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Mechanical wound closure

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https://www.readbyqxmd.com/read/28529754/chitosan-poly-caprolactone-nanofibers-for-skin-repair
#1
Sheeny Lan Levengood, Ariane E Erickson, Fei-Chien Chang, Miqin Zhang
Dermal wounds, both acute and chronic, represent a significant clinical challenge and therefore the development of novel biomaterial-based skin substitutes to promote skin repair is essential. Nanofibers have garnered attention as materials to promote skin regeneration due to the similarities in morphology and dimensionality between nanofibers and native extracellular matrix proteins, which are critical in guiding cutaneous wound healing. Electrospun chitosan-poly(caprolactone) (CPCL) nanofiber scaffolds, which combine the important intrinsic biological properties of chitosan and the mechanical integrity and stability of PCL, were evaluated as skin tissue engineering scaffolds using a mouse cutaneous excisional skin defect model...
March 7, 2017: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/28478809/aloesin-from-aloe-vera-accelerates-skin-wound-healing-by-modulating-mapk-rho-and-smad-signaling-pathways-in-vitro-and-in-vivo
#2
Hussain Mustatab Wahedi, Minsun Jeong, Jae Kyoung Chae, Seon Gil Do, Hyeokjun Yoon, Sun Yeou Kim
BACKGROUND: Cutaneous wound healing is a complex process involving various regulatory factors at the molecular level. Aloe vera is widely used for cell rejuvenation, wound healing, and skin moisturizing. HYPOTHESIS/PURPOSE: This study aimed to investigate the effects of aloesin from Aloe vera on cutaneous wound healing and mechanisms involved therein. STUDY DESIGN: This study consisted of both in vitro and in vivo experiments involving skin cell lines and mouse model to demonstrate the wound healing effects of aloesin by taking into account several parameters ranging from cultured cell migration to wound healing in mice...
May 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28464475/isolation-of-cd248-expressing-stromal-vascular-fraction-for-targeted-improvement-of-wound-healing
#3
Elizabeth Brett, Elizabeth R Zielins, Monica Chin, Michael Januszyk, Charles P Blackshear, Michael Findlay, Arash Momeni, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
Wound healing remains a global issue of disability, cost, and health. Addition of cells from the stromal vascular fraction (SVF) of adipose tissue has been shown to increase the rate of full thickness wound closure. This study aimed to investigate the angiogenic mechanisms of CD248+ SVF cells in the context of full thickness excisional wounds. Single cell transcriptional analysis was used to identify and cluster angiogenic gene-expressing cells, which was then correlated with surface marker expression. SVF cells isolated from human lipoaspirate were FACS sorted based on the presence of CD248...
May 2, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28459539/berberine-induces-toxicity-in-hela-cells-through-perturbation-of-microtubule-polymerization-by-binding-to-tubulin-at-a-unique-site
#4
Darpan Raghav, Shabeeba M Ashraf, Lakshmi Mohan, Krishnan Rathinasamy
Berberine has been used traditionally for its diverse pharmacological actions. It exhibits remarkable anticancer activities and is currently under clinical trials. In this study, we report that the anticancer activity of berberine could be partly due to its inhibitory actions on tubulin and microtubule assembly. Berberine inhibited the proliferation of HeLa cells with an IC50 of 18 μM and induced significant depolymerization of interphase and mitotic microtubules. At its IC50, berberine exerted a moderate G2/M arrest and mitotic block as detected by fluorescence-activated cell sorting analysis and fluorescence microscopy, respectively...
