Read by QxMD icon Read


Chen Zhang, Cassandra L Miller, Rakshya Gorkhali, Juan Zou, Kenneth Huang, Edward M Brown, Jenny J Yang
Ca(2+)-sensing receptors (CaSRs) play a central role in regulating extracellular calcium concentration ([Ca(2+)]o) homeostasis and many (patho)physiological processes in multiple organs. This regulation is orchestrated by a cooperative response to extracellular stimuli such as small changes in Ca(2+), Mg(2+), amino acids, and other ligands. In addition, CaSR is a pleiotropic receptor regulating several intracellular signaling pathways, including calcium mobilization and intracellular calcium oscillation. Nearly 200 mutations and polymorphisms have been found in CaSR in relation to a variety of human disorders associated with abnormal Ca(2+) homeostasis...
2016: Frontiers in Physiology
Myriam A Badr, Jose R Pinto, Michael W Davidson, P Bryant Chase
Cardiac troponin C (cTnC) is a key effector in cardiac muscle excitation-contraction coupling as the Ca2+ sensing subunit responsible for controlling contraction. In this study, we generated several FRET sensors for divalent cations based on cTnC flanked by a donor fluorescent protein (CFP) and an acceptor fluorescent protein (YFP). The sensors report Ca2+ and Mg2+ binding, and relay global structural information about the structural relationship between cTnC's N- and C-domains. The sensors were first characterized using end point titrations to decipher the response to Ca2+ binding in the presence or absence of Mg2+...
2016: PloS One
Tobias Ackels, Daniela R Drose, Marc Spehr
In most mammals, the vomeronasal organ (VNO) is a chemosensory structure that detects both hetero- and conspecific social cues. Vomeronasal sensory neurons (VSNs) express a specific type of G protein-coupled receptor (GPCR) from at least three different chemoreceptor gene families allowing sensitive and specific detection of chemosensory cues. These families comprise the V1r and V2r gene families as well as the formyl peptide receptor (FPR)-related sequence (Fpr-rs) family of putative chemoreceptor genes. In order to understand the physiology of vomeronasal receptor-ligand interactions and downstream signaling, it is essential to identify the biophysical properties inherent to each specific class of VSNs...
2016: Journal of Visualized Experiments: JoVE
Naho Fujiwara, Katsumi Miyahara, Nana Nakazawa-Tanaka, Chihiro Akazawa, Atsuyuki Yamataka
PURPOSE: Hirschsprung's disease (HD) is caused by a failure of enteric neural crest-derived cells (ENCC) to colonize the bowel, resulting in an absence of the enteric nervous system (ENS). Previously, we developed a Sox10 transgenic version of the Endothelin receptor-B (Ednrb) mouse to visualize ENCC with the green fluorescent protein, Venus. The aim of this study was to isolate Sox10-Venus(+) cells, which are differentiated neurons and glial cells in the ENS, and analyze these cells using Sox10-Venus mice gut...
September 23, 2016: Pediatric Surgery International
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Anesthesiology
Xiao-Yu Chen, Zhi-Wei Zhu, Fu-Xian Yu, Jing Huang, Xiao-Rui Hu, Jian-Zhi Pan
Genomic integration of transgene by lentiviral vector has been proved an efficient method to produce single-transgenic animals. But it failed to create multi-gene transgenic offspring. Here, we have exploited lentivirus to generate the double-transgenic piglets through the female germline. The recombinant lentivirus containing fluorescent proteins genes (DsRed1 and Venus) were injected into the perivitelline space of 2-cell stage in vitro porcine embryos. Compared to control group, there was no significantly decreased in the proportion of blastocysts, and the two fluorescent protein genes were co-expressed in almost all the injected embryos...
June 28, 2016: Animal Reproduction Science
Junko Kono, Kohtarou Konno, Ashraf Hossain Talukder, Toshimitsu Fuse, Manabu Abe, Katsuya Uchida, Shuhei Horio, Kenji Sakimura, Masahiko Watanabe, Keiichi Itoi
We examined the morphological features of corticotropin-releasing factor (CRF) neurons in a mouse line in which modified yellow fluorescent protein (Venus) was expressed under the CRF promoter. We previously generated the CRF-Venus knock-in mouse, in which Venus is inserted into the CRF gene locus by homologous recombination. In the present study, the neomycin phosphotransferase gene (Neo), driven by the pgk-1 promoter, was deleted from the CRF-Venus mouse genome, and a CRF-Venus∆Neo mouse was generated. Venus expression is much more prominent in the CRF-Venus∆Neo mouse when compared to the CRF-Venus mouse...
