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https://www.readbyqxmd.com/read/29777577/autologous-bone-marrow-mesenchymal-stem-cells-improve-the-quality-and-stability-of-vascularized-flap-surgery-of-irradiated-skin-in-pigs
#1
Christine Linard, Michel Brachet, Carine Strup-Perrot, Bruno L'homme, Elodie Busson, Claire Squiban, Valerie Holler, Michel Bonneau, Jean-Jacques Lataillade, Eric Bey, Marc Benderitter
Cutaneous radiation syndrome has severe long-term health consequences. Because it causes an unpredictable course of inflammatory waves, conventional surgical treatment is ineffective and often leads to a fibronecrotic process. Data about the long-term stability of healed wounds, with neither inflammation nor resumption of fibrosis, are lacking. In this study, we investigated the effect of injections of local autologous bone marrow-derived mesenchymal stromal cells (BM-MSCs), combined with plastic surgery for skin necrosis, in a large-animal model...
May 18, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29773674/deep-sequencing-of-complex-proteoglycans-a-novel-strategy-for-high-coverage-and-site-specific-identification-of-glycosaminoglycan-linked-peptides
#2
Joshua A Klein, Le Meng, Joseph Zaia
Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles.  Expressed in a spatially- and temporally-regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices.  Due to the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods...
May 17, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29762850/effect-of-treating-renal-failure-with-decorin-gene-therapy
#3
R Li, Q-F Wang, W-W Li, X-C Niu
OBJECTIVE: To explore a new approach for treating renal insufficiency with gene therapy by implanting decorin (DCN)-expressing fibroblasts within the renal tissue of rats with renal failure to neutralize TGF-β1 activity. MATERIALS AND METHODS: The 5/6 kidney of the selected male SD rats were removed under aseptic conditions. The rats were grouped randomly after the establishment of the model. There were 10 rats in the sham-operated group (Group A), 10 in the operation control group (without treatment, Group B), 10 in the blank control group [treated with empty vector-transfected fibroblasts (FB (LXSN) cells), Group C], and 10 in the treatment group [treated with FB (LDCNSN) cells, Group D]...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29718376/assessment-of-ablative-fractional-co2-laser-and-er-yag-laser-to-treat-hypertrophic-scars-in-a-red-duroc-pig-model
#4
Luis Rodriguez-Menocal, Stephen S Davis, Sandra Becerra, Marcela Salgado, Joel Gill, Jose Valdes, Ambar Candanedo, Shanmugasundaram Natesan, Michael Solis, Wellington Guzman, Alex Higa, Carl I Schulman, Robert J Christy, Jill Waibel, Evangelos V Badiavas
Hypertrophic scarring is a fibroproliferative process that occurs following a third-degree dermal burn injury, producing significant morbidity due to persistent pain, itching, cosmetic disfigurement, and loss of function due to contractures. Ablative fractional lasers have emerged clinically as a fundamental or standard therapeutic modality for hypertrophic burn scars. Yet the examination of their histopathological and biochemical mechanisms of tissue remodeling and comparison among different laser types has been lacking...
April 28, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/29708036/studies-on-sporadic-non-syndromic-thoracic-aortic-aneurysms-ii-alterations-of-extra-cellular-matrix-components-and-focal-adhesion-proteins
#5
Anna Chiarini, Francesco Onorati, Maddalena Marconi, Alessandra Pasquali, Cristina Patuzzo, Anna Malashicheva, Olga Irtyega, Giuseppe Faggian, Pier F Pignatti, Elisabetta Trabetti, Ubaldo Armato, Ilaria Dal Pra
Background Sporadic non-syndromic thoracic aortic aneurysms (SNSTAAs) are less well understood than familial non-syndromic or syndromic ones. Here, we focused on morphologic and molecular changes of the extracellular matrix of the tunica media of SNSTAAs. Design Single centre design. Methods Surgical media samples from seven SNSTAAs and seven controls underwent quantitative polymerase chain reaction, proteomics-bioinformatics, immunoblotting, histology and immunohistochemistry analysis. Results A down-regulation of Decorin mRNA with unchanged protein levels associated with a remarkable increase of collagen fibres...