May 4, 2017: Biochemistry
https://www.readbyqxmd.com/read/28454344/galectin-7-promotes-the-invasiveness-of-human-oral-squamous-cell-carcinoma-cells-via-activation-of-erk-and-jnk-signaling
#5
Jia-Ping Guo, Xiao-Guang Li
Galectin-7 is a member of the β-galactoside-binding protein family, and is highly expressed in oral squamous cell carcinoma (OSCC). The aim of the present study was to investigate the effects of manipulating galectin-7 expression on the biological phenotype of human OSCC cells and the associated molecular mechanisms. Knockdown of endogenous galectin-7 via small interfering RNA (siRNA) was performed and cell proliferation, apoptosis, migration, and invasion were subsequently assessed. The data indicated that galectin-7 silencing had no impact on the proliferation or apoptosis of OSCC cells...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28448267/endogenous-thymosin-%C3%AE-4-expression-in-sacrococcygeal-pilonidal-sinus-disease-a-retrospective-immunohistochemical-analysis-of-excisional-skin-biopsy-samples
#6
Sevinc Sahin
Thymosin beta 4 (Tβ4) is a peptide that has been shown in dermal, corneal, and cardiac preclinical injury models to potentially affect tissue protection, regeneration, and repair. Sacrococcygeal pilonidal sinus disease (SPSD) is a chronic inflammatory disorder associated with a high incidence of recurrence, chronic fistulation, and a challenging postoperative surgical wound healing process. Retrospectively, an immunohistochemical analysis was conducted to evaluate endogenous Tβ4 expression in excisional skin biopsies from patients with SPSD...
April 2017: Ostomy/wound Management
https://www.readbyqxmd.com/read/28421182/tgf-%C3%AE-1-pretreatment-improves-the-function-of-mesenchymal-stem-cells-in-the-wound-bed
#7
Deepraj Ghosh, Daniel J McGrail, Michelle R Dawson
The wound healing process initiates after injury to a tissue and involves a series of orchestrated events to minimize the invasion of foreign matters such as bacteria and efficiently regenerate the damaged tissue. A variety of cells must be recruited to the tissue during wound healing. However, this process is severely disrupted in patients suffering from chronic illness, including diabetes, leading to impaired healing or non-healing wounds. Current avenues of treatment include negative-pressure therapy, wound debridement, growth factor replacement, and cell-based therapies...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28396222/activin-b-regulates-adipose-derived-mesenchymal-stem-cells-to-promote-skin-wound-healing-via-activation-of-the-mapk-signaling-pathway
#8
Lei Zhang, Pengcheng Xu, Xueer Wang, Min Zhang, Yuan Yan, Yinghua Chen, Lu Zhang, Lin Zhang
Adipose-derived stem cells (ADSCs) are multipotent stromal cells that can differentiate into a variety of cell types, including skin cells, and they can provide an abundant source of cells for skin tissue engineering and skin wound healing. The purpose of this study is to explore the therapeutic effects of activin B in combination with ADSCs and the possible signaling mechanism. In this study, we found that activin B was able to promote ADSC migration by inducing actin stress fiber formation in vitro. In vivo, activin B in combination with ADSCs was capable of enhancing α-SMA expression and wound closure...
April 8, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28390267/effects-of-tgf%C3%AE-1-pdgf-bb-and-bfgf-on-human-corneal-fibroblasts-proliferation-and-differentiation-during-stromal-repair
#9
Patricia Gallego-Muñoz, Lucía Ibares-Frías, María Cruz Valsero-Blanco, Roberto Cantalapiedra-Rodriguez, Jesús Merayo-Lloves, M Carmen Martínez-García
In an effort to improve the regenerative nature of corneal repair, this study reports the use of an in vitro human corneal fibroblasts (HCFs) wound model after treatment with three of the main growth factors (GFs) involved in corneal healing: transforming growth factor beta 1 (TGFβ1), platelet-derived growth factor BB-isoform (PDGF-BB), and basic fibroblast growth factor (bFGF) in order to delve in cell proliferation and differentiation processes. HCFs were mechanically wounded. The individual effect of TGFβ1, PDGF-BB, and bFGF on cell proliferation and differentiation during the repair process was studied at different time points until wound closure...
April 5, 2017: Cytokine
https://www.readbyqxmd.com/read/28389357/abietic-acid-isolated-from-pine-resin-resina-pini-enhances-angiogenesis-in-huvecs-and-accelerates-cutaneous-wound-healing-in-mice
#10
Jun Yeon Park, Yun Kyung Lee, Dong-Soo Lee, Jeong-Eun Yoo, Myoung-Sook Shin, Noriko Yamabe, Su-Nam Kim, Seulah Lee, Ki Hyun Kim, Hae-Jeung Lee, Seok Sun Roh, Ki Sung Kang
ETHNOPHARMACOLOGICAL RELEVANCE: Resin known as Resina Pini is listed in the Korean and Japanese pharmacopoeias and has been used for treating skin wounds and inflammation. Resin is composed of more than 50% abietic acid and 10% neutral substances. OBJECTIVE: In the present study, the wound-healing effects of abietic acid and the possible underlying mechanism of action were investigated in various in vitro and in vivo models. MATERIALS AND METHODS: The effects of abietic acid on tube formation and migration were measured in human umbilical vein vascular endothelial cells (HUVECs)...
