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https://www.readbyqxmd.com/read/28926625/defining-the-molecular-basis-of-interaction-between-r3-receptor-type-protein-tyrosine-phosphatases-and-ve-cadherin
#1
Olga Dorofejeva, Alastair J Barr
Receptor-type protein tyrosine phosphatases (RPTPs) of the R3 subgroup play key roles in the immune, vascular and nervous systems. They are characterised by a large ectodomain comprising multiple FNIII-like repeats, a transmembrane domain, and a single intracellular phosphatase domain. The functional role of the extracellular region has not been clearly defined and potential roles in ligand interaction, dimerization, and regulation of cell-cell contacts have been reported. Here bimolecular fluorescence complementation (BiFC) in live cells was used to examine the molecular basis for the interaction of VE-PTP with VE-cadherin, two proteins involved in endothelial cell contact and maintenance of vascular integrity...
2017: PloS One
https://www.readbyqxmd.com/read/28916758/role-of-the-venus-kinase-receptor-in-the-female-reproductive-physiology-of-the-desert-locust-schistocerca-gregaria
#2
Cynthia Lenaerts, Jolien Palmans, Elisabeth Marchal, Rik Verdonck, Jozef Vanden Broeck
Venus kinase receptors (VKR) are a subfamily of invertebrate receptor tyrosine kinases, which have only recently been discovered. They contain an intracellular tyrosine kinase domain and an extracellular Venus FlyTrap domain. VKRs have been functionally and pharmacologically characterized in only two invertebrate species, namely the human parasite Schistosoma mansoni and the mosquito Aedes aegypti, where they play a crucial role in oogenesis. Here, we report the characterization of a VKR in the desert locust, Schistocerca gregaria...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28883436/pandemic-2009-h1n1-influenza-venus-reporter-virus-reveals-broad-diversity-of-mhc-class-ii-positive-antigen-bearing-cells-following-infection-in-vivo
#3
Anthony DiPiazza, Aitor Nogales, Nicholas Poulton, Patrick C Wilson, Luis Martínez-Sobrido, Andrea J Sant
Although it is well established that Influenza A virus infection is initiated in the respiratory tract, the sequence of events and the cell types that become infected or access viral antigens remains incompletely understood. In this report, we used a novel Influenza A/California/04/09 (H1N1) reporter virus that stably expresses the Venus fluorescent protein to identify antigen-bearing cells over time in a mouse model of infection using flow cytometry. These studies revealed that many hematopoietic cells, including subsets of monocytes, macrophages, dendritic cells, neutrophils and eosinophils acquire influenza antigen in the lungs early post-infection...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28877671/surveillance-of-device-associated-infections-and-mortality-in-a-major-intensive-care-unit-in-the-republic-of-cyprus
#4
Stelios Iordanou, Nicos Middleton, Elizabeth Papathanassoglou, Vasilios Raftopoulos
BACKGROUND: Device-associated health care-associated infections (DA-HAI) pose a threat to patient safety, particularly in the intensive care unit. The aim of this study was to assess the incidence of DA-HAIs, mortality and crude excess mortality at a General Hospital's Intensive Care Unit (ICU) in the Republic of Cyprus for 1 year period. METHODS: A prospective cohort, active DA-HAIs surveillance study with the use of Health Acquired Infections (HAIs) ICU Protocol (v1...
September 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28870988/a-fluorescent-toolkit-for-spatiotemporal-tracking-of-apoptotic-cells-in-living-drosophila-tissues
#5
Sonia Schott, Arnaud Ambrosini, Audrey Barbaste, Corinne Benassayag, Mélanie Gracia, Amsha Proag, Mégane Rayer, Bruno Monier, Magali Suzanne
Far from being passive, apoptotic cells influence their environment. For instance, they promote tissue folding, myoblast fusion and modulate tumor growth. Understanding the role of apoptotic cells necessitates their efficient tracking within living tissues, a task which is currently challenging. In order to easily spot apoptotic cells in developing Drosophila tissues, we generated a series of fly lines expressing different fluorescent sensors of caspase activity. We show that three of these reporters (GFP, Cerulean and Venus derived molecules) are detected specifically in apoptotic cells and throughout the whole process of programmed cell death...
