keyword
MENU ▼
Read by QxMD icon Read
search

decorin

keyword
https://www.readbyqxmd.com/read/29346445/transcriptome-analysis-of-skin-fibroblasts-with-dominant-negative-col3a1-mutations-provides-molecular-insights-into-the-etiopathology-of-vascular-ehlers-danlos-syndrome
#1
Nicola Chiarelli, Giulia Carini, Nicoletta Zoppi, Marco Ritelli, Marina Colombi
Vascular Ehlers-Danlos syndrome (vEDS) is a dominantly inherited connective tissue disorder caused by mutations in the COL3A1 gene that encodes type III collagen (COLLIII), which is the major expressed collagen in blood vessels and hollow organs. The majority of disease-causing variants in COL3A1 are glycine substitutions and in-frame splice mutations in the triple helix domain that through a dominant negative effect are associated with the severe clinical spectrum potentially lethal of vEDS, characterized by fragility of soft connective tissues with arterial and organ ruptures...
2018: PloS One
https://www.readbyqxmd.com/read/29340639/biomechanical-rigidity-and-quantitative-proteomics-analysis-of-segmental-regions-of-the-trabecular-meshwork-at-physiologic-and-elevated-pressures
#2
Janice A Vranka, Julia A Staverosky, Ashok P Reddy, Phillip A Wilmarth, Larry L David, Ted S Acott, Paul Russell, Vijay Krishna Raghunathan
Purpose: The extracellular matrix (ECM) of the trabecular meshwork (TM) modulates resistance to aqueous humor outflow, thereby regulating IOP. Glaucoma, a leading cause of irreversible blindness worldwide, is associated with changes in the ECM of the TM. The elastic modulus of glaucomatous TM is larger than age-matched normal TM; however, the biomechanical properties of segmental low (LF) and high flow (HF) TM regions and their response to elevated pressure, are unknown. Methods: We perfused human anterior segments at two pressures using an ex vivo organ culture system...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29311692/surface-functionalization-of-electrospun-scaffolds-using-recombinant-human-decorin-attracts-circulating-endothelial-progenitor-cells
#3
Svenja Hinderer, Katrin Sudrow, Maria Schneider, Monika Holeiter, Shannon Lee Layland, Martina Seifert, Katja Schenke-Layland
Decorin (DCN) is an important small leucine-rich proteoglycan present in the extracellular matrix (ECM) of many organs and tissues. Endothelial progenitor cells (EPCs) are able to interact with the surrounding ECM and bind to molecules such as DCN. Here, we recombinantly produced full-length human DCN under good laboratory practice (GLP) conditions, and after detailed immunological characterization, we investigated its potential to attract murine and human EPCs (mEPCs and hECFCs). Electrospun polymeric scaffolds were coated with DCN or stromal cell-derived factor-1 (SDF-1α) and were then dynamically cultured with both cell types...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29310783/isolation-production-and-analysis-of-small-leucine-rich-proteoglycans-in-bone
#4
David J Kirby, Marian F Young
Small leucine-rich proteoglycans (SLRPs) are a unique class of proteins that exist in the extracellular matrix, playing key roles in cell proliferation and function. In bone, SLRPs such as biglycan and decorin affect osteogenesis and bone remodeling. Their essential role in this organ system has created the need to isolate these proteins for study. Bone presents unique obstacles to the study of proteins; however, through the use of demineralizing agents, efficient methods of the purification of proteoglycans have been developed...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29296764/antiangiogenic-effects-of-decorin-restored-by-unfractionated-low-molecular-weight-and-nonanticoagulant-heparins
#5
Amy K L Chui, Tilini N Gunatillake, Vera Ignjatovic, Paul T Monagle, Padma Murthi, Shaun P Brennecke, John M Whitelock, Joanne M Said
Pregnancies affected by preeclampsia (PE) or fetal growth restriction (FGR) display increases in thrombin generation and reductions in angiogenesis and cell growth. There is significant interest in the potential for low molecular weight heparins (LMWHs) to reduce the recurrence of PE and FGR. However, LMWH is associated with an increased risk of bleeding. Therefore, it is of vital importance to determine the exact molecular function of heparins in pregnancy if they are used as therapy for pregnant women. We aimed to determine this using our model for PE/FGR in microvascular endothelial cells...