May 5, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28386568/mesenchymal-stem-cells-improve-healing-of-diabetic-foot-ulcer
#11
REVIEW
Yue Cao, Xiaokun Gang, Chenglin Sun, Guixia Wang
Mesenchymal stem cells (MSCs), an ideal cell source for regenerative therapy with no ethical issues, play an important role in diabetic foot ulcer (DFU). Growing evidence has demonstrated that MSCs transplantation can accelerate wound closure, ameliorate clinical parameters, and avoid amputation. In this review, we clarify the mechanism of preclinical studies, as well as safety and efficacy of clinical trials in the treatment of DFU. Bone marrow-derived mesenchymal stem cells (BM-MSCs), compared with MSCs derived from other tissues, may be a suitable cell type that can provide easy, effective, and cost-efficient transplantation to treat DFU and protect patients from amputation...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28345832/metformin-inhibits-migration-and-invasion-of-cholangiocarcinoma-cells
#12
Son Xuan Trinh, Huyen Thi Bich Nguyen, Kween Saimuang, Virapong Prachayasittikul, Waraporn Chan On
Background: Metformin is an oral anti-diabetic agent that has been widely prescribed for treatment of type II diabetes. Anti-cancer properties of metformin have been revealed for numerous human malignancies including cholangiocarcinoma (CCA) with anti-proliferative effects in vitro. However, effects on CCA cell migration and invasion have not been fully investigated. The present study aimed to explore the inhibitory effects of metformin on motility, migration and invasion of the CCA cell line HuCCT1, and examine molecular mechanisms underlying metformin effects...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28337222/a-conditioned-medium-of-umbilical-cord-mesenchymal-stem-cells-overexpressing-wnt7a-promotes-wound-repair-and-regeneration-of-hair-follicles-in-mice
#13
Liang Dong, Haojie Hao, Jiejie Liu, Dongdong Ti, Chuan Tong, Qian Hou, Meirong Li, Jingxi Zheng, Gang Liu, Xiaobing Fu, Weidong Han
Mesenchymal stem cells (MSCs) can affect the microenvironment of a wound and thereby accelerate wound healing. Wnt proteins act as key mediators of skin development and participate in the formation of skin appendages such as hair. The mechanisms of action of MSCs and Wnt proteins on skin wounds are largely unknown. Here, we prepared a Wnt7a-containing conditioned medium (Wnt-CM) from the supernatant of cultured human umbilical cord-MSCs (UC-MSCs) overexpressing Wnt7a in order to examine the effects of this CM on cutaneous healing...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28303029/tmf-and-glycitin-act-synergistically-on-keratinocytes-and-fibroblasts-to-promote-wound-healing-and-anti-scarring-activity
#14
Ga Young Seo, Yoongho Lim, Dongsoo Koh, Jung Sik Huh, Changlim Hyun, Young Mee Kim, Moonjae Cho
Keratinocyte-fibroblast interactions are critical for skin repair after injury. During the proliferative phase of wound healing, proliferation, migration and differentiation of these cells are the major mechanisms leading to tissue remodeling. We have previously reported that glycitin, a major soy isoflavone, stimulates dermal fibroblast proliferation; and the phytochemical, 4',6,7-trimethoxyisoflavone (TMF), induces migration of HaCaT keratinocyte cells. We therefore investigated whether these compounds display synergistic effects on skin cells during wound healing in vitro and in vivo...
March 17, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28292518/the-management-of-penetrating-rectal-and-anal-trauma-a-systematic-review
#15
Daniel P Ahern, Michael E Kelly, Danielle Courtney, Emanuele Rausa, Des C Winter
INTRODUCTION: Traumatic injuries to the lower gastrointestinal tract (rectum and anus) have been largely reported in the military setting with sparse publications from the civilian setting. Additionally, there remains a lack of international consensus regarding definitive treatment pathways. This systematic review aimed to assess the current literature and propose a standardised treatment algorithm to aid management in the civilian setting. METHODS: A systematic review of available literature from 1999 to 2016 that was performed...
