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https://www.readbyqxmd.com/read/27891315/awareness-about-biomedical-waste-management-and-knowledge-of-effective-recycling-of-dental-materials-among-dental-students
#1
Rajeev Ranjan, Ruchi Pathak, Dhirendra K Singh, Md Jalaluddin, Shobha A Kore, Abhijeet R Kore
AIMS AND OBJECTIVES: Biomedical waste management has become a concern with increasing number of dental practitioners in India. Being health care professionals, dentists should be aware regarding safe disposal of biomedical waste and recycling of dental materials to minimize biohazards to the environment. The aim of the present study was to assess awareness regarding biomedical waste management as well as knowledge of effective recycling and reuse of dental materials among dental students...
September 2016: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/27853455/assessment-of-the-rnasound-rna-sampling-card-for-the-preservation-of-influenza-virus-rna
#2
Hilda Lau, Aeron C Hurt
Shipping influenza virus specimens, isolates or purified RNA is normally conducted at ultra-low temperatures using dry ice to ensure minimal degradation of the samples but this is expensive and requires special packaging and shipping conditions. Therefore, alternative methods for shipping influenza viruses or RNA at ambient temperatures would be desirable. The RNASound RNA Sampling Card (FortiusBio LLC, San Diego, CA, USA) is a device that enables specimens or isolates to be applied to a card, whereby viruses are inactivated, while RNA is preserved and purified RNA can also easily be eluted...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27833227/creutzfeldt-jakob-disease-phenotype-and-course-our-experience-from-a-tertiary-center
#3
Sadanandavalli Retnaswami Chandra, Thomas Gregor Issac, Mariamma Philip, Veeranna Gadad
INTRODUCTION: Prion diseases are protein conformation disorders and neither caused by viroid or virus but is a transmissible particle labeled a prion by Pruisner. Normal prion protein becomes infectious by a different folding, but the triggers are not known. Based on the characteristic brain pathology, they are grouped under spongiform encephalopathy affecting both man and animals. Estimated prevalence is one per million. Creutzfeldt-Jakob disease (CJD) registry from National Institute and Neurosciences (NIMHANS), Bengaluru, reported 69 cases in 30 years...
September 2016: Indian Journal of Psychological Medicine
https://www.readbyqxmd.com/read/27830940/challenges-in-implementing-a-biorisk-management-program-at-universitas-indonesia-a-checklist-tool-for-biorisk-management
#4
Aroem Naroeni, Endang Winiati Bachtiar, Fera Ibrahim, Budiman Bela, Yuni Kusminanti, Ike Pujiriani, Fatma Lestari
Rapid development and advancement of bioresearch at a university's laboratories can have both positive and negative implications for public health and the environment. Many research activities in which biological materials have been created, modified, stored, and manipulated require safety procedures to keep the negative effects on humans and the environment as low as possible. The Occupational Health, Safety and Environmental (OHS&E) Department of the University of Indonesia (UI) is trying to increase the awareness and responsibility of its university members and laboratory staffs who work with biohazard materials by creating a biorisk checklist...
November 10, 2016: Health Security
https://www.readbyqxmd.com/read/27735933/the-real-time-quaking-induced-conversion-assay-for-detection-of-human-prion-disease-and-study-of-other-protein-misfolding-diseases
#5
Matthias Schmitz, Maria Cramm, Franc Llorens, Dominik Müller-Cramm, Steven Collins, Ryuichiro Atarashi, Katsuya Satoh, Christina D Orrù, Bradley R Groveman, Saima Zafar, Walter J Schulz-Schaeffer, Byron Caughey, Inga Zerr
The development and adaption of in vitro misfolded protein amplification systems has been a major innovation in the detection of abnormally folded prion protein scrapie (PrP(Sc)) in human brain and cerebrospinal fluid (CSF) samples. Herein, we describe a fast and efficient protein amplification technique, real-time quaking-induced conversion (RT-QuIC), for the detection of a PrP(Sc) seed in human brain and CSF. In contrast to other in vitro misfolded protein amplification assays-such as protein misfolding cyclic amplification (PMCA)-which are based on sonication, the RT-QuIC technique is based on prion seed-induced misfolding and aggregation of recombinant prion protein substrate, accelerated by alternating cycles of shaking and rest in fluorescence plate readers...
