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https://www.readbyqxmd.com/read/28642451/il-10-plays-a-pivotal-role-in-tamoxifen-induced-spasmolytic-polypeptide-expressing-metaplasia-in-gastric-mucosa
#1
Chansu Lee, Hyuk Lee, Seo Yun Hwang, Chang Mo Moon, Sung Noh Hong
Background/Aims: Gastric cancer evolves in the pathologic mucosal milieu, and its development is characterized by both the loss of acid-secreting parietal cells and mucosal cell metaplasia, called spasmolytic polypeptide-expressing metaplasia (SPEM). Cytokines, such as interleukin (IL)-10, IL-1β, and IL-6, play a key role in gastric carcinogenesis. However, changes in the cytokine profile of SPEM have not been evaluated. Methods: To induce SPEM in mouse stomachs, C57BL/6 mice were intraperitoneally injected with tamoxifen and sacrificed at 3, 10, and 21 days after treatment...
June 26, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28637178/the-at04a-vaccine-against-proprotein-convertase-subtilisin-kexin-type-9-reduces-total-cholesterol-vascular-inflammation-and-atherosclerosis-in-apoe-3leiden-cetp-mice
#2
Christine Landlinger, Marianne G Pouwer, Claudia Juno, José W A van der Hoorn, Elsbet J Pieterman, J Wouter Jukema, Guenther Staffler, Hans M G Princen, Gergana Galabova
Aims: Proprotein convertase subtilisin/kexin type 9 (PCSK9) has emerged as a promising therapeutic target for the treatment of hypercholesterolaemia and atherosclerosis. PCSK9 binds to the low density lipoprotein receptor and enhances its degradation, which leads to the reduced clearance of low density lipoprotein cholesterol (LDLc) and a higher risk of atherosclerosis. In this study, the AT04A anti-PCSK9 vaccine was evaluated for its therapeutic potential in ameliorating or even preventing coronary heart disease in the atherogenic APOE*3Leiden...
June 19, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28633757/the-relationship-between-circulating-mitochondrial-dna-and-inflammatory-cytokines-in-patients-with-major-depression
#3
Yuki Kageyama, Takaoki Kasahara, Masaki Kato, Shiho Sakai, Yasuhiko Deguchi, Munehide Tani, Kenji Kuroda, Kotaro Hattori, Sumiko Yoshida, Yuichi Goto, Toshihiko Kinoshita, Koki Inoue, Tadafumi Kato
BACKGROUND: Although inflammatory cytokines are established biomarkers of mood disorders, their molecular mechanism is not known. We hypothesized that circulating mitochondrial DNA (mtDNA) contributes to inflammation and could be used as biomarkers. We investigated if circulating mtDNA level is associated with inflammatory cytokines and can be used as a biomarker of mood disorders. METHODS: Plasma mtDNA concentration was measured with real-time quantitative PCR targeting two regions of the mtDNA and plasma levels of four cytokines (GM-CSF, IL-2, IL-4, and IL-6) were measured with a multiplex immunoassay method in 109 patients with major depressive disorder (MDD)...
June 6, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28630342/autoantibody-profiling-on-a-plasmonic-nano-gold-chip-for-the-early-detection-of-hypertensive-heart-disease
#4
Xiaoyang Li, Tatiana Kuznetsova, Nicholas Cauwenberghs, Matthew Wheeler, Holden Maecker, Joseph C Wu, Francois Haddad, Hongjie Dai
The role of autoimmunity in cardiovascular (CV) diseases has been increasingly recognized. Autoimmunity is most commonly examined by the levels of circulating autoantibodies in clinical practices. Measurement of autoantibodies remains, however, challenging because of the deficiency of reproducible, sensitive, and standardized assays. The lack of multiplexed assays also limits the potential to identify a CV-specific autoantibody profile. To overcome these challenges, we developed a nanotechnology-based plasmonic gold chip for autoantibody profiling...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628884/proteomics-profiling-reveals-inflammatory-biomarkers-of-antidepressant-treatment-response-findings-from-the-co-med-trial
#5
Bharathi S Gadad, Manish K Jha, Bruce D Grannemann, Taryn L Mayes, Madhukar H Trivedi
Animal and human studies suggest an association between depression and aberrant immune response. Further, common inflammatory markers may change during the course of antidepressant treatment in patients. The objective of this study was to evaluate changes in inflammatory markers and clinical outcomes from subjects enrolled in the Combining Medications to Enhance Depression Outcome (CO-MED) trial. At baseline and week 12 (treatment completion), plasma samples of 102 participants were analyzed via a multiplex assay comprised of inflammatory markers using a 27-plex standard assay panel plus a 4-plex human acute phase xMAP technology based platform...
