Rafael Leandro Fernandes Melo, Tiago Melo Freire, Roberta Bussons Rodrigues Valério, Francisco Simão Neto, Viviane de Castro Bizerra, Bruno Caio Chaves Fernandes, Paulo Gonçalves de Sousa Junior, Aluísio Marques da Fonseca, João Maria Soares, Pierre Basílio Almeida Fechine, José Cleiton Sousa Dos Santos
Magnetic nanoparticles were functionalized with polyethylenimine (PEI) and activated with epoxy. This support was used to immobilize Lipase (Eversa® Transform 2.0) (EVS), optimization using the Taguchi method. XRF, SEM, TEM, XRD, FTIR, TGA, and VSM performed the characterizations. The optimal conditions were immobilization yield (I.Y.) of 95.04 ± 0.79 %, time of 15 h, ionic load of 95 mM, protein load of 5 mg/g, and temperature of 25 °C. The maximum loading capacity was 25 mg/g, and its stability in 60 days of storage showed a negligible loss of only 9...
March 8, 2024: International Journal of Biological Macromolecules