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https://www.readbyqxmd.com/read/28223079/pie-waste-a-component-of-food-waste-and-a-renewable-substrate-for-producing-ethanol
#1
Margaret Magyar, Leonardo da Costa Sousa, Singaram Jayanthi, Venkatesh Balan
Sugar-rich food waste is a sustainable feedstock that can be converted into ethanol without an expensive thermochemical pretreatment that is commonly used in first and second generation processes. In this manuscript we have outlined the pie waste conversion to ethanol through a two-step process, namely, enzyme hydrolysis using commercial enzyme products mixtures and microbial fermentation using yeast. Optimized enzyme cocktail was found to be 45% alpha amylase, 45% gamma amylase, and 10% pectinase at 2.5mg enzyme protein/g glucan produced a hydrolysate with high glucose concentration...
February 17, 2017: Waste Management
https://www.readbyqxmd.com/read/28220353/exploitation-of-genus-rhodosporidium-for-microbial-lipid-production
#2
REVIEW
Jingyang Xu, Dehua Liu
Oleaginous microorganisms are receiving significant attention worldwide for their utility in biodiesel production and the potentiality to produce some specialty-type lipids. There is an increasing interest in isolation/adaption of robust microbe strains and design of innovative fermentation processes to make microbial lipid production a more efficient and economically feasible bio-process. Currently, the genus Rhodosporidium has been considered an important candidate, for the reason that several strains belonging to this genus have shown excellent capabilities of lipid accumulation, broad adaptabilities to various substrates, and co-production of some carotenoids...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28220186/efficient-gene-targeting-in-non-homologous-end-joining-deficient-lipomyces-starkeyi-strains
#3
Yoshifumi Oguro, Harutake Yamazaki, Satoshi Ara, Yosuke Shida, Wataru Ogasawara, Masamichi Takagi, Hiroaki Takaku
Microbial lipids are sustainable feedstock for the production of oleochemicals and biodiesel. Oleaginous yeasts have recently been proposed as alternative lipid producers to plants and animals to promote sustainability in the chemical and fuel industries. The oleaginous yeast Lipomyces starkeyi has great industrial potential as an excellent lipid producer. However, improvement of its lipid productivity is essential for the cost-effective production of oleochemicals and fuels. Genetic and metabolic engineering of L...
February 20, 2017: Current Genetics
https://www.readbyqxmd.com/read/28219051/simultaneous-cell-disruption-and-lipid-extraction-in-a-microalgal-biomass-using-a-nonpolar-tertiary-amine
#4
Wen-Can Huang, Jong-Duk Kim
A simultaneous cell disruption and lipid extraction method is developed for microalgal biodiesel production using a triethylamine/methanol solvent system. Individually, the pure solvents, i.e. triethylamine and methanol, do not exhibit significant enhancement in lipid extraction, but a 3:7 (v/v) triethylamine/methanol mixture exhibits the highest lipid extraction, corresponding to 150% of the conventional chloroform/methanol (2:1, v/v) solvent extraction. This extraction is equivalent to 92.5% of the total lipids, even when extracted from a wet microalgal biomass with a water content of 80%...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28218992/differently-localized-lysophosphatidic-acid-acyltransferases-crucial-for-triacylglycerol-biosynthesis-in-the-oleaginous-alga-nannochloropsis
#5
Takashi Nobusawa, Koichi Hori, Hiroshi Mori, Ken Kurokawa, Hiroyuki Ohta
Production of renewable bioenergy will be necessary to meet rising global fossil fuel demands. Members of the marine microalgae genus Nannochloropsis produce large amounts of oils (triacylglycerols; TAGs), and this genus is regarded as one of the most promising for biodiesel production. Recent genome sequencing and transcriptomic studies on Nannochloropsis have provided a foundation for understanding its oleaginous trait, but the mechanism underlying oil accumulation remains to be clarified. Here we report Nannochloropsis knockout strains of four extraplastidic lysophosphatidic acid acyltransferases (LPAT1-4), which catalyze a major de novo biosynthetic step of TAGs and membrane lipids...
February 20, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28215803/polycyclic-aromatic-hydrocarbons-pahs-produced-in-the-combustion-of-fatty-acid-alkyl-esters-from-different-feedstocks-quantification-statistical-analysis-and-mechanisms-of-formation
#6
Alberto Llamas, Ana-María Al-Lal, María-Jesús García-Martínez, Marcelo F Ortega, Juan F Llamas, Magín Lapuerta, Laureano Canoira
Polycyclic Aromatic Hydrocarbons (PAHs) are pollutants of concern due to their carcinogenic and mutagenic activity. Their emissions are mainly related with the combustion or pyrolysis of the organic matter, such as in fossil fuels combustion. It is important to characterize PAHs in the combustions of biofuels due to their increasing importance in the actual energetic setting. There is a lot of research focused in PAHs emission due to the combustion in diesel engines; but only few of them have analyzed the effect of raw material and type of alcohol used in the transesterification process...
