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https://www.readbyqxmd.com/read/28945040/-ginsenoside-rh%C3%A2-induces-apoptosis-and-autophagy-of-k562-cells-by-activating-p38
#1
Xiao-Xia Liu, Jing Xia, Jia-Feng Tang, Ming-Hua Zhou, Di-Long Chen, Ze-Hong Liu
To study the effect of ginseng saponin Rh₂ in inducing apoptosis of human leukemia K562 cells, and explore its mechanism from the aspect of autophagy pathway. CCK-8 assay was used to examine the growth inhibition of human leukemia cell lines K562 treated with ginsenoside Rh₂; flow cytometry (FCM) was used to detect cell apoptosis; Hoechst staining was used to observe the changes of cell morphological apoptosis; Acridine and MDC staining were used to detect the effects of the Rh₂ on autophagy; Western blot and RT-PCR were used to detect the expression levels of the proteins closely associated with autophagy and apoptosis...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945039/-effects-of-xuesaitong-injection-on-inflammation-induced-by-ogd-reoxygenation-in-bv2-microglia
#2
Qian Hong, Shi Wang, Chang-Xiu Chen, Yu Zhang, Yang Wang, Yuan Pan, Zeng-Chun Ma
The present study aims to investigate the effects of Xuesaitong (XST)injection on inflammation induced by OGD/reoxygenation in BV2 microglia cell and explore the underlying mechanisms.The effects of XST injection were evaluated in terms of cell viability, secretion of TNF-α, IL-1β, IL-6 and IL-10 into culture media, protein expression of p-ERK1/2, p-JNK and p-p38, and nuclear translocation of NF-κB p65. The results showed that XST injection significantly increased cell viability, suppressed release of TNF-α, IL-1β, IL-6 and IL-10 and down-regulated p-JNK1/2 and p-p38 MAPK expression in BV2 microglia cells induced by OGD/R injury, whereas it had no effect on p-ERK1/2 expression...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945037/-purification-and-cytotoxicity-of-glycoprotein-isolated-from-dendrobium-huoshanense
#3
Hui Deng, Nai-Dong Chen, Jun Dai, Nai-Fu Chen
Dendrobium huoshanense is a rare traditional Chinese medicinal herb, and the anti-tumor activity of its polysaccharides is a research hotspot in traditional Chinese medicine resources domain. This study aims to explore the material basis for the anti-tumor activity of polysaccharide. D. huoshanense was used as raw material in the experiment, and the different protein components were obtained through low salt solution extraction and ammonium sulfate fractional precipitation. Then glycoprotein components were determined by SDS-PAGE electrophoresis staining, and were further isolated and purified by DEAE ion column and Sephadex gel column...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945035/-preliminary-research-on-effect-of-licorice-processed-tripterygium-wilfordii-on-reducing-liver-toxicity
#4
Xiao-Mei Zhao, Man Gong, Jie-Ming Dong, Jia-Bo Wang, Xiao-He Xiao, Kui-Jun Zhao, Zhi-Jie Ma
To explore the effect of the licorice-processed Tripterygium wilfordii on reducing the liver toxicity. In animal experiments, the liver toxicity of T. wilfordii was evaluated both before and after processing, and the differences in liver tissue biopsy, serum biochemical indexes and inflammatory cell factor among blank group, T. wilfordii group and licorice-processed T. wilfordii group were observed. Liver tissue biopsy results showed that liver tissue injury was obvious in T. wilfordii group, and no obvious injury was found in licorice-processed T...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945028/-effects-of-methyl-jasmonate-on-accumulation-of-furanocoumarins-in-changium-smyrnioides-and-its-suspension-cells
#5
Jing Cai, Yun Ma, Jian-Wei Chen, Xiang Li, Ping Zhou
Changium smyrnioides is an endangered and endemic medicinal herb in China which contains rich furanocoumarins. Bergaptol, bergapten and xanthotoxin are natural furanocoumarins in Ch. smyrnioides, among which bergaptol is mainly contained in in vitro cultures while the latter ones distribute in all organs and cultures of the plant. In this study, methyl jasmonate was used to elicit furanocoumarins in both cultivated plant and suspension cells. The accumulations of biomass and 3 furanocoumarins as well as the activity of cell, phenylalanine ammonia-lyase and antioxidase were detected...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945024/-pathogenesis-and-treatment-of-insulin-resistance-in-chronic-kidney-disease-and-interventional-effects-of-chinese-herbal-medicine
#6
