keyword
MENU ▼
Read by QxMD icon Read
search

Pythium

keyword
https://www.readbyqxmd.com/read/28542438/chemically-induced-disseminated-pythiosis-in-balb-c-mice-a-new-experimental-model-for-pythium-insidiosum-infection
#1
Juliana S M Tondolo, Érico S Loreto, Pauline C Ledur, Francielli P K Jesus, Taiara M Silva, Glaucia D Kommers, Sydney H Alves, Janio M Santurio
Pythiosis is a severe and life-threatening disease that affects humans and various animal species. We report a model of vascular/disseminated pythiosis occurring after subcutaneous inoculation of 2 x 104 Pythium insidiosum zoospores/mL in immunocompromised BALB/c mice. For this model, we carried out two rounds of experiments. First, we evaluated two protocols of immunosuppression before inoculation: cyclophosphamide at 150 mg/kg (CYP group) and cyclophosphamide 200 mg/kg plus hydrocortisone acetate at 250 mg/kg (CYP+HCA group)...
2017: PloS One
https://www.readbyqxmd.com/read/28535608/antifungal-activities-of-dimeric-sesquiterpenes-shizukaols-c-and-f-isolated-from-chloranthus-japonicus-sieb
#2
Tae Hoon Kang, Yun Mi Lee, Won Jung Lee, Eui Il Hwang, Ki Duk Park, Gyang Ja Choi, Jae Sun Moon, Ho-Yong Park, Sung Uk Kim
Two dimeric sesquitepenes were separated from Chloranthus japonicus Sieb. and identified as shizukaols C and F. They exhibited potent antifungal activities (MICs = 4-16 μg/ml) in vitro against various plant pathogenic fungi Pythium ultimum, Phytophthora infestans, Botrytis cinerea, Colletotrichum lagenarium, Alternaria kikuchana, and Magnaporthe grisea. Shizukaol C showed 88% and 91% protective activities in the greenhouse against Puccinia recondite (wheat leaf rust) and Phytophthora infestans (tomato late blight), respectively, at 100 µg/ml; shizukaol F exhibited 93% antifungal activity against Puccinia recondite at the same concentration...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28525598/differential-diagnosis-for-pythiosis-using-thermophilic-helicase-dna-amplification-and-restriction-fragment-length-polymorphism-thda-rflp
#3
Navaporn Worasilchai, Piyasak Chaumpluk, Arunaloke Chakrabarti, Ariya Chindamporn
Pythiosis is caused by Pythium insidiosum, a fungus-like microbe belonging to the kingdom Stramenopila. Its diagnosis is challenging due to clinical and histopathological similarities with the fungal microbes that cause mucormycosis and entomophthoramycosis. In addition, the proper identification of P. insidiosum in the clinical laboratory is difficult. We have developed a rapid and accurate, species-specific identification method using a thermophilic helicase DNA amplification (tHDA) technique, to differentiate this pathogen from closely related pathogenic fungi...
May 18, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28467445/oils-extracted-from-eupatorium-adenophorum-leaves-show-potential-to-control-phythium-myriotylum-in-commercially-grown-ginger
#4
Xiaoman Liu, Dongdong Yan, Canbin Ouyang, Dongsheng Yang, Qiuxia Wang, Yuan Li, Meixia Guo, Aocheng Cao
Oils extracted from the leaves of Eupatorium adenophorum were tested in vitro and in vivo against the soilborne pathogen Pythium myriotylum which causes soft rot, a devastating disease of commercial ginger production in China. Twelve compounds accounting for 99.15% of the total oil composition were identified by GC-MS. The major components were 10Hβ-9-oxo-agerophorone (37.03%), 10Hα-9-oxo-agerophorone (37.73%) and 9-oxo-10, 11-dehydro-agerophorone (23.41%). Antifungal activity was tested by the poisoned food technique against P...
2017: PloS One
https://www.readbyqxmd.com/read/28465679/comparative-transcriptome-analysis-reveals-a-preformed-defense-system-in-apple-root-of-a-resistant-genotype-of-g-935-in-the-absence-of-pathogen
#5
Yanmin Zhu, Jonathan Shao, Zhe Zhou, Robert E Davis
Two apple rootstock genotypes G.935 and B.9 were recently demonstrated to exhibit distinct resistance responses following infection by Pythium ultimum. As part of an effort to elucidate the genetic regulation of apple root resistance to soilborne pathogens, preinoculation transcriptome variations in roots of these two apple rootstock genotypes are hypothesized to contribute to the observed disease resistance phenotypes. Results from current comparative transcriptome analysis demonstrated elevated transcript abundance for many genes which function in a system-wide defense response in the root tissue of the resistant genotype of G...
