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https://www.readbyqxmd.com/read/28207991/high-yield-secretion-of-recombinant-proteins-from-the-microalga-chlamydomonas-reinhardtii
#1
E M Ramos-Martinez, L Fimognari, Y Sakuragi
Microalga-based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability, and sustainability. Secretion of recombinant proteins can accelerate the use of microalgal platforms by allowing post-translational modifications and easy recovery of products from the culture media. However, currently, the yields of secreted recombinant proteins are low, which hampers the commercial application of this strategy. This study aimed at expanding the genetic tools for enhancing secretion of recombinant proteins in Chlamydomonas reinhardtii, a widely used green microalga as a model organism and a potential industrial biotechnology platform...
February 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28188935/model-based-characterisation-of-growth-performance-and-l-lactic-acid-production-with-high-optical-purity-by-thermophilic-bacillus-coagulans-in-a-lignin-supplemented-mixed-substrate-medium
#2
Robert Glaser, Joachim Venus
Three Bacillus coagulans strains were characterised in terms of their ability to grow in lignin-containing fermentation media and to consume the lignocellulose-related sugars glucose, xylose, and arabinose. An optical-density high-throughput screening was used for precharacterisation by means of different mathematical models for comparison (Logistic, Gompertz, Baranyi, Richards & Stannard, and Schnute). The growth response was characterised by the maximum growth rate and lag time. For a comparison of the screening and fermentation results, an unstructured mathematical model was proposed to characterise the lactate production, bacterial growth and substrate consumption...
February 7, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28179551/olecranon-bursitis-beau-s-lines-biett-s-collarettes-and-crown-of-venus
#3
Walter de Araujo Eyer-Silva, Guilherme Almeida Rosa da Silva, Fernando Raphael de Almeida Ferry
No abstract text is available yet for this article.
February 8, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28149461/microbiological-characterization-and-effect-of-resin-composites-in-cervical-lesions
#4
Bonfanti Carlo, Nicola Barabanti, Giorgio Piccinelli, Vicente Faus-Matoses, Antonio Cerutti
BACKGROUND: Non carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect, in vivo and in vitro, against S. mutans. MATERIAL AND METHODS: Eight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28133595/investigation-of-spiral-wound-membrane-modules-for-the-cross-flow-nanofiltration-of-fermentation-broth-obtained-from-a-pilot-plant-fermentation-reactor-for-the-continuous-production-of-lactic-acid
#5
Hendrik Laube, Roland Schneider, Joachim Venus
BACKGROUND: The separation performance of seven polymer membranes for the nanofiltration of sodium lactate in fermentation broth was investigated. Each module was introduced into the test stand, and the system curve was obtained by recording the permeate flow velocity at different pump head levels. Performance benchmarks were good permeate quality, as determined by high permeate flow velocity, high sodium lactic concentration, low ion impurity concentration, and low organic impurity concentration...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28114363/evidence-for-integrin-venus-kinase-receptor-1-alliance-in-the-ovary-of-schistosoma-mansoni-females-controlling-cell-survival
#6
Verena Gelmedin, Marion Morel, Steffen Hahnel, Katia Cailliau, Colette Dissous, Christoph G Grevelding
In metazoan integrin signaling is an important process of mediating extracellular and intracellular communication processes. This can be achieved by cooperation of integrins with growth factor receptors (GFRs). Schistosoma mansoni is a helminth parasite inducing schistosomiasis, an infectious disease of worldwide significance for humans and animals. First studies on schistosome integrins revealed their role in reproductive processes, being involved in spermatogenesis and oogenesis. With respect to the roles of eggs for maintaining the parasite´s life cycle and for inducing the pathology of schistosomiasis, elucidating reproductive processes is of high importance...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28096368/elemental-sulfur-aerosol-forming-mechanism
#7
Manoj Kumar, Joseph S Francisco
Elemental sulfur aerosols are ubiquitous in the atmospheres of Venus, ancient Earth, and Mars. There is now an evolving body of evidence suggesting that these aerosols have also played a role in the evolution of early life on Earth. However, the exact details of their formation mechanism remain an open question. The present theoretical calculations suggest a chemical mechanism that takes advantage of the interaction between sulfur oxides, SOn (n = 1, 2, 3) and hydrogen sulfide (nH2S), resulting in the efficient formation of a Sn+1 particle...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28073570/are-men-from-mars-and-women-from-venus
#8
EDITORIAL
George L Hicks
No abstract text is available yet for this article.
