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https://www.readbyqxmd.com/read/28644970/overexpression-of-populus%C3%A3-canescens-isoprene-synthase-gene-in-camelina-sativa-leads-to-alterations-in-its-growth-and-metabolism
#1
Lorenzo Rossi, Monica Borghi, Jinfen Yang, De-Yu Xie
Isoprene (2-methyl-1,3-butadiene) is a hemiterpene molecule. It has been estimated that the plant kingdom emits 500-750 million tons of isoprene in the environment, half of which results from tropical broadleaf trees and the remainder from shrubs. Camelina (Camelina sativa (L.) Crantz) is an emerging bioenergy plant for biodiesel. In this study, we characterized isoprene formation following a diurnal/nocturnal cycle in wild-type Camelina plants. To understand the potential effects of isoprene emission on this herbaceous plant, a gray poplar Populus×canescens isoprene synthase gene (PcISPS) was overexpressed in Camelina...
June 13, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28644883/effects-of-dietary-inclusion-of-high-concentrations-of-crude-glycerin-on-meat-quality-and-fatty-acid-profile-of-feedlot-fed-nellore-bulls
#2
Eric H C B van Cleef, André P D'Áurea, Vanessa R Fávaro, Flavia O S van Cleef, Robson S Barducci, Marco T C Almeida, Otávio R Machado Neto, Jane M B Ezequiel
Crude glycerin, the main by-product of biodiesel production, can replace dietary energy sources, such as corn. The objective of this study was to evaluate the inclusion of up to 30% of crude glycerin in dry matter (DM) of the total diets, and its effects on meat quality parameters of feedlot Nellore bulls. Thirty animals (227.7 ± 23.8 kg body weight; 18 months old) were housed in individual pens and fed 5 experimental diets, containing 0, 7.5, 15, 22.5 or 30% crude glycerin (DM basis). After 103 d (21 d adaptation) animals were slaughtered and the Longissimus muscle was collected...
2017: PloS One
https://www.readbyqxmd.com/read/28642053/from-low-cost-substrates-to-single-cell-oils-synthesized-by-oleaginous-yeasts
#3
REVIEW
Lei Qin, Lu Liu, An-Ping Zeng, Dong Wei
As new feedstock for biofuels, microbial oils have received worldwide attentions due to their environmentally-friendly characters. Microbial oil production based on low-cost raw materials is significantly attractive to the current biodiesel refinery industry. In terms of SCOs production, oleaginous yeast has numerous advantages over bacteria, molds and microalgae based on their high growth rate and lipid yield. Numerous efforts have been made on the competitive lipid production combining the use of cheap raw materials as substrates by yeasts...
May 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28639534/synthesis-of-bioadditives-of-fuels-from-biodiesel-derived-glycerol-by-esterification-with-acetic-acid-on-solid-catalysts
#4
Gabriel A Bedogni, Mauro D Acevedo, Federico Aguzín, Nora B Okulik, Cristina L Padró
In this paper, glycerol esterification with acetic acid was studied on several solid acid catalysts: Al2O3, Al-MCM-41, HPA/SiO2, HBEA, Amberlyst-15 and Amberlyst-36 with the aim of determining the reaction conditions and the nature of the surface acid sites required to produce selectively triacetylglycerol (triacetin). The acidity of the catalysts (nature, density and strength of acid sites) was characterized by temperature programmed desorption of NH3 and FTIR of adsorbed pyridine. Al2O3 (Lewis acidity) did not show any activity in the reaction...
June 22, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28639019/cultivation-of-freshwater-microalgae-in-biodiesel-wash-water
#5
Patrícia Giulianna Petraglia Sassi, Clediana Dantas Calixto, Jordana Kaline da Silva Santana, Roberto Sassi, Cristiane Francisca Costa Sassi, Raphael Abrahão
Biodiesel wash water is a contaminating industrial effluent that must be treated prior to disposal. The use of this effluent as a low-cost alternative cultivation medium for microalgae could represent a viable supplementary treatment. We cultivated 11 microalgae species with potential use for biodiesel production to assess their growth capacities in biodiesel industrial washing waters. Only Monoraphidium contortum, Ankistrodesmus sp., Chlorococcum sp., and one unidentified Chlorophyceae species grew effectively in that effluent...
