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Multiplex and cytokine

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https://www.readbyqxmd.com/read/28226398/dysregulation-of-interleukin-5-expression-in-familial-eosinophilia
#1
Senbagavalli Prakash Babu, Yun-Yun K Chen, Sandra Bonne-Annee, Jun Yang, Irina Maric, Timothy G Myers, Thomas B Nutman, Amy D Klion
BACKGROUND: Familial eosinophilia (FE) is a rare autosomal dominant inherited disorder characterized by the presence of lifelong peripheral eosinophilia (>1500/μL). Mapped to chromosome 5q31-q33, the genetic cause of FE is unknown, and prior studies have failed to demonstrate a primary abnormality in the eosinophil lineage. OBJECTIVE: The aim of the present study was to identify the cells driving the eosinophilia in FE. METHODS: Microarray analysis and real-time PCR were used to examine transcriptional differences in peripheral blood mononuclear cells (PBMC), and in purified cell subsets from affected and unaffected family members belonging to a single large kindred...
February 22, 2017: Allergy
https://www.readbyqxmd.com/read/28225337/multiple-cytokines-for-the-detection-of-mycobacterium-tuberculosis-infection-in-children-with-tuberculosis
#2
N Nausch, C Lundtoft, G Schulz, H Henckel, E Mayatepek, B Fleischer, F M Marx, M Jacobsen
SETTING: Interferon-gamma (IFN-γ) release assays (IGRAs) play an important role in the diagnosis of Mycobacterium tuberculosis infection. However, in children with tuberculosis (TB), some studies have shown increased frequencies of false-negative or indeterminate IGRA results. OBJECTIVE: To analyse the spectrum of different cytokines to improve the diagnostic accuracy of IGRAs in latent tuberculous infection (LTBI) and active TB. DESIGN: We performed multiplex cytokine expression analysis of QuantiFERON® Gold In-Tube supernatants in children with active TB (n = 21) and disease-free contacts with (n = 15) and without LTBI (n = 12), to determine the sensitivity and specificity of the modified tests...
March 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28225209/association-of-the-il-10-receptor-a536g-s138g-loss-of-function-variant-with-multiple-sclerosis-in-tunisian-patients
#3
Nadia Ben Fredj, Mouna Aissi, Walid Ben Selma, Imen Mahmoud, Faten Nefzi, Mahbouba Frih-Ayed, Jalel Boukadida, Mahjoub Aouni
Interleukin-10 (IL-10), a potent anti-inflammatory T-cell cytokine, has been shown to be a regulatory cytokine that is associated with disease remission in multiple sclerosis (MS) and exerts its activity through its cognate cell surface receptor complex, IL-10 receptor 1 (IL-10R1) and IL-10R2. The purpose of this study was to investigate the IL-10R1 S138G loss-of-function polymorphism (A536G: rs3135932) for possible influence on susceptibility and outcome of MS in Tunisian patients. A total of 103 Tunisian MS patients and 160 control subjects were studied...
February 22, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28224137/hmgb1-anti-hmgb1-antibodies-define-a-molecular-signature-of-early-stages-of-hiv-associated-neurocognitive-isorders-hand
#4
Marie-Lise Gougeon, Béatrice Poirier-Beaudouin, Jacques Durant, Christine Lebrun-Frenay, Héla Saïdi, Valérie Seffer, Michel Ticchioni, Stephane Chanalet, Helene Carsenti, Alexandra Harvey-Langton, Muriel Laffon, Jacqueline Cottalorda, Christian Pradier, Pierre Dellamonica, Matteo Vassallo
BACKGROUND: HIV-associated neurocognitive disorders (HAND) persist in the post-HAART era, characterized by asymptomatic neurocognitive impairment (ANI) and mild neurocognitive disorders (MND). High mobility group box 1 (HMGB1) is a non-histone chromosomal protein widely expressed in the nucleus of all eukaryotic cells, including brain cells, which acts as a potent proinflammatory cytokine when actively secreted from immune cells. Recent reports suggested that HMGB1 acts on microglial cells to promote neuroinflammation...
