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https://www.readbyqxmd.com/read/28737941/unveiling-the-catalytic-role-of-b-block-histidine-in-the-n-s-acyl-shift-step-of-protein-splicing
#1
Jon I Mujika, Xabier Lopez
Protein splicing is a post-translational modification that involves the excision of a segment denoted as intein and the joining of its two flanking segments. The process is auto-catalytic, making inteins appealing for many applications in biotechnology, bioengineering, or medicine. The canonical mechanism of protein splicing is composed of four sequential steps, and is initialized by an N-S or N-O acyl shift to form a linear ester. It is well established that a histidine, the most conserved amino acid in all inteins catalyzes this initial step, even though its role remains to be understood...
July 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28737006/unrecognized-viral-infections-and-chromosome-abnormalities-as-a-cause-of-fetal-death-examination-with-fluorescence-in%C3%A2-situ-hybridization-immunohistochemistry-and-polymerase-chain-reaction
#2
Bente Ediassen Opsjøn, Svein Arne Nordbø, Christina Vogt
Fifteen to 50% of fetal deaths remain unexplained after post-mortem examination depending on inclusion criteria and classification systems. Our aim was to examine a selection of unexplained fetal deaths in order to investigate whether any common chromosome aberrations or viral infections were present. Reports from 351 fetal autopsies performed at the Department of Pathology and Medical Genetics at St. Olavs University Hospital from 2001 through 2010 were reviewed. Of these, 105 fetal deaths were classified as unexplained...
July 24, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28736786/an-enzyme-free-flow-cytometric-bead-assay-for-the-sensitive-detection-of-micrornas-based-on-click-nucleic-acid-ligation-mediated-signal-amplification
#3
Yan Qi, Liying Qiu, Wenjiao Fan, Chenghui Liu, Zhengping Li
A versatile flow cytometric bead assay (FCBA) coupled with a completely enzyme-free signal amplification mechanism is developed for the sensitive detection of microRNAs (miRNAs). This new strategy integrates click chemistry-mediated ligation chain reaction (CLCR) with hybridization chain reaction (HCR) for enzyme-free signal amplification on magnetic beads (MBs), and a flow cytometer for the robust fluorescence readout of the MBs. Firstly, target miRNA can initiate CLCR on the surface of MBs based on the click chemical ligation between dibenzocyclooctyne (DBCO)- and azide-modified single-stranded DNA (ssDNA) probes, and the amount of ligated ssDNA sequences on the MBs will be proportional to the dosage of target miRNA...
July 24, 2017: Analyst
https://www.readbyqxmd.com/read/28735477/molecular-malfeasance-mediating-myeloid-malignancies-the-genetics-of-acute-myeloid-leukemia
#4
Rebecca L King, Adam Bagg
A remarkable number of different, but recurrent, structural cytogenetic abnormalities have been observed in AML, and the 2016 WHO AML classification system incorporates numerous distinct entities associated with translocations or inversions, as well as others associated with single gene mutations into a category entitled "AML with recurrent genetic abnormalities." The AML classification is heavily reliant on cytogenetic and molecular information based on conventional genetic techniques (including karyotype, fluorescence in situ hybridization, reverse transcriptase polymerase chain reaction, single gene sequencing), but large-scale next generation sequencing is now identifying novel mutations...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28734662/transient-global-cerebral-ischemia-induces-rnf213-a-moyamoya-disease-susceptibility-gene-in-vulnerable-neurons-of-the-rat-hippocampus-ca1-subregion-and-ischemic-cortex
#5
Mika Sato-Maeda, Miki Fujimura, Sherif Rashad, Yuiko Morita-Fujimura, Kuniyasu Niizuma, Hiroyuki Sakata, Shuntaro Ikawa, Teiji Tominaga
The RING finger protein 213 (RNF213) is an important susceptibility gene for moyamoya disease (MMD) and is also implicated in other types of intracranial major artery stenosis/occlusion (ICAS); however, the role of RNF213 in the development of ICAS including MMD is unclear. The constitutive expression of the RNF213 gene is relatively weak in brain tissue, while information regarding the expression patterns of the RNF213 gene under cerebral ischemia, which is one of characteristic pathologies associated with ICAS, is currently limited...
