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RII beta

Naicheng Wu, Yueming Qu, Björn Guse, Kristė Makarevičiūtė, Szewing To, Tenna Riis, Nicola Fohrer
There has been increasing interest in algae-based bioassessment, particularly, trait-based approaches are increasingly suggested. However, the main drivers, especially the contribution of hydrological variables, of species composition, trait composition, and beta diversity of algae communities are less studied. To link species and trait composition to multiple factors (i.e., hydrological variables, local environmental variables, and spatial factors) that potentially control species occurrence/abundance and to determine their relative roles in shaping species composition, trait composition, and beta diversities of pelagic algae communities, samples were collected from a German lowland catchment, where a well-proven ecohydrological modeling enabled to predict long-term discharges at each sampling site...
March 2018: Ecology and Evolution
Kosha J Mehta, Jason D Coombes, Marco Briones-Orta, Paul P Manka, Roger Williams, Vinood B Patel, Wing-Kin Syn
BACKGROUND: Although excess iron induces oxidative stress in the liver, it is unclear whether it directly activates the hepatic stellate cells (HSC). MATERIALS AND METHODS: We evaluated the effects of excess iron on fibrogenesis and transforming growth factor beta (TGF-β) signaling in murine HSC. Cells were treated with holotransferrin (0.005-5g/L) for 24 hours, with or without the iron chelator deferoxamine (10µM). Gene expressions (α-SMA, Col1-α1, Serpine-1, TGF-β, Hif1-α, Tfrc and Slc40a1) were analyzed by quantitative real time-polymerase chain reaction, whereas TfR1, ferroportin, ferritin, vimentin, collagen, TGF-β RII and phospho-Smad2 proteins were evaluated by immunofluorescence, Western blot and enzyme-linked immunosorbent assay...
February 2018: American Journal of the Medical Sciences
Miri Assayag, Shabtai Moshel, Martin Kohan, Neville Berkman
BACKGROUND: Omalizumab is an anti-immunoglobulin E (IgE) monoclonal antibody used in the treatment of severe asthma. Its therapeutic efficacy is primarily attributed to reduction of serum-free IgE and in the expression of high-affinity IgE receptor, fc epsilon RI. However, its effect on the low-affinity IgE receptor fc epsilon RII/CD23 in vivo has not been evaluated. AIM: To determine whether CD23 plays a role in the inflammatory process in severe uncontrolled asthma and whether anti-IgE therapy modulates fc epsilon RII/CD23 expression in these patients...
January 1, 2018: Allergy and Asthma Proceedings:
Eduardo K Moioli, Clifford Hsieh, Angela Tisch, Diana Bolotin
BACKGROUND: The histologic status of squamous cell carcinoma in situ (SCC-IS) after diagnostic biopsy has not been well described or compared between immunocompetent and immunosuppressed patients. Expression of immunohistochemical (IHC) markers of aggressive SCC has not been compared between SCC-IS that clears or becomes invasive after biopsy. OBJECTIVE: To determine the histologic status of SCC-IS after diagnostic biopsy in these populations. METHODS: Retrospective analysis of 129 patients with SCC-IS treated with excision and 55 patients treated with Mohs surgery...
October 19, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Laerke Smedegaard, Mette Gitz Charlot, Gunnar Hilmar Gislason, Peter Riis Hansen
AIM: The present study aimed to investigate temporal trends in myocardial infarction (MI) presentation with or without ST-segment elevation (STE) and the association with use of cardioprotective drugs prior to admission. METHODS AND RESULTS: Using individual-level linkage of data from Danish nationwide registries, we identified all patients 30 years or older admitted with a first-time MI in the period 2003-2012, and their use of cardioprotective drugs 6 months prior to admission...
May 16, 2017: European Heart Journal. Cardiovascular Pharmacotherapy
Ana P B Araujo, Luan P Diniz, Cristiane M Eller, Beatriz G de Matos, Rodrigo Martinez, Flávia C A Gomes
Granule cells (GC) are the most numerous glutamatergic neurons in the cerebellar cortex and represent almost half of the neurons of the central nervous system. Despite recent advances, the mechanisms of how the glutamatergic synapses are formed in the cerebellum remain unclear. Among the TGF-β family, TGF-beta 1 (TGF-β1) has been described as a synaptogenic molecule in invertebrates and in the vertebrate peripheral nervous system. A recent paper from our group demonstrated that TGF-β1 increases the excitatory synapse formation in cortical neurons...
2016: Frontiers in Cellular Neuroscience
Claudia Magalhães Calvet, Tatiana Araújo Silva, Tatiana Galvão DE Melo, Tânia Cremonini DE Araújo-Jorge, Mirian Claudia DE Souza Pereira
Transforming growth factor beta (TGF-β) cytokine is involved in Chagas disease establishment and progression. Since Trypanosoma cruzi can modulate host cell receptors, we analysed the TGF-β receptor type II (TβRII) expression and distribution during T. cruzi - cardiomyocyte interaction. TβRII immunofluorescent staining revealed a striated organization in cardiomyocytes, which was co-localized with vinculin costameres and enhanced (38%) after TGF-β treatment. Cytochalasin D induced a decrease of 45·3% in the ratio of cardiomyocytes presenting TβRII striations, demonstrating an association of TβRII with the cytoskeleton...
