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https://www.readbyqxmd.com/read/27914890/biomass-and-oil-production-by-chlorella-vulgaris-and-four-other-microalgae-effects-of-salinity-and-other-factors
#1
Tiyaporn Luangpipat, Yusuf Chisti
Five nominally freshwater microalgae (Chlorella vulgaris, Choricystis minor, Neochloris sp., Pseudococcomyxa simplex, Scenedesmus sp.) with a known ability to produce high-levels of lipids for possible use as fuel oils were evaluated for their ability to thrive and produce lipids in seawater and brackish water. Only C. vulgaris was found to thrive and produce lipids in full strength seawater. Seawater tolerant strains of C. vulgaris are unusual. Lipid productivity in nutrient sufficient seawater exceeded 37mgL(-1)d(-1) and was nearly 2-fold greater than in freshwater...
November 30, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27914260/the-extent-of-fermentative-transformation-of-phenolic-compounds-in-the-bioanode-controls-exoelectrogenic-activity-in-a-microbial-electrolysis-cell
#2
Xiaofei Zeng, Maya A Collins, Abhijeet P Borole, Spyros G Pavlostathis
Phenolic compounds in hydrolysate/pyrolysate and wastewater streams produced during the pretreatment of lignocellulosic biomass for biofuel production present a significant challenge in downstream processes. Bioelectrochemical systems are increasingly recognized as an alternative technology to handle biomass-derived streams and to promote water reuse in biofuel production. Thus, a thorough understanding of the fate of phenolic compounds in bioanodes is urgently needed. The present study investigated the biotransformation of three structurally similar phenolic compounds (syringic acid, SA; vanillic acid, VA; 4-hydroxybenzoic acid, HBA), and their individual contribution to exoelectrogenesis in a microbial electrolysis cell (MEC) bioanode...
November 27, 2016: Water Research
https://www.readbyqxmd.com/read/27913828/engineering-and-evolution-of-saccharomyces-cerevisiae-to-produce-biofuels-and-chemicals
#3
Timothy L Turner, Heejin Kim, In Iok Kong, Jing-Jing Liu, Guo-Chang Zhang, Yong-Su Jin
To mitigate global climate change caused partly by the use of fossil fuels, the production of fuels and chemicals from renewable biomass has been attempted. The conversion of various sugars from renewable biomass into biofuels by engineered baker's yeast (Saccharomyces cerevisiae) is one major direction which has grown dramatically in recent years. As well as shifting away from fossil fuels, the production of commodity chemicals by engineered S. cerevisiae has also increased significantly. The traditional approaches of biochemical and metabolic engineering to develop economic bioconversion processes in laboratory and industrial settings have been accelerated by rapid advancements in the areas of yeast genomics, synthetic biology, and systems biology...
December 3, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27913282/proteomics-provides-insights-into-biological-pathways-altered-by-plant-growth-promoting-bacteria-and-arbuscular-mycorrhiza-in-sorghum-grown-in-marginal-soil
#4
Faten Dhawi, Rupali Datta, Wusirika Ramakrishna
Sorghum is an economically important crop, a model system for gene discovery and a biofuel source. Sorghum seedlings were subjected to three microbial treatments, plant growth promoting bacteria (B), arbuscular mycorrhizal (AM) fungi mix with two Glomus species (G. aggregatum and G. etunicatum), Funelliformis mosseae and Rhizophagus irregularis (My), and B and My combined (My+B). Proteomic analysis was conducted followed by integration with metabolite, plant biomass and nutrient data. Out of 366 differentially expressed proteins in sorghum roots, 44 upregulated proteins overlapping among three treatment groups showed positive correlation with sorghum biomass or element uptake or both...
November 29, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27912184/substrate-dependent-modulation-of-butanol-to-ethanol-ratio-in-non-acetone-forming-clostridium-sporogenes-ncim-2918
#5
Mehak Kaushal, Saumya Ahlawat, Mayurketan Mukherjee, Muthusivaramapandian Muthuraj, Gargi Goswami, Debasish Das
Present study reports a non-acetone producing Clostridium sporogenes strain as a potential producer of liquid biofuels. Alcohol production was positively regulated by sorbitol and instant dry yeast as carbon and nitrogen sources respectively. Media optimization resulted in maximum butanol and ethanol titer (gL(-1)) of 12.1 and 7.9 respectively. Depending on the combination of carbon sources, the organism was found to manipulate its metabolism towards synthesis of either ethanol or butanol, thereby affecting the total alcohol titer...
November 16, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27911807/enhanced-limonene-production-in-cyanobacteria-reveals-photosynthesis-limitations
#6
Xin Wang, Wei Liu, Changpeng Xin, Yi Zheng, Yanbing Cheng, Su Sun, Runze Li, Xin-Guang Zhu, Susie Y Dai, Peter M Rentzepis, Joshua S Yuan
Terpenes are the major secondary metabolites produced by plants, and have diverse industrial applications as pharmaceuticals, fragrance, solvents, and biofuels. Cyanobacteria are equipped with efficient carbon fixation mechanism, and are ideal cell factories to produce various fuel and chemical products. Past efforts to produce terpenes in photosynthetic organisms have gained only limited success. Here we engineered the cyanobacterium Synechococcus elongatus PCC 7942 to efficiently produce limonene through modeling guided study...
