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https://www.readbyqxmd.com/read/28544921/in-situ-formed-copper-nanoparticles-templated-by-tdt-mediated-dna-for-enhanced-spr-sensor-based-dna-assay
#1
Pei-Xin Yuan, Sheng-Yuan Deng, Chen-Yu Zheng, Serge Cosnier, Dan Shan
For the efficient surface plasmon resonance (SPR)-based DNA assay researching, signal amplification tactics were absolutely necessary. In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT)-mediated extension, and synergistically with nano-effect deposition as the mass relay. The objective of this strategy was manifold: firstly, tDNA hybridized with the optimal designed probes to active the TdT-mediated DNA extension onto the surface of SPR chip, resulted a long poly-T sequences ssDNA chain in dsDNA terminal onto surface of gold chip and characterized by SPR signal amplitudes...
May 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28544318/neurologic-clinical-signs-in-cattle-with-astrovirus-associated-encephalitis
#2
R Deiss, S Selimovic-Hamza, T Seuberlich, M Meylan
BACKGROUND: Evidence of neurotropic astroviruses has been established using novel genetic methods in cattle suffering from viral encephalitis of previously unknown origin. OBJECTIVES: To describe the clinical signs observed in cattle with astrovirus-associated encephalitis. ANIMALS: Eight cattle (4 cows, 3 heifers, and 1 bull of 4 different breeds) admitted to the Clinic for Ruminants for neurologic disease and 1 cow investigated in the field...
May 22, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28536710/a-label-free-ratiometric-electrochemical-dna-sensor-for-monitoring-intracellular-redox-homeostasis
#3
Xinping Liu, Zhengqing Yan, Yuhuan Sun, Jinsong Ren, Xiaogang Qu
A label-free ratiometric electrochemical biosensor was constructed for monitoring intracellular redox homeostasis by DNA metallization-mediated hybridization chain reaction amplification. This platform could also be used to discriminate cancer cells and normal cells.
May 24, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28531156/molecular-techniques-for-the-detection-of-organisms-in-aquatic-environments-with-emphasis-on-harmful-algal-bloom-species
#4
REVIEW
Linda K Medlin, Jahir Orozco
Molecular techniques to detect organisms in aquatic ecosystems are being gradually considered as an attractive alternative to standard laboratory methods. They offer faster and more accurate means of detecting and monitoring species, with respect to their traditional homologues based on culture and microscopic counting. Molecular techniques are particularly attractive when multiple species need to be detected and/or are in very low abundance. This paper reviews molecular techniques based on whole cells, such as microscope-based enumeration and Fluorescence In-Situ Hybridization (FISH) and molecular cell-free formats, such as sandwich hybridization assay (SHA), biosensors, microarrays, quantitative polymerase chain reaction (qPCR) and real time PCR (RT-PCR)...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28527622/genome-wide-copy-number-analysis-reveals-candidate-gene-loci-that-confer-susceptibility-to-high-grade-prostate-cancer
#5
Prevathe Poniah, Shamsul Mohd Zain, Azad Hassan Abdul Razack, Shanggar Kuppusamy, Shankar Karuppayah, Hooi Sian Eng, Zahurin Mohamed
BACKGROUND: Two key issues in prostate cancer (PCa) that demand attention currently are the need for a more precise and minimally invasive screening test owing to the inaccuracy of prostate-specific antigen and differential diagnosis to distinguish advanced vs. indolent cancers. This continues to pose a tremendous challenge in diagnosis and prognosis of PCa and could potentially lead to overdiagnosis and overtreatment complications. Copy number variations (CNVs) in the human genome have been linked to various carcinomas including PCa...