May 2016: Parasitology
Xiaohua Wang, Haifeng Liu, Yawei Liu, Yuting Li, Lei Yan, Xiaohuan Yuan, Yufei Zhang, Yan Wu, Jieting Liu, Chunlei Zhang, Yanhui Chu
Ubiquitin-like protease 1 (Ulp1) of Saccharomyces cerevisiae emerges as a fundamental tool to obtain the natural N-terminal target protein by cleavage of the small ubiquitin-related modifier (SUMO) fusion protein. However, the costly commercial Ulp1 and its complicated procedures limit its application in the preparation of the target protein with natural N-terminal sequence. Here, we describe the preparation of bioactive codon-optimized recombinant truncated Ulp1 (Leu403-Lys621) (rtUlp1) of S. cerevisiae in Escherichia coli using only one-step with Ni-NTA affinity chromatograph, and the application of rtUlp1 to cleave the SUMO fusion protein by simply mixing the purified rtUlp1, SUMO fusion protein and DL-Dithiothreitol in Tris-HCl buffer...
April 2016: Protein Journal
Xue Wang, Zhi Yang, Ying Xiong, Ping Wan, Li He, Yuan Chen, Song Jiang, Hong Su, Yi-Qiong Zhang, Yan-Fei Du
OBJECTIVE: The purpose of this study was to investigate the effects of different fluences of Q-switched 1064 nm Nd:YAG laser on skin repair and barrier, and clarify its mechanisms. BACKGROUND DATA: The Q-switched 1064 nm Nd:YAG laser is widely used for rejuvenation, which needs appropriate fluence data to optimize efficacy and minimize side effects, and for elucidation of action mechanism. MATERIALS AND METHODS: The dorsal skin of BABL/c mice was administered 0, 1, 1...
February 2016: Photomedicine and Laser Surgery
Gabriel Ruiz-Castañeda, Norma Dominguez-Avila, Javier González-Ramírez, Nora Fernandez-Jaramillo, Jorge Escoto-Herrera, Fausto Sánchez-Muñoz, Luis Manuel Amezcua-Guerra, Ricardo Marquez-Velasco, Rafael Bojalil, Roman Espinosa-Cervantes, Fausto Sánchez
We determined mRNA expression of genes of endothelin-1 (ET-1), and of the transforming growth factor beta ligands (TGFβ1, TGFβ2 and TGFβ3), their receptors (TβRI and TβRII) and their pseudoreceptor BAMBI in the heart of broilers raised under cold temperature conditions and affected by pulmonary hypertension. Gene expression was determined by RT-qPCR in right myocardial ventricle samples from 4-week-old chickens (n = 48) raised either under normal (control) or cold temperature conditions (22 °C versus 14 °C)...
November 13, 2015: Journal of Receptor and Signal Transduction Research
Liangping Zhang, Yang Wang, Rui Lei, Hui Shen, Yichen Shen, Zhinan Wu, Jinghong Xu
OBJECTIVE: To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-β receptor II (TβRII) in dermal fibroblasts. METHODS: A lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells...
August 2015: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Xiaoyan Zhu, Duo Xu, Xiaomin Zhu, Lei Li, Haijun Li, Feng Guo, Xia Chen, Yan Tan, Lin Xie
PURPOSE: This study was designed to develop a chitosan (CS) thermo-sensitive gel combined with aptamer S58 targeting transforming growth factor-beta receptor II (TGF-β RII) and to investigate the antifibrotic effects of CS/S58 gel in a rat glaucoma filtration surgery (GFS) model. METHODS: In vitro aptamer S58 release rate from the CS/S58 gel were detected, and the effect of mitomycin-C (MMC), TGF-β2, CS, or CS/S58 gel on wound healing were investigated in a rat GFS model by detecting scar-related factors and the involved inflammatory response...
August 2015: Investigative Ophthalmology & Visual Science
X Meng, A Vander Ark, P Lee, G Hostetter, N A Bhowmick, L M Matrisian, B O Williams, C K Miranti, X Li
Breast cancer (BCa) bone metastases cause osteolytic bone lesions, which result from the interactions of metastatic BCa cells with osteoclasts and osteoblasts. Osteoclasts differentiate from myeloid lineage cells. To understand the cell-specific role of transforming growth factor beta (TGF-β) in the myeloid lineage, in BCa bone metastases, MDA-MB-231 BCa cells were intra-tibially or intra-cardially injected into LysM(Cre)/Tgfbr2(floxE2/floxE2) knockout (LysM(Cre)/Tgfbr2 KO) or Tgfbr2(floxE2/floxE2) mice. Metastatic bone lesion development was compared by analysis of both lesion number and area...
