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https://www.readbyqxmd.com/read/29148887/wild-birds-as-a-potential-source-of-known-and-novel-multilocus-sequence-types-of-antibiotic-resistant-enterococcus-faecalis
#1
Dagmara Stępień-Pyśniak, Tomasz Hauschild, Anna Nowaczek, Agnieszka Marek, Marta Dec
We assessed the antibiotic resistance and genetic diversity of 27 Enterococcus faecalis isolates from 25 wild bird species in Poland. Resistance to lincomycin (100%) was most common followed by tetracycline (48%), erythromycin (44%), and ciprofloxacin (22%). High-level resistance to streptomycin and kanamycin was observed in 19 and 15% of isolates, respectively. One isolate (4%) exhibited low-level resistance to penicillin and vancomycin, and all isolates were susceptible to gentamicin and chloramphenicol. Antibiotic resistance was linked to the tet(M), tet(L), erm(A), erm(B), msr(A/B), ant(6)-Ia, and aph(3')-IIIa genes...
November 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/29136156/increase-in-antimicrobial-resistance-and-emergence-of-major-international-high-risk-clonal-lineages-in-dogs-and-cats-with-urinary-tract-infection-16-year-retrospective-study
#2
Cátia Marques, Adriana Belas, Andreia Franco, Catarina Aboim, Luís Telo Gama, Constança Pomba
Objectives: To evaluate temporal trends in antimicrobial resistance, over 16 years, in bacteria isolated from dogs and cats with urinary tract infection (UTI) and the clonal lineages of bacteria harbouring critical antimicrobial resistance mechanisms. Methods: Antimicrobial susceptibility testing was conducted for 948 bacteria isolated from dogs and cats with UTI (1999-2014). Resistance mechanisms were detected by PCR, namely ESBL/AmpC in third-generation cephalosporin (3GC)-resistant Escherichia coli and Proteus mirabilis, mecA in methicillin-resistant staphylococci, and aac(6')-Ieaph(2″)-Ia and aph(2″)-1d in high-level gentamicin-resistant (HLGR) enterococci...
November 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29132547/distribution-of-genes-encoding-resistance-to-aminoglycoside-modifying-enzymes-in-methicillin-resistant-staphylococcus-aureus-mrsa-strains
#3
Azar Dokht Khosravi, Atefeh Jenabi, Effat Abbasi Montazeri
Today Methicillin-Resistant Staphylococcus aureus (MRSA) have acquired multiple resistance to a wide range of antibiotics including aminoglycosides. So, this study was aimed to investigate the rate of aminoglycoside resistance and the frequency of aminoglycoside resistance mediated genes of aac(Ia)-2, aph(3)-IIIa and ant(4')-Ia among MRSA strains. A total of 467 staphylococci isolates were collected from various clinical samples. S. aureus strains were identified by standard culture and identification criteria and investigating of presence of 16S rRNA and nuc genes...
December 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29129431/ultra-rapid-catalytic-degradation-of-4-nitrophenol-with-ionic-liquid-recoverable-and-reusable-ibuprofen-derived-silver-nanoparticles
#4
Syeda Sara Hassan, Krista Carlson, Swomitra Kumar Mohanty, Sirajuddin, Ali Canlier
This study reports a one-pot and eco-friendly method for the synthesis of spherical ibuprofen derived silver nanoparticles (IBU-AgNPs) in aqueous media using ibuprofen analgesics drug as capping as well as reducing agent. Formation of AgNPs occurred within a few min (less than 5 min) at room temperature without resorting to any harsh conditions and hazardous organic solvents. Synthesized AgNPs were characterized with common analytical techniques. Transmission electron microscope (TEM) images confirmed the formation of spherical particles having a size distribution in the range of 12...
November 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29127051/genomic-analysis-of-in-vivo-acquired-resistance-to-colistin-and-rifampin-in-acinetobacter-baumannii
#5
Nadia Jaidane, Thierry Naas, Wejdene Mansour, Bechir Ben Radhia, Sofiene Jerbi, Noureddine Boujaafar, Olfa Bouallegue, Rémy A Bonnin
Acinetobacter baumannii is an opportunistic pathogen in health-care facilities responsible for nosocomial infections mostly in immune-compromised patients. Colistin resistance is increasingly reported worldwide in that species. We describe here in vivo selection of colistin and rifampin resistance in a carbapenem-resistant A. baumannii (Ab). Antimicrobial susceptibility testing, plasmid analysis, and Whole Genome Sequencing (WGS) were performed to fully characterize the resistome of AbS1 and AbS2 clinical isolates...
