keyword
MENU ▼
Read by QxMD icon Read
search

Multivesicular

keyword
https://www.readbyqxmd.com/read/27924850/nmr-studies-on-the-interactions-between-yeast-vta1-and-did2-during-the-multivesicular-bodies-sorting-pathway
#1
Jie Shen, Zhongzheng Yang, Jiaolong Wang, Bin Zhao, Wenxian Lan, Chunxi Wang, Xu Zhang, Cody J Wild, Maili Liu, Zhaohui Xu, Chunyang Cao
As an AAA-ATPase, Vps4 is important for function of multivesicular bodies (MVB) sorting pathway, which involves in cellular phenomena ranging from receptor down-regulation to viral budding to cytokinesis. The activity of Vps4 is stimulated by the interactions between Vta1 N-terminus (named as Vta1NTD) and Did2 fragment (176-204 aa) (termed as Did2176-204) or Vps60 (128-186 aa) (termed as Vps60128-186). The structural basis of how Vta1NTD binds to Did2176-204 is still unclear. To address this, in this report, the structure of Did2176-204 in complex with Vta1NTD was determined by NMR techniques, demonstrating that Did2176-204 interacts with Vta1NTD through its helix α6' extending over the 2(nd) and the 3(rd) α-helices of Vta1NTD microtubule interacting and transport 1 (MIT1) domain...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27913999/the-role-of-her2-egfr-and-other-receptor-tyrosine-kinases-in-breast-cancer
#2
Jennifer L Hsu, Mien-Chie Hung
Breast cancer affects approximately 1 in 8 women, and it is estimated that over 246,660 women in the USA will be diagnosed with breast cancer in 2016. Breast cancer mortality has decline over the last two decades due to early detection and improved treatment. Over the last few years, there is mounting evidence to demonstrate the prominent role of receptor tyrosine kinases (RTKs) in tumor initiation and progression, and targeted therapies against the RTKs have been developed, evaluated in clinical trials, and approved for many cancer types, including breast cancer...
December 2, 2016: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/27908272/exosome-encapsulated-micrornas-as-potential-circulating-biomarkers-in-colon-cancer
#3
Mina Hosseini, Sara Khatamianfar, Seyed Mahdi Hassanian, Reza Nedaeinia, Mojtaba Shafiee, Mina Maftouh, Majid Ghayour-Mobarhan, Soudabeh Shahid Sales, Amir Avan
Exosome-encapsulated microRNAs are being suggested as a new class novel biomarker and diagnostic and predictive markers in colorectal cancer. These particles are released from many cell types into the extracellular space upon fusion of multivesicular bodies (MVB) with the plasma membrane. They contain a wide variety of information, including proteins, lipids, RNAs, non-transcribed RNAs, microRNAs, which can be circulated in various body fluids (e.g., blood, salvia, ascites, urine). Exosomes can be taken up by neighboring or distant cells and thereby modulate the functional of recipient cells and play a key role in disease progression or facilitate metastasis in cancers...
December 1, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27900319/spleen-dependent-immune-protection-elicited-by-cpg-adjuvanted-reticulocyte-derived-exosomes-from-malaria-infection-is-associated-with-changes-in-t-cell-subsets-distribution
#4
Lorena Martín-Jaular, Armando de Menezes-Neto, Marta Monguió-Tortajada, Aleix Elizalde-Torrent, Míriam Díaz-Varela, Carmen Fernández-Becerra, Francesc E Borras, Maria Montoya, Hernando A Del Portillo
Reticulocyte-derived exosomes (rex) are 30-100 nm membrane vesicles of endocytic origin released during the maturation of reticulocytes to erythrocytes upon fusion of multivesicular bodies with the plasma membrane. Combination of CpG-ODN with rex obtained from BALB/c mice infected with the reticulocyte-prone non-lethal P. yoelii 17X malaria strain (rexPy), had been shown to induce survival and long lasting protection. Here, we show that splenectomized mice are not protected upon rexPy+CpG inmunizations and that protection is restored upon passive transfer of splenocytes obtained from animals immunized with rexPy+CpG...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27895152/spatio-temporal-uncoupling-of-mirna-mediated-translational-repression-and-target-rna-degradation-controls-mirnp-recycling-in-mammalian-cells
#5
Mainak Bose, Bahnisikha Barman, Avijit Goswami, Suvendra N Bhattacharyya
miRNA-mediated repression control expression of more than half of protein coding genes in metazoan animals. Translation repression is associated with target mRNA degradation initiated by decapping and deadenylation of the repressed mRNAs. Earlier evidence suggest Endoplasmic Reticulum (ER) as the site where miRNPs interact with their targets before the translation repression sets in but the subcellular location of subsequent degradation of miRNA-repressed messages is largely unidentified. Here, we explore the subcellular distribution of essential components of degradation machineries of miRNA-targeted mRNAs...
