) after dioscin treatment. The mice and rats were induced by high fat diet to establish the in vivo models of NAFLD...
March 21, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29554499/resveratrol-improves-glucose-uptake-in-insulin-resistant-adipocytes-via-sirt1
#19
Sifan Chen, Zhongliang Zhao, Liangru Ke, Zilun Li, Wenxue Li, Zili Zhang, Ying Zhou, Xiang Feng, Wei Zhu
Insulin resistance serves as "common soil" for promoting the development of metabolic diseases; however, the precise pathological factors leading to insulin resistance are not well clarified. Resveratrol (Res) is a natural polyphenolic compound with anti-inflammatory and antioxidative effects. However, effects and mechanisms of Res on glucose metabolism in adipocytes remain largely unknown. In this study, we show Res treatment significantly increases glucose uptake in insulin-resistant 3T3-L1 adipocytes in vitro...
May 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29552768/the-expression-and-mechanism-of-sirt1-and-ampk-in-nonsmall-cell-lung-cancer
#20
Fushen Yang
PURPOSE: To investigate the expression of sirtuin type 1 (Sirt1) and adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) in non-small cell lung cancer (NSCLC), and to explore the mechanism of their functions in NSCLC. METHODS: Cancer tissues (NSCLC group) and the adjacent normal healthy tissues (control group) of 50 patients with NSCLC were selected, and H&E staining was performed to identify the histopathological differences. Reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence staining and Western blot were performed to detect the expression of Sirt1 and AMPK in tissues of NSCLC group and control group...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
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