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https://www.readbyqxmd.com/read/28525799/thermophilic-moorella-thermoautotrophica-immobilized-cathode-enhanced-microbial-electrosynthesis-of-acetate-and-formate-from-co2
#1
Linpeng Yu, Yong Yuan, Jiahuan Tang, Shungui Zhou
Microbial electrosynthesis (MES) is a promising technique that converts electricity and CO2 to biofuels using microbes as the catalysts. However, most of previous MES are conducted at mesophilic temperatures and challenged by low performances. Here we report a significant electrosynthesis performance enhancement via immobilization of a thermophilic microbe to cathodes. A temperature-dependent electron uptake rate of Moorella thermoautotrophica was observed at a cathode potential of -0.4V (vs. SHE), with a maximum current density of 63...
May 13, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28521231/development-of-an-x-shape-airlift-photobioreactor-for-increasing-algal-biomass-and-biodiesel-production
#2
Hoang-Minh Pham, Ho Seok Kwak, Min-Eui Hong, Jeewon Lee, Won Seok Chang, Sang Jun Sim
The aim of this work was to develop a high efficient photobioreactor for increasing biomass and lipid production in microalgae by assessment of the hydrodynamic properties and kLa which are important parameters for improving the algal cultivation efficiency. We designed three different photobioreactors (H-Shape, X-Shape and serial-column). Among them, X-Shape showed the highest hydrodynamic properties and kLa for algal cultivation. Thus, we evaluated the biomass and the lipid production in a 20L scale-up X-Shape photobioreactor...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521157/use-of-experimentally-determined-henry-s-law-and-salting-out-constants-for-ethanol-in-seawater-for-determination-of-the-saturation-state-of-ethanol-in-coastal-waters
#3
Joan D Willey, Jacqueline P Powell, G Brooks Avery, Robert J Kieber, Ralph N Mead
The Henry's law constant for ethanol in seawater was experimentally determined to be 221 ± 4 M/atm at 22 °C compared with 247 ± 6 M/atm in pure water. The salting out coefficient for ethanol was 0.13 M(-1). In seawater ln(KH) = -(12.8 ± 0.7) + (5310 ± 197)/T where KH is in M atm(-1) and temperature is in K. This plus the salting out coefficient allow calculation of KH for any estuarine or sea water between 1 and 35 °C. High concentrations of dissolved organic carbon do not affect KH values in fresh or seawater...
May 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28517940/thermal-decomposition-of-potential-ester-biofuels-part-i-methyl-acetate-and-methyl-butanoate
#4
Jessica P Porterfield, David H Bross, Branko Ruscic, James H Thorpe, Thanh Lam Nguyen, Joshua H Baraban, John F Stanton, John W Daily, G Barney Ellison
Two methyl esters have been examined as models for the pyrolysis of biofuels. Dilute samples (0.06 - 0.13%) of methyl acetate (CH3COOCH3) and methyl butanoate (CH3CH2CH2COOCH3) were entrained in (He, Ar) carrier gas and decomposed in a set of flash-pyrolysis micro-reactors. The pyrolysis products resulting from the methyl esters were detected and identified by vacuum ultraviolet photoionization mass spectrometry. Complementary product identification was provided by matrix infrared absorption spectroscopy. Pyrolysis pressures in the pulsed micro-reactor were roughly 20 Torr and residence times through the reactors were approximately 25 - 150 µs...
May 18, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28515773/building-a-multipurpose-insertional-mutant-library-for-forward-and-reverse-genetics-in-chlamydomonas
#5
Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng, Kaiyao Huang
BACKGROUND: The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system, is not widely used in this organism. Screening of random insertional mutant libraries by polymerase chain reaction provides an alternate strategy to obtain null mutants of individual gene. But building, screening, and maintaining such a library was time-consuming and expensive...
2017: Plant Methods
https://www.readbyqxmd.com/read/28512003/towards-a-sustainable-biobased-industry-highlighting-the-impact-of-extremophiles
#6
REVIEW
Anna Krüger, Christian Schäfers, Carola Schröder, Garabed Antranikian
The transition of the oil-based economy towards a sustainable economy completely relying on biomass as renewable feedstock requires the concerted action of academia, industry, politics and civil society. An interdisciplinary approach of various fields such as microbiology, molecular biology, chemistry, genetics, chemical engineering and agriculture in addition to cross-sectional technologies such as economy, logistics and digitalization is necessary to meet the future global challenges. The genomic era has contributed significantly to the exploitation of naturés biodiversity also from extreme habitats...