November 28, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27882925/isgylation-controls-exosome-secretion-by-promoting-lysosomal-degradation-of-mvb-proteins
#6
Carolina Villarroya-Beltri, Francesc Baixauli, María Mittelbrunn, Irene Fernández-Delgado, Daniel Torralba, Olga Moreno-Gonzalo, Sara Baldanta, Carlos Enrich, Susana Guerra, Francisco Sánchez-Madrid
Exosomes are vesicles secreted to the extracellular environment through fusion with the plasma membrane of specific endosomes called multivesicular bodies (MVB) and mediate cell-to-cell communication in many biological processes. Posttranslational modifications are involved in the sorting of specific proteins into exosomes. Here we identify ISGylation as a ubiquitin-like modification that controls exosome release. ISGylation induction decreases MVB numbers and impairs exosome secretion. Using ISG15-knockout mice and mice expressing the enzymatically inactive form of the de-ISGylase USP18, we demonstrate in vitro and in vivo that ISG15 conjugation regulates exosome secretion...
November 24, 2016: Nature Communications
https://www.readbyqxmd.com/read/27879451/tetraspanins-and-mouse-oocyte-microvilli-related-to-fertilizing-ability
#7
Achraf Benammar, Ahmed Ziyyat, Brigitte Lefèvre, Jean-Philippe Wolf
Our electron microscopy observations demonstrate for the first time that the number of microvilli on the mice oocyte membrane decreases when meiosis progresses from prophase I to metaphase II (MII) stage, and the morphology of the microvilli also changes. Microvilli are significantly shorter and larger on the ovulated oocyte membrane than at the previous stages. Although clathrin vesicles clearly disappear during oocyte maturation, exosome-like vesicles begin to be secreted at the metaphase I stage, more strongly at the MII stage...
November 21, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27879389/vps36-dependent-multivesicular-bodies-are-critical-for-plasmamembrane-protein-turnover-and-vacuolar-biogenesis
#8
Huei-Jing Wang Wang, Ya-Wen Hsu, Cian-Ling Guo, Wann-Nang Jane, Hao Wang, Liwen Jiang, Guang-Yuh Jauh
Most eukaryotic cells target ubiquitinated plasma membrane proteins for vacuolar degradation in response to environmental and developmental cues. This process involves endosomal sorting complexes required for transport (ESCRT). However, little is known about the cellular mechanisms of ESCRTs in plants. Here, we studied the function of one ESCRT-II component, VPS36, which shows ubiquitin-binding activity and may form a putative ESCRT-II with VPS22 and VPS25 in Arabidopsis. Recessive mutation of the ubiquitously expressed VPS36 causes multiple defects, including delayed embryogenesis, defective root elongation and limited expansion of cotyledons, and these effects can be complemented by its genomic DNA...
November 22, 2016: Plant Physiology
https://www.readbyqxmd.com/read/27875100/the-small-gtpase-ral-orchestrates-mvb-biogenesis-and-exosome-secretion
#9
Vincent Hyenne, Michel Labouesse, Jacky G Goetz
Extracellular vesicles are novel mediators of cell-cell communication. They are present in all species and involved in physiological and pathological processes. One class of extracellular vesicles, the exosomes, originate from an endosomal compartment, the MultiVesicular Body (MVB), and are released from the cell upon fusion of the MVB with the plasma membrane. Although different molecular mechanisms have been associated with MVB biogenesis and exosome secretion, how they coordinate remains poorly documented...
November 22, 2016: Small GTPases
https://www.readbyqxmd.com/read/27849413/storage-pool-diseases-illuminate-platelet-dense-granule-biogenesis
#10
Andrea L Ambrosio, Santiago M Di Pietro
Platelet dense granules (DGs) are membrane bound compartments that store polyphosphate and small molecules such as ADP, ATP, Ca(2+), and serotonin. The release of DG contents plays a central role in platelet aggregation to form a hemostatic plug. Accordingly, congenital deficiencies in the biogenesis of platelet DGs underlie human genetic disorders that cause storage pool disease and manifest with prolonged bleeding. DGs belong to a family of lysosome-related organelles, which also includes melanosomes, the compartments where the melanin pigments are synthesized...
