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Kai Li, Yue Zhang, Yuwei Zhang, Wenqing Jiang, Junhui Shen, Song Xu, Daozhang Cai, Jie Shen, Bin Huang, Mangmang Li, Qiancheng Song, Yu Jiang, Anling Liu, Xiaochun Bai
OBJECTIVES: To investigate the role of tyrosine kinase Fyn in the development of osteoarthritis (OA) and the underlying mechanisms, and to define whether targeting Fyn could prevent OA in mice. METHODS: Cartilage samples from normal and aged mice were analysed with proteome-wide screening. Fyn expression was examined with immunofluorescence in human and age-dependent or experimental mouse OA cartilage samples. Experimental OA in Fyn-knockout mice was induced by destabilisation of the medial meniscus...
March 19, 2018: Annals of the Rheumatic Diseases
Mina Aghsoleimani, Hamid Jalilvand, Mohammad Ebrahim Mahdavi, Ahmad Reza Nazeri, Mohammad Kamali
Objectives: Directional microphone technology can enhance the speech intelligibility and listening comfort of listeners with hearing impairment. The main aim of this study is to investigate and compare the benefit derived by listeners with severe hearing loss from directional microphone technology with that derived by listeners with moderate hearing loss. Methods: The acceptable noise levels (ANLs) of two groups of listeners with moderate or severe hearing impairment (17 subjects in each group) were measured under unaided, omnidirectional-baseline-aided, and directional-aided conditions...
March 10, 2018: Clinical and Experimental Otorhinolaryngology
Xi Liu, Ping Du, Lei Han, Anling Zhang, Kui Jiang, Qingyu Zhang
Gastric cancer is one of the most common cancers in the world; taxol displayed modest efficacy as first-line chemotherapy for gastric cancer, conversely, it has limitations used alone. β-catenin is a multifunctional oncogenic protein and the elevation in expression and activity of β-catenin has been implicated in many cancers. Therefore, we assume that the inhibition of β-catenin can enhanced the efficacy of taxol. The purpose of this study was to investigate the inhibitory effect of miR-200a mimics, FH535 combined with taxol on proliferation and invasion of human gastric cancer cell lines SGC-7901 and BGC-823...
December 6, 2017: Pathology, Research and Practice
María Graciela Delgado, Carlos Oliva, Estefanía López, Andrés Ibacache, Alex Galaz, Ricardo Delgado, L Felipe Barros, Jimena Sierralta
The intercellular transport of lactate is crucial for the astrocyte-to-neuron lactate shuttle (ANLS), a model of brain energetics according to which neurons are fueled by astrocytic lactate. In this study we show that the Drosophila chaski gene encodes a monocarboxylate transporter protein (MCT/SLC16A) which functions as a lactate/pyruvate transporter, as demonstrated by heterologous expression in mammalian cell culture using a genetically encoded FRET nanosensor. chaski expression is prominent in the Drosophila central nervous system and it is particularly enriched in glia over neurons...
January 19, 2018: Scientific Reports
Jikui Sun, Zhifan Jia, Banban Li, Anling Zhang, Guangxiu Wang, Peiyu Pu, Zhijuan Chen, Zengguang Wang, Weidong Yang
Accumulating data demonstrates that the network dysregulation of microRNA-medicated target genes is involved in glioma. We have previously found miR-19a/b overexpression in glioma cell lines and specimens with various tumour grades. However, there was no report on the function and regulatory mechanism of miR-19a/b in glioma. In this study, based on our previous research data, we first determine the inverse relationship between miR-19 (miR-19a and miR-19b) and RUNX3 which is also identified the reduced expression in tumour tissues by real-time PCR and IHC...
December 19, 2017: Oncotarget
Jana Broecker, Takefumi Morizumi, Wei-Lin Ou, Viviane Klingel, Anling Kuo, David J Kissick, Andrii Ishchenko, Ming-Yue Lee, Shenglan Xu, Oleg Makarov, Vadim Cherezov, Craig M Ogata, Oliver P Ernst
Protein crystallography has significantly advanced in recent years, with in situ data collection, in which crystals are placed in the X-ray beam within their growth medium, being a major point of focus. In situ methods eliminate the need to harvest crystals, a previously unavoidable drawback, particularly for often small membrane-protein crystals. Here, we present a protocol for the high-throughput in situ X-ray screening of and data collection from soluble and membrane-protein crystals at room temperature (20-25°C) and under cryogenic conditions...
