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https://www.readbyqxmd.com/read/28690628/comparison-of-salt-tolerance-in-soja-based-on-metabolomics-of-seedling-roots
#1
Mingxia Li, Rui Guo, Yang Jiao, Xiaofei Jin, Haiyan Zhang, Lianxuan Shi
Soybean is an important economic crop that is continually threatened by abiotic stresses, especially salt stress. Wild soybean is an important germplasm resource for the breeding of cultivated soybean. The root system plays a very important role in plant salt tolerance. To explore the salt tolerance-related mechanisms among Soja, we have demonstrated the seedling roots' growth and metabolomics in wild soybean, semi-wild soybean, and cultivated soybean under two types of salt stress by using gas chromatography-mass spectrometry...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28662355/metabolic-engineering-pathways-for-rare-sugars-biosynthesis-physiological-functionalities-and-applications-a-review
#2
Muhammad Bilal, Hafiz M N Iqbal, Hongbo Hu, Wei Wang, Xuehong Zhang
Biomolecules like rare sugars and their derivatives are referred to as monosaccharides particularly uncommon in nature. Remarkably, many of them have various known physiological functions and biotechnological applications in cosmetics, nutrition and pharmaceutical industries. Also, they can be exploited as starting materials for synthesizing fascinating natural bioproducts with significant biological activities. Regrettably, most of the rare sugars are quite expensive, and their synthetic chemical routes are both limited and economically unfeasible due to expensive raw materials...
June 29, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28549946/potential-metabolomic-biomarkers-for-reliable-diagnosis-of-behcet-s-disease-using-gas-chromatography-with-time-of-flight-mass-spectrometry
#3
Joong Kyong Ahn, Jungyeon Kim, Jiwon Hwang, Juhwan Song, Kyoung Heon Kim, Hoon-Suk Cha
OBJECTIVES: Although many diagnostic criteria of Behcet's disease (BD) have been developed and revised by experts, diagnosing BD is still complicated and challenging. No metabolomic studies on serum have been attempted to improve the diagnosis and to identify potential biomarkers of BD. The purposes of this study were to investigate distinctive metabolic changes in serum samples of BD patients and to identify metabolic candidate biomarkers for reliable diagnosis of BD using the metabolomics platform...
May 23, 2017: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28530095/rational-design-of-bacillus-coagulans-nl01-l-arabinose-isomerase-and-use-of-its-f279i-variant-in-d-tagatose-production
#4
Zhaojuan Zheng, Wending Mei, Meijuan Xia, Qin He, Jia Ouyang
d-Tagatose is a prospective functional sweetener that can be produced by l-arabinose isomerase (AI) from d-galactose. To improve the activity of AI toward d-galactose, the AI of Bacillus coagulans was rationally designed on the basis of molecular modeling and docking. After alanine scanning and site-saturation mutagenesis, variant F279I that exhibited improved activity toward d-galactose was obtained. The optimal temperature and pH of F279I were determined to be 50 °C and 8.0, respectively. This variant possessed 1...