December 12, 2016: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28052100/on-the-effect-of-planetary-stable-isotope-compositions-on-growth-and-survival-of-terrestrial-organisms
#9
Xueshu Xie, Roman A Zubarev
Isotopic compositions of reactants affect the rates of chemical and biochemical reactions. Usually it is assumed that heavy stable isotope enrichment leads to progressively slower reactions. Yet the effect of stable isotopes may be nonlinear, as exemplified by the "isotopic resonance" phenomenon. Since the isotopic compositions of other planets of Solar system, including Mars and Venus, are markedly different from terrestrial (e.g., deuterium content is ≈5 and ≈100 times higher, respectively), it is far from certain that terrestrial life will thrive in these isotopic conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28042877/the-carnivorous-venus-flytrap-uses-prey-derived-amino-acid-carbon-to-fuel-respiration
#10
Lukas Fasbender, Daniel Maurer, Jürgen Kreuzwieser, Ines Kreuzer, Waltraud X Schulze, Jörg Kruse, Dirk Becker, Saleh Alfarraj, Rainer Hedrich, Christiane Werner, Heinz Rennenberg
The present study was performed to elucidate the fate of carbon (C) and nitrogen (N) derived from protein of prey caught by carnivorous Dionaea muscipula. For this, traps were fed (13) C/(15) N-glutamine (Gln). The release of (13) CO2 was continuously monitored by isotope ratio infrared spectrometry. After 46 h, the allocation of C and N label into different organs was determined and tissues were subjected to metabolome, proteome and transcriptome analyses. Nitrogen of Gln fed was already separated from its C skeleton in the decomposing fluid secreted by the traps...
January 2, 2017: New Phytologist
https://www.readbyqxmd.com/read/28035654/seven-days-post-injury-fate-and-effects-of-genetically-labelled-adipose-derived-mesenchymal-cells-on-a-rat-traumatic-brain-injury-experimental-model
#11
Ioanna Dori, Spyros Petrakis, Aggeliki Giannakopoulou, Chryssa Bekiari, Ioannis Grivas, Evangelia K Siska, Georgios Koliakos, Georgios C Papadopoulos
Mesenchymal stromal cells (MSC) have been suggested to have beneficial effects on animal models of traumatic brain injury (TBI), owing to their neurotrophic and immunomodulatory properties. Adipose tissue-derived stromal cells (ASCs) are multipotent MSC that can be harvested with minimally invasive methods, show a high proliferative capacity, low immunogenicity if allogeneic, and can be used in autologous or heterologous settings. In the present study ASCs were genetically labelled using the Sleeping Beauty transposon to express the fluorescent protein Venus...
December 30, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/28034957/a-three-dimensional-parf-meshwork-assembles-through-the-nucleoid-to-mediate-plasmid-segregation
#12
Brett N McLeod, Gina E Allison-Gamble, Madhuri T Barge, Nam K Tonthat, Maria A Schumacher, Finbarr Hayes, Daniela Barillà
Genome segregation is a fundamental step in the life cycle of every cell. Most bacteria rely on dedicated DNA partition proteins to actively segregate chromosomes and low copy-number plasmids. Here, by employing super resolution microscopy, we establish that the ParF DNA partition protein of the ParA family assembles into a three-dimensional meshwork that uses the nucleoid as a scaffold and periodically shuttles between its poles. Whereas ParF specifies the territory for plasmid trafficking, the ParG partner protein dictates the tempo of ParF assembly cycles and plasmid segregation events by stimulating ParF adenosine triphosphate hydrolysis...
