keyword
MENU ▼
Read by QxMD icon Read
search

hybridization chain reaction

keyword
https://www.readbyqxmd.com/read/29353431/sensitive-electrochemical-detection-of-microrna-21-based-on-propylamine-functionalized-mesoporous-silica-with-glucometer-readout
#1
Kun Deng, Yong Zhang, Xuedong Tong
A new homogeneous electrochemical sensing system was developed for sensitive detection of microRNA-21 (miRNA-21) based on target-induced glucose release from propylamine-functionalized mesoporous silica nanoparticle (MSN) with glucometer readout. Glucose molecules (as the signal tracers) were initially gated into the pores through the interaction of the negatively charged anchor DNA with the aminated MSN. Upon addition of target miRNA, the analyte competitively hybridized with anchor DNA to form the RNA-DNA duplex, thus resulting in detachment of anchor DNA from the MSN accompanying the pore opening...
January 20, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29350910/highly-asymmetric-phase-behaviors-of-polyhedral-oligomeric-silsesquioxanes-based-multi-headed-giant-surfactants
#2
Mingjun Huang, Kan Yue, Jiahao Huang, Chang Liu, Zhe Zhou, Jing Wang, Kan Wu, Wenpeng Shan, An-Chang Shi, Stephen Z D Cheng
This work reports the molecular design, synthesis, and systematic study on the bulk self-assembly behaviors of three series of polyhedral oligomeric silsesquioxanes (POSS)-based multi-headed giant surfactants XDPOSS-PSn (X = 2, 3 and 4), which are composed of two, three or four hydrophilic hydroxyl-group functionalized DPOSS cages attached via one junction point to a hydrophobic polystyrene (PS) chain. These series of hybrid polymeric amphiphiles with precisely defined chemical structure and controllable molecular architecture are synthesized by the sequential usage of "click" reactions...
January 19, 2018: ACS Nano
https://www.readbyqxmd.com/read/29346450/cloning-and-spatiotemporal-expression-of-xenopus-laevis-apolipoprotein-ci
#3
Jyotsna Sridharan, Tomomi Haremaki, Daniel C Weinstein
Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer's disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest...
2018: PloS One
https://www.readbyqxmd.com/read/29344131/a-novel-variant-translocation-1-9-p22-q34-resulting-in-a-dek-nup214-fusion-gene-in-a-patient-with-acute-myeloid-leukemia-a-case-report
#4
Qishan Hao, Qi Zhang, Chengwen Li, Shuning Wei, Qinghua Li, Yang Song, Yingchang Mi
The present case report describes a 46-year-old female patient diagnosed with M4 acute myeloid leukemia (AML), accompanied with a t(1;9)(p22;q34) chromosomal abnormality. Transcriptome sequencing identified a DEK proto-oncogene (DEK)/nucleoporin (NUP)214 fusion gene, which results from the t(6;9)(p23;q34) chromosomal translocation. Polymerase chain reaction analysis and fluorescence in situ hybridization were used to verify the existence of the DEK/NUP214 fusion gene. Few patients with AML with the t(6;9)(p23;q34) chromosomal translocation have been reported to have other chromosomal or karyotype changes...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343979/the-influence-of-cyp3a5-polymorphisms-on-haloperidol-treatment-in-patients-with-alcohol-addiction
#5
Mikhail Sergeevich Zastrozhin, Elena Anatolievna Grishina, Kristina Anatolievna Ryzhikova, Valery Valerievich Smirnov, Ludmila Mikhailovna Savchenko, Evgeny Alekseevich Bryun, Dmitry Alekseevich Sychev
Background: Isoenzymes CYP2D6 and CYP3A4, the activity of which varies widely, are involved in metabolism of haloperidol and may influence its profile of efficacy and safety. Objective: The primary aim of this study was to estimate the relationship between CYP3A5 gene polymorphism, activity of the CYP3A isoenzyme, and the risk of development of adverse drug reactions by haloperidol in patients with alcohol abuse. Methods: Sixty-six male alcohol-addicted patients participated in the study...
