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https://www.readbyqxmd.com/read/29340242/a-diagnostic-model-for-minimal-change-disease-based-on-biological-parameters
#1
Hanyu Zhu, Qiuxia Han, Dong Zhang, Yong Wang, Jing Gao, Wenjia Geng, Xiaoli Yang, Xiangmei Chen
Background: Minimal change disease (MCD) is a kind of nephrotic syndrome (NS). In this study, we aimed to establish a mathematical diagnostic model based on biological parameters to classify MCD. Methods: A total of 798 NS patients were divided into MCD group and control group. The comparison of biological indicators between two groups were performed with t-tests. Logistic regression was used to establish the diagnostic model, and the diagnostic value of the model was estimated using receiver operating characteristic (ROC) analysis...
2018: PeerJ
https://www.readbyqxmd.com/read/29334611/understanding-complex-tribofilms-by-means-of-h3bo3-b2o3-model-glasses
#2
Fabiana Spadaro, Antonella Rossi, Shivaprakash N Ramakrishna, Emmanuel Lainé, Philip Woodward, Nicholas D Spencer
The discovery of the spontaneous reaction of boric oxides with moisture in the air to form lubricious H3BO3 films has led to great interest in the tribology of boron compounds in general. Despite this, a study of the growth kinetics of H3BO3 on a B2O3 substrate under controlled relative humidity (RH), has not yet been reported in the literature. Here, we describe the tribological properties of H3BO3-B2O3 glass systems after aging under controlled relative humidity over different lengths of time. A series of tribological tests has been performed applying a normal load of 15 N, at both room temperature and 100°C in Yubase 4 oil...
January 15, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29331744/urinary-metabolomics-study-the-mechanism-of-taohong-siwu-decoction-intervention-in-acute-blood-stasis-model-rats-based-on-liquid-chromatography-coupled-to-quadrupole-time-of-flight-mass-spectrometry
#3
Xiaosong Zhang, Pengling Li, Yongli Hua, Peng Ji, Wanling Yao, Qi Ma, Ziwen Yuan, Yanqiao Wen, Chaoxue Yang, Yanming Wei
Taohong Siwu Decoction (TSD) is a classic prescription in traditional Chinese medicine and is widely used to promote blood circulation to remove blood stasis. However, the effect mechanisms are not yet well understood. Here, a urinary metabolomic approach based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC/Q-TOF-MS) was conducted to explore the changes in the endogenous metabolites and to assess the integral efficacy of TSD on acute blood stasis model rats. Then, parameters for hemorheology and coagulation functions were detected...
January 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29330728/analysis-of-plasma-tenascin-c-in-post-hcv-cirrhosis-a-prospective-study
#4
Jennifer H Benbow, April D Elam, Krista L Bossi, Danae L Massengill, Elizabeth Brandon-Warner, William E Anderson, Catherine R Culberson, Mark W Russo, Andrew S deLemos, Laura W Schrum
BACKGROUND AND AIM: Hepatitis C virus (HCV)-related cirrhosis, one of the most common etiologies of liver cirrhosis in the Western world, is a risk factor for hepatocellular carcinoma. To confirm and improve current effectiveness of screening and prognosis of patients with established cirrhosis, a credible, simple plasma biomarker is needed. Hepatic stellate cell activation, a pivotal event in cirrhosis development, results in increased secretion of extracellular matrix proteins, including tenascin-C (TnC)...
January 12, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29324755/hiv-1-co-receptor-tropism-and-liver-fibrosis-in-hiv-infected-patients
#5
Annalisa Saracino, Alessandro Cozzi-Lepri, Milensu Shanyinde, Francesca Ceccherini Silberstein, Silvia Nozza, Antonio Di Biagio, Giovanni Cassola, Giuseppe Bruno, Maria Capobianchi, Massimo Puoti, Laura Monno, Antonella d'Arminio Monforte
BACKGROUND: In vitro, gp120 of both X4 and R5 HIV-1 strains activates human hepatic stellate cells, but if it can promote liver fibrosis in vivo is unknown. We aimed to evaluate if patients carrying X4 or R5 strains have a different liver fibrosis (LF) progression over time. METHODS: A total of 1,137 HIV-infected patients in ICONA cohort (21% females, 7% HCV co-infected) with an available determination of HIV-1 co-receptor tropism (CRT), a Fibrosis-4 Index for Liver Fibrosis (FIB-4) <3...