November 16, 2016: Platelets
https://www.readbyqxmd.com/read/27848060/man1-restricts-bmp-signaling-during-synaptic-growth-in-drosophila
#11
Ulrike Laugks, Marie Hieke, Nicole Wagner
Bone morphogenic protein (BMP) signaling is crucial for coordinated synaptic growth and plasticity. Here, we show that the nuclear LEM-domain protein MAN1 is a negative regulator of synaptic growth at Drosophila larval and adult neuromuscular junctions (NMJs). Loss of MAN1 is associated with synaptic structural defects, including floating T-bars, membrane attachment defects, and accumulation of vesicles between perisynaptic membranes and membranes of the subsynaptic reticulum. In addition, MAN1 mutants accumulate more heterogeneously sized vesicles and multivesicular bodies in larval and adult synapses, the latter indicating that MAN1 may function in synaptic vesicle recycling and endosome-to-lysosome trafficking...
November 15, 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/27810927/scramblase-tmem16f-terminates-t-cell-receptor-signaling-to-restrict-t-cell-exhaustion
#12
Yu Hu, Ji Hyung Kim, Kangmin He, Qi Wan, Jessica Kim, Melanie Flach, Tom Kirchhausen, Andrea Vortkamp, Florian Winau
In chronic infection, T cells become hyporesponsive to antigenic stimulation to prevent immunopathology. Here, we show that TMEM16F is required to curb excessive T cell responses in chronic infection with virus. TMEM16F-deficient T cells are hyperactivated during the early phase of infection, exhibiting increased proliferation and cytokine production. Interestingly, this overactivation ultimately leads to severe T cell exhaustion and the inability of the host to control viral burden. Mechanistically, we identify TMEM16F as the dominant lipid scramblase in T lymphocytes that transports phospholipids across membranes...
November 14, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27805610/applications-of-phluorin-for-quantitative-kinetic-and-high-throughput-analysis-of-endocytosis-in-budding-yeast
#13
Derek C Prosser, Kristie Wrasman, Thaddeus K Woodard, Allyson F O'Donnell, Beverly Wendland
Green fluorescent protein (GFP) and its variants are widely used tools for studying protein localization and dynamics of events such as cytoskeletal remodeling and vesicular trafficking in living cells. Quantitative methodologies using chimeric GFP fusions have been developed for many applications; however, GFP is somewhat resistant to proteolysis, thus its fluorescence persists in the lysosome/vacuole, which can impede quantification of cargo trafficking in the endocytic pathway. An alternative method for quantifying endocytosis and post-endocytic trafficking events makes use of superecliptic pHluorin, a pH-sensitive variant of GFP that is quenched in acidic environments...
October 23, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27802428/effect-of-melatonin-on-telocytes-in-the-seminal-vesicle-of-the-soay-ram-an-immunohistochemical-ultrastructural-and-morphometrical-study
#14
Hanan H Abd-Elhafeez, Doaa M Mokhtar, Ahmed H S Hassan
Telocytes (TCs) are a special type of interstitial cell with characteristic cellular processes that are described in many organs. The current study aimed to investigate TCs in seminal vesicles of the Soay ram responding to melatonin treatment during the nonbreeding season by conventional immunohistochemical stains, and to detect the ultrastructural and morphometrical changes of TCs. TCs in the control group showed a broad range of staining affinity and also reacted positively to CD117/c-kit, CD34, desmin, S-100 protein, and progesterone and estrogen receptors alpha, while after melatonin treatment a strong reaction against these 6 antibodies was recorded...
November 2, 2016: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/27783487/hollow-superparamagnetic-microballoons-from-lifelike-self-directed-pickering-emulsions-based-on-patchy-nanoparticles
#15
Tim Granath, Angela Sanchez-Sanchez, Aleksey Shmeliov, Valeria Nicolosi, Vanessa Fierro, Alain Celzard, Karl Mandel
Herein, the formation of hollow microballoons derived from superparamagnetic iron oxide nanoparticles with silica patches is reported. Depending on the experimental conditions, single- or multishelled superparamagnetic microballoons as well as multivesicular structures were obtained. We show how such structural changes follow a lifelike process that is based on self-directing Pickering emulsions. We further demonstrate that the key toward the formation of such complex architectures is the patchy nature of the nanoparticles...
