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https://www.readbyqxmd.com/read/27904974/heat-stress-affects-carbohydrate-metabolism-during-cold-induced-sweetening-of-potato-solanum-tuberosum-l
#1
Derek J Herman, Lisa O Knowles, N Richard Knowles
Tolerance to heat stress for retention of low-temperature sweetening-resistant phenotype in potato is conferred by insensitivity of acid invertase activity to cold induction. Heat stress exacerbated cold sweetening (buildup of reducing sugars) of the LTS (low-temperature sweetening)-susceptible potato (Solanum tuberosum L.) cultivars, Ranger Russet and Russet Burbank, and completely abolished the resistance to cold sweetening in the LTS-resistant cultivars/clones, Sage Russet, GemStar Russet, POR06V12-3 and A02138-2...
November 30, 2016: Planta
https://www.readbyqxmd.com/read/27904601/evaluation-effects-of-nebulized-gentamicin-in-exacerbation-of-chronic-obstructive-lung-disease
#2
Forogh Soltaninejad, Soleiman Kheiri, Roya Habibian, Arshia Amra, Shahin Asgari-Savadjani
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a major cause of chronic morbidity and mortality throughout the world. Exacerbation of COPD has negative effect on quality of life. Therapeutic effect of nebulized antibiotics in pulmonary infections has been reported previously. Hence, we evaluated the effect of nebulized gentamicin in acute exacerbation of COPD (AECOPD). MATERIALS AND METHODS: In this clinical trial study, 86 hospitalized patients with AECOPD were divided into two groups for using nebulized gentamicin twice daily (case group) and placebo (control group) for 5 days in addition to standard treatment...
2016: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/27904410/involvement-of-%C3%AE-%C3%AE-and-%C3%AE-tocopherol-isomers-from%C3%A2-pumpkin-cucurbita-pepo-l-seed-oil-or-oil-mixtures-in%C3%A2-the-biphasic-dpph%C3%AB-disappearance-kinetics
#3
Dalibor Broznić, Gordana Čanadi Jurešić, Čedomila Milin
The antioxidant activity of three types of pumpkin seed oil or oil mixtures (cold- -pressed, produced from roasted seed paste and salad) produced in the northern part of Croatia and the kinetics of their behaviour as free radical scavengers were investigated using DPPH˙. In addition, the involvement of oil tocopherol isomers (α-, γ- and δ-) in different steps of DPPH˙ disappearance and their impact on the rate of reaction were analysed. The kinetics of DPPH˙ disappearance is a two-step process. In the first step, rapid disappearance of DPPH˙ occurs during the first 11 min of the reaction, depending on the oil type, followed by a slower decline in the second step...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27903702/cold-acclimation-improves-chill-tolerance-in-the-migratory-locust-through-preservation-of-ion-balance-and-membrane-potential
#4
Mads Kuhlmann Andersen, Rasmus Folkersen, Heath A MacMillan, Johannes Overgaard
Most insects have the ability to alter their cold tolerance in response to temporal temperature fluctuations, and recent studies have shown that insect cold tolerance is closely tied to the ability to maintain transmembrane ion-gradients that are important for the maintenance of cell membrane potential (Vm). Accordingly, several studies have suggested a link between preservation of Vm and cellular survival after cold stress, but none have measured Vm in this context. We tested this hypothesis by acclimating locusts (Locusta migratoria) to high (31°C) and low temperature (11°C) for four days before exposing them to cold stress (0°C) for up to 48 hours and subsequently measuring ion balance, cell survival, muscle Vm, and whole animal performance...
November 30, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27903513/the-maximum-heart-rate-in-brown-trout-salmo-trutta-fario-is-not-limited-by-firing-rate-of-pacemaker-cells
#5
Jaakko Haverinen, Denis V Abramochkin, Andre Kamkin, Matti Vornanen
Temperature-induced changes in cardiac output (Q) in fish are largely dependent on thermal modulation of heart rate (fH) and at high temperatures Q collapses due to heat-dependent depression of fH This study tests the hypothesis that firing rate of sinoatrial pacemaker cells sets the upper thermal limit of fH in vivo. To this end temperature-dependence of action potential (AP) frequency of enzymatically isolated pacemaker cells (pacemaker rate, fPM), spontaneous beating rate of isolated sinoatrial preparations (fSA) and in vivo heart rate (fH) of the cold-acclimated (4°C) brown trout (Salmo trutta fario) were compared under acute thermal challenges...
