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https://www.readbyqxmd.com/read/28430425/environmental-growth-of-enterococci-and-escherichia-coli-in-feedlot-catch-basins-and-a-constructed-wetland-in-the-absence-of-fecal-input
#1
Lisa Tymensen, Calvin W Booker, Sherry J Hannon, Shaun R Cook, Rahat Zaheer, Ron Read, Tim A McAllister
Population structures of fecal indicator bacteria (FIB) isolated from catch basins, a constructed wetland, and feces from a beef cattle feedlot were compared over a two-year period. Enterococcus hirae accounted for 92% of the fecal isolates, whereas secondary environments were characterized by greater relative abundance of environmentally adapted species including Enterococcus casseliflavus. While enterococci densities in the catch basins and wetland were similar under wet and drought conditions, E. hirae predominated during rainy periods while E...
April 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28425454/association-of-serum-autotaxin-levels-with-liver-fibrosis-in-patients-with-chronic-hepatitis-c
#2
Tomoo Yamazaki, Satoru Joshita, Takeji Umemura, Yoko Usami, Ayumi Sugiura, Naoyuki Fujimori, Soichiro Shibata, Yuki Ichikawa, Michiharu Komatsu, Akihiro Matsumoto, Koji Igarashi, Eiji Tanaka
Metabolized by liver sinusoidal endothelial cells, autotaxin (ATX) is a secreted enzyme considered to be associated with liver damage. We sought to clarify the diagnostic ability of ATX for liver fibrosis in 593 biopsy-confirmed hepatitis C virus (HCV)-infected patients. The diagnostic accuracy of ATX was compared with clinical parameters and the established fibrosis biomarkers Wisteria floribunda agglutinin-positive Mac-2-binding protein, FIB-4 index, AST-to-platelet ratio, and Forn's index. Median ATX levels were consistently higher in female controls and patients than in their male counterparts (P < 0...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425016/a-simple-bedside-blood-test-fibrofast-fib-5-is-superior-to-fib-4-index-for-the-differentiation-between-non-significant-and-significant-fibrosis-in-patients-with-chronic-hepatitis-c
#3
G Shiha, S Seif, A Eldesoky, M Elbasiony, R Soliman, A Metwally, K Zalata, N Mikhail
INTRODUCTION: A simple non-invasive score (Fibrofast, FIB-5) was developed using five routine laboratory tests (ALT, AST, alkaline phosphatase, albumin and platelets count) for the detection of significant hepatic fibrosis in patients with chronic hepatitis C. The FIB-4 index is a non-invasive test for the assessment of liver fibrosis, and a score of ≤1.45 enables the correct identification of patients who have non-significant (F0-1) from significant fibrosis (F2-4), and could avoid liver biopsy...
April 19, 2017: Hepatology International
https://www.readbyqxmd.com/read/28424665/ca-similichlamydia-in-epitheliocystis-co-infection-of-gilthead-seabream-gills-unique-morphological-features-of-a-deep-branching-chlamydial-family
#4
Helena M B Seth-Smith, Pantelis Katharios, Nancy Dourala, José M Mateos, Alexander G J Fehr, Lisbeth Nufer, Maja Ruetten, Maricruz Guevara Soto, Lloyd Vaughan
The Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) bacterial superphylum constitutes a broad range of organisms with an intriguing array of ultrastructural morphologies, including intracellular membranes and compartments and their corresponding complex genomes encoding these forms. The phylum Chlamydiae are all obligate intracellular bacteria and, although much is already known of their genomes from various families and how these regulate the various morphological forms, we know remarkably little about what is likely the deepest rooting clade of this phylum, which has only been found to contain pathogens of marine and fresh water vertebrates...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28422310/adult-onset-cystic-fibrosis-liver-disease-diagnosis-and-characterization-of-an-underappreciated-entity
#5
Christopher Koh, Sasan Sakiani, Pallavi Surana, Xiongce Zhao, Jason Eccleston, David E Kleiner, David Herion, T Jake Liang, Jay H Hoofnagle, Milica Chernick, Theo Heller
BACKGROUND & AIMS: Cystic fibrosis liver disease (CFLD), a leading cause of death in cystic fibrosis (CF), is mostly described in pediatric populations. Adult-onset CFLD lacks sufficient characterization and diagnostic tools. METHODS: A cohort of CF patients without CFLD during childhood were followed for up to 38 years with serologic testing, imaging, and noninvasive fibrosis markers. Historical CFLD diagnostic criteria were compared with a newly proposed CFLD criteria...
