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https://www.readbyqxmd.com/read/28645053/assessment-of-imaging-in-flow-system-flowcam-for-systematic-ballast-water-management
#1
Leonardo Romero-Martínez, Cees van Slooten, Enrique Nebot, Asunción Acevedo-Merino, Louis Peperzak
Assessing the disinfection of ballast water and its compliance with international standards requires determining the size, viability, and concentration of planktonic organisms. The FlowCAM (Flow Cytometer and Microscope) is an Imaging Flow Cytometry designed to obtain the particle concentration, images, and quantitative morphologic information. The objective in this paper is to establish the basis for transforming the FlowCAM from being a laboratory analyzer into a tool for systematic monitoring of ballast water...
June 20, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28644546/-18-f-fluoromisonidazole-uptake-in-advanced-stage-non-small-cell-lung-cancer-a-voxel-by-voxel-pet-kinetics-study
#2
Daniel R McGowan, Ruth E Macpherson, Sara L Hackett, Dan Liu, Fergus V Gleeson, W Gillies McKenna, Geoff S Higgins, John D Fenwick
PURPOSE: To determine the relative abilities of compartment models to describe time-courses of 18F-fluoromisonidazole (FMISO) uptake in tumor voxels of patients with non-small cell lung cancer (NSCLC) imaged using dynamic positron emission tomography. Also to use fits of the best-performing model to investigate changes in fitted rate-constants with distance from the tumor edge. METHODS: Reversible and irreversible two- and three-tissue compartment models were fitted to 24,662 individual voxel time activity curves (TACs) obtained from tumors in 9 patients, each imaged twice...
June 23, 2017: Medical Physics
https://www.readbyqxmd.com/read/28643891/direct-comparison-of-high-temporal-resolution-cine-mri-with-doppler-ultrasound-for-assessment-of-diastolic-dysfunction-in-mice
#3
Thomas A Roberts, Anthony N Price, Laurence H Jackson, Valerie Taylor, Anna L David, Mark F Lythgoe, Daniel J Stuckey
Diastolic dysfunction is a sensitive early indicator of heart failure and can provide additional data to conventional measures of systolic function. Transmitral Doppler ultrasound, which measures the one-dimensional flow of blood through the mitral valve, is currently the preferred method for the measurement of diastolic function, but the measurement of the left ventricular volume changes using high-temporal-resolution cinematic magnetic resonance imaging (CINE MRI) is an alternative approach which is emerging as a potentially more robust and user-independent technique...
June 23, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28643821/non-invasive-approaches-in-the-diagnosis-of-acute-rejection-in-kidney-transplant-recipients-part-i-in-vivo-imaging-methods
#4
REVIEW
Oriane Hanssen, Pauline Erpicum, Pierre Lovinfosse, Paul Meunier, Laurent Weekers, Luaba Tshibanda, Jean-Marie Krzesinski, Roland Hustinx, François Jouret
Kidney transplantation (KTx) represents the best available treatment for patients with end-stage renal disease. Still, full benefits of KTx are undermined by acute rejection (AR). The diagnosis of AR ultimately relies on transplant needle biopsy. However, such an invasive procedure is associated with a significant risk of complications and is limited by sampling error and interobserver variability. In the present review, we summarize the current literature about non-invasive approaches for the diagnosis of AR in kidney transplant recipients (KTRs), including in vivo imaging, gene expression profiling and omics analyses of blood and urine samples...
February 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28643819/non-invasive-approaches-in-the-diagnosis-of-acute-rejection-in-kidney-transplant-recipients-part-ii-omics-analyses-of-urine-and-blood-samples
#5
REVIEW
Pauline Erpicum, Oriane Hanssen, Laurent Weekers, Pierre Lovinfosse, Paul Meunier, Luaba Tshibanda, Jean-Marie Krzesinski, Roland Hustinx, François Jouret
Kidney transplantation (KTx) represents the best available treatment for patients with end-stage renal disease. Still, the full benefits of KTx are undermined by acute rejection (AR). The diagnosis of AR ultimately relies on transplant needle biopsy. However, such an invasive procedure is associated with a significant risk of complications and is limited by sampling error and interobserver variability. In the present review, we summarize the current literature about non-invasive approaches for the diagnosis of AR in kidney transplant recipients (KTRs), including in vivo imaging, gene-expression profiling and omics analyses of blood and urine samples...
