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Vitor S Domingues, Andrea S Monteiro, Gabriella Freitas Ferreira, Vera L Santos
The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to pH and temperature variations and in the presence of some electrolytes were evaluated. The lipopolysaccharide was used in concentrations ranging from 20 to 80 mgL(-1) to flocculate 100 gL(-1) of kaolin and 50 gL(-1) of charcoal...
December 5, 2016: Applied Biochemistry and Biotechnology
Augusto Etchegaray, François Coutte, Gabrielle Chataigné, Max Béchet, Ramon H Z Dos Santos, Valérie Leclère, Philippe Jacques
Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth...
September 2, 2016: Canadian Journal of Microbiology
Marcin Podleśny, Piotr Jarocki, Jakub Wyrostek, Tomasz Czernecki, Jagoda Kucharska, Anna Nowak, Zdzisław Targoński
Succinic acid is an important C4-building chemical platform for many applications. A novel succinic acid-producing bacterial strain was isolated from goat rumen. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the genus Enterobacter. This is the first report of a wild bacterial strain from the genus Enterobacter that is capable of efficient succinic acid production. Co-fermentation of glycerol and lactose significantly improved glycerol utilization under anaerobic conditions, debottlenecking the utilization pathway of this valuable biodiesel waste product...
December 1, 2016: Microbial Biotechnology
Rajesh Kumar Sahoo, Mohit Kumar, Lala Behari Sukla, Enketeswara Subudhi
Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65 °C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75 °C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate...
November 28, 2016: Environmental Science and Pollution Research International
Ana Claver, Raquel Rey, M Victoria López, Rafael Picorel, Miguel Alfonso
We studied erucic acid accumulation in the biodiesel feedstock Pennycress (Thlaspi arvense L.) as a first step towards the development of a sustainable strategy for biofuel production in the EU territory. To that end, two inbred Pennycress lines of European origin, "NASC" and "French," were cultivated in a controlled chamber and in experimental field plots, and their growth, seed production and seed oil characteristics analyzed. Differences in some agronomical traits like vernalization (winter-French versus spring-NASC), flowering time (delayed in the French line) and seed production (higher in the French line) were detected...
November 18, 2016: Journal of Plant Physiology
Ga-Yeong Kim, Yeo-Myeong Yun, Hang-Sik Shin, Jong-In Han
This study investigated if an effluent from anaerobic digestion (AD) system can be used as a nutrients source for the microalgae cultivation, and in so doing, if the effluent can be properly treated. Nitrogen and phosphorus in the AD effluent well supported microalgal growth, and their removal efficiency reached >97.9% and 99.2%, respectively. Among four different algal species tested, Micractinium inermum particularly stood out, showing the highest biomass and FAME productivity: 0.16gL(-1)d(-1) with 3.23gL(-1) of dry cell weight, and 0...
November 16, 2016: Bioresource Technology
Hao Cao, Yang Jiang, Haiyang Zhang, Kaili Nie, Ming Lei, Li Deng, Fang Wang, Tianwei Tan
The methanol resistance of lipase is a critical parameter in enzymatic biodiesel production. In the present work, the methanol resistance of Yarrowia lipolytica Lipase 2 (YLLIP2) was significantly improved using β-cyclodextrin (β-CD) as an additive. According to the results, YLLIP2 with β-CD exhibited approximately 7000U/mg specific activity in 30wt% methanol for 60min compared with no activity without β-CD under the same conditions. Molecular dynamics (MD) simulation results indicated that the β-CD molecules weakened the conformational change of YLLIP2 and maintained a semi-open state of the lid by overcoming the interference caused by methanol molecules...
January 2017: Enzyme and Microbial Technology
Livia T A Souza, Adriano A Mendes, Heizir F de Castro
The present study deals with the enzymatic synthesis of biodiesel by transesterification of Jatropha oil (Jatropha curcas L.) with ethanol in a solvent-free system. Seven commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-polyvinyl alcohol composite (epoxy-SiO2-PVA) were tested as biocatalysts. Among them, immobilized lipases from Pseudomonas fluorescens (lipase AK) and Burkholderia cepacia (lipase PS) were the most active biocatalysts in biodiesel synthesis, reaching ethyl ester yields (FAEE) of 91...
