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Mycobacterium tuberculosis Beijing

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https://www.readbyqxmd.com/read/28734764/comparative-genomic-analysis-of-mycobacterium-tuberculosis-beijing-like-strains-revealed-specific-genetic-variations-associated-with-virulence-and-drug-resistance
#1
Juan Germán Rodríguez-Castillo, Camilo Pino, Luis Fernando Niño, Juan Carlos Rozo, Claudia Llerena-Polo, Carlos A Parra-López, Andreas Tauch, Martha Isabel Murcia-Aranguren
Isolates of the Mycobacterium tuberculosis lineage 2/East-Asian are considered one of the most successful strains due to their increased pathogenicity, hyper-virulence associated with drug resistance, and high transmission. Recent studies in Colombia have shown that the Beijing-like genotype is associated with multidrug-resistance and high prevalence in the southwest of the country, but the genetic basis of its success in dissemination is unknown. In contribution to this matter, we obtained the whole sequences of six genomes of clinical isolates assigned to the Beijing-like genotype...
July 19, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28729708/new-mycobacterium-tuberculosis-beijing-clonal-complexes-in-china-revealed-by-phylogenetic-and-bayesian-population-structure-analyses-of-24-loci-miru-vntrs
#2
Chao Zheng, Yann Reynaud, Changsong Zhao, Thierry Zozio, Song Li, Dongxia Luo, Qun Sun, Nalin Rastogi
Beijing lineage of Mycobacterium tuberculosis constitutes the most predominant lineage in East Asia. Beijing epidemiology, evolutionary history, genetics are studied in details for years revealing probable origin from China followed by worldwide expansion, partially linked to higher mutation rate, hypervirulence, drug-resistance, and association with cases of mixed infections. Considering huge amount of data available for 24-loci Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats, we performed detailed phylogenetic and Bayesian population structure analyses of Beijing lineage strains in mainland China and Taiwan using available 24-loci MIRU-VNTR data extracted from publications or the SITVIT2 database (n = 1490)...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710410/combining-molecular-typing-and-spatial-pattern-analysis-to-identify-areas-of-high-tuberculosis-transmission-in-a-moderate-incidence-county-in-taiwan
#3
Yih-Yuan Chen, Jia-Ru Chang, Chih-Da Wu, Yen-Po Yeh, Shiu-Ju Yang, Chih-Hao Hsu, Ming-Ching Lin, Ching-Fang Tsai, Ming-Shian Lin, Ih-Jen Su, Horng-Yunn Dou
In total, 303 randomly selected clinical Mycobacterium tuberculosis (MTB) isolates from 303 patients (collected January to December 2012) in central Taiwan were examined. The major lineages found were Beijing (N = 114, 37.62%), Haarlem (N = 76, 25.08%) and East African-Indian (EAI) (N = 42, 13.86%). Notably, younger persons (≤30 years old) were 6.58 times more likely to be infected with a Beijing genotype compared to older persons (>70 years) (p < 0.05). Combining molecular typing methods and geographical information system (GIS) analysis, we uncovered a twofold higher incidence of Beijing strains in a hotspot area (33%) compared to non-hotspot areas (17%)...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28684565/complete-genome-sequences-of-three-representative-mycobacterium-tuberculosis-beijing-family-strains-belonging-to-distinct-genotype-clusters-in-hanoi-vietnam-during-2007-to-2009
#4
Takayuki Wada, Minako Hijikata, Shinji Maeda, Nguyen Thi Le Hang, Pham Huu Thuong, Nguyen Phuong Hoang, Nguyen Van Hung, Naoto Keicho
We present here three complete genome sequences of Mycobacterium tuberculosis Beijing family strains isolated in Hanoi, Vietnam. These three strains were selected from major genotypic clusters (15-MIRU-VNTR) identified in a previous population-based study. We emphasize their importance and potential as reference strains in this Asian region.