May 26, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28628633/pro-inflammatory-immune-responses-are-associated-with-clinical-signs-and-symptoms-of-human-anaplasmosis
#6
Anna M Schotthoefer, Steven J Schrodi, Jennifer K Meece, Thomas R Fritsche, Sanjay K Shukla
Human anaplasmosis (HA) is an emerging tick-borne disease that may present as a mild flu-like illness or a life threatening, sepsis-like condition. Although disease severity is hypothesized to relate to immunopathology and immune dysfunction in humans, studies to directly measure immune responses in infected humans have been very limited. We quantified cytokines in 80 confirmed HA patients using a multiplex chemiluminescence immunoassay system and compared similarly measured responses in 1000 control subjects...
2017: PloS One
https://www.readbyqxmd.com/read/28623311/identification-of-rare-genetic-variation-of-nlrp1-gene-in-familial-multiple-sclerosis
#7
Ales Maver, Polona Lavtar, Smiljana Ristić, Sanja Stopinšek, Saša Simčič, Keli Hočevar, Juraj Sepčić, Jelena Drulović, Tatjana Pekmezović, Ivana Novaković, Hodžić Alenka, Gorazd Rudolf, Saša Šega, Nada Starčević-Čizmarević, Anja Palandačić, Gordana Zamolo, Miljenko Kapović, Tina Likar, Borut Peterlin
The genetic etiology and the contribution of rare genetic variation in multiple sclerosis (MS) has not yet been elucidated. Although familial forms of MS have been described, no convincing rare and penetrant variants have been reported to date. We aimed to characterize the contribution of rare genetic variation in familial and sporadic MS and have identified a family with two sibs affected by concomitant MS and malignant melanoma (MM). We performed whole exome sequencing in this primary family and 38 multiplex MS families and 44 sporadic MS cases and performed transcriptional and immunologic assessment of the identified variants...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622063/characteristic-profiles-of-inflammatory-cytokines-in-the-aqueous-humor-of-glaucomatous-eyes
#8
Taiki Kokubun, Satoru Tsuda, Hiroshi Kunikata, Masayuki Yasuda, Noriko Himori, Shiho Kunimatsu-Sanuki, Kazuichi Maruyama, Toru Nakazawa
PURPOSE: To analyze cytokine profiles of the aqueous humor of eyes with primary open-angle glaucoma (POAG), neovascular glaucoma (NVG), and cataract (as controls). METHODS: A multiplex bead assay was used to measure concentrations of 27 cytokines in aqueous humor samples from 54 eyes. RESULTS: Detection rates were as follows: IL-7: NVG higher than POAG; IL-10: POAG lower than cataract or NVG; and GM-CSF: cataract higher than POAG or NVG. Concentrations were as follows: IL-8, IP-10, MCP-1, and MIP-1β: POAG and NVG higher than cataract; IL-9: POAG lower than NVG; IL-12: POAG lower than cataract or NVG; and VEGF: NVG higher than cataract or POAG and POAG lower than cataract...
June 16, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28620678/effect-of-lifestyle-improvement-program-on-the-biomarkers-of-adiposity-inflammation-and-gut-hormones-in-overweight-obese-asian-indians-with-prediabetes
#9
Kuppan Gokulakrishnan, Harish Ranjani, Mary Beth Weber, Gautam Kumar Pandey, Ranjit Mohan Anjana, Muthuswamy Balasubramanyam, Dorairaj Prabhakaran, Nikhil Tandon, K M Venkat Narayan, Viswanathan Mohan
AIMS: While lifestyle modification is known to offer several metabolic benefits, there is paucity of comprehensive data on changes in biomarkers of adiposity, inflammation as well as gut hormones. We investigated these biomarkers in overweight/obese individuals with prediabetes randomized to either 4 months of a lifestyle improvement program or standard care and followed them up for a year. METHODS: Participants [standard care and intervention arm (n = 75 each)] were randomly selected from the Diabetes Community Lifestyle Improvement Program trial...