September 4, 2017: Development
https://www.readbyqxmd.com/read/28860184/a-bimolecular-fluorescence-complementation-tool-for-identification-of-protein-protein-interactions-in-candida-albicans
#6
Ana Subotić, Erwin Swinnen, Liesbeth Demuyser, Herlinde De Keersmaecker, Hideaki Mizuno, Hélène Tournu, Patrick Van Dijck
Investigation of protein-protein interactions (PPI) in Candida albicans is essential for understanding the regulation of the signal transduction network that triggers its pathogenic lifestyle. Unique features of C. albicans, such as the alternative codon usage and incomplete meiosis, have enforced the optimization of standard genetic methods as well as development of novel approaches. Since the existing methods for detection of PPI are limited for direct visualization of the interacting complex in vivo, we have established a bimolecular fluorescence complementation (BiFC) in C...
August 31, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28857590/an-empirical-study-of-multidimensional-fidelity-of-compass-consultation
#7
Venus Wong, Lisa A Ruble, John H McGrew, Yue Yu
Consultation is essential to the daily practice of school psychologists (National Association of School Psychologist, 2010). Successful consultation requires fidelity at both the consultant (implementation) and consultee (intervention) levels. We applied a multidimensional, multilevel conception of fidelity (Dunst, Trivette, & Raab, 2013) to a consultative intervention called the Collaborative Model for Promoting Competence and Success (COMPASS) for students with autism. The study provided 3 main findings. First, multidimensional, multilevel fidelity is a stable construct and increases over time with consultation support...
August 31, 2017: School Psychology Quarterly
https://www.readbyqxmd.com/read/28839201/development-of-an-ensemble-kalman-filter-data-assimilation-system-for-the-venusian-atmosphere
#8
Norihiko Sugimoto, Akira Yamazaki, Toru Kouyama, Hiroki Kashimura, Takeshi Enomoto, Masahiro Takagi
The size and mass of Venus is similar to those of the Earth; however, its atmospheric dynamics are considerably different and they are poorly understood due to limited observations and computational difficulties. Here, we developed a data assimilation system based on the local ensemble transform Kalman filter (LETKF) for a Venusian Atmospheric GCM for the Earth Simulator (VAFES), to make full use of the observational data. To examine the validity of the system, two datasets were assimilated separately into the VAFES forecasts forced with solar heating that excludes the diurnal component Qz; one was created from a VAFES run forced with solar heating that includes the diurnal component Qt, whereas the other was based on observations made by the Venus Monitoring Camera (VMC) onboard the Venus Express...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28831235/benign-heart-murmurs-as-a-predictor-for-complications-following-total-joint-arthroplasty
#9
James M Friedman, Ricardo Couso, Michael Kitchens, Venus Vakhshori, Cody D Hillin, Chia H Wu, Joshua Steere, Jaimo Ahn, Eric Hume
BACKGROUND: There is scant literature examining the predictive role of heart murmurs in the absence of suspected structural heart disease on complications of non-cardiac surgery. We hypothesize the detection of heart murmurs in the absence of structural heart disease will help identify patients at risk for complications following total joint arthroplasty (TJA) surgery. METHOD: This was a prospective cohort of patients undergoing TJA over a twenty-month period. The study was performed at a single academic institution with four subspecialty surgeons...
December 2017: Journal of Orthopaedics
https://www.readbyqxmd.com/read/28816457/a-coupled-cluster-investigation-of-sno-radical-isomers-and-their-reactions-with-hydrogen-atom-insight-into-structures-conformers-barriers-and-energetics
#10
Manoj Kumar, Joseph S Francisco
High-level coupled cluster theory with single and double excitation, and including a perturbative triples correction (CCSD(T)) method and a series of Dunning's augmented correlation consistent basis sets, aug-cc-pVXZ (X = D, T, Q, and 5) was applied to examine the conformational landscape of SNO radical system. The basis set has an important effect on the relative stability of SNO radical isomers; that is, at the CCSD(T)/aug-cc-pV5Z level of theory, the NSO radical is the most stable member of SNO radical family...