July 19, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28731788/identification-of-osr2-transcriptional-target-genes-in-palate-development
#6
X Fu, J Xu, P Chaturvedi, H Liu, R Jiang, Y Lan
Previous studies have identified the odd-skipped related 2 (Osr2) transcription factor as a key intrinsic regulator of palatal shelf growth and morphogenesis. However, little is known about the molecular program acting downstream of Osr2 in the regulation of palatogenesis. In this study, we isolated palatal mesenchyme cells from embryonic day 12.5 (E12.5) and E13.5 Osr2(RFP/+) and Osr2(RFP/-) mutant mouse embryos and performed whole transcriptome RNA sequencing analyses. Differential expression analysis of the RNA sequencing datasets revealed that expression of 70 genes was upregulated and expression of 61 genes was downregulated by >1...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28728006/an-electrochemical-impedance-biosensor-for-hg-2-detection-based-on-dna-hydrogel-by-coupling-with-dnazyme-assisted-target-recycling-and-hybridization-chain-reaction
#7
Wei Cai, Shunbi Xie, Jin Zhang, Dianyong Tang, Ying Tang
In this work, an electrochemical impedance biosensor for high sensitive detection of Hg(2+) was presented by coupling with Hg(2+)-induced activation of Mg(2+)-specific DNAzyme (Mg(2+)-DNAzyme) for target cycling and hybridization chain reaction (HCR) assembled DNA hydrogel for signal amplification. Firstly, we synthesized two different copolymer chains P1 and P2 by modifying hairpin DNA H3 and H4 with acrylamide polymer, respectively. Subsequently, Hg(2+) was served as trigger to activate the Mg(2+)-DNAzyme for selectively cleavage ribonucleobase-modified substrate in the presence of Mg(2+)...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28723242/interspecific-and-intraspecific-hybrid-epichlo%C3%A3-species-symbiotic-with-the-north-american-native-grass-poa-alsodes
#8
Tatsiana Shymanovich, Nikki D Charlton, Ashleigh M Musso, Jonathan Scheerer, Nadja B Cech, Stanley H Faeth, Carolyn A Young
The endophyte presence and diversity in natural populations of Poa alsodes were evaluated along a latitudinal transect from the southern distribution range in North Carolina to New York. Two distinct Epichloë hybrid taxa were identified from 23 populations. Each taxon could easily be distinguished by polymerase chain reaction (PCR) genotyping with primers designed to mating type genes and alkaloid biosynthesis genes that encode key pathway steps for ergot alkaloids, indole-diterpenes, lolines, and peramine...
June 26, 2017: Mycologia
https://www.readbyqxmd.com/read/28723114/simultaneous-imaging-of-three-tumor-related-mrnas-in-living-cells-with-a-dna-tetrahedron-based-multicolor-nanoprobe
#9
Song Wang, Mengchan Xia, Jie Liu, Sichun Zhang, Xinrong Zhang
We constructed a DNA tetrahedron based multicolor nanoprobe, which could simultaneously imaging of three tumor-related mRNAs in living cells through fluorescence restoration caused by competitive chain replacement reaction. The oligonucleotides used to construct the tetrahedron were extended by adding three 21-base recognition sequences modified with different fluorophores (FAM, Cy3, and Cy5) in the 5' end. Three 11-base complementary sequences modified with quencher (BHQ1 for FAM and BHQ2 for Cy3 and Cy5) were hybridized with the recognition sequences to quench the fluorescence...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28710458/design-of-carbon-dots-photoluminescence-through-organo-functional-silane-grafting-for-solid-state-emitting-devices
#10
Kazumasa Suzuki, Luca Malfatti, Masahide Takahashi, Davide Carboni, Fabrizio Messina, Yasuaki Tokudome, Masanori Takemoto, Plinio Innocenzi
Advanced optical applications of fluorescent carbon dots (C-dots) require highly integrated host-guest solid-state materials with a careful design of C-dots - matrix interface to control the optical response. We have developed a new synthesis based on the grafting of an organo-functional silane (3-glycidyloxypropyltrimethoxysilane, GPTMS) on amino-functionalized C-dots, which enables the fabrication of highly fluorescent organosilica-based hybrid organic-inorganic films through sol-gel process. The GPTMS grafting onto C-dots has been achieved via an epoxy-amine reaction under controlled conditions...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709088/integrated-amplified-aptasensor-with-in-situ-precise-preparation-of-copper-nanoclusters-for-ultrasensitive-electrochemical-detection-of-microrna-21
