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https://www.readbyqxmd.com/read/28729209/clonal-spread-of-methicillin-resistant-staphylococcus-aureus-t6065-cc5-sccmecv-agrii-in-a-libyan-hospital
#1
Monia Khemiri, Ali Akrout Alhusain, Mohamed Salah Abbassi, Houyem El Ghaieb, Sofia Santos Costa, Adriana Belas, Constança Pomba, Salah Hammami
OBJECTIVES: The aim of this study was to characterize 32 MRSA isolates recovered from wound specimens of patients in a Hospital in Tripoli, Libya, during 2013. METHODS: MRSA isolates were characterized by determining their antibiotic susceptibilities, genes encoding antibiotic resistance and virulence factors, the SCCmec class, agr type, spa typing, PFGE and MLST. RESULTS: PFGE and MLST revealed that all isolates were clonal and belonged to the Clonal Complex 5 (CC5)...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28693665/characterization-of-high-level-ampicillin-and-aminoglycoside-resistant-enterococci-isolated-from-non-hospital-sources
#2
Emmanuel O Ngbede, Mashood A Raji, Clara N Kwanashie, Jacob K P Kwaga, Alex A Adikwu, Nanven A Maurice, Andrew M Adamu
PURPOSE: High level ampicillin- and aminoglycoside-resistant enterococci are being increasingly reported from non-hospital sources. This study was carried out to characterize these strains from non-hospital sources in Nigeria. METHODOLOGY: A collection of Enterococcus faecium isolated from vegetables, soil, farm animals and manure and observed to be resistant to ampicillin (n=63) and gentamicin (n=37) discs, were screened for resistance to high levels of ampicillin and aminoglycoside using E-test strips...
July 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28691828/virulence-and-antibiotic-resistance-genes-in-campylobacter-spp-in-the-czech-republic
#3
J Bardoň, V Pudová, I Koláčková, R Karpíšková, M Röderová, M Kolář
OBJECTIVE: Thermotolerant species of the genus Campy-lobacter are the important agents causing human foodborne infections throughout the world. The aims of this study were to evaluate the presence of nine putative virulence genes in Campylobacter spp. isolated from patients and from foods (poultry meat, pork liver), to determine the resistance of Campylobacter isolates to eight antibiotic agents and to detect four resistance genes.Matherial and methods: The presence of the virulence genes cdtA, cdtB, cdtC, virB11, ciaB, wlaN, iam, dnaJ and racR was detected by polymerase chain reaction (PCR) in 94 Campylobacter spp...
2017: Epidemiologie, Mikrobiologie, Imunologie
https://www.readbyqxmd.com/read/28687066/genomic-characterization-of-a-large-plasmid-containing-a-bla-ndm-1-gene-carried-on-salmonella-enterica-serovar-indiana-c629-isolate-from-china
#4
Wei Wang, Zulqarnain Baloch, Zixin Peng, Yujie Hu, Jin Xu, Séamus Fanning, Fengqin Li
BACKGROUND: The bla NDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China. METHODS: A Salmonella Indiana (S. Indiana) isolate was cultured from a chicken carcass procured from a slaughterhouse in China. Antimicrobial susceptibility was tested against a panel of agents. Whole-genome sequencing of the isolate was carried out and data was analyzed. RESULTS: A large plasmid, denoted as plasmid pC629 (210,106 bp), containing a composite cassette, consisting of IS26-bla NDM-1-ble MBL -△trpF-tat-cutA-ISCR1-sul1-qacE△1-aadA2-dfrA12-intI1-IS26 was identified...