April 19, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28419722/role-of-monocyte-tissue-factor-on-patients-with-non-small-cell-lung-cancer
#6
Chaosheng Deng, Shuang Wu, Lina Zhang, Minxia Yang, Qichang Lin, Qiang Xie, Haibo Ding, Ningfang Lian, Shaoyong Gao, Yunjian Huang, Yongxu Jin
Background To examine the expression of D-dimer, fibrinogen (FIB), leukocyte, C-reactive protein (CRP) and tissue factor (TF) released from monocyte in non-small cell lung cancer (NSCLC) patients with or without venous thromboembolism (VTE) and analyze the correlation, to explore the possible mechanisms. Methods Seventy two patients confirmed the diagnosis of lung cancer, among whom 10 with VTE were enrolled into the study from November 2012 to January 2014 in the First Affiliated Hospital of Fujian Medical University, 30 healthy subjects were also enrolled as the control group...
April 17, 2017: Clinical Respiratory Journal
https://www.readbyqxmd.com/read/28416464/microbiological-quality-of-water-in-a-city-with-persistent-and-recurrent-waterborne-diseases-under-tropical-sub-rural-conditions-the-case-of-kikwit-city-democratic-republic-of-the-congo
#7
Alexis B Nienie, Periyasamy Sivalingam, Amandine Laffite, Patience Ngelinkoto, Jean-Paul Otamonga, Alphonse Matand, Crispin K Mulaji, Emmanuel M Biey, Pius T Mpiana, John Poté
The availability of safe drinking water in sub-Saharan countries remains a major challenge because poor sanitation has been the cause of various outbreaks of waterborne disease due to the poor microbiological quality of water used for domestic purposes. The faecal indicator bacteria (FIB) used in the present study included Escherichia coli (E. coli) and Enterococcus (ENT). FIB and aerobic mesophilic bacteria (AMB) were quantified during July 2015 (dry season) and November 2015 (rainy season) in order to assess the quality of drinking water from wells (n=3; P1-P3), and two rivers, the River Lukemi (RLK, n=3) and River Luini (RLN, n=2) in the city of Kikwit, which is located in the province of Kwilu in the Democratic Republic of the Congo...
March 30, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28415736/the-gamma-glutamyl-transpeptidase-to-platelet-ratio-for-non-invasive-assessment-of-liver-fibrosis-in-patients-with-chronic-hepatitis-b-and-non-alcoholic-fatty-liver-disease
#8
Qiang Li, Chuan Lu, Weixia Li, Yuxian Huang, Liang Chen
BACKGROUND/AIM: The gamma-glutamyl transpeptidase-to-platelet ratio (GPR) is a novel serum model, which was reported more accurate than aspartate transaminase-to-platelet ratio index (APRI) and fibrosis index based on four factors (FIB-4) for diagnosing significant fibrosis and cirrhosis in HBV mono-infection in West Africa. We aimed to evaluate the diagnostic performance of GPR for liver fibrosis in patients with chronic hepatitis B (CHB) and non-alcoholic fatty liver disease (NAFLD)...
March 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414918/frogs-host-faecal-bacteria-typically-associated-with-humans
#9
Karen Gibb, Xavier Schobben, Keith Christian
Tree frogs commonly access drinking water tanks, which may have human health implications. Although amphibians might not be expected to host mammalian faecal indicator bacteria (FIB), it is possible that they may have human FIB on their skin after exposure to human waste. We collected faeces and skin wash from green tree frogs (Litoria caerulea) from a natural environment, a suburban site, and a suburban site near a creek occasionally contaminated with sewage effluent. We used molecular techniques to test for FIB that are routinely used to indicate human faecal contamination...
April 17, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28414467/variation-of-bacterial-communities-with-water-quality-in-an-urban-tropical-catchment
#10
Jean Pierre Nshimyimana, Adam Joshua Ehrich Freedman, Peter Shanahan, Lloyd C H Chua, Janelle Renee Thompson
A major challenge for assessment of water quality in tropical environments is the natural occurrence and potential growth of Fecal Indicator Bacteria (FIB). To gain a better understanding of the relationship between measured levels of FIB and the distribution of sewage-associated bacteria including potential pathogens in the tropics this study compared the abundance of FIB (Total coliforms and E. coli) and the Bacteroidales (HF183 marker) with bacterial community structure determined by next-generation amplicon sequencing...
