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https://www.readbyqxmd.com/read/29332154/the-influence-of-systemically-or-locally-administered-mesenchymal-stem-cells-on-tissue-repair-in-a-rat-oral-implantation-model
#1
Miya Kanazawa, Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Ryosuke Kondo, Yuri Matsuura, Kiyoshi Koyano
BACKGROUND: Multipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigated differences in treatment effect between local and systemic MSC application using a rat oral implantation model...
January 13, 2018: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/29326119/association-of-mutations-with-morphologic-dysplasia-in-de-novo-acute-myeloid-leukemia-without-2016-who-classification-defined-cytogenetic-abnormalities
#2
Olga K Weinberg, Christopher J Gibson, Traci M Blonquist, Donna Neuberg, Olga Pozdnyakova, Frank Kuo, Benjamin L Ebert, Robert P Hasserjian
Despite improvements in our understanding of the molecular basis of acute myeloid leukemia, the association between genetic mutations with morphologic dysplasia remains unclear. In this study, we evaluated and scored dysplasia in bone marrow specimens from 168 patients with de novo acute myeloid leukemia; none of these patients had 2016 WHO Classification-defined cytogenetic abnormalities. We then performed targeted sequencing of diagnostic bone marrow aspirates for recurrent mutations associated with myeloid malignancies...
January 11, 2018: Haematologica
https://www.readbyqxmd.com/read/29321693/human-mesenchymal-stem-cells-lose-their-functional-properties-after-paclitaxel-treatment
#3
Franziska Münz, Ramon Lopez Perez, Thuy Trinh, Sonevisay Sisombath, Klaus-Josef Weber, Patrick Wuchter, Jürgen Debus, Rainer Saffrich, Peter E Huber, Nils H Nicolay
Mesenchymal stem cells (MSCs) are an integral part of the bone marrow niche and aid in the protection, regeneration and proliferation of hematopoietic stem cells after exposure to myelotoxic taxane anti-cancer agents, but the influence of taxane compounds on MSCs themselves remains incompletely understood. Here, we show that bone marrow-derived MSCs are highly sensitive even to low concentrations of the prototypical taxane compound paclitaxel. While MSCs remained metabolically viable, they were strongly impaired regarding both their proliferation and their functional capabilities after exposure to paclitaxel...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321040/automated-image-analysis-detects-aging-in-clinical-grade-mesenchymal-stromal-cell-cultures
#4
S Oja, P Komulainen, A Penttilä, J Nystedt, M Korhonen
BACKGROUND: Senescent cells are undesirable in cell therapy products due to reduced therapeutic activity and risk of aberrant cellular effects, and methods for assessing senescence are needed. Early-passage mesenchymal stromal cells (MSCs) are known to be small and spindle-shaped but become enlarged upon cell aging. Indeed, cell morphology is routinely evaluated during MSC production using subjective methods. We have therefore explored the possibility of utilizing automated imaging-based analysis of cell morphology in clinical cell manufacturing...
January 10, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29305041/arthroscopic-treatment-of-osteochondral-lesions-of-the-talus-using-juvenile-articular-cartilage-allograft-and-autologous-bone-marrow-aspirate-concentration
#5
Bridget A DeSandis, Amgad M Haleem, Carolyn M Sofka, Martin J O'Malley, Mark C Drakos
Juvenile allogenic chondrocyte implantation (JACI; DeNovo NT Natural Tissue Graft®; Zimmer, Warsaw, IN) with autologous bone marrow aspirate concentrate (BMAC) is a relatively new all-arthroscopic procedure for treating critical-size osteochondral lesions (OCLs) of the talus. Few studies have investigated the clinical and radiographic outcomes of this procedure. We collected the clinical and radiographic outcomes of patients who had undergone JACI-BMAC for talar OCLs to assess treatment efficacy and cartilage repair tissue quality using magnetic resonance imaging (MRI)...
January 2, 2018: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29297135/stem-cell-factor-supports-migration-in-canine-mesenchymal-stem-cells
#6
Nathaly Enciso, Luciana L K Ostronoff, Guillermo Mejías, Leticia G León, María Luisa Fermín, Elena Merino, Cristina Fragio, Luis Avedillo, Concepción Tejero
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC)...
