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https://www.readbyqxmd.com/read/28512318/structure-based-prediction-and-identification-of-4-epimerization-activity-of-phosphate-sugars-in-class-ii-aldolases
#1
Seon-Hwa Lee, Seung-Hye Hong, Jung-Ung An, Kyoung-Rok Kim, Dong-Eun Kim, Lin-Woo Kang, Deok-Kun Oh
Sugar 4-epimerization reactions are important for the production of rare sugars and their derivatives, which have various potential industrial applications. For example, the production of tagatose, a functional sweetener, from fructose by sugar 4-epimerization is currently constrained because a fructose 4-epimerase does not exist in nature. We found that class II D-fructose-1,6-bisphosphate aldolase (FbaA) catalyzed the 4-epimerization of D-fructose-6-phosphate (F6P) to D-tagatose-6-phosphate (T6P) based on the prediction via structural comparisons with epimerase and molecular docking and the identification of the condensed products of C3 sugars...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28482458/potential-of-vegetated-ditches-to-manage-organic-pollutants-derived-from-agricultural-runoff-and-domestic-sewage-a-case-study-in-sinaloa-mexico
#2
Monika Moeder, Otoniel Carranza-Diaz, Gabriela López-Angulo, Rito Vega-Aviña, Francisco Armando Chávez-Durán, Seifeddine Jomaa, Ursula Winkler, Steffi Schrader, Thorsten Reemtsma, Francisco Delgado-Vargas
This case study presents the fate of selected organic, priority and emerging pollutants along a 3.6km sector of a vegetated, agricultural ditch situated in Sinaloa (Mexico). The ditch receives runoff of agriculture and domestic wastewater from an adjacent community. During 2013, the occurrence of 38 organic pollutants (pesticides, polycyclic aromatic hydrocarbons (PAHs), artificial sweeteners and pharmaceutical residues) was monitored monthly at five selected points in the ditch water. Additionally, sediment and Typha domingensis (cattail) plants were collected in March, June, and September 2013 and investigated concerning their ability to absorb and accumulate pollutants...
May 2, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28481230/the-effect-of-isomaltulose-together-with-green-tea-on-glycemic-response-and-antioxidant-capacity-a-single-blind-crossover-study-in-healthy-subjects
#3
Passakorn Suraphad, Phim On Suklaew, Sathaporn Ngamukote, Sirichai Adisakwattana, Kittana Mäkynen
Isomaltulose, a naturally-occurring isomer of sucrose, is commonly used as an alternative sweetener in foods and beverages. The goal of this study was to determine the effect of isomaltulose together with green tea on postprandial plasma glucose and insulin concentration, as well as antioxidant capacity in healthy subjects. In a randomized, single-blind, crossover study, 15 healthy subjects (eight women and seven men; ages 23.5 ± 0.7 years; with body mass index of 22.6 ± 0.4 kg/m²) consumed five beverages: (1) 50 g sucrose in 400 mL water; (2) 50 g isomaltulose in 400 mL of water; (3) 400 mL of green tea; (4) 50 g sucrose in 400 mL of green tea; and (5) 50 g isomaltulose in 400 mL of green tea...
May 6, 2017: Nutrients
https://www.readbyqxmd.com/read/28434726/short-communication-sensory-analysis-of-a-kefir-product-designed-for-active-cancer-survivors
#4
K O'Brien, C Boeneke, W Prinyawiwatkul, J Lisano, D Shackelford, K Reeves, M Christensen, R Hayward, K Carabante Ordonez, L K Stewart
Kefir is a fermented milk product that is a good source of protein and health-promoting bacteria. It has the potential to improve recovery from exercise, and the health and well-being of cancer survivors. The purpose of this study was to explore cancer survivor attitudes about and acceptance of a kefir recovery beverage made from cultured milk, whole fruit, natural sweeteners, and other natural ingredients. Kefir was made by inoculating and fermenting milk with kefir grains. The kefir was then mixed with a fruit base and given to cancer survivors (n = 52) following a bout of exercise...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28399881/the-influence-of-parental-practices-on-child-promotive-and-preventive-food-consumption-behaviors-a-systematic-review-and-meta-analysis
#5
REVIEW
Andrew Z H Yee, May O Lwin, Shirley S Ho
BACKGROUND: The family is an important social context where children learn and adopt eating behaviors. Specifically, parents play the role of health promoters, role models, and educators in the lives of children, influencing their food cognitions and choices. This study attempts to systematically review empirical studies examining the influence of parents on child food consumption behavior in two contexts: one promotive in nature (e.g., healthy food), and the other preventive in nature (e...