November 7, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29124067/labor-induction-with-orally-administrated-misoprostol-a-retrospective-cohort-study
#6
Tove Wallstrom, Hans Jarnbert-Pettersson, David Stenson, Helena Akerud, Elisabeth Darj, Kristina Gemzell-Danielsson, Eva Wiberg-Itzel
Introduction: One great challenge in obstetric care is labor inductions. Misoprostol has advantages in being cheap and stable at room temperature and available in resource-poor settings. Material and Methods: Retrospective cohort study of 4002 singleton pregnancies with a gestational age ≥34 w at Sodersjukhuset, Stockholm, during 2009-2010 and 2012-2013. Previously used methods of labor induction were compared with misoprostol given as a solution to drink, every second hour...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29109167/cloning-and-expression-of-novel-aminoglycoside-phosphotransferase-genes-from-campylobacter-and-their-role-in-the-resistance-to-six-aminoglycosides
#7
S Zhao, S Mukherjee, C Li, S B Jones, S Young, P F McDermott
Nine aph genes, including aph(2″)-Ib, Ic, Ig, If, If1, If3, Ih, aac(6')-Ie/aph(2″)-Ia, and aac(6')-Ie/aph(2″)-If2 were previously identified in Campylobacter. To measure the contribution of these alleles to aminoglycoside resistance, we cloned nine genes into the pBluescript and expressed them in E. coli DH5α. The nine aph expressed in E.coli showed varying levels of resistance to gentamicin, kanamycin and tobramycin. Three genes, aac(6″)-Ie/aph(2″)-Ia, aph2″-If1 and aph2″-Ig showed increased MICs to amikacin and five aph genes were transferrable...
November 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29091185/genomic-sequences-of-streptococcus-agalactiae-with-high-level-gentamicin-resistance-collected-in-the-bsac-bacteraemia-surveillance
#8
Michel Doumith, Shazad Mushtaq, Veronique Martin, Aiysha Chaudhry, Rachael Adkin, Juliana Coelho, Vicki Chalker, Alasdair MacGowan, Neil Woodford, David M Livermore
Background: Like other streptococci, Streptococcus agalactiae typically has intrinsic low-level aminoglycoside resistance. High-level gentamicin resistance was seen in 2 of 1125 isolates collected in the BSAC Bacteraemia Surveillance Programme between 2001 and 2014. These organisms, both isolated in 2014, were characterized. Methods: Identifications were by latex agglutination, MICs by BSAC agar dilution and sequencing by Illumina methodology. Results: Gentamicin MICs were >1024 mg/L versus a species mode of 8 mg/L; both isolates also were unusually ciprofloxacin resistant with MICs of 64 mg/L versus a species mode of 1 mg/L...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29078389/dominant-negative-effect-of-the-loss-of-function-%C3%AE-secretase-mutants-on-the-wild-type-enzyme-through-heterooligomerization
#9
Rui Zhou, Guanghui Yang, Yigong Shi
γ-secretase is an intramembrane protease complex consisting of nicastrin, presenilin-1/2, APH-1a/b, and Pen-2. Hydrolysis of the 99-residue transmembrane fragment of amyloid precursor protein (APP-C99) by γ-secretase produces β-amyloid (Aβ) peptides. Pathogenic mutations in PSEN1 and PSEN2, which encode the catalytic subunit presenilin-1/2 of γ-secretase, lead to familial Alzheimer's disease in an autosomal dominant manner. However, the underlying mechanism of how the mutant PSEN gene may affect the function of the WT allele remains to be elucidated...
October 9, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29068720/detection-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-table-eggs-in-haripur-pakistan
#10
Muhammad Ali Syed, Syeda Hala Hussain Shah, Yasmin Sherafzal, Syed Shafi-Ur-Rehman, Mushtaq Ahmed Khan, John B Barrett, Tiffanie A Woodley, Bushra Jamil, Shahid Ahmad Abbasi, Charlene R Jackson
Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria monocytogenes, and Yersinia enterocolitica have already been reported from table eggs. More important is the presence of antimicrobial-resistant bacterial strains in this food source...