May 17, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28527402/a-rare-e13a3-b2a3-bcr-abl1-fusion-transcript-with-normal-karyotype-in-chronic-myeloid-leukemia-the-challenges-in-diagnosis-and-monitoring-minimal-residual-disease-mrd
#6
M-H Duan, H Li, H Cai
Patients with chronic myeloid leukemia (CML) have a t (9;22)(q34;q11.2) or variant translocation that results in a BCR-ABL1 fusion gene. For many years, conventional karyotyping has been used as the standard diagnostic tool for t (9;22) (q34;q11.2). However, it has several limitations that may lead to failure for detecting BCR-ABL1 gene rearrangements in around 5% of all CML patients. Although reverse transcription polymerase chain reaction (RT-PCR) has evolved as a sensitive method for detecting BCR-ABL1 translocation, this method fail to detect certain BCR-ABL1 fusion transcript type, such as e13a3 (also known as b2a3), as a result of many commercially available and laboratory-developed primer sets...
May 12, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28522294/activating-transcription-factor-3-promotes-spinal-cord-regeneration-of-adult-zebrafish
#7
Lin-Fang Wang, Shu-Bing Huang, Hou-De Zhao, Chun-Jie Liu, Li Yao, Yan-Qin Shen
Zebrafish is an excellent model to study the mechanisms underlying successful central nervous system (CNS) regeneration. Previous study shows that activating transcription factor 3 (ATF3) promotes neurite outgrowth and is involved in optic nerve regeneration in zebrafish. Here, we used zebrafish model to investigate the role of ATF3 in regeneration following spinal cord injury (SCI). Quantitative polymerase chain reaction (qPCR) and in situ hybridization revealed that ATF3 mRNA levels increased at 12 h and 6 d following SCI...
May 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28521575/high-frequency-of-de-novo-daz-microdeletion-in-sperm-nuclei-of-subfertile-men-possible-involvement-of-genome-instability-in-idiopathic-male-infertility
#8
Hossein Mozdarani, Pegah Ghoraeian, Sohail Mozdarani, Parvin Fallahi, Anahita Mohseni-Meybodi
The occurrence and diagnosis of Y-chromosome microdeletions, specifically deletions of the DAZ (Deleted in Azoospermia) genes are an important issue in male infertility. Screening Y chromosome microdeletion is mainly done using polymerase chain reaction (PCR) on blood leukocytes. However, there is some evidence indicating that presence of DAZ in somatic cells might not be indicative of its presence in the germ cell lineage. Therefore, a total of 130 men with poor semen quality were examined for presence of DAZ microdeletion in their leukocytes...
May 19, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28514850/dna-dendrimer-streptavidin-nanocomplex-an-efficient-signal-amplifier-for-construction-of-biosensing-platforms
#9
Yan Zhao, Shichao Hu, Huaming Wang, Kaiwen Yu, Yan Guan, Xiaoyun Liu, Na Li, Feng Liu
We develop a DNA dendrimer-streptavidin (SA) nanocomplex as a novel signal amplifier to create biosensing platforms for disease-related species. The DNA dendrimer-SA nanocomplex is fabricated by cross-linking the nonlinear hybridization chain reaction based DNA dendrimer with the SA-coupled linker DNA and possesses multiple sticky ends, a high molecular weight, and a hyperbranched nanostructure with large numbers of DNA duplexes. Taking advantage of the DNA dendrimer-SA nanocomplex and a label-free quartz crystal microbalance (QCM) technology, we first construct a mass-sensitive QCM biosensing platform for nucleic acids, which displays high selectivity and sensitivity, with a detection limit of 0...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28514168/regioselective-carbyne-transfer-to-ring-opening-alkyne-metathesis-initiators-gives-access-to-telechelic-polymers
#10
Stephen von Kugelgen, Renee Sifri, Donatela Bellone, Felix R Fischer
Regioselective carbyne-transfer reagents derived from (3,3,3-trifluoroprop-1-yn-1-yl)benzene give access to functionalized ring-opening alkyne metathesis polymerization (ROAMP) initiators [R-C6H4C≡Mo(OC(CH3)(CF3)2)3] featuring electron-donating or -withdrawing substituents on the benzylidyne. Kinetic studies and linear free-energy relationships reveal that the initiation step of the ring-opening alkyne metathesis polymerization of 5,6,11,12-tetradehydrobenzo[a,e][8]annulene exhibits a moderate positive Hammett reaction constant (ρ = +0...