2018: Pharmacogenomics and Personalized Medicine
https://www.readbyqxmd.com/read/29343880/novel-application-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-gene-translocation
#6
Ibu Matsuzaki, Hideto Iguchi, Yurina Mikasa, Hiromu Morishita, Katsuya Okuda, Keita Nakaguchi, Yuki Mori, Yoshifumi Iwahashi, Kenji Warigaya, Masakazu Fujimoto, Fumiyoshi Kojima, Shin-Ichi Murata
Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP)...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343198/localization-of-felis-catus-papillomavirus-type-2-e6-and-e7-rna-in-feline-cutaneous-squamous-cell-carcinoma
#7
Nathan Hoggard, John S Munday, Jennifer Luff
Findings from polymerase chain reaction-based methods have suggested a role of Felis catus papillomavirus 2 (FcaPV-2) in the development of feline cutaneous squamous cell carcinoma (SCC). However, because polymerase chain reaction cannot localize deoxyribonucleic acid or ribonucleic acid within the lesion, it is difficult to differentiate a coincidental FcaPV-2 infection and a causative association. Given that a key event in the pathogenesis of human papillomavirus-induced cancer is the expression of viral E6 and E7 oncogenes, localization of FcaPV-2 E6 and E7 transcription within neoplastic cells in feline SCCs would support a causative role for this papillomavirus...
January 1, 2018: Veterinary Pathology
https://www.readbyqxmd.com/read/29340846/novel-isoforms-of-n16-and-n19-families-implicated-for-the-nacreous-layer-formation-in-the-pearl-oyster-pinctada-fucata
#8
Fumito Ohmori, Shigeharu Kinoshita, Daisuke Funabara, Hiroki Koyama, Kiyohito Nagai, Kaoru Maeyama, Kikuhiko Okamoto, Shuichi Asakawa, Shugo Watabe
Although a wide variety of proteins and genes possibly related to the shell formation in bivalve have been identified, their functions have been only partially approved. We have recently performed deep sequencing of expressed sequence tags (ESTs) from the pearl oyster Pinctada fucata using a next-generation sequencer, identifying a dozen of novel gene candidates which are possibly associated with the nacreous layer formation. Among the ESTs, we focused on three novel isoforms (N16-6, N16-7, and N19-2) of N16 and N19 families with reference to five known genes in the families and determined the full-length cDNA sequences of these isoforms...
January 16, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29340411/multiplexed-detection-of-nucleic-acids-using-19f-nmr-chemical-shift-changes-based-on-dna-photo-cross-linking-of-3-vinylcarbazole-derivatives
#9
Shigetaka Nakamura, Yasuharu Takashima, Kenzo Fujimoto
The detection methodology for nucleic acids is a useful tool for the analysis of biological systems and diagnosis of diseases. We demonstrated the feasibility of the detection of any nucleic acids based on large chemical shifts via ultrafast DNA photo-cross-linking and the effects of substitution by 3-vinylcarbazole derivatives. These chemical shifts enable the sequence-specific detection of any strand using hybridization chain reaction.
January 17, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29335378/comparison-of-telomere-length-measurement-methods
#10
REVIEW
Tsung-Po Lai, Woodring E Wright, Jerry W Shay
The strengths and limitations of the major methods developed to measure telomere lengths (TLs) in cells and tissues are presented in this review. These include Q-PCR (Quantitative Polymerase Chain Reaction), TRF (Terminal Restriction Fragment) analysis, a variety of Q-FISH (Quantitative Fluorescence In Situ Hybridization) methods, STELA (Single TElomere Length Analysis) and TeSLA (Telomere Shortest Length Assay). For each method, we will cover information about validation studies, including reproducibility in independent laboratories, accuracy, reliability and sensitivity for measuring not only the average but also the shortest telomeres...