June 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28634846/lipid-accumulation-and-metabolic-analysis-based-on-transcriptome-sequencing-of-filamentous-oleaginous-microalgae-tribonema-minus-at-different-growth-phases
#6
Hui Wang, Lili Gao, Huimin Shao, Wenjun Zhou, Tianzhong Liu
Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase...
June 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28628983/immobilized-oleaginous-microalgae-for-production-of-lipid-and-phytoremediation-of-secondary-effluent-from-palm-oil-mill-in-fluidized-bed-photobioreactor
#7
Benjamas Cheirsilp, Tipawan Thawechai, Poonsuk Prasertsan
Oleaginous microalga Nannochloropsis sp. was immobilized in alginate gel beads and cultivated under optimal conditions that their growth and lipid production were comparable to those of free cells. The immobilized cells were used in phytoremediation of secondary effluent from palm oil mill and easily recovered by simple sieving method. The immobilized cells contributed to removal of nitrogen and phosphorus >90% and CO2 mitigation >99%. They also gave the biomass and lipid production of 1.300±0.050g/L and 0...
June 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628981/biodiesel-synthesis-from-calophyllum-inophyllum-oil-with-different-supercritical-fluids
#8
Neha Lamba, Kaveri Gupta, Jayant M Modak, Giridhar Madras
Biodiesel or fatty acid methyl esters (FAMEs) is primarily synthesized using edible vegetable oils and methanol with a catalyst. However, in the present study, FAMEs were synthesized from a non-edible oil (Calophyllum inophyllum also called as sura honne, Punnagam, Alexandrian Laurel) in different supercritical fluids: methanol (MeOH), methyl tert-butyl ether (MTBE), methyl acetate (MeOAc) and dimethyl carbonate (DMC) non-catalytically. Reactions were performed from 523K to 673K at 30MPa with a molar ratio of 40:1 with times varying from 3min to 3h...
June 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28624246/uv-c-irradiation-accelerates-neutral-lipid-synthesis-in-the-marine-oleaginous-diatom-fistulifera-solaris
#9
Atsushi Arakaki, Takuya Matsumoto, Takuma Tateishi, Mitsufumi Matsumoto, Daisuke Nojima, Yoshino Tomoko, Tsuyoshi Tanaka
This study investigated the induction of oil synthesis in the oleaginous diatom, Fistulifera solaris, following irradiation with small doses of UV-C. A rapid induction of oil accumulation was confirmed within 6h following UV-C radiation of the diatom cells, with increases in cell oil body volumes after 24h of approximately 4- to 6-fold from the initial volume. Reactive oxygen species (ROS), which can be generated by a UV-C-mediated reaction, were detected in irradiated cells and the correlation between ROS generation and oil accumulation was confirmed...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28623567/molecular-identification-and-comparative-evaluation-of-tropical-marine-microalgae-for-biodiesel-production
#10
Sanyo Sabu, I S Bright Singh, Valsamma Joseph
Marine microalgae have emerged as important feedstock for liquid biofuel production. The identification of lipid-rich native microalgal species with high growth rate and optimal fatty acid profile and biodiesel properties is the most challenging step in microalgae-based biodiesel production. In this study, attempts have been made to bio-prospect the biodiesel production potential of marine and brackish water microalgal isolates from the west coast of India. A total of 14 microalgal species were isolated, identified using specific molecular markers and based on the lipid content; seven species with total lipid content above 20% of dry cell weight were selected for assessing biodiesel production potential in terms of lipid and biomass productivities, nile red fluorescence, fatty acid profile and biodiesel properties...