February 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28643041/skull-fracture-with-effacement-of-the-superior-sagittal-sinus-following-drone-impact-a-case-report
#6
Lawrance K Chung, Yuri Cheung, Carlito Lagman, Nicholas Au Yong, Duncan Q McBride, Isaac Yang
BACKGROUND: The popularity of unmanned aerial vehicles, or drones, raises safety concerns as they become increasingly common for commercial, personal, and recreational use. Collisions between drones and people may result in serious injuries. CASE REPORT: A 13-year-old male presented with a comminuted depressed skull fracture causing effacement of the superior sagittal sinus secondary to a racing drone impact. The patient experienced a brief loss of consciousness and reported lower extremity numbness and weakness after the accident...
June 22, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28642995/accuracy-and-reproducibility-of-fast-fractional-flow-reserve-computation-from-invasive-coronary-angiography
#7
A R van Rosendael, G Koning, A C Dimitriu-Leen, J M Smit, J M Montero-Cabezas, F van der Kley, J W Jukema, J H C Reiber, J J Bax, A J H A Scholte
Fractional flow reserve (FFR) guided percutaneous coronary intervention (PCI) is associated with favourable outcome compared with revascularization based on angiographic stenosis severity alone. The feasibility of the new image-based quantitative flow ratio (QFR) assessed from 3D quantitative coronary angiography (QCA) and thrombolysis in myocardial infarction (TIMI) frame count using three different flow models has been reported recently. The aim of the current study was to assess the accuracy, and in particular, the reproducibility of these three QFR techniques when compared with invasive FFR...
June 22, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28642699/cerebral-blood-flow-and-amyloid-%C3%AE-interact-to-affect-memory-performance-in-cognitively-normal-older-adults
#8
Katherine J Bangen, Alexandra L Clark, Emily C Edmonds, Nicole D Evangelista, Madeleine L Werhane, Kelsey R Thomas, Lyzette E Locano, My Tran, Zvinka Z Zlatar, Daniel A Nation, Mark W Bondi, Lisa Delano-Wood
Cerebral blood flow (CBF) alterations and amyloid-β (Aβ) accumulation have been independently linked to cognitive deficits in older adults at risk for dementia. Less is known about how CBF and Aβ may interact to affect cognition in cognitively normal older adults. Therefore, we examined potential statistical interactions between CBF and Aβ status in regions typically affected in Alzheimer's disease (AD) within a sample of older adults from the Alzheimer's Disease Neuroimaging Initiative (ADNI) study. Sixty-two cognitively normal participants (mean age = 72 years) underwent neuroimaging and memory testing...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28642468/metal-vapor-micro-jet-controls-material-redistribution-in-laser-powder-bed-fusion-additive-manufacturing
#9
Sonny Ly, Alexander M Rubenchik, Saad A Khairallah, Gabe Guss, Manyalibo J Matthews
The results of detailed experiments and finite element modeling of metal micro-droplet motion associated with metal additive manufacturing (AM) processes are presented. Ultra high speed imaging of melt pool dynamics reveals that the dominant mechanism leading to micro-droplet ejection in a laser powder bed fusion AM is not from laser induced recoil pressure as is widely believed and found in laser welding processes, but rather from vapor driven entrainment of micro-particles by an ambient gas flow. The physics of droplet ejection under strong evaporative flow is described using simulations of the laser powder bed interactions to elucidate the experimental results...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642434/on-the-generation-of-solar-spicules-and-alfv%C3%A3-nic-waves
#10
J Martínez-Sykora, B De Pontieu, V H Hansteen, L Rouppe van der Voort, M Carlsson, T M D Pereira
In the lower solar atmosphere, the chromosphere is permeated by jets known as spicules, in which plasma is propelled at speeds of 50 to 150 kilometers per second into the corona. The origin of the spicules is poorly understood, although they are expected to play a role in heating the million-degree corona and are associated with Alfvénic waves that help drive the solar wind. We compare magnetohydrodynamic simulations of spicules with observations from the Interface Region Imaging Spectrograph and the Swedish 1-m Solar Telescope...
