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Cometic Surgery

Yong Wang, Junfang Zhang, Jian Wu, Huaijin Guan
The formation and development of age-related cataract (ARC) has been demonstrated to have the involvement of defective DNA repair in lens epithelial cells (LECs). This study aimed to investigate DNA repair genes expression in human lens cortex collected from age-related cortical cataract (ARCC) and controls during surgery. The expression levels of the genes were evaluated by xx genes microarray analysis. The results were further confirmed by Quantitative Real-Time PCR (qRT-PCR). The mRNA levels of 7 genes decreased and 4 genes out of 92 genes increased in lens cortex of ARCCs compared with controls with the fold change >1...
February 12, 2017: Experimental and Molecular Pathology
Hong-Wei Wang, Le-Yi Wang, Li Jiang, Su-Ming Tian, Tai-Di Zhong, Xiang-Ming Fang
BACKGROUND: A retrospective analysis of patients undergoing cancer surgery suggested that using local anesthetics could reduce cancer recurrence and improve survival rate. Previous studies have indicated that local anesthetics may induce apoptosis in several kinds of cells in vitro, but the mechanism is unclear. METHODS: Cell viability was analyzed by MTS; reactive oxygen species (ROS), mitochondrial membrane potential (MMP, ∆Ψm), cell cycle distribution, and cell apoptosis assay were detected by flow cytometry; DNA damage was measured by comet assay; cell invasion and migration were observed by microscopy; The expression level of related proteins was detected by western blot assay...
October 2016: Journal of Thoracic Disease
Kingsley Powell, Victoria J Wilson, Niamh M Redmond, Daisy M Gaunt, Matthew J Ridd
BACKGROUND: Recruiting to target in randomised controlled trials of investigational medicinal products (CTIMPs) in primary care and paediatric populations is notoriously difficult. More evidence is needed for effective recruitment strategies in these settings. We report on the impact of different recruitment strategies used in the Choice of Moisturiser in Eczema Treatment (COMET) study - a feasibility trial comparing the effectiveness of four emollients for the treatment of childhood eczema - recruiting via general practitioner (GP) surgeries...
November 17, 2016: Trials
Hongxing Ye, Mantao Chen, Fei Cao, Hongguang Huang, Renya Zhan, Xiujue Zheng
BACKGROUND: Glioblastoma is refractory to conventional treatment, which is combined of surgery, chemotherapy and radiotherapy. Recent studies have shown that glioma initiating cells (GICs) contribute to tumorigenesis and radioresistance. Recently, other studies showed that the GICs use the autophagy as the major pathway to survive. Chloroquine, an anti-malarial chemical, is an autophagic inhibitor which blocks autophagosome fusion with lysosome and slows down lysosomal acidification. The aim of this study was to explore the mechanisms of chloroquine on the radiosensitivity of GICs...
September 20, 2016: BMC Neurology
Pinnita Prabhasawat, Pattama Ekpo, Mongkol Uiprasertkul, Suksri Chotikavanich, Nattaporn Tesavibul, Kanograt Pornpanich, Panitee Luemsamran
The present study aimed to investigate the clinical outcomes of autologous cultivated oral mucosal epithelial transplantation (COMET) on human amniotic membrane (AM) for corneal limbal stem cell deficiency (LSCD). In this prospective, noncomparative case series, 20 eyes (18 patients) with bilateral severe ocular surface disease were chosen to undergo COMET on human AM. The primary outcome was clinical success, and the secondary outcomes were the best-corrected visual acuity difference, corneal opacification, symblepharon formation, and complications...
September 2016: Cell and Tissue Banking
Flávia R Nogueira, Leandro G Braz, Leonardo R de Andrade, Ana Lygia R de Carvalho, Luiz A Vane, Norma Sueli P Módolo, Aline G Aun, Kátina M Souza, José Reinaldo C Braz, Mariana G Braz
There is controversy over the genotoxic effects of volatile anesthetics. The available literature on the genotoxicity of desflurane, one of the newest volatile halogenated agents used for general anesthesia maintenance, is scarce. This study aimed to evaluate the genotoxic potential of desflurane in 15 patients without comorbidities, of both sexes, who underwent minor surgeries lasting at least 90 min. Patients enrolled in the study received desflurane anesthesia (6%); blood samples were collected before anesthesia induction (T0), 90 min after the beginning of anesthesia (T1), and on the day following surgery (T2)...
May 2016: Environmental and Molecular Mutagenesis
A Moza, C Benstoem, R Autschbach, C Stoppe, A Goetzenich
BACKGROUND: Cardiovascular disease (CVD) is a major contributor to the burden of disease and the number one cause of death worldwide. From 1990 until today, more people died from coronary heart disease than from any other cause. CVD is regularly treated with minimally or non-minimally invasive off- or on-pump cardiothoracic surgery and several interventions related to the outcome of the surgical procedures have been evaluated in clinical trials, but heterogeneity in outcome reporting hinders comparison of interventions across trials and limits the ability of research synthesis...
