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Multiplex and cytokine

Hanne A Eide, Ingerid Skjei Knudtsen, Vandana Sandhu, Ayca M Løndalen, Ann Rita Halvorsen, Azadeh Abravan, Elin H Kure, Trond V Bogsrud, Odd Terje Brustugun, Jon Amund Kyte, Eirik Malinen, Åslaug Helland
Purpose: Radiation therapy effectively kills cancer cells and elicits local effects in the irradiated tissue. The aim of this study was to investigate the kinetics of cytokines in the serum of patients with lung cancer undergoing radiation therapy and to identify associations with metabolic tumor burden as determined by 2-deoxy-2-fluoro-D-glucose (18 F-FDG) positron emission tomography (PET). Methods and materials: Forty-five patients with advanced non-small cell lung cancer were included in a phase 2 clinical trial and randomized between fractionated thoracic radiation therapy alone or concurrent with an epidermal growth factor receptor inhibitor...
April 2018: Advances in Radiation Oncology
Soledad Carinelli, Cristina Xufré, Mercè Martí, María Isabel Pividori
Interferon-γ is a proinflammatory cytokine, and its production is related with effective host defense against intracellular pathogens. Therefore, the level of interferon-γ is considered a good biomarker for intracellular infections. It is also useful for the assessment, treatment progression and follow-up of non-communicable diseases, including cancer and autoimmune disorders, among others. This work addresses the development of a novel interferon-γ release assay (IGRA) to evaluate the expression of interferon-γ transcripts as biomarker produced by isolated T cells, as a main advantage...
May 22, 2018: Biosensors & Bioelectronics
Sven H Loosen, Maximilian Schulze-Hagen, Catherine Leyh, Fabian Benz, Mihael Vucur, Christiane Kuhl, Christian Trautwein, Frank Tacke, Philipp Bruners, Christoph Roderburg, Tom Luedde
While surgical resection represents the standard potentially curative therapy for liver cancer, transarterial chemoembolization (TACE) has evolved as a standard therapy for intermediate-stage hepatocellular carcinoma (HCC) as well as liver metastases. However, it is still not fully understood which patients particularly benefit from TACE. Cytokines represent a broad category of signaling molecules that might reflect concomitant inflammation as an adverse prognostic factor. Here, we evaluated the role of interleukin (IL)-6, IL-8, and CC-chemokine ligand (CCL)22 as biomarkers in the context of TACE treatment...
June 14, 2018: International Journal of Molecular Sciences
Angel F Farinas, Ravinder Bamba, Alonda C Pollins, Nancy L Cardwell, Lillian B Nanney, Wesley P Thayer
BACKGROUND: Individuals in the geriatric age range are more prone than younger individuals to convert their partial thickness thermal burns into full thickness injuries. We hypothesized that this often observed clinical phenomenon is strongly related to differential local injury responses mediated by the immune system. MATERIALS & METHODS: Skin samples from areas with partial thickness thermal burns were obtained during routine excision and grafting procedures between post burn days 2-6...
June 9, 2018: Burns: Journal of the International Society for Burn Injuries
Gunnar Lewon Lutterkort, Anika Wranke, Julia Hengst, Cihan Yurdaydin, Judith Stift, Birgit Bremer, Svenja Hardtke, Onur Keskin, Ramazan Idilman, Michael P Manns, Hans Peter Dienes, Christine Falk, Heiner Wedemeyer, Benjamin Heidrich
Chronic hepatitis D is caused by coinfection of hepatitis B and hepatitis D virus. While HDV is the dominant virus over HBV in the majority of cases, mechanisms and consequences of viral dominances are largely unknown. We aimed to investigate associations between viral dominance patterns and patients' characteristics and inflammatory features. 109 HDV patients treated with PEG-IFNa-2a within the international multicentre, prospective HIDIT-2 trial were studied. Patients were classified as D- or B-dominant if the viral load of one virus exceeded that of the other virus by more than 1log10 ...
June 11, 2018: Journal of Viral Hepatitis
Shouquan Wu, Yu Wang, Miaomiao Zhang, Saurav S Shrestha, Minggui Wang, Jian-Qing He
Background: The factors that predispose to pulmonary tuberculosis (PTB) are not fully understood. Previous studies have shown that cytokine gene polymorphisms were associated with PTB. Objectives: In this study, we have investigated the relationship between ILB , IL6, and TNFα polymorphisms and a predisposition to Mycobacterium tuberculosis (MTB) infection and PTB. Methods: A total of 209 cases of PTB, 201 subjects with latent TB infection (LTBI), and 204 healthy controls (HCS) were included in this study...