July 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28683213/proteomics-of-culture-filtrate-of-prevalent-mycobacterium-tuberculosis-strains-2d-page-map-and-maldi-tof-ms-analysis
#5
Gavish Kumar, Hari Shankar, Divakar Sharma, Prashant Sharma, Deepa Bisht, Vishwa M Katoch, Beenu Joshi
Although diverse efforts have been done to identify biomarkers for control of tuberculosis using laboratory strain Mycobacterium tuberculosis H37Rv, the disease still poses a threat to mankind. There are many emerging M. tuberculosis strains, and proteomic profiling of these strains might be important to find out potential targets for diagnosis and/or prevention of tuberculosis. We evaluated the comparative proteomic profiling of culture filtrate (CF) proteins from prevalent M. tuberculosis strains (Central Asian or Delhi type; CAS1_Del, East African-Indian; EAI-3 and Beijing family) by 2D polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28669673/investigation-of-a-pre-xdr-beijing-mycobacterium-tuberculosis-transmission-to-a-healthcare-worker-in-france-2016
#6
Anaïs Appelgren, David Morquin, Sébastien Dufour, Vincent Le Moing, Jacques Reynes, Anne Lotthé, Sylvie Parer, Catherine Corbeau, Alexandra Aubry, Wladimir Sougakoff, Jérôme Solassol, Lucas Bonzon, Yann Dumont, Sylvain Godreuil
We report a case of occupational contamination of a healthcare worker (HCW) by a pre-extensively drug-resistant (pre-XDR) Beijing strain of Mycobacterium tuberculosis in the University Hospital of Montpellier, France. The index case was identified using the genetic fingerprinting of isolates. This report underscores the risk of healthcare-associated contamination by pre-XDR TB in low-incidence countries, the importance of molecular tools for tuberculosis care, and calls for increased vigilance in the management of MDR/XDR TB patients...
June 29, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28659320/development-of-a-one-step-multiplex-pcr-assay-for-differential-detection-of-major-mycobacterium-species
#7
Hansong Chae, Seung Jung Han, Su Young Kim, Chang-Seok Ki, Hee Jae Huh, Dongeun Yong, Won-Jung Koh, Sung Jae Shin
The prevalence of tuberculosis continues to be high, and nontuberculous mycobacteria (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of this assay were to: 1) discriminate between the Mycobacterium tuberculosis complex (MTBC) and NTM using rv0577 or RD750; 2) differentiate M...
June 28, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28610791/the-role-of-the-time-kill-kinetics-assay-as-part-of-a-preclinical-modeling-framework-for-assessing-the-activity-of-anti-tuberculosis-drugs
#8
Hannelore I Bax, Irma A J M Bakker-Woudenberg, Corné P de Vogel, Aart van der Meijden, Annelies Verbon, Jurriaan E M de Steenwinkel
Novel treatment strategies for tuberculosis are urgently needed. Many different preclinical models assessing anti-tuberculosis drug activity are available, but it is yet unclear which combination of models is most predictive of clinical treatment efficacy. The aim of this study was to determine the role of our in vitro time kill-kinetics assay as an asset to a predictive preclinical modeling framework assessing anti-tuberculosis drug activity. The concentration- and time-dependent mycobacterial killing capacities of six anti-tuberculosis drugs were determined during exposure as single drugs or in dual, triple and quadruple combinations towards a Mycobacterium tuberculosis Beijing genotype strain and drug resistance was assessed...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28610789/comparative-study-of-genotypes-of-mycobacterium-tuberculosis-from-a-northern-indian-setting-with-strains-reported-from-other-parts-of-india-and-neighboring-countries
#9
Pragya Sharma, Kiran Katoch, Shilpi Chandra, Devendra Singh Chauhan, Vishnu Dutt Sharma, David Couvin, Nalin Rastogi, Vishwa Mohan Katoch
This study was carried out to characterize Mycobacterium tuberculosis population in Ghatampur, Kanpur, North India, by spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTRs) typing. A total of 335 isolates were genotyped by spoligotyping and Central Asian (CAS) sub-lineage was the most prevalent, comprising 59.1% of all isolates. Other lineages were: East-African Indian (EAI) (19.10%), T (5.07%), Beijing (3.28%), Manu (2.98%), X (2.68%), S (0.89%), H3 (0...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28596961/first-insight-into-the-molecular-epidemiology-of-mycobacterium-tuberculosis-isolates-from-the-minority-enclaves-of-southwestern-china
#10
Lianyong Chen, Yu Pang, Li Ma, Huijuan Yang, Haohao Ru, Xing Yang, Shuangqun Yan, Mao Jia, Lin Xu
Yunnan is a province located in southwestern China. The aim of this study was to investigate the genetic diversity of Mycobacterium tuberculosis (MTB) strains circulating in Yunnan Province. We used spoligotyping and a 12-locus high-resolution VNTR set to identify a total of 271 MTB isolates collected from six prefectures' Yunnan. All the 271 patients were classified as 11 different ethnic groups, including 133 Han patients (49.1%) and 138 minority patients (50.9%). Spoligotyping analyses revealed that the largest two spoligotypes were SIT1 (Beijing family, n = 136) and SIT53 (T family, n = 35)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28588286/mycobacterium-tuberculosis-beijing-genotype-family-strain-isolated-from-naturally-infected-plateau-zokor-myospalax-baileyi-in-china
#11
Lin Sun, Xiang Chen, Wenhai Zhang, Gencheng Huang, Yuanyuan Zhang, Zhengzhong Xu, Baofa Yin, Wanhong Wei, Xinan Jiao, Kanglin Wan
No abstract text is available yet for this article.