March 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29334386/organic-inorganic-hybrids-having-a-talc-like-structure-as-suitable-hosts-to-guest-a-wide-range-of-species
#11
J Brendlé
The sol-gel process involving hydrolysis and condensation reactions is an attractive way to form siloxane based hybrid materials since it is a one-step method performed under mild conditions. Organic-inorganic hybrids having a talc-like structure (TLH) can be obtained using this procedure starting from organotrialkoxysilanes having the formula R-Si(OR')3 (where R stands for an organic moiety and R' for a methyl or ethyl group) with different functionalities, an aqueous or ethanolic solution of a magnesium salt, and in some cases, an aqueous solution of sodium hydroxide...
January 15, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29329703/highly-sensitive-microrna-detection-based-on-bio-bar-code-assay-and-catalytic-hairpin-assembly-two-stage-amplification
#12
Songsong Tang, Yuan Gu, Huiting Lu, Haifeng Dong, Kai Zhang, Wenhao Dai, Xiangdan Meng, Fan Yang, Xueji Zhang
Herein, a highly-sensitive microRNA (miRNA) detection strategy was developed by combining bio-bar-code assay (BBA) with catalytic hairpin assembly (CHA). In the proposed system, two nanoprobes of magnetic nanoparticles functionalized with DNA probes (MNPs-DNA) and gold nanoparticles with numerous barcode DNA (AuNPs-DNA) were designed. In the presence of target miRNA, the MNP-DNA and AuNP-DNA hybridized with target miRNA to form a "sandwich" structure. After "sandwich" structures were separated from the solution by the magnetic field and dehybridized by high temperature, the barcode DNA sequences were released by dissolving AuNPs...
April 3, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29329073/nanobiostructure-of-fibrous-like-alumina-functionalized-with-an-analog-of-the-bp100-peptide-synthesis-characterization-and-biological-applications
#13
L M F C Torres, N A Braga, I P Gomes, M T Almeida, T L Santos, J P de Mesquita, L M da Silva, H R Martins, K C Kato, W T P Dos Santos, J M Resende, M C Pereira, M P Bemquerer, M A Rodrigues, R M Verly
The functionalization of alumina nanoparticles of specific morphology with antimicrobial peptides (AMP) can be a promising strategy for modeling medical devices and packaging materials for cosmetics, medicines or food, since the contamination by pathogens could be reduced. In this paper, we show the synthesis of a fibrous-like alumina nanobiostructure, as well as its functionalization with the peptide EAAA-BP100, an analog of the antimicrobial peptide BP100. The antibacterial activity of the obtained material against some bacterial strains is also investigated...
January 3, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29311262/multidimensional-quantitative-analysis-of-mrna-expression-within-intact-vertebrate-embryos
#14
Vikas Trivedi, Harry M T Choi, Scott E Fraser, Niles A Pierce
For decades, in situ hybridization methods have been essential tools for studies of vertebrate development and disease, as they enable qualitative analyses of mRNA expression in an anatomical context. Quantitative mRNA analyses typically sacrifice the anatomy, relying on embryo microdissection, dissociation, cell sorting and/or homogenization. Here, we eliminate the trade-off between quantitation and anatomical context, using quantitative in situ hybridization chain reaction (qHCR) to perform accurate and precise relative quantitation of mRNA expression with subcellular resolution within whole-mount vertebrate embryos...
