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https://www.readbyqxmd.com/read/28719181/differential-ligand-binding-specificities-of-the-pulmonary-collectins-are-determined-by-the-conformational-freedom-of-a-surface-loop
#1
Michael J Rynkiewicz, Huixing Wu, Tanya R Cafarella, Nikolaos M Nikolaidis, James Frederick Head, Barbara A Seaton, Francis X McCormack
Lung surfactant proteins (SP) play critical roles in surfactant function and innate immunity. SP-A and SP-D, members of the collectin family of C-type lectins, exhibit distinct ligand specificities, effects on surfactant structure, and host defense functions despite extensive structural homology. SP-A binds to dipalmitoylphosphatidylcholine (DPPC), the major surfactant lipid component, but not phosphatidylinositol (PI), whereas SP-D shows the opposite preference. Additionally, SP-A and SP-D recognize widely divergent pathogen-associated molecular patterns...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28717253/prostacyclin-reverses-platelet-stress-fibre-formation-causing-platelet-aggregate-instability
#2
M Z Yusuf, Z Raslan, L Atkinson, A Aburima, S G Thomas, K M Naseem, S D J Calaminus
Prostacyclin (PGI2) modulates platelet activation to regulate haemostasis. Evidence has emerged to suggest that thrombi are dynamic structures with distinct areas of differing platelet activation. It was hypothesised that PGI2 could reverse platelet spreading by actin cytoskeletal modulation, leading to reduced capability of platelet aggregates to withstand a high shear environment. Our data demonstrates that post-flow of PGI2 over activated and spread platelets on fibrinogen, identified a significant reduction in platelet surface area under high shear...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28714909/the-interplay-between-the-host-receptor-and-influenza-virus-hemagglutinin-and-neuraminidase
#3
REVIEW
Lauren Byrd-Leotis, Richard D Cummings, David A Steinhauer
The hemagglutinin (HA) and neuraminidase (NA) glycoproteins of influenza A virus are responsible for the surface interactions of the virion with the host. Entry of the virus is mediated by functions of the HA: binding to cellular receptors and facilitating fusion of the virion membrane with the endosomal membrane. The HA structure contains receptor binding sites in the globular membrane distal head domains of the trimer, and the fusion machinery resides in the stem region. These sites have specific characteristics associated with subtype and host, and the differences often define species barriers...
July 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28714554/type-ix-secretion-the-generation-of-bacterial-cell-surface-coatings-involved-in-virulence-gliding-motility-and-the-degradation-of-complex-biopolymers
#4
Paul D Veith, Michelle D Glew, Dhana G Gorasia, Eric C Reynolds
The Type IX Secretion System (T9SS) is present in over 1000 sequenced species/strains of the Fibrobacteres-Chlorobi-Bacteroidetes superphylum. Proteins secreted by the T9SS have an N-terminal signal peptide for translocation across the inner membrane via the SEC translocon and a C-terminal signal for secretion across the outer membrane via the T9SS. Nineteen protein components of the T9SS have been identified including three, SigP, PorX and PorY that are involved in regulation. The inner membrane proteins PorL and PorM and the outer membrane proteins PorK and PorN interact and a complex comprising PorK and PorN forms a large ring structure of 50 nm in diameter...
July 17, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28713858/life-stage-specific-cargo-receptors-facilitate-glycosylphosphatidylinositol-anchored-surface-coat-protein-transport-in-trypanosoma-brucei
#5
Emilia K Kruzel, George P Zimmett, James D Bangs
The critical virulence factor of bloodstream-form Trypanosoma brucei is the glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG). Endoplasmic reticulum (ER) exit of VSG is GPI dependent and relies on a discrete subset of COPII machinery (TbSec23.2/TbSec24.1). In other systems, p24 transmembrane adaptor proteins selectively recruit GPI-anchored cargo into nascent COPII vesicles. Trypanosomes have eight putative p24s (TbERP1 to TbERP8) that are constitutively expressed at the mRNA level...
July 2017: MSphere
https://www.readbyqxmd.com/read/28712304/regulation-of-cellular-redox-signaling-by-matricellular-proteins-in-vascular-biology-immunology-and-cancer
#6
David D Roberts, Sukhbir Kaur, Jeffrey S Isenberg
SIGNIFICANCE: In contrast to structural elements of the extracellular matrix, matricellular proteins appear transiently during development and injury responses, but their sustained expression can contribute to chronic disease. Through interactions with other matrix components and specific cell surface receptors matricellular proteins regulate multiple signaling pathways, including those mediated by reactive oxygen and nitrogen species and H2S. Dysregulation of matricellular proteins contributes to the pathogenesis of vascular diseases and cancer...