May 24, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28513652/an-electrochemiluminescence-assay-for-sensitive-detection-of-methyltransferase-activity-in-different-cancer-cells-by-hybridization-chain-reaction-coupled-with-a-g-quadruplex-hemin-dnazyme-biosensing-strategy
#11
Hui Zhang, Zhihui Guo, Huilei Dong, Hongfei Chen, Chenxin Cai
In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (ΔI) allowed detection of DNMT1 activity down to 0.09 U mL(-1), and ΔI was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL(-1) in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells...
May 17, 2017: Analyst
https://www.readbyqxmd.com/read/28509589/characteristics-and-expression-profile-of-krt71-screened-by-suppression-subtractive-hybridization-cdna-library-in-curly-fleece-chinese-tan-sheep
#12
Xiaolong Kang, Yufang Liu, Jibin Zhang, Qinqin Xu, Chengkun Liu, Meiying Fang
As an important commercial trait for sheep, curly fleece has a great economic impact on production costs and efficiency in sheep industry. To identify genes that are important for curly fleece formation in mammals, a suppression subtractive hybridization analysis was performed on the shoulder skin tissues exposed to two different growth stages of Chinese Tan sheep with different phenotypes (curly fleece and noncurling fleece). BLAST analysis identified 67 differentially expressed genes, of which 31 were expressed lower and 36 were expressed higher in lambs than in adult sheep...
May 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28507706/ph-and-ligand-induced-release-of-loads-from-dna-acrylamide-hydrogel-microcapsules
#13
Wei-Ching Liao, Sivan Lilienthal, Jason S Kahn, Marianna Riutin, Yang Sung Sohn, Rachel Nechushtai, Itamar Willner
Herein, a method to construct stimuli-responsive DNA-acrylamide-based hydrogel microcapsules has been presented. This method involves the use of polyacrylamide chains modified with predesigned nucleic acid hairpin units and optionally single-strand tethers that provide the required hybridization and recognition functions to yield substrate-loaded stimuli-responsive hydrogel-based microcapsules. The synthesis of the microcapsules involves the loading of CaCO3 microparticles with the respective load substrates and the functionalization of the CaCO3 template particles with nucleic acid promoter units...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28505492/comprehensive-evaluation-of-scfa-production-in-the-intestinal-bacteria-regulated-by-berberine-using-gas-chromatography-combined-with-polymerase-chain-reaction
#14
Lu-Lu Wang, Hui-Hui Guo, Shuai Huang, Chen-Lin Feng, Yan-Xing Han, Jian-Dong Jiang
Short-chain fatty acids (SCFAs) of intestine microbial have caught accumulating attention for their beneficial effects on human health. Botanic compounds with low bioavailability such as berberine (BBR) and resveratrol might interact with intestinal microbial ecosystem and promote gut bacteria to produce SCFA, which contribute to their biological effects. In the present study, a comprehensive assay system was built to detect SCFAs production in intestinal bacteria, in which stringent anaerobic culture was applied for in vitro bacterial fermentation, followed by direct-injection GC detection (chemical detection) in combination with real time polymerase chain reaction (RT-PCR, biological detection)...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28502712/ligation-mediated-pcr-with-a-back-to-back-adapter-reduces-amplification-bias-resulting-from-variations-in-gc-content
#15
Satoru Ishihara, Naoe Kotomura, Naoki Yamamoto, Hiroshi Ochiai
Ligation-mediated polymerase chain reaction (LM-PCR) is a common technique for amplification of a pool of DNA fragments. Here, a double-stranded oligonucleotide consisting of two primer sequences in back-to-back orientation was designed as an adapter for LM-PCR. When DNA fragments were ligated with this adapter, the fragments were sandwiched between two adapters in random orientations. In the ensuing PCR, ligation products linked at each end to an opposite side of the adapter, i.e. to a distinct primer sequence, were preferentially amplified compared with products linked at each end to an identical primer sequence...