February 2017: Heliyon
https://www.readbyqxmd.com/read/28224132/dataset-of-longitudinal-analysis-of-tear-cytokine-levels-cd4-cd8-counts-and-hiv-viral-load-in-dry-eye-patients-with-hiv-infection
#5
Praveen Kumar Balne, Rupesh Agrawal, Veonice Bijin Au, Bernett Lee, Eileen Loo, Louis Tong, Arkasubhra Ghosh, Stephen C Teoh, John Connolly, Petrina Tan
The data presented in this article shows the longitudinal analysis of tear fluid cytokine profiles, blood CD4 and CD8 counts and HIV viral load in 34 dry eye patients with HIV infection during the HAART therapy. Clinical samples were collected from HIV patients with dry eye disease at the time of presentation to the clinic (visit 1), three months (visit 2) and 6 months (visit 3) after the presentation. At each time point tear samples were evaluated for 41 cytokines using Luminex bead based multiplex assay and blood samples were tested for HIV viral load and CD4 and CD8 counts...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28224047/identification-of-extracellular-matrix-components-and-biological-factors-in-micronized-dehydrated-human-amnion-chorion-membrane
#6
Jennifer Lei, Lauren B Priddy, Jeremy J Lim, Michelle Massee, Thomas J Koob
Objective: The use of bioactive extracellular matrix (ECM) grafts such as amniotic membranes is an attractive treatment option for enhancing wound repair. In this study, the concentrations, activity, and distribution of matrix components, growth factors, proteases, and inhibitors were evaluated in PURION(®) Processed, micronized, dehydrated human amnion/chorion membrane (dHACM; MiMedx Group, Inc.). Approach: ECM components in dHACM tissue were assessed by using immunohistochemical staining, and growth factors, cytokines, proteases, and inhibitors were quantified by using single and multiplex ELISAs...
February 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28220270/multiplex-assessment-of-serum-cytokine-and-chemokine-levels-in-idiopathic-morphea-and-vitamin-k1-induced-morphea
#7
Lori Ann Cox, Guy F Webster, Sonsoles Piera-Velazquez, Sergio A Jimenez
The levels of 63 cytokines, chemokines, and growth factors were measured in the serum of four patients with idiopathic morphea and of one patient with vitamin K1-induced morphea employing a multiplex assay to identify the role of inflammatory/immunologic events in their pathogenesis. Full-thickness skin biopsies of affected skin were analyzed by histopathology. Luminex assays for 63 cytokines, chemokines, and growth factors were performed in the sera from four patients with idiopathic morphea and in two different samples of serum obtained in two separate occasions from one patient with vitamin K1-induced morphea...
February 20, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28210632/increased-interleukin-17f-is-associated-with-elevated-autoantibody-levels-and-more-clinically-relevant-than-interleukin-17a-in-primary-sj%C3%A3-gren-s-syndrome
#8
Yuzhou Gan, Xiaozhen Zhao, Jing He, Xu Liu, Yun Li, Xiaolin Sun, Zhanguo Li
Th17 related immune response is pathogenic in primary Sjögren's syndrome (pSS). However, the role of IL-17F, one potent inflammatory member of IL-17 family cytokines in pSS, has not been specifically defined. We recruited one hundred and nine pSS patients and forty-two healthy controls and their serum levels of IL-17A and IL-17F were determined by multiplex cytokine assays. White blood cell, red blood cell, neutrophil, lymphocyte, IgM, IgG, C3, C4, RF, ANA, anti-SSA antibody, and anti-SSB antibody were measured by standard laboratory techniques...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28210014/dilemma-correlation-between-serum-level-of-hepcidin-and-il-6-in-anemic-myeloma-patients
#9
Lejla Ibricevic-Balic, Emina Icindic-Nakas, Sabaheta Hasic, Emina Kiseljakovic, Alma Sofo-Hafizovic, Sefkija Balic
INTRODUCTION: Anemia occurs in 60% to 80 % of patients with newly diagnosed myeloma multiplex (MM). The cause of anemia in MM is probably multi factorial and involved among the others hepcidin and some cytokines, especially interleukine-6. Anemia in MM is one of the risk factor used in Durie-Salmon classification for staging and prognostic score. Treatment options are set according to this score with most significant impact on survival. AIM: To estimate baseline level of serum hepcidin, IL-6 and iron metabolism markers in anemic MM patients, possible role of hepcidin and its interaction with IL-6...
