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Multiplex and cytokine

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https://www.readbyqxmd.com/read/29034249/misoprostol-inhibits-lipopolysaccharide-induced-pro-inflammatory-cytokine-production-by-equine-leukocytes
#1
Emily Medlin Martin, Kristen M Messenger, Mary Katherine Sheats, Samuel L Jones
Pro-inflammatory cytokines including tumor necrosis factor α (TNFα), IL-1β, IL-6, and IL-8 are potent immune mediators that exacerbate multiple equine diseases such as sepsis and laminitis. Unfortunately, safe and effective cytokine-targeting therapies are lacking in horses; therefore, novel mechanisms of inhibiting cytokine production are critically needed. One potential mechanism for inhibiting cytokine synthesis is elevation of intracellular cyclic AMP (cAMP). In human leukocytes, intracellular cAMP production is induced by activation of E-prostanoid (EP) receptors 2 and 4...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29032986/mycobacterium-tuberculosis-strains-induce-strain-specific-cytokine-and-chemokine-response-in-pulmonary-epithelial-cells
#2
Nontobeko E Mvubu, Balakrishna Pillay, Lyle R McKinnon, Manormoney Pillay
M. tuberculosis F15/LAM4/KZN has been associated with high transmission rates of drug resistant tuberculosis in the KwaZulu-Natal province of South Africa. The current study elucidated the cytokine/chemokine responses induced by representatives of the F15/LAM4/KZN and other dominant strain families in pulmonary epithelial cells. Multiplex cytokine analyses were performed at 24, 48 and 72h post infection of the A549 pulmonary epithelial cell line with the F15/LAM4/KZN, F28, F11, Beijing, Unique and H37Rv strains at an MOI of ∼10:1...
October 9, 2017: Cytokine
https://www.readbyqxmd.com/read/29030692/changes-in-aqueous-concentrations-of-various-cytokines-after-intravitreal-bevacizumab-and-subtenon-triamcinolone-injection-for-diabetic-macular-edema
#3
Seung-Young Yu, Dong Heun Nam, Dae Yeong Lee
PURPOSE: The purpose of this study was to compare the changes in the aqueous cytokine levels after intravitreal bevacizumab with those after combined intravitreal bevacizumab and subtenon triamcinolone injection in diabetic macular edema (DME). METHODS: This study examined 24 eyes of 23 patients with DME. Each patient with DME received randomly either an intravitreal injection of bevacizumab (IVBe) or IVBe with a subtenon triamcinolone injection (IVBe + STTA)...
October 13, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29029618/balneotherapeutic-effects-of-high-mineral-spring-water-on-the-atopic-dermatitis-like-inflammation-in-hairless-mice-via-immunomodulation-and-redox-balance
#4
Johny Bajgai, Ailyn Fadriquela, Jesmin Ara, Rahima Begum, Md Faruk Ahmed, Cheol-Su Kim, Soo-Ki Kim, Kwang-Yong Shim, Kyu-Jae Lee
BACKGROUND: Atopic dermatitis (AD) is a chronic relapsing allergic inflammatory skin disease that currently affects millions of children and adults worldwide. Drugs used to treat these inflammatory diseases include anti-histamines, corticosteroids and calcineurin inhibitors but these drugs have their limitations such as adverse effects with their long-term usage. Thus, researcher's interest in several alternative and complementary therapies are continually growing and balneotherapy is one of these approaches...
October 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29028771/mechanisms-of-thrombocytopenia-during-septic-shock-a-multiplex-cluster-analysis-of-endogenous-sepsis-mediators
#5
Alexandre Bedet, Keyvan Razazi, Florence Boissier, Mathieu Surenaud, Sophie Hue, Stéphane Giraudier, Christian Brun-Buisson, Armand Mekontso Dessap
BACKGROUND: Thrombocytopenia is a common feature of sepsis and may involve various mechanisms often related to the inflammatory response. This study aimed at evaluating factors associated with thrombocytopenia during human septic shock. In particular, we used a multiplex analysis to assess the role of endogenous sepsis mediators. METHODS: Prospective, observational study. Thrombocytopenia was defined as an absolute platelet count <100 G/L or a 50% relative decrease in platelet count during the first week of septic shock...
