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https://www.readbyqxmd.com/read/28426898/improving-myoblast-differentiation-on-electrospun-poly-%C3%AE%C2%B5-caprolactone-scaffolds
#1
Phammela N Abarzúa-Illanes, Cristina Padilla, Andrea Ramos, Mauricio Isaacs, Jorge Ramos-Grez, Hugo C Olguín, Loreto M Valenzuela
Polymer scaffolds are used as an alternative to support tissue regeneration when it does not occur on its own. Cell response on polymer scaffolds is determined by factors such as polymer composition, topology, and the presence of other molecules. We evaluated the cellular response of murine skeletal muscle myoblasts on aligned or unaligned fibers obtained by electrospinning poly(ε-caprolactone) (PCL), and blends with poly(lactic-co-glycolic acid) (PLGA) or decorin, a proteoglycan known to regulate myogenesis...
April 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28412940/absence-of-the-dermatan-sulfate-chain-of-decorin-does-not-affect-mouse-development
#2
Pierre Moffatt, Yeqing Geng, Lisa Lamplugh, Antonio Nanci, Peter J Roughley
BACKGROUND: In vitro studies suggest that the multiple functions of decorin are related to both its core protein and its dermatan sulfate chain. To determine the contribution of the dermatan sulfate chain to the functional properties of decorin in vivo, a mutant mouse whose decorin lacked a dermatan sulfate chain was generated. RESULTS: Homozygous mice expressing only the decorin core protein developed and grew in a similar manner to wild type mice. In both embryonic and postnatal mice, all connective tissues studied, including cartilage, skin and cornea, appeared to be normal upon histological examination, and their collagen fibrils were of normal diameter and organization...
April 17, 2017: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/28401805/an-engineered-multiphase-3d-microenvironment-to-ensure-the-controlled-delivery-of-cyclic-strain-and-hgdf-5-for-the-tenogenic-commitment-of-hbmscs
#3
Marco Govoni, Anna Berardi, Claudio Muscari, Roberta Campardelli, Francesca Bonafè, Carlo Guarnieri, Ernesto Reverchon, Emanuele Giordano, Nicola Maffulli, Giovanna Della Porta
At present, injuries or rupture of tendons are treated by surgical repair or conservative approaches with unpredictable clinical outcome. Alternative strategies to repair tendon defects without the undesirable side effects associated with the current options are needed. With this in mind, a tissue engineering approach has gained considerable attention as a promising strategy. Here, we investigated a synthetic 3D microenvironment able to interact with stem cells and inducing, via coupled biochemical and physical signals, their early commitment towards the tenogenic lineage...
April 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28390241/decreased-fibrous-encapsulation-and-enhanced-osseointegration-in-vitro-by-decorin-modified-titanium-surface
#4
Ronghan He, Yunxiang Lu, Jianhua Ren, Zhe Wang, Junqi Huang, Lei Zhu, Kun Wang
Orthopedic implants, using materials such as titanium, are extensively used in clinical surgeries. Despite its popularity, titanium is still inadequate to reliable osseointegration due to aseptic loosing. Fibrous encapsulation on the titanium implant interface prevents osseointegration and leads to the loosing of orthopedic implant. In this study, decorin was loaded on titanium surface by polydopamine film to examine fibrous encapsulation inhibition and bone growth acceleration. The coating of decorin was evaluated by X-ray photoelectron spectroscopy (XPS) and fluorescence microscopy...
