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https://www.readbyqxmd.com/read/29778819/detection-of-tem-and-ctx-m-genes-from-ciprofloxacin-resistant-proteus-mirabilis-and-escherichia-coli-isolated-on-urinary-tract-infections-utis
#1
Govindan Rajivgandhi, Muthuchamy Maruthupandy, Natesan Manoharan
The multidrug resistant Gram negative bacteria (MDRGNB) is an emerging burden and now represents a daily challenge for the management of antimicrobial therapy in healthcare settings. The present study was aimed to detect the prevalence of TEM and CTX-M type genes from GNB on urinary tract infection (UTIs). The ciprofloxacin resistant uropathogens were detected by HEXA UTI 5 disc diffusion method. The phenotypic detection of uropathogens producing extended spectrum beta lactamases (ESBLs) was confirmed by double disc combination (DDCT) and phenotype confirmation methods (PCT)...
May 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29778048/pathogens-constancy-harbinger-of-nosocomial-infection-cum-identification-of-resistant-genes-and-drug-designing
#2
Sana Farooq Ahmad, Ibrar Khan, Abdul Wadood, Sadiq Azam, Noor Rehman, Muhammad Waqas, Kashif Bashir, Asaf Ali Khan
Hospitals are the most prominent places for the growth and spread of bacteria which are resistant to the available antibiotics. This antibiotic resistance is due to the over and misuse of antibiotic dosages of a high-density of patient population which are in frequent interaction with inanimate items of the hospitals and the consequent risk of cross infection. Out of 1010 samples of the current study, 510 (50.49%) were culture positive of which 329 (64.5%) were Gram-positive while 181 (35.49%) Gram negative...
April 22, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29777948/escherichia-coli-hyperepidemic-clone-st410-a-harboring-bla-ctx-m-15-isolated-from-fresh-vegetables-in-a-municipal-market-in-quito-ecuador
#3
David Ortega-Paredes, Pedro Barba, Santiago Mena-López, Nathaly Espinel, Jeannete Zurita
Dissemination of Extended spectrum β-lactamases (ESBL) Enterobacteriaceae is a major medical threat. Vegetables and fruits, which are usually consumed raw, are a very suitable pathway for the spread of these bacteria from farm-to-fork. However, limited information exists regarding resistant bacteria and epidemic clones that are disseminated in vegetables and tap water in South America. We processed a total of 90 samples in triplicate of nine typically consumed raw vegetables from a central municipal market, and tap water samples were processed from twenty-one locations in Quito, Ecuador...
April 30, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29761673/neonatal-risk-factors-for-colonization-with-extended-spectrum-beta-lactamase-producing-bacteria-in-the-neonatal-intensive-care-unit
#4
Viktoria Leikin-Zach, Eilon Shany, Maayan Yitshak-Sade, Ron Eshel, Tali Shafat, Avraham Borer, Rimma Melamed
BACKGROUND: Extended-spectrum beta-lactamase (ESBL) production is the most common antimicrobial resistance mechanism in the neonatal intensive care unit (NICU), with colonization and blood stream infections being a major threat to this population. Since 2013, all NICU admissions at our facility were screened twice weekly for ESBL colonization. OBJECTIVES: To determine independent risk factors for colonization of infants with ESBL-producing bacteria in the NICU. METHODS: A retrospective case study of ESBL-colonized infants vs...
May 2018: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/29760961/ctx-m-15-is-established-in-most-multidrug-resistant-uropathogenic-enterobacteriaceae-and-pseudomonaceae-from-hospitals-in-nigeria
#5
David Olusoga Ogbolu, O A Terry Alli, Mark Alexander Webber, Adeolu Sunday Oluremi, Omoboriowo Moses Oloyede
β-Lactam antibiotics are widely used to treat urinary tract infections in Nigeria. This study aimed to determine the presence and characteristics of extended spectrum β-lactamases in commonly isolated uropathogenic Gram-negative bacteria (GNB) in Nigeria. Fifty non-duplicate GNB isolates consisting of Escherichia coli , 19; Klebsiella pneumoniae , 21; and Pseudomonas aeruginosa , 10 were obtained from three tertiary hospitals in Nigeria. The antibiotic susceptibility testing of all isolates to a panel of antibiotics including minimum inhibitory concentrations (MICs) and extended spectrum β-lactamases was determined...