November 30, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/27903433/cold-acclimation-allows-drosophila-flies-to-maintain-mitochondrial-functioning-under-cold-stress
#6
Hervé Colinet, David Renault, Damien Roussel
Environmental stress generally disturbs cellular homeostasis. Researchers have hypothesized that chilling injury is linked to a shortage of ATP. However, previous studies conducted on insects exposed to nonfreezing low temperatures presented conflicting results. In this study, we investigated the mitochondrial bioenergetics of Drosophila melanogaster flies exposed to chronic cold stress (4 °C). We assessed mitochondrial oxygen consumption while monitoring the rate of ATP synthesis at various times (0, 1, 2, and 3 days) during prolonged cold stress and at two assay temperatures (25 and 4 °C)...
November 26, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27903363/-acute-effect-of-daily-mean-temperature-on-ischemic-heart-disease-mortality-a-multivariable-meta-analysis-from-12-counties-across-hubei-province-china
#7
Y Q Zhang, C H Yu, J Z Bao
Objective: To evaluate the acute effects of daily mean temperature on ischemic heart disease (IHD) mortality in 12 counties across Hubei Province, China. Methods: We obtained the daily IHD mortality data and meteorological data of the 12 counties for 2009-2012. The distributed lag nonlinear model (DLNM) was used to estimate the community-specific association between mean temperature and IHD mortality. A multivariate meta-analysis was then applied to pool the community-specific relationship between temperature and IHD mortality, and the effects of cold and heat on mortality risk...
November 6, 2016: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/27903054/future-malaria-spatial-pattern-based-on-the-potential-global-warming-impact-in-south-and-southeast-asia
#8
Hassan M Khormi, Lalit Kumar
We used the Model for Interdisciplinary Research on Climate-H climate model with the A2 Special Report on Emissions Scenarios for the years 2050 and 2100 and CLIMEX software for projections to illustrate the potential impact of climate change on the spatial distributions of malaria in China, India, Indochina, Indonesia, and The Philippines based on climate variables such as temperature, moisture, heat, cold and dryness. The model was calibrated using data from several knowledge domains, including geographical distribution records...
November 21, 2016: Geospatial Health
https://www.readbyqxmd.com/read/27903009/temperature-controlled-continuous-cold-flow-device-after-total-knee-arthroplasty-a-randomized-controlled-trial-study
#9
Alberto Ruffilli, Francesco Castagnini, Francesco Traina, Isabella Corneti, Domenico Fenga, Sandro Giannini, Cesare Faldini
Total knee arthroplasty (TKA) is a widely accepted and successful procedure for end-stage arthritis. Nevertheless, fast-track may be compromised by many factors, such as pain, edema, and blood loss. Cryotherapy has been advocated as a safe and effective strategy to improve the postoperative results, acting on pain, edema, and blood loss. This study is a prospective randomized controlled study, involving 50 patients after primary TKA. A power analysis was performed preoperatively. Twenty-four patients were addressed to a postoperative treatment with a continuous cold flow device (Hilotherm, Hilotherm GmbH, Germany)...
November 30, 2016: Journal of Knee Surgery
https://www.readbyqxmd.com/read/27902803/alphasim-software-for-breeding-program-simulation
#10
Anne-Michelle Faux, Gregor Gorjanc, R Chris Gaynor, Mara Battagin, Stefan M Edwards, David L Wilson, Sarah J Hearne, Serap Gonen, John M Hickey
This paper describes AlphaSim, a software package for simulating plant and animal breeding programs. AlphaSim enables the simulation of multiple aspects of breeding programs with a high degree of flexibility. AlphaSim simulates breeding programs in a series of steps: (i) simulate haplotype sequences and pedigree; (ii) drop haplotypes into the base generation of the pedigree and select single-nucleotide polymorphism (SNP) and quantitative trait nucleotide (QTN); (iii) assign QTN effects, calculate genetic values, and simulate phenotypes; (iv) drop haplotypes into the burn-in generations; and (v) perform selection and simulate new generations...