#11
Yijia Wang, Xinying Zhang, Lang Zhao, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang
MicroRNA 21 (MIR21) has garnered much attention in recent years as an important disease biomarker. The detection of it in human system shows great significance for the healthy evaluation and major diseases early detection. Herein, a novel approach tactfully manipulates the in-situ precise preparation of copper nanoclusters on overlapping Y-shaped ds-DNA for MIR21 analysis were developed in the proposed integrated aptasensor. In the presence of target MIR21, overlapping Y-shaped ds-DNA was constructed on electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28701884/cis-prenyltransferase-interacts-with-a-nogo-b-receptor-homolog-for-dolichol-biosynthesis-in-panax-ginseng-meyer
#12
Ngoc Quy Nguyen, Sang-Choon Lee, Tae-Jin Yang, Ok Ran Lee
BACKGROUND: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28695686/imprint-cytology-of-clear-cell-sarcoma-like-tumor-of-the-gastrointestinal-tract-in-the-small-intestine-a-case-report
#13
Takashi Kato, Shin Ichihara, Hiroko Gotoda, Shunji Muraoka, Terufumi Kubo, Shintaro Sugita, Tadashi Hasegawa
Clear cell sarcoma-like tumor of the gastrointestinal tract (CCSLGT) is an extremely rare malignant neoplasm in the digestive tract. Its cytomorphologic features have never previously been reported. Here, we describe a case of CCSLGT, including its cytologic examination findings. A 47-year-old woman presented with a mass in the small intestine, which was resected and sent for imprint cytology. Imprint smears revealed tumor cells with light eosinophilic or clear cytoplasm in a necrotic background. Many of the tumor cells were arranged in a perivascular growth with a pseudopapillary formation, and there were some non-neoplastic osteoclast-like giant cells...
July 11, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28693295/germline-dna-copy-number-variations-as-potential-prognostic-markers-for-non-muscle-invasive-bladder-cancer-progression
#14
Yoshiaki Yamamoto, Yutaka Suehiro, Atomu Suzuki, Ryosuke Nawata, Yoshihisa Kawai, Ryo Inoue, Hiroshi Hirata, Hiroaki Matsumoto, Takahiro Yamasaki, Kohsuke Sasaki, Hideyasu Matsuyama
Accumulating evidence has suggested that germline DNA copy number variations (CNVs) affect various disorders, including human malignancies. However, the significance of CNVs in non-muscle invasive bladder cancer (NMIBC) remains unclear. The purpose of the present study was to identify the role of CNVs in NMIBC. Array comparative genomic hybridization (CGH) analysis was performed to search for candidate CNVs associated with NMIBC susceptibility. Quantitative polymerase chain reaction was carried out to evaluate CNVs associated with patient outcome in 189 NMIBC cases...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28693234/relapsed-anaplastic-lymphoma-kinase-positive-large-b-cell-lymphoma-expressed-cluster-of-differentiation-4-and-cytokeratin-an-initially-misdiagnosed-case-corrected-by-immunoglobulin-%C3%AE%C2%BA-locus-gene-rearrangement-detection
#15
Yunfei Shi, Xianghong Li, Yuqin Song, Lixin Zhou, Qin Feng, Ping Wang, Chen Zhang, Weiping Liu, Yanhua Bai, Yumei Lai
Anaplastic lymphoma kinase (ALK)-positive large B-cell lymphoma (LBCL) is a rare lymphoma subtype. The present study investigated a refractory nodal ALK-positive LBCL case in a 28-year-old Chinese male. It was initially misdiagnosed as ALK-positive anaplastic large cell lymphoma; however, the patient's lesions relapsed and spread widely following a short remission for chemotherapy and the patient succumbed to the disease 3 months' post-autologous stem cell transplantation; thus, a revision was performed. Histologically, the tumor cells exhibited a characteristic immunoblastic morphology with marked cellular pleomorphism...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28693135/c59t-mutation-in-exon-2-of-monocytic-leukemia-associated-antigen-34-gene-indicates-a-high-risk-of-recurrence-of-acute-myeloid-leukemia
