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https://www.readbyqxmd.com/read/29319595/pythium-keratitis-leading-to-fatal-cavernous-sinus-thrombophlebitis
#1
Anubha Rathi, Arunaloke Chakrabarti, Tushar Agarwal, Neelam Pushker, Mukesh Patil, Hemant Kamble, Jeewan S Titiyal, Rishi Mohan, Seema Kashyap, Sanjay Sharma, Seema Sen, Gita Satpathy, Namrata Sharma
PURPOSE: To report a case of Pythium insidiosum keratitis leading to fatal cavernous sinus thrombophlebitis. METHODS: Case report. RESULTS: A 70-year-old man presented with excruciating pain, redness, and diminution of vision in his left eye for 2 weeks after washing his hair with tap water. A total corneal ulcer with surrounding infiltrates and associated corneal thinning was present. Corneal scraping revealed the presence of Gram-positive cocci...
January 9, 2018: Cornea
https://www.readbyqxmd.com/read/29311087/in-vitro-activities-of-miltefosine-and-antibacterial-agents-from-macrolide-oxazolidinone-and-pleuromutilin-classes-against-pythium-insidiosum-and-pythium-aphanidermatum
#2
Erico S Loreto, Juliana S M Tondolo, Daniele C Oliveira, Janio M Santurio, Sydney H Alves
We tested 29 isolates of Pythium insidiosum and one isolate of Pythium aphanidermatum to investigate their susceptibility to miltefosine and antibacterial drugs from macrolide, oxazolidinone, and pleuromutilin classes. We found that miltefosine, azithromycin, clarithromycin, josamycin, linezolid, sutezolid, retapamulin, tiamulin, and valnemulin had inhibitory and cidal activity against the pathogens at concentrations ranging from 0.25 to 64 μg/ml. Our results suggest that these antimicrobials are promising candidates for future studies on pythiosis in animals and humans...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29232885/design-synthesis-and-fungicidal-activity-of-novel-thiosemicarbazide-derivatives-containing-piperidine-fragments
#3
Xuebo Zhang, Peng Lei, Tengda Sun, Xiaoyu Jin, Xinling Yang, Yun Ling
In order to discover novel eco-friendly lead compounds for plant pathogenic fungi control, a series of benzaldehyde thiosemicarbazide derivatives with a piperidine moiety have been designed and synthesized. Fungicidal activities of all the synthesized compounds were evaluated in vitro. The results indicated that all the title compounds exhibited moderate to good fungicidal activities. Compound 3b displayed excellent activities against Pythium aphanidermatum, Rhizoctonia solani, Valsa mali, and Gaeu-mannomyces graminsis, with EC50 values lower than 10 μg/mL...
December 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29228389/high-resolution-melting-analysis-a-novel-approach-for-clade-differentiation-in-pythium-insidiosum-and-pythiosis
#4
Navaporn Worasilchai, Nitipong Permpalung, Ariya Chindamporn
Pythium insidiosum causes life-threatening human pythiosis. Based on phylogenetic analysis using internal transcribed spacer (ITS) region, mitochondrial cytochrome C oxidase II (COX2) gene, intergenic spacer (IGS) region and exo-1,3-β-glucanase gene (exo1), P. insidiosum is classified into clade ATH, BTH, and CTH related to geographic distribution. At present, polymerase chain reaction in any of these specific regions with DNA sequencing is the only technique to provide clade diagnosis. In this study, P. insidiosum-specific primers targeting COX2 gene were designed and used in real-time quantitative polymerase chain reaction (qPCR) with subsequent high-resolution melting (HRM) to provide rapid identification as well as clade classification for P...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29228375/influence-of-temperature-on-in-vitro-zoosporogenesis-of-pythium-insidiosum
#5
Cristina Gomes Zambrano, Angelita Reis Gomes, Carolina Litchina Brasil, Júlia de Souza Silveira Valente, Carolina Quintana Braga, Maria Isabel de Azevedo, Sônia de Avila Botton, Daniela Isabel Brayer Pereira
This study verified the influence of different temperatures on P. insidiosum in vitro zoosporogenesis. P. insidiosum isolates (n = 26) were submitted to zoosporogenesis and incubated at 5°C, 15°C, 20°C and 37°C (1st stage). Grass fragments were evaluated under optical microscopy at 4, 8, and 24 hours of incubation. Afterward, all isolates were incubated at 37°C and assessed at the same periods of time (2nd stage). The development of hyphae, presence of vesicles, zoosporangia and zoospores were checked...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29202039/genomic-network-and-phylogenetic-analysis-of-the-oomycete-effector-arsenal
#6
Jamie McGowan, David A Fitzpatrick
The oomycetes are a class of microscopic, filamentous eukaryotes within the stramenopiles-alveolate-Rhizaria (SAR) supergroup and include ecologically significant animal and plant pathogens. Oomycetes secrete large arsenals of effector proteins that degrade host cell components, manipulate host immune responses, and induce necrosis, enabling parasitic colonization. This study investigated the expansion and evolution of effectors in 37 oomycete species in 4 oomycete orders, including Albuginales, Peronosporales, Pythiales, and Saprolegniales species...