November 2016: Plant Genome
https://www.readbyqxmd.com/read/27902716/large-scale-anthropogenic-reduction-of-forest-cover-in-last-glacial-maximum-europe
#11
Jed O Kaplan, Mirjam Pfeiffer, Jan C A Kolen, Basil A S Davis
Reconstructions of the vegetation of Europe during the Last Glacial Maximum (LGM) are an enigma. Pollen-based analyses have suggested that Europe was largely covered by steppe and tundra, and forests persisted only in small refugia. Climate-vegetation model simulations on the other hand have consistently suggested that broad areas of Europe would have been suitable for forest, even in the depths of the last glaciation. Here we reconcile models with data by demonstrating that the highly mobile groups of hunter-gatherers that inhabited Europe at the LGM could have substantially reduced forest cover through the ignition of wildfires...
2016: PloS One
https://www.readbyqxmd.com/read/27902347/the-conserved-n-terminus-of-human-rhinovirus-capsid-protein-vp4-contains-membrane-pore-forming-activity-and-is-a-target-for-neutralising-antibodies
#12
Anusha Panjwani, Amin S Asfor, Tobias J Tuthill
Human rhinovirus is the causative agent of the common cold and belongs to the non-enveloped picornavirus family. A trigger such as receptor binding or low pH initiates conformational changes in the capsid that allow the virus to attach to membranes and form a pore for the translocation of viral RNA into the cytoplasm. We previously showed that recombinant capsid protein VP4 was able to form membrane pores. In this study, we show the N-terminus but not C-terminus of VP4, formed pores with properties similar to full-length VP4 and consistent with the size required for transfer of RNA...
October 11, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27902239/mucilaginibacter-psychrotolerans-sp-nov-isolated-from-the-riganqiao-peatlands-on-the-tibetan-plateau
#13
Yongcui Deng, Liang Shen, Baiqin Xu, Yongqin Liu, Zhengquan Gu, Hongcan Liu, Yuguang Zhou
A Gram-stain-negative, rod-shaped without flagella, pink colored, cold-tolerate bacteria strain NH7-4T was isolated from Riganqiao peatlands on the Tibetan Plateau. The novel isolate was classified into the genus Mucilaginibacter. 16S rRNA gene sequence of the novel isolate shares a pairwise similarity ranging from 96.69% to 93.41% with the type strains of the genus Mucilaginibacter. Growth of strain NH7-4T occurred between 0 and 30 °C and at pH 5.0-9.0, with optimum at 20 °C and pH about 7.0. The major isoprenoid quinone is MK-7...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902194/flavobacterium-orientale-sp-nov-isolated-from-lake-water
#14
Ai-Hua Li, Hong-Can Liu, Yu-Guang Zhou
Two Gram-stain-negative, obligately aerobic, non-motile, rod-shaped bacterial strains, designated SP3T and SP38, were isolated from a cold-water lake, in the west of China. Polyphasic taxonomy was performed for the strains. Alignment of 16S rRNA gene sequences indicated that strains SP3T and SP38 were associated with the genus Flavobacterium and were most closely related to Flavobacterium lacus NP180T (96.4 % sequence similarity), Flavobacterium ponti GSW-R14T (95.6 %) and Flavobacterium yanchengense hgT (95...
October 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901499/genetic-characterization-of-esocid-herpesvirus-1-eshv1
#15
Jared T Freitas, Kuttichantran Subramaniam, Karen L Kelley, Susan Marcquenski, Joseph Groff, Thomas B Waltzek
Blue spot disease, believed to be caused by esocid herpesvirus 1 (EsHV1), has been observed in wild northern pike Esox lucius in a number of cold-water locations, including the northern USA, Canada, and Ireland. In the spring of 2014, a northern pike was caught in Wisconsin displaying the characteristic bluish-white circular plaques on the dorsum and fins. Microscopic examination of hematoxylin and eosin-stained sections of the proliferative cutaneous lesions revealed a focally extensive abundance of panepidermal, megalocytic keratinocytes with karyomegaly...