2016: BioMed Research International
Sravan Kumar Yellapu, Rajwinder Kaur, Rajeshwar D Tyagi
In situ transesterification of oleaginous yeast wet biomass for fatty acid methyl esters (FAMEs) production using acid catalyst, methanol with or without N-Lauroyl sarcosine (N-LS) treatment was performed. The maximum FAMEs yield obtained with or without N-LS treatment in 24h reaction time was 96.1±1.9 and 71±1.4% w/w, respectively. The N-LS treatment of biomass followed by with or without ultrasonication revealed maximum FAMEs yield of 94.3±1.9% and 82.9±1.8% w/w using methanol to lipid molar ratio 360:1 and catalyst concentration 360mM (64μL H2SO4/g lipid) within 5 and 25min reaction time, respectively...
November 9, 2016: Bioresource Technology
Florencia Eberhardt, Andres Aguirre, Hugo G Menzella, Salvador Peiru
Biodiesels produced from transesterification of vegetable oils have a major problem in quality due to the presence of precipitates, which are mostly composed of steryl glucosides (SGs). We have recently described an enzymatic method for the efficient removal of SGs from biodiesel, based on the activity of a thermostable β-glycosidase from Thermococcus litoralis. In the present work, we describe the development of an Escherichia coli-based expression system and a high cell density fermentation process. Strain and process engineering include the assessment of different promoters to drive the expression of a codon-optimized gene, the co-expression of molecular chaperones and the development of a high cell density fermentation process...
November 19, 2016: Journal of Industrial Microbiology & Biotechnology
S T Gumbi, B M Majeke, A O Olaniran, T Mutanda
Exploring indigenous microalgae capable of producing significant amounts of neutral lipids through high-throughput screening is crucial for sustainable biodiesel production. In this study, 31 indigenous microalgal strains were isolated from diverse aquatic habitats in KwaZulu-Natal, South Africa. Eight superior lipid-producing strains were selected for further analysis, based on Nile red fluorescence microscopy screening. The microalgal isolates were identified to belong to the genera Chlorella, Neochloris and Chlamydomonas via morpho-taxonomic and molecular approach by 18S rRNA gene sequencing...
November 18, 2016: Applied Biochemistry and Biotechnology
Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
The presence of high levels of free fatty acids in oil is a barrier to one-step biodiesel production. Undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. This work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides. A partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic production of fatty acid methyl ester (FAME)...
November 17, 2016: Biotechnology Journal
Andrius Tamošiūnas, Pranas Valatkevičius, Dovilė Gimžauskaitė, Mejdi Jeguirim, Vladas Mėčius, Mindaugas Aikas
Glycerol, considered as a waste feedstock resulting from biodiesel production, has received much attention in recent years due to its properties, which offer to recover energy. The aim of this study was to investigate the use of a thermal water vapor plasma for waste (crude) glycerol conversion to synthesis gas, or syngas (H2 + CO). In parallel of crude glycerol, a pure glycerol (99.5%) was used as a reference material in order to compare the concentrations of the formed product gas. A direct current (DC) arc plasma torch stabilized by a mixture of argon/water vapor was utilized for the effective glycerol conversion to hydrogen-rich synthesis gas...
November 17, 2016: Environmental Science and Pollution Research International
Qiang Liu, Youping Sun, Jinzheng Chen, Peiwang Li, Changzhu Li, Genhua Niu, Lijuan Jiang
BACKGROUND: Symplocos paniculata, asiatic sweetleaf or sapphire berry, is a widespread shrub or small tree from Symplocaceae with high oil content and excellent fatty acid composition in fruit. It has been used as feedstocks for biodiesel and cooking oil production in China. Little transcriptome information is available on the regulatory molecular mechanism of oil accumulation at different fruit development stages. RESULTS: The transcriptome at four different stages of fruit development (10, 80,140, and 170 days after flowering) of S...