January 3, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29296920/identification-of-a-murine-cd45-f4-80lo-hsc-derived-marrow-endosteal-cell-associated-with-donor-stem-cell-engraftment
#7
Kathleen M Overholt, Satoru Otsuru, Timothy S Olson, Adam J Guess, Victoria M Velazquez, Laura Desbourdes, Massimo Dominici, Edwin M Horwitz
Hematopoietic stem cells (HSCs) reside in specialized microenvironments within the marrow designated as stem cell niches, which function to support HSCs at homeostasis and promote HSC engraftment after radioablation. We previously identified marrow space remodeling after hematopoietic ablation, including osteoblast thickening, osteoblast proliferation, and megakaryocyte migration to the endosteum, which is critical for effective engraftment of donor HSCs. To further evaluate the impact of hematopoietic cells on marrow remodeling, we used a transgenic mouse model (CD45Cre/iDTR) to selectively deplete hematopoietic cells in situ...
December 26, 2017: Blood Advances
https://www.readbyqxmd.com/read/29296759/a-specialized-pathway-for-erythroid-iron-delivery-through-lysosomal-trafficking-of-transferrin-receptor-2
#8
Shadi Khalil, Maja Holy, Stephen Grado, Robert Fleming, Ryo Kurita, Yukio Nakamura, Adam Goldfarb
Erythroid progenitors are the largest consumers of iron in the human body. In these cells, a high flux of iron must reach the mitochondrial matrix to form sufficient heme to support hemoglobinization. Canonical erythroid iron trafficking occurs via the first transferrin receptor (TfR1)-mediated endocytosis of diferric-transferrin into recycling endosomes, where ferric iron is released, reduced, and exported to the cytosol via DMT1. However, mice lacking TfR1 or DMT1 demonstrate residual erythropoiesis, suggesting additional pathways for iron use...
June 27, 2017: Blood Advances
https://www.readbyqxmd.com/read/29288042/stat3-mutation-and-its-clinical-and-histopathologic-correlation-in-t-cell-large-granular-lymphocytic-leukemia
#9
Min Shi, Rong He, Andrew L Feldman, David S Viswanatha, Dragan Jevremovic, Dong Chen, William G Morice
Although T-cell large granular lymphocytic leukemia (T-LGLL) is a clinically indolent disorder, patients with moderate-to-severe cytopenia require therapeutic intervention. The recent discovery of STAT3 mutations has shed light on the genetic basis of T-LGLL pathogenesis. However, the association of STAT3 mutational status with patients' clinical, histopathologic, and other laboratory features has not been thoroughly evaluated in T-LGLL. In this study, STAT3 mutations were identified in 18 of 36 patients with T-LGLL (50%), including Y640F (12/18, 66...
December 26, 2017: Human Pathology
https://www.readbyqxmd.com/read/29287231/degradation-of-poly-%C3%AE%C2%B5-caprolactone-and-bio-interactions-with-mouse-bone-marrow-mesenchymal-stem-cells
#10
Sukanya V S, Mohanan P V
Bio-inspired scaffolds in bone tissue engineering using multipotential mesenchymal stem cells grow at a rapid rate found its successful use in orthopedic injury treatment. Poly(ε-caprolactone)/PCL is widely used in medical devices, tissue engineering, and drug delivery systems. Most desirable property of biodegradable polymer to be employed in medical application is synchronization of degradation with functional tissue regeneration. Limited studies have incorporated the degradation kinetics and implication of degradation products of pure unmodified PCL...
