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https://www.readbyqxmd.com/read/28261000/a-versatile-system-for-high-throughput-in-situ-x-ray-screening-and-data-collection-of-soluble-and-membrane-protein-crystals
#1
Jana Broecker, Viviane Klingel, Wei-Lin Ou, Aidin R Balo, David J Kissick, Craig M Ogata, Anling Kuo, Oliver P Ernst
In recent years, in situ data collection has been a major focus of progress in protein crystallography. Here, we introduce the Mylar in situ method using Mylar-based sandwich plates that are inexpensive, easy to make and handle, and show significantly less background scattering than other setups. A variety of cognate holders for patches of Mylar in situ sandwich films corresponding to one or more wells makes the method robust and versatile, allows for storage and shipping of entire wells, and enables automated crystal imaging, screening, and goniometer-based X-ray diffraction data-collection at room temperature and under cryogenic conditions for soluble and membrane-protein crystals grown in or transferred to these plates...
November 2, 2016: Crystal Growth & Design
https://www.readbyqxmd.com/read/28259411/genetic-and-functional-analysis-of-the-bovine-uterine-microbiota-part-ii-purulent-vaginal-discharge-versus-healthy-cows
#2
M L S Bicalho, S Lima, C H Higgins, V S Machado, F S Lima, R C Bicalho
The aim of this study was to characterize, using metagenomic shotgun DNA sequencing, the intrauterine microbial population and its predicted functional diversity within healthy cows and cows presenting purulent vaginal discharge (PVD). Twenty Holstein dairy cows from a single farm were enrolled in the study at 25 to 35 d postpartum. Purulent vaginal discharge was diagnosed by retrieving and scoring vaginal discharge using the Metricheck device (Simcro, Hamilton, New Zealand). Intrauterine samples for metagenomic analysis were collected by the cytobrush technique from 8 cows diagnosed with PVD and 12 healthy cows...
March 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28259404/genetic-and-functional-analysis-of-the-bovine-uterine-microbiota-part-i-metritis-versus-healthy-cows
#3
M L S Bicalho, V S Machado, C H Higgins, F S Lima, R C Bicalho
Metritis is a uterine disease that affects 10 to 30% of all lactating dairy cows and has detrimental effects on reproductive performance, milk production, and survival. Data regarding the identity and abundance of bacterial genes governing traits such as virulence, antibiotic resistance, and stress responses could enable identification of previously unknown agents that play a role in metritis pathogenesis. Moreover, such knowledge could lead to the development of improved treatments or preventive methods. Therefore, the objectives of this study were to characterize the uterine microbial population and to differentiate, for the first time, the microbial functional diversity in cows with metritis versus healthy cows...
March 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28251459/lycorine-inhibits-breast-cancer-growth-and-metastasis-via-inducing-apoptosis-and-blocking-src-fak-involved-pathway
#4
Xuexiang Ying, Anling Huang, Yajing Xing, Liping Lan, Zhengfang Yi, Pingqing He
Breast cancer is the most commonly diagnosed cancer type worldwide among women and more than 90% of patients die from tumor metastasis. Lycorine, a natural alkaloid, has been widely reported possessing potential efficacy against cancer proliferation and metastasis. In our study, the anti-tumor potency on breast cancer was evaluated in vitro and in vivo for the first time. Our results indicated that lycorine inhibited breast cancer cells growth, migration and invasion as well as induced their apoptosis. In in vivo study, lycorine not only suppressed breast tumor growth in xenograft models and inhibited breast tumor metastasis in MDA-MB-231 tail vein model...