January 8, 2018: Development
https://www.readbyqxmd.com/read/29310580/evaluation-of-fluorescent-in-situ-hybridization-technique-for-diagnosis-of-malaria-in-ahero-sub-county-hospital-kenya
#15
Regina Kandie, Rachel Ochola, Kariuki Njaanake
BACKGROUND: Malaria is a major cause of morbidity and mortality. Treatment of malaria in a timely manner could avert deaths. Treatment ultimately relies on the rapid and accurate diagnosis. Fluorescence in situ hybridization (FISH), a cytogenetic technique based on detection of specific nucleic acid, has the potential to address the limitations of the current diagnostic approaches. This study investigates further the performance of FISH for the diagnosis of malaria in a rural setting in Western Kenya...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29310253/an-electrochemical-aptasensor-for-the-highly-sensitive-detection-of-8-hydroxy-2-deoxyguanosine-based-on-the-hybridization-chain-reaction
#16
Li-Ping Jia, Li-Juan Wang, Rong-Na Ma, Lei Shang, Wei Zhang, Qing-Wang Xue, Huai-Sheng Wang
In the present work a highly sensitive and selective aptasensor was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) based on the hybridization chain reaction (HCR) signal amplification. It was observed that the aptamer of 8-OH-dG could hybridize with the capture DNA immobilized on the gold electrode with a sticky tail left, which initiated the HCR and led to the formation of extended dsDNA structure on the electrode surface. Then the electroactive species ([Ru(NH3)6]3+, RuHex) intercalated into the dsDNA grooves to generate the amplified signal...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29306192/comparative-study-of-tribochemistry-of-ultrahigh-molecular-weight-polyethylene-polyphenylene-sulfide-and-polyetherimide-in-tribo-composites
#17
Huimin Qi, Ligang Zhang, Ga Zhang, Tingmei Wang, Qihua Wang
HYPOTHESIS: Tribochemical reactions of polymer matrix with steel counterpart can exert an important role in tribofilm's structure, and thereby affect the tribological performance of its composites. EXPERIMENTS: In the present work, tribochemistry of ultrahigh molecular weight polyethylene (UHMWPE), polyphenylene sulfide (PPS) and polyetherimide (PEI) in tribo-composites was comparatively studied. Two kinds of formulations, i.e. conventional composites filled with carbon fibers and graphite and hybrid nanocomposites containing additional silica (SiO2) nanoparticles, were investigated...
December 29, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29306030/exonuclease-iii-boosted-cascade-reactions-for-ultrasensitive-sers-detection-of-nucleic-acids
#18
Yudie Sun, Pai Peng, Ruiyan Guo, Huihui Wang, Tao Li
A variety of nucleic acid amplification techniques have been integrated into different detection methods to promote the development of sensitive and convenient analysis of nucleic acids. However, it is still in urgent need to develop amplified nucleic acid biosensors for the analysis of susceptible gene and even distinguishing single-base mismatched DNA in complex biological samples. Benefiting from the achieved detection strategies, here we boost isothermal nucleic acid amplification by resorting to enzyme amplification, and combine this two-stage amplification method with surface-enhanced Raman spectroscopy (SERS) to develop a signal-on nucleic acid detection platform...
December 29, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29305975/identification-and-expression-analysis-of-micrornas-in-medaka-gonads
#19
Weiwei Qiu, Yefei Zhu, Yun Wu, Cancan Yuan, Kerang Chen, Mingyou Li
Gonad development is a highly regulated, coordinated biological process and increasing evidences have indicated that microRNA (miRNA) may be involved in this dynamic program. Medaka (Oryzias latipes) is a good model for reproductive research as it has distinct sex determining genes, however, research in gonadal miRNAs is lacked. In this study, two small RNA libraries from the ovaries and testes were constructed and sequenced. A total of 285 conserved and 388 novel miRNAs were obtained, among which, 142 mature miRNAs were significantly (> two-fold change) up or down regulated in the testis compared to the ovary...
January 3, 2018: Gene
https://www.readbyqxmd.com/read/29304903/locked-nucleic-acid-technology-for-highly-sensitive-detection-of-somatic-mutations-in-cancer
#20
Takayuki Ishige, Sakae Itoga, Kazuyuki Matsushita
The molecular diagnosis of the cancer mutational status is essential for modern clinical laboratory medicine. Mutations in EGFR, KRAS, BRAF, and PIK3CA genes are widely analyzed in solid tumors such as lung cancer, colorectal cancer, breast cancer, and melanoma. The allele-specific polymerase chain reaction, high-resolution melting, and Sanger sequencing are used for detecting and identifying gene mutations in many clinical laboratories. The locked nucleic acid (LNA) is a class of nucleic acid analogs that contain a methylene bridge connecting the 2' oxygen and 4' carbon in the ribose moiety...
2018: Advances in Clinical Chemistry
keyword
keyword
92022
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"