June 15, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/28617371/-cognitive-dysfunction-and-content-of-inflammatory-markers-in-patients-after-coronary-artery-bypass-graft
#10
D A Ovchinnikov, D D Amosov, E A Vorobyov, V V Garnyuk, P P Beltiukov, V K Grebennik, M L Gordeev, E R Barantsevich
AIM: To study the relationship between the content of inflammatory biomarkers and cognitive function in patients after coronary artery bypass graft (CABG) performed in condition of artificial blood circulation (ABC) or open-heart surgery (OHS). MATERIAL AND METHODS: Twenty-nine patients with ischemic heart disease who survived CABG, mean age 62.4±6.2 years, were studied. The ABC group (n=18) and OHS group (n=11) were matched for age and sex. Patients underwent standard clinical examination as well as neurological examination and neuropsychological testing...
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/28610607/resveratrol-distinctively-modulates-the-inflammatory-profiles-of-immune-and-endothelial-cells
#11
Joseph Schwager, Nathalie Richard, Franziska Widmer, Daniel Raederstorff
BACKGROUND: The phenolic substance resveratrol (RES) is a plant metabolite known to modulate numerous physiological functions and to exert beneficial effects as a cancer-chemopreventing agent and on neurological, hepatic, and cardiovascular systems. Since the compound affects the lifespan of yeast and flies it might be an anti-aging substance. Mechanistically, RES is involved in down regulating the inflammatory response. The pleiotropic effects of RES in cells of the immune and endothelial system were examined in this study...
June 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28609384/cytokine-profiles-in-osteoporosis-suggest-a-proresorptive-bias
#12
Fawaz Azizieh, Raj Raghupathy, Diaa Shehab, Khaled Al-Jarallah, Renu Gupta
OBJECTIVE: As the immune system is suggested to contribute to the pathophysiology of osteoporosis in menopause, we compared the levels of proresorptive and antiresorptive cytokines produced by peripheral blood mononuclear cells (PBMCs) from postmenopausal women with normal and low bone mineral density (BMD). METHODS: Seventy-one postmenopausal women were studied; 25 had normal BMD and 46 had low BMD. Participants were categorized as normal (n = 25), osteopenic (n = 31), and osteoporotic (n = 15) based on T-scores...
June 19, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28602832/fumarate-decreases-edema-volume-and-improves-functional-outcome-after-experimental-stroke
#13
Bettina Hjelm Clausen, Louise Lundberg, Minna Yli-Karjanmaa, Nellie Anne Martin, Martina Svensson, Maria Zeiler Alfsen, Simon Bertram Flæng, Kristina Lyngsø, Antonio Boza-Serrano, Helle H Nielsen, Pernille B Hansen, Bente Finsen, Tomas Deierborg, Zsolt Illes, Kate Lykke Lambertsen
BACKGROUND: Oxidative stress and inflammation exacerbate tissue damage in the brain after ischemic stroke. Dimethyl-fumarate (DMF) and its metabolite monomethyl-fumarate (MMF) are known to stimulate anti-oxidant pathways and modulate inflammatory responses. Considering these dual effects of fumarates, we examined the effect of MMF treatment after ischemic stroke in mice. METHODS: Permanent middle cerebral artery occlusion (pMCAO) was performed using adult, male C57BL/6 mice...
June 8, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28600966/inflammatory-cytokines-in-normal-and-irreversibly-inflamed-pulps-a-systematic-review
#14
REVIEW
Vivian Hirsch, Michael Wolgin, Aleksandr V Mitronin, Andrej M Kielbassa
OBJECTIVE: To review the available literature in regard to the inflammatory process and pulpitis. Setting forth to evaluate if differences in the levels of various cytokines (TNF-α, IL-1β, IL-2, IL-6 and IL-8) can be observed in clinically diagnosed normal and irreversibly inflamed pulps that could serve as possible markers and/or diagnostic tools to predict and differentiate between certain states of inflammation. Methods used to measure and assess levels of cytokines have been limited to two protein quantification methods ELISA and/or Multiplex Array...
June 1, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28600710/the-influence-of-systemic-renin-angiotensin-inhibition-on-ocular-cytokines-related-to-proliferative-vitreoretinopathy
#15
Robert Hoerster, Sascha Fauser, Claus Cursiefen, Bernd Kirchhof, Ludwig M Heindl
PURPOSE: The renin-angiotensin system is involved in the pathogenesis of fibrosis in several organs via induction of transforming growth factor (TGF) beta. In the pathogenesis of proliferative vitreoretinopathy (PVR) TGF-beta plays a pivotal role, promoting transition of retinal pigment epithelial (RPE) cells into myofibroblasts. We studied the influence of angiotensin converting enzyme-inhibition (ACEI) on cytokines and growth factors, related to PVR in aqueous humor. METHODS: We performed a post hoc analysis of a prospectively conducted interventional case series...