July 7, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28684253/subinhibitory-concentrations-of-gentamicin-trigger-expression-of-aac-6-ie-aph-2%C3%A2-ia-chaperones-and-biofilm-related-genes-in-lactobacillus-plantarum-mcc3011
#5
Jaimee George, Prakash Motiram Halami
This study aimed to analyze the effects of subinhibitory concentrations of gentamicin on expression of high-level aminoglycoside-resistant (HLAR) bifunctional aac(6')-Ie-aph(2″)Ia, biofilm and chaperone genes in Lactobacillus plantarum. Analysis of biofilm formation in five isolates obtained from chicken sausages indicated their role in exhibiting phenotypic resistance based on varied MIC values, despite carrying the bifunctional gene. Biofilm formation significantly increased when L. plantarum MCC3011 was grown in subinhibitory concentrations of gentamicin (4 μg/ml), kanamycin (8 μg/ml) and streptomycin (2 μg/ml)...
July 4, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28681822/emergence-of-rmtc-and-rmtf-16s-rrna-methyltransferase-in-clinical-isolates-of-pseudomonas-aeruginosa
#6
Lavanya Mohanam, Thangam Menon
Occurrence of aminoglycoside (AG) resistance in clinical isolates of Pseudomonas aeruginosa is investigated in this study. Antimicrobial susceptibility test and minimum inhibitory concentration (MIC) for amikacin and gentamicin were performed followed by polymerase chain reaction amplifications of AG modifying enzyme genes (aac(6´)-I, aac(6´)-II, aac(3)-II/VI, ant(2´´)-I, aph(3´)-VI) and 16S methylases (rmtA-D, rmtF and armA). MIC50and MIC90were 64, 128 and > 256, >256 for amikacin and gentamicin, respectively...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28638412/molecular-identification-of-aminoglycoside-modifying-enzymes-and-plasmid-mediated-quinolone-resistance-genes-among-klebsiella-pneumoniae-clinical-isolates-recovered-from-egyptian-patients
#7
Mohamed F El-Badawy, Wael M Tawakol, Shaymaa W El-Far, Ibrahim A Maghrabi, Saleh A Al-Ghamdi, Moselhy S Mansy, Mohammed S Ashour, Mohamed M Shohayeb
Inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of Klebsiella pneumoniae. All K. pneumoniae isolates were phenotypically identified using API 20E and then confirmed genotypically through amplification of the specific K. pneumoniae phoE gene. All isolates were genotyped by the enterobacterial repetitive intergenic consensus polymerase chain reaction technique (ERIC-PCR)...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28605961/species-distribution-antibiotic-resistance-and-virulence-traits-in-canine-and-feline-enterococci-in-tunisia
#8
Leila Ben Said, Raoudha Dziri, Nadia Sassi, Carmen Lozano, Karim Ben Slama, Imen Ouzari, Carmen Torres, Naouel Klibi
In order to investigate the possible role of dogs and cats in the carriage and potential dissemination of resistant enterococci, seventy faecal samples from dogs and cats were tested for enterococci. Fifty-eight enterococci were recovered. Isolates were identified as Enterococcus faecium (n = 31) and E. faecalis (n = 14) E. durans (n = 6), E. casseliflavus (n = 2), E. hirae and E. gallinarum (2 isolates each). Enterococcal isolates showed resistance to ciprofloxacin (n = 35), erythromycin (n = 31), tetracycline (n = 25), kanamycin (n = 15), streptomycin (n = 13), pristinamycin (n = 11), gentamicin (n = 10), chloramphenicol (n = 8), and linezolid (n = 6)...