June 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28512318/structure-based-prediction-and-identification-of-4-epimerization-activity-of-phosphate-sugars-in-class-ii-aldolases
#5
Seon-Hwa Lee, Seung-Hye Hong, Jung-Ung An, Kyoung-Rok Kim, Dong-Eun Kim, Lin-Woo Kang, Deok-Kun Oh
Sugar 4-epimerization reactions are important for the production of rare sugars and their derivatives, which have various potential industrial applications. For example, the production of tagatose, a functional sweetener, from fructose by sugar 4-epimerization is currently constrained because a fructose 4-epimerase does not exist in nature. We found that class II D-fructose-1,6-bisphosphate aldolase (FbaA) catalyzed the 4-epimerization of D-fructose-6-phosphate (F6P) to D-tagatose-6-phosphate (T6P) based on the prediction via structural comparisons with epimerase and molecular docking and the identification of the condensed products of C3 sugars...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28481935/genotypic-and-phenotypic-characterization-of-multidrug-resistant-salmonella-typhimurium-and-salmonella-kentucky-strains-recovered-from-chicken-carcasses
#6
Rizwana Tasmin, Nur A Hasan, Christopher J Grim, Ar'Quette Grant, Seon Young Choi, M Samiul Alam, Rebecca Bell, Christopher Cavanaugh, Kannan V Balan, Uma S Babu, Salina Parveen
Salmonella Typhimurium is the leading cause of human non-typhoidal gastroenteritis in the US. S. Kentucky is one the most commonly recovered serovars from commercially processed poultry carcasses. This study compared the genotypic and phenotypic properties of two Salmonella enterica strains Typhimurium (ST221_31B) and Kentucky (SK222_32B) recovered from commercially processed chicken carcasses using whole genome sequencing, phenotype characterizations and an intracellular killing assay. Illumina MiSeq platform was used for sequencing of two Salmonella genomes...
2017: PloS One
https://www.readbyqxmd.com/read/28480150/biobased-furanics-kinetic-studies-on-the-acid-catalyzed-decomposition-of-2-hydroxyacetyl-furan-in-water-using-br%C3%A3-nsted-acid-catalysts
#7
J N M Soetedjo, H H van de Bovenkamp, P J Deuss, H J Heeres
Biobased furanics like 5-hydroxymethylfurfural (5-HMF) are interesting platform chemicals for the synthesis of biofuel additives and polymer precursors. 5-HMF is typically prepared from C6 ketoses like fructose, psicose, sorbose and tagatose. A known byproduct is 2-hydroxyacetylfuran (2-HAF), particularly when using sorbose and psicose as the reactants. We here report an experimental and kinetic modeling study on the rate of decomposition of 2-HAF in a typical reaction medium for 5-HMF synthesis (water, Brönsted acid), with the incentive to gain insights in the stability of 2-HAF...
May 1, 2017: ACS Sustainable Chemistry & Engineering
https://www.readbyqxmd.com/read/28458081/reactivity-studies-in-water-on-the-acid-catalysed-dehydration-of-psicose-compared-to-other-ketohexoses-into-5-hydroxymethylfurfural
#8
Robert-Jan van Putten, Jan C van der Waal, Ed de Jong, Hero J Heeres
The conversion of the four possible ketohexoses (fructose, tagatose, sorbose and psicose) into 5-hydroxymethylfurfural (HMF) was explored in water using sulphuric acid as the catalyst (33 mM H2SO4, 120 °C). Significant differences in reactivity were observed and tagatose (48% conversion after 75 min) and psicose (35% conversion after 75 min) were clearly more reactive than fructose and sorbose (around 20% conversion after 75 min). The selectivity to HMF was found to be higher for fructose and psicose than for tagatose and sorbose...
April 13, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28405835/high-yield-production-of-pure-tagatose-from-fructose-by-a-three-step-enzymatic-cascade-reaction
#9
Seon-Hwa Lee, Seung-Hye Hong, Kyoung-Rok Kim, Deok-Kun Oh
OBJECTIVE: To produce tagatose from fructose with a high conversion rate and to establish a high-yield purification method of tagatose from the reaction mixture. RESULTS: Fructose at 1 M (180 g l(-1)) was converted to 0.8 M (144 g l(-1)) tagatose by a three-step enzymatic cascade reaction, involving hexokinase, plus ATP, fructose-1,6-biphosphate aldolase, phytase, over 16 h with a productivity of 9 g l(-1) h(-1). No byproducts were detected. Tagatose was recrystallized from ethanol to a purity of 99...
