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C C Wang, J Y Tan, Y Q Ma, L H Liu
The optical constants of vegetable oils and biodiesels are the basic input parameters in the study of the thermal radiation transfer and monitoring the productivity of vegetable oils converting to biodiesels. In this work, a combined ellipsometry-transmission method is presented to obtain the optical constants of palm oil and palm oil biodiesel between 20°C and 150°C in the spectral range 600-4100  cm(-1) and to study the temperature effect on the optical constants. In the combined method, a modified ellipsometry method is used to measure the optical constants of palm oil and palm oil biodiesel for the whole researched wave bands...
June 20, 2017: Applied Optics
Muhammad Qasim, Tariq Mahmood Ansari, Mazhar Hussain
The waste tyre and waste cooking oils have a great potential to be used as alternative fuels for diesel engines. The aim of this study was to convert light fractions of pyrolysis oil derived from Pakistani waste vehicle tyres and waste soybean oil methyl esters into valuable fuel and to reduce waste disposal-associated environmental problems. In this study, the waste tyre pyrolysis liquid (light fraction) was collected from commercial tyre pyrolysis plant and biodiesel was prepared from waste soybean oil. The fuel blends (FMWO10, FMWO20, FMWO30, FMWO40 and FMWO50) were prepared from a 30:70 mixture of waste tyre pyrolysis liquid and waste soybean oil methyl esters with different proportions of mineral diesel...
October 18, 2017: Environmental Science and Pollution Research International
Feng Li, Changji Yin, Liming Sun, Yuanxiu Li, Xuewu Guo, Hao Song
Microbial fuel cell (MFC) is an eco-friendly bio-electrochemical system that uses mi-croorganism as biocatalyst to convert biomass into electricity. Glycerol, as a waste in the biodiesel refinery processes, is an appealing substrate for MFC. Nevertheless, glycerol cannot be utilized as carbon source by well-known exoelectrogens such as Shewanella oneidensis. Herein, to generate electricity by rapidly harnessing glycerol, we rationally constructed a Klebsiella pneumoniae-Shewanella oneidensis microbial consortium to efficiently harvest electricity from glycerol, in which K...
October 18, 2017: Biotechnology Journal
Xue Li, Edmund Mupondwa, Lope Tabil
This study undertakes technoeconomic analysis of commercial production of hydro-processed renewable jet (HRJ) fuel from camelina oil in the Canadian Prairies. An engineering economic model designed in SuperPro Designer® investigated capital investment, scale, and profitability of producing HRJ and co-products (biodiesel, naphtha, LPG, and propane) based on biorefinery plant sizes of 112.5-675 million L annum(-1). Under base case scenario, the minimum selling price (MSP) of HRJ was $1.06 L(-1) for a biorefinery plant with size of 225 million L...
September 28, 2017: Bioresource Technology
Farha Deeba, Alok Patel, Neha Arora, Vikas Pruthi, Parul A Pruthi, Yuvraj S Negi
The potential of lipid accumulation by oleaginous yeast Cryptococcus vishniaccii grown on amaranth seed aqueous extract (AAE) media was assessed. Maximum cell biomass productivity of 104 mg/L/h, lipid productivity of 54 mg/L/h, and lipid content of 52.31% were recorded on AAE when carbon to nitrogen (C:N) ratio increased from 134 to 147 after removal of ammonia nitrogen. The lipid droplet (LD) size (2.32 ± 0.38 μm) was visualized by fluorescence microscopy using Nile red stain indicating maximum accumulated triacylglycerol (TAG) at C:N 147...
October 16, 2017: Environmental Science and Pollution Research International
Abd El-Fatah Abomohra, Amal Hamed El-Naggar, Areej Ali Baeshen
Nowadays, biofuel production is a fast expanding industry and is facing a growing dilemma about a feedstock source capable of keeping up with demand. Recently, macroalgae have been attracting a wide attention as a source for biofuel. In the present study, ten macroalgae were collected and screened as biodiesel feedstocks. As a result of their high biomass production and relatively high lipid content, Ulva lactuca, Padina boryana and Ulva intestinalis showed the highest significant lipids and fatty acid methyl esters (FAMEs) areal productivities among the studied species...