June 2017: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/28589129/whole-genome-sequence-analysis-of-antimicrobial-resistance-genes-in-streptococcus-uberis-and-streptococcus-dysgalactiae-isolates-from-canadian-dairy-herds
#9
Julián Reyes Vélez, Marguerite Cameron, Juan Carlos Rodríguez-Lecompte, Fangfang Xia, Luke C Heider, Matthew Saab, J Trenton McClure, Javier Sánchez
The objectives of this study are to determine the occurrence of antimicrobial resistance (AMR) genes using whole-genome sequence (WGS) of Streptococcus uberis (S. uberis) and Streptococcus dysgalactiae (S. dysgalactiae) isolates, recovered from dairy cows in the Canadian Maritime Provinces. A secondary objective included the exploration of the association between phenotypic AMR and the genomic characteristics (genome size, guanine-cytosine content, and occurrence of unique gene sequences). Initially, 91 isolates were sequenced, and of these isolates, 89 were assembled...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28584742/antepartum-hemorrhage-a-retrospective-analysis-from-a-northern-nigerian-teaching-hospital
#10
Idris Usman Takai, Badia Maje Sayyadi, Hadiza Shehu Galadanci
BACKGROUND: Antepartum hemorrhage (APH) contributes significantly to maternal and perinatal morbidity and mortality globally, particularly in the developing world like ours. Prevention, early detection, and prompt management cannot be overemphasized to significantly reduce the morbidity and mortality associated with this condition. OBJECTIVES: The study is aimed at determining the prevalence, etiology, sociodemographic characteristics, and the fetomaternal outcome of pregnancies complicated by APH in Aminu Kano Teaching Hospital, Kano...
April 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28577932/in-vitro-activity-of-several-antimicrobial-agents-against-methicillin-resistant-staphylococcus-aureus-mrsa-isolates-expressing-aminoglycoside-modifying-enzymes-potency-of-plazomicin-alone-and-in-combination-with-other-agents
#11
María Carmen López-Díaz, Esther Ríos, Iciar Rodríguez-Avial, Rosa Janneth Simaluiza, Juan José Picazo, Esther Culebras
This study investigated the in-vitro activity of clinically relevant aminoglycosides and new antimicrobial agents - plazomicin, ceftobiprole and dalbavancin - against 55 methicillin-resistant Staphylococcus aureus (MRSA) isolates producing aminoglycoside-modifying enzymes (AMEs). The checkerboard method was used to assess synergism between plazomicin and four antibiotics (fosfomycin, ceftobiprole, cefoxitin and meropenem), and time-kill assays were performed for the most active combinations. Among the aminoglycosides tested, plazomicin was the most active agent against MRSA, with >90% of isolates being inhibited at a minimum inhibitory concentration (MIC) of ≤1 mg/L...
May 31, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28575064/antimicrobial-resistance-of-klebsiella-pneumoniae-stool-isolates-circulating-in-kenya
#12
Chris Rowe Taitt, Tomasz A Leski, Daniel P Erwin, Elizabeth A Odundo, Nancy C Kipkemoi, Janet N Ndonye, Ronald K Kirera, Abigael N Ombogo, Judd L Walson, Patricia B Pavlinac, Christine Hulseberg, Gary J Vora
We sought to determine the genetic and phenotypic antimicrobial resistance (AMR) profiles of commensal Klebsiella spp. circulating in Kenya by testing human stool isolates of 87 K. pneumoniae and three K. oxytoca collected at eight locations. Over one-third of the isolates were resistant to ≥3 categories of antimicrobials and were considered multidrug-resistant (MDR). We then compared the resistance phenotype to the presence/absence of 238 AMR genes determined by a broad-spectrum microarray and PCR. Forty-six genes/gene families were identified conferring resistance to β-lactams (ampC/blaDHA, blaCMY/LAT, blaLEN-1, blaOKP-A/OKP-B1, blaOXA-1-like family, blaOXY-1, blaSHV, blaTEM, blaCTX-M-1 and blaCTX-M-2 families), aminoglycosides (aac(3)-III, aac(6)-Ib, aad(A1/A2), aad(A4), aph(AI), aph3/str(A), aph6/str(B), and rmtB), macrolides (mac(A), mac(B), mph(A)/mph(K)), tetracyclines (tet(A), tet(B), tet(D), tet(G)), ansamycins (arr), phenicols (catA1/cat4, floR, cmlA, cmr), fluoroquinolones (qnrS), quaternary amines (qacEΔ1), streptothricin (sat2), sulfonamides (sul1, sul2, sul3), and diaminopyrimidines (dfrA1, dfrA5, dfrA7, dfrA8, dfrA12, dfrA13/21/22/23 family, dfrA14, dfrA15, dfrA16, dfrA17)...