April 12, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28371762/d-tagatose-production-by-permeabilized-and-immobilized-lactobacillus-plantarum-using-whey-permeate
#10
J Jayamuthunagai, G Srisowmeya, M Chakravarthy, P Gautam
The aim of the work is to produce d-Tagatose by direct addition of alginate immobilized Lactobacillus plantarum cells to lactose hydrolysed whey permeate. The cells were untreated and immobilized (UIC), permeabilized and immobilized (PIC) and the relative activities were compared with purified l-arabinose isomerase (l-AI) for d-galactose isomerization. Successive lactose hydrolysis by β-galactosidase from Escherichia coli and d-galactose isomerization using l-AI from Lactobacillus plantarum was performed to investigate the in vivo production of d-tagatose in whey permeate...
July 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28258150/tm0416-a-hyperthermophilic-promiscuous-nonphosphorylated-sugar-isomerase-catalyzes-various-c5-and-c6-epimerization-reactions
#11
Sun-Mi Shin, Thinh-Phat Cao, Jin Myung Choi, Seong-Bo Kim, Sang-Jae Lee, Sung Haeng Lee, Dong-Woo Lee
There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene in Escherichia coli and purified the resulting recombinant protein for detailed characterization. Amino acid sequence alignment and a structural similarity search revealed that TM0416 is a putative nonphosphorylated sugar epimerase...
May 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28017979/physicochemical-characteristics-of-citrus-jelly-with-non-cariogenic-and-functional-sweeteners
#12
Susana Rubio-Arraez, Juan Vicente Capella, María Luisa Castelló, María Dolores Ortolá
In this study the effect of sweeteners with low glycemic index and non-cariogenic characteristics (isomaltulose, oligofructose and tagatose) in jelly prepared with citrus juice has been evaluated considering a citrus jelly formulated with sucrose as reference. The soluble solids, moisture content, pH, water activity, antioxidant capacity, optical and mechanical properties of jelly made using different sweeteners was determined during storage. Besides, mesophilic aerobics and moulds and yeasts was also counted to determine their stability over time...
October 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28010770/a-single-and-two-step-isomerization-process-for-d-tagatose-and-l-ribose-bioproduction-using-l-arabinose-isomerase-and-d-lyxose-isomerase
#13
Manisha J Patel, Rekha C Akhani, Arti T Patel, Samir R Dedania, Darshan H Patel
l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerization using Sf-AI and d-lyxose/ribose isomerase from Cohnella laevoribosii. The overall 22.3% and 25% conversion rate were observed for d-tagatose and l-ribose production from d-galactose and l-arabinose respectively...
February 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27998065/pathway-construction-in-corynebacterium-glutamicum-and-strain-engineering-to-produce-rare-sugars-from-glycerol
#14
Jiangang Yang, Yueming Zhu, Yan Men, Shangshang Sun, Yan Zeng, Ying Zhang, Yuanxia Sun, Yanhe Ma
Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered...
December 21, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27889146/the-potential-of-species-specific-tagatose-6-phosphate-t6p-pathway-in-lactobacillus-casei-group-for-galactose-reduction-in-fermented-dairy-foods
#15
COMPARATIVE STUDY
Qinglong Wu, Nagendra P Shah
Residual lactose and galactose in fermented dairy foods leads to several industrial and health concerns. There is very little information pertaining to manufacture of fermented dairy foods that are low in lactose and galactose. In the present study, comparative genomic survey demonstrated the constant presence of chromosome-encoded tagatose-6-phosphate (T6P) pathway in Lactobacillus casei group. Lactose/galactose utilization tests and β-galactosidase assay suggest that PTS(Gal) system, PTS(Lac) system and T6P pathway are major contributors for lactose/galactose catabolism in this group of organisms...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27748561/gas-chromatography-analysis-of-major-free-mono-and-disaccharides-in-milk-method-assessment-validation-and-application-to-real-samples
#16
Ilenia Idda, Nadia Spano, Marco Ciulu, Valeria M Nurchi, Angelo Panzanelli, Maria I Pilo, Gavino Sanna
Saccharides are functional constituents of milk. Although d-lactose represents almost the totality of the saccharides in the milk, minor species, like d-glucose, d-galactose, myo-inositol and, as a result of severe thermal treatments, monosaccharides like d-tagatose, are also detectable. Although chromatography has been the main analytical approach used for accomplishing this task, quite surprisingly a validated gas chromatographic method aimed at the simultaneous determination of these compounds is still needed...
