keyword
MENU ▼
Read by QxMD icon Read
search

RII beta

keyword
https://www.readbyqxmd.com/read/27199658/effects-of-transforming-growth-factor-beta-1-in-cerebellar-development-role-in-synapse-formation
#1
Ana P B Araujo, Luan P Diniz, Cristiane M Eller, Beatriz G de Matos, Rodrigo Martinez, Flávia C A Gomes
Granule cells (GC) are the most numerous glutamatergic neurons in the cerebellar cortex and represent almost half of the neurons of the central nervous system. Despite recent advances, the mechanisms of how the glutamatergic synapses are formed in the cerebellum remain unclear. Among the TGF-β family, TGF-beta 1 (TGF-β1) has been described as a synaptogenic molecule in invertebrates and in the vertebrate peripheral nervous system. A recent paper from our group demonstrated that TGF-β1 increases the excitatory synapse formation in cortical neurons...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/26996782/tgf-%C3%AE-receptor-type-ii-costameric-localization-in-cardiomyocytes-and-host-cell-tgf-%C3%AE-response-is-disrupted-by-trypanosoma-cruzi-infection
#2
Claudia Magalhães Calvet, Tatiana Araújo Silva, Tatiana Galvão DE Melo, Tânia Cremonini DE Araújo-Jorge, Mirian Claudia DE Souza Pereira
Transforming growth factor beta (TGF-β) cytokine is involved in Chagas disease establishment and progression. Since Trypanosoma cruzi can modulate host cell receptors, we analysed the TGF-β receptor type II (TβRII) expression and distribution during T. cruzi - cardiomyocyte interaction. TβRII immunofluorescent staining revealed a striated organization in cardiomyocytes, which was co-localized with vinculin costameres and enhanced (38%) after TGF-β treatment. Cytochalasin D induced a decrease of 45·3% in the ratio of cardiomyocytes presenting TβRII striations, demonstrating an association of TβRII with the cytoskeleton...
May 2016: Parasitology
https://www.readbyqxmd.com/read/26960810/a-novel-strategy-for-the-preparation-of-codon-optimized-truncated-ulp1-and-its-simplified-application-to-cleavage-the-sumo-fusion-protein
#3
Xiaohua Wang, Haifeng Liu, Yawei Liu, Yuting Li, Lei Yan, Xiaohuan Yuan, Yufei Zhang, Yan Wu, Jieting Liu, Chunlei Zhang, Yanhui Chu
Ubiquitin-like protease 1 (Ulp1) of Saccharomyces cerevisiae emerges as a fundamental tool to obtain the natural N-terminal target protein by cleavage of the small ubiquitin-related modifier (SUMO) fusion protein. However, the costly commercial Ulp1 and its complicated procedures limit its application in the preparation of the target protein with natural N-terminal sequence. Here, we describe the preparation of bioactive codon-optimized recombinant truncated Ulp1 (Leu403-Lys621) (rtUlp1) of S. cerevisiae in Escherichia coli using only one-step with Ni-NTA affinity chromatograph, and the application of rtUlp1 to cleave the SUMO fusion protein by simply mixing the purified rtUlp1, SUMO fusion protein and DL-Dithiothreitol in Tris-HCl buffer...
April 2016: Protein Journal
https://www.readbyqxmd.com/read/26840551/the-effects-of-different-fluences-of-1064%C3%A2-nm-q-switched-nd-yag-laser-on-skin-repair-and-skin-barrier-dysfunction-in-mice
#4
Xue Wang, Zhi Yang, Ying Xiong, Ping Wan, Li He, Yuan Chen, Song Jiang, Hong Su, Yi-Qiong Zhang, Yan-Fei Du
OBJECTIVE: The purpose of this study was to investigate the effects of different fluences of Q-switched 1064 nm Nd:YAG laser on skin repair and barrier, and clarify its mechanisms. BACKGROUND DATA: The Q-switched 1064 nm Nd:YAG laser is widely used for rejuvenation, which needs appropriate fluence data to optimize efficacy and minimize side effects, and for elucidation of action mechanism. MATERIALS AND METHODS: The dorsal skin of BABL/c mice was administered 0, 1, 1...
February 2016: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/26565609/myocardial-expression-of-transforming-growth-factor-beta-family-and-endothelin-1-in-the-progression-from-heart-failure-to-ascites-in-broilers-with-cold-induced-pulmonary-hypertension
#5
Gabriel Ruiz-Castañeda, Norma Dominguez-Avila, Javier González-Ramírez, Nora Fernandez-Jaramillo, Jorge Escoto-Herrera, Fausto Sánchez-Muñoz, Luis Manuel Amezcua-Guerra, Ricardo Marquez-Velasco, Rafael Bojalil, Roman Espinosa-Cervantes, Fausto Sánchez
We determined mRNA expression of genes of endothelin-1 (ET-1), and of the transforming growth factor beta ligands (TGFβ1, TGFβ2 and TGFβ3), their receptors (TβRI and TβRII) and their pseudoreceptor BAMBI in the heart of broilers raised under cold temperature conditions and affected by pulmonary hypertension. Gene expression was determined by RT-qPCR in right myocardial ventricle samples from 4-week-old chickens (n = 48) raised either under normal (control) or cold temperature conditions (22 °C versus 14 °C)...