February 2018: Nature Protocols
Bor Luen Tang
The astrocyte-neuron lactate shuttle (ANLS) hypothesis posits that during neuronal activation, astrocytic glycolysis consumes glucose and generates lactate, with the latter then imported by neurons as a preferred fuel. The hypothesis has been controversial, with multiple theoretical postulates for and against, and with empirical evidence that were either supportive or otherwise. Recent findings using direct in vivo imaging of lactate and glucose uptake as well as associated metabolic changes in neurons have now placed important constraints on the hypothesis...
December 19, 2017: Brain Research Bulletin
Liang Kong, Yan Zhao, Wen-Juan Zhou, Hui Yu, Shuai-Wen Teng, Qi Guo, Zheyu Chen, Yue Wang
The metabolism of glucose is a nearly exclusive source of energy for maintaining neuronal survival, synaptic transmission and information processing in the brain. Two glucose metabolism pathways have been reported, direct neuronal glucose uptake and the astrocyte-neuron lactate shuttle (ANLS), which can be involved in these functions simultaneously or separately. Although ANLS in the dorsal hippocampus (DH) has been proved to be required for memory consolidation, the specific metabolic pathway involved during memory acquisition remains unclear...
2017: Frontiers in Molecular Neuroscience
Yongkui Wang, Min Xiao, Chen Tao, Jing Chen, Zhenyu Wang, Jun Yang, Zhenguo Chen, Zhipeng Zou, Anling Liu, Daozhang Cai, Yu Jiang, Changhai Ding, Mangmang Li, Xiaochun Bai
Osteoblasts provide a microenvironmental niche for B-cell commitment and maturation in the bone marrow (BM). Any abnormity of osteoblasts function may result in the defect of B lymphopoiesis. Signaling from mechanistic target of rapamycin complex 1 (mTORC1) has been implicated in regulating the expansion and differentiation of osteoblasts. Thus, we raise a hypothesis that mTORC1 signaling in osteoblasts plays a vital role in B-cell development. Inactivation of mTORC1 in osterix-expressing cells (mainly osteoblast lineage) through Osx-Cre-directed deletion of Raptor (an mTORC1-specific component) resulted in a reduction in the total B-cell population in the BM, which was due to a block in early B-cell development from the pro-B to pre-B cell stage...
December 5, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
Julia M Goodman
Introduction Most women in the U.S. are employed during pregnancy and work until the month of childbirth. For many, working throughout pregnancy poses little threat to their health; however, women experiencing difficult pregnancies and/or working in strenuous or inflexible jobs may benefit from taking time from work as they approach childbirth, but almost no empirical evidence examines antenatal leave (ANL). Methods Using a national survey of English-speaking women, this paper offers the first national description of ANL and examines state policy predictors of uptake...
November 9, 2017: Maternal and Child Health Journal
J Gertjan Dingemanse, André Goedegebure
OBJECTIVE: ClearVoice is a single-microphone noise reduction algorithm in Advanced Bionics cochlear implant(CI) systems with the aim to improve performance in background noise. The present study investigated a hypothesised increased effect of ClearVoice if combined with a structural increase of maximum comfort stimulation levels (M-levels) in the CI fitting. DESIGN: We tested performance with ClearVoice (Medium) in four conditions, defined by combined settings of ClearVoice off/on and with/without 5% increase of M-levels...
October 24, 2017: International Journal of Audiology
Jie Peng, Yinghui Wu, Zhao Deng, Yuanfei Zhou, Tongxing Song, Yang Yang, Xiaming Zhang, Tao Xu, Mao Xia, Anle Cai, Zuhong Liu, Jian Peng
Obesity is associated with a wide range of metabolic disorders including inflammation and insulin-resistance. Sirtuin-1 (SIRT1) is an important regulator of metabolic homeostasis and stress response pathways in white adipose tissue. However, involvement of microRNAs (miRNAs) in regulating SIRT1 during obesity-induced inflammation and insulin-resistance remains unclear. Here, we found that miR-377 was upregulated in adipose tissue and showed a negative correlation with SIRT1 in chronic high fat diet (HFD)-fed mice...
September 19, 2017: Oncotarget
Cuihong Jin, Liang Gao, Yingqi Li, Shengwen Wu, Xiaobo Lu, Jinghua Yang, Yuan Cai
Rare-earth elements (REEs) are applied in various fields by virtue of their superior physical and chemical properties. Surveys have reported that REEs can impair learning and memory in children and induce neurobehavioral abnormalities in animals. However, the mechanism underlying this neurotoxicity is still unclear. Lanthanum (La) is often chosen to study the effects of REEs. Here, we investigated the role of astrocyte-neuron lactate shuttle (ANLS) in spatial learning and memory impairment induced by LaCl3 in hippocampus, an important spatial memory-related brain region...