REVIEW
Wei Wu, Jing-Jing Yang, Yi-Gang Wan, Yue Tu, Ge Shi, Wen-Bei Han, Bu-Hui Liu, Jian Yao
The kidney is the target organ of insulin with abundant insulin receptors. Thereinto,the renal intrinsic cells including glomerular podocytes,endothelial cells,mesangial cells,renal tubular epitheliums and collecting duct epithelial cells are all highly sensitive to insulin as the effector cells. Furthermore,the structural and functional abnormalities of these cells are closely related to insulin and its receptors activity. It is reported that the chronic kidney disease(CKD)patients have systemic or renal insulin resistance(IR)...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945023/-research-and-application-of-hepatotoxicity-evaluation-technique-of-traditional-chinese-medicine
#7
REVIEW
Jie Song, Rong-Ling Zhong, Zhi Xia, Hao Wu, Qing-Xiang Zhong, Zhen-Hai Zhang, Ying-Jie Wei, Zi-Qi Shi, Liang Feng, Xiao-Bin Jia
The safety of traditional Chinese medicine (TCM) has received the widespread attention in recent years. Hepatotoxicity of TCM is one of the key problems of the safety of TCM. This article summarized research progress and application prospect in the mechanism of TCM hepatotoxicity, biomarkers, toxic omics database, prevention of hepatotoxicity of the liver cell lines, subcellular fraction, three-dimensional cultivation models, the model animals, aiming to provide theoretical basis for TCM toxicity evaluation and technical guidelines, thus promoting the development of TCM toxicity studies...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945015/membrane-fusion-mediated-intracellular-delivery-of-lipid-bilayer-coated-mesoporous-silica-nanoparticles
#8
Jian Yang, Jing Tu, Gerda E M Lamers, René C L Olsthoorn, Alexander Kros
Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk-shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome-c (cytC) as a model membrane-impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled-coil (CC) lipopeptides...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945013/pro-regenerative-hydrogel-restores-scarless-skin-during-cutaneous-wound-healing
#9
Guoming Sun
The transformation of fibrotic healing process to regenerative one has great potential to fully restore wounded skin. The M2 macrophage phenotype promotes constructive tissue remodeling and instructs tissue repair in a regenerative manner. It is hypothesized that hydrogels that can establish robustness of endogenous cells to regulate M2 phenotype will promote constructive dermal remodeling. Toward this end, a series of dextran-based bioabsorbable hydrogels are developed and self-crosslinkable dextran-isocyanatoethyl methacrylate-ethylamine (DexIEME) is identified as the potential scaffold...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945009/biomechanical-regulation-of-mesenchymal-stem-cells-for-cardiovascular-tissue-engineering
#10
REVIEW
Kayla Henderson, Andrew D Sligar, Victoria P Le, Jason Lee, Aaron B Baker
Mesenchymal stem cells (MSCs) are an appealing potential therapy for vascular diseases; however, many challenges remain in their clinical translation. While the use of biochemical, pharmacological, and substrate-mediated treatments to condition MSCs has been subjected to intense investigation, there has been far less exploration of using these treatments in combination with applied mechanical force for conditioning MSCs toward vascular phenotypes. This review summarizes the current understanding of the use of applied mechanical forces to differentiate MSCs into vascular cells and enhance their therapeutic potential for cardiovascular disease...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945007/nonmineralized-and-mineralized-collagen-scaffolds-induce-differential-osteogenic-signaling-pathways-in-human-mesenchymal-stem-cells
#11
Qi Zhou, Xiaoyan Ren, David Bischoff, Daniel W Weisgerber, Dean T Yamaguchi, Timothy A Miller, Brendan A C Harley, Justine C Lee
The instructive capabilities of extracellular matrix components in progenitor cell differentiation have recently generated significant interest in the development of bioinspired materials for regenerative applications. Previously, a correlation was described between the osteogenic capabilities of nanoparticulate mineralized collagen glycosaminoglycan scaffolds (MC-GAG) and an autogenous activation of small mothers against decapentaplegic ( Smad1/5) in the canonical bone morphogenetic protein receptor (BMPR) pathway with a diminished extracellular signal regulated kinase 1/2 (ERK1/2) activation when compared to nonmineralized collagen glycosaminoglycan scaffolds (Col-GAG)...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945006/microrna-let-7-targets-the-ecdysone-signaling-pathway-e75-gene-to-control-larval-pupal-development-in-bactrocera-dorsalis