June 23, 2017: Science
https://www.readbyqxmd.com/read/28641615/-expression-and-asymmetric-division-of-numb-in-leukemia-cell-k562-line
#11
Zheng Li, Huan Li, Yi-Hui Li, Ying-Xi Xu, Hai-Yan Xing, Ke-Jing Tang, Zheng Tian, Min Wang, Qing Rao
OBJECTIVE: To investigate the role of asymmetric division in leukemia cells through detection of expression and asymmetric division of Numb in differentiated and undifferentiated K562 cells. METHODS: Firstly, Hemin was used to induce K562 cell differentiation, and the expression of Numb was detected by the real-time quantitative RT-PCR and flow cytometry. After K562 cells were synchronized by nocodazole, the Numb protein was labeled by immunohistochemical staining, followed by the determination of the terminally differentiated cells through confocal microscopy...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28641192/obstructive-neonatal-atrial-myxoma
#12
Federica Iezzi, Andrea Quarti, Alessandro Capestro, Marco Pozzi
INTRODUCTION: Cardiac myxoma is a benign neoplasm representing the most common primary cardiac tumor in adults, however it is unusual in neonates. It is represented by an endocardial mass that occupies the cardiac chamber. Although the majority of myxomas are attached to the fossa ovalis of the interatrial septum, they also attach to the walls of the cardiac chambers and to valve leaflets surfaces. Approximately 75% of myxomas are found in the left atrium, 20% are located in the right atrium, and rarely in the ventricles...
June 8, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28640847/heart-rate-lowering-treatment-leads-to-a-reduction-in-vulnerable-plaque-features-in-atherosclerotic-rabbits
#13
Raf H M van Hoof, Evelien Hermeling, Judith C Sluimer, Julie Salzmann, Arnold P G Hoeks, Jérôme Roussel, Mat J A P Daemen, Harry Struijker-Boudier, Joachim E Wildberger, Sylvia Heeneman, M Eline Kooi
OBJECTIVE: To investigate the effect of a heart rate (HR) lowering agent (Ivabradine) on features of atherosclerotic plaque vulnerability with magnetic resonance imaging (MRI), ultrasound imaging, and histology. APPROACH AND RESULTS: Atherosclerosis was induced in the abdominal aorta of 19 rabbits. Nine rabbits were treated with Ivabradine (17 mg/kg/day) during the entire study period. At week 14, imaging was performed. Plaque size was quantified on contrast-enhanced T1-weighted MR images...
2017: PloS One
https://www.readbyqxmd.com/read/28640793/intraoperative-computed-tomography-ct-for-treating-giant-carotid-intracavernous-aneurysms
#14
Zhe Xue, Fuyu Wang, Zhenghui Sun, Hui Zhang, Chen Wu, Dongsheng Kong, Bainan Xu
BACKGROUND Giant carotid intracavernous aneurysm refers to those lesions larger than 2.5 cm and derived from a cavernous segment, accounting for about 30% of all intracranial tumors. Dynamic CT perfusion imaging (PCT) is a common method recently employed to evaluate cerebral perfusion. This study investigated the efficacy and clinical application of intraoperative CT in the surgery for giant symptomatic carotid intracavernous aneurysm. MATERIAL AND METHODS A retrospective analysis was performed on 23 cases with giant symptomatic carotid intracavernous aneurysm...
June 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28640518/a-carborane-containing-fluorophore-as-a-stain-of-cellular-lipid-droplets
#15
Andrew Wu, Jacek L Kolanowski, Badwi B Boumelhem, Kylie Yang, Rebecca Lee, Amandeep Kaur, Stuart T Fraser, Elizabeth J New, Louis M Rendina
The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane-containing coumarin, 5, which is sufficiently lipophilic to localise in cellular lipid droplets. In non-polar solvents which show comparable polarities to those of a lipid environment, compound 5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging...