2017: PloS One
https://www.readbyqxmd.com/read/28569134/incidence-and-outcome-of-severe-ante-partum-hemorrhage-at-the-teaching-hospital-yalgado-ou%C3%A3-draogo-in-burkina-faso
#13
Martin Lankoande, Papougnezambo Bonkoungou, Sosthène Ouandaogo, Marcelin Dayamba, Ali Ouedraogo, Francis Veyckmans, Nazinigouba Ouédraogo
BACKGROUND: Hemorrhage is the main cause of maternal death during pregnancy. This study aims to evaluate incidence and outcomes of Severe Ante Partum Hemorrhage (SAPH) during the third trimester of pregnancy prior to delivery. METHODS: Analytical cross-sectional study with prospective data collection during 12 months in Yalgado Ouedraogo Hospital, Ouagadougou, Burkina Faso. In this context SAPH is specifically referring to Ante Partum Hemorrhage (APH) and Intra Partum Hemorrhage (IPH) in the 3rd trimester...
May 31, 2017: BMC Emergency Medicine
https://www.readbyqxmd.com/read/28483962/comparative-analysis-of-extended-spectrum-%C3%AE-lactamase-ctx-m-65-producing-salmonella-enterica-serovar-infantis-isolates-from-humans-food-animals-and-retail-chickens-in-the-united-states
#14
Heather Tate, Jason P Folster, Chih-Hao Hsu, Jessica Chen, Maria Hoffmann, Cong Li, Cesar Morales, Gregory H Tyson, Sampa Mukherjee, Allison C Brown, Alice Green, Wanda Wilson, Uday Dessai, Jason Abbott, Lavin Joseph, Jovita Haro, Sherry Ayers, Patrick F McDermott, Shaohua Zhao
We sequenced the genomes of 10 Salmonella enterica serovar Infantis isolates containing blaCTX-M-65 obtained from chicken, cattle, and human sources collected between 2012 and 2015 in the United States through routine National Antimicrobial Resistance Monitoring System (NARMS) surveillance and product sampling programs. We also completely assembled the plasmids from four of the isolates. All isolates had a D87Y mutation in the gyrA gene and harbored between 7 and 10 resistance genes [aph(4)-Ia, aac(3)-IVa, aph(3')-Ic, blaCTX-M-65, fosA3, floR, dfrA14, sul1, tetA, aadA1] located in two distinct sites of a megaplasmid (∼316 to 323 kb) similar to that described in a blaCTX-M-65-positive S Infantis isolate from a patient in Italy...
July 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28450554/biodistribution-pharmacokinetics-and-dosimetry-of-177-lu-90-y-and-111-in-labeled-somatostatin-receptor-antagonist-ops201-in-comparison-to-the-agonist-177-lu-dota-tate-the-mass-effect
#15
Guillaume Pierre Nicolas, Rosalba Mansi, Lisa McDougall, Jens Kaufmann, Hakim Bouterfa, Damian Wild, Melpomeni Fani
Radiolabeled somatostatin receptor (sstr) antagonists have shown in vivo higher uptake in sstr-expressing tumors than agonists. In this pre-clinical study the sstr2-antagonist OPS201 (DOTA-JR11; DOTA-[Cpa-c(DCys-Aph(Hor)-DAph(Cbm)-Lys-Thr-Cys)-DTyr-NH2]) labeled with (177)Lu, (90)Y and (111)In was compared with the sstr2-agonist (177)Lu-DOTA-TATE. Methods: Biodistribution, pharmacokinetics, single-photon emission computed tomography/computed tomography (SPECT/CT) and dosimetry studies were performed to assess the bioequivalence of all radiotracers...