May 11, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28501627/detection-of-viromes-of-rna-viruses-using-the-next-generation-sequencing-libraries-prepared-by-three-methods
#16
Yuan Qiu, Ji-Ming Chen, Tong Wang, Guang-Yu Hou, Qing-Ye Zhuang, Run Wu, Kai-Cheng Wang
Virome (viral megagenomics) detection using next generation sequencing has been widely applied in virology, but its methods remain complicated and need optimization. In this study, we detected the viromes of RNA viruses of one mock sample, one pooled duck feces sample and one pooled mink feces sample on the Personal Genome Machine platform using the sequencing libraries prepared by three methods. The sequencing primers were added through random hybridization and ligation to fragmented viral RNA using a RNA-Seq kit in method 1, through random reverse transcription (RT) and polymerase chain reaction (PCR) in method 2 which was developed in our laboratory, and through hybridization and ligation to fragmented amplicons of random RT-PCR using a single primer in method 3...
May 10, 2017: Virus Research
https://www.readbyqxmd.com/read/28501216/intracellular-dna-and-microrna-sensing-based-on-metal-organic-framework-nanosheets-with-enzyme-free-signal-amplification
#17
Wen-Jun Song
A new fluorescent sensing platform based on ultrathin metal-organic framework (MOF) nanosheets (MnDMS) was prepared from the flexible ligand 2,2-dimethylsuccinate and Mn ions. The MnDMS nanoparticles can be obtained by simply ultrasonication of the MnDMS crystal, and then can be exfoliated into nanosheets by Li-intercalation method. The MnDMS nanosheets can be easily assembled with biological probes, leading to efficient fluorescence quenching of the fluorophore tagged ssDNA and microRNA (miRNA). By using a hybridization chain reaction (HCR) strategy, the fluorescence signal can be obviously amplified...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28500632/altered-expression-of-four-mirna-mir-1238-3p-mir-202-3p-mir-630-and-mir-766-3p-and-their-potential-targets-in-peripheral-blood-from-vitiligo-patients
#18
Zhiwei Shang, Hongwen Li
Vitiligo is an acquired skin disease with pigmentary disorder. Autoimmune destruction of melanocytes is thought to be major factor in the etiology of vitiligo. miRNA-based regulators of gene expression have been reported to play crucial roles in autoimmune disease. Therefore, we attempt to profile the miRNA expressions and predict their potential targets, assessing the biological functions of differentially expressed miRNA. Total RNA was extracted from peripheral blood of vitiligo (experimental group, n = 5) and non-vitiligo (control group, n = 5) age-matched patients...
May 13, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28499468/corrigendum-to-ultrasensitive-and-selective-electrochemical-biosensor-for-detection-of-mercury-ii-ions-by-nicking-endonuclease-assisted-target-recycling-and-hybridization-chain-reaction-signal-amplification-biosens-bioelectron-94-2017-19-23
#19
https://www.readbyqxmd.com/read/28498283/synovial-sarcoma-of-peripheral-nerves-analysis-of-15-cases
#20
John S A Chrisinger, Usama I Salem, Lars-Gunnar Kindblom, Behrang Amini, Magnus Hansson, Jeanne M Meis
Synovial sarcoma of peripheral nerve (SSPN) is rare with only 26 cases reported in English. SSPN is often mistaken for a benign or malignant peripheral nerve sheath tumor (PNST) by clinicians and pathologists. Fifteen cases of SSPN were retrieved from the pathology files of 3 institutions. All tumors arose in a nerve based on imaging and/or operative findings and the diagnoses were histologically confirmed. Neuropathic symptoms predominated in 11 women and 4 men, 19- to 62-year-old (median, 39 years) with tumors involving the ulnar (5), median (3), peroneal (3) or sciatic (2) nerve, or the L4 or T1 nerve root (2)...
May 11, 2017: American Journal of Surgical Pathology
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