November 2017: MSphere
https://www.readbyqxmd.com/read/29184544/modeling-effects-of-temperature-soil-moisture-nutrition-and-variety-as-determinants-of-severity-of-pythium-damping-off-and-root-disease-in-subterranean-clover
#7
Ming P You, Kelly Rensing, Michael Renton, Martin J Barbetti
Subterranean clover (Trifolium subterraneum) is a critical pasture legume in Mediterranean regions of southern Australia and elsewhere, including Mediterranean-type climatic regions in Africa, Asia, Australia, Europe, North America, and South America. Pythium damping-off and root disease caused by Pythium irregulare is a significant threat to subterranean clover in Australia and a study was conducted to define how environmental factors (viz. temperature, soil type, moisture and nutrition) as well as variety, influence the extent of damping-off and root disease as well as subterranean clover productivity under challenge by this pathogen...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29177752/bacterial-shifts-in-nutrient-solutions-flowing-through-biofilters-used-in-tomato-soilless-culture
#8
David Renault, Franck Déniel, Jessica Vallance, Emilie Bruez, Jean-Jacques Godon, Patrice Rey
In soilless culture, slow filtration is used to eliminate plant pathogenic microorganisms from nutrient solutions. The present study focused on the characterization and the potential functions of microbial communities colonizing the nutrient solutions recycled on slow filters during a whole cultivation season of 7 months in a tomato growing system. Bacterial microflora colonizing the solutions before and after they flew through the columns were studied. Two filters were amended with Pseudomonas putida (P-filter) or Bacillus cereus strains (B-filter), and a third filter was a control (C-filter)...
November 25, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/29176690/fungal-endophytes-inhabiting-mountain-cultivated-ginseng-panax-ginseng-meyer-diversity-and-biocontrol-activity-against-ginseng-pathogens
#9
Young-Hwan Park, Yoosam Kim, Ratnesh Chandra Mishra, Hanhong Bae
Fungal endophytes isolated from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) were explored for their diversity and biocontrol activity against ginseng pathogens (Alternaria panax, Botrytis cinerea, Cylindrocarpon destructans, Pythium sp. and Rhizoctonia solani). A total of 1,300 isolates were isolated from three tissues (root, stem and leaf) from MCGs grown in 24 different geographic locations in Korea. In total, 129 different fungal isolates were authenticated by molecular identification based on internal transcribed spacer (ITS) sequences...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29176454/pythium-insidiosum-keratitis-histopathology-and-rapid-novel-diagnostic-staining-technique
#10
Paavan Kalra, Bhupesh Bagga, Prashant Garg
No abstract text is available yet for this article.