December 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27734668/increased-production-of-food-grade-d-tagatose-from-d-galactose-by-permeabilized-and-immobilized-cells-of-corynebacterium-glutamicum-a-gras-host-expressing-d-galactose-isomerase-from-geobacillus-thermodenitrificans
#17
Kyung-Chul Shin, Dong-Hyun Sim, Min-Ju Seo, Deok-Kun Oh
The generally recognized as safe microorganism Corynebacterium glutamicum expressing Geobacillus thermodenitrificans d-galactose isomerase (d-GaI) was an efficient host for the production of d-tagatose, a functional sweetener. The d-tagatose production at 500 g/L d-galactose by the host was 1.4-fold higher than that by Escherichia coli expressing d-GaI. The d-tagatose-producing activity of permeabilized C. glutamicum (PCG) cells treated with 1% (w/v) Triton X-100 was 2.1-fold higher than that of untreated cells...
November 2, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27699891/sweet-structural-signatures-unveiled-in-ketohexoses
#18
Celina Bermúdez, Isabel Peña, Santiago Mata, José L Alonso
The conformational behaviour of naturally occurring ketohexoses has been revealed in a supersonic expansion by Fourier transform microwave spectroscopy coupled with a laser ablation source. Three, two and one conformers of d-tagatose, d-psicose and l-sorbose, respectively, have been identified by their rotational constants extracted from the analysis of the spectra. Singular structural signatures involving the hydroxyl groups OH(1) and OH(2) have been disentangled from the intricate intramolecular hydrogen bond networks stabilising the most abundant conformers...
November 14, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27586234/cloning-expression-and-characterization-of-a-novel-l-arabinose-isomerase-from-the-psychrotolerant-bacterium-pseudoalteromonas-haloplanktis
#19
Wei Xu, Chen Fan, Tao Zhang, Bo Jiang, Wanmeng Mu
L-Arabinose isomerase (L-AI, EC 5.3.1.4) catalyzes the isomerization between L-arabinose and L-ribulose, and most of the reported ones can also catalyze D-galactose to D-tagatose, except Bacillus subtilis L-AI. In this article, the L-AI from the psychrotolerant bacterium Pseudoalteromonas haloplanktis ATCC 14393 was characterized. The enzyme showed no substrate specificity toward D-galactose, which was similar to B. subtilis L-AI but distinguished from other reported L-AIs. The araA gene encoding the P. haloplanktis L-AI was cloned and overexpressed in E...
November 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/27546917/two-step-enzymatic-synthesis-of-6-deoxy-l-psicose
#20
Liuqing Wen, Kenneth Huang, Yuan Zheng, Junqiang Fang, Shukkoor Muhammed Kondengaden, Peng George Wang
Rare sugars offer a plethora of applications in the pharmaceutical, medicinal, and industries, as well as in synthetic chemistry. However, studies of rare sugars have been hampered by their relative scarcity. In this work, we describe a two-step strategy to efficiently and conveniently prepare 6-deoxy-L-psicose from L-rhamnose. In the first reaction step, the isomerization of L-rhamnose (6-deoxy-L-mannose) to L-rhamnulose (6-deoxy-L-fructose) catalyzed by L-rhamnose isomerase (RhaI), and the epimerization of L-rhamnulose to 6-deoxy-L-psicose catalyzed by D-tagatose 3-epimerase (DTE) were coupled with selective phosphorylation reaction by fructose kinase from human (HK), which selectively phosphorylate 6-deoxy-L-psicose at C-1 position...
August 24, 2016: Tetrahedron Letters
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