June 2018: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/29691233/evaluation-of-biodistribution-of-sulforaphane-after-administration-of-oral-broccoli-sprout-extract-in-melanoma-patients-with-multiple-atypical-nevi
#6
Shawn Tahata, Shivendra V Singh, Yan Lin, Eun-Ryeong Hahm, Jan H Beumer, Susan M Christner, Uma N Rao, Cindy Sander, Ahmad A Tarhini, Hussein Tawbi, Laura K Ferris, Melissa Wilson, Amy Rose, Catherine M Dietz, Ellen K Hughes, Jed W Fahey, Sancy A Leachman, Pamela B Cassidy, Lisa H Butterfield, Hassane M Zarour, John M Kirkwood
Broccoli sprout extract containing sulforaphane (BSE-SFN) has been shown to inhibit ultraviolet radiation-induced damage and tumor progression in skin. This study evaluated the toxicity and potential effects of oral BSE-SFN at three dosages. Seventeen patients who each had at least 2 atypical nevi and a prior history of melanoma were randomly allocated to 50, 100, or 200 µmol oral BSE-SFN daily for 28 days. Atypical nevi were photographed on days 1 and 28, and plasma and nevus samples were taken on days 1, 2, and 28...
April 24, 2018: Cancer Prevention Research
https://www.readbyqxmd.com/read/29670537/impact-of-different-tidal-volume-levels-at-low-mechanical-power-on-ventilator-induced-lung-injury-in-rats
#7
Lillian Moraes, Pedro L Silva, Alessandra Thompson, Cintia L Santos, Raquel S Santos, Marcos V S Fernandes, Marcelo M Morales, Vanessa Martins, Vera L Capelozzi, Marcelo G de Abreu, Paolo Pelosi, Patricia R M Rocco
Tidal volume (VT ) has been considered the main determinant of ventilator-induced lung injury (VILI). Recently, experimental studies have suggested that mechanical power transferred from the ventilator to the lungs is the promoter of VILI. We hypothesized that, as long as mechanical power is kept below a safe threshold, high VT should not be injurious. The present study aimed to investigate the impact of different VT levels and respiratory rates (RR) on lung function, diffuse alveolar damage (DAD), alveolar ultrastructure, and expression of genes related to inflammation [interleukin (IL)-6], alveolar stretch (amphiregulin), epithelial [club cell secretory protein (CC)16] and endothelial [intercellular adhesion molecule (ICAM)-1] cell injury, and extracellular matrix damage [syndecan-1, decorin, and metalloproteinase (MMP)-9] in experimental acute respiratory distress syndrome (ARDS) under low-power mechanical ventilation...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29663371/distribution-of-extracellular-matrix-molecules-in-human-uterine-tubes-during-the-menstrual-cycle-a-histological-and-immunohistochemical-analysis
#8
Carlos Godoy-Guzmán, Claudio Nuñez, Pedro Orihuela, Antonio Campos, Víctor Carriel
The uterine tube (UT) is an important and complex organ of the women's reproductive system. In general, the anatomy and basic histology of this organ are well-known. However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood. The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces. ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues...
April 16, 2018: Journal of Anatomy
https://www.readbyqxmd.com/read/29651716/super-secondary-structure-consisting-of-a-polyproline-ii-helix-and-a-%C3%AE-turn-in-leucine-rich-repeats-in-bacterial-type-iii-secretion-system-effectors
#9
Dashdavaa Batkhishig, Khurelbaatar Bilguun, Purevjav Enkhbayar, Hiroki Miyashita, Robert H Kretsinger, Norio Matsushima
Leucine rich repeats (LRRs) are present in over 100,000 proteins from viruses to eukaryotes. The LRRs are 20-30 residues long and occur in tandem. LRRs form parallel stacks of short β-strands and then assume a super helical arrangement called a solenoid structure. Individual LRRs are separated into highly conserved segment (HCS) with the consensus of LxxLxLxxNxL and variable segment (VS). Eight classes have been recognized. Bacterial LRRs are short and characterized by two prolines in the VS; the consensus is xxLPxLPxx with Nine residues (N-subtype) and xxLPxxLPxx with Ten residues (T-subtype)...
April 12, 2018: Protein Journal
https://www.readbyqxmd.com/read/29644940/transient-scleraxis-overexpression-combined-with-cyclic-strain-enhances-ligamentous-differentiation
#10
Anne C Nichols, Stephen R Werre, Linda A Dahlgren
Efforts to generate tissue engineered anterior cruciate ligament replacements are limited by a lack of methods to derive mature ligament cells. Viral overexpression of the tendon/ligament marker scleraxis (Scx) can drive cell differentiation; however, the use of viral vectors hampers translation to clinical use. In this study, C3H10T1/2 cells were transiently transfected with expression vectors containing the full-length murine Scx cDNA and cultured in 3D collagen hydrogels under static or cyclic strain for up to 14 days...