2018: BioMed Research International
Young-Jin Kim, Ji Young Lee, Hyun-Ju Kim, Do-Hoon Kim, Tae Hee Lee, Mi Suk Kang, Wansu Park
The dry root of Angelica sinensis (Oliv.) Diels, also known as “female ginseng”, is a popular herbal drug amongst women, used to treat a variety of health issues and cardiovascular diseases. The aim of this study is to evaluate the detailed molecular mechanism for anti-inflammatory effects of Angelica sinensis root water extract (ASW). The anti-inflammatory effect of ASW on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages was evaluated by the tetrazolium-based colorimetric assay (MTT), Griess reagent assay, multiplex cytokine assay, real time reverse transcription polymerase chain reaction (RT-PCR), and Fluo-4 calcium assay...
May 21, 2018: Nutrients
Sabine Schröder, Dajana Juerß, Stephan Kriesen, Katrin Manda, Guido Hildebrandt
PURPOSE: The application of radiation therapy is not only used to treat cancer, in Germany it is also an accepted and empirically established treatment of patients with benign diseases at low doses. The immune modulatory response generated by low-dose radiation therapy has a supporting anti-inflammatory effect within the treatment of inflammation-related diseases. The aim of this study was to investigate the effect of ionizing radiation (IR) on the expression and secretion of inflammatory mediators by endothelial cells (EC) exposed to low and moderate doses...
June 8, 2018: International Journal of Radiation Biology
Justin H Turner, Ping Li, Rakesh K Chandra
BACKGROUND: Chronic rhinosinusitis (CRS) is a diverse clinical syndrome with a heterogeneous pathophysiology. Early attempts to identify CRS endotypes and biomarkers have largely relied on analysis of surgically obtained tissue, thus limiting their practical utility. This study examined the ability of mucus T helper 2 (Th2) biomarkers to predict CRS disease severity and clinical characteristics. METHODS: CRS (n = 90) and healthy control subjects (n = 17) were prospectively enrolled prior to surgical intervention and mucus levels of interleukin (IL)-4, IL-5, and IL-13 were determined using a multiplex cytometric bead assay...
June 8, 2018: International Forum of Allergy & Rhinology
Lorena Lam-Franco, Yocanxochitl Perfecto-Avalos, Beatriz E Patiño-Ramírez, Alejandro Rodríguez García
AIMS: The purpose of this paper was to determine the lacrimal concentration of IL-1α and MMP-9 in patients with active ocular rosacea before and after systemic treatment with azithromycin or doxycycline. METHODS: After 4 weeks of therapy with azithromycin (500 mg/day, 3 days a week PO) or doxycycline (200 mg/day PO), lacrimal samples were analyzed using an enzyme-linked immunosorbent assay multiplex. RESULTS: There was a significant difference between baseline IL-1α (37...
June 6, 2018: Ophthalmic Research
J A Jaffey, J Amorim, A E DeClue
Hypovitaminosis D has been identified as a predictor of mortality in human beings, dogs, cats and foals. However, the immunomodulatory effects of vitamin D in critically ill dogs has not been evaluated. The aim of this study was to evaluate the effect of calcitriol on cytokine production from whole blood collected from critically ill dogs in vitro. Twelve critically ill dogs admitted to a veterinary intensive care unit (ICU) were enrolled in a prospective cohort study. Whole blood from these dogs was incubated with calcitriol (2×10-7 M) or ethanol (control) for 24h...
June 2018: Veterinary Journal
Jennifer A Juno, Jillian L M Waruk, Kathleen M Wragg, Christine Mesa, Carmen Lopez, Joe Bueti, Stephen J Kent, T Blake Ball, Sandra A Kiazyk
Background: End-stage renal disease (ESRD) is associated with an increased susceptibility to infectious diseases, including infection with Mycobacterium tuberculosis (Mtb) . Mucosal-associated invariant T (MAIT) cells recognize vitamin B metabolites produced by many bacterial species, including Mtb, and may play an important role in providing protective immunity against tuberculosis infection in the lung. To date, little is known about MAIT cell frequency, phenotype, or function in ESRD patients...
2018: Frontiers in Immunology
Maha Ahmed Al-Mozaini, Anthony G Tsolaki, Munirah Abdul-Aziz, Suhair M Abozaid, Mohammed N Al-Ahdal, Ansar A Pathan, Valarmathy Murugaiah, Evgeny M Makarov, Anuvinder Kaur, Robert B Sim, Uday Kishore, Lubna Kouser
Mycobacterium tuberculosis can proficiently enter macrophages and diminish complement activation on its cell surface. Within macrophages, the mycobacterium can suppress macrophage apoptosis and survive within the intracellular environment. Previously, we have shown that complement regulatory proteins such as factor H may interfere with pathogen-macrophage interactions during tuberculosis infection. In this study, we show that Mycobacterium bovis BCG binds properdin, an upregulator of the complement alternative pathway...
