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https://www.readbyqxmd.com/read/29149301/use-of-domain-swapping-to-identify-candidate-amino-acids-involved-in-differential-interactions-between-two-allelic-variants-of-type-1%C3%A2-s-locus-f-box-protein-and-s3-rnase-in-petunia-inflata
#1
Lihua Wu, Justin S Williams, Ning Wang, Wasi A Khatri, Daniele San Román, Teh-Hui Kao
Petunia inflata possesses a self-incompatibility (SI) mechanism, which involves S-RNase and multiple S-locus F-box (SLF) genes at the polymorphic S-locus. For a given S-haplotype, each SLF is thought to interact with some of its non-self S-RNases, but not with its self-S-RNase. In this work, we studied an allelic pair of SLF1, S2-SLF1 and S3-SLF1, which differ in 44 amino acids and show differential interactions with S3-RNase. We first used an in vivo transgenic assay to determine whether four chimeric proteins of S2-SLF1 and S3-SLF1, each with one of the three functional domains swapped, interact with S3-RNase...
November 15, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29147713/variation-of-the-bone-forming-ability-with-the-physicochemical-properties-of-calcium-phosphate-bone-substitutes
#2
Rongquan Duan, Davide Barbieri, Xiaoman Luo, Jie Weng, Chongyun Bao, Joost D de Bruijn, Huipin Yuan
Because of their bioactive properties and chemical similarity to the inorganic component of bone, calcium phosphate (CaP) materials are widely used for bone regeneration. Six commercially available CaP bone substitutes (Bio-Oss, Actifuse, Bi-Ostetic, MBCP, Vitoss and chronOs) as well as two tricalcium phosphate (TCP) ceramics with either a micron-scale (TCP-B) or submicron-scale (TCP-S) surface structure are characterized and their bone forming potential is evaluated in a canine ectopic implantation model. After 12 weeks of implantation in the paraspinal muscle of four beagles, sporadic bone (0...
November 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/29145826/gpu-based-detection-of-protein-cavities-using-gaussian-surfaces
#3
Sérgio E D Dias, Ana Mafalda Martins, Quoc T Nguyen, Abel J P Gomes
BACKGROUND: Protein cavities play a key role in biomolecular recognition and function, particularly in protein-ligand interactions, as usual in drug discovery and design. Grid-based cavity detection methods aim at finding cavities as aggregates of grid nodes outside the molecule, under the condition that such cavities are bracketed by nodes on the molecule surface along a set of directions (not necessarily aligned with coordinate axes). Therefore, these methods are sensitive to scanning directions, a problem that we call cavity ground-and-walls ambiguity, i...
November 16, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/29143459/the-spatial-patterning-of-rgd-and-bmp-2-mimetic-peptides-at-the-subcellular-scale-modulates-human-mesenchymal-stem-cells-osteogenesis
#4
I Bilem, L Plawinski, P Chevallier, C Ayela, E D Sone, G Laroche, M C Durrieu
Engineering artificial extracellular matrices (ECMs), based on the biomimicry of the spatial distribution of proteins and growth factors within their native microenvironment, is of great importance for understanding mechanisms of bone tissue regeneration. Herein, photolithography is used to decorate glass surfaces with subcellular patterns of RGD and BMP-2 ligands; two mimetic peptides recognized to be involved in stem cells osteogenesis. The biological relevance of well-defined RGD and BMP-2 patterned surfaces is evaluated by investigating the differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts, in the absence of induction media...
November 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29142162/chloroform-extract-of-turmeric-inhibits-biofilm-formation-eps-production-and-motility-in-antibiotic-resistant-bacteria
#5
Sumreen Hayat, Anjum N Sabri, Timothy D McHugh
In the form of biofilms, bacteria exhibit more resistance to antibiotics. Biofilm formers can withstand severe conditions and the host's defense system. Therefore, it is necessary to search for effective biofilm inhibitors. In this study, we investigated the effect of a chloroform extract of turmeric on biofilm formation against antibiotic resistant bacteria. The extract exhibited its antibiofilm effect by altering adherence, motility, extracellular polymeric substance (EPS) production and cell surface hydrophobicity; important attributes of biofilm formation...