December 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
Eric Boakye-Gyasi, Ella Anle Kasanga, Elvis Ofori Ameyaw, Wonder Kofi Mensah Abotsi, Robert Peter Biney, Christian Agyare, Eric Woode
BACKGROUND: Geraniin, a dehydroellagitannin, is a major component of the aqueous extract of the aerial parts of Phyllanthus muellerianus (Kuntze) Exell. (Euphorbiaceae). Several Phyllanthus species are traditionally used for painful disorders. The anti-nociceptive effects of the aqueous extract of the aerial parts of P. muellerianus and of geraniin have been scientifically established. The aim of the paper is to determine whether a combination of geraniin and diclofenac or geraniin and morphine leads to better anti-nociceptive effects...
October 9, 2017: Journal of Basic and Clinical Physiology and Pharmacology
Clare C Rittschof, Stefanie Schirmeier
Neuronal activity requires a vast amount of energy. Energy use in the brain is spatially and temporally dynamic, which reflects the changing activity of the neuronal circuits and might be important for modulating neuronal output. Much recent work has focused on understanding how brain glial cells take up nutrients from circulation and subsequently provide metabolic precursors to neurons. However, within the neurons, modulation of cellular metabolic pathway flux also regulates excitability and signaling. A coherent understanding of the links between energy availability and metabolism, neural signaling, and higher-level phenotypes like behavior requires a synthesis of the understanding of glial and neuronal metabolic dynamics...
September 29, 2017: Glia
Yan Liu, Michael J Conboy, Melod Mehdipour, Yutong Liu, Thanhtra P Tran, Aaron Blotnick, Prasanna Rajan, Thalie Cavalcante Santos, Irina M Conboy
Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in transplanted myoblasts, but not in host tissue, and proteins exclusive to one young mouse and transferred during parabiosis to its old partner. We use a transgenic mouse strain that ubiquitously expresses a modified tRNA methionine synthase, metRS, which preferentially incorporates the methionine surrogate azido-nor-leucine (ANL) into newly generated proteins...
September 21, 2017: Nature Communications
Hitarth B Bhatt, M Azmatunnisa Begum, Sasikala Chintalapati, Venkata Ramana Chintalapati, Satya P Singh
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
Manas Ranjan Saha, Priyankar Dey, Indrani Sarkar, Dilip De Sarker, Biswajit Haldar, Tapas Kumar Chaudhuri, Arnab Sen
ETHNOPHARMACOLOGICAL RELEVANCE: Acacia nilotica (L.) Delile is used as a traditional anti-diabetic remedy in Bangladesh, Pakistan, Egypt, Nigeria and is mentioned in Ayurveda as well. AIM: The objective of the study was to evaluate the ethnomedicinal claim of A. nilotica leaf (ANL) extract for its efficiency in ameliorating diabetic complications. MATERIALS AND METHODS: ANL was orally administrated (50 and 200mg/kg) to alloxanized mice (blood glucose > 200mg/dL) for 20d...
August 30, 2017: Journal of Ethnopharmacology
Luo Tian, Wen-Zuo Zhou, Wan-Hua He, Dan Lun, Dong-Hong Liu
"Adversity" is one primary element that impacts the pharmacology components of authentic Chinese herbal medicine. Knowledge about "adversity" is a precondition of yield estimation, quality monitoring, location selection and the geo-herbalism protection. Used 3S(GIS, RS and GPS) technology to combine multi-source key ecological factors of "Anling", and used parasitic relationships between organisms to extracting its suitable region for the first time. Results showed that the "Anling" were mainly distributed in Dabie coteau...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Houcheng Liang, Jingdong Zhang, Pifu Luo, Hongna Zhu, Ying Qiao, Anle Su, Ting Zhang
The objective of this study was to explore whether there was a functional link between trigeminal proprioception and the oculomotor system mediated through jaw muscle afferents. Electromyography (EMG) was undertaken of the levator palpebrae (LP) and superior rectus (SR), and Fos expression was detected in the brainstem following consecutive down-stretching of the lower jaw at 2-4 Hz in rats. Retrograde tracing was undertaken of the interstitial nucleus of Cajal and Darkschewitsch nucleus (INC/DN) pre-oculomotor neurons...
January 19, 2017: Journal of Biomedical Research
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