April 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28414057/non-invasive-assessment-of-liver-fibrosis-in-patients-with-hbv-related-chronic-liver-disease-undergoing-antiviral-treatment-a-preliminary-study
#11
Cristina Stasi, Elena Salomoni, Umberto Arena, Giampaolo Corti, Paolo Montalto, Filippo Bartalesi, Fabio Marra, Giacomo Laffi, Stefano Milani, Anna Linda Zignego, Massimo Pinzani
In chronic hepatitis B (CHB) patients, fibrosis assessment during antiviral treatment is a key step in the clinical management. Aim of this study was to evaluate the performance of elastography in assessing fibrosis stage in CHB before and after two years of nucleoside/nucleotide analogues (NUC) treatment in comparison with indirect serum markers. CHB diagnosis was made according to standard criteria. A clinical and virological evaluation was performed at baseline and again at 3, 6, 9, 12 18, and 24 months during treatment...
April 13, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28411821/electrochemical-discrimination-of-mints-the-last-chinese-emperors-kuang-hs%C3%A3-and-hs%C3%A3-an-t-ung-monetary-unification
#12
Antonio Doménech-Carbó, María Teresa Doménech-Carbó, Elena Montagna, Carla Álvarez-Romero, Yu Lee
An electrochemical methodology for discriminating monetary emissions, a recurrent problem in much archaeological studies, is introduced. The method is based on the record of voltammetric signatures of cuprite and tenorite corrosion products in the patina using a minimally invasive nanosampling following the voltammetry of immobilized particles methodology. A model for the depth variation of voltammetric electrochemical parameters characterizing the composition of the corrosion patinas is presented. This model permits to rationalize electrochemical data and discriminate different monetary emissions...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28410379/new-insights-into-valve-related-intramural-and-intracellular-bacterial-diversity-in-infective-endocarditis
#13
Andreas Oberbach, Nadine Schlichting, Stefan Feder, Stefanie Lehmann, Yvonne Kullnick, Tilo Buschmann, Conny Blumert, Friedemann Horn, Jochen Neuhaus, Ralph Neujahr, Erik Bagaev, Christian Hagl, Maximilian Pichlmaier, Arne Christian Rodloff, Sandra Gräber, Katharina Kirsch, Marcus Sandri, Vivek Kumbhari, Armirhossein Behzadi, Amirali Behzadi, Joao Carlos Correia, Friedrich Wilhelm Mohr, Maik Friedrich
AIMS: In infective endocarditis (IE), a severe inflammatory disease of the endocardium with an unchanged incidence and mortality rate over the past decades, only 1% of the cases have been described as polymicrobial infections based on microbiological approaches. The aim of this study was to identify potential biodiversity of bacterial species from infected native and prosthetic valves. Furthermore, we compared the ultrastructural micro-environments to detect the localization and distribution patterns of pathogens in IE...
2017: PloS One
https://www.readbyqxmd.com/read/28409147/incidence-and-predictors-of-advanced-liver-fibrosis-by-a-validated-serum-biomarker-in-liver-transplant-recipients
#14
Mamatha Bhat, Kathleen C Rollet-Kurhajec, Aparna Bhat, Amanda Farag, Marc Deschenes, Philip Wong, Peter Ghali, Giada Sebastiani
Background and Aims. Serum fibrosis biomarkers have shown good accuracy in the liver transplant (LT) population. We employed a simple serum biomarker to elucidate incidence and predictors of advanced fibrosis after LT over a long follow-up period. Methods. We included 440 consecutive patients who underwent LT between 1991 and 2013. Advanced liver fibrosis was defined as FIB-4 > 3.25 beyond 12 months after LT. Results. Over 2030.5 person-years (PY) of follow-up, 189 (43%) developed FIB-4 > 3.25, accounting for an incidence of 9...