2018: PloS One
https://www.readbyqxmd.com/read/29322134/label-free-detection-of-fibrinogen-based-on-the-fibrinogen-enhanced-peroxidase-activity-of-a-fibrinogen-hemin-composite
#6
Tingting Hou, Yuanfu Zhang, Tao Wu, Meifeng Wang, Yinghong Zhang, Rui Li, Lei Wang, Qingwang Xue, Shuhao Wang
A simple, label-free colorimetric method for the determination of fibrinogen (Fib) in plasma is presented. In this work, it was observed that Fib interacted with hemin to form a hemin-Fib composite. Because Fib prevented hemin from the formation of m-oxo-dimers, the hemin-Fib composite possesses excellent peroxidase-like activity. Importantly, the peroxidase-like activity of Fib-hemin increased with the increase in the Fib. This allows us to utilize the H2O2-ABTS colorimetric system for the quantitative analysis of Fib...
January 11, 2018: Analyst
https://www.readbyqxmd.com/read/29320552/first-report-of-occult-hepatitis-b-infection-among-art-na%C3%A3-ve-hiv-seropositive-individuals-in-maputo-mozambique
#7
Awa Abdul Carimo, Eduardo Samo Gudo, Cremildo Maueia, Nédio Mabunda, Lúcia Chambal, Adolfo Vubil, Ana Flora, Francisco Antunes, Nilesh Bhatt
BACKGROUND: The prevalence of hepatitis B virus (HBV) infection and human immunodeficiency virus (HIV) infection in Mozambique is one of the highest in the world, though in spite of this the prevalence of occult hepatitis B infection (OBI) is unknown. OBJECTIVES: This study was conducted with the aim to investigate the prevalence of OBI and frequency of isolated hepatitis B core antibody (anti-HBc alone) among antiretroviral (ART) naïve HIV-positive patients in Mozambique...
2018: PloS One
https://www.readbyqxmd.com/read/29318450/the-utility-of-noninvasive-scores-in-assessing-the-prevalence-of-nonalcoholic-fatty-liver-disease-and-advanced-fibrosis-in-type-1-diabetic-patients
#8
Amandeep Singh, Phuc Le, Rocio Lopez, Naim Alkhouri
GOALS: The aim of our study is to assess the prevalence of nonalcoholic fatty liver disease (NAFLD) and advanced hepatic fibrosis in patients with type 1 diabetes (T1D) using simple noninvasive scores. BACKGROUND: There is paucity of data on the prevalence of NAFLD in T1D. Moreover, T1D could be a risk factor for advanced disease in NAFLD patients. STUDY: Using ICD-9 codes, all patients with the diagnosis of T1D were reviewed and a retrospective chart analysis was carried out on 23,314 patients between the ages of 18 and 80...
January 9, 2018: Hepatology International
https://www.readbyqxmd.com/read/29315641/novel-scores-combining-afp-with-non-invasive-markers-for-prediction-of-liver-fibrosis-in-chronic-hepatitis-c-patients
#9
Mohamed Gamil, Mohamed Alboraie, Mohammad El-Sayed, Aisha Elsharkawy, Noha Asem, Tamer Elbaz, Mohammad Mohey, Bahaa Abbas, Mai Mehrez, Gamal Esmat
BACKGROUND: Serum levels of alpha-fetoprotein (AFP) were reported to increase in patients with significant or advanced hepatic fibrosis. Combination of non-invasive tests decreases the use of liver biopsy in large proportion of chronic HCV patients. AIM: To compare and combine AFP with commonly used non-invasive fibrosis tests in novel scores for prediction of different stages of hepatic fibrosis. PATIENTS AND METHODS: Six hundred and fifty two treatment naïve chronic hepatitis C patients were enrolled...
January 6, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29314613/validation-and-comparison-of-seventeen-noninvasive-models-for-evaluating-liver-fibrosis-in-chinese-hepatitis-b-patients
#10
Minhui Dong, Jingwen Wu, Xueping Yu, Jing Li, Sisi Yang, Xun Qi, Richeng Mao, Yongmei Zhang, Jie Yu, Haoxiang Zhu, Feifei Yang, Yanli Qin, Jiming Zhang
BACKGROUND & AIMS: To avoid liver biopsy, many noninvasive models comprised of serum markers for liver fibrosis assessment have been developed. Given that most of them were developed in hepatitis C cohorts and few of them have been validated in Chinese hepatitis B patients, we aim to conduct this validation and compare their diagnostic accuracies in such a population. METHODS: A total of 937 HBV infected patients who underwent liver biopsy were included in this single-center retrospective study...
