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https://www.readbyqxmd.com/read/28746169/traditional-long-term-central-venous-catheters-versus-transhepatic-venous-catheters-in-infants-and-young-children
#1
Amanda Marie Marshall, David A Danford, Christopher L Curzon, Venus Anderson, Jeffrey W Delaney
OBJECTIVES: Children with congenital heart disease may require long-term central venous access for intensive care management; however, central venous access must also be preserved for future surgical and catheterization procedures. Transhepatic venous catheters may be an useful alternative. The objective of this study was to compare transhepatic venous catheters with traditional central venous catheters regarding complication rate and duration of catheter service. DESIGN: Retrospective review of 12 congenital heart disease patients from September 2013 to July 2015 who underwent placement of one or more transhepatic venous catheters...
July 25, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28730304/novel-ribonuclease-activity-of-cusativin-from-cucumis-sativus-for-mapping-nucleoside-modifications-in-rna
#2
Balasubrahmanyam Addepalli, Sarah Venus, Priti Thakur, Patrick A Limbach
A recombinant ribonuclease, cusativin, was characterized for its cytidine-specific cleavage ability of RNA to map chemical modifications. Following purification of native cusativin protein as described before (Rojo et al. Planta 194:328, 17), partial amino acid sequencing was carried out to identify the corresponding protein coding gene in cucumber genome. Cloning and heterologous expression of the identified gene in Escherichia coli resulted in successful production of active protein as a C-terminal His-tag fusion protein...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28727249/dynamics-of-amino-acid-redistribution-in-the-carnivorous-venus-flytrap-dionaea-muscipula-after-digestion-of-13-c-15-n-labeled-prey
#3
Jörg Kruse, Peng Gao, Monika Eibelmeier, Saleh Alfarraj, Heinz Rennenberg
Amino acids represent an important component in the diet of the Venus flytrap (Dionaea muscipula), and supply plants with much needed nitrogen resources upon capture of insect prey. Little is known about the significance of prey-derived carbon backbones of amino acids for the success of Dionaea's carnivorous life-style. The present study tracked changes in plant amino acid pools and their δ(13) C- and δ(15) N-signatures, after half of all traps per plant had been fed with contrasting amounts of double-labeled insect powder (0, 5, 10 and 20 mg per trap)...
July 20, 2017: Plant Biology
https://www.readbyqxmd.com/read/28720939/cholesterol-analysis-of-korean-eat-out-foods-for-national-food-composition-database
#4
Farhana Sharmin, Beom-Gyun Jeong, Jiyoung Jung, Venus Quines, Jiyeon Chun
Information on cholesterol intake through restaurant meal is of high concern because of increasing eat-out population. Since nutrient labeling is not mandatory for restaurant food in Korea, cholesterol database on restaurant menu is unavailable. This study was performed to construct regional and national cholesterol database on primary Korean restaurant foods including 30 soup/stew, 24 rice dishes, and 27 noodles. From 2009 to 2012, Korea Ministry of Food and Drug Safety collected total 5832 foods (81 food types ×6 regions ×12 restaurants) nationwide and then 486 composites representing food types and regions were prepared for cholesterol analysis...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28713249/crispr-cas9-mediated-zebrafish-knock-in-as-a-novel-strategy-to-study-midbrain-hindbrain-boundary-development
#5
Gokul Kesavan, Avinash Chekuru, Anja Machate, Michael Brand
The midbrain-hindbrain boundary (MHB) acts as an organizer and controls the fate of neighboring cells to develop into either mesencephalic (midbrain) or metencephalic (hindbrain) cells by secreting signaling molecules like Wnt1 and Fgf8. The zebrafish is an excellent vertebrate model for studying MHB development due to the ease of gene manipulation and the possibility of following cellular dynamics and morphogenetic processes using live imaging. Currently, only very few reporter and/or Cre-driver lines are available to study gene expression at the MHB, hampering the understanding of MHB development, and traditional transgenic technologies using promoter/enhancer fragments or bacterial artificial chromosome (BAC)-mediated transgenesis often do not faithfully recapitulate endogenous expression patterns...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28701913/common-hepatic-branch-of-vagus-nerve-dependent-expression-of-immediate-early-genes-in-the-mouse-brain-by-intraportal-l-arginine-comparison-with-cholecystokinin-8