June 9, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28595117/effect-of-the-anticoagulant-storage-time-and-temperature-of-blood-samples-on-the-concentrations-of-27-multiplex-assayed-cytokines-consequences-for-defining-reference-values-in-healthy-humans
#16
Linda Torrissen Hennø, Elin Storjord, Dorte Christiansen, Grete Bergseth, Judith Krey Ludviksen, Hilde Fure, Svein Barene, Erik Waage-Nielsen, Tom E Mollnes, Ole-Lars Brekke
Cytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently critically important to measure the in vivo levels. We therefore examined the effects of four different anticoagulants, EDTA, citrate, lepirudin, heparin compared to serum, on the levels of 27 different cytokines. The effects of storage temperature, freezing and thawing on the plasma cytokines were examined...
June 5, 2017: Cytokine
https://www.readbyqxmd.com/read/28593439/clinical-and-histopathological-amelioration-of-experimental-autoimmune-encephalomyelitis-by-aav-vectors-expressing-a-soluble-interleukin-23-receptor
#17
Marta Miralles, Herena Eixarch, Marcos Tejero, Carme Costa, Keiji Hirota, A Raul Castaño, Meritxell Puig, Gitta Stockinger, Xavier Montalban, Assumpció Bosch, Carmen Espejo, Miguel Chillon
The role of the T helper (Th)17 pathway has been clearly demonstrated in the onset and progression of autoimmune diseases, where interleukin (IL)-23 is a key molecule in maintaining the response mediated by Th17 cells. As a consequence, recent strategies based on blocking the interaction between IL-23 and its receptor (IL-23R), for example the anti-p19 antibody tildrakizumab, have been developed to regulate the Th17 pathway from the initial stages of the disease. Here, a soluble (s)IL-23R cDNA was cloned in expression plasmids and viral vectors...
June 7, 2017: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/28578701/distinct-pathophysiological-cytokine-profiles-for-discrimination-between-autoimmune-pancreatitis-chronic-pancreatitis-and-pancreatic-ductal-adenocarcinoma
#18
Sahar Ghassem-Zadeh, Matthias M Gaida, Szilard Szanyi, Hans Acha-Orbea, Jean-Louis Frossard, Ulf Hinz, Thilo Hackert, Oliver Strobel, Klaus Felix
BACKGROUND: Discriminating between autoimmune pancreatitis (AIP), chronic pancreatitis (CP), and pancreatic ductal adenocarcinoma (PDAC) can be challenging. In this retrospective study, levels of serum and tissue cytokines were analyzed as part of the clinical strategy for the preoperative differentiation between AIP and PDAC. The identification of differential cytokine profiles may help to prevent unnecessary surgical resection and allow optimal treatment of these pathologies. METHODS: To compare the cytokine profiles of AIP, CP, and PDAC patients, serum and pancreatic tissue homogenates were subjected to multiplex analysis of 17 inflammatory mediators...
June 2, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28573506/estradiol-has-differential-effects-on-acute-colonic-inflammation-in-the-presence-and-absence-of-estrogen-receptor-%C3%AE-expression
#19
Cameron M Armstrong, Kimberly F Allred, Brad R Weeks, Robert S Chapkin, Clinton D Allred
BACKGROUND: Inflammatory bowel disease (IBD) increases the risk of developing colon cancer. This risk is higher in men compared to women, implicating a role for female hormones in the protection against this disease. Studies from our laboratory demonstrated that estradiol (E2) protects against inflammation-associated colon tumor formation when administered following chemical carcinogen and induction of chronic colitis. AIM: This study seeks to better understand the effect of E2 on acute colitis in the presence and absence of estrogen receptor β (ERβ)...
June 1, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28572804/serum-cytokine-profiles-differentiating-hemorrhagic-fever-with-renal-syndrome-and-hantavirus-pulmonary-syndrome
#20
Svetlana F Khaiboullina, Silvana Levis, Sergey P Morzunov, Ekaterina V Martynova, Vladimir A Anokhin, Oleg A Gusev, Stephen C St Jeor, Vincent C Lombardi, Albert A Rizvanov
Hantavirus infection is an acute zoonosis that clinically manifests in two primary forms, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). HFRS is endemic in Europe and Russia, where the mild form of the disease is prevalent in the Tatarstan region. HPS is endemic in Argentina, as well as other countries of North and South American. HFRS and HPS are usually acquired via the upper respiratory tract by inhalation of virus-contaminated aerosol. Although the pathogenesis of HFRS and HPS remains largely unknown, postmortem tissue studies have identified endothelial cells as the primary target of infection...
2017: Frontiers in Immunology
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