December 21, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29285145/comparison-of-bone-marrow-vs-adipose-tissue-derived-mesenchymal-stem-cells-for-attenuating-liver-fibrosis
#11
Tianpao Hao, Jingfeng Chen, Shaoce Zhi, Qiyu Zhang, Gang Chen, Fuxiang Yu
Mesenchymal stem cell (MSC) therapy has emerged as a potential novel method of treating liver fibrosis. To date, bone marrow-derived MSCs (BM-MSCs) and adipose tissue-derived MSCs (AD-MSCs) have not been analyzed with respect to their ability to combat liver fibrosis. The present study aimed to compare the capabilities of BM-MSCs and AD-MSCs in the treatment of liver fibrosis. BM-MSCs and AD-MSCs were taken from male Sprague-Dawley rats and cultured. Hepatic stellate cells (HSCs) were co-cultured with either BM-MSCs or AD-MSCs, and the effects of BM-MSCs or AD-MSCs on the proliferation, activation and apoptosis of HSCs were determined...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29285060/inhibitory-effect-of-oxidative-damage-on-cardiomyocyte-differentiation-from-wharton-s-jelly-derived-mesenchymal-stem-cells
#12
Natakarn Nimsanor, Jitrada Phetfong, Chotiros Plabplueng, Kulachart Jangpatarapongsa, Virapong Prachayasittikul, Aungkura Supokawej
Ischemic heart diseases are a serious health problem worldwide. The transplantation of mesenchymal stem cells (MSCs) has been investigated in numerous clinical trials on various other diseases due to the self-renewal capacity of these cells and their potential to differentiate into a variety of cell types. The presence of excess reactive oxygen species in injured myocardium causes cardiac dysfunction and leads to inefficient repair of the heart. The poor outcomes of stem cell transplantation have been suggested to result from residual oxidative damage affecting the transplanted cells...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29284777/assessment-of-the-effect-of-wave-device-application-on-morphological-changes-in-organs-and-cells-of-irradiated-animals
#13
V Bebeshko, I Homolyako, V Grynchyshyn
AIM: To study the effect of the Device for wave influence on biological objects on the prevention of the development of acute radiation sickness and chronic radiation syndrome in vivo. MATERIALS AND METHODS: The studies were performed on white rats irradiated at a dose of 8 Gy. The experimental group of irradiated rats was treated with a wave Device (Patent of Ukraine No. 53568) once, for 2.5 min, 1.5 h after irradiation. Their organs were processed by standard histologic methods...
December 2017: Experimental Oncology
https://www.readbyqxmd.com/read/29282219/mutant-calreticulin-knock-in-mice-develop-thrombocytosis-and-myelofibrosis-without-a-stem-cell-self-renewal-advantage
#14
Juan Li, Daniel Prins, Hyun Jung Park, Jacob Grinfeld, Carlos Gonzalez-Arias, Stephen Loughran, Oliver M Dovey, Thorsten Klampfl, Cavan Bennett, Tina L Hamilton, Dean C Pask, Rachel Sneade, Matthew Williams, Juliet Aungier, Cedric Ghevaert, George S Vassiliou, David G Kent, Anthony R Green
Somatic mutations in the endoplasmic reticulum chaperone calreticulin (CALR) are detected in approximately 40% of patients with essential thrombocythemia (ET) and primary myelofibrosis (PMF). Multiple different mutations have been reported, but all result in a +1bp frame shift and generate a novel protein C-terminus. In this study, we generated a conditional mouse knock-in model of the most common CALR mutation, a 52bp deletion. The mutant novel human C-terminal sequence is integrated into the otherwise intact mouse CALR gene and results in mutant CALR expression under the control of the endogenous mouse locus...
December 27, 2017: Blood
https://www.readbyqxmd.com/read/29278963/a-plasma-cell-myeloma-with-post-therapy-anaplastic-morphology-osteomyelosclerosis-and-strong-pan-cytokeratin-ae1-ae3-expression-a-potential-diagnostic-pitfall
#15
Vani Bharani, Prashant Sharma, Amanjit Bal, Gaurav Prakash
Reactive and neoplastic plasma cells can display considerable morphological anaplasia as well as variable immunoreactivity for epithelial markers including epithelial membrane antigen, pan-cytokeratin (panCK) and high-molecular-weight cytokeratin, potentially creating diagnostic dilemmas. We describe the case of a 51-year-old male, previously treated for IgGλ plasma cell myeloma, whose bone marrow biopsy showed focal replacement by sheets of pleomorphic malignant cells and grade 3 myelofibrosis, raising the morphological possibility of a carcinomatous infiltration...