April 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29620182/response-of-decorin-to-different-intensity-treadmill-running
#11
Shao-Yong Xu, Sheng-Yao Liu, Lei Xu, Song-Yun Deng, Yong-Bin He, Shu-Fen Li, Guo-Xin Ni
Decorin is widely understood to affect collagen fibrillogenesis. However, little is understood about its response to various mechanical loading conditions. In the present study, 36 Wistar rats were randomly divided into control (CON), moderate treadmill running (MTR) and strenuous treadmill running (STR) groups. Animals in the MTR and STR groups were subjected to a 4‑ or 8‑week treadmill running protocol. Subsequently, all Achilles tendons were harvested to perform histological and biochemical analyses...
March 28, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29618652/the-metalloproteinase-proteoglycans-adamts7-and-adamts12-provide-an-innate-tendon-specific-protective-mechanism-against-heterotopic-ossification
#12
Timothy J Mead, Daniel R McCulloch, Jason C Ho, Yaoyao Du, Sheila M Adams, David E Birk, Suneel S Apte
Heterotopic ossification (HO) is a significant clinical problem with incompletely resolved mechanisms. Here, the secreted metalloproteinases ADAMTS7 and ADAMTS12 are shown to comprise a unique proteoglycan class that protects against a tendency toward HO in mouse hindlimb tendons, menisci, and ligaments. Adamts7 and Adamts12 mRNAs were sparsely expressed in murine forelimbs but strongly coexpressed in hindlimb tendons, skeletal muscle, ligaments, and meniscal fibrocartilage. Adamts7-/- Adamts12-/- mice, but not corresponding single-gene mutants, which demonstrated compensatory upregulation of the intact homolog mRNA, developed progressive HO in these tissues after 4 months of age...
April 5, 2018: JCI Insight
https://www.readbyqxmd.com/read/29615673/quantifying-extracellular-matrix-turnover-in-human-lung-scaffold-cultures
#13
Oskar Rosmark, Emma Åhrman, Catharina Müller, Linda Elowsson Rendin, Leif Eriksson, Anders Malmström, Oskar Hallgren, Anna-Karin Larsson-Callerfelt, Gunilla Westergren-Thorsson, Johan Malmström
Remodelling of the extracellular matrix is accomplished by altering the balance between matrix macromolecule production and degradation. However, it is not well understood how cells balance production of new matrix molecules and degradation of existing ones during tissue remodelling and regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic lung fibroblasts cultured with stable isotope labelled amino acids to quantify the balance between matrix production and degradation at a proteome-wide scale...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29605717/biomechanical-and-biomolecular-characterization-of-extracellular-matrix-structures-in-human-colon-carcinomas
#14
Eva Brauchle, Jana Kasper, Ruben Daum, Nicolas Schierbaum, Claudius Falch, Andreas Kirschniak, Tilman E Schäffer, Katja Schenke-Layland
The extracellular matrix (ECM) is extensively remodeled in tumor tissues. Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness. Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown. In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified...
March 29, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29595557/overhanging-dissecting-blebs-immunohistochemical-characterization
#15
Ali S Al-Beshri, Deepak P Edward, Earl Randy Craven
PURPOSE: To determine if glaucoma filtering blebs migrate over or under the cornea epithelium using histopathological and immunohistochemical (IHC) techniques to evaluate the likely origin of the surface epithelium and bleb matrix. METHODS: Histologic and IHC evaluations were performed of normal conjunctiva (n=4,) corneal overhanging-dissecting blebs (n=4,) and leaking blebs over the scleral surface (n=6.) Antibodies were used against epithelial (CK3+12, CK13) and extracellular matrix (ECM) (decorin and keratan sulfate) antigens...