May 13, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28511724/production-of-a-recombinant-swollenin-from-trichoderma-harzianum-in-escherichia-coli-and-its-potential-synergistic-role-in-biomass-degradation
#7
Clelton A Santos, Jaire A Ferreira-Filho, Anthonia O'Donovan, Vijai K Gupta, Maria G Tuohy, Anete P Souza
BACKGROUND: Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of cellulose structure by loosening macrofibrils. Consequently, SWOs can increase the accessibility and efficiency of the other enzymes involved in the saccharification of cellulosic substrates. Thus, SWOs are promising targets for improving the hydrolysis of plant biomass and for use as an additive to enhance the efficiency of an enzyme cocktail designed for the production of biofuels...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28508504/aiba-aibb-induces-an-intramolecular-decarboxylation-in-isovalerate-biosynthesis-by-myxococcus-xanthus
#8
Tobias Bock, Eva Luxenburger, Judith Hoffmann, Vlad Schütza, Christian Feiler, Rolf Müller, Wulf Blankenfeldt
Isovaleryl coenzyme A (IV-CoA) is an important precursor for iso-fatty acids and lipids. It acts in the development of myxobacteria, which can produce this compound from acetyl-CoA through alternative IV-CoA biosynthesis (aib). A central reaction of aib is catalyzed by AibA/AibB, which acts as a cofactor-free decarboxylase despite belonging to the family of CoA-transferases. We developed an efficient expression system for AibA/AibB that allowed the determination of high-resolution crystal structures in complex with different ligands...
May 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28507542/reactive-oxygen-species-mediated-cellular-stress-response-and-lipid-accumulation-in-oleaginous-microorganisms-the-state-of-the-art-and-future-perspectives
#9
REVIEW
Kun Shi, Zhen Gao, Tian-Qiong Shi, Ping Song, Lu-Jing Ren, He Huang, Xiao-Jun Ji
Microbial oils, which are mainly extracted from yeasts, molds, and algae, have been of considerable interest as food additives and biofuel resources due to their high lipid content. While these oleaginous microorganisms generally produce only small amounts of lipids under optimal growth conditions, their lipid accumulation machinery can be induced by environmental stresses, such as nutrient limitation and an inhospitable physical environmental. As common second messengers of many stress factors, reactive oxygen species (ROS) may act as a regulator of cellular responses to extracellular environmental signaling...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28505306/secretion-of-2-3-dihydroxyisovalerate-as-a-limiting-factor-for-isobutanol-production-in-saccharomyces-cerevisiae
#10
Wesley Cardoso Generoso, Martin Brinek, Heiko Dietz, Mislav Oreb, Eckhard Boles
Isobutanol is a superior biofuel compared to ethanol, and it is naturally produced by yeasts. Previously, Saccharomyces cerevisiae has been genetically engineered to improve isobutanol production. We found that yeast cells engineered for a cytosolic isobutanol biosynthesis secrete large amounts of the intermediate 2,3-dihydroxyisovalerate (DIV). This indicates that the enzyme dihydroxyacid dehydratase (Ilv3) is limiting the isobutanol pathway and/or yeast exhibit effective transport systems for the secretion of the intermediate, competing with isobutanol synthesis...
May 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28503197/the-significance-of-proline-and-glutamate-on-butanol-chaotropic-stress-in-bacillus-subtilis-168
#11
Gumpanat Mahipant, Atchara Paemanee, Sittiruk Roytrakul, Junichi Kato, Alisa S Vangnai
BACKGROUND: Butanol is an intensively used industrial solvent and an attractive alternative biofuel, but the bioproduction suffers from its high toxicity. Among the native butanol producers and heterologous butanol-producing hosts, Bacillus subtilis 168 exhibited relatively higher butanol tolerance. Nevertheless, organic solvent tolerance mechanisms in Bacilli and Gram-positive bacteria have relatively less information. Thus, this study aimed to elucidate butanol stress responses that may involve in unique tolerance of B...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28503195/strategies-for-improved-isopropanol-butanol-production-by-a-clostridium-strain-from-glucose-and-hemicellulose-through-consolidated-bioprocessing
#12
Fengxue Xin, Tianpeng Chen, Yujiang Jiang, Weiliang Dong, Wenming Zhang, Min Zhang, Hao Wu, Jiangfeng Ma, Min Jiang
BACKGROUND: High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28501381/salinity-induced-oxidative-stress-alters-the-physiological-responses-and-improves-the-biofuel-potential-of-green-microalgae-acutodesmus-dimorphus
#13
Kaumeel Chokshi, Imran Pancha, Arup Ghosh, Sandhya Mishra
The main aim of the present study was to analyze salinity stress induced physiological and biochemical changes in a freshwater microalgae Acutodesmus dimorphus. During single-stage cultivation, the accumulations of lipids and carbohydrates increased with an increase in an initial salinity of the culture medium. The carbohydrate and lipid accumulations of 53.30±2.76% and 33.40±2.29%, respectively, were observed in 200mM NaCl added culture. During two-stage cultivation, salinity stress of 200mM was favorable for the growth up to 2days, as suggested by higher biomass, lower levels of oxidative stress biomarkers and no significant changes in the biochemical composition of the cells...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28498495/oleaginous-yeasts-promising-platforms-for-the-production-of-oleochemicals-and-biofuels
#14
José L Adrio
Oleaginous yeasts have a unique physiology that makes them the best suited hosts for the production of lipids, oleochemicals and diesel-like fuels. Their high lipogenesis, capability of growing on many different carbon sources (including lignocellulosic sugars), easy large-scale cultivation, and an increasing number of genetic tools are some of the advantages that have encouraged their use to develop sustainable processes. This mini-review summarizes the metabolic engineering strategies developed in oleaginous yeasts within the last two years to improve process metrics (titer, yield and productivity) for the production of lipids, free fatty acids, fatty acid-based chemicals (eg...