November 16, 2016: BMC Genomics
Basavaraj Palabhanvi, Muthusivaramapandian Muthuraj, Vikram Kumar, Mayurketan Mukherjee, Saumya Ahlawat, Debasish Das
A novel two-stage continuous heterotrophic cultivation of Chlorella sp. FC2 IITG was demonstrated for enhanced lipid productivity. Initially, effect of control variable e.g. dilution rate and feed stream substrate concentrations on biomass productivity was evaluated. This showed significant variation in biomass productivity from 2.4gL(-1)day(-1) to 11.2gL(-1)day(-1). Further, these control variables were optimized by using multi-nutrient mechanistic model for maximizing the biomass productivity. Finally, continuous production of lipid rich algal biomass was demonstrated in two sequential bioreactors for enhanced lipid productivity...
November 9, 2016: Bioresource Technology
Yong Zhang, Yunjian Jiang
Waste cooking oil (WCO)-for-biodiesel conversion is regarded as the "waste-to-wealthy" industry. This paper addresses the design of a WCO-for-biodiesel supply chain at both strategic and tactical levels. The supply chain of this problem is studied, which is based on a typical mode of the waste collection (from restaurants' kitchen) and conversion in the cities. The supply chain comprises three stakeholders: WCO supplier, integrated bio-refinery and demand zone. Three key problems should be addressed for the optimal design of the supply chain: (1) the number, sizes and locations of bio-refinery; (2) the sites and amount of WCO collected; (3) the transportation plans of WCO and biodiesel...
November 12, 2016: Waste Management
Baoshan Guo, Cheng Lei, Takuro Ito, Yiyue Jiang, Yasuyuki Ozeki, Keisuke Goda
The development of reliable, sustainable, and economical sources of alternative fuels is an important, but challenging goal for the world. As an alternative to liquid fossil fuels, algal biofuel is expected to play a key role in alleviating global warming since algae absorb atmospheric CO2 via photosynthesis. Among various algae for fuel production, Euglena gracilis is an attractive microalgal species as it is known to produce wax ester (good for biodiesel and aviation fuel) within lipid droplets. To date, while there exist many techniques for inducing microalgal cells to produce and accumulate lipid with high efficiency, few analytical methods are available for characterizing a population of such lipid-accumulated microalgae including E...
2016: PloS One
Helena Libalova, Pavel Rossner, Kristyna Vrbova, Tana Brzicova, Jitka Sikorova, Michal Vojtisek-Lom, Vit Beranek, Jiri Klema, Miroslav Ciganek, Jiri Neca, Katerina Pencikova, Miroslav Machala, Jan Topinka
This study used toxicogenomics to identify the complex biological response of human lung BEAS-2B cells treated with organic components of particulate matter in the exhaust of a diesel engine. First, we characterized particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in its neat form (B100) and 30% by volume blend with diesel fuel (B30), and neat hydrotreated vegetable oil (NEXBTL100). The concentration of polycyclic aromatic hydrocarbons (PAHs) and their derivatives in organic extracts was the lowest for NEXBTL100 and higher for biodiesel...
November 3, 2016: International Journal of Molecular Sciences
Juntao Xu, Changsheng Liu, Meng Wang, Lei Shao, Li Deng, Kaili Nie, Fang Wang
The aim of the present work was to study the applicability of rotating packed bed (RPB) for biodiesel through the biocatalytic method. In this research, the RPB facilitated a more homogeneous mixture of substrates due to its higher mass transfer efficiency and better micromixing environment. This was superior to the traditional continuous stirred tank reactor (CSTR) system. Candida sp. 99-125 lipase was used without any organic solvent or additive, and demonstrated a significant catalyst efficiency. The key factors, such as the high gravity factor (β), pattern of the catalyst and methanol-FFA molar ratio etc...
October 18, 2016: Bioresource Technology
H K Imdadul, N W M Zulkifli, H H Masjuki, M A Kalam, M Kamruzzaman, M M Rashed, H K Rashedul, Azham Alwi
Exploring new renewable energy sources as a substitute of petroleum reserves is necessary due to fulfilling the oncoming energy needs for industry and transportation systems. In this quest, a lot of research is going on to expose different kinds of new biodiesel sources. The non-edible oil from candlenut possesses the potential as a feedstock for biodiesel production. The present study aims to produce biodiesel from crude candlenut oil by using two-step transesterification process, and 10%, 20%, and 30% of biodiesel were mixed with diesel fuel as test blends for engine testing...
November 4, 2016: Environmental Science and Pollution Research International
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