October 12, 2017: Shock
https://www.readbyqxmd.com/read/29017774/an-alternative-biomarker-based-approach-for-the-prediction-of-proteins-known-to-sensitize-the-respiratory-tract
#6
Kathrin S Zeller, Henrik Johansson, Trine Østergaard Lund, Nanna N Kristensen, Erwin L Roggen, Malin Lindstedt
Many natural and industrial proteins are known to have properties that can result in type I hypersensitivity, however, to date, no validated test system exists that can predict the sensitizing potential of these allergens. Thus, the objective of this study was to develop a protocol based on the myeloid cell-based Genomic Allergen Rapid Detection (GARD) assay that can be used to assess and predict the capacity of protein allergens known to induce sensitization in the respiratory tract. Cellular responses induced by eight selected proteins were assessed using transcriptional profiling, flow cytometry and multiplex cytokine analysis...
October 7, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29017522/analysis-of-plasma-multiplex-cytokines-and-increased-level-of-il-10-and-il-1ra-cytokines-in-febrile-seizures
#7
Kyungmin Kim, Byungok Kwak, Aram Kwon, Jongseok Ha, Soojin Kim, Sunwhan Bae, Jaesung Son, Soonyung Kim, Ran Lee
BACKGROUND: Febrile seizures are the most common form of childhood seizures. Fever generation involves many cytokines, including both pro- and anti-inflammatory cytokines. Some of these cytokines also induce febrile seizures. We compared cytokine production in children with a fever alone (healthy control group) and febrile seizure children group. Also, we evaluated the cytokine level of children with a fever alone and febrile seizure history. METHODS: Fifty febrile seizure patients and 39 normal control patients who visited the emergency department of Konkuk University Hospital from December 2015 to December 2016 were included in this study...
October 10, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28991679/brainstem-cytokine-changes-in-healthy-ageing-and-motor-neurone-disease
#8
Anuradha Tennakoon, Viythia Katharesan, Ian P Johnson
Neuroinflammation is linked to healthy ageing, but its role in the development of age-related neurodegenerative diseases is unclear. In this pilot study we used a multiplex assay approach to compare 27 cytokines in 6 young adult and 6 ageing control brainstems with those in 6 MND brainstems. We report that healthy ageing is associated with significantly increased brainstem levels of IL-1β, IP-10 and MIP-1β which co-localise immunocytochemically to astrocytes. MND brainstem is also characterised by a general increase in both pro- and anti-cytokine levels, but fails to show the expected age-related increase in MIP-1β and IP-10...
October 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28988898/elevated-aqueous-cytokine-levels-in-eyes-with-ocular-surface-diseases
#9
Naohiko Aketa, Takefumi Yamaguchi, Teru Asato, Yukari Yagi-Yaguchi, Terumasa Suzuki, Kazunari Higa, Toshihide Kurihara, Yoshiyuki Satake, Kazuo Tsubota, Jun Shimazaki
PURPOSE: To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases. DESIGN: Prospective consecutive case series. METHODS: This study includes 14 patients (62.4±13.7 years old) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar) and 30 patients who underwent cataract surgery (healthy controls)...
October 5, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28978808/monoclonal-igg-in-mgus-and-multiple-myeloma-targets-infectious-pathogens
#10
Adrien Bosseboeuf, Delphine Feron, Anne Tallet, Cédric Rossi, Cathy Charlier, Laurent Garderet, Denis Caillot, Philippe Moreau, Marina Cardó-Vila, Renata Pasqualini, Wadih Arap, Alfreda Destea Nelson, Bridget S Wilson, Hélène Perreault, Eric Piver, Pierre Weigel, François Girodon, Jean Harb, Edith Bigot-Corbel, Sylvie Hermouet
Subsets of mature B cell neoplasms are linked to infection with intracellular pathogens such as Epstein-Barr virus (EBV), hepatitis C virus (HCV), or Helicobacter pylori. However, the association between infection and the immunoglobulin-secreting (Ig-secreting) B proliferative disorders remains largely unresolved. We investigated whether the monoclonal IgG (mc IgG) produced by patients diagnosed with monoclonal gammopathy of undetermined significance (MGUS) or multiple myeloma (MM) targets infectious pathogens...