2017: International Journal of Plant Genomics
https://www.readbyqxmd.com/read/28440700/selection-and-assessment-of-plant-growth-promoting-rhizobacteria-pgpr-for-biological-control-of-multiple-plant-diseases
#6
Ke Liu, Molli Newman, John A McInroy, Chia-Hui Hu, Joseph W Kloepper
A study was designed to screen individual strains of plant growth-promoting rhizobacteria (PGPR) for broad-spectrum disease suppression in vitro and in planta. In a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. In a secondary screen, these 28 strains showed broad spectrum antagonistic activity to six different genera of pathogens, and 24 of the 28 strains produced five traits reported to be related to plant growth promotion, including nitrogen fixation, phosphate solubilization, indole-3-acetic acid (IAA) production, siderophore production, and biofilm formation...
April 25, 2017: Phytopathology
https://www.readbyqxmd.com/read/28435354/control-efficacy-of-streptomyces-sp-a501-against-ginseng-damping-off-and-its-antifungal-substance
#7
Nguyen Van Minh, E-Eum Woo, Gang-Seon Lee, Dae-Won Ki, In-Kyoung Lee, Sang-Yeob Lee, Kyeonghun Park, Jaekyeong Song, Jae Eul Choi, Bong-Sik Yun
Ginseng damping-off, caused by the fungal pathogens Rhizoctonia solani and Pythium sp., is a critical disease in ginseng seedling. In a continuing effort to find microorganisms with the potential of acting as a biocontrol agent against Rhizoctonia damping-off, we found that a Streptomyces sp. A501 showed significant antifungal activity against Rhizoctonia solani. In field experiment to test the efficacy of Streptomyces sp. A501 in controlling ginseng damping-off, the incidence of damping-off disease was meaningfully reduced when ginseng seeds were soaked in the culture broth of Streptomyces sp...
March 2017: Mycobiology
https://www.readbyqxmd.com/read/28424714/relationships-between-root-pathogen-resistance-abundance-and-expression-of-pseudomonas-antimicrobial-genes-and-soil-properties-in-representative-swiss-agricultural-soils
#8
Nicola Imperiali, Francesca Dennert, Jana Schneider, Titouan Laessle, Christelle Velatta, Marie Fesselet, Michele Wyler, Fabio Mascher, Olga Mavrodi, Dmitri Mavrodi, Monika Maurhofer, Christoph Keel
Strains of Pseudomonas that produce antimicrobial metabolites and control soilborne plant diseases have often been isolated from soils defined as disease-suppressive, i.e., soils, in which specific plant pathogens are present, but plants show no or reduced disease symptoms. Moreover, it is assumed that pseudomonads producing antimicrobial compounds such as 2,4-diacetylphloroglucinol (DAPG) or phenazines (PHZ) contribute to the specific disease resistance of suppressive soils. However, pseudomonads producing antimicrobial metabolites are also present in soils that are conducive to disease...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28393124/a-leishmania-murine-model-to-evaluate-the-immunomodulatory-properties-of-pythium-insidiosum-proteins
#9
Tatiana Maria Inêz-Ferreira, Leonel Mendoza, Raquel Vilela, Hélida Monteiro de Andrade, João Paulo Haddad, Fernanda Freire Campos Nunes, Isabela Moreira Gondim, Tânia Mara Pinto Dabés Guimarães, Vicente de Paulo Coelho Peixoto de Toledo
Pythium insidiosum immunomodulatory vaccine (PiV) has been tested in clinical and experimental pythiosis. Previous data showed that P. insidiosum immunogens have the ability to switch the Th2 immune response, normally in place during pythiosis, to a curative Th1 response. Pythiosis cannot be reproduced in experimental rodents with the exception of rabbits, and thus thorough evaluation of PiV´s immunomodulatory properties has been limited by the lack of a compatible inbred mouse model. In this study, we took advantage of the murine BALB/c Leishmania infection model, where infected mice produce a Th2 response, to evaluate the PiV Th2 to Th1 immunomodulatory potential...