March 27, 2018: Journal of Glaucoma
https://www.readbyqxmd.com/read/29581460/loss-of-stemness-emt-and-supernumerary-tooth-formation-in-cebpb-runx2-murine-incisors
#16
Kazuyuki Saito, Katsu Takahashi, Boyen Huang, Masakazu Asahara, Honoka Kiso, Yumiko Togo, Hiroko Tsukamoto, Sayaka Mishima, Masaki Nagata, Machiko Iida, Yoshihito Tokita, Masato Asai, Akira Shimizu, Toshihisa Komori, Hidemitsu Harada, Mary MacDougall, Manabu Sugai, Kazuhisa Bessho
Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb KO mice demonstrated cementum-like hard tissue in dental pulp, loss of polarity by ameloblasts, enamel matrix in ameloblastic layer, and increased expression of epithelial-mesenchymal transition (EMT) markers in a Cebpb knockdown mouse enamel epithelial stem cell line...
March 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29561836/impaired-proteoglycan-glycosylation-elevated-tgf-%C3%AE-signaling-and-abnormal-osteoblast-differentiation-as-the-basis-for-bone-fragility-in-a-mouse-model-for-gerodermia-osteodysplastica
#17
Wing Lee Chan, Magdalena Steiner, Tomasz Witkos, Johannes Egerer, Björn Busse, Shuji Mizumoto, Jan M Pestka, Haikuo Zhang, Ingrid Hausser, Layal Abo Khayal, Claus-Eric Ott, Mateusz Kolanczyk, Bettina Willie, Thorsten Schinke, Chiara Paganini, Antonio Rossi, Kazuyuki Sugahara, Michael Amling, Petra Knaus, Danny Chan, Martin Lowe, Stefan Mundlos, Uwe Kornak
Gerodermia osteodysplastica (GO) is characterized by skin laxity and early-onset osteoporosis. GORAB, the responsible disease gene, encodes a small Golgi protein of poorly characterized function. To circumvent neonatal lethality of the GorabNull full knockout, Gorab was conditionally inactivated in mesenchymal progenitor cells (Prx1-cre), pre-osteoblasts (Runx2-cre), and late osteoblasts/osteocytes (Dmp1-cre), respectively. While in all three lines a reduction in trabecular bone density was evident, only GorabPrx1 and GorabRunx2 mutants showed dramatically thinned, porous cortical bone and spontaneous fractures...
March 21, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29547593/interleukin-6-promotes-proliferation-but-inhibits-tenogenic-differentiation-via-the-janus-kinase-signal-transducers-and-activators-of-transcription-3-jak-stat3-pathway-in-tendon-derived-stem-cells
#18
Siwei Chen, Ganming Deng, Kaiqun Li, Haonan Zheng, Gang Wang, Bin Yu, Kairui Zhang
BACKGROUND Previous studies demonstrated that tendon-derived stem cells (TDSCs) were vital healing cells and that mRNA expression of anti-inflammatory cytokine IL-6 was significantly upregulated in injured tendons. The aim of the present study was to investigate the effects of IL-6 on the TDSCs in vitro. MATERIAL AND METHODS TDSCs isolated from the Achilles tendons in SD rats were co-cultured with various concentrations of IL-6. Cell proliferation, cell cycle analysis, quantitative real-time PCR, western blotting analysis, and statistical analysis were used in the study...
March 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29521008/biomaterial-scaffolds-as-pre-metastatic-niche-mimics-systemically-alter-the-primary-tumor-and-tumor-microenvironment
#19
Brian A Aguado, Rachel M Hartfield, Grace G Bushnell, Joseph T Decker, Samira M Azarin, Dhaval Nanavati, Matthew J Schipma, Shreyas S Rao, Robert S Oakes, Yining Zhang, Jacqueline S Jeruss, Lonnie D Shea
Primary tumor (PT) immune cells and pre-metastatic niche (PMN) sites are critical to metastasis. Recently, synthetic biomaterial scaffolds used as PMN mimics are shown to capture both immune and metastatic tumor cells. Herein, studies are performed to investigate whether the scaffold-mediated redirection of immune and tumor cells would alter the primary tumor microenvironment (TME). Transcriptomic analysis of PT cells from scaffold-implanted and mock-surgery mice identifies differentially regulated pathways relevant to invasion and metastasis progression...
March 9, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29499356/a-role-for-proteoglycans-in-vascular-disease
#20
REVIEW
Thomas N Wight
The content of proteoglycans (PGs) is low in the extracellular matrix (ECM) of vascular tissue, but increases dramatically in all phases of vascular disease. Early studies demonstrated that glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), keratan sulfate (KS) and heparan sulfate (HS) accumulate in vascular lesions in both humans and in animal models in areas of the vasculature that are susceptible to disease initiation (such as at branch points) and are frequently coincident with lipid deposits...
February 27, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
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