December 2016: Medical Archives
https://www.readbyqxmd.com/read/28207941/mfg-e8-inhibits-periodontitis-in-non-human-primates-and-its-gingival-crevicular-fluid-levels-can-differentiate-periodontal-health-from-disease-in-humans
#10
Tetsuhiro Kajikawa, Fatimah Meshikhes, Tomoki Maekawa, Evlambia Hajishengallis, Kavita B Hosur, Toshiharu Abe, Kevin Moss, Triantafyllos Chavakis, George Hajishengallis
AIM: We have previously shown that the secreted glycoprotein MFG-E8 has anti-inflammatory and anti-osteoclastogenic properties. Our objective was to investigate the potential of MFG-E8 as a diagnostic or therapeutic agent in periodontitis. MATERIALS AND METHODS: Periodontitis was induced in non-human primates (NHPs) by placing ligatures around posterior teeth on both halves of the mandible for a split-mouth design: one side was treated with MFG-E8-Fc and the other with Fc control...
February 16, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28207826/changes-of-plasma-cytokines-and-chemokines-expression-level-in-nasopharyngeal-carcinoma-patients-after-treatment-with-definitive-intensity-modulated-radiotherapy-imrt
#11
Ya-Bin Jin, Guo-Yi Zhang, Kai-Rong Lin, Xiang-Ping Chen, Jin-Huan Cui, Yue-Jian Wang, Wei Luo
BACKGROUND: Potential clinical application values of certain cytokines and chemokines that participate in the process of tumor growth, invasion, and metastasis have been reported. However, there still lack of biomarkers for a great many of malignancy. This study identified cytokines or chemokines involved in the occurrence and development of nasopharyngeal carcinoma (NPC), which might be a biomarker for noninvasive early diagnosis. METHODS: The plasma levels of 19 cytokines and chemokines were detected by the luminex liquid array-based multiplexed immunoassays in 39 NPC patients before and after treatment by definitive intensity-modulated radiotherapy (IMRT)...
2017: PloS One
https://www.readbyqxmd.com/read/28205383/association-between-leukocyte-telomere-length-and-angiogenic-cytokines-in-knee-osteoarthritis
#12
Thitiya Poonpet, Natthaphon Saetan, Aree Tanavalee, Vajara Wilairatana, Pongsak Yuktanandana, Sittisak Honsawek
AIM: The aims of this study were to compare leukocyte relative telomere length (RTL) in knee osteoarthritis (OA) patients and healthy controls and to investigate associations between plasma angiogenic cytokine concentrations and leukocyte RTL. METHOD: Eighty knee OA patients and 60 age-matched controls were enrolled. Leukocyte RTL was assessed using real-time quantitative polymerase chain reaction (qPCR). Angiogenic cytokines were measured by a multiplex immunoassay...
February 16, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28202084/aprepitant-limits-in-vivo-neuroinflammatory-responses-in-a-rhesus-model-of-lyme-neuroborreliosis
#13
Alejandra N Martinez, Amanda R Burmeister, Geeta Ramesh, Lara Doyle-Meyers, Ian Marriott, Mario T Philipp
BACKGROUND: Substance P (SP) is produced at high levels in the central nervous system (CNS), and its target receptor, neurokinin 1 receptor (NK-1R), is expressed by glia and leukocytes. This tachykinin functions to exacerbate inflammatory responses at peripheral sites. Moreover, SP/NK-1R interactions have recently been associated with severe neuroinflammation and neuronal damage. We have previously demonstrated that NK-1R antagonists can limit neuroinflammatory damage in a mouse model of bacterial meningitis...
February 15, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28192876/determination-of-16-serum-angiogenic-factors-in-stage-i-non-small-cell-lung-cancer-using-a-bead-based-multiplex-immunoassay
#14
Agnieszka Klupczynska, Paweł Dereziński, Jan Matysiak, Wojciech Dyszkiewicz, Mariusz Kasprzyk, Zenon J Kokot
The aim of the study was to identify significant abnormalities in angiogenic factor profiles occurring at early-stage non-small cell lung cancer (NSCLC). Contrary to the previous studies, our research included patients with only stage I NSCLC, which allowed for estimating the utility of circulating angiogenic factors in early NSCLC detection. The investigation was performed in serum samples collected from individuals with untreated NSCLC (n=41) and a matched control group of healthy individuals (n=61). All patients had histopathologically-confirmed stage IA or IB NSCLC...