October 5, 2017: JCI Insight
https://www.readbyqxmd.com/read/28973330/prehematopoietic-stem-cell-transplantation-tear-cytokines-as-potential-susceptibility-biomarkers-for-ocular-chronic-graft-versus-host-disease
#11
Lidia Cocho, Itziar Fernández, Margarita Calonge, Maite Sainz de la Maza, Montserrat Rovira, Michael E Stern, Carmen Garcia-Vazquez, Amalia Enríquez-de-Salamanca
Purpose: To determine if cytokine tear levels before hematopoietic stem cell transplantation (HSCT) can help anticipate the occurrence of ocular chronic graft-versus-host disease (cGVHD). Methods: In this pilot study, 25 patients undergoing HSCT were followed prospectively for ≤43 months. After ocular examinations, tears were collected before HSCT. Levels of 19 cytokines (epidermal growth factor [EGF], eotaxin 1/CCL11, fractalkine/CX3CL1, IL-1Ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12p70, IL-13, IL-17A, IP-10/CXCL10, IFN-γ, VEGF, TNF-α, and RANTES/CCL5) were measured by multiplex bead assay...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28969616/assessment-of-biomarkers-using-multiplex-assays-in-aqueous-humor-of-patients-with-diabetic-retinopathy
#12
Hui Chen, Xiongze Zhang, Nanying Liao, Feng Wen
BACKGROUND: With the high prevalence of type 2 diabetes, diabetic retinopathy (DR) has become a leading health problem worldwide. The pathogenesis of DR is complex and several vascular, inflammatory, and neuronal mechanisms are involved. The purpose of this study was to assess the levels of immune and inflammatory biomarkers in the aqueous humor of patients with different severities of DR and to analyze the correlations between Interleukin-6 (IL-6) and these biomarkers, and between IL-6 and the severity of the disease...
October 2, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28968855/cytokines-produced-in-response-to-varicella-zoster-virus-vzv-infection-of-arpe-19-cells-stimulate-lymphocyte-chemotaxis
#13
Chiharu Graybill, David J Claypool, John T Brinton, Myron J Levin, Katherine S Lee
Posterior uveitis is an ocular complication that can occur with reactivation of varicella-zoster virus (VZV). It may lead to loss of vision due to retinal detachment and chronic inflammation, which often causes more severe disease than the virus infection itself. To increase our understanding of the immune response, we infected the retinal pigment epithelial (RPE) cell line, ARPE-19, with cell-associated VZV and compared its response to that of the MeWo cell line using multiplex assays. We observed a difference in the magnitude and kinetics of cytokine responses between the two cell types, and differential migration of CD4+ and CD8+ T cells towards these cytokines...
August 18, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28962697/pentoxifylline-inhibits-lipopolysaccharide-induced-inflammatory-mediators-in-human-second-trimester-placenta-explants
#14
Esther M Speer, Xinhua Lin, Amitasrigowri Murthy, Wei Hou, Shahidul Islam, Nazeeh Hanna
BACKGROUND: Intrauterine infection and inflammation during pregnancy, which leads to up-regulation of inflammatory cytokines and prostaglandin synthesis, has been implicated in the pathogenesis of preterm delivery and other pregnancy complications. Effective preventive and therapeutic strategies to reduce these outcomes are lacking to date. Pentoxifylline (PTX) is a non-specific phosphodiesterase inhibitor which raises intracellular cyclic adenosine monophosphate and decreases production of pro-inflammatory mediators while enhancing anti-inflammatory cytokines...
October 2017: Placenta
https://www.readbyqxmd.com/read/28957547/increased-autophagy-contributes-to-the-inflammatory-phenotype-of-juvenile-idiopathic-arthritis-synovial-fluid-t-cells
#15
Janneke G C Peeters, Nienke de Graeff, Martin Lotz, Salvatore Albani, Sytze de Roock, Jorg van Loosdregt
Objectives: JIA is an autoimmune disease involving disturbed T-cell homeostasis, marked by highly activated effector T cells. Autophagy, a lysosomal degradation pathway, is crucial for maintaining cellular homeostasis by regulating the survival, differentiation and function of a large variety of cells, including T cells. The aim of this study was to examine the rate of autophagy in JIA T cells and to investigate the effect of inhibition of autophagy on the inflammatory phenotype of JIA T cells...