February 27, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28244316/highly-efficient-enzymatic-acylation-of-dihydromyricetin-by-the-immobilized-lipase-with-deep-eutectic-solvents-as-cosolvent
#5
Shi-Lin Cao, Xiao Deng, Pei Xu, Zi-Xuan Huang, Jian Zhou, Xue-Hui Li, Min-Hua Zong, Wen-Yong Lou
A novel deep eutectic solvent (DES)-DMSO cosolvent system has been, for the first time, successfully used as the reaction medium for the enzymatic acylation of dihydromyricetin (DMY) catalyzed by the immobilized lipase from Aspergillus niger (ANL). The cosolvent mixture, ChCl:Glycerol-DMSO (1:3, v/v) proved to be the optimal medium. With the newly developed cosolvent, the initial reaction rate of enzymatic acylation of DMY achieved 11.1 mM/h and the conversion of DMY was 91.6%. ANL@PD-MNPs is stable and recyclable in this cosolvent, offering 90% conversion rate after repeated use of 5 times...
March 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28169839/speech-intelligibility-as-a-cue-for-acceptable-noise-levels
#6
Karrie L Recker, Christophe Micheyl
OBJECTIVES: The goal of this study was to examine whether individuals are using speech intelligibility to determine how much noise they are willing to accept while listening to running speech. Previous research has shown that the amount of background noise that an individual is willing to accept while listening to speech is predictive of his or her likelihood of success with hearing aids. If it were possible to determine the criterion by which individuals make this judgment, then it may be possible to alter this cue, especially for those who are unlikely to be successful with hearing aids, and thereby improve their chances of success with hearing aids...
February 6, 2017: Ear and Hearing
https://www.readbyqxmd.com/read/28110956/anti-hiv-diphyllin-glycosides-from-justicia-gendarussa
#7
Hong-Jie Zhang, Emily Rumschlag-Booms, Yi-Fu Guan, Kang-Lun Liu, Dong-Ying Wang, Wan-Fei Li, Van Hung Nguyen, Nguyen Manh Cuong, Djaja Doel Soejarto, Harry H S Fong, Lijun Rong
In a search for new anti-HIV active leads from over several thousands of plant extracts, we have identified a potent plant lead. The active plant is determined as Justicia gendarussa (Acanthaceae), a medicinal plant that has been used for the treatment of injury, arthritis and rheumatism in Asia including China. Our bioassay-guided fractionation of the methanol extract of the stems and barks of the plant led to the isolation of two anti-HIV compounds, justiprocumins A and B. The compounds are identified as new arylnaphthalide lignans (ANL) glycosides...
April 2017: Phytochemistry
https://www.readbyqxmd.com/read/28057680/an-informative-approach-on-differential-abundance-analysis-for-time-course-metagenomic-sequencing-data
#8
Dan Luo, Sara Ziebell, Lingling An
MOTIVATION: The advent of high-throughput next generation sequencing technology has greatly promoted the field of metagenomics where previously unattainable information about microbial communities can be discovered. Detecting differentially abundant features (e.g., species or genes) plays a critical role in revealing the contributors (i.e., pathogens) to the biological or medical status of microbial samples. However, currently available statistical methods lack power in detecting differentially abundant features contrasting different biological or medical conditions, in particular, for time series metagenomic sequencing data...
January 5, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28036411/the-acceptable-noise-level-and-the-pure-tone-audiogram
#9
K Jonas Brännström, Steen Østergaard Olsen
Purpose: The vast majority of previous studies suggest that there is no relationship between the acceptable noise level (ANL) and pure-tone hearing thresholds reported as the average pure-tone hearing thresholds (pure-tone average). This study aims to explore (a) the relationship between hearing thresholds at individual frequencies and the ANL and (b) a measure of the slope of the audiogram and ANL. Method: Sixty-three Danish adult hearing aid users participated...
March 1, 2017: American Journal of Audiology
https://www.readbyqxmd.com/read/27987117/differential-effects-of-type-2-diabetes-on-brain-glycometabolism-in-rats-focus-on-glycogen-and-monocarboxylate-transporter-2
#10
Takeru Shima, Subrina Jesmin, Takashi Matsui, Mariko Soya, Hideaki Soya
Astrocyte-neuron lactate shuttle (ANLS) is a pathway that supplies glycogen-derived lactate to active neurons via monocarboxylate transporter 2 (MCT2), and is important for maintaining brain functions. Our study revealed alterations of ANLS with hippocampal hyper-glycogen levels and downregulated MCT2 protein levels underlying hippocampal dysfunctions as a complication in type 2 diabetic (T2DM) animals. Since T2DM rats exhibit brain dysfunctions involving several brain regions, we examined whether there might also be T2DM effects on ANLS's disturbances in other brain loci...
