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Mycobacterium tuberculosis Beijing

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https://www.readbyqxmd.com/read/28527287/-identification-of-the-mycobacterium-tuberculosis-beijing-lineage-in-ecuador
#1
Patricia Jiménez, Karina Calvopiña, Diana Herrera, Carlos Rojas, Laura Pérez-Lago, Marcelo Grijalva, Remedios Guna, Darío García-de Viedma
INTRODUCTION: Mycobacterium tuberculosis Beijing lineage isolates are considered to be especially virulent, transmissible and prone to acquire resistances. Beijing strains have been reported worldwide, but studies in Latin America are still scarce. The only multinational study performed in the region indicated a heterogeneous distribution for this lineage, which was absent in Chile, Colombia and Ecuador, although further studies found the lineage in Chile and Colombia. OBJECTIVE: To search for the presence of the Beijing lineage in Ecuador, the only country in the region where it remains unreported...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28522873/recombinant-bcg-expressing-ltak63-adjuvant-induces-superior-protection-against-mycobacterium-tuberculosis
#2
Ivan P Nascimento, Dunia Rodriguez, Carina C Santos, Eduardo P Amaral, Henrique K Rofatto, Ana P Junqueira-Kipnis, Eduardo D C Gonçalves, Maria R D'Império-Lima, Mario H Hirata, Celio L Silva, Nathalie Winter, Brigitte Gicquel, Kingston H G Mills, Mariagrazia Pizza, Rino Rappuoli, Luciana C C Leite
In order to develop an improved BCG vaccine against tuberculosis we have taken advantage of the adjuvant properties of a non-toxic derivative of Escherichia coli heat labile enterotoxin (LT), LTAK63. We have constructed rBCG strains expressing LTAK63 at different expression levels. Mice immunized with BCG expressing low levels of LTAK63 (rBCG-LTAK63lo) showed higher Th1 cytokines and IL-17 in the lungs, and when challenged intratracheally with Mycobacterium tuberculosis displayed a 2.0-3.0 log reduction in CFU as compared to wild type BCG...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28506215/comparison-of-antibody-responses-against-mycobacterium-tuberculosis-antigen-rv0679c-in-tuberculosis-patients-from-the-endemic-and-non-endemic-regions-of-the-beijing-genotype-a-case-control-study
#3
Jingge Zhao, Takashi Matsuba, Xiaoyan Zhang, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Elizabeth Freda Telan, Yasuhiko Suzuki, Toshio Hattori
BACKGROUND: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses...
May 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28482417/-molecular-epidemiologic-study-on-mycobacterium-tuberculosis-from-drug-resistance-monitoring-sites-of-guangdong-province-2015
#4
X C Huang, H X Guo, Z H Wu, C X Guo, W J Wei, H C Li, Q Sun, C C Zhang, Z Y Li, T Chen, Q Zhong, L Zhou
Objective: To understand the characteristics of Mycobacterium tuberculosis (MTB) in epidemiology and distribution from Guangdong Province, and to explore the risk factors associated with drug resistance. Methods: A total of 225 clinical strains of MTB collected from 5 drug resistance monitoring sites of Guangdong Province in 2015 were tested by Regions of Difference 105 (RD105) deletion test and 15 loci mycobacterial interspersed repetitive units (MIRU) were used for genotyping. Gene clustering was analyzed using BioNumerics7...
May 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28482027/cmr-is-a-redox-responsive-regulator-of-dosr-that-contributes-to-m-tuberculosis-virulence
#5
Laura J Smith, Aleksandra Bochkareva, Matthew D Rolfe, Debbie M Hunt, Christina Kahramanoglou, Yvonne Braun, Angela Rodgers, Alix Blockley, Stephen Coade, Kathryn E A Lougheed, Nor Azian Hafneh, Sarah M Glenn, Jason C Crack, Nick E Le Brun, José W Saldanha, Vadim Makarov, Irene Nobeli, Kristine Arnvig, Galina V Mukamolova, Roger S Buxton, Jeffrey Green
Mycobacterium tuberculosis (MTb) is the causative agent of pulmonary tuberculosis (TB). MTb colonizes the human lung, often entering a non-replicating state before progressing to life-threatening active infections. Transcriptional reprogramming is essential for TB pathogenesis. In vitro, Cmr (a member of the CRP/FNR super-family of transcription regulators) bound at a single DNA site to act as a dual regulator of cmr transcription and an activator of the divergent rv1676 gene. Transcriptional profiling and DNA-binding assays suggested that Cmr directly represses dosR expression...