#12
Wei Peng, Wei-Wei Zheng, Kaleem Tariq, Shu-Ning Yu, Hong-Yu Zhang
MicroRNAs (miRNAs) regulate various biological processes during insect development; however, their role in larval-pupal development in oriental fruit fly, Bactrocera dorsalis (Hendel) remains unknown. In current study, we address the biological function of a conserved miRNA, Bdo-Let-7 in the regulation of BdE75 gene, which belongs to ecdysone signaling pathway and participates in the larval-pupal development in B. dorsalis. Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3' UTR of BdE75 gene and suppresses its expression...
September 25, 2017: Insect Science
https://www.readbyqxmd.com/read/28945001/organ-on-a-chip-technology-for-reproducing-multiorgan-physiology
#13
REVIEW
Seung Hwan Lee, Jong Hwan Sung
In the drug development process, the accurate prediction of drug efficacy and toxicity is important in order to reduce the cost, labor, and effort involved. For this purpose, conventional 2D cell culture models are used in the early phase of drug development. However, the differences between the in vitro and the in vivo systems have caused the failure of drugs in the later phase of the drug-development process. Therefore, there is a need for a novel in vitro model system that can provide accurate information for evaluating the drug efficacy and toxicity through a closer recapitulation of the in vivo system...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944999/systemic-redistribution-of-the-intramyocardially-injected-mesenchymal-stem-cells-by-repeated-remote-ischaemic-post-conditioning
#14
Qin Jiang, Tao Yu, Keli Huang, Hao Zhang, Zhe Zheng, Shengshou Hu
We investigated the effect of repeated remote ischaemic post-conditioning (RIPoC) on the organ distribution of the intramyocardially injected MSCs in rat myocardial ischemia model. Myocardial ischemia of adult female Sprague-Dawley rats was induced by 30-min. obstruction of the left anterior descending coronary artery. Repeated RIPoC was induced after ischemia with three cycles of 5-min. occlusion and reperfusion of the limb with the frequency of half a day, 1 or 2 days, respectively. Compared with that by single RIPoC, repeated RIPoC transiently reduced oxidative stress, lipid peroxidation and inflammation in ischaemic myocardium; the gene expression of stromal cell-derived factor-1 alpha (SDF-1α) was consistently induced by repeated RIPoC procedures...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944998/enhanced-immune-response-induced-by-p5-her2-neu-derived-peptide-pulsed-dendritic-cells-as-a-preventive-cancer-vaccine
#15
Zahra Gholizadeh, Jalil Tavakkol-Afshari, Amin Reza Nikpoor, Seyed Amir Jalali, Mahmoud Reza Jaafari
Dendritic cells are special and powerful antigen-presenting cells that can induce primary immune responses against tumour-associated antigens. They can present antigens via both MHC-I and MHC-II, so they have the ability to stimulate both cytotoxic T lymphocytes and T helper cells. Furthermore, CD8(+) cytotoxic T lymphocytes require activation by CD4(+) T cells. This requires a CD4(+) T cell activator molecule, of which PADRE is one of the best. We chose an approach to use both of these important arms of the immune system...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944997/desire-for-parenthood-and-reproductive-health-knowledge-in-adolescents-and-young-adults-with-sickle-cell-disease-and-their-caregivers
#16
Leena Nahata, Nicole M Caltabellotta, Kyle Ball, Sarah H O'Brien, Susan E Creary
BACKGROUND/OBJECTIVE: Sickle cell disease (SCD) and hydroxyurea have implications for fertility and reproductive health. The goal of this study was to examine desire for parenthood and reproductive health knowledge among a cohort of adolescent and young adult (AYA) with SCD receiving hydroxyurea and their caregivers at a large pediatric academic center. METHODS: Patients with SCD were approached from September 2016 to July 2017 if they were: (1) 12-20 years old, (2) prescribed hydroxyurea for at least 6 months, (3) proficient in English, and (4) accompanied by a caregiver who was proficient in English and willing to participate...