2015: Trials
Francielli Licks, Renata Minuzzo Hartmann, Camila Marques, Elizângela Schemitt, Josieli Raskopf Colares, Mariana do Couto Soares, Juliana Reys, Camila Fisher, Juliana da Silva, Norma Possa Marroni
AIM: To evaluate the antioxidant effect of N-acetylcysteine (NAC) on the stomach of rats with portal hypertension. METHODS: Twenty-four male Wistar rats weighing ± 250 g were divided into four experimental groups (n = 6 each): Sham-operated (SO), SO + NAC, partial portal vein ligation (PPVL), and PPVL + NAC. Treatment with NAC in a dose of 10 mg/kg (i.p.) diluted in 0.6 mL of saline solution was administered daily for 7 d starting 8 d after the surgery. Animals from the PPVL and SO group received saline solution (0...
November 21, 2015: World Journal of Gastroenterology: WJG
Asmund Avdem Fretland, Andrey Sokolov, Nadya Postriganova, Airazat M Kazaryan, Soren E Pischke, Per H Nilsson, Ingrid Nygren Rognes, Bjorn Atle Bjornbeth, Morten Wang Fagerland, Tom Eirik Mollnes, Bjorn Edwin
Laparoscopic and open liver resection have not been compared in randomized trials. The aim of the current study was to compare the inflammatory response after laparoscopic and open resection of colorectal liver metastases (CLM) in a randomized controlled trial.This was a predefined exploratory substudy within the Oslo CoMet-study. Forty-five patients with CLM were randomized to laparoscopic (n = 23) or open (n = 22) resection. Ethylenediaminetetraacetic acid-plasma samples were collected preoperatively and at defined time points during and after surgery and snap frozen at -80 C...
October 2015: Medicine (Baltimore)
Dariusz Dobrowolski, Boguslawa Orzechowska-Wylegala, Bogumil Wowra, Ewa Wroblewska-Czajka, Maria Grolik, Krzysztof Szczubialka, Maria Nowakowska, Domenico Puzzolo, Edward A Wylegala, Antonio Micali, Pasquale Aragona
PURPOSE: Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients. METHODS: Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane...
2015: BioMed Research International
Young Sub Lee, Yourha Kim, Sora Jeon, Ja Seong Bae, So Lyung Jung, Chan Kwon Jung
The hobnail variant of papillary thyroid carcinoma (PTC) is a rare, aggressive variant in which > 30% of the tumor cells have hobnail features. The clinical behavior and pathologic characteristics of these tumors are still unclear due to the rarity of the entity. The present study aimed to investigate cytologic, clinical, pathological, and molecular features of the hobnail variant from our data and from the literature. We retrospectively retrieved 10 cases of hobnail variant from 2,904 consecutive PTC patients...
2015: International Journal of Clinical and Experimental Pathology
Mariana G Braz, Leandro G Braz, Cristiana M M Freire, Lorena M C Lucio, José R C Braz, Guangwen Tang, Daisy M F Salvadori, Kyung-Jin Yeum
Isoflurane is a volatile halogenated anesthetic used especially for anesthesia maintenance whereas propofol is a venous anesthetic utilized for anesthesia induction and maintenance, and reportedly an antioxidant. However, there are still controversies related to isoflurane-induced oxidative stress and it remains unanswered whether the antioxidant effects occur in patients under propofol anesthesia.Taking into account the importance of better understanding the role of anesthetics on oxidative stress in anesthetized patients, the present study was designed to evaluate general anesthesia maintained with isoflurane or propofol on antioxidant status in patients who underwent minimally invasive surgeries...
August 2015: Medicine (Baltimore)
Zbigniew Adamczewski, Mariusz Stasiołek, Bolesław Karwowski, Marek Dedecjus, Daria Orszulak-Michalak, Anna Merecz, Przemysław W Śliwka, Bartosz Puła, Andrzej Lewiński
BACKGROUND: Administration of diagnostic activities of 131I, performed in order to detect thyroid remnants after surgery and/or thyroid cancer recurrence/metastases, may lead to reduction of iodine uptake. This phenomenon is called "thyroid stunning". We estimated radiation absorbed dose-dependent changes in genetic material, in particular in sodium iodide symporter (NIS) gene promoter, and NIS protein level in human thyrocytes (HT). MATERIALS AND METHODS: We used unmodified HT isolated from patients subjected to thyroidectomy exposed to 131I in culture...
2015: International Journal of Molecular Sciences
Åsmund Avdem Fretland, Airazat M Kazaryan, Bjørn Atle Bjørnbeth, Kjersti Flatmark, Marit Helen Andersen, Tor Inge Tønnessen, Gudrun Maria Waaler Bjørnelv, Morten Wang Fagerland, Ronny Kristiansen, Karl Øyri, Bjørn Edwin
BACKGROUND: Laparoscopic liver resection is used in specialized centers all over the world. However, laparoscopic liver resection has never been compared with open liver resection in a prospective, randomized trial. METHODS/DESIGN: The Oslo-CoMet Study is a randomized trial into laparoscopic versus open liver resection for the surgical management of hepatic colorectal metastases. The primary outcome is 30-day perioperative morbidity. Secondary outcomes include 5-year survival (overall, disease-free and recurrence-free), resection margins, recurrence pattern, postoperative pain, health-related quality of life, and evaluation of the inflammatory response...