January 3, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29310350/relationship-between-skeletal-muscle-mass-and-liver-fibrosis-markers-for-patients-with-hepatitis-c-virus-related-liver-disease
#11
Ryo Takata, Hiroki Nishikawa, Hirayuki Enomoto, Yoshinori Iwata, Akio Ishii, Yuho Miyamoto, Noriko Ishii, Yukihisa Yuri, Kunihiro Hasegawa, Chikage Nakano, Takashi Nishimura, Kazunori Yoh, Nobuhiro Aizawa, Yoshiyuki Sakai, Naoto Ikeda, Tomoyuki Takashima, Hiroko Iijima, Shuhei Nishiguchi
We aimed to elucidate the relationship between serum liver fibrosis markers (Mac-2 binding protein glycosylation isomer (M2BPGi), FIB-4 index, aspartate aminotransferase to platelet ratio index and hyaluronic acid), and skeletal muscle mass and to investigate factors linked to skeletal muscle mass loss (SMML) in patients with chronic hepatitis C (CHC, n = 277, median age = 64 years). We defined patients with psoas muscle index [PMI, sum of bilateral psoas muscle mass calculated by manual trace method at the lumber 3 level on the computed tomography images divided by height squared (cm/m)] less than 6...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29305512/molecular-epidemiology-of-dairy-cattle-associated-escherichia-coli-carrying-blactx-m-genes-in-washington-state
#12
Josephine A Afema, Sara Ahmed, Thomas E Besser, Lisa P Jones, William M Sischo, Margaret A Davis
An increase in prevalence of commensal Escherichia coli carrying blaCTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 blaCTX-M-positive and 126 blaCTX-M-negative isolates for multi-locus sequence typing (MLST) and antibiotic resistance phenotyping from E. coli obtained during a previous study. For 99 isolates we also determined the blaCTX-M alleles using PCR and sequencing and identified the replicon types of blaCTX-M carrying plasmids...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29305503/elucidating-waterborne-pathogen-presence-and-aiding-source-apportionment-in-an-impaired-stream
#13
Jennifer Weidhaas, Angela Anderson, Rubayat Jamal
Fecal indicator bacteria (FIB) are the basis for water quality regulations and are considered proxies for waterborne pathogens when conducting human health risk assessments. Direct detection of pathogens in water and simultaneous identification of the source of fecal contamination is possible with microarrays, circumventing drawbacks to FIB approaches. A multi-gene target microarray was used to assess the prevalence of waterborne pathogens in a fecally impaired, mixed use watershed. Results indicate that fecal coliforms have improved substantially in the watershed since its listing as a 303(d) impaired stream in 2002 and are now near United States recreational water criteria standards...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29304855/prognostic-value-of-coagulation-tests-for-in-hospital-mortality-in-patients-with-traumatic-brain-injury
#14
Qiang Yuan, Jian Yu, Xing Wu, Yi-Rui Sun, Zhi-Qi Li, Zhuo-Ying Du, Xue-Hai Wu, Jin Hu
BACKGROUND: Coagulopathy is commonly observed after traumatic brain injury (TBI). However, it is not known whether using the standard independent predictors in conjunction with coagulation tests would improve their prognostic value. We determined the incidence of TBI-associated coagulopathy in patients with isolated TBI (iTBI), evaluated the prognostic value of coagulation tests for in-hospital mortality, and tested their predictive power for in-hospital mortality in patients with iTBI...
January 5, 2018: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/29303701/increased-levels-of-serum-histone-h4-and-activated-protein-c-in-patients-with-active-rheumatoid-arthritis
#15
Wanchan Peng, Xianghui Liang, Yong Zhong, Yaou Zhou, Litao Hu, Wei Wang
OBJECTIVES: We aimed to examine the levels of serum H4 and activated protein C (APC) in rheumatoid arthritis (RA) and other autoimmune conditions, and investigate the associations between H4 or APC levels and disease activity indicators in RA. METHODS: Serum H4 and APC distribution was examined in samples from patients with RA, systemic lupus erythematosus (SLE), polymyositis (PM), and ankylosing spondylitis (AS), as well as in samples from healthy controls, using commercial ELISA kits...
