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Multiplex and cytokine

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https://www.readbyqxmd.com/read/29675026/multiplex-cytokine-analysis-of-aqueous-humor-in-juvenile-idiopathic-arthritis-associated-anterior-uveitis-with-or-without-secondary-glaucoma
#1
Dirk Bauer, Maren Kasper, Karoline Walscheid, Jörg M Koch, Philipp S Müther, Bernd Kirchhof, Arnd Heiligenhaus, Carsten Heinz
Patients with juvenile idiopathic arthritis often develop chronic anterior uveitis (JIAU). JIAU patients possess a particularly high risk for developing secondary glaucoma when inflammatory inactivity has been achieved. By using multiplex bead assay analysis, we assessed levels of pro- and anti-inflammatory cytokines, chemokines, or metalloproteinases in the aqueous humor (AH) of patients with clinically inactive JIAU with (JIAUwG) or without secondary glaucoma (JIAUwoG), or from patients with senile cataract as controls...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29673332/expression-of-cytokines-in-aqueous-humor-from-fungal-keratitis-patients
#2
Yingnan Zhang, Qingfeng Liang, Yang Liu, Zhiqiang Pan, Christophe Baudouin, Antoine Labbé, Qingxian Lu
BACKGROUND: Although a series of reports on corneal fungal infection have been published, studies on pathogenic mechanisms and inflammation-associated cytokines remain limited. In this study, aqueous humor samples from fungal keratitis patients were collected to examine cytokine patterns and cellular profile for the pathogenesis of fungal keratitis. METHODS: The aqueous humor samples were collected from ten patients with advanced stage fungal keratitis. Eight aqueous humor samples from patients with keratoconus or corneal dystrophy were taken as control...
April 19, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29672707/longitudinal-study-of-cellular-and-systemic-cytokines-signatures-define-the-dynamics-of-a-balanced-immune-environment-in-disease-manifestation-in-zika-virus-infected-patients
#3
Fok-Moon Lum, David C B Lye, Jeslin J L Tan, Bernett Lee, Po-Ying Chia, Tze-Kwang Chua, Siti N Amrun, Yiu-Wing Kam, Wearn-Xin Yee, Wei-Ping Ling, Vanessa W X Lim, Vincent J X Pang, Linda K Lee, Esther W H Mok, Chia-Yin Chong, Yee-Sin Leo, Lisa F P Ng
Background: The unexpected re-emergence of Zika virus (ZIKV) has caused numerous outbreaks globally. This study characterized the host immune responses during ZIKV infection. Methods: Patient samples were collected longitudinally during the acute, convalescence and recovery phases of ZIKV infection over 6 months during the Singapore outbreak in late 2016. Plasma immune mediators were profiled via multiplex micro-bead assay, while changes in blood cell numbers were determined with immune-phenotyping...
April 16, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29665817/elevated-il-17-levels-in-semi-immune-anaemic-mice-infected-with-plasmodium-berghei-anka
#4
Gideon Kofi Helegbe, Nguyen Tien Huy, Tetsuo Yanagi, Mohammed Nasir Shuaibu, Mihoko Kikuchi, Mahamoud Sama Cherif, Kenji Hirayama
BACKGROUND: Alterations in inflammatory cytokines and genetic background of the host contribute to the outcome of malaria infection. Despite the promising protective role of IL-17 in infections, little attention is given to further understand its importance in the pathogenesis of severe malaria anaemia in chronic/endemic situations. The objective of this study, therefore, was to evaluate IL-17 levels in anaemic condition and its association with host genetic factors. METHODS: Two mice strains (Balb/c and CBA) were crossed to get the F1 progeny, and were (F1, Balb/c, CBA) taken through 6 cycles of Plasmodium berghei (ANKA strain) infection and chloroquine/pyrimethamine treatment to generate semi-immune status...
April 17, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29660943/circulating-cell-free-micro-rna-profiles-reflect-discordant-development-of-dementia-in-monozygotic-twins
#5
Jonas Mengel-From, Mette E Rønne, Anting L Carlsen, Kristin Skogstrand, Lisbeth A Larsen, Qihua Tan, Lene Christiansen, Kaare Christensen, Niels H H Heegaard
We aim to examine if circulating micro-RNA and cytokine levels associate with dementia diagnosis and cognitive scores. To test our hypothesis, we use plasma donated from 48 monozygotic twin pairs in 1997 and 46 micro-RNAs and 10 cytokines were quantified using microfluidic RT-qPCR and multiplex solid-phase immunoassays, respectively. Micro-RNA and cytokine profiling were examined for associations with dementia diagnoses in a longitudinal registry study or with cognitive scores at baseline. Thirty-six micro-RNAs and all cytokines were detected consistently...