December 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/28024626/self-assembly-of-aromatic-%C3%AE-amino-acids-into-amyloid-inspired-nano-micro-scaled-architects
#13
Prabhjot Singh, Surinder K Brar, Manish Bajaj, Nikesh Narang, Venus S Mithu, Om P Katare, Nishima Wangoo, Rohit K Sharma
In the pursuit for design of novel bio inspired materials, aromatic α-amino acids (phenylalanine, tyrosine, tryptophan and histidine) have been investigated for the generation of well-ordered self-assembled architects such as fibrils, rods, ribbons and twisted nanosheets in varying solvent systems. These nano/micro scaled architects were thoroughly characterized using FE-SEM, confocal microscopy, optical microscopy, (1)H NMR, FTIR, XRD and TGA. These self-assembled architects were histologically stained with Congo red and thioflavin T dyes for investigation of amyloid morphology which revealed that the deposited state of ordered assemblies exhibit specific characteristic of amyloid deposits...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28013333/semaphorin-3a-expression-following-intestinal-ischemia-reperfusion-injury-in-sox10-venus-mice
#14
Masahiro Takeda, Katsumi Miyahara, Manabu Okawada, Chihiro Akazawa, Geoffrey J Lane, Atsuyuki Yamataka
PURPOSE: Semaphorin 3A (Sema3A) is a protein secreted during development of the nervous system that plays an important role in neuronal pathophysiology. However, there is no known correlation between Sema3A and intestinal ischemia/reperfusion (I/R) injury. We assessed Sema3A expression and distribution in relation to enteric nervous system (ENS) damage seen after intestinal I/R injury in Sox10-Venus mice. METHODS: Intestinal I/R injury was induced by vascular occlusion for 3 h...
December 24, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/28012897/visualization-of-herpes-simplex-virus-type-1-virions-using-fluorescent-colors
#15
Lyns Etienne, Poorval Joshi, Laura Dingle, Eugene Huang, Peter Grzesik, Prashant J Desai
Our laboratory was one of the first to engineer a live fluorescent tag, enhanced green fluorescent protein (eGFP), that marked the capsid of herpes simplex virus type 1 (HSV-1) and subsequently maturing virus as the particle made its way to the cell surface. In the present study we sought to increase the repertoire of colors available as fusion to the small capsid protein, VP26, so that they can be used alone or in conjunction with other fluorescent tags (fused to other HSV proteins) to follow the virus as it enters and replicates within the cell...
March 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28007831/venus
#16
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Journal of Family Planning and Reproductive Health Care
https://www.readbyqxmd.com/read/27995902/plant-inspired-adaptive-structures-and-materials-for-morphing-and-actuation-a-review
#17
Suyi Li, K W Wang
Plants exhibit a variety of reversible motions, from the slow opening of pine cones to the impulsive closing of Venus flytrap leaves. These motions are achieved without muscles and they have inspired a wide spectrum of engineered materials and structures. This review summarizes the recent developments of plant-inspired adaptive structures and materials for morphing and actuation. We begin with a brief overview of the actuation strategies and physiological features associated to these plant movements, showing that different combinations of these strategies and features can lead to motions with different deformation characteristics and response speeds...
December 20, 2016: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/27981221/successful-use-of-secukinumab-in-pustular-psoriasis
#18
Jensen Yeung, Venus Valbuena
No abstract text is available yet for this article.
November 2016: JAAD Case Reports
https://www.readbyqxmd.com/read/27943188/purification-of-synaptosome-populations-using-fluorescence-activated-synaptosome-sorting
#19
Elisa Luquet, Christoph Biesemann, Annie Munier, Etienne Herzog
For several decades, neurobiologists have used subcellular fractionation methods to analyze the molecular structure and some functional features of the cells in the central nervous system. Indeed, brain tissue contains a complex intermingled network of neuronal, glial, and vascular cells. To reduce this complexity biochemists have optimized fractionation protocols that enrich in specific compartments such as synapses (called "synaptosomes") and synaptic vesicles, for example. However, recently, these approaches suffered from a lack of specificity and purity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27943178/early-experience-with-the-venus-p%C3%A2-valve-for-percutaneous-pulmonary-valve-implantation-in-native-outflow-tract
#20
F Garay, X Pan, Y J Zhang, C Wang, D Springmuller
INTRODUCTION: The Venus p‑valve (MedTech, Shanghai, China) is a self-expanding percutaneous heart valve designed to be implanted in a native patched right ventricle outflow tract. The worldwide clinical experience with this valve is just beginning and the results have so far been encouraging. We present our initial early experience implanting the Venus p‑valve in the native right ventricle outflow tract of patients with Tetralogy of Fallot repaired with a transannular patch. METHODS: In 10 selected patients a procedure for percutaneous pulmonary valve implantation was performed using the Venus p‑valve...
February 2017: Netherlands Heart Journal
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