November 22, 2017: Cornea
https://www.readbyqxmd.com/read/29176453/role-of-confocal-microscopy-in-the-diagnosis-of-pythium-insidiosum-keratitis
#11
Orapin Anutarapongpan, Onsiri Thanathanee, Jittraporn Worrawitchawong, Olan Suwan-Apichon
PURPOSE: To investigate the role of in vivo confocal microscopy for detection of Pythium insidiosum keratitis. METHODS: Medical records and confocal microscopy findings of 20 patients (21 eyes) with culture- or polymerase chain reaction-proven P. insidiosum keratitis diagnosed at the Khon Kaen University Eye Center from January 2009 to December 2015 were retrospectively reviewed. Confocal microscopy was performed using Nidek ConfoScan 4. The images from 21 eyes with P...
November 22, 2017: Cornea
https://www.readbyqxmd.com/read/29166533/potential-effects-of-fungicide-and-algaecide-extracts-of-annona-glabra-l-annonaceae-on-the-microalgae-raphidocelis-subcapitata-and-on-the-oomycete-pythium
#12
Giseli S Rocha, Reginaldo S Matsumoto, Ana Teresa Lombardi, Maria Inês S Lima
Annona glabra L. is a semi-deciduous tree that contains several active substances, including secondary metabolites, with antifungal activity. Phytopathogenic strains of the genus Pythium cause billion dollar losses all over the world on natural and crop species. Searching for eco-friendly algaecides and fungicides, we analyzed the effects of acetone extracts of A. glabra leaves on the algae Rhaphidocelis subcapitata (Korshikov) and on the oomycete Pythium aphanidermatum (Edson). We evaluated ten extract concentrations for each organism - 0 to 400 mg L-1 for algae and 0-1000 µg disc1 for oomycete...
2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29159227/draft-genome-and-sequence-variant-data-of-the-oomycete-pythium-insidiosum-strain-pi45-from-the-phylogenetically-distinct-clade-iii
#13
Weerayuth Kittichotirat, Preecha Patumcharoenpol, Thidarat Rujirawat, Tassanee Lohnoo, Wanta Yingyong, Theerapong Krajaejun
Pythium insidiosum is a unique oomycete microorganism, capable of infecting humans and animals. The organism can be phylogenetically categorized into three distinct clades: Clade-I (strains from the Americas); Clade-II (strains from Asia and Australia), and Clade-III (strains from Thailand and the United States). Two draft genomes of the P. insidiosum Clade-I strain CDC-B5653 and Clade-II strain Pi-S are available in the public domain. The genome of P. insidiosum from the distinct Clade-III, which is distantly-related to the other two clades, is lacking...
December 2017: Data in Brief
https://www.readbyqxmd.com/read/29127333/a-light-activated-antimicrobial-surface-is-active-against-bacterial-viral-and-fungal-organisms
#14
Tim Walker, Melisa Canales, Sacha Noimark, Kristopher Page, Ivan Parkin, Jane Faull, Manni Bhatti, Lena Ciric
Evidence has shown that environmental surfaces play an important role in the transmission of nosocomial pathogens. Deploying antimicrobial surfaces in hospital wards could reduce the role environmental surfaces play as reservoirs for pathogens. Herein we show a significant reduction in viable counts of Staphylococcus epidermidis, Saccharomyces cerevisiae, and MS2 Bacteriophage after light treatment of a medical grade silicone incorporating crystal violet, methylene blue and 2 nm gold nanoparticles. Furthermore, a migration assay demonstrated that in the presence of light, growth of the fungus-like organism Pythium ultimum and the filamentous fungus Botrytis cinerea was inhibited...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29111192/antifungal-molluscicidal-and-larvicidal-assessment-of-anemonin-and-clematis-flammula-l-extracts-against-mollusc-galba-truncatula-intermediate-host-of-fasciola-hepatica-in-tunisia
#15
Rakia Saidi, Lamia Khanous, Safa Khadim Allah, Besma Hamdi, Ali Ayadi, Mohamed Damak, Hayet Hammami, Raoudha Mezghani-Jarraya
OBJECTIVE: To investigate the potential of anemonin and Clematis flammula (C. flammula) extracts against infective organisms. METHODS: The molluscicidal activities of anemonin and C. flammula extracts against Galba truncatula Müll. (Lymnaeidae) and Fasciola hepatica larval stages contaminating this snail in Tunisia were assessed by testing six groups of snails in 250 mL of extracts and aqueous dechlorinated solutions with different concentrations (ranging from 2...