April 11, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28399012/sweeteners-and-sweetness-enhancers
#6
Christine Belloir, Fabrice Neiers, Loïc Briand
PURPOSE OF REVIEW: The current review summarizes and discusses current knowledge on sweeteners and sweetness enhancers. RECENT FINDINGS: The perception of sweet taste is mediated by the type 1 taste receptor 2 (T1R2)/type 1 taste receptor 3 (T1R3) receptor, which is expressed in the oral cavity, where it provides input on the caloric and macronutrient contents of ingested food. This receptor recognizes all the compounds (natural or artificial) perceived as sweet by people...
April 8, 2017: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/28394355/a-natural-food-sweetener-with-anti-pancreatic-cancer-properties
#7
C Liu, L-H Dai, D-Q Dou, L-Q Ma, Y-X Sun
No abstract text is available yet for this article.
April 10, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28378852/effects-of-non-nutritive-artificial-vs-natural-sweeteners-on-24-h-glucose-profiles
#8
S L Tey, N B Salleh, C J Henry, C G Forde
Replacing nutritive sweetener with non-nutritive sweeteners (NNS) has the potential to improve glycaemic control. The objective of this study was to investigate the effects of consuming artificial NNS (that is, aspartame), natural NNS (that is, monk fruit and stevia), and sucrose-sweetened beverages on 24-h glucose profiles. Ten healthy males took part in this randomised, crossover study with the following four treatments: aspartame-, monk fruit-, stevia-, and sucrose- (65 g) sweetened beverages. Participants were asked to consume the test beverage as a preload mid-morning...
April 5, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28370108/a-preliminary-study-on-the-application-of-natural-sweet-proteins-in-agar-based-gels
#9
Nicoletta A Miele, Rossella Di Monaco, Francesca Dell'Amura, Michele F Rega, Delia Picone, Silvana Cavella
Natural sweet proteins may be used as sugar replacer in simple liquid food systems but their applicability in more complex matrices has not been investigated yet. Gelling agent nature and texture characteristics as well as type and distribution of a stimulus in a gel could affect taste perception through inhibition or enhancement of tastants migration to the receptors. The mechanical, nonoral texture and time-intensity sweetness characteristics of sweet proteins MNEI and super sweet Y65R mutant, aspartame and saccharin added at a concentration iso-sweet to 40 g/L of sucrose in three agar gel concentrations (1%, 1...
April 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/28361903/steviol-glycosides-enhance-pancreatic-beta-cell-function-and-taste-sensation-by-potentiation-of-trpm5-channel-activity
#10
Koenraad Philippaert, Andy Pironet, Margot Mesuere, William Sones, Laura Vermeiren, Sara Kerselaers, Sílvia Pinto, Andrei Segal, Nancy Antoine, Conny Gysemans, Jos Laureys, Katleen Lemaire, Patrick Gilon, Eva Cuypers, Jan Tytgat, Chantal Mathieu, Frans Schuit, Patrik Rorsman, Karel Talavera, Thomas Voets, Rudi Vennekens
Steviol glycosides (SGs), such as stevioside and rebaudioside A, are natural, non-caloric sweet-tasting organic molecules, present in extracts of the scrub plant Stevia rebaudiana, which are widely used as sweeteners in consumer foods and beverages. TRPM5 is a Ca(2+)-activated cation channel expressed in type II taste receptor cells and pancreatic β-cells. Here we show that stevioside, rebaudioside A and their aglycon steviol potentiate the activity of TRPM5. We find that SGs potentiate perception of bitter, sweet and umami taste, and enhance glucose-induced insulin secretion in a Trpm5-dependent manner...