February 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28210816/efficient-solvothermal-wet-in-situ-transesterification-of-nannochloropsis-gaditana-for-biodiesel-production
#7
Bora Kim, Yong Keun Chang, Jae W Lee
In situ transesterification of wet microalgae is a promising, simplified alternative biodiesel production process that replaces multiple operations of cell drying, extraction, and transesterification reaction. This study addresses enhanced biodiesel production from Nannochloropsis gaditana at elevated temperatures. Compared with the previously reported in situ transesterification process of conducting the reaction at a temperature ranging from 95 to 125 °C, the present work employs higher temperatures of at least 150 °C...
February 16, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28207856/biodiesel-production-potential-from-fat-fraction-of-municipal-waste-in-makkah
#8
K Shahzad, A S Nizami, M Sagir, M Rehan, S Maier, M Z Khan, O K M Ouda, I M I Ismail, A O BaFail
In the Kingdom of Saudi Arabia (KSA), millions of Muslims come to perform Pilgrimage every year. Around one million ton of municipal solid waste (MSW) is generated in Makkah city annually. The collected MSW is disposed of in the landfills without any treatment or energy recovery. As a result, greenhouse gas (GHG) emissions and contamination of the soil and water bodies along with leachate and odors are occurring in waste disposal vicinities. The composition of MSW shows that food waste is the largest waste stream (up to 51%) of the total generated MSW...
2017: PloS One
https://www.readbyqxmd.com/read/28207262/first-principles-chemical-kinetic-modeling-of-methyl-trans-3-hexenoate-epoxidation-by-ho2
#9
Stefania Cagnina, Andre Nicolle, Theodorus De Bruin, Yuri Georgievskii, Stephen J Klippenstein
The design of innovative combustion processes relies on a comprehensive understanding of biodiesel oxidation kinetics. The present study aims at unraveling the reaction mechanism involved in the epoxidation of a realistic biodiesel surrogate, methyl trans-3-hexenoate, by hydroperoxy radicals using a bottom-up theoretical kinetics methodology. The obtained rate constants are in good agreement with experimental data for alkenes epoxidation by HO2. The impact of temperature and pressure on epoxidation pathways involving H-bonded and non H-bonded conformers was assessed...
February 16, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28202317/application-of-time-of-flight-mass-spectrometry-for-screening-of-crude-glycerins-for-toxic-phorbol-ester-contaminants
#10
Kithsiri Herath, Lauren Girard, Renate Reimschuessel, Hiranthi Jayasuriya
Since 2007, the U.S. Food and Drug Administration (FDA) has received numerous complaints of pet illnesses that may be related to the consumption of jerky pet treats. Many of those treats include glycerin as an ingredient. Glycerin can be made directly from oils such as palm seed oil, but can also be derived from the seed oil of toxic Jatropha plant during biodiesel production. If crude glycerin from biodiesel production from Jatropha curcas is used in the manufacture of animal feed, toxic tigliane diterpene phorbol esters (PEs), namely Jatropha factors (JFs), may be present and could lead to animal illnesses...
January 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28197131/bacillus-spp-isolated-from-puba-as-a-source-of-biosurfactants-and-antimicrobial-lipopeptides
#11
Karla J Perez, Jaime Dos Santos Viana, Fernanda C Lopes, Jamile Q Pereira, Daniel M Dos Santos, Jamil S Oliveira, Renata V Velho, Silvia M Crispim, Jacques R Nicoli, Adriano Brandelli, Regina M D Nardi
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28196782/efficient-anaerobic-production-of-succinate-from-glycerol-in-engineered-escherichia-coli-by-using-dual-carbon-sources-and-limiting-oxygen-supply-in-preceding-aerobic-culture
#12
Qing Li, Bing Huang, Hui Wu, Zhimin Li, Qin Ye
Glycerol is an important resource for production of value-added bioproducts due to its large availability from the biodiesel industry as a by-product. In this study, two metabolic regulation strategies were applied in the aerobic stage of a two-stage fermentation to achieve high metabolic capacities of the pflB ldhA double mutant Escherichia coli strain overexpressing phosphoenolpyruvate carboxykinase (PCK) in the subsequent anaerobic stage: use of acetate as a co-carbon source of glycerol and restriction of oxygen supply in the PCK induction period...
January 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28196780/evaluating-the-effectiveness-of-various-biochars-as-porous-media-for-biodiesel-synthesis-via-pseudo-catalytic-transesterification
#13
Jechan Lee, Jong-Min Jung, Jeong-Ik Oh, Yong Sik Ok, Sang-Ryong Lee, Eilhann E Kwon
This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification. To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured. Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar. For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C...
February 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28195433/enzyme-immobilized-nanoporous-carbons-derived-from-metal-organic-framework-a-new-support-for-biodiesel-synthesis
#14
Li-Hao Liu, Yung-Han Shih, Wan-Ling Liu, Chia-Her Lin, Hsi-Ya Huang
In this study, nanoporous carbon (NPC) derived from metal-organic framework (MOF) was used as support for the immobilization of Burkholderia cepacia lipase. The decorated aluminum oxide within the mesoporous NPC facilitated the enzyme loading efficiency as well as catalytic ability for transesterification of soybean oil thus making it green and sustainable technique for industrial application.