December 27, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29301542/monitoring-of-poyang-lake-water-for-sewage-contamination-using-human-enteric-viruses-as-an-indicator
#16
Hui Zhu, Fang Yuan, Zhaokang Yuan, Rong Liu, Fei Xie, Ling Huang, Xiaojun Liu, Xiaoqing Jiang, Jian Wang, Qunying Xu, Zhiqiang Shen, Donghan Liu, Ronghao Zhang, Yuanan Lu
BACKGROUND: Recreational water contaminated with fecal pollution poses a great public health concern, as fecal waste may cause serious waterborne illnesses. Current recreational water standards using fecal indicator bacteria (FIB) have their limitations for human protection especially in developing countries such as China. METHODS: To explore the potential use of enteric viruses as a potential indicator of fecal contamination, four viruses: norovirus geno-groups I and II, enteroviruses, and adenoviruses were tested in this study using molecular detection methods and sensitive RT-PC developed in the University of Hawaii...
January 5, 2018: Virology Journal
https://www.readbyqxmd.com/read/29300172/fabrication-of-nanopores-and-nanoslits-with-feature-sizes-down-to-5-nm-by-wet-etching-method
#17
Qi Chen, Yifan Wang, Tao Deng, Zewen Liu
This paper presents an improved three-step wet etching (I-TSWE) method for the fabrication of single-crystal silicon (SCS) nanopores and nanoslists. A diffusion model was built to analyze the influence of the color-based feedback mechanism on the final pore size. Reference structures were added aside normal pore patterns, to obtain a more precise control of the pore size during the pore opening process. By using this method, square nanopores with the minimum size of 8 nm × 8 nm, rectangle nanopores and nanoslits with feature sizes down to 5 nm were successfully obtained...
January 4, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29295657/comparing-coagulation-activity-of-selaginella-tamariscina-before-and-after-stir-frying-process-and-determining-the-possible-active-constituents-based-on-compositional-variation
#18
Qian Zhang, Ya-Li Wang, Die Gao, Liang Cai, Yi-Yao Yang, Yuan-Jia Hu, Feng-Qing Yang, Hua Chen, Zhi-Ning Xia
CONTEXT: Selaginella tamariscina (P. Beauv.) Spring (Selaginellaceae) (ST) has been widely used in China as a medicine for improving blood circulation. However, its processed product, S. tamariscina carbonisatus (STC), possesses opposite haemostatic activity. OBJECTIVE: To comprehensively evaluate the activity of ST and STC on physiological coagulation system of rats, and seek potential active substances accounting for the activity transformation of ST during processing...
December 2018: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29289855/two-novel-approaches-to-study-arthropod-anatomy-by-using-dualbeam-fib-sem
#19
Andrea Di Giulio, Maurizio Muzzi
Transmission Electron Microscopy (TEM) has always been the conventional method to study arthropod ultrastructure, while the use of Scanning Electron Microscopy (SEM) was mainly devoted to the examination of the external cuticular structures by secondary electrons. The new generation field emission SEMs are capable to generate images at sub-cellular level, comparable to TEM images employing backscattered electrons. The potential of this kind of acquisition becomes very powerful in the dual beam FIB/SEM where the SEM column is combined with a Focused Ion Beam (FIB) column...
December 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29285715/gene-mutations-and-clinical-phenotypes-in-15-chinese-children-with-cryopyrin-associated-periodic-syndrome-caps
#20
Caifeng Li, Xiaohua Tan, Junmei Zhang, Shipeng Li, Wenxiu Mo, Tongxin Han, Weiying Kuang, Yifang Zhou, Jianghong Deng
The aim of our study is to explore the features of clinical manifestations and genetic mutations in Chinese CAPS patients. Fifteen confirmed patients with CAPS were enrolled. The onset time ranges from 2 days after birth to 6 years and 1 month. Recurrent urticaria rash (93.3%) with fever (100%) were two dominant characteristics of these patients that were presented as either acute or chronic process. Systemic involvements were found in all patients except for one with only rash and fever. The top three symptoms were fever (100%), rash (93...
December 2, 2017: Science China. Life Sciences
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