March 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/28352301/functional-overexpression-of-genes-involved-in-erythritol-synthesis-in-the-yeast-yarrowia-lipolytica
#11
Aleksandra M Mirończuk, Anna Biegalska, Adam Dobrowolski
BACKGROUND: Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28348965/in-vitro-and-in-vivo-assessment-of-inhibitory-effect-of-stevioside-on-pro-inflammatory-cytokines
#12
Jatuporn Noosud, Narissara Lailerd, Autchara Kayan, Chaiwat Boonkaewwan
OBJECTIVE: Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity. The aim of the present study was to examine in vitro and in vivo effects of stevioside on rats plasma levels of tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs). MATERIALS AND METHODS: Male wistar rats weighing between 170-220 g were given stevioside (0, 500 and 1000 mg/kg BW/day) for 6 weeks...
March 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28339377/stability-of-clindamycin-hydrochloride-in-pcca-base-suspendit
#13
Yashoda V Pramar, Richard A Graves, Grace A Ledet, Kelly V Phan, Levon A Bostanian, Tarun K Mandal
Clindamycin is an effective antibiotic in the treatment of infections caused by certain gram-positive and gram-negative anaerobic microorganisms. While manufactured forms of the drug for pediatric use are available, there are instances when a compounded liquid dosage form is essential to meet unique patient needs. The purpose of this study was to determine the chemical stability of clindamycin hydrochloride in the PCCA base SuspendIt, a sugar-free, paraben- free, dye-free, and gluten-free thixotropic vehicle containing a natural sweetener obtained from the monk fruit...
September 2016: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/28303700/evaluation-of-dairy-products-available-on-the-polish-market-in-the-context-of-nutrient-profiles-clear-arguments-for-reformulation-of-foodstuffs
#14
Regina Wierzejska, Magdalena Siuba-Strzelińska, Mirosław Jarosz
Background: Dairy products, which are one of the main groups of products in the diet, are expected to have high nutritional value. The development of food technology often involves changing the nutritional parameters of foodstuffs. Objective: The aim of this study was to determine the nutritional value of dairy products, in the context of nutrient profiles. Material and Methods: The tested products included yoghurts, yoghurt drinks, kefir, cream cheeses and milk desserts available on the Polish market, basing on the information from unit product packaging...
2017: Roczniki Państwowego Zakładu Higieny
https://www.readbyqxmd.com/read/28284644/suitability-of-pharmaceuticals-and-personal-care-products-ppcps-and-artificial-sweeteners-ass-as-wastewater-indicators-in-the-pearl-river-delta-south-china
#15
Yuan-Yuan Yang, Wang-Rong Liu, You-Sheng Liu, Jian-Liang Zhao, Qian-Qian Zhang, Min Zhang, Jin-Na Zhang, Yu-Xia Jiang, Li-Juan Zhang, Guang-Guo Ying
Wastewater indicator is a useful tool for evaluating the wastewater impact on natural water, but there is little information about the suitability of wastewater indicators for different regions. This study aimed to select suitable wastewater indicators in the Pearl River Delta region, south China by screening a range of wastewater related organic compounds. The screening campaign was carried out by investigating the occurrence and removal efficiencies of 93 pharmaceuticals and personal care products (PPCPs) and 5 artificial sweeteners (ASs) in nine wastewater treatment plants (WWTPs) located in the region, and the occurrence of these target compounds in the contaminated and clean surface water of the Pearl River...