March 2017: Heliyon
https://www.readbyqxmd.com/read/28386770/probability-models-based-on-soil-properties-for-predicting-presence-absence-of-pythium-in-soybean-roots
#10
Kimberly K Zitnick-Anderson, Jack E Norland, Luis E Del Río Mendoza, Ann-Marie Fortuna, Berlin D Nelson
Associations between soil properties and Pythium groups on soybean roots were investigated in 83 commercial soybean fields in North Dakota. A data set containing 2877 isolates of Pythium which included 26 known spp. and 1 unknown spp. and 13 soil properties from each field were analyzed. A Pearson correlation analysis was performed with all soil properties to observe any significant correlation between properties. Hierarchical clustering, indicator spp., and multi-response permutation procedures were used to identify groups of Pythium...
April 6, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28348550/an-in-vitro-study-of-bio-control-and-plant-growth-promotion-potential-of-salicaceae-endophytes
#11
Shyam L Kandel, Andrea Firrincieli, Pierre M Joubert, Patricia A Okubara, Natalie D Leston, Kendra M McGeorge, Giuseppe S Mugnozza, Antoine Harfouche, Soo-Hyung Kim, Sharon L Doty
Microbial communities in the endosphere of Salicaceae plants, poplar (Populus trichocarpa) and willow (Salix sitchensis), have been demonstrated to be important for plant growth promotion, protection from biotic and abiotic stresses, and degradation of toxic compounds. Our study aimed to investigate bio-control activities of Salicaceae endophytes against various soil borne plant pathogens including Rhizoctonia solani AG-8, Fusarium culmorum, Gaeumannomyces graminis var. tritici, and Pythium ultimum. Additionally, different plant growth promoting traits such as biological nitrogen fixation (BNF), indole-3-acetic acid (IAA) biosynthesis, phosphate solubilization, and siderophore production were assessed in all bio-control positive strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28346370/de-novo-assembly-annotation-and-characterization-of-root-transcriptomes-of-three-caladium-cultivars-with-a-focus-on-necrotrophic-pathogen-resistance-defense-related-genes
#12
Zhe Cao, Zhanao Deng
Roots are vital to plant survival and crop yield, yet few efforts have been made to characterize the expressed genes in the roots of non-model plants (root transcriptomes). This study was conducted to sequence, assemble, annotate, and characterize the root transcriptomes of three caladium cultivars (Caladium × hortulanum) using RNA-Seq. The caladium cultivars used in this study have different levels of resistance to Pythiummyriotylum, the most damaging necrotrophic pathogen to caladium roots. Forty-six to 61 million clean reads were obtained for each caladium root transcriptome...
March 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28336598/draft-genome-sequence-of-the-mycoparasitic-oomycete-pythium-periplocum-strain-cbs-532-74
#13
Sandeep K Kushwaha, Ramesh R Vetukuri, Laura J Grenville-Briggs
The oomycete Pythium periplocum is an aggressive mycoparasite of a number of plant pathogenic fungi and oomycetes and therefore has potential as a biological control agent. Here, we report the first draft genome sequence of P. periplocum, which comprises 35.89 Mb. It contains 1,043 scaffolds and 14,399 predicted protein-coding genes.
March 23, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28303127/functional-characterization-of-endophytic-fungal-community-associated-with-oryza-sativa-l-and-zea-mays-l
#14
Momota Potshangbam, S Indira Devi, Dinabandhu Sahoo, Gary A Strobel
In a natural ecosystem, the plant is in a symbiotic relationship with beneficial endophytes contributing huge impact on its host plant. Therefore, exploring beneficial endophytes and understanding its interaction is a prospective area of research. The present work aims to characterize the fungal endophytic communities associated with healthy maize and rice plants and to study the deterministic factors influencing plant growth and biocontrol properties against phytopathogens, viz, Pythium ultimum, Sclerotium oryzae, Rhizoctonia solani, and Pyricularia oryzae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28217886/the-use-of-newly-isolated-streptomyces-strain-tn258-as-potential-biocontrol-agent-of-potato-tubers-leak-caused-by-pythium-ultimum
#15
Imen Sellem, Mohamed Ali Triki, Lobna Elleuch, Manel Cheffi, Ahlem Chakchouk, Slim Smaoui, Lotfi Mellouli
An actinomycete strain designated TN258, was isolated from Tunisian Sahara soil and selected for its antagonistic activity, especially against Pythium ultimum (P. ultimum) causing potato tubers leak. Based on the results of cultural characteristic of TN258 strain, the 16S rRNA gene nucleotide sequence (1433 bp, accession n° HE600071) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Streptomyces TN258 strain. After culture optimization, the inhibitory effect of TN258 free cell supernatant against P...