#6
Daisuke Yamada, Peter Koppensteiner, Saori Odagiri, Megumi Eguchi, Shun Yamaguchi, Tetsuya Yamada, Hideki Katagiri, Keiji Wada, Masayuki Sekiguchi
Information from the peripheral organs is thought to be transmitted to the brain by humoral factors and neurons such as afferent vagal or spinal nerves. The common hepatic branch of the vagus (CHBV) is one of the main vagus nerve branches, and consists of heterogeneous neuronal fibers that innervate multiple peripheral organs such as the bile duct, portal vein, paraganglia, and gastroduodenal tract. Although, previous studies suggested that the CHBV has a pivotal role in transmitting information on the status of the liver to the brain, the details of its central projections remain unknown...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28698356/root-bending-is-antagonistically-affected-by-hypoxia-and-erf-mediated-transcription-via-auxin-signaling
#7
Emese Eysholdt-Derzso, Margret Sauter
When plants encounter soil water logging or flooding, roots are the first organs to be confronted with reduced gas diffusion resulting in limited oxygen supply. Since roots do not generate photosynthetic oxygen they are rapidly faced with oxygen shortage rendering roots particularly prone to damage. While metabolic adaptations to low oxygen conditions, that ensure basic energy supply, have been well characterized, adaptation of root growth and development have received less attention. In this study we show that hypoxic conditions cause the primary root to grow sidewise in a low oxygen environment possibly to escape soil patches with reduced oxygen availability...
July 11, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28695960/galectin-1-enhances-the-generation-of-neural-crest-cells
#8
Tsutomu Motohashi, Masahiro Nishioka, Daisuke Kitagawa, Norito Kawamura, Natsuki Watanabe, Takanori Wakaoka, Toshihiko Kadoya, Takahiro Kunisada
Neural crest (NC) cells are multipotent cells that emerge from the dorsal region of the neural tube. After delaminating from the neural tube, NC cells migrate throughout the developing embryo and differentiate into various cells: neurons and glial cells of the peripheral nervous system, melanocytes of skin, and skeletal elements of the face and head. We previously analyzed the gene expression profile of a NC subpopulation isolated from Sox10-IRES-Venus mice and found that the carbohydrate-binding protein, Galectin-1 (Gal-1) was strongly expressed in generating NC cells...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28681555/the-structure-of-plasmodium-falciparum-3d7_0606800-reveals-a-bi-lobed-architecture-that-supports-re-annotation-as-a-venus-flytrap-protein
#9
Michelle L Parker, Raghavendran Ramaswamy, Kyle van Gordon, Cameron Powell, Jürgen Bosch, Martin J Boulanger
Plasmodium falciparum, the causative agent of malaria, employs a diverse array of surface displayed proteins to promote dissemination and establish infection in the human host. Of these, Pf3D7_0606800 is highly immunogenic and has been designated a potential top 10 candidate for inclusion in a multicomponent malarial vaccine. The role of Pf3D7_0606800 in parasite biology, however, is unknown and its characterization has been complicated by a lack of sequence identity with proteins of known structure or function...
July 6, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28676549/venus
#10
Abi Berger
No abstract text is available yet for this article.
July 2017: Journal of Family Planning and Reproductive Health Care
https://www.readbyqxmd.com/read/28675840/biosurfactant-production-by-aureobasidium-pullulans-in-stirred-tank-bioreactor-new-approach-to-understand-the-influence-of-important-variables-in-the-process
#11
Larissa Pereira Brumano, Felipe Antonio Fernandes Antunes, Sara Galeno Souto, Júlio Cesar Dos Santos, Joachim Venus, Roland Schneider, Silvio Silvério da Silva
Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, biotechnological process, aimed to develop new sustainable process configurations by using favorable microorganisms, already requires investigations in more details. Thus, we present a novel approach for biosurfactant production using the promising yeast Aureobasidium pullulans LB 83, in stirred tank reactor. A central composite face-centered design was carried out to evaluate the effect of the aeration rate (0...