November 23, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27910256/simultaneous-upgrading-of-furanics-and-phenolics-via-hydroxyalkylation-aldol-condensation-reactions
#7
Tuong V Bui, Tawan Sooknoi, Daniel E Resasco
The simultaneous conversion of cyclopentanone and m-cresol has been investigated on a series of solid acid catalysts. Both compounds are representative of biomass-derived streams. Cyclopentanone can be readily obtained from sugar-derived furfurals via Piancatelli rearrangement under reducing conditions. Cresol represents the family of phenolics, typically obtained from the depolymerization of lignin. In the first biomass conversion strategy proposed here, furfural is converted in high yields and selectivity to cyclopentanone (CPO) over metal catalysts such as Pd-Fe/SiO2 catalyst at 600 psia H2 and 150oC...
December 1, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27905924/current-knowledge-and-perspectives-of-paenibacillus-a-review
#8
REVIEW
Elliot Nicholas Grady, Jacqueline MacDonald, Linda Liu, Alex Richman, Ze-Chun Yuan
Isolated from a wide range of sources, the genus Paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Many Paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole-3-acetic acid (IAA), and release of siderophores that enable iron acquisition. They can also offer protection against insect herbivores and phytopathogens, including bacteria, fungi, nematodes, and viruses...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27905534/pore-scale-dynamics-of-enzyme-adsorption-swelling-and-reactive-dissolution-determine-sugar-yield-in-hemicellulose-hydrolysis-for-biofuel-production
#9
Sajal Kanti Dutta, Saikat Chakraborty
Hemicelluloses are the earth's second most abundant structural polymers, found in lignocellulosic biomass. Efficient enzymatic depolymerization of xylans by cleaving their β-(1 → 4)-glycosidic bonds to produce soluble sugars is instrumental to the cost-effective production of liquid biofuels. Here we show that the multi-scale two-phase process of enzymatic hydrolysis of amorphous hemicelluloses is dominated by its smallest scale-the pores. In the crucial first five hours, two to fourfold swelling of the xylan particles allow the enzymes to enter the pores and undergo rapid non-equilibrium adsorption on the pore surface before they hydrolyze the solid polymers, albeit non-competitively inhibited by the products xylose and xylobiose...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27903654/carbon-and-acyl-chain-flux-during-stress-induced-triglyceride-accumulation-by-stable-isotopic-labeling-of-the-polar-microalga-coccomyxa-subellipsoidea-c169
#10
James W Allen, Concetta C DiRusso, Paul N Black
Deriving biofuels and other lipoid products from algae is a promising future technology directly addressing global issues of atmospheric CO2 balance. To better understand the metabolism of triglyceride synthesis in algae, we examined their metabolic origins in the model species, Coccomyxa subellipsoidea C169, using stable isotopic labeling. Labeling patterns arising from (13)C-U-glucose, (13)CO2, or D2O supplementation were analyzed by GC-MS and/or LC-MS over time courses during nitrogen (N) starvation to address the roles of catabolic carbon (C) recycling, acyl chain redistribution, and de novo fatty acid (FA) synthesis during the expansion of the lipid bodies...
November 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27900666/improved-production-of-thermostable-cellulase-from-thermoascus-aurantiacus-rckk-by-fermentation-bioprocessing-and-its-application-in-the-hydrolysis-of-office-waste-paper-algal-pulp-and-biologically-treated-wheat-straw
#11
Kavish Kumar Jain, Sandeep Kumar, Deepa Deswal, Ramesh Chander Kuhad
Thermostable cellulases have wide variety of applications and distinctive advantages, but their low titer becomes the hurdle in their commercialization. In the present work, an assessment of optimum levels of significant factors (temperature, moisture ratio, inoculum size, and ammonium sulfate) and the effect of their interactions on production of thermostable CMCase, FPase, and β-glucosidase by Thermoascus aurantiacus RCKK under solid-state fermentation (SSF) was carried out using central composite design (CCD) of response surface methodology (RSM)...