April 11, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29657145/hla-drb1-shared-epitope-alleles-and-disease-activity-are-correlated-with-reduced-t-cell-receptor-repertoire-diversity-in-cd4-t-cells-in-rheumatoid-arthritis
#6
Keiichi Sakurai, Kazuyoshi Ishigaki, Hirofumi Shoda, Yasuo Nagafuchi, Yumi Tsuchida, Shuji Sumitomo, Hiroko Kanda, Akari Suzuki, Yuta Kochi, Kazuhiko Yamamoto, Keishi Fujio
OBJECTIVE: Shared epitope (SE) alleles are the most significant genetic susceptibility locus in rheumatoid arthritis (RA); however, their target populations in CD4+ T cells are not well elucidated. We analyzed the association between SE alleles and the T cell receptor (TCR) repertoire diversity of naive and memory CD4+ T cells using next-generation sequencing (NGS). METHODS: The TCR beta chains in naive and memory CD4+ T cells from the peripheral blood of 22 patients with RA and 18 age- and sex-matched healthy donors (HD) were analyzed by NGS...
April 15, 2018: Journal of Rheumatology
https://www.readbyqxmd.com/read/29642398/novel-nanoparticulate-and-ionic-titanium-antigens-for-hypersensitivity-testing
#7
Paul Johan Høl, Einar K Kristoffersen, Nils Roar Gjerdet, Amanda S Pellowe
Titanium is used in a wide variety of materials ranging from medical devices to materials used in everyday life. Adverse biological reactions that could occur in patients, consumers, and workers should be monitored and prevented. There is a lack of available agents to test and predict titanium-related hypersensitivity. The aim of this study was to develop two bioavailable titanium substances in ionic and nanoparticulate form to serve as antigens for hypersensitivity testing in vitro. Peripheral blood mononuclear cells from 20 test subjects were stimulated with the antigens and secretion of monocytic and lymphatic cytokines and chemokines were measured by a multiplex bead assay...
April 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29630031/elevated-cell-free-mitochondrial-dna-in-filtered-plasma-is-associated-with-hiv-infection-and-inflammation
#8
Omair Arshad, Izabella Gadawska, Beheroze Sattha, Hélène C F Côté, Anthony Y Y Hsieh
BACKGROUND: Increased cell-free DNA levels are associated with poor health outcomes, and cell-free mitochondrial DNA (cf-mtDNA) has proinflammatory properties. Given that HIV infection is associated with chronic inflammation, we investigated the relationship between cf-mtDNA and proinflammatory cytokine interleukin-6 (IL-6) in the context of HIV infection. We also optimized separation of cell-free plasma from blood. SETTING: In this retrospective cross-sectional study, we collected blood, demographic information, and clinical data from 99 HIV-infected and 103 HIV-uninfected adults and children enrolled in the Children and Women: AntiRetrovirals and Markers of Aging pan-Canadian (CARMA) cohort...
May 1, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29625462/retinal-pigment-epithelial-cells-control-early-mycobacterium-tuberculosis-infection-via-interferon-signaling
#9
Rina La Distia Nora, Kimberley V Walburg, P Martin van Hagen, Sigrid M A Swagemakers, Peter J van der Spek, Edwin Quinten, Mirjam van Velthoven, Tom H M Ottenhoff, Willem A Dik, Mariëlle C Haks
Purpose: Mycobacterium tuberculosis (Mtb) bacilli have been found in retinal pigment epithelial (RPE) cells from uveitis patients without signs of systemic tuberculosis (TB) infection. RPE cells are important for ocular immune privilege and uveitis development. Methods: To address a potential role for Mtb-infected RPE cells in the development of uveitis, we delineated the response to Mtb infection in human RPE cells and primary human macrophages, the main target cell of Mtb...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29625077/optimising-the-collection-of-female-genital-tract-fluid-for-cytokine-analysis-in-pregnant-women
#10
C S Short, R Quinlan, P Bennett, R J Shattock, G P Taylor
INTRODUCTION: To better understand the immunology of pregnancy, study of female genital tract fluid (FGF) is desirable. However the optimum method of collection of FGF in pregnant women for immunological methods, specifically cytokine measurement, is unknown. METHODS: A prospective study of HIV-uninfected pregnant women comparing two methods of FGF collection: polyvinyl acetal sponge collection of cervical fluid (CF) and menstrual cup collection of cervicovaginal fluid (CVF)...