March 31, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28380688/fabrication-and-characterization-of-a-decellularized-bovine-tendon-sheet-for-tendon-reconstruction
#5
Liang-Ju Ning, Yan-Lin Jiang, Cheng-Hao Zhang, Yi Zhang, Jie-Liang Yang, Jing Cui, Yan-Jing Zhang, Xuan Yao, Jing-Cong Luo, Ting-Wu Qin
Obtaining a performing decellularized tendon scaffold with proper dimensions and adequate availability is highly desirable. However, the combined study of complete decellularization and detailed characterization of native tendon extracellular matrix (ECM) from large animals is still lacking. In the present study, we developed a new decellularization protocol, including physical methods and enzymatic solutions for processing bovine Achilles tendons, and produced a decellularized bovine tendon sheet (DBTS) scaffold for tendon reconstruction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28356486/caveat-emptor-for-researchers-a-rose-will-not-smell-sweet-unless-we-know-its-composition
#6
Josephine C Adams
In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immuno-blotting that certain commercial preparations of the small leucine-rich proteoglycans (SLPRs) decorin and biglycan in fact contained a mix of several proteoglycans that also included fibromodulin and aggrecan. The preparations were thus not suitable to study specific activities of decorin or biglycan...
March 29, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28346368/pathophysiological-significance-of-dermatan-sulfate-proteoglycans-revealed-by-human-genetic-disorders
#7
REVIEW
Shuji Mizumoto, Tomoki Kosho, Shuhei Yamada, Kazuyuki Sugahara
The indispensable roles of dermatan sulfate-proteoglycans (DS-PGs) have been demonstrated in various biological events including construction of the extracellular matrix and cell signaling through interactions with collagen and transforming growth factor-β, respectively. Defects in the core proteins of DS-PGs such as decorin and biglycan cause congenital stromal dystrophy of the cornea, spondyloepimetaphyseal dysplasia, and Meester-Loeys syndrome. Furthermore, mutations in human genes encoding the glycosyltransferases, epimerases, and sulfotransferases responsible for the biosynthesis of DS chains cause connective tissue disorders including Ehlers-Danlos syndrome and spondyloepimetaphyseal dysplasia with joint laxity characterized by skin hyperextensibility, joint hypermobility, and tissue fragility, and by severe skeletal disorders such as kyphoscoliosis, short trunk, dislocation, and joint laxity...
March 27, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/28322869/scaffold-free-high-throughput-generation-of-quiescent-valvular-microtissues
#8
Annelies Roosens, Inès Puype, Ria Cornelissen
AIMS: The major challenge of working with valvular interstitial cells in vitro is the preservation or recovery of their native quiescent state. In this study, a biomimetic approach is used which aims to engineer small volume, high quality valve microtissues, having a potential in regenerative medicine and as a relevant 3D in vitro model to provide insights into valve (patho)biology. METHODS AND RESULTS: To form micro-aggregates, porcine valvular interstitial cells were seeded in agarose micro-wells and cultured in medium supplemented with 250μM Ascorbic Acid 2-phosphate for 22days...
March 18, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28290552/overexpression-of-decorin-promoted-angiogenesis-in-diabetic-cardiomyopathy-via-igf1r-akt-vegf-signaling
#9
Jinsheng Lai, Fuqiong Chen, Jing Chen, Guoran Ruan, Mengying He, Chen Chen, Jiarong Tang, Dao Wen Wang
Microcirculatory dysfunction is believed to play an important role in diabetic cardiomyopathy. The small leucine-rich proteoglycan decorin is generally considered a pro-angiogenic factor. Here, we investigate whether overexpression of decorin ameliorates diabetic cardiomyopathy and its effects on angiogenesis in vivo and in vitro. Diabetes was induced through intraperitoneal injection with streptozotocin combined with a high-fat diet, and decorin was overexpressed via recombinant adeno-associated virus in Wistar rats...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28266029/decorin-loaded-poly-lactic-co-glycolic-acid-nanoparticles-modified-by-anti-alpha-fetoprotein-antibody-preparation-proliferation-inhibition-and-induced-apoptosis-effects-on-hepg2-cells-in-vitro
#10
Qiaoli Yang, Shuyue Wang, Yuan Wang, Yane Qu, Jun Xue, Yang Mi, Yanhong Wang, Xuguang Luo, Zhihua Deng, Guiqin Wang
OBJECTIVES: Decorin (DCN) is a negative regulatory factor for the growth of cancer cells and can inhibit the proliferation, metastasis of cancer cells and angiogenesis in cancer tissues. The aims of this study were to prepare the nanoparticles consisting of DCN and poly lactic-co-glycolic acid (PLGA) modified by anti-alpha fetoprotein (AFP) monoclonal antibody (mAb) and to examine the conventional physical properties, the in-vitro release of DCN and the targeting effect of these nanoparticles on HepG2 cells...