March 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28272545/glycyrrhiza-uralensis-water-extract-enhances-dendritic-cell-maturation-and-antitumor-efficacy-of-hpv-dendritic-cell-based-vaccine
#16
Adila Aipire, Jinyu Li, Pengfei Yuan, Jiang He, Yelang Hu, Lu Liu, Xiaoli Feng, Yijie Li, Fuchun Zhang, Jianhua Yang, Jinyao Li
Licorice has been used as herbal medicine and natural sweetener. Here, we prepared Glycyrrhiza uralensis water extract (GUWE) and investigated the effect of GUWE on the maturation and function of dendritic cells (DCs) and its adjuvant effect on DC-based vaccine. We observed that GUWE dose-dependently promoted DC maturation and cytokine secretion through TLR4 signaling pathway. The capacity of DC to stimulate allogenic splenocyte proliferation was also enhanced by GUWE treatment. Compared with control group, GUWE treated DCs pulsed with human papillomavirus (HPV)-16 E6/E7 peptides significantly inhibited the tumor growth in both early and late therapeutic groups...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28251728/tandem-mass-spectrometry-a-convenient-approach-in-the-dosage-of-steviol-glycosides-in-stevia-sweetened-commercial-food-beverages
#17
L Di Donna, F Mazzotti, I Santoro, G Sindona
The use of sweeteners extracted from leaves of the plant species Stevia rebaudiana is increasing worldwide. They are recognized as generally recognized as safe by the US-FDA and approved by EU-European Food Safety Authority, with some recommendation on the daily dosage that should not interfere with glucose metabolism. The results presented here introduce an easy analytical approach for the identification and assay of Stevia sweeteners in commercially available soft drink, based on liquid chromatography coupled to tandem mass spectrometry, using a natural statin-like molecule, Brutieridin, as internal standard...
May 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28251536/ecotoxicological-survey-of-mnei-and-y65r-mnei-proteins-as-new-potential-high-intensity-sweeteners
#18
Michele Fortunato Rega, Antonietta Siciliano, Renato Gesuele, Giusy Lofrano, Andrea Carpentieri, Delia Picone, Marco Guida
Low-calorie sweeteners are widespread. They are routinely introduced into commonly consumed food such as diet sodas, cereals, and sugar-free desserts. Recent data revealed the presence in considerable quantities of some of these artificial sweeteners in water samples qualifying them as a class of potential new emerging contaminants. This study aimed at evaluating the ecotoxicity profile of MNEI and Y65R-MNEI, two engineered products derived from the natural protein monellin, employing representative test organism such as Daphnia magna, Ceriodaphnia dubia, and Raphidocelis subcapitata...
March 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28218969/stability-of-the-stevia-derived-sweetener-rebaudioside-a-in-solution-as-affected-by-ultraviolet-light-exposure
#19
Jiewen Zhang, Leonard N Bell
Rebaudioside A is a natural noncaloric high-potency sweetener extracted from the leaves of Stevia rebaudiana. With rebaudioside A use increasing in foods, understanding the factors affecting its stability is necessary. This project evaluated the degradation rate constants of rebaudioside A in water, 0.1 M phosphate buffer, and 0.1 M citrate buffer at pH 3 and 7 as a function of ultraviolet (UV) light intensity (365 nm, 0 μW/cm(2) for dark conditions, 27 μW/cm(2) for low intensity, and 190 μW/cm(2) for high intensity) at 32...
April 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28215607/impact-of-blue-red-and-far-red-light-treatments-on-gene-expression-and-steviol-glycoside-accumulation-in-stevia-rebaudiana
#20
Yuki Yoneda, Hiroshi Nakashima, Juro Miyasaka, Katsuaki Ohdoi, Hiroshi Shimizu
Stevia rebaudiana (Bertoni) Bertoni is a plant that biosynthesizes a group of natural sweeteners that are up to approximately 400 times sweeter than sucrose. The sweetening components of S. rebaudiana are steviol glycosides (SGs) that partially share their biosynthesis pathway with gibberellins (GAs). However, the molecular mechanisms through which SGs levels can be improved have not been studied. Therefore, transcription levels of several SG biosynthesis-related genes were analyzed under several light treatments involved in GA biosynthesis...
May 2017: Phytochemistry
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