October 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29101453/endophytic-paraconiothyrium-sp-from-zingiber-officinale-rosc-displays-broad-spectrum-antimicrobial-activity-by-production-of-danthron
#16
C Anisha, P Sachidanandan, E K Radhakrishnan
The bioactivity spectrum of fungal endophytes isolated from Zingiber officinale was analyzed against clinical pathogens and against the phytopathogen Pythium myriotylum, which causes Pythium rot in ginger. One of the isolates GFM13 showed broad bioactivity against various pathogens tested including P. myriotylum. The spore suspension as well as the culture filtrate of the endophytic fungal isolate was found to effectively protect ginger rhizomes from Pythium rot. By molecular identification, the fungal endophyte was identified as Paraconiothyrium sp...
November 3, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29095755/azithromycin-in-the-management-of-pythium-insidiosum-keratitis
#17
Samrat Chatterjee, Deepshikha Agrawal
No abstract text is available yet for this article.
October 31, 2017: Cornea
https://www.readbyqxmd.com/read/29074300/dendritic-cells-pulsed-with-pythium-insidiosum-1-3-1-6-%C3%AE-glucan-heat-inactivated-zoospores-and-immunotherapy-prime-na%C3%A3-ve-t-cells-to-th1-differentiation-in-vitro
#18
Pauline C Ledur, Juliana S M Tondolo, Francielli P K Jesus, Camila M Verdi, Érico S Loreto, Sydney H Alves, Janio M Santurio
Pythiosis is a life-threatening disease caused by the fungus-like microorganism Pythium insidiosum that can lead to death if not treated. Since P. insidiosum has particular cell wall characteristics, pythiosis is difficult to treat, as it does not respond well to traditional antifungal drugs. In our study, we investigated a new immunotherapeutic approach with potential use in treatment and in the acquisition of immunity against pythiosis. Dendritic cells from both human and mouse, pulsed with P. insidiosum heat-inactivated zoospore, (1,3)(1,6)-β-glucan and the immunotherapeutic PitiumVac(®) efficiently induced naïve T cell differentiation in a Th1 phenotype by the activation of specific Th1 cytokine production in vitro...
October 18, 2017: Immunobiology
https://www.readbyqxmd.com/read/29017458/lifestyle-gene-gain-and-loss-and-transcriptional-remodeling-cause-divergence-in-the-transcriptomes-of-phytophthora-infestans-and-pythium-ultimum-during-potato-tuber-colonization
#19
Audrey M V Ah-Fong, Jolly Shrivastava, Howard S Judelson
BACKGROUND: How pathogen genomes evolve to support distinct lifestyles is not well-understood. The oomycete Phytophthora infestans, the potato blight agent, is a largely biotrophic pathogen that feeds from living host cells, which become necrotic only late in infection. The related oomycete Pythium ultimum grows saprophytically in soil and as a necrotroph in plants, causing massive tissue destruction. To learn what distinguishes their lifestyles, we compared their gene contents and expression patterns in media and a shared host, potato tuber...
October 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28972292/embryonated-chicken-eggs-an-experimental-model-for-pythium-insidiosum-infection
#20
Camila M Verdi, Francielli P K Jesus, Glaucia Kommers, Pauline C Ledur, Maria I Azevedo, Erico S Loreto, Juliana S M Tondolo, Eduardo N C Andrade, Karine B Schlemmer, Sydney H Alves, Janio M Santurio
Pythiosis is a severe disease caused by Pythium insidiosum. Currently, the research on the treatment of pythiosis uses rabbits as an experimental infection model. To reduce the use of animals in scientific experimentation, alternative models are increasingly necessary options. The objective of this study was to establish a new experimental infection model for pythiosis using embryonated chicken eggs. First, we tested the inoculation of 4 zoospore concentrations into the egg allantoic cavity at 3 embryonic days...
October 3, 2017: Mycoses
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