June 16, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28623565/the-novel-oleaginous-bacterium-sphingomonas-sp-egy1-dsm-29616-a-value-added-platform-for-renewable-biodiesel
#11
Nehad N Amer, Yasser Elbahloul, Amira M Embaby, Ahmed Hussein
Oleaginous microorganisms are regarded as efficient, renewable cell factories for lipid biosynthesis, a biodiesel precursor, to overwhelm the cosmopolitan energy crisis with affordable investment capital costs. Present research highlights production and characterization of lipids by a newly isolated oleaginous bacterium, Sphingomonas sp. EGY1 DSM 29616 through an eco-friendly approach. Only sweet whey [42.1% (v/v)] in tap water was efficiently used as a growth medium and lipid production medium to encourage cell growth and trigger lipid accumulation simultaneously...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28622654/continuous-production-of-biodiesel-under-supercritical-methyl-acetate-conditions-experimental-investigation-and-kinetic-model
#12
Obie Farobie, Yukihiko Matsumura
In this study, biodiesel production by using supercritical methyl acetate in a continuous flow reactor was investigated for the first time. The aim of this study was to elucidate the reaction kinetics of biodiesel production by using supercritical methyl. Experiments were conducted at various reaction temperatures (300-400°C), residence times (5-30min), oil-to-methyl acetate molar ratio of 1:40, and a fixed pressure of 20MPa. Reaction kinetics of biodiesel production with supercritical methyl acetate was determined...
June 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28620618/histopathological-and-reproductive-evaluation-in-male-rats-fed-jatropha-curcas-seed-cake-with-or-without-alkaline-hydrolysis-and-subjected-to-heat-treatment
#13
Laiane Teixeira Sousa Moura, Domenica Palomaris Mariano Souza, Simone Mendonça, José Antônio de Aquino Ribeiro, Luciano Fernandes Sousa, Adriano Tony Ramos, Paulo César Maiorka, Vera Lúcia de Araújo, Viviane Mayumi Maruo
Jatropha curcas cake, a by-product of biodiesel production, is rich in protein and has potential to be used in livestock feed; however, the presence of antinutritional factors and phorbol esters limits its use. Thus, this study investigated toxicological and reproductive effects in male Wistar rats after subchronic exposure to J. curcas cake subjected to detoxification procedures. Rats were divided into seven groups (n = 10) and treated for 60 days. The control group received commercial feed, while experimental groups received a diet containing 5% J...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28616071/combined-de-novo-and-ex-novo-lipid-fermentation-in-a-mix-medium-of-corncob-acid-hydrolysate-and-soybean-oil-by-trichosporon-dermatis
#14
Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Gao-Xiang Qi, Lian Xiong, Xiao-Qing Lin, Can Wang, Hai-Long Li, Xin-De Chen
BACKGROUND: Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and hydrophobic substrates can be utilized by oleaginous microorganisms with two different bio-pathways ("de novo" lipid fermentation and "ex novo" lipid fermentation). To date, most of the research on lipid fermentation has focused mainly on only one fermentation pathway and little work was carried out on both "de novo" and "ex novo" lipid fermentation simultaneously; thus, the advantages of both lipid fermentation cannot be fulfilled comprehensively...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28603295/unsupervised-classification-of-petroleum-certified-reference-materials-and-other-fuels-by-chemometric-analysis-of-gas-chromatography-mass-spectrometry-data
#15
Werickson Fortunato de Carvalho Rocha, Michele M Schantz, David A Sheen, Pamela M Chu, Katrice A Lippa
As feedstocks transition from conventional oil to unconventional petroleum sources and biomass, it will be necessary to determine whether a particular fuel or fuel blend is suitable for use in engines. Certifying a fuel as safe for use is time-consuming and expensive and must be performed for each new fuel. In principle, suitability of a fuel should be completely determined by its chemical composition. This composition can be probed through use of detailed analytical techniques such as gas chromatography-mass spectroscopy (GC-MS)...