2018: Frontiers in Immunology
E-B Hysing, L Smith, M Thulin, R Karlsten, T Gordh
Aims A few previous studies indicate an ongoing of low-grade systemic inflammation in chronic pain patients (CPP) [1, 2]. In the present study we investigated the plasma inflammatory profile in severely impaired chronic pain patients. In addition we studied if there were any alterations in inflammation patterns at one-year follow up, after the patients had taken part in a CBT-ACT based 4 weeks in-hospital pain rehabilitation program (PRP). Methods Blood samples were collected from 52 well characterized chronic pain patients...
December 29, 2017: Scandinavian Journal of Pain
Grzegorz Raba, Jacek Tabarkiewicz
Predicting preterm delivery within 7 days is very important for the proper timing of glucocorticosteroid administration. If within 7 days after glucocorticosteroid administration, the delivery does not occur, it remains questionable if repeated glucocorticosteroid therapy results in improved infant respiratory function. Therefore, differentiation of preterm delivery from false preterm delivery is clinically significant. The aim of this study was to create a diagnostic algorithm to distinguish preterm delivery from false preterm delivery on the basis of concentrations of selected cytokines...
2018: Journal of Immunology Research
Takahiro Hokari, Toshiya Morozumi, Yasutaka Komatsu, Taro Shimizu, Toshiaki Yoshino, Maki Tanaka, Yoshie Tanaka, Kaname Nohno, Takehiko Kubota, Hiromasa Yoshie
Objective: We evaluated the efficacies of antimicrobial photodynamic therapy (aPDT) and minocycline ointment (MO) on clinical and bacteriological markers and the local host inflammatory response. Materials and Methods: A total of 30 patients with chronic periodontitis were randomly assigned to two groups. Selected periodontal pockets (probing depth 5-7 mm with bleeding on probing) were treated with aPDT or MO. Measurements of clinical parameters and the collection of gingival crevicular fluid (GCF) and subgingival plaque were performed at baseline, and at 1 and 4 weeks after treatment...
2018: International Journal of Dentistry
Fathia G Jaat, Sajidah F Hasan, Audrey Perry, Sharon Cookson, Santosh Murali, John D Perry, Clare V Lanyon, Anthony De Soyza, Stephen M Todryk
BACKGROUND: As a way to determine markers of infection or disease informing disease management, and to reveal disease-associated immune mechanisms, this study sought to measure antibody and T cell responses against key lung pathogens and to relate these to patients' microbial colonization status, exacerbation history and lung function, in Bronchiectasis (BR) and Chronic Obstructive Pulmonary Disease (COPD). METHODS: One hundred nineteen patients with stable BR, 58 with COPD and 28 healthy volunteers were recruited and spirometry was performed...
May 30, 2018: Respiratory Research
Viythia Katharesan, Shane Deery, Ian P Johnson
Increases in inflammatory cytokines are reported to have both neuroprotective and neurotoxic effects depending on the type and age of neurones studied. This study aimed to determine the effect of experimental inflammation induced by Lipopolysaccharide (LPS) on the survival of injured male adult rat facial motoneurones. Time- and dose- response studies were done to optimise the LPS administration time and dose, to best correlate with inflammatory levels previously reported for aged rats. 12 cytokines were assayed through multiplex analysis...
May 26, 2018: Brain Research
Yiyi Yang, Antonio Boza-Serrano, Christopher J R Dunning, Bettina Hjelm Clausen, Kate Lykke Lambertsen, Tomas Deierborg
BACKGROUND: Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS). Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication. However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain. Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content...
May 28, 2018: Journal of Neuroinflammation
C R Bain, D F Draxler, R Taylor, S Wallace, O Gouldthorpe, T B Corcoran, P S Myles, R L Medcalf, K Bozaoglu
Dexamethasone is often administered to surgical patients for anti-emetic prophylaxis. This study examined the early (up to 24 h) in-vivo effects of dexamethasone (8 mg) to demonstrate the magnitude and temporal nature of changes on circulating peripheral blood mononuclear cell gene expression and activation in 10 healthy male volunteers. Blood samples were drawn at baseline, 2 h, 4 h and 24 h. Gene expression was measured using quantitative real-time polymerase chain reaction. Cytokine expression was measured using multiplex immuno-assays...
May 28, 2018: Anaesthesia
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