June 7, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28550826/-multidrug-resistant-tuberculosis-by-strains-of-beijing-family-in-patients-from-lisbon-portugal-preliminary-report
#12
Fernando Maltez, Teresa Martins, Diana Póvoas, João Cabo, Helena Peres, Francisco Antunes, João Perdigão, Isabel Portugal
INTRODUCTION: Beijing family strains of Mycobacterium tuberculosis are associated with multidrug-resistance. Although strains of the Lisboa family are the most common among multidrug-resistant and extensively drug-resistant patients in the region, several studies have reported the presence of the Beijing family. However, the features of patients from whom they were isolated, are not yet known. MATERIAL AND METHODS: Retrospective study involving 104 multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis, from the same number of patients, isolated and genotyped between 1993 and 2015 in Lisbon...
March 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28545488/genetic-diversity-of-mycobacterium-tuberculosis-isolates-from-tochigi-prefecture-a-local-region-of-japan
#13
Fuminori Mizukoshi, Tohru Miyoshi-Akiyama, Hiroki Iwai, Takako Suzuki, Reiko Kiritani, Teruo Kirikae, Keiji Funatogawa
BACKGROUND: Foreign-born patients with tuberculosis (TB) may introduce globally disseminated isolates of Mycobacterium tuberculosis into large cities in Japan. The risk of dissemination of these isolates into local regions, however, has not been determined. This study analyzed the molecular epidemiology of M. tuberculosis isolates obtained from TB patients living in a local region of Japan. METHODS: Whole genome sequences of 169 M. tuberculosis isolates, obtained from 148 Japanese-born and 21 foreign-born patients living in Tochigi, Japan, were analyzed using the Comprehensive analysis server for the Mycobacterium t u b erculosis complex (CASTB)...
May 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28527287/-identification-of-the-mycobacterium-tuberculosis-beijing-lineage-in-ecuador
#14
Patricia Jiménez, Karina Calvopiña, Diana Herrera, Carlos Rojas, Laura Pérez-Lago, Marcelo Grijalva, Remedios Guna, Darío García-de Viedma
INTRODUCTION: Mycobacterium tuberculosis Beijing lineage isolates are considered to be especially virulent, transmissible and prone to acquire resistances. Beijing strains have been reported worldwide, but studies in Latin America are still scarce. The only multinational study performed in the region indicated a heterogeneous distribution for this lineage, which was absent in Chile, Colombia and Ecuador, although further studies found the lineage in Chile and Colombia. OBJECTIVE: To search for the presence of the Beijing lineage in Ecuador, the only country in the region where it remains unreported...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28522873/recombinant-bcg-expressing-ltak63-adjuvant-induces-superior-protection-against-mycobacterium-tuberculosis
#15
Ivan P Nascimento, Dunia Rodriguez, Carina C Santos, Eduardo P Amaral, Henrique K Rofatto, Ana P Junqueira-Kipnis, Eduardo D C Gonçalves, Maria R D'Império-Lima, Mario H Hirata, Celio L Silva, Nathalie Winter, Brigitte Gicquel, Kingston H G Mills, Mariagrazia Pizza, Rino Rappuoli, Luciana C C Leite
In order to develop an improved BCG vaccine against tuberculosis we have taken advantage of the adjuvant properties of a non-toxic derivative of Escherichia coli heat labile enterotoxin (LT), LTAK63. We have constructed rBCG strains expressing LTAK63 at different expression levels. Mice immunized with BCG expressing low levels of LTAK63 (rBCG-LTAK63lo) showed higher Th1 cytokines and IL-17 in the lungs, and when challenged intratracheally with Mycobacterium tuberculosis displayed a 2.0-3.0 log reduction in CFU as compared to wild type BCG...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28506215/comparison-of-antibody-responses-against-mycobacterium-tuberculosis-antigen-rv0679c-in-tuberculosis-patients-from-the-endemic-and-non-endemic-regions-of-the-beijing-genotype-a-case-control-study
#16
Jingge Zhao, Takashi Matsuba, Xiaoyan Zhang, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Elizabeth Freda Telan, Yasuhiko Suzuki, Toshio Hattori
BACKGROUND: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses...
