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https://www.readbyqxmd.com/read/28330264/construction-of-recombinant-sestc-saccharomyces-cerevisiae-for-consolidated-bioprocessing-cellulase-characterization-and-ethanol-production-by-in-situ-fermentation
#1
Peizhou Yang, Haifeng Zhang, Shaotong Jiang
Bioethanol is an important oil substitute produced by the sugar fermentation process. To improve the efficiency of cellulase expression of Saccharomyces cerevisiae, a eukaryotic expression vector harboring a single-enzyme-system-three-cellulase gene (sestc) was integrated into the S. cerevisiae genome by the protoplast method. Using PCR screening, RT-PCR, and "transparent circle" detection, several recombinant S. cerevisiae strains, capable of efficiently expressing the heterogeneous cellulase, were selected...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324771/synthesis-and-conformational-analysis-of-a-simplified-inositol-model-of-the-streptococcus-pneumoniae-19f-capsular-polysaccharide-repeating-unit
#2
Giorgio Catelani, Felicia D'Andrea, Lorenzo Guazzelli, Alessio Griselli, Nicola Testi, Maria Assunta Chiacchio, Laura Legnani, Lucio Toma
Carbohydrate mimics have been studied for a long time as useful sugar substitutes, both in the investigation of biological events and in the treatment of sugar-related diseases. Here we report further evaluation of the capabilities of inositols as carbohydrate substitutes. The conformational features of an inositol-model of a simplified repeating unit corresponding to the capsular polysaccharide of Streptococcus pneumoniae 19F has been evaluated by computational analysis, and compared to the native repeating unit...
March 14, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28320597/racial-or-ethnic-and-socioeconomic-inequalities-in-adherence-to-national-dietary-guidance-in-a-large-cohort-of-us-pregnant-women
#3
Lisa M Bodnar, Hyagriv N Simhan, Corette B Parker, Heather Meier, Brian M Mercer, William A Grobman, David M Haas, Deborah A Wing, Matthew K Hoffman, Samuel Parry, Robert M Silver, George R Saade, Ronald Wapner, Jay D Iams, Pathik D Wadhwa, Michal Elovitz, Alan M Peaceman, Sean Esplin, Shannon Barnes, Uma M Reddy
BACKGROUND: The significance of periconceptional nutrition for optimizing offspring and maternal health and reducing social inequalities warrants greater understanding of diet quality among US women. OBJECTIVE: Our objective was to evaluate racial or ethnic and education inequalities in periconceptional diet quality and sources of energy and micronutrients. DESIGN: Cross-sectional analysis of data from the Nulliparous Pregnancy Outcomes Study: Monitoring Mothers-to-Be cohort...
March 14, 2017: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/28318229/tracing-effects-of-fluorine-substitutions-on-g-quadruplex-conformational-changes
#4
Jonathan Dickerhoff, Linn Haase, Walter Langel, Klaus Weisz
A human telomere sequence that folds into an intramolecular (3+1)-hybrid G-quadruplex was modified by the incorporation of 2'-fluoro-2'-deoxyriboguanosines ((F)G) into syn positions of its outer tetrad. A circular dichroism and NMR spectral analysis reveals a nearly quantitative switch of the G-tetrad polarity with concerted syn↔anti transitions of all four G residues. These observations follow findings on a (F)G-substituted (3+1)-hybrid quadruplex with a different fold, suggesting a more general propensity of hybrid-type quadruplexes to undergo a tetrad polarity reversal...