December 16, 2016: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/27854309/qsar-study-for-carcinogenic-potency-of-aromatic-amines-based-on-gep-and-mlps
#11
Fucheng Song, Anling Zhang, Hui Liang, Lianhua Cui, Wenlian Li, Hongzong Si, Yunbo Duan, Honglin Zhai
A new analysis strategy was used to classify the carcinogenicity of aromatic amines. The physical-chemical parameters are closely related to the carcinogenicity of compounds. Quantitative structure activity relationship (QSAR) is a method of predicting the carcinogenicity of aromatic amine, which can reveal the relationship between carcinogenicity and physical-chemical parameters. This study accessed gene expression programming by APS software, the multilayer perceptrons by Weka software to predict the carcinogenicity of aromatic amines, respectively...
November 15, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27808657/the-fractal-characteristic-of-facial-anthropometric-data-for-developing-pca-fit-test-panels-for-youth-born-in-central-china
#12
Lei Yang, Ran Wei, Henggen Shen
New principal component analysis (PCA) respirator fit test panels had been developed for current American and Chinese civilian workers based on anthropometric surveys. The PCA panels used the first two principal components (PCs) obtained from a set of 10 facial dimensions. Although the PCA panels for American and Chinese subjects adopted the bivairate framework with two PCs, the number of the PCs retained in the PCA analysis was different between Chinese subjects and Americans. For the Chinese youth group, the third PC should be retained in the PCA analysis for developing new fit test panels...
January 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/27773528/antenatal-maternity-leave-and-childbirth-using-the-first-baby-study-a-propensity-score-analysis
#13
Julia M Goodman, Sylvia Guendelman, Kristen H Kjerulff
BACKGROUND: Most employed American women work during pregnancy and continue working through the month they deliver. Yet, few studies estimate the relationship between maternity leave taken during pregnancy and maternal health. We evaluate the association of antenatal leave (ANL) uptake with obstetric outcomes, assessing the potential role of protective and adverse selection pathways on this relationship. METHODS: We sample 1,740 employed women who delivered at term from the First Baby Study, a prospective cohort of nulliparous women in Pennsylvania...
January 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/27768076/measurement-of-x-ray-beam-coherence-along-multiple-directions-using-2-d-checkerboard-phase-grating
#14
Shashidhara Marathe, Xianbo Shi, Michael J Wojcik, Albert T Macrander, Lahsen Assoufid
A procedure for a technique to measure the transverse coherence of synchrotron radiation X-ray sources using a single phase grating interferometer is reported. The measurements were demonstrated at the 1-BM bending magnet beamline of the Advanced Photon Source (APS) at Argonne National Laboratory (ANL). By using a 2-D checkerboard π/2 phase-shift grating, transverse coherence lengths were obtained along the vertical and horizontal directions as well as along the 45° and 135° directions to the horizontal direction...
October 11, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27764783/the-role-of-transcriptional-coactivator-taz-in-gliomas
#15
Weijie Li, Shicai Dong, Wei Wei, Guangxiu Wang, Anling Zhang, Peiyu Pu, Zhifan Jia
The transcriptional coactivator with PDZ-binding motif (TAZ) is one of the important downstream effectors of Hippo pathway. In this study, the potential implication of TAZ in gliomagenesis was explored. TAZ expression was identified to be upregulated in glioma specimens and positively correlated with tumor grade. Meanwhile, its expression in nucleus was increased more significantly with the ascending order of tumor grade. Knocking down TAZ inhibited glioma cell proliferation, invasion and promoted apoptosis...