May 8, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28469152/lineage-specific-snps-for-genotyping-of-mycobacterium-tuberculosis-clinical-isolates
#6
Horng-Yunn Dou, Chien-Hsing Lin, Yih-Yuan Chen, Shiu-Ju Yang, Jia-Ru Chang, Keh-Ming Wu, Ying-Tsong Chen, Pei-Ju Chin, Yen-Ming Liu, Ih-Jen Su, Shih-Feng Tsai
Tuberculosis (TB) is a severe infectious disease worldwide. Genetic variation of the causative agent, Mycobacterium tuberculosis (MTB), determines the outcomes of infection and anti-TB treatment. Until recently, there has been no effective and convenient way for classifying clinical isolates based on the DNA sequences of the divergent lineages of MTB infecting human populations. Here, we identified single nucleotide polymorphisms (SNPs) of six representative strains from Taiwan by whole-genome sequencing and comparing the results to the sequence of the H37Rv reference strain...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28461970/proteomics-for-the-investigation-of-mycobacteria
#7
J A Bespyatykh, E A Shitikov, E N Ilina
The physiology of Mycobacterium tuberculosis, the causative agent of tuberculosis, is being studied with intensity. However, despite the genomic and transcriptomic data available today, the pathogenic potential of these bacteria remains poorly understood. Therefore, proteomic approaches seem relevant in studying mycobacteria. This review covers the main stages in the proteomic analysis methods used to study mycobacteria. The main achievements in the area of M. tuberculosis proteomics are described in general...
January 2017: Acta Naturae
https://www.readbyqxmd.com/read/28456705/positive-epistasis-of-major-low-cost-drug-resistance-mutations-rpob531-ttg-and-katg315-acc-depends-on-the-phylogenetic-background-of-mycobacterium-tuberculosis-strains
#8
Qin-Jing Li, Wei-Wei Jiao, Qing-Qin Yin, Ying-Jia Li, Jie-Qiong Li, Fang Xu, Lin Sun, Jing Xiao, Hui Qi, Ting Wang, Igor Mokrousov, Hai-Rong Huang, A-Dong Shen
Mycobacterium tuberculosis Beijing genotype strains increasingly circulate in different world regions, either as historical endemic, e.g. in East Asia, or recently imported, e.g. in South America, and this family is regarded as the most successful lineage of the global tuberculosis (TB) epidemic. Here we analysed the transmission capacity of these strains in the context of their phylogenetic background and drug resistance mutations. The study collection included all multidrug resistant (MDR) strains of Beijing genotype isolated in Beijing Chest Hospital, the largest tertiary TB facility in North China, in 2011-2013 (n = 278)...
June 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28446574/the-use-of-genexpert-remnants-for-drug-resistance-profiling-and-molecular-epidemiology-of-tuberculosis-in-libreville-gabon
#9
Amel Kévin Alame-Emane, Catherine Pierre-Audigier, Oriane Cordelia Aboumegone-Biyogo, Amandine Nzoghe-Mveang, Véronique Cadet-Daniel, Christophe Sola, Joël Fleury Djoba-Siawaya, Brigitte Gicquel, Howard E Takiff
Multidrug (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis are major problems in global health. The GeneXpertMTB/RIF (Xpert) rapidly detects resistance to rifampicin (RIF-R), but detection of the additional resistance that defines MDR and XDR-TB, and for molecular epidemiology, specimen cultures and biosafe infrastructure are generally required. We sought to determine whether the remnants of sputa prepared for Xpert could be used directly to find mutations associated with drug resistance and for molecular epidemiology, and thus provide a precise characterization of MDR-TB cases in countries lacking BSL3 facilities for M...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28438994/latently-and-uninfected-healthcare-workers-exposed-to-tb-make-protective-antibodies-against-mycobacterium-tuberculosis
#10
Hao Li, Xing-Xing Wang, Bin Wang, Lei Fu, Guan Liu, Yu Lu, Min Cao, Hairong Huang, Babak Javid
The role of Igs in natural protection against infection by Mycobacterium tuberculosis (Mtb), the causative agent of TB, is controversial. Although passive immunization with mAbs generated against mycobacterial antigens has shown protective efficacy in murine models of infection, studies in B cell-depleted animals only showed modest phenotypes. We do not know if humans make protective antibody responses. Here, we investigated whether healthcare workers in a Beijing TB hospital-who, although exposed to suprainfectious doses of pathogenic Mtb, remain healthy-make antibody responses that are effective in protecting against infection by Mtb...