February 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28189042/activity-of-inflammatory-bowel-disease-influences-the-expression-of-cytokines-in-gingival-tissue
#15
C M Figueredo, A P Martins, R Lira-Junior, J B Menegat, A T Carvalho, R G Fischer, A Gustafsson
This study assessed the cytokine expression in gingival and intestinal tissues from periodontitis patients with inflammatory bowel disease (IBD) and evaluated if IBD activity is a covariate to the amount of gingival cytokines. Paired gingival and intestinal tissues were collected from 21 patients and homogenised using a cell disruptor. Cytokine expression (IL-1β, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, IFN-γ, sCD40L, and TNF-α) was evaluated using bead-based multiplex technology...
February 8, 2017: Cytokine
https://www.readbyqxmd.com/read/28186488/control-of-immune-ligands-by-members-of-a-cytomegalovirus-gene-expansion-suppresses-natural-killer-cell-activation
#16
Ceri Alan Fielding, Michael P Weekes, Luis V Nobre, Eva Ruckova, Gavin S Wilkie, Joao A Paulo, Chiwen Chang, Nicolás M Suárez, James A Davies, Robin Antrobus, Richard J Stanton, Rebecca J Aicheler, Hester Nichols, Borek Vojtesek, John Trowsdale, Andrew J Davison, Steven P Gygi, Peter Tomasec, Paul J Lehner, Gavin Wg Wilkinson
The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel NK cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1,300 cell surface and ~7,200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors...
February 10, 2017: ELife
https://www.readbyqxmd.com/read/28185353/human-vaginal-epithelial-cells-augment-autophagy-marker-genes-in-response-to-candida-albicans-infection
#17
Ankit Shroff, Roicy Sequeira, Kudumula Venkata Rami Reddy
PROBLEM: Autophagy plays an important role in clearance of intracellular pathogens. However, no information is available on its involvement in vaginal infections such as vulvo-vaginal candidiasis (VVC). VVC is intimately associated with the immune status of the human vaginal epithelial cells (VECs). The objective of our study is to decipher if autophagy process is involved during Candida albicans infection of VECs. METHODS OF STUDY: In this study, C. albicans infection system was established using human VEC line (VK2/E6E7)...
February 10, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28182293/standardizing-the-freeze-thaw-preparation-of-growth-factors-from-platelet-lysate
#18
Gabriel Strandberg, Felix Sellberg, Pehr Sommar, Martin Ronaghi, Norbert Lubenow, Folke Knutson, David Berglund
BACKGROUND: Over the past decades, the focus on the regenerative properties of platelets (PLTs) has intensified and many PLT-derived growth factors are readily used in medical settings. A general lack of standardization in the preparation of these growth factors remains, however, and this study therefore examines the dynamics of growth factors throughout the freeze-thaw procedure. STUDY DESIGN AND METHODS: Plateletpheresis (PA) and PLT-poor plasma (PPP) samples were collected from 10 healthy donors...
February 9, 2017: Transfusion
https://www.readbyqxmd.com/read/28178994/doxycycline-attenuates-breast-cancer-related-inflammation-by-decreasing-plasma-lysophosphatidate-concentrations-and-inhibiting-nf-%C3%AE%C2%BAb-activation
#19
Xiaoyun Tang, Xianyan Wang, Yuan Y Zhao, Jonathan M Curtis, David N Brindley
BACKGROUND: We previously discovered that tetracyclines increase the expression of lipid phosphate phosphatases at the surface of cells. These enzymes degrade circulating lysophosphatidate and therefore doxycycline increases the turnover of plasma lysophosphatidate and decreases its concentration. Extracellular lysophosphatidate signals through six G protein-coupled receptors and it is a potent promoter of tumor growth, metastasis and chemo-resistance. These effects depend partly on the stimulation of inflammation that lysophosphatidate produces...
February 8, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28178290/prenatal-exposure-to-respiratory-syncytial-virus-alters-postnatal-immunity-and-airway-smooth-muscle-contractility-during-early-life-reinfections
#20
Paul M Brown, Terri J Harford, Vandana Agrawal, Belinda Yen-Lieberman, Fariba Rezaee, Giovanni Piedimonte
Maternal viral infections can have pathological effects on the developing fetus which last long after birth. Recently, maternal-fetal transmission of respiratory syncytial virus (RSV) was shown to cause postnatal airway hyperreactivity (AHR) during primary early-life reinfection; however, the influence of prenatal exposure to RSV on offspring airway immunity and smooth muscle contractility during recurrent postnatal reinfections remains unknown. Therefore, we sought to determine whether maternal RSV infection impairs specific aspects of cell-mediated offspring immunity during early-life reinfections and the mechanisms leading to AHR...
2017: PloS One
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