June 1, 2017: Fuel
https://www.readbyqxmd.com/read/28602663/establishing-a-green-platform-for-biodiesel-synthesis-via-strategic-utilization-of-biochar-and-dimethyl-carbonate
#16
Jechan Lee, Jong-Min Jung, Jeong-Ik Oh, Yong Sik Ok, Eilhann E Kwon
To establish a green platform for biodiesel production, this study mainly investigates pseudo-catalytic (non-catalytic) transesterification of olive oil. To this end, biochar from agricultural waste (maize residue) and dimethyl carbonate (DMC) as an acyl acceptor were used for pseudo-catalytic transesterification reaction. Reaction parameters (temperature and molar ratio of DMC to olive oil) were also optimized. The biodiesel yield reached up to 95.4% under the optimal operational conditions (380°C and molar ratio of DMC to olive oil (36:1))...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28602187/desi-ms-imaging-and-nmr-spectroscopy-to-investigate-the-influence-of-biodiesel-in-the-structure-of-commercial-rubbers
#17
Lorena M A Silva, Elenilson G Alves Filho, André J Simpson, Marcos R Monteiro, Elaine Cabral, Demian Ifa, Tiago Venâncio
Biodiesel has been introduced as an energetic matrix in several countries around the world. However, the affinity of biodiesel with the components of petrodiesel engines is a growing concern. In order to obtain information regarding the effect of biodiesel on the rubber structure, nuclear magnetic resonance technics under a new technology named as comprehensive multiphase (CMP NMR) and the imaging through desorption electrospray ionization mass spectrometry (DESI-MS imaging) were used. The (1)H CMP-DOSY NMR showed the entrapped fuel into the rubber cavities, which the higher constraint caused by the rubber structure is related to the smaller diffusion coefficient...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28601770/bio-hythane-production-from-microalgae-biomass-key-challenges-and-potential-opportunities-for-algal-bio-refineries
#18
Anish Ghimire, Gopalakrishnan Kumar, Periyasamy Sivagurunathan, Sutha Shobana, Ganesh D Saratale, Hyun Woo Kim, Vincenzo Luongo, Giovanni Esposito, Raul Munoz
The interest in microalgae for wastewater treatment and liquid bio-fuels production (i.e. biodiesel and bioethanol) is steadily increasing due to the energy demand of the ultra-modern technological world. The associated biomass and by-product residues generated from these processes can be utilized as a feedstock in anaerobic fermentation for the production of gaseous bio-fuels. In this context, dark fermentation coupled with anaerobic digestion can be a potential technology for the production of hydrogen and methane from these residual algal biomasses...
May 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28599651/microtiter-plate-cultivation-of-oleaginous-fungi-and-monitoring-of-lipogenesis-by-high-throughput-ftir-spectroscopy
#19
Gergely Kosa, Achim Kohler, Valeria Tafintseva, Boris Zimmermann, Kristin Forfang, Nils Kristian Afseth, Dimitrios Tzimorotas, Kiira S Vuoristo, Svein Jarle Horn, Jerome Mounier, Volha Shapaval
BACKGROUND: Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production of omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) and have been suggested as an alternative route for biodiesel production. Initial research steps for various applications include the screening of fungi in order to find efficient fungal producers with desired fatty acid composition. Traditional cultivation methods (shake flask) and lipid analysis (extraction-gas chromatography) are not applicable for large-scale screening due to their low throughput and time-consuming analysis...
June 9, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28597157/optimization-of-cultivation-conditions-for-biotechnological-production-of-lipid-by-rhodotorula-kratochvilovae-syn-rhodosporidium-kratochvilovae-sy89-for-biodiesel-preparation
#20
Tamene Milkessa Jiru, Marizeth Groenewald, Carolina Pohl, Laurinda Steyn, Nicholas Kiggundu, Dawit Abate
Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89, an oleaginous yeast, isolated from Ethiopian soil, was grown under nitrogen-limited media. The capacity this with respect to biomass production, lipid yield and lipid content was evaluated. The influence of inoculum size, carbon sources, variations in glucose concentration, nitrogen sources, C/N ratio, pH, temperature, agitation, and aeration rate and incubation period were investigated. Inoculum size of 10% v/v, glucose as a carbon source at 50 g/L glucose, 0...
June 2017: 3 Biotech
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