October 13, 2017: Journal of Bioscience and Bioengineering
Thanongsak Chaiyaso, Atchara Manowattana
Bioconversion of biodiesel-derived crude glycerol into carotenoids and lipids was investigated by a microbial conversion of an oleaginous red yeast Sporidiobolus pararoseus KM281507. The methanol content in crude glycerol (0.5% w/v) did not show a significant effect on biomass production by strain KM281507. However, demethanolized crude glycerol significantly supported the production of biomass (8.64 ± 0.13 g/L), lipids (2.92 ± 0.03 g/L), β-carotene (15.76 ± 0.85 mg/L) and total carotenoids (33...
October 16, 2017: Preparative Biochemistry & Biotechnology
Zhangcai Luo, Jing Miao, Wei Luo, Guoying Li, Yao Du, Xiaobin Yu
OBJECTIVE: To explore an efficient use of crude glycerol for the production of a highly thermostable β-mannanase (ReTMan26) by Pichia pastoris X33. RESULTS: Cell growth was significantly inhibited by 4 and 6% (w/v) crude glycerol in 250 ml shake-flasks and in 5 l bioreactor batch cultures, respectively, but not affected by pure glycerol at the same concentrations. For further study, the impact of various impurities in crude glycerol on the cell growth of, and ReTMan26 production by, Pichia pastoris was investigated...
October 12, 2017: Biotechnology Letters
Laimis Bauša, Dainius Steponavičius, Eglė Jotautienė, Aurelija Kemzūraitė, Ernestas Zaleckas
BACKGROUND: Rape (Brassica napus L.) is a major global oilseed crop characterised by its high potential as an alimentary oil and in biodiesel production. The two most popular pod sealants (PS) used to reduce rape pod shattering are products in the pinolene range (di-1-p-menthene) and latex polymer products. Reports on the effective preservation of seed yield by these products are fairly contradictory. With this in mind, an experimental PS (PS4) that contained the active agents acrylic and trisiloxane was developed...
October 11, 2017: Journal of the Science of Food and Agriculture
César A Godoy
Immobilization on Glyoxyl-agarose support (Gx) is one of the best strategies to stabilize enzymes. However, the strategy is difficult to apply at neutral pH when most enzymes are stable and, even when possible, produces labile derivatives. This work contributes to overcoming this hurdle through a strategy that combines solid-phase amination, presence of key additives, and derivative basification. To this end, aminated industrial lipases from Candida artarctica (CAL), Thermomyces lunuginosus (TLL), and the recombinant Geobacillus thermocatenulatus (BTL2) were immobilized on Gx for the first time at neutral pH using anthranilic acid (AA) or DTT as additives (immobilization yields >70%; recovered activities 37...
October 12, 2017: International Journal of Molecular Sciences
Jiajia Zhang, Fang Cui, Linxu Xu, Xinzi Pan, Xiaoqiang Wang, Xiao Zhang, Tieyu Cui
Herein, a novel and facile high-yield strategy is reported to efficiently fabricate 1D self-supported Au/CaO nanocatalysts using dual metal co-ordination polymers as templates. Significantly, a uniform distribution of dual metal nanoparticles can be ensured due to the fact that both Ca(2+) and Au(3+) ions are initially introduced into the co-ordination polymer via chemical bonding with bifunctional organic linkers and then, the Au and CaO nanoparticles are formed simultaneously in one pot via calcination. Furthermore, the as-prepared Au/CaO nanoribbons exhibit excellent catalytic performance in the transesterification reaction, which can be attributed to the small size and high distribution of CaO nanoparticles as well as the special 1D structure with high surface area...
October 12, 2017: Nanoscale
Hongwu Cui, Fanping Meng, Feng Li, Yuejie Wang, Weiyan Duan, Yichen Lin
This study proposes a two-stage mixotrophic process for cultivating Chlorella vulgaris. Heterotrophic growth is the dominant step in Phase I (to increase microalgal biomass) and photoautotrophic growth occurs in Phase II (to improve biomass concentration and lipid production). The results show that the addition of the low-cost antioxidant sodium erythorbate (8 g L(-1)) significantly accelerates the growth of microalgae in the first stage with air aeration. Furthermore, a higher CO2 fixation rate was obtained in the second stage (at least 344...
October 10, 2017: AMB Express
Nacer Bellaloui, James R Smith, Alemu Mengistu
The timing of harvest is a major factor affecting seed quality in soybean, particularly in Midsouthern USA, when rain during harvest period is not uncommon. The objective of this research was to evaluate the effects of time of harvest on soybean seed quality (seed composition, germination, seed coat boron, and lignin) in high germinability (HG) breeding lines (50% exotic) developed under high heat. The hypothesis was that seeds of HG lines possess physiological and genetic traits for a better seed quality at harvest maturity and delayed harvest...
