Priyanka Patel, Courtney N Buchanan, Matthew D Zdradzinski, Pabitra K Sahoo, Amar N Kar, Seung Joon Lee, Lauren S Vaughn, Anatoly Urisman, Juan Oses-Prieto, Michela Dell'Orco, Devon E Cassidy, Irene Dalla Costa, Sharmina Miller, Elizabeth Thames, Terika P Smith, Alma L Burlingame, Nora Perrone-Bizzozero, Jeffery L Twiss
Axonally synthesized proteins support nerve regeneration through retrograde signaling and local growth mechanisms. RNA binding proteins (RBP) are needed for this and other aspects of post-transcriptional regulation of neuronal mRNAs, but only a limited number of axonal RBPs are known. We used targeted proteomics to profile RBPs in peripheral nerve axons. We detected 76 proteins with reported RNA binding activity in axoplasm, and levels of several change with axon injury and regeneration. RBPs with altered levels include KHSRP that decreases neurite outgrowth in developing CNS neurons...
June 10, 2022: Nucleic Acids Research