October 25, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29061744/molecular-mechanisms-of-intrinsic-streptomycin-resistance-in-mycobacterium-abscessus
#11
Michael Dal Molin, Myriam Gut, Anna Rominski, Klara Haldimann, Katja Becker, Peter Sander
Streptomycin, the first drug used for the treatment of tuberculosis, shows limited activity against the highly resistant pathogen Mycobacterium abscessus We recently identified two aminoglycoside-acetylating genes [aac(2') and eis2] which however do not affect susceptibility to streptomycin. This suggests the existence of a discrete mechanism of streptomycin resistance. M. abscessus BlastP analysis identified MAB_2385 as a close homologue of the 3' '-O-phosphotransferase [APH(3' ')] from the opportunistic pathogen Mycobacterium fortuitum, as a putative streptomycin resistance determinant...
October 23, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29057497/molecular-modeling-studies-to-explore-the-binding-affinity-of-virtually-screened-inhibitor-towards-different-aminoglycoside-kinases-from-diverse-mdr-strains
#12
Rishikesh S Parulekar, Kailas D Sonawane
Antibiotic resistance to aminoglycoside group of antibiotics mainly occurs by aminoglycoside kinases (AKs). Thus, targeting AKs from different multidrug resistant (MDR) strains could result into inhibition of AK enzymes and ultimately the resistance. Therefore, the present study aims to target one of these AKs i.e. APH(3')-Ia from Acinetobacter baumannii through structure based virtual screening (SBVS) and test the binding affinity of the most stable virtually screened inhibitor with AKs from Mycobacterium tuberculosis, Acinetobacter baumannii, Enterococcus gallinarum and Escherichia coli...
October 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29055593/distribution-of-species-and-antimicrobial-resistance-among-enterococci-isolated-from-the-fecal-microbiota-of-captive-blue-fronted-parrot-amazona-aestiva-in-rio-de-janeiro-brazil
#13
Andréa de Andrade Rangel de Freitas, Adriana Rocha Faria, Tatiana de Castro Abreu Pinto, Vânia Lúcia Carreira Merquior, Daniel Marchesi Neves, Rodrigo de Cerqueira da Costa, Lúcia Martins Teixeira
Enterococcal strains recovered from fecal samples of captive blue-fronted parrots (Amazona aestiva) assisted at two wild animal screening centers in Rio de Janeiro, Brazil, were identified as Enterococcus hirae (the predominant species; 75.3%), followed by Enterococcus faecalis (17.3%), Enterococcus casseliflavus (4.8%), Enterococcus gallinarum (1.7%), and Enterococcus hermanniensis (0.9%). All strains were susceptible to linezolid and teicoplanin. Rates of nonsusceptibility (including resistant and intermediate categories) to other 16 antimicrobials tested varied from 69...
February 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29051269/management-and-long-term-outcomes-of-sarcoidosis-associated-pulmonary-hypertension
#14
Athénaïs Boucly, Vincent Cottin, Hilario Nunes, Xavier Jaïs, Abdelatif Tazi, Grégoire Prévôt, Martine Reynaud-Gaubert, Claire Dromer, Catherine Viacroze, Delphine Horeau-Langlard, Christophe Pison, Emmanuel Bergot, Julie Traclet, Jason Weatherald, Gérald Simonneau, Dominique Valeyre, David Montani, Marc Humbert, Olivier Sitbon, Laurent Savale
Studies reporting the effects of modern strategies with pulmonary arterial hypertension (PAH)-targeted therapies in sarcoidosis-associated pulmonary hypertension (S-APH) are limited.Clinical and haemodynamic data from newly diagnosed patients with severe S-APH (mean pulmonary artery pressure (mPAP) >35 mmHg or mPAP 25-35 mmHg with cardiac index <2.5 L·min(-1)·m(-2)) were collected from the French Pulmonary Hypertension Registry between 2004 and 2015.Data from 126 patients with severe S-APH were analysed (mean±sd age 57...
October 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29028569/occurrence-of-transferable-antibiotic-resistances-in-commercialized-ready-to-eat-mealworms-tenebrio-molitor-l
#15
Andrea Osimani, Federica Cardinali, Lucia Aquilanti, Cristiana Garofalo, Andrea Roncolini, Vesna Milanović, Marina Pasquini, Stefano Tavoletti, Francesca Clementi
The present study aimed to assess the occurrence of transferable determinants conferring resistance to tetracyclines, macrolide-lincosamide-streptogramin B, vancomycin, beta-lactams, and aminoglycosides in 40 samples of commercialized edible mealworms (Tenebrio molitor L.) purchased from European Union (EU) and non-EU producers. A high prevalence of tet(K) was observed in all of the samples assayed, with percentages of PCR-based positivity that ranged from 80% (samples from Thailand) to 100% (samples from the Netherlands, Belgium and France)...