November 22, 2016: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/27901336/robotic-kidney-transplantation-current-status-and-future-perspectives
#16
Angelo Territo, Alex Mottrie, Ronney Abaza, Craig Rogers, Mani Menon, Mahendra Bhandari, Rajesh Ahlawat, Alberto Breda
INTRODUCTION: For the treatment of patients with end-stage renal disease, kidney transplantation is preferred to renal replacement modalities such as hemodialysis and peritoneal dialysis. Although open surgery remains the gold standard, minimally invasive approaches have recently been applied in transplant kidney surgery. Despite growing enthusiasm and potential benefits of robotic kidney transplant, many aspects of this novel technique remain controversial. Aim of this study was to analyze the current status and future developments in robotic-assisted surgery for kidney transplantation...
November 30, 2016: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/27901019/additives-for-vaccine-storage-to-improve-thermal-stability-of-adenoviruses-from-hours-to-months
#17
Maria Pelliccia, Patrizia Andreozzi, Jayson Paulose, Marco D'Alicarnasso, Valeria Cagno, Manuela Donalisio, Andrea Civra, Rebecca M Broeckel, Nicole Haese, Paulo Jacob Silva, Randy P Carney, Varpu Marjomäki, Daniel N Streblow, David Lembo, Francesco Stellacci, Vincenzo Vitelli, Silke Krol
Up to 80% of the cost of vaccination programmes is due to the cold chain problem (that is, keeping vaccines cold). Inexpensive, biocompatible additives to slow down the degradation of virus particles would address the problem. Here we propose and characterize additives that, already at very low concentrations, improve the storage time of adenovirus type 5. Anionic gold nanoparticles (10(-8)-10(-6) M) or polyethylene glycol (PEG, molecular weight ∼8,000 Da, 10(-7)-10(-4) M) increase the half-life of a green fluorescent protein expressing adenovirus from ∼48 h to 21 days at 37 °C (from 7 to >30 days at room temperature)...
November 30, 2016: Nature Communications
https://www.readbyqxmd.com/read/27900683/constructing-and-screening-a-metagenomic-library-of-a-cold-and-alkaline-extreme-environment
#18
Mikkel A Glaring, Jan K Vester, Peter Stougaard
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27900382/ultra-fast-preparation-of-high-performance-thermoelectric-bulk-tinisb0-05sn0-95-by-microwave-synthesis
#19
Y Li, C Cheng, Y Lei, M Wang, R D Wan
The antimony-doped half-Heusler thermoelectric bulk TiNiSb0.05Sn0.95 was prepared via ultra-fast 4.5 min microwave synthesis, cold-press forming and 20 min microwave pressureless sintering. The electrical properties i.e. the Seebeck coefficient (S), electrical resistivity (ρ), and carrier concentration (n) and mobility (μ) were measured on a Seebeck coefficient/resistance analysis system (S/RAs) and Hall effect measurement system (HEMS), respectively. The thermal conductivity (κ) was measured on a laser flash thermal analyzer (LFA)...
November 30, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27899893/raynaud-s-phenomenon-a-brief-review-of-the-underlying-mechanisms
#20
REVIEW
Manal M Fardoun, Joseph Nassif, Khodr Issa, Elias Baydoun, Ali H Eid
Raynaud's phenomenon (RP) is characterized by exaggerated cold-induced vasoconstriction. This augmented vasoconstriction occurs by virtue of a reflex response to cooling via the sympathetic nervous system as well as by local activation of α2C adrenoceptors (α2C-AR). In a cold-initiated, mitochondrion-mediated mechanism involving reactive oxygen species and the Rho/ROCK pathway, cytoskeletal rearrangement in vascular smooth muscle cells orchestrates the translocation of α2C-AR to the cell membrane, where this receptor readily interacts with its ligand...
2016: Frontiers in Pharmacology
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