May 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28482417/-molecular-epidemiologic-study-on-mycobacterium-tuberculosis-from-drug-resistance-monitoring-sites-of-guangdong-province-2015
#17
X C Huang, H X Guo, Z H Wu, C X Guo, W J Wei, H C Li, Q Sun, C C Zhang, Z Y Li, T Chen, Q Zhong, L Zhou
Objective: To understand the characteristics of Mycobacterium tuberculosis (MTB) in epidemiology and distribution from Guangdong Province, and to explore the risk factors associated with drug resistance. Methods: A total of 225 clinical strains of MTB collected from 5 drug resistance monitoring sites of Guangdong Province in 2015 were tested by Regions of Difference 105 (RD105) deletion test and 15 loci mycobacterial interspersed repetitive units (MIRU) were used for genotyping. Gene clustering was analyzed using BioNumerics7...
May 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28482027/cmr-is-a-redox-responsive-regulator-of-dosr-that-contributes-to-m-tuberculosis-virulence
#18
Laura J Smith, Aleksandra Bochkareva, Matthew D Rolfe, Debbie M Hunt, Christina Kahramanoglou, Yvonne Braun, Angela Rodgers, Alix Blockley, Stephen Coade, Kathryn E A Lougheed, Nor Azian Hafneh, Sarah M Glenn, Jason C Crack, Nick E Le Brun, José W Saldanha, Vadim Makarov, Irene Nobeli, Kristine Arnvig, Galina V Mukamolova, Roger S Buxton, Jeffrey Green
Mycobacterium tuberculosis (MTb) is the causative agent of pulmonary tuberculosis (TB). MTb colonizes the human lung, often entering a non-replicating state before progressing to life-threatening active infections. Transcriptional reprogramming is essential for TB pathogenesis. In vitro, Cmr (a member of the CRP/FNR super-family of transcription regulators) bound at a single DNA site to act as a dual regulator of cmr transcription and an activator of the divergent rv1676 gene. Transcriptional profiling and DNA-binding assays suggested that Cmr directly represses dosR expression...
May 8, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28469152/lineage-specific-snps-for-genotyping-of-mycobacterium-tuberculosis-clinical-isolates
#19
Horng-Yunn Dou, Chien-Hsing Lin, Yih-Yuan Chen, Shiu-Ju Yang, Jia-Ru Chang, Keh-Ming Wu, Ying-Tsong Chen, Pei-Ju Chin, Yen-Ming Liu, Ih-Jen Su, Shih-Feng Tsai
Tuberculosis (TB) is a severe infectious disease worldwide. Genetic variation of the causative agent, Mycobacterium tuberculosis (MTB), determines the outcomes of infection and anti-TB treatment. Until recently, there has been no effective and convenient way for classifying clinical isolates based on the DNA sequences of the divergent lineages of MTB infecting human populations. Here, we identified single nucleotide polymorphisms (SNPs) of six representative strains from Taiwan by whole-genome sequencing and comparing the results to the sequence of the H37Rv reference strain...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28461970/proteomics-for-the-investigation-of-mycobacteria
#20
J A Bespyatykh, E A Shitikov, E N Ilina
The physiology of Mycobacterium tuberculosis, the causative agent of tuberculosis, is being studied with intensity. However, despite the genomic and transcriptomic data available today, the pathogenic potential of these bacteria remains poorly understood. Therefore, proteomic approaches seem relevant in studying mycobacteria. This review covers the main stages in the proteomic analysis methods used to study mycobacteria. The main achievements in the area of M. tuberculosis proteomics are described in general...
January 2017: Acta Naturae
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