July 11, 2017: Blood Advances
https://www.readbyqxmd.com/read/29290137/small-leucine-rich-proteoglycans-in-renal-inflammation-two-sides-of-the-coin
#6
Madalina V Nastase, Andrea Janicova, Heiko Roedig, Louise Tzung-Harn Hsieh, Malgorzata Wygrecka, Liliana Schaefer
It is now well-established that members of the small leucine-rich proteoglycan (SLRP) family act in their soluble form, released proteolytically from the extracellular matrix (ECM), as danger-associated molecular patterns (DAMPs). By interacting with Toll-like receptors (TLRs) and the inflammasome, the two SLRPs, biglycan and decorin, autonomously trigger sterile inflammation. Recent data indicate that these SLRPs, besides their conventional role as pro-inflammatory DAMPs, additionally trigger anti-inflammatory signaling pathways to tightly control inflammation...
October 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29229446/clinical-utility-of-decorin-in-follicular-fluid-as-a-biomarker-of-oocyte-potential
#7
Yuki Sawada, Takeshi Sato, Chieko Saito, Fumiko Ozawa, Yasuhiko Ozaki, Mayumi Sugiura-Ogasawara
This study investigated the concentration of decorin (DCN) in mature follicular fluid and the existence in the granulosa cells. It also investigated whether DCN is useful as a biomarker for outcomes of assisted reproductive technology (ART). A retrospective cohort study was performed involving 130 oocytes of 88 patients treated with ART because of unexplained infertility. The concentration of DCN in the follicular fluid (F-DCN) was 39.26ng/ml (median value); it was higher than that in serum. F-DCN of the oocytes fertilized by intracytoplasmic sperm injection (ICSI) was significantly lower than that of oocytes that were not fertilized (33...
December 8, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29214350/co-culture-of-human-fibroblasts-and-borrelia-burgdorferi-enhances-collagen-and-growth-factor-mrna
#8
Elisabeth Aberer, Milana Surtov-Pudar, Daniel Wilfinger, Alexander Deutsch, Gerd Leitinger, Helmut Schaider
Skin fibrosis has been reported in Borrelia burgdorferi infection in Europe, but has been questioned by several authors. The objective of the present study was to examine the interaction of skin fibroblasts with B. burgdorferi sensu stricto B31 (BB) and B. afzelii (BA) in vitro by electron microscopy. We also determined the expression of collagen type I, TGF-β, FGF-1, calreticulin (CALR), decorin (DCN), and PDGF-α at the mRNA level in Borrelia/fibroblast co-cultures. Intact Borrelia attach to and transmigrate fibroblasts, and undergo cystic transformation outside the fibroblasts...
December 6, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/29210090/towards-understanding-the-dose-and-timing-effect-of-hydrocortisone-treatment-on-the-scleral-ossicle-system-within-the-chicken-eye
#9
Christine L Hammer, Tamara A Franz-Odendaal
Previous work, almost four decades ago, showed that hydrocortisone (HC) treatment reduces the number of skeletogenic condensations that give rise to the scleral ossicles in the chicken eye. The scleral ossicles are a ring of overlapping intramembranous bones, the sclerotic ring, and are present in most reptiles, including birds. The scleral condensations that give rise to the scleral ossicles are induced by a series of overlying thickenings (or papillae) of the conjunctival epithelium. Here, we further explore the effects of altering the dosage and timing of HC treatment on the morphology and number of skeletogenic condensations and conjunctival papillae...
December 6, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/29200094/characteristics-of-a-national-level-female-weightlifter-peaking-for-competition-a-case-study
#10
Caleb D Bazyler, Satoshi Mizuguchi, Michael C Zourdos, Kimitake Sato, Ashley A Kavanaugh, Brad H DeWeese, Kevin F Breuel, Michael H Stone
This study investigated physiological and performance changes of a national level 69kg female weightlifter following three competition phases over a 28-week training period. The athlete first trained for a regional championship (weeks 1-12), followed by a local competition (weeks 13-23) and the national championship (weeks 24-28). Body mass, vastus lateralis cross-sectional area, and unloaded and loaded squat jump performance were assessed weekly during each 4-week competition phase. Serum biomarkers, and dynamic mid-thigh pulls were assessed prior to and after each competition phase...