November 2016: Nature Protocols
https://www.readbyqxmd.com/read/27711994/methods-and-terminology-used-in-cell-culture-studies-of-low-dose-effects-of-matrix-constituents-of-polymer-resin-based-dental-materials
#6
REVIEW
Bo W Nilsen, Ulf Örtengren, Jaione Simon-Santamaria, Karen K Sørensen, Vibeke B Michelsen
General comprehension of terms and confounding factors associated with in vitro experiments can maximize the potential of in vitro testing of substances. In this systematic review, we present an overview of the terms and methods used to determine low-dose effects of matrix constituents in polymer resin-based dental materials in cell-culture studies and discuss the findings in light of how they may influence the comprehension and interpretation of results. Articles published between 1996 and 2015 were identified by searches in the Scopus, Web of Science, MEDLINE, PubMed, and Embase databases using keywords associated with low-dose effects, polymer resin-based materials, in vitro parameters, and dental materials...
December 2016: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/27583149/the-direct-environmental-impact-of-hip-arthroscopy-for-femoroacetabular-impingement-a-surgical-waste-audit-of-five-cases
#7
Darren de Sa, Kellee Stephens, Michelle Kuang, Nicole Simunovic, Jon Karlsson, Olufemi R Ayeni
Health care facilities produce significant waste (2200 kg/bed/year) creating 2% of greenhouse gas emissions and 1% total solid waste nationwide, with 20-70% of waste coming from operating rooms. We performed a waste audit of hip arthroscopy for femoroacetabular impingement (FAI) to understand its environmental impact and identify areas for greening practices. A waste audit of five hip arthroscopy procedures for FAI was performed. All waste was collected and separated into six waste streams in real time: (i) normal/landfill waste; (ii) recyclable cardboards and plastics; (iii) biohazard waste; (iv) sharp items; (v) linens and (vi) sterile wrapping...
July 2016: Journal of Hip Preservation Surgery
https://www.readbyqxmd.com/read/27470428/training-model-for-control-of-an-internal-carotid-artery-injury-during-transsphenoidal-surgery
#8
Jun Muto, Ricardo L Carrau, Kenichi Oyama, Brad A Otto, Daniel M Prevedello
OBJECTIVES: As the adoption of endoscopic endonasal approaches (EEA) continues to proliferate, increasing numbers of internal carotid artery (ICA) injuries are reported. The objective of this study was to develop a synthetic ICA injury-training model that could mimic this clinical scenario and be portable, repeatable, reproducible, and without risk of biological contamination. METHODS: Based on computed tomography of a human head, we constructed a synthetic model using selective laser sintering with polyamide nylon and glass beads...
July 28, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27420142/-occupational-injury-incidence-due-to-biological-material-in-a-tertiary-referral-public-hospital-in-santa-fe-province
#9
Romina Huanca, Luis Perretta, Natalia Lebensohn, Oscar Di Paolo, Miryam Pires, Nora Beatriz Quaglia
UNLABELLED: Occupational accidents due to biological materials cause significant harm to workers and institutions. OBJECTIVE: To estimate the risk of accidents in doctors and nurses at the Hospital Provincial del Centenario (HPC) and particularly in Emergency Medical Service (EMS) between 2009 and 2013. METHODS: retrospective cohort study from the survey information of injuries by accidents with biohazard in doctors and nurses workers from HPC...
2016: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/27385074/gaseous-and-particulate-content-of-laser-hair-removal-plume
#10
Gary S Chuang, William Farinelli, David C Christiani, Robert F Herrick, Norman C Y Lee, Mathew M Avram
Importance: Potentially harmful chemicals are released when tissues are vaporized. Laser hair removal (LHR) causes heating and often vaporization of hairs, producing both a signature malodorous plume and visible particulates. Objective: To characterize the chemical composition and quantify the ultrafine particle content of the plume generated during LHR. Design, Setting, and Participants: In the laser center of a large academic hospital, discarded terminal hairs from the trunk and extremities were collected from 2 adult volunteers...