November 13, 2015: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/26403732/-nuclear-factor-i-c-inhibits-platelet-derived-growth-factor-induced-enhancement-of-dermal-fibroblast-sensitivity-to-tgf-%C3%AE
#6
Liangping Zhang, Yang Wang, Rui Lei, Hui Shen, Yichen Shen, Zhinan Wu, Jinghong Xu
OBJECTIVE: To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-β receptor II (TβRII) in dermal fibroblasts. METHODS: A lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells...
August 2015: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/26284552/evaluation-of-chitosan-aptamer-targeting-tgf-%C3%AE-receptor-ii-thermo-sensitive-gel-for-scarring-in-rat-glaucoma-filtration-surgery
#7
Xiaoyan Zhu, Duo Xu, Xiaomin Zhu, Lei Li, Haijun Li, Feng Guo, Xia Chen, Yan Tan, Lin Xie
PURPOSE: This study was designed to develop a chitosan (CS) thermo-sensitive gel combined with aptamer S58 targeting transforming growth factor-beta receptor II (TGF-β RII) and to investigate the antifibrotic effects of CS/S58 gel in a rat glaucoma filtration surgery (GFS) model. METHODS: In vitro aptamer S58 release rate from the CS/S58 gel were detected, and the effect of mitomycin-C (MMC), TGF-β2, CS, or CS/S58 gel on wound healing were investigated in a rat GFS model by detecting scar-related factors and the involved inflammatory response...
August 2015: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26279296/myeloid-specific-tgf-%C3%AE-signaling-in-bone-promotes-basic-fgf-and-breast-cancer-bone-metastasis
#8
X Meng, A Vander Ark, P Lee, G Hostetter, N A Bhowmick, L M Matrisian, B O Williams, C K Miranti, X Li
Breast cancer (BCa) bone metastases cause osteolytic bone lesions, which result from the interactions of metastatic BCa cells with osteoclasts and osteoblasts. Osteoclasts differentiate from myeloid lineage cells. To understand the cell-specific role of transforming growth factor beta (TGF-β) in the myeloid lineage, in BCa bone metastases, MDA-MB-231 BCa cells were intra-tibially or intra-cardially injected into LysM(Cre)/Tgfbr2(floxE2/floxE2) knockout (LysM(Cre)/Tgfbr2 KO) or Tgfbr2(floxE2/floxE2) mice. Metastatic bone lesion development was compared by analysis of both lesion number and area...
May 5, 2016: Oncogene
https://www.readbyqxmd.com/read/26191185/ly2109761-enhances-cisplatin-antitumor-activity-in-ovarian-cancer-cells
#9
Yuxiu Gao, Ning Shan, Cheng Zhao, Yunhai Wang, Fuliang Xu, Jiacun Li, Xiaoqian Yu, Lifeng Gao, Zhengjun Yi
BACKGROUND AND OBJECTIVE: Ovarian cancer is among the most lethal of all malignancies in women. While chemotherapy is the preferred treatment modality, chemoresistance severely limits treatment success. Because transforming growth factor-beta (TGF-β) could increase survival of ovarian cancer cells in the presence of cisplatin, we conducted a preclinical study of the antitumor effects of the TGF-β type I (TβRI) and type II (TβRII) kinase inhibitor LY2109761 in combination with cisplatin...
2015: International Journal of Clinical and Experimental Pathology
https://www.readbyqxmd.com/read/26037400/vitamin-d-levels-in-multiple-sclerosis-patients-association-with-tgf-%C3%AE-2-tgf-%C3%AE-ri-and-tgf-%C3%AE-rii-expression
#10
Zeinab Shirvani-Farsani, Mehrdad Behmanesh, Seyed Mahdi Mohammadi, Abdorreza Naser Moghadasi
AIM: A variety of evidence suggests that vitamin D can prevent the development of multiple sclerosis (MS). TGF-β pathway genes also play important roles in MS. Here, we aim to study whether vitamin D affects TGF-β pathway gene expression and Expanded Disability Status Scale (EDSS) scores in MS patients. MAIN METHODS: A randomized clinical trial was conducted on 31 relapsing-remitting (RR) MS patients. Using real-time RT-PCR, we tested the levels of TGF-β2, TGF-βRI and TGF-βRII mRNAs in the RRMS patients before and after 8 weeks of supplementation with vitamin D...