August 25, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28812643/a-submarine-volcanic-eruption-leads-to-a-novel-microbial-habitat
#11
Roberto Danovaro, Miquel Canals, Michael Tangherlini, Antonio Dell'Anno, Cristina Gambi, Galderic Lastras, David Amblas, Anna Sanchez-Vidal, Jaime Frigola, Antoni M Calafat, Rut Pedrosa-Pàmies, Jesus Rivera, Xavier Rayo, Cinzia Corinaldesi
Submarine volcanic eruptions are major catastrophic events that allow investigation of the colonization mechanisms of newly formed seabed. We explored the seafloor after the eruption of the Tagoro submarine volcano off El Hierro Island, Canary Archipelago. Near the summit of the volcanic cone, at about 130 m depth, we found massive mats of long, white filaments that we named Venus's hair. Microscopic and molecular analyses revealed that these filaments are made of bacterial trichomes enveloped within a sheath and colonized by epibiotic bacteria...
April 24, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28811218/wnt-signaling-positively-regulates-endothelial-cell-fate-specification-in-the-fli1a-positive-progenitor-population-via-lef1
#12
Kathleen Hübner, Kathrin S Grassme, Jyoti Rao, Nina K Wenke, Cordula L Zimmer, Laura Korte, Katja Mu Ller, Saulius Sumanas, Boris Greber, Wiebke Herzog
During vertebrate embryogenesis, vascular endothelial cells (ECs) and primitive erythrocytes become specified within close proximity in the posterior lateral plate mesoderm (LPM) from a common progenitor. However, the signaling cascades regulating the specification into either lineage remain largely elusive. Here, we analyze the contribution of β-catenin dependent Wnt signaling to EC and erythrocyte specification during zebrafish embryogenesis. We generated novel β-catenin dependent Wnt signaling reporters which, by using destabilized fluorophores (Venus-Pest, dGFP), specifically allow us to detect Wnt signaling responses in narrow time windows as well as in spatially restricted domains, defined by Cre recombinase expression (Tg(axin2BAC:Venus-Pest)(mu288); Tg(14TCF:loxP-STOP-loxP-dGFP)(mu202))...
August 12, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28810409/celebrity-endorsed-e-cigarette-brand-instagram-advertisements-effects-on-young-adults-attitudes-towards-e-cigarettes-and-smoking-intentions
#13
Joe Phua, Seunga Venus Jin, Jung Min Hahm
Celebrities endorsing e-cigarette brands on social media may exert a strong influence on e-cigarette uptake. Using a between-subject experiment, this study examines the effects of endorser type (celebrities, non-celebrities and products only) in e-cigarette brand Instagram advertisements on e-cigarette attitudes and smoking intentions. Results showed that celebrity endorsers significantly increased positive attitudes towards e-cigarettes and smoking intentions, compared to non-celebrities or products only. Celebrity endorsers also rated significantly higher on trustworthiness, expertise, goodwill and attractiveness, compared to non-celebrities...
February 1, 2017: Journal of Health Psychology
https://www.readbyqxmd.com/read/28782999/environmental-factors-affecting-escherichia-coli-concentrations-in-striped-venus-clam-chamelea-gallina-l-harvested-in-the-north-adriatic-sea
#14
Giulia Tabanelli, Chiara Montanari, Aldo Gardini, Mirko Maffei, Chiara Prioli, Fausto Gardini
The aim of this work was to evaluate the microbiological quality of striped venus clams (Chamelea gallina L.) harvested in the north Adriatic Sea during an 8-year monitoring period. A total of 387 samples were analyzed to assess the presence of Escherichia coli. Environmental parameters (salinity, pH, dissolved oxygen, seawater temperature, and freshwater outflow) were collected to find out a possible relationship between the E. coli counts and environmental factors. The results evidenced that the microbiological quality of the clams was good, with only about 5% of the samples not complying with European and Italian regulations for this product, that is, with E...
September 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28782541/investigating-the-efficacy-of-web-based-transfer-training-on-independent-wheelchair-transfers-through-randomized-control-trials
#15
Lynn Worobey, Stephanie Rigot, Nathan Hogaboom, Chris Venus, Michael L Boninger
OBJECTIVES: To determine the efficacy of a web-based transfer training module at improving transfer technique across three groups: web-based training, in-person training (current standard of practice), and a waitlist control group (WLCG). Secondarily, to determine subject factors that can be used to predict improvements in transfer ability after training. DESIGN: Randomized control trials. SETTING: Summer and winter sporting events for disabled veterans...