March 7, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28249037/effects-of-a-skin-massaging-device-on-the-ex-vivo-expression-of-human-dermis-proteins-and-in-vivo-facial-wrinkles
#11
Elisa Caberlotto, Laetitia Ruiz, Zane Miller, Mickael Poletti, Lauri Tadlock
Mechanical and geometrical cues influence cell behaviour. At the tissue level, almost all organs exhibit immediate mechanical responsiveness, in particular by increasing their stiffness in direct proportion to an applied mechanical stress. It was recently shown in cultured-cell models, in particular with fibroblasts, that the frequency of the applied stress is a fundamental stimulating parameter. However, the influence of the stimulus frequency at the tissue level has remained elusive. Using a device to deliver an oscillating torque that generates cyclic strain at different frequencies, we studied the effect(s) of mild skin massage in an ex vivo model and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28244027/different-culture-conditions-affect-the-growth-of-human-tendon-stem-progenitor-cells-tspcs-within-a-mixed-tendon-cells-tcs-population
#12
M Viganò, C Perucca Orfei, A Colombini, D Stanco, P Randelli, V Sansone, L de Girolamo
BACKGROUND: Tendon resident cells (TCs) are a mixed population made of terminally differentiated tenocytes and tendon stem/progenitor cells (TSPCs). Since the enrichment of progenitors proportion could enhance the effectiveness of treatments based on these cell populations, the interest on the effect of culture conditions on the TSPCs is growing. In this study the clonal selection and the culture in presence or absence of basic fibroblast growth factor (bFGF) were used to assess their influences on the stemness properties and phenotype specific features of tendon cells...
December 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/28215822/structural-and-functional-analysis-of-two-small-leucine-rich-repeat-proteoglycans-fibromodulin-and-chondroadherin
#13
Patricia Paracuellos, Sebastian Kalamajski, Arkadiusz Bonna, Dominique Bihan, Richard W Farndale, Erhard Hohenester
The small leucine-rich proteoglycans (SLRPs) are important regulators of extracellular matrix assembly and cell signalling. We have determined crystal structures at ~2.2Å resolution of human fibromodulin and chondroadherin, two collagen-binding SLRPs. Their overall fold is similar to that of the prototypical SLRP, decorin, but unlike decorin neither fibromodulin nor chondroadherin forms a stable dimer. A previously identified binding site for integrin α2β1 maps to an α-helix in the C-terminal cap region of chondroadherin...
February 17, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28214837/efficacy-of-glucosamine-sulphate-in-skin-ageing-results-from-an-ex-vivo-anti-ageing-model-and-a-clinical-trial
#14
Audrey Gueniche, Isabelle Castiel-Higounenc
BACKGROUND: Glucosamine sulphate (GS) is essential in the biosynthesis of glycolipids, glycoproteins, glycosaminoglycans (GAGs), hyaluronate, and proteoglycans. Connective tissues primarily contain collagen and proteoglycans and play an important role in skin ageing. OBJECTIVE: The objectives were to assess ex vivo the impact of GS on skin ageing parameters and in vivo the effect of GS on the skin physiology of mature healthy volunteers after oral intake. METHODS: The impact of GS on skin ageing was assessed ex vivo via different immunohistochemical assays and histology and via a clinical study using biopsies...
2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28212278/the-effect-of-the-human-peptide-ghk-on-gene-expression-relevant-to-nervous-system-function-and-cognitive-decline
#15
Loren Pickart, Jessica Michelle Vasquez-Soltero, Anna Margolina
Neurodegeneration, the progressive death of neurons, loss of brain function, and cognitive decline is an increasing problem for senior populations. Its causes are poorly understood and therapies are largely ineffective. Neurons, with high energy and oxygen requirements, are especially vulnerable to detrimental factors, including age-related dysregulation of biochemical pathways caused by altered expression of multiple genes. GHK (glycyl-l-histidyl-l-lysine) is a human copper-binding peptide with biological actions that appear to counter aging-associated diseases and conditions...