August 1, 2015: Life Sciences
https://www.readbyqxmd.com/read/26032413/spsb1-a-novel-negative-regulator-of-the-transforming-growth-factor-%C3%AE-signaling-pathway-targeting-the-type-ii-receptor
#11
Sheng Liu, Thao Nheu, Rodney Luwor, Sandra E Nicholson, Hong-Jian Zhu
Appropriate cellular signaling is essential to control cell proliferation, differentiation, and cell death. Aberrant signaling can have devastating consequences and lead to disease states, including cancer. The transforming growth factor-β (TGF-β) signaling pathway is a prominent signaling pathway that has been tightly regulated in normal cells, whereas its deregulation strongly correlates with the progression of human cancers. The regulation of the TGF-β signaling pathway involves a variety of physiological regulators...
July 17, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26025649/downregulating-hmga2-attenuates-epithelial-mesenchymal-transition-induced-invasion-and-migration-in-nasopharyngeal-cancer-cells
#12
You-You Xia, Li Yin, Ning Jiang, Wen-Jie Guo, Hao Tian, Xue-Song Jiang, Jing Wu, Meng Chen, Jian-Zhong Wu, Xia He
BACKGROUND: Epithelial-mesenchymal transition (EMT) is associated with invasion and metastasis of cancer cells. High-mobility group AT-hook 2 (HMGA2) has been found to play a critical role in EMT in a number of malignant tumors. However, whether HMGA2 regulates the EMT in human nasopharyngeal carcinoma (NPC) is unclear. OBJECTIVE: The aim of this study was to investigate the effect and mechanism of HMGA2 in inducing invasion and migration in NPC. METHODS: In NPC tissues samples, the association of HMGA2 mRNA expression with clinicopathological characteristics were estimated by real-time quantitative RT-PCR(qRT-PCR)...
July 31, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25862490/role-of-the-spleen-in-liver-regeneration-in-relation-to-transforming-growth-factor-%C3%AE-1-and-hepatocyte-growth-factor
#13
Sang Chul Lee, Hye Jin Jeong, Byung-Jo Choi, Say-June Kim
BACKGROUND: Optimizing the hepatic regenerative capacity is an immediate priority after partial hepatectomy (PH). Recent reports have indicated improvement in liver regeneration after splenectomy, raising interest on the role of the spleen in liver regeneration. However, little is known about the exact mechanism underlying these effects. MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats randomly underwent either 70% PH only (PH, n = 25) or 70% PH combined with splenectomy (PHS, n = 25)...
June 15, 2015: Journal of Surgical Research
https://www.readbyqxmd.com/read/25826292/influence-of-age-and-physical-fitness-on-mirna-21-tgf-%C3%AE-and-its-receptors-in-leukocytes-of-healthy-women
#14
COMPARATIVE STUDY
Barbara Halper, Marlene Hofmann, Stefan Oesen, Bernhard Franzke, Petra Stuparits, Claudia Vidotto, Harald Tschan, Norbert Bachl, Eva-Maria Strasser, Michael Quittan, Karl-Heinz Wagner, Barbara Wessner
The TGF-β superfamily has been shown to play an important role in a wide range of physiological as well as pathological processes including ageing, immune modulation, atherosclerosis and cancer development. The aim of the current study was to investigate (i) whether TGF-β signalling in peripheral blood mononuclear cells (PBMCs) would differ between young and old females and (ii) whether physical performance parameters of elderly women would be related to the expression of TGF-β or its receptors. Sixteen healthy young (22-28 years; YF) and 90 healthy older (65-92 years; OF) females participated in the study...
2015: Exercise Immunology Review
https://www.readbyqxmd.com/read/25813266/the-subcellular-compartmentalization-of-tgf%C3%AE-rii-and-the-dynamics-of-endosomal-formation-during-the-signaling-events-an-in-vivo-study-on-rat-mesothelial-cells
#15
Petra Balogh, Márton Magyar, Arnold Szabó, Nándor Müllner, István Likó, Attila Patócs, Anna L Kiss
We previously showed that intraperitoneal administration of Freund's adjuvant treatment resulted in acute peritonitis and TGF-β was found to be one of the main organizers of the subsequent EMT in mesothelial cells. In the present study, we investigated whether TGF-β signaling molecules are present in mesothelial cells and how their compartmentalization pattern changes with the dynamics of inflammatory events in vivo. In addition, we tried to evaluate the turnover of endosomal compartments concomitant with the internalization of signaling molecules and examine whether caveola-mediated internalization might play a role in the termination of TGF-β signaling...