April 2018: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29751417/occurrence-and-characteristics-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-bacteria-from-hospital-effluents-in-singapore
#6
Laurence Haller, Hongjie Chen, Charmaine Ng, Thai Hoang Le, Tse Hsien Koh, Timothy Barkham, Mark Sobsey, Karina Yew-Hoong Gin
One of the most important resistance mechanisms in Gram-negative bacteria today is the production of enzymes causing resistance to cephalosporin and carbapenem antibiotics. The spread of extended-spectrum β-lactamases (ESBL)- and carbapenemase- producing Gram-negative bacteria is an emerging global public health problem. The aim of the present study was to (i) assess the prevalence of carbapenem-resistant bacteria (CRB) and ESBL-producing strains in sewage effluents from two major hospitals in Singapore, (ii) characterize the isolated strains and (iii) identify some of the ESBL and carbapenemase genes responsible for the resistance...
February 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29751120/evaluation-of-a-risk-guided-strategy-for-empiric-carbapenem-use-in-febrile-neutropenia
#7
Ai Leng Khoo, Ying Jiao Zhao, Monica Teng, Ding Ying, Jing Jin, Yen Lin Chee, Li Mei Poon, Siew Eng Lim, Liang Piu Koh, Wee Joo Chng, Boon Peng Lim, Li Yang Hsu, Louis Yi Ann Chai
Febrile neutropenia (FN) is associated with substantial morbidity and necessitate empiric broad spectrum antibiotic treatment. We evaluated a risk-guided FN management strategy using empiric piperacillin-tazobactam and carbapenem in a prospective cohort study. The analysis involved 723 FN episodes in hospitalised adult patients, including those with severe sepsis or prior infection/colonisation with extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Propensity score matching analysis was used to adjust for baseline differences between treatment groups and produced 267 matched pairs...
May 8, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29743768/epidemiological-analysis-of-extended-spectrum-beta-lactamase-producing-bacterial-infections-in-adult-live-donor-liver-transplant-patients
#8
Ajeet Singh, Deepak Govil, Usha Krishan Baveja, Anand Gupta, Neha Tandon, Shrikanth Srinivasan, Sachin Gupta, Sweta J Patel, Sanjiv Saigal, Arvinder Singh Soin
Introduction: Bacterial infections are a leading cause of morbidity and mortality in patients receiving solid-organ transplants. Extended-spectrum beta-lactamases (ESBL) pathogens are the most important pathogenic bacteria infecting these patients. Aim: This study aims to evaluate for the incidence and characteristics of ESBL-positive organism, to look for the clinical outcomes in ESBL-positive infected cases, and to evaluate and draft the antibiotic policy in posttransplant patients during the first 28 days posttransplant...
April 2018: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/29738654/-multidrug-resistance-e-eskape-strains-isolated-from-blood-cultures-in-patients-with-cancer
#9
Consuelo Velázquez-Acosta, Patricia Cornejo-Juárez, Patricia Volkow-Fernández
OBJECTIVE: To describe the trend of multidrug resistant (MDR) strains isolated from blood in patients with cancer from 2005 to 2015. MATERIAL AND METHODS: 33 127 blood cultures were processed by retrospective analysis. Identification and antimicrobial sensitivity were performed through automated methods: WaLK away (Siemens Labora- tory Diagnostics) and BD Phoenix (Becton, Dickinson and Company). Resistant strains were determined according to the minimum inhibitory concentration, following the parameters of the Clinical and Laboratory Standards Institute (CLSI)...
March 2018: Salud Pública de México
https://www.readbyqxmd.com/read/29735823/antimicrobial-susceptibility-profiles-of-gram-negative-bacteria-causing-infections-collected-across-india-during-2014-2016-study-for-monitoring-antimicrobial-resistance-trend-report
#10
Balaji Veeraraghavan, Mark Ranjan Jesudason, John Antony Jude Prakasah, Shalini Anandan, Rani Diana Sahni, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Rajesh Joseph Selvakumar, T N Dhole, Camilla Rodrigues, Indranil Roy, Sangeetha Joshi, Bhaskar Narayan Chaudhuri, D S Chitnis
Background: The emergence of antibiotic resistance among bacterial pathogens in the hospital and community has increased the concern to the health-care providers due to the limited treatment options. Surveillance of antimicrobial resistance (AMR) in frequently isolated bacterial pathogens causing severe infections is of great importance. The data generated will be useful for the clinicians to decide empiric therapy on the local epidemiological resistance profile of the antimicrobial agents...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29734648/bioactivity-guided-isolation-of-antimicrobial-and-antioxidant-metabolites-from-the-mushroom-tapinella-atrotomentosa
#11
Zoltán Béni, Miklós Dékány, Bernadett Kovács, Boglárka Csupor-Löffler, Zoltán Péter Zomborszki, Erika Kerekes, András Szekeres, Edit Urbán, Judit Hohmann, Attila Ványolós
Bioassay-guided fractionation of the chloroform extract of Tapinella atrotomentosa led to the isolation of four secondary metabolites 1 ⁻ 4 . Two of the compounds are lactones—osmundalactone ( 1 ) and 5-hydroxy-hex-2-en-4-olide ( 2 )—while 3 and 4 were identified as terphenyl quinones, spiromentins C and B, respectively. The structures of the compounds were established on the basis of NMR and MS spectroscopic analysis. The isolated fungal metabolites were evaluated for their antibacterial activities against several Gram-positive and negative bacteria...