March 4, 2015: Trials
Andreas Lambertz, Kai Michael Schröder, Dominik Stefan Schöb, Marcel Binnebösel, Michael Anurov, Uwe Klinge, Ulf Peter Neumann, Christian Daniel Klink
BACKGROUND: Biocompatibility and tissue integration of a surgical suture are decisive factors for wound healing and therefore for the success of sutures. The optimal suture material is still under discussion. Polyvinylidene fluoride (PVDF) is described to have superior properties of biocompatibility and is therefore frequently used as a mesh component. Only little information is available about its use as a suture material. The aim of this study was to evaluate the biocompatibility of PVDF as a suture material in comparison to 5 different established sutures in a rat model...
2015: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
Beryl R Benacerraf, Yvette Groszmann, Mark D Hornstein, Bryann Bromley
The purpose of this study was to evaluate the appearance of deep infiltrating endometriosis of the bowel wall in the cul-de-sac and determine the characteristic appearance of these lesions. We searched our database between January 1, 2011, and December 31, 2013, for all patients who had sonographic findings of suspected deep infiltrating endometriosis of the bowel with obliteration of the cul-de-sac. The medical record of each patient was examined retrospectively for evidence of surgical confirmation of disease...
March 2015: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
Dimitra T Stefanou, Aristotelis Bamias, Hara Episkopou, Soterios A Kyrtopoulos, Maria Likka, Theodore Kalampokas, Stylianos Photiou, Nikos Gavalas, Petros P Sfikakis, Meletios A Dimopoulos, Vassilis L Souliotis
Ovarian carcinoma (OC) is the most lethal gynecological malignancy. Despite the advances in the treatment of OC with combinatorial regimens, including surgery and platinum-based chemotherapy, patients generally exhibit poor prognosis due to high chemotherapy resistance. Herein, we tested the hypothesis that DNA damage response (DDR) pathways are involved in resistance of OC patients to platinum chemotherapy. Selected DDR signals were evaluated in two human ovarian carcinoma cell lines, one sensitive (A2780) and one resistant (A2780/C30) to platinum treatment as well as in peripheral blood mononuclear cells (PBMCs) from OC patients, sensitive (n = 7) or resistant (n = 4) to subsequent chemotherapy...
2015: PloS One
Sana M Salih, Ashley K Ringelstetter, Mazin Z Elsarrag, David H Abbott, Elon C Roti Roti
Preservation of ovarian function following chemotherapy for nonovarian cancers is a formidable challenge. For prepubescent girls, the only option to prevent chemotherapy damage to the ovary is ovarian tissue cryopreservation, an experimental procedure requiring invasive surgeries to harvest and reimplant tissue, which carries the risk of cancer reintroduction. Drugs that block the primary mechanism of chemotherapy insult, such as dexrazoxane (Dexra) in the context of anthracycline chemotherapy, provide a novel approach for ovarian protection and have the potential to overcome current limitations to oncofertility treatment...
March 2015: Biology of Reproduction
D A Jones, S L Prior, J D Barry, S Caplin, J N Baxter, J W Stephens
AIMS: In the past 30 years, prevalence of obesity has almost trebled resulting in an increased incidence of type 2 diabetes mellitus and other co-morbidities. Visceral adipose tissue is believed to play a vital role, but underlying mechanisms remain unclear. Our aim was to investigate changes in markers of oxidative damage in human visceral adipose tissue to determine levels of oxidative burden that may be attributed to obesity and/or diabetes. METHODS: Visceral adipose tissue samples from 61 subjects undergoing abdominal surgery grouped as lean, obese and obese with type 2 diabetes mellitus, were examined using 3 different markers of oxidative stress...
December 2014: Diabetes Research and Clinical Practice
José Eduardo B Orosz, Leandro G Braz, Ana Lucia A Ferreira, Rosa Beatriz Amorim, Daisy Maria F Salvadori, Kyung-Jin Yeum, José Reinaldo C Braz, Mariana G Braz
Despite the effectiveness and safety of anesthetics, some unanswered questions remain concerning their toxicity and effects on cellular redox balance. To test for possible toxic effects of balanced anesthesia maintained with the volatile anesthetic sevoflurane, we evaluated oxidative stress during and after general anesthesia in 15 adult patients without comorbidities who underwent elective minor surgical procedures. Venous blood samples were collected at baseline, before anesthesia (t0); after anesthesia induction and immediately before surgery (t1); 2h after the beginning of anesthesia (t2); and on the day following surgery (t3)...
October 2014: Mutation Research. Genetic Toxicology and Environmental Mutagenesis
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