August 3, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28780442/cold-plasma-interactions-with-plants-morphing-and-movements-of-venus-flytrap-and-mimosa-pudica-induced-by-argon-plasma-jet
#16
Alexander G Volkov, Kunning G Xu, Vladimir I Kolobov
Low temperature (cold) plasma finds an increasing number of applications in biology, medicine and agriculture. In this paper, we report a new effect of plasma induced morphing and movements of Venus flytrap and Mimosa pudica. We have experimentally observed plasma activation of sensitive plant movements and morphing structures in these plants similar to stimulation of their mechanosensors in vivo. Application of an atmospheric pressure argon plasma jet to the inside or outside of a lobe, midrib, or cilia in Dionaea muscipula Ellis induces trap closing...
July 29, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28767146/a-randomized-trial-of-effects-of-alcohol-on-cytochrome-p450-eicosanoids-mediators-of-inflammation-resolution-and-blood-pressure-in-men
#17
Anne E Barden, Venus Chavez, Michael Phillips, Emilie Mas, Lawrence J Beilin, Kevin D Croft, Trevor A Mori, Ian B Puddey
BACKGROUND: Cardiovascular effects of alcohol consumption may be influenced by both pro- and anti-inflammatory mechanisms. We previously showed that chronic alcohol consumption increased blood pressure (BP), oxidative stress, and 20-hydroxyeicosatetraenoic acid (20-HETE), a vasoconstrictor and pro-inflammatory eicosanoid synthesized by cytochrome P450 (CYP450) enzymes from arachidonic acid. This study in men examined the effect of consuming red wine (RW) on BP in relation to changes in 20-HETE, oxidative stress (F2 -isoprostanes), markers of inflammation, anti-inflammatory CYP450 epoxyeicosatrienoic acids (EETs), and specialized pro-resolving mediators of inflammation (SPMs) derived from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)...
August 2, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28752830/healthcare-associated-infection-good-practices-knowledge-and-the-locus-of-control-in-heatlhcare-professionals
#18
Chiara Taffurelli, Alfonso Sollami, Carmen Camera, Francesca Federa, Annise Grandi, Marcella Marino, Tiziano Marrosu, Leopoldo Sarli
BACKGROUND AND AIM:   The incidence of Healthcare Associated Infections (HAI) is an important indicator of the quality of care. The behaviors associated with the prevention of infections are not only supported by rational knowledge or motivation, but are mediated by social, emotional and often stereotyped behaviors. The awarness of the good practices related to HAI, may be a factor. Other studies, identify how the perception of the problem in healthcare professionals is often influenced by a tendency towards an external Locus of Control: the patient, the family, the other wards, other care settings...
July 18, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/28746169/traditional-long-term-central-venous-catheters-versus-transhepatic-venous-catheters-in-infants-and-young-children
#19
Amanda Marie Marshall, David A Danford, Christopher L Curzon, Venus Anderson, Jeffrey W Delaney
OBJECTIVES: Children with congenital heart disease may require long-term central venous access for intensive care management; however, central venous access must also be preserved for future surgical and catheterization procedures. Transhepatic venous catheters may be an useful alternative. The objective of this study was to compare transhepatic venous catheters with traditional central venous catheters regarding complication rate and duration of catheter service. DESIGN: Retrospective review of 12 congenital heart disease patients from September 2013 to July 2015 who underwent placement of one or more transhepatic venous catheters...
July 25, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28730304/novel-ribonuclease-activity-of-cusativin-from-cucumis-sativus-for-mapping-nucleoside-modifications-in-rna
#20
Balasubrahmanyam Addepalli, Sarah Venus, Priti Thakur, Patrick A Limbach
A recombinant ribonuclease, cusativin, was characterized for its cytidine-specific cleavage ability of RNA to map chemical modifications. Following purification of native cusativin protein as described before (Rojo et al. Planta 194:328, 17), partial amino acid sequencing was carried out to identify the corresponding protein coding gene in cucumber genome. Cloning and heterologous expression of the identified gene in Escherichia coli resulted in successful production of active protein as a C-terminal His-tag fusion protein...
July 20, 2017: Analytical and Bioanalytical Chemistry
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