July 16, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28710251/role-for-zap-70-signaling-in-the-differential-effector-functions-of-rituximab-and-obinutuzumab-ga101-in-chronic-lymphocytic-leukemia-b-cells
#7
Sladjana Skopelja-Gardner, Jonathan D Jones, B JoNell Hamilton, Alexey V Danilov, William F C Rigby
Rituximab (RTX) has been the hallmark anti-CD20 mAb for the treatment of B cell neoplasms, including B cell chronic lymphocytic leukemia (B-CLL). Recently, a novel humanized anti-CD20 mAb obinutuzumab (GA101) has been implemented as first-line CLL therapy. Treatment of CLL patients with RTX is associated with CD20 loss via an FcγR-mediated process, trogocytosis. RTX-induced trogocytosis has been characterized as both the means of resistance to therapy, via loss of cell surface target proteins (antigenic modulation), as well as a process that alters B cell phenotype and function...
July 14, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28709377/combining-graphical-and-analytical-methods-with-molecular-simulations-to-analyze-time-resolved-fret-measurements-of-labeled-macromolecules-accurately
#8
Thomas-Otavio Peulen, Oleg Opanasyuk, Claus A M Seidel
Förster resonance energy transfer (FRET) measurements from a donor, D, to an acceptor, A, fluorophore are frequently used in vitro and in live cells to reveal information on the structure and dynamics of DA-labeled macromolecules. Accurate descriptions of FRET-measurements by molecular models are complicated because the fluorophores are usually coupled to the macromolecule via flexible long linkers allowing for diffusional exchange between multiple states with different fluorescence properties caused by distinct environmental quenching, dye mobilities, and variable DA-distances...
July 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28708592/a-peptidomimetic-with-a-chiral-switch-is-an-inhibitor-of-epidermal-growth-factor-receptor-heterodimerization
#9
Shanthi P Kanthala, Yong-Yu Liu, Sitanshu Singh, Rushikesh Sable, Sandeep Pallerla, Seetharama D Jois
Among different types of EGFR dimers, EGFR-HER2 and HER2-HER3 are well known in different types of cancers. Targeting dimerization of EGFR will have a significant impact on cancer therapies. A symmetric peptidomimetic was designed to inhibit the protein-protein interaction of EGFR. The peptidomimetic (Cyclo(1,10)PpR (R) Anapa-FDDF-(R)-Anapa)R, compound 18) was shown to exhibit antiproliferative activity with an IC50 of 194 nM in HER2-expressing breast cancer cell lines and 18 nM in lung cancer cell lines. The peptidomimetic has a Pro-Pro sequence in the structure to stabilize the β-turn and a β-amino acid, amino napthyl propionic acid...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28707606/evidence-of-positive-selection-towards-zebuine-haplotypes-in-the-bola-region-of-brangus-cattle
#10
D E Goszczynski, C M Corbi-Botto, H M Durand, A Rogberg-Muñoz, S Munilla, P Peral-Garcia, R J C Cantet, G Giovambattista
The Brangus breed was developed to combine the superior characteristics of both of its founder breeds, Angus and Brahman. It combines the high adaptability to tropical and subtropical environments, disease resistance, and overall hardiness of Zebu cattle with the reproductive potential and carcass quality of Angus. It is known that the major histocompatibility complex (MHC, also known as bovine leucocyte antigen: BoLA), located on chromosome 23, encodes several genes involved in the adaptive immune response and may be responsible for adaptation to harsh environments...
July 14, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28704713/cation-chelation-and-ph-induced-controlled-switching-of-the-non-fouling-properties-of-bacterial-crystalline-films
#11
Jagoba Iturri, Alberto Moreno-Cencerrado, José L Toca-Herrera
We report the controlled loss of the anti-fouling activity of the S-layer protein SbpA from Lysinibacillus sphaericus (CCM2177). This protein forms crystal-like films with square lattice (p4) via self-assembly on almost any type of surfaces. Such engineered bioinspired nanometric membranes are known by their excellent preventive performance under biological conditions. However, their exposure to certain treatments can lead to gradual degradation of the S-protein layer. In this work, two distinctive approaches are studied for understanding either specific or non-specific degradation of the film, by treatment with a chelating agent (EDTA), which interacts with inner Ca(2+) ions, or Citrate buffer (with pH<pI), respectively...