June 22, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28640394/in-scan-and-scan-rescan-assessment-of-lv-in-and-outflow-volumes-by-4d-flow-mri-versus-2d-planimetry
#16
Vivian P Kamphuis, Roel L F van der Palen, Patrick J H de Koning, Mohammed S M Elbaz, Rob J van der Geest, Albert de Roos, Arno A W Roest, Jos J M Westenberg
PURPOSE: To evaluate the in-scan and scan-rescan consistency of left ventricular (LV) in- and outflow assessment from 1) 2D planimetry; 2) 4D flow magnetic resonance imaging (MRI) with retrospective valve tracking, and 3) 4D flow MRI with particle tracing. MATERIALS AND METHODS: Ten healthy volunteers (age 27 ± 3 years) underwent multislice cine short-axis planimetry and whole-heart 4D flow MRI on a 3T MRI scanner twice with repositioning between the scans...
June 22, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28639568/intraoperative-transesophageal-echocardiography-in-the-operation-of-ebstein-s-anomaly-a-retrospective-study
#17
Xiu-Jie Tang, Min Bao, He Zhao, Lian-Yi Wang, Qing-Yu Wu
BACKGROUND: Ebstein's anomaly (EA) has various spectrums in clinical and anatomic features. This study aimed to report the experience of two-dimensional intraoperative transesophageal echocardiography (2D-ITEE) during the EA surgery and to analyze the characteristics of the tricuspid valve (TV) by comparing the data from 2D-ITEE with the results from the surgery. METHODS: 2D-ITEE data of 164 patients with EA who were operated in the First Hospital of Tsinghua University between July 2004 and April 2014 were retrospectively analyzed in this study...
July 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28639328/immunogenicity-of-protein-aggregates-of-a-monoclonal-antibody-generated-by-forced-shaking-stress-with-siliconized-and-nonsiliconized-syringes-in-balb-c-mice
#18
Tomonobu Uchino, Yasunori Miyazaki, Takuto Yamazaki, Yoshiyuki Kagawa
OBJECTIVE: In this study, we aimed to investigate the immunogenicity of protein aggregates of monoclonal antibodies (mAbs), generated by forced shaking stress with siliconized and nonsiliconized syringes in a mouse model. METHODS: Samples were filled in siliconized and nonsiliconized syringes with shaking and headspace air. Characterization studies were performed using high-performance size-exclusion chromatography, nanoparticle tracking analysis, flow cytometry, micro-flow imaging and resonant mass measurement...
June 21, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28638829/mitochondrial-targeted-molecular-imaging-in-cardiac-disease
#19
REVIEW
Jinhui Li, Jing Lu, You Zhou
The present study aimed to discuss the role of mitochondrion in cardiac function and disease. The mitochondrion plays a fundamental role in cellular processes ranging from metabolism to apoptosis. The mitochondrial-targeted molecular imaging could potentially illustrate changes in global and regional cardiac dysfunction. The collective changes that occur in mitochondrial-targeted molecular imaging probes have been widely explored and developed. As probes currently used in the preclinical setting still have a lot of shortcomings, the development of myocardial metabolic activity, viability, perfusion, and blood flow molecular imaging probes holds great potential for accurately evaluating the myocardial viability and functional reserve...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28638804/pkc-%C3%AE-marcks-signaling-promotes-intracellular-survival-of-unopsonized-burkholderia-thailandensis
#20
Sofiya N Micheva-Viteva, Yulin Shou, Kumkum Ganguly, Terry H Wu, Elizabeth Hong-Geller
Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic treatment. To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen of the human kinome and identified 35 host kinases that facilitated Burkholderia thailandensis intracellular survival in human monocytic THP-1 cells. We validated a selection of host kinases using imaging flow cytometry to assess efficiency of B. thailandensis survival in the host upon siRNA-mediated knockdown...
2017: Frontiers in Cellular and Infection Microbiology
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