December 1, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29193831/mimicking-corneal-stroma-using-keratocyte-loaded-photopolymerizable-methacrylated-gelatin-hydrogels
#11
Cemile Kilic Bektas, Vasif Hasirci
Cell-laden GelMA hydrogels with high (ca. 90%) transparency were prepared to mimic the natural form and function of corneal stroma. They were synthesized from methacrylated gelatin with a methacrylation degree of 70% as determined by NMR. Hydrogels were strong enough to withstand handling. Stability studies showed that 87% of the GelMA hydrogels remained after 21 days in PBS. Cell viability in the first two days was over 90% for the human keratocytes loaded in the gels as determined with the Live-Dead analysis...
November 29, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29158532/recombinant-decorin-fusion-protein-attenuates-murine-abdominal-aortic-aneurysm-formation-and-rupture
#12
Yue Shen, Valerio Russo, Matthew R Zeglinski, Stephanie L Sellers, Zhengguo Wu, Cameron Oram, Stephanie Santacruz, Yulia Merkulova, Christopher Turner, Keerit Tauh, Hongyan Zhao, Tatjana Bozin, Lubos Bohunek, Haishan Zeng, Michael A Seidman, R Chris Bleackley, Bruce M McManus, Erkki Ruoslahti, Tero A H Järvinen, David J Granville
Decorin (DCN) is a small-leucine rich proteoglycan that mediates collagen fibrillogenesis, organization, and tensile strength. Adventitial DCN is reduced in abdominal aortic aneurysm (AAA) resulting in vessel wall instability thereby predisposing the vessel to rupture. Recombinant DCN fusion protein CAR-DCN was engineered with an extended C-terminus comprised of CAR homing peptide that recognizes inflamed blood vessels and penetrates deep into the vessel wall. In the present study, the role of systemically-administered CAR-DCN in AAA progression and rupture was assessed in a murine model...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29156766/isobaric-tags-for-relative-and-absolute-quantification-based-proteomic-analysis-of-testis-biopsies-in-rhesus-monkeys-treated-with-transient-scrotal-hyperthermia
#13
Meng Rao, Sha Ma, Shifu Hu, Hui Lei, Yanqing Wu, Yanfei Zhou, Wei Xia, Changhong Zhu
This study aimed to examine the cellular and molecular events that occur in rhesus monkey testes after scrotal hyperthermia. Eight male adult rhesus monkeys were subjected to scrotal hyperthermia at 43°C for 30 min daily for 6 consecutive days. Sperm concentration, reproductive hormones, and testis histology were examined before hyperthermia (day 0), and at 8, 15, 30, 45, 60, 75, and 90 days after the initiation of hyperthermia. iTRAQ-based proteomic analysis was conducted on testicular tissues collected on days 0, 8, and 60 to identify differentially expressed proteins at the early and recovery stages of testicular damage...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29143549/negative-impact-of-hyperglycaemia-on-mouse-alveolar-development
#14
Mei-Yao He, Guang Wang, Sha-Sha Han, Ke Li, Ya Jin, Meng Liu, Zhen-Peng Si, Ju Wang, Guo-Sheng Liu, Xuesong Yang
Diabetes mellitus in pregnancy has been known to affect the embryonic development of various systems, including cardiovascular and nervous systems. However, whether this disease could have a negative impact on embryonic respiratory system remains controversial. In this study, we demonstrated that pregestational diabetes mellitus (PGDM)-induced defects in lung development in mice are mainly characterized by the changes in the morphological structure of the lung. Immunostaining and Western blotting showed that proliferation increased and apoptosis decreased in PGDM...
November 16, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29138241/decorin-a-growth-hormone-regulated-protein-in-humans
#15
Neha Bahl, Glenn Stone, Mark McLean, Ken K Y Ho, Vita Birzniece
CONTEXT: Growth hormone (GH) stimulates connective tissue and muscle growth, an effect that is potentiated by testosterone. Decorin, a myokine and a connective tissue protein, stimulates connective tissue accretion and muscle hypertrophy. Whether GH and testosterone regulate decorin in humans is not known. OBJECTIVE: To determine whether decorin is stimulated by GH and testosterone. DESIGN: Randomized, placebo-controlled, double-blind study...