May 9, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28416956/mutations-in-streptomycin-resistance-genes-and-their-relationship-to-streptomycin-resistance-and-lineage-of-mycobacterium-tuberculosis-thai-isolates
#11
Yin Moe Hlaing, Pongsri Tongtawe, Pramuan Tapchaisri, Jeeraphong Thanongsaksrikul, Unchana Thawornwan, Buppa Archanachan, Potjanee Srimanote
BACKGROUND: Streptomycin (SM) is recommended by the World Health Organization (WHO) as a part of standard regimens for retreating multidrug-resistant tuberculosis (MDR-TB) cases. The incidence of MDR-TB in retreatment cases was 19% in Thailand. To date, information on SM resistance (SMR) gene mutations correlated to the SMR of Mycobacterium tuberculosis Thai isolates is limited. In this study, the mutations in rpsL, rrs, gidB, and whiB7 were investigated and their association to SMR and the lineage of M...
April 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28411454/synthesis-and-immunogenicity-of-pg-tb1-monovalent-glycoconjugate
#12
Xin Meng, Chuanming Ji, Chao Su, Di Shen, Yaxin Li, Peijie Dong, Ding Yuan, Mengya Yang, Song Bai, Demei Meng, Zhenchuan Fan, Yang Yang, Peng Yu, Tao Zhu
A PG-tb1 hapten from the West Beijing strains of Mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to CRM197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent T-dependent conjugate vaccine. The intermediate monoester can be easily purified and the degree of incorporation can be monitored by MALDI-TOF mass spectrometry. After administered systemically in mice without any adjuvant, the conjugate induced high antigen-specific IgG levels in serum...
March 27, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28404234/frequency-of-rrs-and-rpsl-mutations-in-streptomycin-resistant-mycobacterium-tuberculosis-isolates-from-iranian-patients
#13
Azar Dokht Khosravi, Nayereh Etemad, Mohammad Hashemzadeh, Solmaz Khandan Dezfuli, Hamed Goodarzi
OBJECTIVES: Streptomycin (SM) is one of the most effective drugs for the treatment of multidrug-resistant (MDR) tuberculosis. However, resistance to SM is increasingly reported, mainly due to mutations in the rpsL and rrs genes. This study was designed with the aim of determining the nature of SM resistance and the type and frequency of rpsL and rrs mutations among SM-resistant Mycobacterium tuberculosis (MTB) isolates from Iran. METHODS: A total of 100 clinical monoresistant and MDR MTB isolates were subjected to drug susceptibility testing (DST) for SM...
April 9, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28397531/drug-resistance-in-newly-presenting-and-previously-treated-tuberculosis-patients-in-guangxi-province-people-s-republic-of-china
#14
Dan Luo, Jinming Zhao, Mei Lin, Feiying Liu, Shuhai Huang, Yingkun Zhang, Minying Huang, Juan Li, Yang Zhou, Rushu Lan, Yanlin Zhao
Drug-resistant Mycobacterium tuberculosis strains are a major threat to the control of tuberculosis (TB), but the prevalence of drug-resistant TB is still unknown in the southern ethnic region of China. A cluster-randomized sampling method was used to include the study population. Isolates were tested for resistance to 6 antituberculosis drugs, and genotyped to identify Beijing strains. Overall, 11.3% (139/1229) of new cases and 33.0% (126/382) of retreated cases had drug-resistant tuberculosis. Multiple previous TB treatment episodes and multiple treatment interruptions were risk factors for both drug-resistant and multidrug-resistant TB among retreated cases...