May 4, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29731647/ambulance-vehicles-as-a-source-of-multidrug-resistant-infections-a-multicenter-study-in-assiut-city-egypt
#12
Mohamed A El-Mokhtar, Helal F Hetta
Background: Ambulances may represent a potential source of infection to patients, patients' relatives, and paramedical staffs. In this study, we analyzed the extent of bacterial contamination in ambulance vehicles and measured the degree of antimicrobial resistance among isolated pathogens. Materials and methods: Twenty-five vehicles were included and 16 sampling points were swabbed in each vehicle. Then the swabs were immediately transferred to the laboratory to identify bacterial contaminants utilizing standard microbiological procedures and API® systems...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29731643/ugly-bugs-in-healthy-guts-carriage-of-multidrug-resistant-and-esbl-producing-commensal-enterobacteriaceae-in-the-intestine-of-healthy-nepalese-adults
#13
Anjila Maharjan, Anjeela Bhetwal, Shreena Shakya, Deepa Satyal, Shashikala Shah, Govardhan Joshi, Puspa Raj Khanal, Narayan Prasad Parajuli
Background: Fecal carriage of multidrug-resistant and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is one of the important risk factors for infection with antibiotic-resistant bacteria. In this report, we examined the prevalence of multidrug-resistant and ESBL-producing common enterobacterial strains colonizing the intestinal tract of apparently healthy adults in Kathmandu, Nepal. Methods: During a 6-month period (February-July 2016), a total of 510 stool specimens were obtained from apparently healthy students of Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29718820/characteristics-of-surgical-site-infection-following-colorectal-surgery-in-a-tertiary-center-extended-spectrum-%C3%AE-lactamase-producing-bacteria-culprits-in-disease
#14
Eliana Kalakouti, Constantinos Simillis, Gianluca Pellino, Nabeela Mughal, Oliver Warren, Sarah Mills, Emile Tan, Christos Kontovounisios, Paris P Tekkis
INTRODUCTION: Surgical site infection (SSI) is a well-known complication of colorectal surgery associated with increased morbidity and hospital stay. Antimicrobial prophylaxis can reduce the risk of SSI by as much as 75%. Extended-spectrum β-lactamase (ESBL)-producing pathogens make the successful use of such prophylaxis a challenge and are a real threat to patient care following colorectal surgery. OBJECTIVE: The aim of this study is to report the common characteristics of SSIs after colorectal surgery and to highlight the prevalence, risk factors, and clinical relevance of ESBL infections among these patients in a tertiary center...
December 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29710862/antimicrobial-activity-of-new-materials-based-on-lavender-and-basil-essential-oils-and-hydroxyapatite
#15
Daniela Predoi, Simona Liliana Iconaru, Nicolas Buton, Monica Luminita Badea, Luminita Marutescu
This study presents, for the first-time, the results of a study on the hydrodynamic diameter of essential oils (EOs) of basil and lavender in water, and solutions of EOs of basil (B) and lavender (L) and hydroxyapatite (HAp). The possible influence of basil and lavender EOs on the size of hydroxyapatite nanoparticles was analyzed by Scanning Electron Microscopy (SEM). We also investigated the in vitro antimicrobial activity of plant EOs and plant EOs hydroxyapatite respectively, against Gram-positive bacteria (methicillin-resistant Staphylococcus aureus 1144 (MRSA 1144) and S...