July 6, 2016: JAMA Dermatology
https://www.readbyqxmd.com/read/27377266/evaluation-of-diagnostic-performance-of-rk28-elisa-using-urine-for-diagnosis-of-visceral-leishmaniasis
#11
Prakash Ghosh, Khondaker R H Bhaskar, Faria Hossain, Md Anik Ashfaq Khan, Aarthy C Vallur, Malcolm S Duthie, Shinjiro Hamano, Md Abdus Salam, M Mamun Huda, Md Gulam Musawwir Khan, Rhea N Coler, Steven G Reed, Dinesh Mondal
BACKGROUND: Recombinant fusion proteins are now commonly used to detect circulating antibodies for the serodiagnosis of visceral leishmaniasis (VL) in Asia, Africa and the Americas. Although simple, these tests still require blood collection and their use in remote settings can be limited due to the need of collection devices, serum fractionation instrument and generation of biohazardous waste. The development of an accurate and non-invasive diagnostic algorithm for VL, such as could be achieved with urine, is desirable...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27314556/enhanced-simultaneous-pec-eradication-of-bacteria-and-antibiotics-by-facilely-fabricated-high-activity-001-facets-tio2-mounted-onto-tio2-nanotubular-photoanode
#12
Guiying Li, Xin Nie, Jiangyao Chen, Po Keung Wong, Taicheng An, Hiromi Yamashita, Huijun Zhao
Biohazards and coexisted antibiotics are two groups of emerging contaminants presented in various aquatic environments. They can pose serious threat to the ecosystem and human health. As a result, inactivation of biohazards, degradation of antibiotics, and simultaneous removal of them are highly desired. In this work, a novel photoanode with a hierarchical structured {001} facets exposed nano-size single crystals (NSC) TiO2 top layer and a perpendicularly aligned TiO2 nanotube array (NTA) bottom layer (NSC/NTA) was successfully fabricated...
September 15, 2016: Water Research
https://www.readbyqxmd.com/read/27265152/rapidly-separating-microneedles-for-transdermal-drug-delivery
#13
Dan Dan Zhu, Qi Lei Wang, Xu Bo Liu, Xin Dong Guo
UNLABELLED: The applications of polymer microneedles (MNs) into human skin emerged as an alternative of the conventional hypodermic needles. However, dissolving MNs require many minutes to be dissolved in the skin and typically have difficulty being fully inserted into the skin, which may lead to the low drug delivery efficiency. To address these issues, we introduce rapidly separating MNs that can rapidly deliver drugs into the skin in a minimally invasive way. For the rapidly separating MNs, drug loaded dissolving MNs are mounted on the top of solid MNs, which are made of biodegradable polylactic acid which eliminate the biohazardous waste...
September 1, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27122199/evaluating-3d-printing-to-solve-the-sample-to-device-interface-for-lrs-and-poc-diagnostics-example-of-an-interlock-meter-mix-device-for-metering-and-lysing-clinical-urine-samples
#14
Erik Jue, Nathan G Schoepp, Daan Witters, Rustem F Ismagilov
This paper evaluates the potential of 3D printing, a semi-automated additive prototyping technology, as a means to design and prototype a sample-to-device interface, amenable to diagnostics in limited-resource settings, where speed, accuracy and user-friendly design are critical components. As a test case, we built and validated an interlock meter-mix device for accurately metering and lysing human urine samples for use in downstream nucleic acid amplification. Two plungers and a multivalve generated and controlled fluid flow through the device and demonstrate the utility of 3D printing to create leak-free seals...
May 21, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27077002/genetic-diversity-in-oryza-glumaepatula-wild-rice-populations-in-costa-rica-and-possible-gene-flow-from-o-sativa
#15
Eric J Fuchs, Allan Meneses Martínez, Amanda Calvo, Melania Muñoz, Griselda Arrieta-Espinoza
Wild crop relatives are an important source of genetic diversity for crop improvement. Diversity estimates are generally lacking for many wild crop relatives. The objective of the present study was to analyze how genetic diversity is distributed within and among populations of the wild rice species Oryza glumaepatula in Costa Rica. We also evaluated the likelihood of gene flow between wild and commercial rice species because the latter is commonly sympatric with wild rice populations. Introgression may change wild species by incorporating alleles from domesticated species, increasing the risk of losing original variation...