October 31, 2016: ACS Nano
https://www.readbyqxmd.com/read/27768850/probing-the-heterogeneity-of-protein-kinase-activation-in-cells-by-super-resolution-microscopy
#16
Ruobing Zhang, Gilbert O Fruhwirth, Oana Coban, James E Barrett, Thomas Burgoyne, Sang Hak Lee, Paul Dennis Simonson, Murat Baday, Boris Kholodenko, Clare E Futter, Tony Ng, Paul R Selvin
Heterogeneity of mitogen-activated protein kinase (MAPK) activation in genetically identical cells, which occurs in response to epidermal growth factor receptor (EGFR) signaling, remains poorly understood. MAPK cascades integrate signals emanating from different EGFR spatial locations, including the plasma membrane and endocytic compartment. We previously hypothesized that in EGF-stimulated cells the MAPK phosphorylation (pMAPK) level and activity are largely determined by the spatial organization of the EGFR clusters within the cell...
October 21, 2016: ACS Nano
https://www.readbyqxmd.com/read/27765817/adaptor-protein-cd2ap-and-l-type-lectin-lman2-regulate-exosome-cargo-protein-trafficking-through-the-golgi-complex
#17
Sang-Ho Kwon, Sekyung Oh, Marisa Nacke, Keith E Mostov, Joshua H Lipschutz
Exosomes, 40-150-nm extracellular vesicles, transport biological macromolecules that mediate intercellular communications. Although exosomes are known to originate from maturation of endosomes into multivesicular endosomes (also known as multivesicular bodies) with subsequent fusion of the multivesicular endosomes with the plasma membrane, it remains unclear how cargos are selected for exosomal release. Using an inducible expression system for the exosome cargo protein GPRC5B and following its trafficking trajectory, we show here that newly synthesized GPRC5B protein accumulates in the Golgi complex prior to its release into exosomes...
December 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27742708/a-new-molecular-link-between-defective-autophagy-and-erythroid-abnormalities-in-chorea-acanthocytosis
#18
Francesca Lupo, Elena Tibaldi, Alessandro Matte, Alok K Sharma, Anna Maria Brunati, Seth L Alper, Carlo Zancanaro, Donatella Benati, Angela Siciliano, Mariarita Bertoldi, Francesca Zonti, Alexander Storch, Ruth H Walker, Adrian Danek, Benedikt Bader, Andreas Hermann, Lucia De Franceschi
Chorea-acanthocytosis is one of the hereditary neurodegenerative disorders known as the neuroacanthocytoses. Chorea-acanthocytosis is characterized by circulating acanthocytes deficient in chorein, a protein of unknown function. We report here for the first time that chorea-acanthocytosis red-cells are characterized by impaired autophagy, with cytoplasmic accumulation of active Lyn and of autophagy-related proteins Ulk1, Atg7. In chorea-acanthocytosis erythrocytes, active Lyn is sequestered by HSP90-70 to form high-molecular-weight complexes that stabilize and protect Lyn from its proteasomal degradation, contributing to toxic Lyn accumulation...
October 14, 2016: Blood
https://www.readbyqxmd.com/read/27725157/multivesicular-body-formation-enhancement-and-exosome-release-during-endoplasmic-reticulum-stress
#19
Soshi Kanemoto, Ryota Nitani, Tatsuhiko Murakami, Masayuki Kaneko, Rie Asada, Koji Matsuhisa, Atsushi Saito, Kazunori Imaizumi
The endoplasmic reticulum (ER) plays a pivotal role in maintaining cellular homeostasis. However, numerous environmental and genetic factors give rise to ER stress by inducing an accumulation of unfolded proteins. Under ER stress conditions, cells initiate the unfolded protein response (UPR). Here, we demonstrate a novel aspect of the UPR by electron microscopy and immunostaining analyses, whereby multivesicular body (MVB) formation was enhanced after ER stress. This MVB formation was influenced by inhibition of ER stress transducers inositol required enzyme 1 (IRE1) and PKR-like ER kinase (PERK)...
October 7, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27707921/the-intracellular-cholesterol-transport-inhibitor-u18666a-inhibits-the-exosome-dependent-release-of-mature-hepatitis-c-virus
#20
Fabian Elgner, Huimei Ren, Regina Medvedev, Daniela Ploen, Kiyoshi Himmelsbach, Klaus Boller, Eberhard Hildt
: Hepatitis C virus (HCV) particles are described as lipoviroparticles which are released similarly to very-low-density lipoproteins (VLDLs). However, the release mechanism is still poorly understood; the canonical endoplasmic reticulum-Golgi intermediate compartment (ERGIC) pathway as well as endosome-dependent release has been proposed. Recently, the role of exosomes in the transmission of HCV has been reported. Only a minor fraction of the de novo-synthesized lipoviroparticles is released by the infected cell...
December 15, 2016: Journal of Virology
keyword
keyword
91940
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"