April 30, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29709404/microbiology-of-french-military-casualties-repatriated-from-overseas-for-an-open-traumatic-injury
#16
S Larréché, A Bousquet, C Soler, C Mac Nab, D de Briel, D Delaune, C Bigaillon, P Pasquier, C Dubost, T Demoures, B Malgras, S Ausset, S de Rudnicki, T Leclerc, B de Loynes, S Bonnet, N Mocellin, C Ficko, R Haus, O Hersan, S Rigal, A Mérens
BACKGROUND: This study aimed to describe the microbiological epidemiology of repatriated French soldiers with an open traumatic injury, and to measure the proportion of multidrug-resistant bacteria (MDRB). METHODS: Retrospective study including all French soldiers repatriated in 2011 and 2012 in Parisian military hospitals for open traumatic injury. Results of clinical samples and MDRB screening were collected. The antibiotic susceptibility was assessed using the agar disk diffusion method...
April 27, 2018: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29705860/bloodstream-infections-in-haematological-cancer-patients-colonized-by-multidrug-resistant-bacteria
#17
C Cattaneo, R Di Blasi, C Skert, A Candoni, B Martino, N Di Renzo, M Delia, S Ballanti, F Marchesi, V Mancini, E Orciuolo, S Cesaro, L Prezioso, R Fanci, G Nadali, A Chierichini, L Facchini, M Picardi, M Malagola, V Orlando, E M Trecarichi, M Tumbarello, F Aversa, G Rossi, L Pagano
Infections by multidrug-resistant (MDR) bacteria are a worrisome phenomenon in hematological patients. Data on the incidence of MDR colonization and related bloodstream infections (BSIs) in haematological patients are scarce. A multicentric prospective observational study was planned in 18 haematological institutions during a 6-month period. All patients showing MDR rectal colonization as well as occurrence of BSI at admission were recorded. One-hundred forty-four patients with MDR colonization were observed (6...
April 28, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29692574/phenotypic-and-genotypic-characterization-of-carbapenem-resistance-mechanisms-in-klebsiella-pneumoniae-from-blood-culture-specimens-a-study-from-north-india
#18
Varsha Gupta, Ritu Garg, Karthikeyan Kumaraswamy, Priya Datta, Gursimran Kaur Mohi, Jagdish Chander
BACKGROUND: Emergence of carbapenem resistance among Enterobacteriaceae in different geographical regions is of great concern as these bacteria are easily transmissible among patients. Carbapenem-resistance in Enterobacteriaceae is due to production of carbapenemases of various classes and hyper production of the ESBLs (Extended spectrum beta lactamases) and Amp C beta lactamases with reduced cell wall permeability mechanisms. Phenotypic detection and differentiation is important for proper infection control and appropriate patient management...
April 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/29690684/-the-composition-and-antimicrobial-resistance-of-isolates-from-lower-respiratory-tract-and-blood-in-hospitalized-patients-in-respiratory-ward-a-multicenter-national-study-in-china
#19
X Tang, C Zhuo, Y C Xu, N S Zhong
Objective: To investigate the species and antimicrobial resistance of bacterial pathogens isolated from hospitalized patients in respiratory ward in China. Methods: This was a multicenter retrospective study based on a national epidemiological network called China Antimicrobial Resistance Surveillance System (CARSS). The non-repetitive strains isolated from lower respiratory tract and blood samples in 91 hospitals from seven geographic regions of CARSS were reviewed. The distribution of specimen type, hospital level (secondary and tertiary hospital), patient age group [geriatric (>65 years old), adult (15 to 65 years old), pediatric (28 days to 14 years old ) and newborn group (≤28 days)] and ward type (respiratory intensive care unit and general respiratory ward) were analyzed for MRSA, PRSP, CREC, CRKP, CRPA, CRAB, ESBL-EC and ESBL-KP...
April 12, 2018: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/29685852/assessment-of-antimicrobiral-prophylaxis-in-transperineal-prostate-biopsy-a-single-center-retrospective-study-of-485-cases
#20
Kyoko Baba, Yoshitaka Sekine, Yoshiyuki Miyazawa, Takahiro Syuto, Masashi Nomura, Hidekazu Koike, Hiroshi Matsui, Yasuhiro Shibata, Kazuto Ito, Kazuhiro Suzuki
To verify the validity of our antimicrobial prophylaxis regimen for transperineal prostate biopsies, we investigated the rate of infectious complications in this procedure. We retrospectively investigated the infectious complications in 485 patients who underwent a transperineal prostate biopsy between 2014 and 2016 at our hospital. In the clinic, we use cefazolin (CEZ) for antimicrobial prophylaxis. Infectious complications were assessed up to postoperative day (POD) 30. Patients with infectious complications were further investigated to determine the site of infection, outbreak day, and type of pathogenic bacteria...
April 20, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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