November 14, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/29121102/histopathologic-and-immunohistochemical-features-of-capsular-tissue-around-failed-ahmed-glaucoma-valves
#16
Alka Mahale, Fatma Fikri, Khitam Al Hati, Sami Al Shahwan, Ibrahim Al Jadaan, Hind Al Katan, Rajiv Khandekar, Azza Maktabi, Deepak P Edward
Impervious encapsulation around Ahmed glaucoma valve (AGV) results in surgical failure raising intraocular pressure (IOP). Dysregulation of extracellular matrix (ECM) molecules and cellular factors might contribute to increased hydraulic resistance to aqueous drainage. Therefore, we examined these molecules in failed AGV capsular tissue. Immunostaining for ECM molecules (collagen I, collagen III, decorin, lumican, chondroitin sulfate, aggrecan and keratan sulfate) and cellular factors (αSMA and TGFβ) was performed on excised capsules from failed AGVs and control tenon's tissue...
2017: PloS One
https://www.readbyqxmd.com/read/29111696/sequencing-the-dermatan-sulfate-chain-of-decorin
#17
Yanlei Yu, Jiana Duan, Franklin E Leach, Toshihiko Toida, Kyohei Higashi, Hong Zhang, Fuming Zhang, I Jonathan Amster, Robert J Linhardt
Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis. One of the most structurally complex classes of glycoconjugates is the proteoglycans (PGs) and their glycosaminoglycan (GAG) side chains. Previously, our laboratory solved the structure of the chondroitin sulfate chain of the bikunin PG. The current study examines the much more complex structure of the dermatan sulfate GAG chain of decorin PG...
November 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29100340/adenovirus-mediated-decorin-expression-induces-cancer-cell-death-through-activation-of-p53-and-mitochondrial-apoptosis
#18
A-Rum Yoon, JinWoo Hong, Chae-Ok Yun
Decorin (DCN) is a small leucine-rich proteoglycan that plays an important role in the regulation of apoptosis, proliferation, intercellular contact, and cell migration. Here we have investigated the detailed mechanism of apoptotic cell death induced by DCN expression. A marked increase in cytotoxicity was observed for both DCN-expressing replication-incompetent (dE1/DCN) and -competent (dB/DCN) adenoviruses (Ads) compared to the corresponding control Ads. FACS and TUNEL assays revealed that the expression of DCN induced apoptotic cell death...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29080840/decorin-is-a-devouring-proteoglycan-remodeling-of-intracellular-catabolism-via-autophagy-and-mitophagy
#19
REVIEW
Simone Buraschi, Thomas Neill, Renato V Iozzo
Autophagy, a fundamental and evolutionarily-conserved eukaryotic pathway, coordinates a complex balancing act for achieving both nutrient and energetic requirements for proper cellular function and homeostasis. We have discovered that soluble proteoglycans evoke autophagy in endothelial cells and mitophagy in breast carcinoma cells by directly interacting with receptor tyrosine kinases, including VEGF receptor 2 and Met. Under these circumstances, autophagic regulation is considered "non-canonical" and is epitomized by the bioactivity of the small leucine-rich proteoglycan, decorin...
November 7, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29072564/the-histopathological-effects-of-intracavernosal-mitomycin-c-injection-in-a-rat-peyronie-s-disease-model
#20
Engin Kaya, Yusuf Kibar, Sercan Yilmaz, Ayhan Ozcan, Burak Kopru, Turgay Ebiloglu, Hasan Cem Irkılata
INTRODUCTION: We aimed to evaluate whether or not nitomycin-C (MMC) has an anti-fibrotic effect on transforming growth factor beta (TGF-β)-induced Peyronie's disease (PD) in a rat model. METHODS: Eighteen 12-week-old male Sprague-Dawley rats were divided into three groups: Group 1= TGF-β1 (n:7); Group 2= TGF-β1+MMC (n:7); and Group 3= Sham group (0.25 ml bovine serum albumin injected) (n:4). All groups were sacrificed on the sixth week of the procedure and their penises were excised...
November 11, 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
keyword
keyword
91973
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"