July 3, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28702661/clinically-approved-pegylated-nanoparticles-are-covered-by-a-protein-corona-that-boosts-the-uptake-by-cancer-cells
#12
M Papi, D Caputo, V Palmieri, R Coppola, S Palchetti, F Bugli, C Martini, L Digiacomo, D Pozzi, G Caracciolo
Today, liposomes are an advanced technology of drug carriers with a dozen drugs in clinical practice and many more in clinical trials. A bottleneck associated with the clinical translation of liposomes has long been 'opsonization', i.e. the adsorption of plasma proteins at the liposome surface resulting in their rapid clearance from circulation. For decades, the most popular way to avoid opsonization has been grafting polyethylene glycol (PEG) onto the liposome surface. Recent studies have clarified that grafting PEG onto the liposome surface reduces, but does not completely prevent protein binding...
July 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/28702124/6-hydroxy-5-nitrobenzo-d-oxazol-2-3h-one-a-degradable-derivative-of-natural-6-hydroxybenzoxazolin-2-3h-one-produced-by-pantoea-ananatis
#13
Margot Schulz, Dieter Sicker, Oliver Schackow, Lothar Hennig, Diana Hofmann, Ulrich Disko, Marina Ventura, Kateryna Basyuk
Pantoea ananatis is a bacterium associated with other microorganisms on Abutilon theophrasti Medik. roots. It converts 6-hydroxybenzoxazolin-2(3H)-one (BOA-6-OH), a hydroxylated derivative of the allelochemical benzoxazolin-2(3H)-one, into 6-hydroxy-5-nitrobenzo[d]oxazol-2(3H)-one. The compound was identified by NMR and mass spectrometric methods. In vitro synthesis succeeded with Pantoea protein, with isolated proteins from the Abutilon root surface or with horseradish peroxidase in the presence of nitrite and H2O2...
2017: Communicative & Integrative Biology
https://www.readbyqxmd.com/read/28702111/innate-function-of-house-dust-mite-allergens-robust-enzymatic-degradation-of-extracellular-matrix-at-elevated-ph
#14
Kumiko Oida, Lukas Einhorn, Ina Herrmann, Lucia Panakova, Yvonne Resch, Susanne Vrtala, Gerlinde Hofstetter, Akane Tanaka, Hiroshi Matsuda, Erika Jensen-Jarolim
BACKGROUND: Exposure to the house dust mite Dermatophagoides pteronyssinus (D.p.) increases the risk for developing allergic diseases in humans and their best friends, the dogs. Here, we explored whether this allergenic mite via its enzymes may impact the cutaneous extracellular matrix (ECM), which critically determines epithelial barrier integrity both structurally and functionally. METHODS: Two extracts obtained from either dust-purified or cultured D.p. bodies were used in the present study...
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/28701393/hiv-1-env-and-vpu-specific-antibody-dependent-cellular-cytotoxicity-responses-associated-with-elite-control-of-hiv
#15
Vijaya Madhavi, Bruce D Wines, Janaki Amin, Sean Emery, Ester Lopez, Anthony Kelleher, Rob J Center, P Mark Hogarth, Amy W Chung, Stephen J Kent, Ivan Stratov
Studying HIV-infected individuals who control HIV replication (elite controllers, ECs) enable exploration of effective anti-HIV immunity. HIV Env- and non-Env-specific antibody-dependent cellular cytotoxicity (ADCC) may contribute to protection from progressive HIV infection but the evidence is limited. We recruited 22 ECs and matched them with 44 viremic subjects. HIV Env- and Vpu-specific ADCC responses were studied in sera using a novel ELISA-based dimeric recombinant soluble (rs) FcγRIIIa-binding assay, surface plasmon resonance, antibody-dependent natural killer (NK) cell activation assays and ADCC-mediated killing assays...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28699734/solution-and-solid-state-nmr-structural-investigations-of-the-antimicrobial-designer-peptide-gl13k-in-membranes
#16
Nicole Harmouche, Christopher Aisenbrey, Fernando Porcelli, Youlin Xia, Sarah E D Nelson, Xi Chen, Jesus Raya, Louic S Vermeer, Conrado Aparicio, Gianluigi Veglia, Sven-Ulrik Gorr, Burkhard Bechinger
The antimicrobial peptide GL13K encompasses 13 amino acid residues and has been designed and optimized from the salivary protein BPIFA2 to exhibit potent bacteriocidal and anti-biofilm activity against Gram-negative and Gram-positive bacteria as well as anti-lipopolysaccharide activity in vitro and in vivo. Here, the peptide was analyzed in a variety of membrane environments by CD spectroscopy and by high resolution multidimensional solution NMR spectroscopy. Whereas in the absence of membranes a random coil conformation predominates, the peptide adopts a helical structure from residues 5 to 11 in the presence of DPC micelles...