November 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29141912/wh2-and-proline-rich-domains-of-wasp-family-proteins-collaborate-to-accelerate-actin-filament-elongation
#6
Peter Bieling, Scott D Hansen, Orkun Akin, Tai-De Li, Carl C Hayden, Daniel A Fletcher, R Dyche Mullins
WASP-family proteins are known to promote assembly of branched actin networks by stimulating the filament-nucleating activity of the Arp2/3 complex. Here, we show that WASP-family proteins also function as polymerases that accelerate elongation of uncapped actin filaments. When clustered on a surface, WASP-family proteins can drive branched actin networks to grow much faster than they could by direct incorporation of soluble monomers. This polymerase activity arises from the coordinated action of two regulatory sequences: (i) a WASP homology 2 (WH2) domain that binds actin, and (ii) a proline-rich sequence that binds profilin-actin complexes...
November 15, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29138425/timescale-separation-of-positive-and-negative-signaling-creates-history-dependent-responses-to-ige-receptor-stimulation
#7
Brooke Harmon, Lily A Chylek, Yanli Liu, Eshan D Mitra, Avanika Mahajan, Edwin A Saada, Benjamin R Schudel, David A Holowka, Barbara A Baird, Bridget S Wilson, William S Hlavacek, Anup K Singh
The high-affinity receptor for IgE expressed on the surface of mast cells and basophils interacts with antigens, via bound IgE antibody, and triggers secretion of inflammatory mediators that contribute to allergic reactions. To understand how past inputs (memory) influence future inflammatory responses in mast cells, a microfluidic device was used to precisely control exposure of cells to alternating stimulatory and non-stimulatory inputs. We determined that the response to subsequent stimulation depends on the interval of signaling quiescence...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29138085/in-silico-design-cloning-expression-and-immunologic-evaluation-of-ed-fusion-protein-of-nt-h-influenzae
#8
Ava Behrouzi, Saeid Bouzari, Mana Oloomi, Abolfaz Fateh, Farzam Vaziri, Parviz Afrough, Amir Amanzadeh, Seyed Davar Siadat
Infections due to nontypeable Haemophilus influenzae (NTHi) are important causes of child mortality throughout the world. Given the lack of effective vaccines for these strains and the spread and prevalence of these infections in the world, it is necessary to design novel vaccine candidates against these strains. D and E proteins are conserved membrane-specific lipoproteins among encapsulated and non-encapsulated H. influenza strains, which, according to the exposure surface and conservation degree between both strains, can be considered as vaccine candidates suitable for studies...
November 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29135238/elucidating-protein-ligand-recognition-with-combined-surface-plasmon-resonance-and-surface-enhanced-raman-spectroscopy
#9
Ju-Young Kim, Zhi-Cong Zeng, Lifu Xiao, Zachary D Schultz
The ability to distinguish between specific and non-specific binding is important for assessing the interactions between protein receptors and ligands. Surface plasmon resonance (SPR) spectroscopy is an advanced tool to measure the binding events, yet the ability to distinguish between specific and non-specific binding remains a limitation. To address this problem, we use SPR spectroscopy correlated with surface enhanced Raman scattering (SERS). The chemical information present in SERS spectra provides insight into the molecular interactions between functionalized nanoparticles and proteins, which are not detectable by SPR alone...
November 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29133883/pseudomonas-aeruginosa-defends-against-phages-through-type-iv-pilus-glycosylation
#10
Hanjeong Harvey, Joseph Bondy-Denomy, Hélène Marquis, Kristina M Sztanko, Alan R Davidson, Lori L Burrows
Since phages present a major challenge to survival in most environments, bacteria express a battery of anti-phage defences including CRISPR-Cas, restriction-modification and abortive infection systems (1-4) . Such strategies are effective, but the phage genome-which encodes potentially inhibitory gene products-is still allowed to enter the cell. The safest way to preclude phage infection is to block initial phage adsorption to the cell. Here, we describe a cell-surface modification that blocks infection by certain phages...