May 5, 2016: Oncogene
Yuxiu Gao, Ning Shan, Cheng Zhao, Yunhai Wang, Fuliang Xu, Jiacun Li, Xiaoqian Yu, Lifeng Gao, Zhengjun Yi
BACKGROUND AND OBJECTIVE: Ovarian cancer is among the most lethal of all malignancies in women. While chemotherapy is the preferred treatment modality, chemoresistance severely limits treatment success. Because transforming growth factor-beta (TGF-β) could increase survival of ovarian cancer cells in the presence of cisplatin, we conducted a preclinical study of the antitumor effects of the TGF-β type I (TβRI) and type II (TβRII) kinase inhibitor LY2109761 in combination with cisplatin...
2015: International Journal of Clinical and Experimental Pathology
Zeinab Shirvani-Farsani, Mehrdad Behmanesh, Seyed Mahdi Mohammadi, Abdorreza Naser Moghadasi
AIM: A variety of evidence suggests that vitamin D can prevent the development of multiple sclerosis (MS). TGF-β pathway genes also play important roles in MS. Here, we aim to study whether vitamin D affects TGF-β pathway gene expression and Expanded Disability Status Scale (EDSS) scores in MS patients. MAIN METHODS: A randomized clinical trial was conducted on 31 relapsing-remitting (RR) MS patients. Using real-time RT-PCR, we tested the levels of TGF-β2, TGF-βRI and TGF-βRII mRNAs in the RRMS patients before and after 8 weeks of supplementation with vitamin D...
August 1, 2015: Life Sciences
Sheng Liu, Thao Nheu, Rodney Luwor, Sandra E Nicholson, Hong-Jian Zhu
Appropriate cellular signaling is essential to control cell proliferation, differentiation, and cell death. Aberrant signaling can have devastating consequences and lead to disease states, including cancer. The transforming growth factor-β (TGF-β) signaling pathway is a prominent signaling pathway that has been tightly regulated in normal cells, whereas its deregulation strongly correlates with the progression of human cancers. The regulation of the TGF-β signaling pathway involves a variety of physiological regulators...
July 17, 2015: Journal of Biological Chemistry
You-You Xia, Li Yin, Ning Jiang, Wen-Jie Guo, Hao Tian, Xue-Song Jiang, Jing Wu, Meng Chen, Jian-Zhong Wu, Xia He
BACKGROUND: Epithelial-mesenchymal transition (EMT) is associated with invasion and metastasis of cancer cells. High-mobility group AT-hook 2 (HMGA2) has been found to play a critical role in EMT in a number of malignant tumors. However, whether HMGA2 regulates the EMT in human nasopharyngeal carcinoma (NPC) is unclear. OBJECTIVE: The aim of this study was to investigate the effect and mechanism of HMGA2 in inducing invasion and migration in NPC. METHODS: In NPC tissues samples, the association of HMGA2 mRNA expression with clinicopathological characteristics were estimated by real-time quantitative RT-PCR(qRT-PCR)...
July 31, 2015: Biochemical and Biophysical Research Communications
Sang Chul Lee, Hye Jin Jeong, Byung-Jo Choi, Say-June Kim
BACKGROUND: Optimizing the hepatic regenerative capacity is an immediate priority after partial hepatectomy (PH). Recent reports have indicated improvement in liver regeneration after splenectomy, raising interest on the role of the spleen in liver regeneration. However, little is known about the exact mechanism underlying these effects. MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats randomly underwent either 70% PH only (PH, n = 25) or 70% PH combined with splenectomy (PHS, n = 25)...
June 15, 2015: Journal of Surgical Research
Barbara Halper, Marlene Hofmann, Stefan Oesen, Bernhard Franzke, Petra Stuparits, Claudia Vidotto, Harald Tschan, Norbert Bachl, Eva-Maria Strasser, Michael Quittan, Karl-Heinz Wagner, Barbara Wessner
The TGF-β superfamily has been shown to play an important role in a wide range of physiological as well as pathological processes including ageing, immune modulation, atherosclerosis and cancer development. The aim of the current study was to investigate (i) whether TGF-β signalling in peripheral blood mononuclear cells (PBMCs) would differ between young and old females and (ii) whether physical performance parameters of elderly women would be related to the expression of TGF-β or its receptors. Sixteen healthy young (22-28 years; YF) and 90 healthy older (65-92 years; OF) females participated in the study...
2015: Exercise Immunology Review
Petra Balogh, Márton Magyar, Arnold Szabó, Nándor Müllner, István Likó, Attila Patócs, Anna L Kiss
We previously showed that intraperitoneal administration of Freund's adjuvant treatment resulted in acute peritonitis and TGF-β was found to be one of the main organizers of the subsequent EMT in mesothelial cells. In the present study, we investigated whether TGF-β signaling molecules are present in mesothelial cells and how their compartmentalization pattern changes with the dynamics of inflammatory events in vivo. In addition, we tried to evaluate the turnover of endosomal compartments concomitant with the internalization of signaling molecules and examine whether caveola-mediated internalization might play a role in the termination of TGF-β signaling...
May 2015: European Journal of Cell Biology
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