May 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28494761/production-of-fuels-and-chemicals-from-xylose-by-engineered-saccharomyces-cerevisiae-a-review-and-perspective
#15
REVIEW
Suryang Kwak, Yong-Su Jin
Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals. A robust ethanol producing yeast Saccharomyces cerevisiae has been engineered with heterologous xylose assimilation pathways. A two-step oxidoreductase pathway consisting of NAD(P)H-linked xylose reductase and NAD(+)-linked xylitol dehydrogenase, and one-step isomerase pathway using xylose isomerase have been employed to enable xylose assimilation in engineered S...
May 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#16
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28487674/uncovering-potential-applications-of-cyanobacteria-and-algal-metabolites-in-biology-agriculture-and-medicine-current-status-and-future-prospects
#17
REVIEW
Rachana Singh, Parul Parihar, Madhulika Singh, Andrzej Bajguz, Jitendra Kumar, Samiksha Singh, Vijay P Singh, Sheo M Prasad
Cyanobacteria and algae having complex photosynthetic systems can channelize absorbed solar energy into other forms of energy for production of food and metabolites. In addition, they are promising biocatalysts and can be used in the field of "white biotechnology" for enhancing the sustainable production of food, metabolites, and green energy sources such as biodiesel. In this review, an endeavor has been made to uncover the significance of various metabolites like phenolics, phytoene/terpenoids, phytols, sterols, free fatty acids, photoprotective compounds (MAAs, scytonemin, carotenoids, polysaccharides, halogenated compounds, etc...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28486485/hydrogen-peroxide-inhibition-of-bicupin-oxalate-oxidase
#18
John M Goodwin, Hassan Rana, Joan Ndungu, Gaurab Chakrabarti, Ellen W Moomaw
Oxalate oxidase is a manganese containing enzyme that catalyzes the oxidation of oxalate to carbon dioxide in a reaction that is coupled with the reduction of oxygen to hydrogen peroxide. Oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx) is the first fungal and bicupin enzyme identified that catalyzes this reaction. Potential applications of oxalate oxidase for use in pancreatic cancer treatment, to prevent scaling in paper pulping, and in biofuel cells have highlighted the need to understand the extent of the hydrogen peroxide inhibition of the CsOxOx catalyzed oxidation of oxalate...
2017: PloS One
https://www.readbyqxmd.com/read/28484517/h2-production-pathways-in-nutrient-replete-mixotrophic-chlamydomonas-cultures-under-low-light-response-to-the-commentary-article-on-the-pathways-feeding-the-h2-production-process-in-nutrient-replete-hypoxic-conditions-by-alberto-scoma-and-szilvia-z-t%C3%A3-th
#19
EDITORIAL
David González-Ballester, Jose Luis Jurado-Oller, Aurora Galván, Emilio Fernández, Alexandra Dubini
BACKGROUND: A recent Commentary article entitled "On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions" by Dr. Scoma and Dr. Tóth, Biotechnology for Biofuels (2017), opened a very interesting debate about the H2 production photosynthetic-linked pathways occurring in Chlamydomonas cultures grown in acetate-containing media and incubated under hypoxia/anoxia conditions. This Commentary article mainly focused on the results of our previous article "Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures," by Jurado-Oller et al...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484516/on-the-pathways-feeding-the-h2-production-process-in-nutrient-replete-hypoxic-conditions-commentary-on-the-article-low-oxygen-levels-contribute-to-improve-photohydrogen-production-in-mixotrophic-non-stressed-chlamydomonas-cultures-by-jurado-oller-et-al-biotechnology
#20
EDITORIAL
Alberto Scoma, Szilvia Z Tóth
BACKGROUND: Under low O2 concentration (hypoxia) and low light, Chlamydomonas cells can produce H2 gas in nutrient-replete conditions. This process is hindered by the presence of O2, which inactivates the [FeFe]-hydrogenase enzyme responsible for H2 gas production shifting algal cultures back to normal growth. The main pathways accounting for H2 production in hypoxia are not entirely understood, as much as culture conditions setting the optimal redox state in the chloroplast supporting long-lasting H2 production...
2017: Biotechnology for Biofuels
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