February 20, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28207431/successful-medical-management-of-presumptive-pythium-insidiosum-keratitis
#16
Muralidhar Ramappa, Ritu Nagpal, Savitri Sharma, Sunita Chaurasia
PURPOSE: To describe the previously unreported successful treatment of presumptive Pythium keratitis (PK) with medical therapy alone. METHODS: A 42-year-old female homemaker presented to us with a 15-day history of pain and redness in the right eye after a trivial injury. Her vision was 20/80 at presentation. Slit-lamp biomicroscopy revealed a central, dense and dry-looking, grayish-white infiltrate reaching mid stroma. The infiltrate had feathery margins and was surrounded by multiple tentacle-like lesions and peripherally expanding pinhead-sized subepithelial lesions...
February 15, 2017: Cornea
https://www.readbyqxmd.com/read/28188331/urnula-sp-an-endophyte-of-dicksonia-antarctica-making-a-fragrant-mixture-of-biologically-active-volatile-organic-compounds
#17
Gary Strobel, Amy Ericksen, Joe Sears, Jie Xie, Brad Geary, Bryan Blatt
Urnula sp. was isolated as an endophyte of Dicksonia antarctica and identified primarily on the basis of its ITS sequence and morphological features. The anamorphic state of the fungus appeared as a hyphomyceteous-like fungus as based on its features in culture and scanning electron microscopy examination of its spores. On potato dextrose agar (PDA), the organism makes a characteristic fragrance resembling peach pie with vanilla overtones. A GC/MS analysis done on the volatile organic compounds (VOCs) of this organism, trapped by carbotrap methodology, revealed over 150 compounds with high MS matching quality being noted for 44 of these...
February 10, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28178423/physiological-and-molecular-characterization-of-compost-bacteria-antagonistic-to-soil-borne-plant-pathogens
#18
Rowida Mohamed, Emma Groulx, Stefanie Defilippi, Tamara Erak, James T Tambong, Russell J Tweddell, Apollinaire Tsopmo, Tyler J Avis
Disease suppressive composts have the potential to mitigate the risks associated with chemical pesticides. One of the main characteristics responsible for the suppressive nature of composts is their microbiological populations. To gain insight into the determinants responsible for their suppressive effects, we assayed composts to (i) isolate and identify beneficial antagonistic bacteria, (ii) quantify their antifungal and anti-oomycetal activities, (iii) extract inhibitory compounds produced by the bacteria, and (iv) identify antimicrobial lipopeptides produced by these bacteria...
February 8, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28115356/evolution-of-the-sterol-biosynthetic-pathway-of-pythium-insidiosum-and-related-oomycetes-contributes-to-antifungal-drug-resistance
#19
Tassanee Lerksuthirat, Areeporn Sangcakul, Tassanee Lohnoo, Wanta Yingyong, Thidarat Rujirawat, Theerapong Krajaejun
Pythiosis is a life-threatening infectious disease caused by the oomycete Pythium insidiosum Direct exposure to Py. insidiosum zoospores can initiate infections of the eye, limb, gastrointestinal tract, or skin/subcutaneous tissue. Treatments for pythiosis have mostly relied on surgery. Antifungal drugs are generally ineffective against Py. insidiosum However, one patient with an invasive Py. insidiosum infection recovered completely following treatment with terbinafine and itraconazole. Additionally, the drug target sterol biosynthetic enzymes have been identified in the oomycete Aphanomyces euteiches It remains an open question whether Py...
April 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28050341/cutaneous-pythiosis-in-calves-an-epidemiologic-pathologic-serologic-and-molecular-characterization
#20
Guilherme Konradt, Daniele Mariath Bassuino, Matheus Viezzer Bianchi, Lismara Castro, Rafaela Albuquerque Caprioli, Saulo Petinatti Pavarini, Janio M Santurio, Maria Isabel Azevedo, Francielli P Jesus, David Driemeier
This study reports the epidemiological, pathological and mycological findings of cutaneous pythiosis in cattle in southern Brazil. 23 calves, that were kept next to a river with extensive marshy regions, presented ulcerated cutaneous lesions in thoracic and pelvic limbs, sometimes extending to the ventral thoracic region. Histopathological examination revealed multifocal pyogranulomas in the superficial and deep dermis. The Grocott-Methenamine silver, immunohistochemistry anti-Pythium insidiosum, ELISA serology and molecular characterization demonstrated the agent P...
December 2016: Medical Mycology Case Reports
keyword
keyword
92096
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"