#16
Bo Lei, Yinxia Chen, Aili He, Jing Luo, Pengyu Zhang, Fuling Zhou, Jie Liu, Xin Meng, Jing Wang, Wanggang Zhang
Monocytic leukemia-associated antigen-34 (MLAA-34) is a novel monocytic leukemia-associated antigen and a candidate oncogene. The aim of the present study was to investigate the involvement of the MLAA-34 gene in acute myeloid leukemia (AML). MLAA-34 expression level, chromosome location, gene copy number and single nucleotide polymorphisms (SNPs) of 40 patients with AML and 5 healthy volunteers were analyzed by reverse transcription-polymerase chain reaction, fluorescence in situ hybridization and DNA sequencing...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28688371/6-2-fluorotelomer-carboxylic-acid-6-2-ftca-exposure-induces-developmental-toxicity-and-inhibits-the-formation-of-erythrocytes-during-zebrafish-embryogenesis
#17
Guohui Shi, Qianqian Cui, Yitao Pan, Nan Sheng, Yong Guo, Jiayin Dai
Saturated fluorotelomer carboxylic acids (FTCAs) are intermediates in the degradation of fluorotelomer alcohols (FTOHs) to perfluorinated carboxylic acids (PFCAs). Recent studies have detected FTCAs in precipitation, surface waters, and wildlife, but few studies have focused on their toxicity. In this study, zebrafish embryos were exposed to different concentrations of 6:2 FTCA (0, 4, 8, and 12mg/L) from 6 to 120h post-fertilization (hpf) to investigate its developmental toxicity. Results showed that 6:2 FTCA exposure decreased the hatching and survival percentages, reduced the heart rate, and increased the malformation of zebrafish embryos...
June 27, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28687930/spatiotemporal-expression-patterns-of-clusterin-in-the-mouse-inner-ear
#18
Seokwon Lee, Jeong-Oh Shin, Borum Sagong, Un-Kyung Kim, Jinwoong Bok
Clusterin (CLU) is an extracellular chaperone protein that is implicated in diverse physiological and pathophysiological cellular processes. CLU expression is upregulated in response to cellular stress and under certain conditions, such as neurodegenerative disease and cancer. CLU primarily functions as a chaperone that exerts cytoprotective effects by removing cellular debris and misfolded proteins and also acts as a signaling molecule that regulates pro-survival pathways. Deafness is caused by genetic factors and various extrinsic insults, including ototoxic drugs, exposure to loud sounds and aging...
July 8, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28677087/cloning-of-bbtv-banana-bunchy-top-virus-components-and-screening-of-bbtv-using-functionalized-gold-nanoparticles
#19
P Kumar, V Arun, T S Lokeswari
Banana bunchy top virus (BBTV) affects all varieties of banana plants and causes heavy economic loss in most of the banana cultivating areas. The BBTV genome comprises of six DNA components; in this study, we have cloned the six BBTV-DNA components from one of the BBTV-infected plants (Tri-8) and were submitted to GenBank. Analysis of the BBTV DNA-R component showed that it belonged to south Pacific group. Resistance against BBTV has not been observed so far in banana plants and removal and killing of the infected plants has been routinely practiced...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28672982/upregulation-of-mir-125b-is-associated-with-poor-prognosis-and-trastuzumab-resistance-in-her2-positive-gastric-cancer
#20
Minghua Sui, Aihong Jiao, Huiyuan Zhai, Yan Wang, Yong Wang, Dengjun Sun, Peng Li
Gastric cancer is one of the most common types of human cancer associated with a poor prognosis. MicroRNAs (miRs), a class of non-coding RNAs that are 18-25 nucleotides in length, act as key regulators in gene expression, and have been implicated in various human cancer types. miR-125b has been implicated in the malignant progression of gastric cancer. However, the association between miR-125b expression, clinicopathological characteristics and trastuzumab resistance in human epidermal growth factor receptor 2 (HER2)-positive gastric cancer remains unclear...
July 2017: Experimental and Therapeutic Medicine
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