October 5, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29025985/safety-biodistribution-and-radiation-dosimetry-of-68-ga-ops202-68-ga-nodaga-jr11-in-patients-with-gastroenteropancreatic-neuroendocrine-tumors-a-prospective-phase-i-imaging-study
#16
Guillaume P Nicolas, Seval Beykan, Hakim Bouterfa, Jens Kaufmann, Andreas Bauman, Michael Lassmann, Jean Claude Reubi, Jean E F Rivier, Helmut R Maecke, Melpomeni Fani, Damian Wild
Preclinical and preliminary clinical evidence indicates that radiolabeled somatostatin receptor (sst) antagonists perform better than agonists in terms of detecting neuroendocrine tumors (NETs). This prospective phase I/II study is the first to evaluate an sst antagonist, (68)Ga-OPS202 ((68)Ga-NODAGA-JR11; NODAGA=1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid and JR11=Cpa-c(DCys-Aph(Hor)-DAph(Cbm)-Lys-Thr-Cys)-DTyr-NH2)) for PET imaging. Here, we report results of the phase I component of the study...
October 12, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29025450/an-increase-in-rates-of-obstetric-haemorrhage-in-a-setting-of-high-hiv-seroprevalence
#17
E Shabalala, Hannah M Sebitloane
BACKGROUND: Obstetric haemorrhage (OH) is the leading cause of maternal mortality worldwide, although, indirectly, HIV is also a leading cause of maternal mortality in some settings with a high HIV seroprevalence. OBJECTIVE: To determine the possible association between increasing rates of OH and HIV or its treatment. METHODS: We conducted a retrospective chart review of women with OH at King Edward VIII Hospital, Durban, South Africa, over a 3-year period (2009 - 2011), during which the drug regimen for the prevention of mother-to-child transmission was evolving from single-dose nevirapine to antenatal zidovudine combined with intrapartum nevirapine (also referred to as dual therapy), and finally to a combination or highly active antiretroviral therapy (cART or HAART)...
June 30, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28986243/construction-of-an-expression-vector-that-uses-the-aph-promoter-for-protein-expression-in-corynebacterium-glutamicum
#18
Wei Zhang, Zihao Zhao, Yankun Yang, Xiuxia Liu, Zhonghu Bai
Corynebacterium glutamicum is an attractive host for the production of heterologous proteins despite its traditional use in fermentative production of amino acids. To enhance the expression levels of target genes, the development of useful promoters is required in the construction of expression systems. Here, we developed a new promoter, the aph promoter from aminoglycoside-3'-phosphotransferase gene, and used it to construct monocistronic and bicistronic expression systems that host different ribosome binding site (RBS) sequences...
October 3, 2017: Plasmid
https://www.readbyqxmd.com/read/28958590/a-novel-approach-using-metabolomics-coupled-with-hematological-and-biochemical-parameters-to-explain-the-enriching-blood-effect-and-mechanism-of-unprocessed-angelica-sinensis-and-its-4-kinds-of-processed-products
#19
Peng Ji, Yanming Wei, Yongli Hua, Xiaosong Zhang, Wanling Yao, Qi Ma, Ziwen Yuan, Yanqiao Wen, Chaoxue Yang
ETHNOPHARMACOLOGICAL RELEVANCE: Angelica sinensis (AS), root of Angelica sinensis (Oliv.) Diels, an important kind of Chinese traditional herbal medicine, has been used for women to enrich the blood for thousands of years. It is mainly distributed in Gansu province of China. According to Traditional Chinese medicine usage, unprocessed AS (UAS) and its 4 kinds of processed products (ASs) are all used to treat different diseases or syndromes. The difference among the enriching-blood effects of ASs is unclear...
September 25, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28932278/assessment-of-antibiotic-susceptibility-in-lactobacillus-isolates-from-chickens
#20
Marta Dec, Renata Urban-Chmiel, Dagmara Stępień-Pyśniak, Andrzej Wernicki
BACKGROUND: The aim of this study was to determine the susceptibility of 88 Lactobacillus isolates derived from chickens to antibiotic substances and to detect drug-resistance genes. RESULTS: The minimal inhibitory concentration of 13 antimicrobial substances was determined by the broth microdilution method, and resistance genes were detected by PCR. We recorded a high prevalence of resistance to tiamulin (90% resistant isolates), tetracyclines (74%) and lincomycin (70%), and a moderately high frequency of resistance to enrofloxacin (48%), macrolides (42%), aminoglycosides (12...
2017: Gut Pathogens
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