July 12, 2017: Biochemistry
https://www.readbyqxmd.com/read/28696251/endothelin-1-stimulates-vasoconstriction-through-rab11a-serine-177-phosphorylation
#17
Xue Zhai, Marie D Leo, Jonathan H Jaggar
Rationale: Large-conductance calcium (Ca(2+))-activated potassium channels (BK) are composed of pore-forming BKα and auxiliary β1 subunits in arterial smooth muscle cells (myocytes). Vasoconstrictors, including endothelin-1 (ET-1), inhibit myocyte BK channels, leading to contraction, but mechanisms involved are unclear. Recent evidence indicates that BKα is primarily plasma membrane-localized, whereas the cellular location of β1 can be rapidly altered by Rab11A-positive recycling endosomes. Whether vasoconstrictors regulate the multi-subunit composition of surface BK channels to stimulate contraction is unclear...
July 10, 2017: Circulation Research
https://www.readbyqxmd.com/read/28695856/crystal-structure-of-yersinia-pestis-virulence-factor-yfea-reveals-two-polyspecific-metal-binding-sites
#18
Christopher D Radka, Lawrence J DeLucas, Landon S Wilson, Matthew B Lawrenz, Robert D Perry, Stephen G Aller
Gram-negative bacteria use siderophores, outer membrane receptors, inner membrane transporters and substrate-binding proteins (SBPs) to transport transition metals through the periplasm. The SBPs share a similar protein fold that has undergone significant structural evolution to communicate with a variety of differentially regulated transporters in the cell. In Yersinia pestis, the causative agent of plague, YfeA (YPO2439, y1897), an SBP, is important for full virulence during mammalian infection. To better understand the role of YfeA in infection, crystal structures were determined under several environmental conditions with respect to transition-metal levels...
July 1, 2017: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/28695569/in-vitro-evidence-for-the-participation-of-drosophila-melanogaster-sperm-%C3%AE-n-acetylglucosaminidases-in-the-interactions-with-glycans-carrying-terminal-n-acetylglucosamine-residues-on-the-egg-s-envelopes
#19
Jari Intra, Concetta Veltri, Daniela De Caro, Maria Elisa Perotti, Maria Enrica Pasini
Fertilization is a complex and multiphasic process, consisting of several steps, where egg-coating envelope's glycoproteins and sperm surface receptors play a critical role. Sperm-associated β-N-acetylglucosaminidases, also known as hexosaminidases, have been identified in a variety of organisms. Previously, two isoforms of hexosaminidases, named here DmHEXA and DmHEXB, were found as intrinsic proteins in the sperm plasma membrane of Drosophila melanogaster. In the present work, we carried out different approaches using solid-phase assays in order to analyze the oligosaccharide recognition ability of D...
July 10, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28693188/consecutive-stimulation-of-hbsag-promotes-the-viability-of-the-human-b-lymphoblastoid-cell-line-im-9-through-regulating-the-sirt1-nf-%C3%AE%C2%BAb-pathway
#20
Jian Bo, Xiaojuan Wang, Jie Li, Wenqing Wang, Jinqian Zhang
Patients with chronic HBV infection have been reported to suffer a significantly increased risk of NHL, but the underlying mechanisms remain to be clearly explained. The aim of the present study was to clarify the relationship between chronic HBV infection and NHL development. Fluorescence-activated cell sorting, Annexin V/7-aminoactinomycin D staining and MTS assay were used to analyze the rate of apoptosis and cell viability. In addition, western blotting was used to detect protein expression. The effects of the activator of SIRT1, SRT1720, and the inhibitor of SIRT1, nicotinamide, were also analyzed...
July 2017: Oncology Letters
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