February 15, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28199387/dermatan-sulfate-is-a-player-in-the-transglutaminase-2-interaction-network
#16
Grzegorz Wisowski, Ewa M Koźma, Tomasz Bielecki, Adam Pudełko, Krystyna Olczyk
Transglutaminase 2 (TG2) is a multifunctional protein that is primarily engaged in cell adhesion/signaling or shows Ca2+-dependent transglutaminase activity in the extracellular space of tissues. This latter action leads to the cross-linking of the extracellular matrix (ECM) proteins. The enhanced extracellular expression of TG2 is associated with processes such as wound healing, fibrosis or vascular remodeling that are also characterized by a high deposition of dermatan sulfate (DS) proteoglycans in the ECM...
2017: PloS One
https://www.readbyqxmd.com/read/28174297/decorin-inducible-peg3-evokes-beclin-1-mediated-autophagy-and-thrombospondin-1-mediated-angiostasis
#17
Annabel Torres, Maria A Gubbiotti, Renato V Iozzo
We previously discovered that systemic delivery of decorin for treatment of breast carcinoma xenografts induces paternally expressed gene 3 (Peg3), an imprinted gene encoding a zinc finger transcription factor postulated to function as a tumor suppressor. Here we found that de novo expression of Peg3 increased Beclin 1 promoter activity and protein expression. This process required the full-length Peg3 as truncated mutants lacking either the N-terminal SCAN domain or the zinc fingers failed to translocate to the nucleus and promote Beclin 1 transcription...
March 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28138904/the-histopathological-and-pharmacodynamic-effects-of-intradetrusor-decorin-injected-in-a-rabbit-partial-bladder-outlet-obstruction-model
#18
Engin Kaya, Yusuf Kibar, Sercan Yılmaz, Turgay Ebiloglu, Ayhan Ozcan, Melik Seyrek, Oguzhan Yıldız, Kemal Gokhan Ulusoy
PURPOSE: To evaluate whether or not the bladder function can be protected by supporting the detrusor with decorin levels during the fibrotic process. METHODS: Forty-two male rabbits were divided into three main groups, partial bladder outlet obstruction (pBOO) group, pBOO + intradetrusor decorin-injected (IDI) group and control group. Both pBOO and pBOO + IDI groups were divided into three subgroups according to the killing schedule. Histopathological, immunohistochemical and pharmacodynamics studies were performed for the evaluation of fibrotic process and tissue characteristics...
April 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28138416/retinoic-acid-enhances-the-differentiation-of-adipose-derived-stem-cells-to-keratocytes-in-vitro
#19
Amy P Lynch, Mark Ahearne
PURPOSE: All-trans retinoic acid (RA) supplementation was investigated as a method of enhancing the differentiation of human adipose-derived stem cells (ASCs) to corneal keratocytes in vitro, in combination with a chemically defined serum-free medium. METHODS: Adipose-derived stem cells were cultured in monolayer and supplemented with 0.1, 1, or 10 μM RA for 14 days. The effects of RA on cell proliferation, migration, and extracellular matrix (ECM) accumulation were evaluated...
January 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28116125/use-of-fgf-2-and-fgf-18-to-direct-bone-marrow-stromal-stem-cells-to-chondrogenic-and-osteogenic-lineages
#20
Cindy Shu, Susan M Smith, Christopher B Little, James Melrose
AIM: Intervertebral disc degeneration/low back pain is the number one global musculoskeletal condition in terms of disability and socioeconomic impact. MATERIALS & METHODS: Multipotent mesenchymal stem cells (MSCs) were cultured in micromass pellets ± FGF-2 or -18 up to 41 days, matrix components were immunolocalized and gene expression monitored by quantitative-reverse transcription PCR. RESULTS: Chondrogenesis occurred earlier in FGF-18 than FGF-2 cultures...
December 2016: Future Science OA
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