2016: PeerJ
https://www.readbyqxmd.com/read/27019541/pathogenic-yet-environmentally-friendly-black-fungal-candidates-for-bioremediation-of-pollutants
#16
Barbara Blasi, Caroline Poyntner, Tamara Rudavsky, Francesc X Prenafeta-Boldú, Sybren De Hoog, Hakim Tafer, Katja Sterflinger
A collection of 163 strains of black yeast-like fungi from the CBS Fungal Biodiversity Center (Utrecht, The Netherlands), has been screened for the ability to grow on hexadecane, toluene and polychlorinated biphenyl 126 (PCB126) as the sole carbon and energy source. These compounds were chosen as representatives of relevant environmental pollutants. A microtiter plate-based culture assay was set up in order to screen the fungal strains for growth on the selected xenobiotics versus glucose, as a positive control...
March 15, 2016: Geomicrobiology Journal
https://www.readbyqxmd.com/read/27000034/feasibility-acceptability-and-impact-of-integrating-malaria-rapid-diagnostic-tests-and-pre-referral-rectal-artesunate-into-the-integrated-community-case-management-programme-a-pilot-study-in-mchinji-district-malawi
#17
Themba B Phiri, Blessings N Kaunda-Khangamwa, Andrew Bauleni, Tiyese Chimuna, David Melody, Humphreys Kalengamaliro, John H Sande, Humphreys Kampira Nsona, Don P Mathanga
BACKGROUND: The World Health Organization recommends that persons of all ages suspected of malaria should receive a parasitological confirmation of malaria by use of malaria rapid diagnostic test (RDT) at community level, and that rectal artesunate should be used as a pre-referral treatment for severe malaria to rapidly reduce parasitaemia. This paper reports on findings from a pilot study that assessed the feasibility, acceptability and effects of integrating RDTs and pre-referral rectal artesunate into the integrated Community Case Management programme in Malawi...
2016: Malaria Journal
https://www.readbyqxmd.com/read/26980170/hazardous-drugs-new-challenges-new-opportunities
#18
Silvia Valero García, Eduardo López Briz, Nieves Vila Clérigues, Jose Luis Poveda Andrés
Occupational exposure to hazardous drugs can cause harmful effects on health professionals and several protective measures must be taken. Nevertheless, classification of hazardous drugs is not the same in all the published repertoires and the terminology is still confusing: hazardous drugs, biohazardous drugs or risky drugs are terms improperly described and can define very different drugs with a very different hazard profiles. In Spain, there is not an updated official list of hazardous drugs, and healthcare professionals must consider and follow other published lists...
March 2016: Farmacia Hospitalaria
https://www.readbyqxmd.com/read/26978389/characterization-of-viral-communities-of-biting-midges-and-identification-of-novel-thogotovirus-species-and-rhabdovirus-genus
#19
Sarah Temmam, Sonia Monteil-Bouchard, Catherine Robert, Jean-Pierre Baudoin, Masse Sambou, Maxence Aubadie-Ladrix, Noémie Labas, Didier Raoult, Oleg Mediannikov, Christelle Desnues
More than two thirds of emerging viruses are of zoonotic origin, and among them RNA viruses represent the majority. Ceratopogonidae (genus Culicoides) are well-known vectors of several viruses responsible for epizooties (bluetongue, epizootic haemorrhagic disease, etc.). They are also vectors of the only known virus infecting humans: the Oropouche virus. Female midges usually feed on a variety of hosts, leading to possible transmission of emerging viruses from animals to humans. In this context, we report here the analysis of RNA viral communities of Senegalese biting midges using next-generation sequencing techniques as a preliminary step toward the identification of potential viral biohazards...
March 2016: Viruses
https://www.readbyqxmd.com/read/26785769/pilot-study-of-a-rapid-and-minimally-instrumented-sputum-sample-preparation-method-for-molecular-diagnosis-of-tuberculosis
#20
Tanya M Ferguson, Kris M Weigel, Annie Lakey Becker, Delia Ontengco, Masahiro Narita, Ilya Tolstorukov, Robert Doebler, Gerard A Cangelosi, Angelika Niemz
Nucleic acid amplification testing (NAAT) enables rapid and sensitive diagnosis of tuberculosis (TB), which facilitates treatment and mitigates transmission. Nucleic acid extraction from sputum constitutes the greatest technical challenge in TB NAAT for near-patient settings. This report presents preliminary data for a semi-automated sample processing method, wherein sputum is disinfected and liquefied, followed by PureLyse(®) mechanical lysis and solid-phase nucleic acid extraction in a miniaturized, battery-operated bead blender...
2016: Scientific Reports
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