October 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27759163/generation-of-a-transgenic-medaka-oryzias-latipes-strain-for-visualization-of-nuclear-dynamics-in-early-developmental-stages
#16
Takanobu Inoue, Atsuo Iida, Shingo Maegawa, Atsuko Sehara-Fujisawa, Masato Kinoshita
In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta-actin promoter. The aNLS-EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27735936/loss-of-rictor-with-aging-in-osteoblasts-promotes-age-related-bone-loss
#17
Pinling Lai, Qiancheng Song, Cheng Yang, Zhen Li, Sichi Liu, Bin Liu, Mangmang Li, Hongwen Deng, Daozhang Cai, Dadi Jin, Anling Liu, Xiaochun Bai
Osteoblast dysfunction is a major cause of age-related bone loss, but the mechanisms underlying changes in osteoblast function with aging are poorly understood. This study demonstrates that osteoblasts in aged mice exhibit markedly impaired adhesion to the bone formation surface and reduced mineralization in vivo and in vitro. Rictor, a specific component of the mechanistic target of rapamycin complex 2 (mTORC2) that controls cytoskeletal organization and cell survival, is downregulated with aging in osteoblasts...
October 13, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27714011/an-on-demand-gas-segmented-flow-generator-with-high-spatiotemporal-resolution-for-in-vivo-analysis-of-neuronal-response-in-c-elegans
#18
Liang Hu, Anle Ge, Xixian Wang, Shanshan Wang, Yue Gao, Xiaojun Feng, Wei Du, Bi-Feng Liu
Studies of chemo-sensing in C. elegans to fluctuating gaseous cues are limited due to the lack of a method of precise gas control. In this paper, we describe a microfluidic-based on-demand gas segmented flow generator for performing fluctuating gaseous stimulations to worms. This highly versatile and programmable micro-device integrated with pneumatic valves for flexible and stable gas flow control and worm immobilization enabled us to examine the temporal features of neuronal response to multiple gas pulses with sub-second precision...
October 5, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27680596/long-term-follow-up-for-anicteric-survival-with-native-liver-after-redo-kasai-a-first-report
#19
COMPARATIVE STUDY
Hiroki Nakamura, Takafumi Kawano, Katsumi Yoshizawa, Hideaki Nakajima, Kazuto Suda, Hiroyuki Koga, Kazuhiko Nakame, Satoshi Ieiri, Shigeru Takamizawa, Naoto Urushihara, Toshihiro Yanai, Atsuyuki Yamataka
PURPOSE: We present a first report of the long-term follow-up of biliary atresia (BA) patients who became anicteric with the native liver (ANL; total bilirubin <1.5mg/dL) after redo-Kasai. METHODS: Forty-six redo-Kasai cases (1984-2015) were the subjects for this study. ANL ratios were determined using the Kaplan-Meier estimate. RESULTS: BA type was I (n=3), II (n=1), and III (n=42). Mean ages (initial/redo) at Kasai were 60.3/231.9days, respectively...
December 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27591586/migration-kinetics-of-primary-aromatic-amines-from-polyamide-kitchenware-easy-and-fast-screening-procedure-using-fluorescence
#20
S Sanllorente, L A Sarabia, M C Ortiz
Primary aromatic amines, PAAs, and their derivatives constitute a health risk and control of their migration from food contact materials is the subject of permanent attention by the authorities. 25.1% of notifications made by Rapid Alert System for Food and Feed in the European Union between 2010 and 2015 concerned PAAs, polyamide cooking utensils being a common source. It is thus useful to have fast and efficient analytical methods for their control. In this work a non-separative, easy, fast and inexpensive spectrofluorimetric method based on the second order calibration of excitation-emission fluorescence matrices (EEMs) was proposed for the determination of aniline (ANL), 2,4-diaminotoluene (2,4-TDA) and 4,4'-methylenedianiline (4,4'-MDA) in polyamide cooking utensils...
November 1, 2016: Talanta
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