November 13, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/29133563/a-promising-anti-prion-trimethoxychalcone-binds-to-the-globular-domain-of-prp-c-and-changes-its-cellular-location
#11
N C Ferreira, L M Ascari, A G Hughson, G R Cavalheiro, C F Góes, P N Fernandes, J R Hollister, R A da Conceição, D S Silva, A M T Souza, M L C Barbosa, F A Lara, R A P Martins, B Caughey, Y Cordeiro
The search for anti-prion compounds has been encouraged by the fact that transmissible spongiform encephalopathies (TSEs) share molecular mechanisms with more prevalent neurodegenerative pathologies, such as Parkinson's and Alzheimer's diseases. Cellular prion protein conversion into protease-resistant forms (PrP(Res) or PrP(Sc)) is a critical step in the development of TSEs, thus being one of the main targets in the screening for anti-prion compounds. In this work, three trimethoxychalcones (J1, J8, J20) and one oxadiazole (Y17), previously identified in vitro as potential anti-prion compounds, were evaluated through different approaches in order to gain inferences about their mechanism of action...
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29132840/d-peptides-as-inhibitors-of-pr3-membrane-interactions
#12
Ksenia Maximova, Tom Venken, Nathalie Reuter, Joanna Trylska
Proteinase 3 (PR3) is a neutrophil serine protease present in cytoplasmic granules but also expressed at the neutrophil surface where it mediates proinflammatory effects. Studies of the underlying molecular mechanisms have been hampered by the lack of inhibitors of the PR3 membrane anchorage. Indeed while there exist inhibitors of the catalytic activity of PR3, its membrane interfacial binding site (IBS) is distinct from its catalytic site. The IBS has been characterized both by mutagenesis experiments and molecular modeling...
November 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29132041/preparation-of-a-beta-tricalcium-phosphate-nanocoating-and-its-protein-adsorption-behaviour-by-quartz-crystal-microbalance-with-dissipation-technique
#13
Dongwen Pang, Liyong He, Luxi Wei, Huade Zheng, Chunlin Deng
Beta-tricalcium phosphate (β-TCP) nanocoatings, which can be analysed using a quartz crystal microbalance with dissipation technique (QCM-D), were fabricated on a gold surface by electrophoretic deposition. The influences of electric field intensity and electrophoresis time were investigated. The adsorption behaviours of bovine serum albumin (BSA) and lysozyme (LSZ) on Au and β-TCP surfaces were observed in real time by QCM-D. The homogeneous β-TCP nanocoating with moderately sized particles on gold surface was fabricated at 25V/cm for 5min, and it met the requirements for the QCM-D experiment...
November 8, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29131831/substrate-specificity-of-human-metallocarboxypeptidase-d-comparison-of-the-two-active-carboxypeptidase-domains
#14
Javier Garcia-Pardo, Sebastian Tanco, Lucía Díaz, Sayani Dasgupta, Juan Fernandez-Recio, Julia Lorenzo, Francesc X Aviles, Lloyd D Fricker
Metallocarboxypeptidase D (CPD) is a membrane-bound component of the trans-Golgi network that cycles to the cell surface through exocytic and endocytic pathways. Unlike other members of the metallocarboxypeptidase family, CPD is a multicatalytic enzyme with three carboxypeptidase-like domains, although only the first two domains are predicted to be enzymatically active. To investigate the enzymatic properties of each domain in human CPD, a critical active site Glu in domain I and/or II was mutated to Gln and the protein expressed, purified, and assayed with a wide variety of peptide substrates...
2017: PloS One
https://www.readbyqxmd.com/read/29129382/the-cryoelectron-microscopy-structure-of-the-type-1-chaperone-usher-pilus-rod
#15
Manuela K Hospenthal, Dawid Zyla, Tiago R D Costa, Adam Redzej, Christoph Giese, James Lillington, Rudi Glockshuber, Gabriel Waksman
Adhesive chaperone-usher pili are long, supramolecular protein fibers displayed on the surface of many bacterial pathogens. The type 1 and P pili of uropathogenic Escherichia coli (UPEC) play important roles during urinary tract colonization, mediating attachment to the bladder and kidney, respectively. The biomechanical properties of the helical pilus rods allow them to reversibly uncoil in response to flow-induced forces, allowing UPEC to retain a foothold in the unique and hostile environment of the urinary tract...