February 13, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28190842/simultaneous-direct-determinations-of-na-mg-k-ca-p-and-s-in-biodiesel-fuel-by-icp-qms-qms-after-xylene-dilution-development-and-application-of-a-high-throughput-method-for-a-homogeneity-assessment-of-a-candidate-reference-material
#15
Yanbei Zhu, Yuko Kitamaki, Masahiko Numata
Inductively coupled plasma tandem quadrupole mass spectrometry (ICP-QMS/QMS) measurements after xylene dilution were investigated as a method for determining the elements (Na, Mg, K, Ca, P, and S) in a biodiesel fuel (BDF) candidate reference material (RM). Optimizations were respectively carried out for the following parameters to obtain the best performance for measurements: O2 flow rate (additional gas to the carrier gas) to ensure complete combustion of the xylene solvent in the plasma, plasma power to obtain lower background signal intensities for Na and K, O2 flow rate (reaction cell gas) to remove any spectral interference with the S, H2 flow rate so as to remove spectral interference with Ca...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28190806/optimization-of-the-enzymatic-synthesis-of-biodiesel-from-terminalia-cattapa-l-kernel-oil-using-response-surface-methodology
#16
Dedy Suhendra, Erin Ryantin Gunawan, Arista Dewi Nurita, Desy Komalasari, Teguh Ardianto
The synthesis of biodiesel using Terminalia cattapa L. (local language: Ketapang) kernel oil and ethanol has been conducted. The effect of variable parameters of synthesis includes reaction time (3-7 h), temperature (35-60°C), amount of enzyme (0.1-0.3 g) and substrate molar ratio (ketapang kernel oil to ethanol, 1:1-1:3) were studied. Response surface methodology based on a five-level and four-variable central composite rotatable design were used to evaluate the interactive effects of the sinthesis parameters on the percentage yield of biodiedsel...
February 10, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28190466/the-total-triterpenoid-saponins-of-xanthoceras-sorbifolia-improve-learning-and-memory-impairments-through-against-oxidative-stress-and-synaptic-damage
#17
Xue-Fei Ji, Tian-Yan Chi, Peng Liu, Lu-Yi Li, Ji-Kai Xu, Qian Xu, Li-Bo Zou, Da-Li Meng
BACKGROUND: X. sorbifolia is a widely cultivated ecologicalcrop in the north of China which is used to produce biodiesel fuel. It also possesses special medicinal value and has attracted keen interests of researchers to explore its bioactivity. PURPOSE: To extract the total triterpenoid saponins from the husk of X. sorbifolia (TSX) and investigate its effects on Alzheimer's disease (AD). STUDY DESIGN: TSX was prepared via modern extraction techniques...
February 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28188644/rnai-based-targeted-gene-knockdown-in-the-model-oleaginous-microalgae-nannochloropsis-oceanica
#18
Li Wei, Yi Xin, Qintao Wang, Juan Yang, Hanhua Hu, Jian Xu
Microalgae are promising feedstock for renewable fuels such as biodiesel, yet development of industrial oleaginous strains has been hindered by the paucity and inefficiency of reverse genetics tools. Here we established an efficient RNAi-based targeted gene-knockdown method for Nannochloropsis spp., which are emerging model organisms for industrial microalgal oil production. The method achieved a 40-80% success rate in Nannochloropsis oceanica strain IMET1. When transcript level of one carbonic anhydrase (CA) was inhibited by 62-83% via RNAi, mutant cells exhibited photosynthetic oxygen evolution (POE) rates that were 68-100% higher than wild-type (WT) at pH 6...
November 7, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28187768/current-lipid-extraction-methods-are-significantly-enhanced-adding-a-water-treatment-step-in-chlorella-protothecoides
#19
Xiaojie Ren, Xinhe Zhao, François Turcotte, Jean-Sébastien Deschênes, Réjean Tremblay, Mario Jolicoeur
BACKGROUND: Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show efficiency limitation and until now, extraction protocols have not been fully optimized for specific lipid compounds. The present study thus presents a novel lipid extraction method, consisting in the addition of a water treatment of biomass between the two-stage solvent extraction steps of current extraction methods...
February 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28169024/a-sustainable-use-of-ricotta-cheese-whey-for-microbial-biodiesel-production
#20
Eleonora Carota, Silvia Crognale, Alessandro D'Annibale, Anna Maria Gallo, Silvia Rita Stazi, Maurizio Petruccioli
The increasing demand of plant oils for biodiesel production has highlighted the need for alternative strategies based either on non-food crops or agro-industrial wastes that do not compete with food and feed production. In this context, the combined use of wastewater and oleaginous microorganisms could be a valuable production option. Ricotta cheese whey (RCW), one of the major byproducts of the dairy industry, is produced in very high and steadily increasing amounts and, due to its high organic load, its disposal is cost-prohibitive...
February 3, 2017: Science of the Total Environment
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