March 20, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28317764/cucurbitane-glycosides-from-the-fruit-of-siraitia-grosvenori-and-their-effects-on-glucose-uptake-in-human-hepg2-cells-in-vitro
#5
Fu Li, Fumei Yang, Xin Liu, Lun Wang, Bin Chen, Lianhong Li, Mingkui Wang
The mogrosides in the fruit of Siraitia grosvenori can serve as a sugar substitute for diabetics due to their sweetness, low calorie and positive effects on blood glucose level control. The present study was to purify the mogrosides from the fruit of S. grosvenori and evaluate their enhancement of glucose uptake rate in HepG2 cells in vitro. As a result, eighteen mogrosides were isolated, including six new ones and a known but new naturally occurring compound. The chemical structures of the new compounds were identified by 1D, 2D-NMR and HR-ESI-MS techniques, together with chemical methods...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28302398/synthesis-of-isomeric-analogues-of-s-ribosylhomocysteine-analogues-with-homocysteine-unit-attached-to-c2-of-ribose
#6
Christiane Chbib
LuxS (S-ribosylhomocysteinase; EC 4.4.1.21) is an enzyme that catalyzes the cleavage of the thioether linkage in the catalytic pathway of S-ribosylhomocysteine (SRH) which produces homocysteine and 4,5-dihydroxy-2,3-pentanedione (DPD). DPD is the precursor of the signaling molecules known as autoinducer 2 (AI-2) responsible for the bacterial quorum sensing (QS) identified as cell to cell communication. Inhibitors of LuxS should be able to interfere with its catalytic pathway thus preventing the formation of the autoinducer molecules...
March 6, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28286650/review-sugar-beets-as-a-substitute-for-grain-for-lactating-dairy-cattle
#7
REVIEW
Essi Evans, Ulrike Messerschmidt
Dairy cows are customarily given grains and highly digestible byproduct ingredients as additions to forage to support milk production. In many parts of the world growing seasons are short, and the grain crops that can be grown may not provide adequate yields. Sugar beets, on the other hand are relatively hardy, and dry matter yields surpass the yields of most grain crops. There are however, perceptions that beets may not be suitable as a feed ingredient due to the fact that the storage form of carbohydrate is sugar rather than starch...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28255654/replacement-of-glycaemic-carbohydrates-by-inulin-type-fructans-from-chicory-oligofructose-inulin-reduces-the-postprandial-blood-glucose-and-insulin-response-to-foods-report-of-two-double-blind-randomized-controlled-trials
#8
Helen Lightowler, Sangeetha Thondre, Anja Holz, Stephan Theis
PURPOSE: Inulin-type fructans are recognized as prebiotic dietary fibres and classified as non-digestible carbohydrates that do not contribute to glycaemia. The aim of the present studies was to investigate the glycaemic response (GR) and insulinaemic response (IR) to foods in which sucrose was partially replaced by inulin or oligofructose from chicory. METHODS: In a double-blind, randomized, controlled cross-over design, 40-42 healthy adults consumed a yogurt drink containing oligofructose or fruit jelly containing inulin and the respective full-sugar variants...
March 3, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28254574/structural-insight-of-an-antioxidative-arabinogalactan-protein-of-aegle-marmelos-fruit-gum-and-it-s-interaction-with-%C3%AE-lactoglobulin
#9
Kaushik Bera, Sayani Ray, Washim Raja, Bimalendu Ray
The complication of arabinogalactan protein (AGP) structure, a significant ingredient of gum polysaccharides, not merely hinders the allocation of its role, but restricts its utilization as well. Here, we describe structural details of an AGP purified from Aegle marmelos fruit gum. This AGP (310×10(3)g/mol), which is water-soluble, contains β-1,3-linked galactopyranosyl main chain substituted at O-6 position with side chains containing galactose and arabinose residues. Also data on sugar composition, ring size, glycosidic linkage pattern, anomeric configuration and sequence of monosaccharide units of a number of oligosaccharides produced from this AGP by chemical and enzymatic methods were acquired...
February 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28243931/effect-of-sugar-industry-spentwash-diluted-on-the-characteristics-of-soil-and-sugarcane-saccharum-officinarum-l-growth-in-the-subtropical-environment-of-sindh-pakistan
#10
Ghulam Muhiyuddin Kaloi, Mehrunisa Memon, Kazi Suleman Memon, Sagheer Ahmad, Saghir Ahmed Sheikh, Ghulam Murtaza Jamro
Spentwash is a rich source of organic matter and essential plant nutrients in addition to excess salts. Sugar mills in Pakistan discharge about 3.48 million tons of spentwash annually, with no proper procedures for its disposal or utilization. To test the effect of diluted spentwash on soil and the soil's ability to support plant growth, sugarcane (Saccharum officinarum L.) variety CPF-237 was planted. The experiment was conducted in a randomized complete block design involving factorial combination of four concentrations of each spentwash (0, 10, 20, and 30%) and mineral fertilizers (0, 1/3, 2/3, and the full recommended rate of NP)...