September 16, 2016: Brain Structure & Function
Marion Morel, Mathieu Vanderstraete, Katia Cailliau, Steffen Hahnel, Christoph G Grevelding, Colette Dissous
Venus kinase receptors (VKRs) are invertebrate receptor tyrosine kinases (RTKs) formed by an extracellular Venus Fly Trap (VFT) ligand binding domain associated via a transmembrane domain with an intracellular tyrosine kinase (TK) domain. Schistosoma mansoni VKRs, SmVKR1 and SmVKR2, are both implicated in reproductive activities of the parasite. In this work, we show that the SH2 domain-containing protein SmShb is a partner of the phosphorylated form of SmVKR1. Expression of these proteins in Xenopus oocytes allowed us to demonstrate that the SH2 domain of SmShb interacts with the phosphotyrosine residue (pY979) located in the juxtamembrane region of SmVKR1...
2016: PloS One
Jumana Saleh
No abstract text is available yet for this article.
August 2016: Sultan Qaboos University Medical Journal
Martin Wiesholzer, Alexandra Winter, Manuel Kussmann, Markus Zeitlinger, Petra Pichler, Heinz Burgmann, Gottfried Reznicek, Wolfgang Poeppl
♦ Background: Intraperitoneal administration of antimicrobial agents is recommended for the treatment of peritoneal dialysis (PD)-related peritonitis. For home-based antimicrobial therapy it is common to supply patients with PD fluid bags with admixed antibiotic. Thus, the compatibility of meropenem with different PD fluids (PDFs), namely Extraneal, Physioneal 1.36% and Physioneal 2.27% (all Baxter Healthcare Corp., Deerfield, IL, USA), was investigated under varying storage conditions. ♦ Methods: Meropenem (Venus Pharma, Werne, Germany) was stored at 6°C and 25°C over 14 days and at 37°C over 24 hours...
September 7, 2016: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
T G Leighton, N Banda, B Berges, P F Joseph, P R White
The purpose of this project was to supply an acoustical simulation device to a local planetarium for use in live shows aimed at engaging and inspiring children in science and engineering. The device plays audio simulations of estimates of the sounds produced by natural phenomena to accompany audio-visual presentations and live shows about Venus, Mars, and Titan. Amongst the simulated noise are the sounds of thunder, wind, and cryo-volcanoes. The device can also modify the speech of the presenter (or audience member) in accordance with the underlying physics to reproduce those vocalizations as if they had been produced on the world under discussion...
August 2016: Journal of the Acoustical Society of America
Philippe Lognonné, Foivos Karakostas, Lucie Rolland, Yasuhiro Nishikawa
Acoustic coupling between solid Earth and atmosphere has been observed since the 1960s, first from ground-based seismic, pressure, and ionospheric sensors and since 20 years with various satellite measurements, including with global positioning system (GPS) satellites. This coupling leads to the excitation of the Rayleigh surface waves by local atmospheric sources such as large natural explosions from volcanoes, meteor atmospheric air-bursts, or artificial explosions. It contributes also in the continuous excitation of Rayleigh waves and associated normal modes by atmospheric winds and pressure fluctuations...
August 2016: Journal of the Acoustical Society of America
Andi Petculescu
Generic predictions for acoustic dispersion and absorption in the atmospheres of Mars and Venus are presented. For Mars, Pathfinder and Mars Express ambient data and averaged thermophysical parameters are used as inputs to a preliminary model based on the continuum approximation for Mars' thin atmosphere-the need for Boltzmann-based treatment is discussed in the context of Knudsen numbers. Strong absorption constrains acoustic sensing within the Martian planetary boundary layer. For the dense atmosphere of Venus, the van der Waals equation of state is used...
August 2016: Journal of the Acoustical Society of America
Michael A Ainslie, Timothy G Leighton
The set of formulations commonly known as "the sonar equations" have for many decades been used to quantify the performance of sonar systems in terms of their ability to detect and localize objects submerged in seawater. The efficacy of the sonar equations, with individual terms evaluated in decibels, is well established in Earth's oceans. The sonar equations have been used in the past for missions to other planets and moons in the solar system, for which they are shown to be less suitable. While it would be preferable to undertake high-fidelity acoustical calculations to support planning, execution, and interpretation of acoustic data from planetary probes, to avoid possible errors for planned missions to such extraterrestrial bodies in future, doing so requires awareness of the pitfalls pointed out in this paper...