April 3, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29617399/activation-of-human-macrophages-by-human-corneal-allogen-in-vitro
#11
Paola Kammrath Betancor, Antonia Hildebrand, Daniel Böhringer, Florian Emmerich, Günther Schlunck, Thomas Reinhard, Thabo Lapp
PURPOSE: To study distinct aspects of human monocyte-derived macrophage (MDM) activation by human corneal tissue as a possible initial stage in human corneal allograft rejection. METHODS: Human monocytes were isolated from peripheral blood mononuclear cells (PBMC) and differentiated into MDM. Human corneas with or without endothelium were fragmented using a standardized protocol. MDM were stimulated with human corneal fragments, corneal fragment supernatant, lipopolysaccharide (LPS) or interferon-gamma (IFNγ), and expression profiles for 34 cytokines were determined in MDM-conditioned media using a Luminex bead-based multiplex assay...
2018: PloS One
https://www.readbyqxmd.com/read/29614267/influence-of-delayed-sample-processing-on-blood-immune-cell-phenotypes-immune-cell-responses-and-serum-anti-influenza-vaccine-antibody-titres
#12
Vivian Castro-Herrera, Mark Lown, George Lewith, Elizabeth A Miles, Philip C Calder
Provision of blood from distant research partners to a central laboratory can result in delayed blood processing prior to assessment of immune parameters. It is important to evaluate the effect of such delays on immune parameters. This study investigated the effect of storage of blood at room temperature for up to 72 h prior to processing and analysis on a range of immune parameters. Blood was collected from 10 healthy participants and analysed immediately (day 0) or after storage at room temperature for 24, 48 or 72 h (days 1, 2 and 3)...
March 31, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29611100/resveratrol-acts-anti-inflammatory-and-neuroprotective-in-an-infant-rat-model-of-pneumococcal-meningitis-by-modulating-the-hippocampal-mirnome
#13
Karina Barbosa de Queiroz, Thaís Dos Santos Fontes Pereira, Márcio Sobreira Silva Araújo, Ricardo Santiago Gomez, Roney Santos Coimbra
Resveratrol (RSV) is anti-inflammatory and neuroprotective, cross the blood-brain barrier (BBB) and has a safe profile. Besides, RSV modulates the expression of some miRNAs related to neurological disorders. Thus, we hypothesized that RSV can be neuroprotective in pneumococcal meningitis by modulating the global microRNA expression profile (miRNome). Eleven-day old rats were intracysternally infected with S. pneumoniae (~ 2 × 106 c.f.u.) and were orally administered with RSV (50 mg/kg) or vehicle in pre-treatment (before infection) or post-treatment schedules (3 and 18 h p...
April 2, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29609271/-killing-effect-of-robo1-targeted-chimeric-antigen-receptor-modified-nk92-cells-against-glioma-and-neuroblastoma-cells
#14
Y Qu, J Z Bi
Objective: To study the cytotoxicity of Robo1-CAR-NK92 cells against U87-MG and SH-SY5Y cells, to explore the effects of IL-15, IL-21 and dexamethasone on the proliferation, survival and cytotoxicity of Robo1-CAR-NK92 cells and to optimize the culture protocol. Methods: Robo1-CAR-NK92 cells were constructed by lentivirus transfection.The Robo1 car positive cells were sorted, expanded and detected by flow cytometry.The levels of Robo1 expression in SH-SY5Y and U87-MG cells were examined by flow cytometry.The cytotoxicity of Robo1-CAR-NK92 or NK92 cells against target cells was tested by CCK-8 and live cell imaging...
March 20, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29603385/downregulated-eosinophil-activity-in-ulcerative-colitis-with-concomitant-primary-sclerosing-cholangitis
#15
Maria Lampinen, Annika Fredricsson, Johan Vessby, Johana Fernandez Martinez, Alkwin Wanders, Fredrik Rorsman, Marie Carlson
Primary sclerosing cholangitis (PSC) is a chronic bile duct inflammation strongly connected to ulcerative colitis (UC). PSC is associated with an increased risk of colon cancer, but the link between the intestinal and the bile duct inflammation is still unknown. Also, the involvement of intestinal immune cells in the pathogenesis of PSC remains to be determined. The eosinophil granulocyte is one of the immune cells implicated in the inflammatory process of ulcerative colitis. This study was performed to determine how the accumulation and activation of intestinal eosinophils may differ between UC with and without concomitant PSC, and how this may be influenced by the cytokine/chemokine profile of the intestinal compartment...
March 30, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29600943/silent-arterial-inflammation-during-the-apparent-remission-state-of-takayasu-s-arteritis-what-do-cytokines-tell-us
#16
Bruna Savioli, Bruno Ramos Salu, Marlon Vilela de Brito, Maria Luiza Vilela Oliva, Alexandre W S de Souza
OBJECTIVES: To evaluate serum cytokines as biomarkers of smoldering disease activity in patients with Takayasu's arteritis (TAK) in remission. METHODS: Thirty-four TAK patients with stable disease during the last 6 months and 22 healthy controls (HC) were included in a cross-sectional study. Serum levels of pro-inflammatory, anti-inflammatory, Th1, Th2, Th9, Th17 and Th22 cytokines were measured by the multiplex technique. RESULTS: No significant differences regarding serum cytokine levels were found between TAK patients and HC...