2017: Frontiers in Plant Science
Mauricio Ramírez-Castrillón, Victoria P Jaramillo-Garcia, Priscila D Rosa, Melissa F Landell, Duong Vu, Mariana F Fabricio, Marco A Z Ayub, Vincent Robert, João A P Henriques, Patricia Valente
A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast, Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38% for glucose or 34.97% for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a biodiesel refinery...
2017: Frontiers in Microbiology
Mengmeng Zhu, Sarah M Assmann
Drought can severely damage crops, resulting in major yield losses. During drought, vascular land plants conserve water via stomatal closure. Each stomate is bordered by a pair of guard cells that shrink in response to drought and the associated hormone abscisic acid (ABA). The activation of complex intracellular signaling networks underlies these responses. Therefore, analysis of guard cell metabolites is fundamental for elucidation of guard cell signaling pathways. Brassica napus is an important oilseed crop for human consumption and biodiesel production...
October 9, 2017: Scientific Reports
Santiago Rodriguez-Perez, Antonio Serrano, Alba A Pantión, Bernabé Alonso-Fariñas
The search for new materials that replace fossil fuel-based plastics has been focused on biopolymers with similar physicochemical properties to fossil fuel-based plastics, such as Polyhydroxyalkanoates (PHA). The present paper reviews the challenges of scaling-up PHA production from waste streams during the period from 2014 to 2016, focusing on the feasibility of the alternatives and the most promising alternatives to its scaling-up. The reviewed research studies mainly focus on reducing costs or obtaining more valuable polymers...
October 5, 2017: Journal of Environmental Management
Guo-Hua Dao, Guang-Xue Wu, Xiao-Xiong Wang, Lin-Lan Zhuang, Tian-Yuan Zhang, Hong-Ying Hu
Microalgae are potential candidates for the production of valuable products, such as renewable biodiesel, health products and pigments. However, low biomass productivity has restricted their large-scale applications. In this study, the effects of two auxins (one natural type of indole-3-acetic acid (IAA) and the other synthetic type of 2,4-dichlorophenoxyacetic acid (2,4-D)) on the growth and fatty acid methyl esters (FAMEs) production of a freshwater microalgae Scenedesmus sp. LX1 were investigated. Both auxins showed a "low dosage-promotion and high dosage-inhibition" effect on the growth and FAMEs accumulation...
September 14, 2017: Bioresource Technology
Parveen Kumar Sharma, Rifat Munir, Teresa de Kievit, David B Levin
Pseudomonas chlororaphis strain PA23 was isolated from soybean roots as a plant growth-promoting rhizobacterium (PGPR) and secretes a wide-range of compounds, including the antibiotics phenazine-1-caroxymide (PCA), pyrrolnitrin, and 2-hydroxyphenazine. We have determined that P. chlororaphis PA23 can synthesize medium chain length polyhydroxyalkanoate (PHA) polymers utilizing free fatty acids, such as octanoic acid and nonanoic acid, as well as vegetable oils as sole carbon sources. Genome analysis identified a pha operon containing 7 genes in P...
October 5, 2017: Canadian Journal of Microbiology
Sudesh Chhikara, Hesham M Abdullah, Parisa Akbari, Danny Schnell, Om Parkash Dhankher
Plant seed oils based liquid transportation fuels (i.e., biodiesel and green diesel) have tremendous potential as environmentally, economically and technologically feasible alternatives to petroleum-derived fuels. Due to their nutritional and industrial importance, one of the major objectives is to increase the seed yield and oil production of oilseed crops via biotechnological approaches. Camelina sativa, an emerging oilseed crop, has been proposed as an ideal crop for biodiesel and bioproducts applications...
October 4, 2017: Plant Biotechnology Journal
K S Simões, S A Silva, E L Machado, M S Silva
Castor bean (Ricinus communis L.) is a tropical plant of great commercial interest and a potential source of biodiesel. The development of genetically improved cultivars with high amounts of oil in the seeds and low ricin toxicity is crucial to increase the productivity of this crop. The use of TRAP (target region amplification polymorphism) markers to develop elite lineages and study genetic divergence is fundamental to advance the genetic improvement of this species. The goal of this study was to evaluate the genetic divergence among 40 elite lineages of R...
September 27, 2017: Genetics and Molecular Research: GMR
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