August 2016: Journal of the Acoustical Society of America
Pranita Nirgudkar, Devin H Taylor, Yuchio Yanagawa, C Fernando Valenzuela
Cerebellar alterations are a hallmark of Fetal Alcohol Spectrum Disorders and are thought to be responsible for deficits in fine motor control, motor learning, balance, and higher cognitive functions. These deficits are, in part, a consequence of dysfunction of cerebellar circuits. Although the effect of developmental ethanol exposure on Purkinje and granule cells has been previously characterized, its actions on other cerebellar neuronal populations are not fully understood. Here, we assessed the impact of repeated ethanol exposure on the number of inhibitory neurons in the cerebellar vermis...
October 6, 2016: Neuroscience Letters
Ciera C Martinez, Daniel Koenig, Daniel H Chitwood, Neelima R Sinha
The spatiotemporal localization of the plant hormone auxin acts as a positional cue during early leaf and flower organogenesis. One of the main contributors to auxin localization is the auxin efflux carrier PIN-FORMED1 (PIN1). Phylogenetic analysis has revealed that PIN1 genes are split into two sister clades; PIN1 and the relatively uncharacterized Sister-Of-PIN1 (SoPIN1). In this paper we identify entire-2 as a loss-of-function SlSoPIN1a (Solyc10g078370) mutant in Solanum lycopersicum. The entire-2 plants are unable to specify proper leaf initiation leading to a frequent switch from the wild type spiral phyllotactic pattern to distichous and decussate patterns...
August 20, 2016: Developmental Biology
Kanwar Virdee, Jiska Kentrop, Bianca Jupp, Bethany Venus, Daniel Hensman, Simon McArthur, James Wilkinson, Trevor W Robbins, Glenda Gillies, Jeffrey W Dalley
RATIONALE: Antenatal exposure to the glucocorticoid dexamethasone dramatically increases the number of mesencephalic dopaminergic neurons in rat offspring. However, the consequences of this expansion in midbrain dopamine (DA) neurons for behavioural processes in adulthood are poorly understood, including working memory that depends on DA transmission in the prefrontal cortex (PFC). OBJECTIVES: We therefore investigated the influence of antenatal glucocorticoid treatment (AGT) on the modulation of spatial working memory by a D1 receptor agonist and on D1 receptor binding and DA content in the PFC and striatum...
October 2016: Psychopharmacology
Satoshi Shibata, Tsubasa Kawanai, Takayuki Hara, Asuka Yamamoto, Taro Chaya, Yasunori Tokuhara, Chinami Tsuji, Manabu Sakai, Taro Tachibana, Shinobu Inagaki
The function of ARHGEF10, a known guanine nucleotide exchange factor (GEF) for RhoA with proposed roles in various diseases, is poorly understood. To understand the precise function of this protein, we raised a monoclonal antibody against ARHGEF10 and determined its localization in HeLa cells. ARHGEF10 was found to localize to vesicles containing Rab6 (of which there are three isoforms, Rab6a, Rab6b and Rab6c), Rab8 (of which there are two isoforms, Rab8a and Rab8b), and/or the secretion marker neuropeptide Y (NPY)-Venus in a Rab6-dependent manner...
October 1, 2016: Journal of Cell Science
Rodolphe Dusaulcy, Sandra Handgraaf, Svetlana Skarupelova, Florian Visentin, Christian Vesin, Mounia Heddad-Masson, Frank Reimann, Fiona Gribble, Jacques Philippe, Yvan Gosmain
Glucose homeostasis depends on the coordinated secretion of glucagon, insulin, and Glucagon-like peptide (GLP)-1 by pancreas and intestine. Obesity, which is associated with an increased risk of developing insulin resistance and type 2 diabetes, affects the function of these organs. Here, we investigate the functional and molecular adaptations of proglucagon-producing cells in obese mice to better define their involvement in type 2 diabetes development. We used GLU-Venus transgenic male mice specifically expressing Venus fluorochrome in proglucagon-producing cells...
October 2016: Endocrinology
Jason Seth Rothman, Laszlo Kocsis, Etienne Herzog, Zoltan Nusser, Robin Angus Silver
Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine vesicle mobility at excitatory cerebellar mossy fibre synapses which sustain transmission over a broad frequency bandwidth. Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mice reveal 75% of VGLUT1-containing vesicles have a high mobility, comparable to that at ribbon synapses...
2016: ELife
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"