May 2015: European Journal of Cell Biology
https://www.readbyqxmd.com/read/25801897/tgf%C3%AE-triggers-mir-143-145-transfer-from-smooth-muscle-cells-to-endothelial-cells-thereby-modulating-vessel-stabilization
#16
Montserrat Climent, Manuela Quintavalle, Michele Miragoli, Ju Chen, Gianluigi Condorelli, Leonardo Elia
RATIONALE: The miR-143/145 cluster is highly expressed in smooth muscle cells (SMCs), where it regulates phenotypic switch and vascular homeostasis. Whether it plays a role in neighboring endothelial cells (ECs) is still unknown. OBJECTIVE: To determine whether SMCs control EC functions through passage of miR-143 and miR-145. METHODS AND RESULTS: We used cocultures of SMCs and ECs under different conditions, as well as intact vessels to assess the transfer of miR-143 and miR-145 from one cell type to another...
May 22, 2015: Circulation Research
https://www.readbyqxmd.com/read/25707005/pluripotency-gene-expression-and-growth-control-in-cultures-of-peripheral-blood-monocytes-during-their-conversion-into-programmable-cells-of-monocytic-origin-pcmo-evidence-for-a-regulatory-role-of-autocrine-activin-and-tgf-%C3%AE
#17
Hendrik Ungefroren, Ayman Hyder, Hebke Hinz, Stephanie Groth, Hans Lange, Karim M Fawzy El-Sayed, Sabrina Ehnert, Andreas K Nüssler, Fred Fändrich, Frank Gieseler
Previous studies have shown that peripheral blood monocytes can be converted in vitro to a stem cell-like cell termed PCMO as evidenced by the re-expression of pluripotency-associated genes, transient proliferation, and the ability to adopt the phenotype of hepatocytes and insulin-producing cells upon tissue-specific differentiation. However, the regulatory interactions between cultured cells governing pluripotency and mitotic activity have remained elusive. Here we asked whether activin(s) and TGF-β(s), are involved in PCMO generation...
2015: PloS One
https://www.readbyqxmd.com/read/25631750/simvastatin-downregulates-expression-of-tgf-%C3%AE-rii-and-inhibits-proliferation-of-a549-cells-via-erk
#18
Li Shang, Shu-Shan Jia, Hai-Ming Jiang, Hua Wang, Wen-Hua Xu, Chang-Jun Lv
Lung cancer is the leading cause of cancer-related death worldwide. Transforming growth factor-β receptor II (TGF-βRII) plays an important role in the regulation of proliferation and progression in cancer. Statins have been documented to exhibit anticancer and cancer chemopreventive properties. However, the effects and mechanisms of simvastatin on the development of lung cancer are still unclear. In the present study, quiescent A549 cells were treated in vitro with fetal bovine serum (FBS) in the presence or absence of simvastatin...
June 2015: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/25606692/impaired-bone-morphogenetic-protein-receptor-ii-signaling-in-a-transforming-growth-factor-%C3%AE-dependent-mouse-model-of-pulmonary-hypertension-and-in-systemic-sclerosis
#19
Adrian J Gilbane, Emma Derrett-Smith, Sarah L Trinder, Robert B Good, Andrew Pearce, Christopher P Denton, Alan M Holmes
RATIONALE: Up to 10% of patients with systemic sclerosis (SSc) develop pulmonary arterial hypertension (PAH). This risk persists throughout the disease and is time dependent, suggesting that SSc is a susceptibility factor. Outcome for SSc-PAH is poor compared with heritable or idiopathic forms, despite clinical and pathological similarities. Although susceptibility in heritable PAH and idiopathic PAH is strongly associated with gene mutations leading to reduced expression of bone morphogenetic protein receptor (BMPR) II, these mutations have not been observed in SSc-PAH...
March 15, 2015: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/25595579/oxidative-stress-is-responsible-for-maternal-diabetes-impaired-transforming-growth-factor-beta-signaling-in-the-developing-mouse-heart
#20
Fang Wang, E Albert Reece, Peixin Yang
OBJECTIVE: Oxidative stress plays a causal role in diabetic embryopathy. Maternal diabetes induces heart defects and impaired transforming growth factor beta (TGFβ) signaling, which is essential for cardiogenesis. We hypothesize that mitigating oxidative stress through superoxide dismutase 1 (SOD1) overexpression in transgenic (Tg) mice reverses maternal hyperglycemia-impaired TGFβ signaling and its downstream effectors. STUDY DESIGN: Day 12.5 embryonic hearts from wild-type (WT) and SOD1 overexpressing embryos of nondiabetic (ND) and diabetic mellitus (DM) dams were used for the detection of oxidative stress markers: 4-hydroxynonenal (4-HNE) and malondlaldehyde (MDA), and TGFβ1, 2, and 3, phosphor (p)-TGFβ receptor II (TβRII), p-phosphorylated mothers against decapentaplegic (Smad)2, and p-Smad3...
May 2015: American Journal of Obstetrics and Gynecology
keyword
keyword
91797
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"