October 1, 2017: Rheumatology
https://www.readbyqxmd.com/read/28954820/cannabidiol-and-palmitoylethanolamide-are-anti-inflammatory-in-the-acutely-inflamed-human-colon
#16
Daniel G Couch, Chris Tasker, Elena Theophilidou, Jonathan N Lund, Saoirse E O'Sullivan
Objective: We sought to quantify the anti-inflammatory effects of two cannabinoid drugs: cannabidiol (CBD) and palmitoylethanolamide (PEA), in cultured cell lines and compared this effect with experimentally inflamed explant human colonic tissue.  These effects were explored in acutely and chronically inflamed colon, using inflammatory bowel disease and appendicitis explants. Design: Caco-2 cells and human colonic explants collected from elective bowel cancer, inflammatory bowel disease (IBD) or acute appendicitis resections, and were treated with the following drug treatments: vehicle, an inflammatory protocol of IFNγ and TNFα (10 ng/ml), inflammation and PEA (10µM), inflammation and CBD (10µM), & PEA or CBD alone...
September 27, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28953078/inflammatory-urinary-cytokine-expression-and-quality-of-life-in-patients-with-overactive-bladder
#17
Nirmala Pillalamarri, Dara F Shalom, Marjorie L Pilkinton, Harvey A Winkler, Prodyot K Chatterjee, Malvika Solanki, Christine N Metz
OBJECTIVE: The aims of this study were to analyze levels of selected inflammatory urinary cytokines/chemokines in subjects with overactive bladder (OAB) and to determine if cytokine/chemokine levels correlate with quality of life and symptom distress. METHODS: This prospective, case-control pilot analysis included 23 women with OAB and 22 control subjects. Overactive bladder subjects were enrolled if they had symptoms of urinary frequency, urgency, or urge incontinence for more than 3 months and urodynamic evidence of detrusor overactivity...
September 25, 2017: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/28942825/a-single-administration-of-fish-oil-inhibits-the-acute-inflammatory-response-in-rats
#18
Laura Lícia Milani de Arruda, Franciele Queiroz Ames, Damila Rodrigues de Morais, Renata Grespan, Ana Paula Maziero Gil, Maria Angélica Raffaini Covas Pereira Silva, Jesuí Vergílio Visentainer, Roberto Kenji Nakamura Cuman, Ciomar Aparecida Bersani-Amado
OBJECTIVE: To investigate the antiinflammatory effects of a single administration of fish oil (FO) on the acute inflammatory response. METHODS: The paw edema and pleurisy models were used to evaluate the effects of FO dissolved in olive oil (FOP) orally administered in a single dose in rats. Nitric oxide (NO) concentrations in the pleural exudate were performed according to the Griess method and the cytokine concentrations were determined by Luminex bead-based multiplex assay...
August 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28939412/th1-signatures-are-present-in-the-lower-airways-of-children-with-severe-asthma-regardless-of-allergic-status
#19
Julia A Wisniewski, Lyndsey M Muehling, Jacob D Eccles, Brian J Capaldo, Rachana Agrawal, Debbie-Ann Shirley, James T Patrie, Lisa J Workman, Alexander J Schuyler, Monica G Lawrence, W Gerald Teague, Judith A Woodfolk
BACKGROUND: The pathogenesis of severe asthma in childhood remains poorly understood. OBJECTIVE: To construct the immunological landscape in the airways of children with severe asthma. METHODS: Comprehensive analysis of multiple cell types and mediators was performed by flow cytometry and multiplex assay using bronchoalveolar lavage (BAL) specimens (n=68) from 52 highly characterized allergic and non-allergic children (0.5-17 years) with severe treatment-refractory asthma...
September 19, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28939262/exercise-training-improves-the-il-10-tnf-%C3%AE-cytokine-balance-in-the-gastrocnemius-of-rats-with-heart-failure
#20
Leonardo Calegari, Ramiro B Nunes, Bruna B Mozzaquattro, Douglas D Rossato, Pedro Dal Lago
OBJECTIVE: This study examined the effects of exercise training (ExT) upon concentration of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) in the gastrocnemius of rats with heart failure (HF) induced by left coronary artery ligation. METHODS: Adult male Wistar rats submitted to myocardial infarction (MI) or sham surgery were randomly allocated into one of four experimental groups: trained HF (Tr-HF), sedentary HF (Sed-HF), trained sham (Tr-Sham) and sedentary sham (Sed-Sham)...
September 7, 2017: Brazilian Journal of Physical Therapy
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