June 19, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28642778/characterization-of-salt-induced-epigenetic-segregation-by-genome-wide-loss-of-heterozygosity-and-its-association-with-salt-tolerance-in-rice-oryza-sativa-l
#12
Min Li, Wen-Sheng Wang, Yun-Long Pang, Jessica R Domingo, Jauhar Ali, Jian-Long Xu, Bin-Ying Fu, Elec B Venus, Zhi-Kang Li
In a breeding effort to develop salt tolerant (ST) rice varieties by designed QTL pyramiding, large numbers of progenies derived from four crosses between salt- or drought- tolerant BC2F5 IR64 introgression lines, were subjected to severe salt stress, resulting in 422 ST plants. The progeny testing of the selected F3 lines under more severe salt stress resulted in identification of 16 promising homozygous lines with high levels of ST. Genetic characterization of the 422 ST F3 progeny and 318 random F2 plants from the same four crosses using 105 segregating SSR markers lead to three interesting discoveries: (1) salt stress can induce genome-wide epigenetic segregation (ES) characterized by complete loss of heterozygosity (LOH) and nearly complete loss of an allele (LOA) in the F3 progenies of four rice populations in a single generation; (2) ∼25% of the stress-induced ES loci were transgenerational and inherited from their salt- and drought- selected parents; and (3) the salt-induced LOH and LOA loci (regions) appeared to contain genes/alleles associated with ST and/or drought tolerance...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28637823/titin-force-enhancement-following-active-stretch-of-skinned-skeletal-muscle-fibres
#13
Krysta Powers, Venus Joumaa, Azim Jinha, Eng Kuan Moo, Ian Curtis Smith, Kiisa Nishikawa, Walter Herzog
In actively stretched skeletal muscle sarcomeres, titin-based force is enhanced, increasing the stiffness of active sarcomeres. Titin force enhancement in sarcomeres is vastly reduced in mdm, a genetic mutation with a deletion in titin. Whether loss of titin force enhancement is associated with compensatory mechanisms at higher structural levels of organization, such as single fibres or entire muscles, is unclear. The aim of this study was to determine whether mechanical deficiencies in titin force enhancement are also observed at the fibre level, and whether mechanisms compensate for the loss of titin force enhancement...
June 21, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28634254/widespread-paleopolyploidy-gene-tree-conflict-and-recalcitrant-relationships-among-the-carnivorous-caryophyllales
#14
Joseph F Walker, Ya Yang, Michael J Moore, Jessica Mikenas, Alfonso Timoneda, Samuel F Brockington, Stephen A Smith
PREMISE OF STUDY: The carnivorous members of the large, hyperdiverse Caryophyllales (e.g., Venus flytrap, sundews, and Nepenthes pitcher plants) represent perhaps the oldest and most diverse lineage of carnivorous plants. However, despite numerous studies seeking to elucidate their evolutionary relationships, the early-diverging relationships remain unresolved. METHODS: To explore the utility of phylogenomic data sets for resolving relationships among the carnivorous Caryophyllales, we sequenced 10 transcriptomes, including all the carnivorous genera except those in the rare West African liana family Dioncophyllaceae...
June 20, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28628735/effect-of-shade-opacity-and-layer-thickness-on-light-transmission-through-a-nano-hybrid-dental-composite-during-curing
#15
Hong-Keun Hyun, Carly K Christoferson, Carmem S Pfeifer, Chris Felix, Jack L Ferracane
OBJECTIVE: The aim of this study was to investigate the effect of shade and opacity on the change in light transmission through different thicknesses of a nano-hybrid composite during curing. MATERIALS AND METHODS: Twelve different shades of Venus Diamond (Heraeus Kulzer) were placed in disk shaped molds with thickness of 1, 2, and 3 mm (n = 3 per group) and cured with an LED light-curing unit. Initial, final and average irradiance, and the total amount of energy passing through the specimen were measured using the MARC Resin Calibrator at every 10s for a total of 40s...