November 30, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27898315/single-step-single-organism-bioethanol-production-and-bioconversion-of-lignocellulose-waste-materials-by-phlebioid-fungal-species
#12
Hans Mattila, Jaana Kuuskeri, Taina Lundell
Ethanol production from non-pretreated lignocellulose was carried out in a consolidated bioprocess with wood-decay fungi of phlebioid Polyporales. Ethanol production was attempted on glucose, spruce wood sawdust and waste core board. Substantial quantities of ethanol were achieved, and isolate Phlebia radiata 0043 produced 5.9g/L of ethanol reaching the yield of 10.4% ethanol from core board lignocellulose substrate. Acidic initial culture conditions (pH 3) induced ethanol fermentation compared to the more neutral environment...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27895709/the-dilemma-for-lipid-productivity-in-green-microalgae-importance-of-substrate-provision-in-improving-oil-yield-without-sacrificing-growth
#13
REVIEW
Kenneth Wei Min Tan, Yuan Kun Lee
Rising oil prices and concerns over climate change have resulted in more emphasis on research into renewable biofuels from microalgae. Unlike plants, green microalgae have higher biomass productivity, will not compete with food and agriculture, and do not require fertile land for cultivation. However, microalgae biofuels currently suffer from high capital and operating costs due to low yields and costly extraction methods. Microalgae grown under optimal conditions produce large amounts of biomass but with low neutral lipid content, while microalgae grown in nutrient starvation accumulate high levels of neutral lipids but are slow growing...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27895707/fungal-fermentation-on-anaerobic-digestate-for-lipid-based-biofuel-production
#14
Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao
BACKGROUND: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27895705/improved-sugar-yields-from-biomass-sorghum-feedstocks-comparing-low-lignin-mutants-and-pretreatment-chemistries
#15
Bruno Godin, Nick Nagle, Scott Sattler, Richard Agneessens, Jérôme Delcarte, Edward Wolfrum
BACKGROUND: For biofuel production processes to be economically efficient, it is essential to maximize the production of monomeric carbohydrates from the structural carbohydrates of feedstocks. One strategy for maximizing carbohydrate production is to identify less recalcitrant feedstock cultivars by performing some type of experimental screening on a large and diverse set of candidate materials, or by identifying genetic modifications (random or directed mutations or transgenic plants) that provide decreased recalcitrance...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27895286/pyrolysis-of-poppy-capsule-pulp-for-bio-oil-production
#16
Derya Yeşim Hopa, Nazan Yılmaz, Oğuzhan Alagöz, Meltem Dilek, Ahmet Helvacı, Ümit Durupınar
The feasibility of biofuel production via the pyrolysis of poppy capsule pulp, the main waste product of Afyon Alkoloid Factory, was investigated. The poppy capsule pulp was shown to have a high volatile matter content (ca. 76%). Pyrolysis experiments were carried out in the temperature range 400-550°C (heating rate 18°C min(-1) and holding time 20 min) under a nitrogen atmosphere. The chemical components of the bio-oil were characterized by Fourier transform infrared spectrometry and gas chromatography-mass spectrometry...
December 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27894803/evaluating-the-impacts-of-the-clean-cities-program
#17
Shiyong Qiu, Nikhil Kaza
The Department of Energy's Clean Cities program was created in 1993 to reduce petroleum usage in the transportation sector. The program promotes alternative fuels such as biofuels and fuel-saving strategies such as idle reduction and fleet management through coalitions of local government, non-profit, and private actors. Few studies have evaluated the impact of the program because of its complexity that include interrelated strategies of grants, education and training and diversity of participants. This paper uses a Difference-in-Differences (DiD) approach to evaluate the effectiveness of the program between 1990 and 2010...
November 25, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27891177/furfural-tolerance-and-detoxification-mechanism-in-candida-tropicalis
#18
Shizeng Wang, Gang Cheng, Chijioke Joshua, Zijun He, Xinxiao Sun, Ruimin Li, Lexuan Liu, Qipeng Yuan
BACKGROUND: Current biomass pretreatment by hydrothermal treatment (including acid hydrolysis, steam explosion, and high-temperature steaming) and ionic liquids generally generate inhibitors to the following fermentation process. Furfural is one of the typical inhibitors generated in hydrothermal treatment of biomass. Furfural could inhibit cell growth rate and decrease biofuel productivity of microbes. Candida tropicalis is a promising microbe for the production of biofuels and value-added chemicals using hemicellulose hydrolysate as carbon source...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27889523/identification-of-target-genes-and-processes-involved-in-erucic-acid-accumulation-during-seed-development-in-the-biodiesel-feedstock-pennycress-thlaspi-arvense-l
#19
Ana Claver, Raquel Rey, M Victoria López, Rafael Picorel, Miguel Alfonso
We studied erucic acid accumulation in the biodiesel feedstock Pennycress (Thlaspi arvense L.) as a first step towards the development of a sustainable strategy for biofuel production in the EU territory. To that end, two inbred Pennycress lines of European origin, "NASC" and "French," were cultivated in a controlled chamber and in experimental field plots, and their growth, seed production and seed oil characteristics analyzed. Differences in some agronomical traits like vernalization (winter-French versus spring-NASC), flowering time (delayed in the French line) and seed production (higher in the French line) were detected...
November 18, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27889478/sequential-enzymatic-saccharification-and-fermentation-of-ionic-liquid-and-organosolv-pretreated-agave-bagasse-for-ethanol-production
#20
Jose A Pérez-Pimienta, Alejandra Vargas-Tah, Karla M López-Ortega, Yessenia N Medina-López, Jorge A Mendoza-Pérez, Sayeny Avila, Seema Singh, Blake A Simmons, Inés Loaces, Alfredo Martinez
Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass...
November 16, 2016: Bioresource Technology
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