September 25, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#17
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944988/evaluation-and-treatment-of-langerhans-cell-histiocytosis-patients-with-central-nervous-system-abnormalities-current-views-and-new-vistas
#18
REVIEW
E Ann Yeh, Jay Greenberg, Oussama Abla, Giulia Longoni, Eli Diamond, Michelle Hermiston, Brandon Tran, Carlos Rodriguez-Galindo, Carl E Allen, Kenneth L McClain
Central nervous system (CNS) involvement in Langerhans cell histiocytosis (LCH) can include mass lesions of the hypothalamic pituitary axis, choroid plexus, cerebrum, and cerebellum or magnetic resonance imaging (MRI) signal abnormalities of the cerebellum, pons, and basal ganglia. The term neurodegenerative (ND) CNS-LCH has been given to the MRI signal abnormalities and neurologic dysfunction, although initially patients may have no clinical symptoms. Standardized evaluations to better understand the natural history and response to therapy are needed...
September 25, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28944974/granulomatous-enteritis-in-rainbow-trout-oncorhynchus-mykiss-associated-with-soya-bean-meal-regardless-of-water-dissolved-oxygen-level
#19
P Mosberian-Tanha, T Landsverk, C M Press, L T Mydland, J W Schrama, M Øverland
This study investigated morphological changes associated with soya bean meal-induced enteritis (SBMIE) in distal intestine (DI) of rainbow trout (Oncorhynchus mykiss) fed a soya bean meal (SBM)-based diet and exposed to normoxia or hypoxia created by optimal and low water flow rates, respectively. A 28-day adaption period was followed by a 42-day challenge period where 600 fish were subjected to dietary challenge and/or hypoxia. Twelve tanks each containing 50 juvenile trout were assigned randomly in triplicate to each treatment...
September 25, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28944964/wnt-%C3%AE-catenin-and-erk-pathway-activation-a-possible-mechanism-of-photobiomodulation-therapy-with-light-emitting-diodes-that-regulate-the-proliferation-of-human-outer-root-sheath-cells
#20
Jung E Kim, Young J Woo, Ki M Sohn, Kwan H Jeong, Hoon Kang
BACKGROUND: Outer root sheath cells (ORSCs) play important roles in maintaining hair follicle structure and provide support for the bulge area. The hair growth promoting effects of photobiomodulation therapy (PBMT) have been reported, but the mechanisms for this in human ORCs (hORSCs) have rarely been studied. OBJECTIVE: The aim of this study was to investigate the effect of various wavelengths of light-emitting diode (LED) irradiation on human ORSCs (hORSCs). METHODS: LED irradiation effects on hORSC proliferation and migration were examined with MTT assay, BrdU incorporation assay and migration assays...
September 25, 2017: Lasers in Surgery and Medicine
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