November 7, 2017: Structure
https://www.readbyqxmd.com/read/29127342/chemical-analysis-of-morphological-changes-in-lysophosphatidic-acid-treated-ovarian-cancer-cells
#16
Karen A Bailey, Yuliya Klymenko, Peter E Feist, Amanda B Hummon, M Sharon Stack, Zachary D Schultz
Ovarian cancer (OvCa) cells are reported to undergo biochemical changes at the cell surface in response to treatment with lysophosphatidic acid (LPA). Here we use scanning electron microscopy (SEM) and multiplex coherent anti-Stokes Raman scattering (CARS) imaging via supercontinuum excitation to probe morphological changes that result from LPA treatment. SEM images show distinct shedding of microvilli-like features upon treatment with LPA. Analysis of multiplex CARS images can distinguish between molecular components, such as lipids and proteins...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29127147/igg1-is-required-for-optimal-protection-after-immunization-with-the-purified-porin-ompd-from-salmonella-typhimurium
#17
Yang Zhang, Coral Dominguez-Medina, Nicola J Cumley, Jennifer N Heath, Sarah J Essex, Saeeda Bobat, Anna Schager, Margaret Goodall, Sven Kracker, Christopher D Buckley, Robin C May, Robert A Kingsley, Calman A MacLennan, Constantino López-Macías, Adam F Cunningham, Kai-Michael Toellner
In mice, the IgG subclass induced after Ag encounter can reflect the nature of the Ag. Th2 Ags such as alum-precipitated proteins and helminths induce IgG1, whereas Th1 Ags, such as Salmonella Typhimurium, predominantly induce IgG2a. The contribution of different IgG isotypes to protection against bacteria such as S. Typhimurium is unclear, although as IgG2a is induced by natural infection, it is assumed this isotype is important. Previously, we have shown that purified S. Typhimurium porins including outer membrane protein OmpD, which induce both IgG1 and IgG2a in mice, provide protection to S...
November 10, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29127068/mono-vs-multilayer-fibronectin-coatings-on-polar-hydrophobic-ionic-polyurethanes-altering-surface-interactions-with-human-monocytes
#18
Audrey Gossart, Kyle G Battiston, Adeline Gand, Emmanuel Pauthe, J Paul Santerre
Monocyte interactions with materials that are biofunctionalized with fibronectin (Fn) are of interest because of the documented literature which associates this protein with white blood cell function at implant sites. A degradable-polar hydrophobic ionic polyurethane (D-PHI), has been reported to promote an anti-inflammatory response from human monocytes. The aim of the current work was to study the influence of intrinsic D-PHI material chemistry on Fn adsorption (mono and multi-layer structures), and to investigate the influence of such chemistry on the structural state of the Fn, as well as the latter's influence on the activity of human monocytes on the protein coated substrates...
November 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29127055/fractionation-separation-of-human-plasma-proteins-using-hplc-with-a-homemade-iron-porphyrin-based-monolithic-column
#19
Doudou Zhang, Yu Zhao, Dandan Lan, Xiaomin Pang, Ligai Bai, Haiyan Liu, Hongyuan Yan
In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50×4.6mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N,N-dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis, scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29126905/failure-of-active-targeting-by-a-cholesterol-anchored-ligand-and-improvement-by-altering-the-lipid-composition-to-prevent-ligand-desorption
#20
Shoshiro Yamamoto, Yu Sakurai, Hideyoshi Harashima
Although anti-angiogenic therapy is predicted to be an effective therapy for treating cancer, selectively targeting tumor endothelial cells (TECs), and not normal endothelial cells, remains a major obstacle. Modifying a drug carrier with a targeting ligand is a popular strategy for developing an active-targeting type drug delivery system (DDS). We previously reported that a cyclo(Arg-Gly-Asp-D-Phe-Lys) (cRGD)-equipped liposome that contains encapsulated siRNA (RGD-MEND) achieved an efficient therapeutic outcome in a murine cancer model...
November 7, 2017: International Journal of Pharmaceutics
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