March 19, 2018: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29594209/assessment-of-pulmonary-18-f-fdg-pet-uptake-and-cytokine-profiles-in-non-small-cell-lung-cancer-patients-treated-with-radiotherapy-and-erlotinib
#17
Azadeh Abravan, Hanne Astrid Eide, Ingerid Skjei Knudtsen, Ayca Muftuler Løndalen, Åslaug Helland, Eirik Malinen
Purpose: To investigate effects of radiotherapy (RT) and erlotinib on pulmonary glucose uptake using 2-deoxy-2-(18F)fluoro-D-glucose (18 F-FDG) positron emission tomography (PET) during and after treatment of non-small cell lung cancer (NSCLC) and to identify associations between serum cytokine levels and lung glucose uptake. Material and methods: Twenty-seven patients with advanced NSCLC, receiving RT alone or concomitant RT and erlotinib therapy, were examined by18 F-FDG PET before, during, and after treatment...
June 2017: Clinical and Translational Radiation Oncology
https://www.readbyqxmd.com/read/29587383/protein-expression-in-tonsillar-and-base-of-tongue-cancer-and-in-relation-to-human-papillomavirus-hpv-and-clinical-outcome
#18
Torbjörn Ramqvist, Anders Näsman, Bo Franzén, Cinzia Bersani, Andrey Alexeyenko, Susanne Becker, Linnea Haeggblom, Aeneas Kolev, Tina Dalianis, Eva Munck-Wikland
Human papillomavirus (HPV) is a major etiological factor for tonsillar and the base of tongue cancer (TSCC/BOTSCC). HPV-positive and HPV-negative TSCC/BOTSCC present major differences in mutations, mRNA expression and clinical outcome. Earlier protein studies on TSCC/BOTSCC have mainly analyzed individual proteins. Here, the aim was to compare a larger set of cancer and immune related proteins in HPV-positive and HPV-negative TSCC/BOTSCC in relation to normal tissue, presence of HPV, and clinical outcome. Fresh frozen tissue from 42 HPV-positive and 17 HPV-negative TSCC/BOTSCC, and corresponding normal samples, were analyzed for expression of 167 proteins using two Olink multiplex immunoassays...
March 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29579271/interleukin-34-is-present-at-the-fetal-maternal-interface-and-induces-immunoregulatory-macrophages-of-a-decidual-phenotype-in-vitro
#19
R Lindau, R B Mehta, G E Lash, G Papapavlou, R Boij, G Berg, M C Jenmalm, J Ernerudh, J Svensson-Arvelund
STUDY QUESTION: Is the newly discovered cytokine interleukin (IL)-34 expressed at the human fetal-maternal interface in order to influence polarization of monocytes into macrophages of a decidual immunoregulatory phenotype? SUMMARY ANSWER: IL-34 was found to be present at the fetal-maternal interface, in both fetal placenta and maternal decidua, and it was able to polarize monocytes into macrophages of a decidual phenotype. WHAT IS KNOWN ALREADY: IL-34 was shown to bind to the same receptor as macrophage-colony stimulating factor (M-CSF), which has an important immunomodulatory role at the fetal-maternal interface, for example by polarizing decidual macrophages to an M2-like regulatory phenotype...
April 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29552179/prognostic-significance-of-copy-number-alterations-detected-by-multi-link-probe-amplification-of-multiple-genes-in-adult-acute-lymphoblastic-leukemia
#20
Qiuyun Fang, Tian Yuan, Yan Li, Juan Feng, Xiaoyuan Gong, Qinghua Li, Xingli Zhao, Kaiqi Liu, Kejing Tang, Zheng Tian, Qi Zhang, Ying Wang, Bingcheng Liu, Min Wang, Kun Ru, Jianxiang Wang, Yingchang Mi
The multiplex ligation-dependent probe amplification (MLPA) method was used to detect the copy number alterations (CNAs) of IKAROS family zinc finger 1 ( IKZF1 ), paired box 5 ( PAX5 ), ETS variant 6 ( ETV6 ), RB transcriptional corepressor 1 ( RB1 ), BTG anti-proliferation factor 1 ( BTG1 ), early B-cell factor 1 ( EBF1 ), cyclin dependent kinase inhibitor 2A/2B ( CDKN2A/2B ) and cytokine receptor like factor 2 ( CRLF2 ) genes in 87 adults with acute lymphoblastic leukemia (ALL) in China. The effects of CNAs on prognosis were analyzed...
April 2018: Oncology Letters
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