March 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28218965/physicochemical-characterization-of-a-heat-treated-calcium-alginate-dry-film-prepared-with-chicken-stock
#11
Germán D Báez, Gisela N Piccirilli, Griselda A Ballerini, Agustín Frattini, Pablo A Busti, Roxana A Verdini, Néstor J Delorenzi
Solid sodium alginate was dissolved into chicken stock in order to give a final alginate concentration of 0.9 percent (w/v). Calcium ions present in chicken stock were enough to induce ionic gelation. After drying, Fourier transform infrared spectroscopy, thickness and mechanical properties of films obtained were determined. Calcium alginate-chicken stock films were heated at 130 °C for different times between 0 and 15 min. Mechanical and optical studies, differential scanning calorimetry, visual aspect and scanning electron microscopy were carried out to describe physicochemical properties of heat treated films...
February 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28216580/impacts-of-dietary-phytochemicals-in-the-presence-and-absence-of-pesticides-on-longevity-of-honey-bees-apis-mellifera
#12
Ling-Hsiu Liao, Wen-Yen Wu, May R Berenbaum
Because certain flavonols and phenolic acids are found in pollen and nectar of most angiosperms, they are routinely ingested by Apis mellifera, the western honey bee. The flavonol quercetin and the phenolic acid p-coumaric acid are known to upregulate detoxification enzymes in adult bees; their presence or absence in the diet may thus affect the toxicity of ingested pesticides. We conducted a series of longevity assays with one-day-old adult workers to test if dietary phytochemicals enhance longevity and pesticide tolerance...
February 14, 2017: Insects
https://www.readbyqxmd.com/read/28214853/chronic-consumption-of-artificial-sweetener-in-packets-or-tablets-and-type-2-diabetes-risk-evidence-from-the-e3n-european-prospective-investigation-into-cancer-and-nutrition-study
#13
Guy Fagherazzi, Gaëlle Gusto, Aurélie Affret, Francesca Romana Mancini, Courtney Dow, Beverley Balkau, Françoise Clavel-Chapelon, Fabrice Bonnet, Marie-Christine Boutron-Ruault
BACKGROUND: The influence of artificial sweeteners on metabolic diseases is controversial. Artificially sweetened beverages have been associated with an increased risk of type 2 diabetes (T2D) but biases and reverse causation have been suspected to have influenced the observed association. In addition, it has been suggested that investigation into the relationship between the frequency and duration of the consumption of packet or tablet artificial sweeteners and T2D risk is necessary...
February 18, 2017: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/28193207/a-novel-point-mutation-in-rpob-improves-osmotolerance-and-succinic-acid-production-in-escherichia-coli
#14
Mengyong Xiao, Xinna Zhu, Hongtao Xu, Jinlei Tang, Ru Liu, Changhao Bi, Feiyu Fan, Xueli Zhang
BACKGROUND: Escherichia coli suffer from osmotic stress during succinic acid (SA) production, which reduces the performance of this microbial factory. RESULTS: Here, we report that a point mutation leading to a single amino acid change (D654Y) within the β-subunit of DNA-dependent RNA polymerase (RpoB) significantly improved the osmotolerance of E. coli. Importation of the D654Y mutation of RpoB into the parental strain, Suc-T110, increased cell growth and SA production by more than 40% compared to that of the control under high glucose osmolality...
February 13, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28192727/ecotoxicological-assessments-show-sucralose-and-fluoxetine-affect-the-aquatic-plant-lemna-minor
#15
Cherisse Amy-Sagers, Keith Reinhardt, Danelle M Larson
Pharmaceuticals and personal care products (PPCP) are prevalent in aquatic systems, yet the fate and impacts on aquatic plants needs quantification for many compounds. We measured and detected sucralose (an artificial sweetener), fluoxetine (an antidepressant), and other PPCP in the Portneuf River in Idaho, USA, where Lemna minor (an aquatic plant in the environment and used in ecotoxicology studies) naturally occurs. Sucralose was hypothesized to negatively affect photosynthesis and growth of L. minor because sucralose is a chlorinated molecule that may be toxic or unusable for plant metabolism...