June 19, 2017: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/28626203/rheological-approach-for-determining-yield-stresses-in-flowable-resin-composites-prior-to-setting
#16
Masaki Asakura, Tatsuhide Hayashi, Shozo Tsuruta, Mitsunori Yamamoto, Shuichiro Kobayashi, Akimichi Mieki, Tatsushi Kawai
The purpose of this study is to develop a method for quantifying the fluidity of flowable resin composites using determinations of yield stress. Five commercially available composites (AliteFlo LV, Flow-it ALC, Venus flow, Tetric N-flow, Revolution Formula2) were investigated. Yield stress values were obtained by plotting shear stresses for a range of shear rates, followed by fitting of Casson fluid models to flow curve data and extrapolation to the stress axis. To confirm that yield stress reflected fluidity, apparent viscosity at the lower shear rate (0...
June 15, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28601899/sensory-innervation-of-the-anal-canal-and-anorectal-line-in-hirschsprung-s-disease-histological-evidence-from-mouse-models
#17
Masahiro Takeda, Katsumi Miyahara, Chihiro Akazawa, Geoffrey J Lane, Atsuyuki Yamataka
AIM: We used non-Hirschsprung's disease (HD) Sox10-Venus Transgenic mice (non-HDSV-mice), an endothelin receptor-B knockout mouse model of HD (HD-mice), and C57B6C3 wild controls (C-mice) to identify the correlation between the anorectal line (ARL) and successful transanal pull-through (TAPT). METHODS: In non-HDSV-mice, intestinal neural crest-derived cells can be visualized with Venus,-a green fluorescent protein-without histochemical staining. We exposed the anal canal in each non-HDSV-mouse and marked the ARL directly with red ink...
August 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28600944/investigation-of-food-waste-valorization-through-sequential-lactic-acid-fermentative-production-and-anaerobic-digestion-of-fermentation-residues
#18
Francesca Demichelis, Daniel Pleissner, Silvia Fiore, Silvia Mariano, Ivette Michelle Navarro Gutiérrez, Roland Schneider, Joachim Venus
This work concerns the investigation of the sequential production of lactic acid (LA) and biogas from food waste (FW). LA was produced from FW using a Streptococcus sp. strain via simultaneous saccharification and fermentation (SSF) and separate enzymatic hydrolysis and fermentation (SHF). Via SHF a yield of 0.33gLA/gFW (productivity 3.38gLA/L·h) and via SSF 0.29gLA/gFW (productivity 2.08gLA/L·h) was obtained. Fermentation residues and FW underwent anaerobic digestion (3wt% TS). Biogas yields were 0.71, 0...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28600331/does-maternal-hsv-2-coinfection-increase-mother-to-child-transmission-of-hiv-a-systematic-review
#19
REVIEW
Vishalini Sivarajah, Kevin Venus, Mark H Yudin, Kellie E Murphy, Steven A Morrison, Darrell Hs Tan
BACKGROUND: Reducing HIV mother-to-child transmission (MTCT) is critical to ending the HIV pandemic. Reports suggest that herpes simplex virus-2 (HSV-2), a common coinfection in HIV-infected individuals, is associated with increased MTCT, but results have been conflicting. We conducted a systematic review of observational studies to quantify the magnitude of this relationship (PROSPERO no. CRD42016043315). METHODS: We searched Medline (1981 to June week 3, 2016), EMBASE (1981 to week 26, 2016), relevant conferences (2013-2016) and bibliographies of identified studies for cohort and case-control studies enrolling HIV-positive women during pregnancy or peripartum that quantified the effect of HSV-2 infection on MTCT...
June 9, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28580283/foxa2-and-pdx1-cooperatively-regulate-postnatal-maturation-of-pancreatic-%C3%AE-cells
#20
Aimée Bastidas-Ponce, Sara S Roscioni, Ingo Burtscher, Erik Bader, Michael Sterr, Mostafa Bakhti, Heiko Lickert
OBJECTIVE: The transcription factors (TF) Foxa2 and Pdx1 are key regulators of beta-cell (β-cell) development and function. Mutations of these TFs or their respective cis-regulatory consensus binding sites have been linked to maturity diabetes of the young (MODY), pancreas agenesis, or diabetes susceptibility in human. Although Foxa2 has been shown to directly regulate Pdx1 expression during mouse embryonic development, the impact of this gene regulatory interaction on postnatal β-cell maturation remains obscure...
June 2017: Molecular Metabolism
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