June 2017: Injury
https://www.readbyqxmd.com/read/28282856/hepatitis-b-virus-x-protein-stimulates-proliferation-wound-closure-and-inhibits-apoptosis-of-huh-7-cells-via-cdc42
#16
Yongru Xu, Yingzi Qi, Jing Luo, Jing Yang, Qi Xie, Chen Deng, Na Su, Wei Wei, Deshun Shi, Feng Xu, Xiangping Li, Ping Xu
Chronic hepatitis B virus (HBV) infection has been considered as the major cause of hepatocellular carcinoma (HCC). Hepatitis B virus X protein (HBx) has been reported to be oncogenic. The underlying mechanisms of HBV-related HCC are not fully understood, and the role played by the HBx protein in HBV induced carcinogenesis remains controversial. CDC42, a member of the Rho GTPase family, has been reported to be overexpressed in several different cancers, including HBV-related HCC. However, the specific role of CDC42 in HCC development remains unclear...
March 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28268192/early-induction-of-nrf2-antioxidant-pathway-by-rhbdf2-mediates-rapid-cutaneous-wound-healing
#17
Vishnu Hosur, Lisa M Burzenski, Timothy M Stearns, Michelle L Farley, John P Sundberg, Michael V Wiles, Leonard D Shultz
Rhomboid family protein RHBDF2, an upstream regulator of the epidermal growth factor (EGF) receptor signaling, has been implicated in cutaneous wound healing. However, the underlying molecular mechanisms are still emerging. In humans, a gain-of-function mutation in the RHBDF2 gene accelerates cutaneous wound healing in an EGFR-dependent manner. Likewise, a gain-of-function mutation in the mouse Rhbdf2 gene (Rhbdf2(cub/cub)) shows a regenerative phenotype (rapid ear-hole closure) resulting from constitutive activation of the EGFR pathway...
April 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28257533/silk-derived-protein-enhances-corneal-epithelial-migration-adhesion-and-proliferation
#18
Waleed Abdel-Naby, Brigette Cole, Aihong Liu, Jingbo Liu, Pengxia Wan, Victor H Guaiquil, Ryan Schreiner, David Infanger, Brian D Lawrence, Mark I Rosenblatt
Purpose: The corneal surface is vulnerable to a myriad of traumatic insults including mechanical, chemical, and thermal injuries. The resulting trauma may render the naturally occurring regenerative properties of the cornea incapable of restoring a healthy epithelial surface, and may result in the loss of corneal transparency and vision. Healing of the corneal epithelium requires a complex cascade of biological processes that work to restore the tissue after injury. New therapeutic agents that act on the multiple steps of the corneal wound-healing process would offer a potential for improving patient outcomes...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28256293/use-of-a-home-vacuum-assisted-closure-device-in-the-burn-population-is-both-cost-effective-and-efficacious
#19
Oren P Mushin, Jarrod T Bogue, Mica D Esquenazi, Nicole Toscano, Derek E Bell
INTRODUCTION: The vacuum assisted closure device (VAC) improves wound-healing when utilized as a bolster to secure split thickness skin grafts (STSG). Patients typically remain hospitalized for VAC therapy; however, home VACs (hVAC) are now available. Limited studies examine burns treated with hVAC as a STSG bolster. METHOD: A retrospective study of records from an ABA verified regional burn center was conducted over 23 months. Patients included STSGs for burn. Data points included demographics, burn mechanism and location, graft characteristics, hospital length of stay (LOS), and time to heal...
February 27, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28245074/percutaneous-probe-stimulation-for-intraoperative-neuromonitoring-in-total-endoscopic-thyroidectomy-a-preliminary-experience
#20
Daqi Zhang, Fang Li, Che-Wei Wu, Xiaoli Liu, Jingwei Xin, Feng-Yu Chiang, Hui Sun
BACKGROUND: The purpose of this study was to investigate the feasibility and value of using intraoperative neuromonitoring (IONM) performed via percutaneous probe stimulation during total endoscopic thyroidectomy. METHODS: This study prospectively enrolled a series of 132 consecutive patients with 156 recurrent laryngeal nerves (RLNs) at risk who received total endoscopic thyroidectomy performed via bilateral breast approach using standardized IONM. The stimulation probe was introduced into the working space by percutaneous puncture...
May 2017: Head & Neck
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