April 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28188667/chronic-condition-as-a-mediator-between-metabolic-syndrome-and-cognition-among-community-dwelling-older-adults-the-moderating-role-of-sex
#16
Hui Foh Foong, Tengku Aizan Hamid, Rahimah Ibrahim, Sharifah Azizah Haron, Suzana Shahar
AIM: Metabolic syndrome and chronic conditions are significant predictors of cognition; however, few studies have examined how they work together in predicting cognition in old age. Therefore, the present study examines whether a chronic condition mediates the association between metabolic syndrome and cognition. In addition, it discusses the moderating role of sex in the relationships between metabolic syndrome, chronic conditions and cognition. METHODS: Secondary analysis was carried out of data from the Malaysian national survey that involved 2322 community residents aged 60 years or older in Peninsular Malaysia...
February 11, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/28183287/price-elasticity-of-the-demand-for-soft-drinks-other-sugar-sweetened-beverages-and-energy-dense-food-in-chile
#17
Carlos M Guerrero-López, Mishel Unar-Munguía, M Arantxa Colchero
BACKGROUND: Chile is the second world's largest per capita consumer of caloric beverages. Caloric beverages are associated with overweight, obesity and other chronic diseases. The objective of this study is to estimate the price elasticity of demand for soft drinks, other sugar-sweetened beverages and high-energy dense foods in urban areas in Chile in order to evaluate the potential response of households' consumption to changes in prices. METHODS: We used microdata from the VII Family Budget Survey 2012-2013, which collects information on expenditures made by Chilean urban households on items such as beverages and foods...
February 10, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28160110/brettanomyces-acidodurans-sp-nov-a-new-acetic-acid-producing-yeast-species-from-olive-oil
#18
Gábor Péter, Dénes Dlauchy, Andrea Tóbiás, László Fülöp, Martina Podgoršek, Neža Čadež
Two yeast strains representing a hitherto undescribed yeast species were isolated from olive oil and spoiled olive oil originating from Spain and Israel, respectively. Both strains are strong acetic acid producers, equipped with considerable tolerance to acetic acid. The cultures are not short-lived. Cellobiose is fermented as well as several other sugars. The sequences of their large subunit (LSU) rRNA gene D1/D2 domain are very divergent from the sequences available in the GenBank. They differ from the closest hit, Brettanomyces naardenensis by about 27%, mainly substitutions...
February 3, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28155810/fungal-enzymes-and-yeasts-for-conversion-of-plant-biomass-to-bioenergy-and-high-value-products
#19
Lene Lange
Fungi and fungal enzymes play important roles in the new bioeconomy. Enzymes from filamentous fungi can unlock the potential of recalcitrant lignocellulose structures of plant cell walls as a new resource, and fungi such as yeast can produce bioethanol from the sugars released after enzyme treatment. Such processes reflect inherent characteristics of the fungal way of life, namely, that fungi as heterotrophic organisms must break down complex carbon structures of organic materials to satisfy their need for carbon and nitrogen for growth and reproduction...
January 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28126702/stevia-based-sweeteners-as-a-promising-alternative-to-table-sugar-the-effect-on-listeria-monocytogenes-and-salmonella-typhimurium-growth-dynamics
#20
María M Lobete, Maria Baka, Estefanía Noriega, Etienne Jooken, Annick Monballiu, Sam de Beurme, Boudewijn Meesschaert, Jan F Van Impe
Sugar is commonly substituted with stevia-based products in food industry and in our daily-life. This substitution results in a change in food product characteristic formula and properties that may affect the growth dynamics of food pathogenic and spoilage bacteria. This work studies the effect of table sugar (TS), laboratory sucrose (LS), commercial stevia (St) and steviol glycosides (SG) on the growth dynamics of Salmonella Typhimurium and Listeria monocytogenes. Experiments were carried out in general and minimal culture media at 3 equivalent concentration levels in terms of sweetness intensity (TS and LS at 3, 9 and 15% (w/v); St at 0...
March 20, 2017: International Journal of Food Microbiology
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