2017: Canadian Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28403096/the-efficiency-and-safety-of-fascia-iliaca-block-for-pain-control-after-total-joint-arthroplasty-a-meta-analysis
#15
Peng Zhang, Jifeng Li, Yuze Song, Xiao Wang
BACKGROUND: This meta-analysis aimed to perform a meta-analysis including randomized controlled trials (RCTs) to assess the efficiency and safety of fascia iliaca block (FIB) for pain control in patients undergoing total joint arthroplasty (TJA). METHODS: A systematic search was performed in Medline (1966-2017.03), PubMed (1966-2017.03), Embase (1980-2017.03), ScienceDirect (1985-2017.03) and the Cochrane Library. Study evaluated the efficiency and safety of FIB in TJA was selected...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28397709/ion-damage-free-planarization-or-shallow-angle-sectioning-of-solar-cells-for-mapping-grain-orientation-and-nanoscale-photovoltaic-properties
#16
Yasemin Kutes, Justin Luria, Yu Sun, Andrew Moore, Brandon A Aguirre, Jose L Cruz-Campa, Mark Aindow, David Zubia, Bryan D Huey
Ion beam milling is the most common modern method for preparing specific features for microscopic analysis, even though concomitant ion implantation and amorphization remain persistent challenges, particularly as they often modify materials properties of interest. Atomic force microscopy (AFM), on the other hand, can mechanically mill specific nanoscale regions in plan-view without chemical or high energy ion damage, due to its resolution, directionality, and fine load control. As an example, AFM-nanomilling (AFM-NM) is implemented for top-down planarization of polycrystalline CdTe thin film solar cells, with a resulting decrease in the root mean square (RMS) roughness by an order of magnitude, even better than for a low incidence FIB polished surface...
May 5, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28394122/embedding-free-method-for-preparation-of-cross-sections-of-organic-materials-for-micro-chemical-analysis-using-gas-cluster-ion-beam-sputtering
#17
Ichiro Mihara, Rasmus Havelund, Ian S Gilmore
We present a novel in situ mask method for the preparation of cross-sections of organic materials such as polymer multilayer films suitable for chemical imaging of buried interfaces. We demonstrate this method on a model buried interface system consisting of a piece of Scotch tape adhered to a PET substrate and a protective film used in consumer packaging. A high dose of gallium from a focused ion beam (FIB) was used to produce a damaged overlayer on the surface of the organic sample. The damaged layer has a significantly slower sputter rate compared to the native undamaged organic material...
April 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28387018/transient-elastography-alone-and-in-combination-with-fibrotest-%C3%A2-for-the-diagnosis-of-hepatic-fibrosis-in-alcoholic-liver-disease
#18
Cosmin Sebastian Voican, Alexandre Louvet, Jean-Baptiste Trabut, Micheline Njiké-Nakseu, Sébastien Dharancy, Andrea Sanchez, Marion Corouge, Karima Lamouri, Amandine Lebrun, Axel Balian, Sophie Prévot, Mounia Lachgar, Sophie Maitre, Hélène Agostini, Philippe Mathurin, Gabriel Perlemuter, Sylvie Naveau
BACKGROUND&AIMS: The reliability of transient elastography (TE) to assess liver fibrosis is insufficiently validated in alcoholic liver disease (ALD). We aimed to validate the diagnostic utility of TE for liver fibrosis in patients with excessive alcohol consumption and evaluate whether Fibrotest(®) adds diagnostic value relative to or in combination with TE. METHODS: We conducted a multicenter prospective study on a total of 217 heavy drinkers with high serum aminotransferase levels...
April 7, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28384541/focused-ion-beam-assisted-fabrication-of-soft-high-aspect-ratio-silicon-nanowire-atomic-force-microscopy-probes
#19
Peter Knittel, Nicolas Hibst, Boris Mizaikoff, Steffen Strehle, Christine Kranz
In this study, high-aspect ratio silicon nanowire (SiNW) - modified atomic force microscopy (AFM) probes are fabricated using focused ion beam (FIB) microfabrication technology and vapor-solid-solid synthesis. Commercially available soft silicon nitride probes are used for localized nanowire growth yielding soft high-aspect ratio AFM probes. The SiNW-modified cantilevers are used here for imaging in PeakForce Tappingۛ (PFT) mode, which offers high force control along with valuable information about tip-sample adhesion...
March 28, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28383815/environmental-escherichia-coli-ecology-and-public-health-implications-a-review
#20
REVIEW
Jeonghwan Jang, Hor-Gil Hur, Michael J Sadowsky, Muruleedhara N Byappanahalli, Tao Yan, Satoshi Ishii
Escherichia coli is classified as a rod-shaped, Gram-negative bacterium in the family Enterobacteriaceae. The bacterium mainly inhabits the lower intestinal tract of warm-blooded animals, including humans, and is often discharged into the environment through feces or wastewater effluent. The presence of E. coli in environmental waters has long been considered as an indicator of recent fecal pollution. However, numerous recent studies have reported that some specific strains of E. coli can survive for long periods of time, and potentially reproduce, in extra-intestinal environments...
April 6, 2017: Journal of Applied Microbiology
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