April 1, 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28394899/genomic-analyses-of-the-ancestral-manila-family-of-mycobacterium-tuberculosis
#15
Xuehua Wan, Kent Koster, Lishi Qian, Edward Desmond, Richard Brostrom, Shaobin Hou, James T Douglas
With its airborne transmission and prolonged latency period, Mycobacterium tuberculosis spreads worldwide as one of the most successful bacterial pathogens and continues to kill millions of people every year. M. tuberculosis lineage 1 is inferred to originate ancestrally based on the presence of the 52-bp TbD1 sequence and analysis of single nucleotide polymorphisms. Previously, we briefly reported the complete genome sequencing of M. tuberculosis strains 96121 and 96075, which belong to the ancient Manila family and modern Beijing family respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28377903/the-bioinformatics-analysis-of-comparative-genomics-of-mycobacterium-tuberculosis-complex-mtbc-provides-insight-into-dissimilarities-between-intraspecific-groups-differing-in-host-association-virulence-and-epitope-diversity
#16
Xinmiao Jia, Li Yang, Mengxing Dong, Suting Chen, Lingna Lv, Dandan Cao, Jing Fu, Tingting Yang, Ju Zhang, Xiangli Zhang, Yuanyuan Shang, Guirong Wang, Yongjie Sheng, Hairong Huang, Fei Chen
Tuberculosis now exceeds HIV as the top infectious disease cause of mortality, and is caused by the Mycobacterium tuberculosis complex (MTBC). MTBC strains have highly conserved genome sequences (similarity >99%) but dramatically different phenotypes. To analyze the relationship between genotype and phenotype, we conducted the comparative genomic analysis on 12 MTBC strains representing different lineages (i.e., Mycobacterium bovis; M. bovis BCG; M. microti; M. africanum; M. tuberculosis H37Rv; M. tuberculosis H37Ra, and six M...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28374900/spoligotyping-of-mycobacterium-tuberculosis-isolates-at-a-tertiary-care-hospital-in-india
#17
Shirly Suzana, Sivakumar Shanmugam, K R Uma Devi, P N Swarna Latha, Joy S Michael
OBJECTIVE: Spoligotyping is a valuable genotyping tool to study the genetic diversity and molecular epidemiology of Mycobacterium tuberculosis (M. tb). The aim of this study was to analyse different spoligotype patterns of M. tb strains isolated from patients with tuberculosis from different parts of India. MATERIALS AND METHODS: A total of 163 M. tb isolates were spoligotyped between January 2014 and January 2015. About 47% (n = 77) were from patients with extrapulmonary tuberculosis; of these, 10 were MDR, and seven were Pre-XDR...
April 4, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28338924/a-nonsynonymous-snp-catalog-of-mycobacterium-tuberculosis-virulence-genes-and-its-use-for-detecting-new-potentially-virulent-sublineages
#18
Natalya E Mikheecheva, Marina V Zaychikova, Alexander V Melerzanov, Valery N Danilenko
Mycobacterium tuberculosis is divided into several distinct lineages, and various genetic markers such as IS-elements, VNTR, and SNPs are used for lineage identification. We propose an M. tuberculosis classification approach based on functional polymorphisms in virulence genes. An M. tuberculosis virulence genes catalog has been established, including 319 genes from various protein groups, such as proteases, cell wall proteins, fatty acid and lipid metabolism proteins, sigma factors, toxin-antitoxin systems...
April 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28337447/genotypic-diversity-of-mycobacterium-tuberculosis-clinical-isolates-in-the-multiethnic-area-of-the-xinjiang-uygur-autonomous-region-in-china
#19
Jie Liu, Junlian Li, Jiao Liu, Xiuqin Zhao, Lulu Lian, Haican Liu, Bing Lu, Qin Yu, Jingrui Zhang, Yingcheng Qi, Kanglin Wan
Objectives. We studied the genetic diversity of clinical isolates from patients with tuberculosis in the multiethnic area of Xinjiang autonomous region in China. A total of 311 clinical M. tuberculosis isolates were collected in 2006 and 2011 and genotyped by two genotyping methods. All isolates were grouped into 68 distinct spoligotypes using the spoligotyping method. The Beijing family was dominant, followed by T1 and CAS. MIRU-VNTR results showed that a total of 195 different VNTR types were identified. Ten of the 15 loci were highly or moderately discriminant according to their HGDI scores, and 13 loci had good discriminatory power in non-Beijing family strains, whereas only two loci had good discriminatory power in Beijing family strains...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28333978/association-between-genotype-and-drug-resistance-profiles-of-mycobacterium-tuberculosis-strains-circulating-in-china-in-a-national-drug-resistance-survey
#20
Yang Zhou, Susan van den Hof, Shengfen Wang, Yu Pang, Bing Zhao, Hui Xia, Richard Anthony, Xichao Ou, Qiang Li, Yang Zheng, Yuanyuan Song, Yanlin Zhao, Dick van Soolingen
We describe the population structure of a representative collection of 3,133 Mycobacterium tuberculosis isolates, collected within the framework of a national resistance survey from 2007 in China. Genotyping data indicate that the epidemic strains in China can be divided into seven major complexes, of which 92% belonged to the East Asian (mainly Beijing strains) or the Euro-American lineage. The epidemic Beijing strains in China are closely related to the Beijing B0/W148 strain earlier described in Russia and a large cluster of these strains has spread national wide...
2017: PloS One
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