April 27, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28413506/active-efflux-pump-adeb-is-involved-in-multidrug-resistance-of-acinetobacter-baumannii-induced-by-antibacterial-agents
#16
Tingting Zhang, Min Wang, Yixin Xie, Xianping Li, Zhihui Dong, Yanhua Liu, Ling Wang, Min Yang, Huan Song, Hong Cao, Wei Cao
The aim of the present study was to investigate the resistance of Acinetobacter baumannii, which was induced by cefepime (FEP), cefoperazone-sulbactam (SCF), tazobactam (TZP), levofloxacin (LEV), amikacin (AK), imipenem (IPM), and ciprofloxacin (CIP), in vitro. Multi-step drug resistance selection of 16 A. baumannii strains was performed using seven antibacterial agents (FEP, TZP, CIP, AK, IPM, SCF, and LEV). The minimum inhibitory concentration (MIC) was determined using the agar dilution method. Random amplified polymorphic DNA polymerase chain reaction was performed to analyze the genotypes and the carrying rates of aac(3)-I, aac(6')-I, ant(3)-I, aph(3)-Via, OXA-23, OXA-24, AmpC, TEM-1, metallo-β-lactamase gene (IMP), armA, rmtA, rmtB, parC, gyrA and adeB...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28395597/genome-sequencing-and-comparative-analysis-of-an-ndm-1-producing-klebsiella-pneumoniae-st15-isolated-from-a-refugee-patient
#17
Tamara Salloum, Harout Arabaghian, Sahar Alousi, Edmond Abboud, Sima Tokajian
The escalating problem of antibiotic resistance, specifically cabarpenemase and extended-spectrum β-lacatamase (ESBL) producing K. pneumoniae strains, is directly correlated with increased patient morbidity and mortality and prolonged hospitalization and costs. In this study, a comprehensive genomic analysis encompassing the resistomics, virulence repertoire and mobile genetic elements of an NDM-1 positive ESBL-producing K. pneumoniae EA-MEH ST15 isolated from a urine sample collected from a Syrian refugee was conducted...
June 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28373209/fludarabine-resistance-mediated-by-aminoglycoside-3-phosphotransferase-iia-and-the-structurally-related-eukaryotic-camp-dependent-protein-kinase
#18
Dámaso Sánchez-Carrera, Sara Bravo-Navas, Elena Cabezón, Ignacio Arechaga, Matilde Cabezas, Lucrecia Yáñez, Carlos Pipaón
While working with G418-resistant stably transfected cells, we realized the neomycin resistance gene (NeoR), which encodes the aminoglycoside-3'-phosphotransferase-IIa [APH(3')-IIa], also confers resistance to the nucleoside analog fludarabine. Fludarabine is a cytostatic drug widely used in the treatment of hematologic and solid tumors as well as in the conditioning of patients before transplantation of hematopoietic progenitors. We present evidence that NeoR-transfected cells do not incorporate fludarabine, thus avoiding DNA damage caused by the drug, evidenced by a lack of FANCD2 monoubiquitination and impaired apoptosis...
April 3, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28352950/effect-of-threshold-growth-as-a-major-feature-on-li-rads-categorization
#19
Victoria Chernyak, Mariya Kobi, Milana Flusberg, Kate C Fruitman, Claude B Sirlin
PURPOSE: Liver Imaging Reporting and Data System (LI-RADS) uses major features (arterial phase hyperenhancement [APHE], "washout" [WO], "capsule," diameter, threshold growth [TG]) to codify probability of hepatocellular carcinoma for each observation. This study assessed the effect of removing TG as a major feature on LI-RADS categorization. MATERIALS AND METHODS: In this HIPAA-compliant, IRB-approved study, all MR and CT clinical reports containing a standardized LI-RADS v2014 template between 4/15-1/17 were retrospectively reviewed for each LR-3, LR-4, and LR-5 reported observation...
March 28, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28339104/transferable-antibiotic-resistances-in-marketed-edible-grasshoppers-locusta-migratoria-migratorioides
#20
Andrea Osimani, Cristiana Garofalo, Lucia Aquilanti, Vesna Milanović, Federica Cardinali, Manuela Taccari, Marina Pasquini, Stefano Tavoletti, Francesca Clementi
Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70...
May 2017: Journal of Food Science
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