December 1, 2017: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29278889/erythromyeloid-derived-trem2-a-major-determinant-of-alzheimer-s-disease-pathology-in-down-syndrome
#16
Ruma Raha-Chowdhury, James W Henderson, Animesh Alexander Raha, Simon R W Stott, Romina Vuono, Simona Foscarin, Liam Wilson, Tiina Annus, Robert Fincham, Kieren Allinson, Vinod Devalia, Robert P Friedland, Anthony Holland, Shahid H Zaman
BACKGROUND: Down syndrome (DS; trisomy 21) individuals have a spectrum of hematopoietic and neuronal dysfunctions, and by the time they reach the age of 40 years, almost all develop Alzheimer's disease (AD) neuropathology which includes senile plaques and neurofibrillary tangles. Inflammation and innate immunity are key players in AD and DS. Triggering receptor expressed in myeloid cells-2 (TREM2) variants have been identified as risk factors for AD and other neurodegenerative diseases...
December 16, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29261159/a-rare-case-of-pure-erythroid-sarcoma-in-a-pediatric-patient-case-report-and-literature-review
#17
Pablo Manresa, Fabián Tarín, María Niveiro, María Tasso, Olga Alda, Francisco López, Héctor Sarmiento, José J Verdú, Francisco De Paz, Silvia López, María Del Cañizo, Esperanza Such, Eva Barragán, Fernanda Martirena
We describe an exceptional case of erythroid sarcoma in a pediatric patient as a growing orbital mass with no evidence of morphologic bone marrow involvement, who was finally diagnosed of pure erythroid sarcoma based on histopathology and flow cytometry criteria. We discuss the contribution of standardized eight-color flow cytometry as a rapid and reliable diagnostic method. The use of normal bone marrow databases allowed us to identify small aberrant populations in bone marrow and later confirm the diagnosis in the neoplastic tissue...
December 20, 2017: Children
https://www.readbyqxmd.com/read/29260516/-effect-of-recombinant-antigen-ferritin-on-dc-induce-immune-response
#18
Ya-Na Wang, Qiang Wang, Chan Wang, Wei Zhao
OBJECTIVE: To explore the immunoreaction against protoscolex infection by the recombinant ferritin of echinococcus granulosus (rEg.ferritin) immunized mice. METHODS: Bone marrow deriedstem cells (BMDCs) were collected and exposed in different antigens by cell culture and included 6 groups,there were hydatid fluid (HF) group (HF 30 μg/mL),rEg.ferritin group (rEg.ferritin 1 μg/mL), rEg.ferritin+LPS group (rEg.ferritin 1 μg/mL and LPS 100 ng/mL),HF+LPS group (HF 30 μg/mL and LPS 100 ng/mL),LPS group (LPS 100 ng/mL) and control (not add antigen)...
November 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29250536/transient-receptor-potential-melastatin-2-negatively-regulates-lps-atp-induced-caspase-1-dependent-pyroptosis-of-bone-marrow-derived-macrophage-by-modulating-ros-production
#19
Haihong Wang, Xinyi Zhou, Hui Li, Xiaowei Qian, Yan Wang, Liang Ma
Background: Pyroptosis, a new form of cell death, which has special morphological characteristics, depends on caspase-1 activation and occupies an important role in inflammatory immune diseases and ischemia-reperfusion injury. ROS is a common activator of NLR/caspase-1. Transient receptor potential melastatin 2 (TRPM2), a selective cation channel, is involved in inflammatory regulation. This study was designed to explore the role of TRPM2 in activating caspase-1 and caspase-1-dependent pyroptosis of mouse BMDMs...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29249823/flow-cytometric-vs-morphologic-assessment-of-remission-in-childhood-acute-lymphoblastic-leukemia-a-report-from-the-children-s-oncology-group-cog
#20
S Gupta, M Devidas, M L Loh, E A Raetz, S Chen, C Wang, P Brown, A J Carroll, N A Heerema, J M Gastier-Foster, K P Dunsmore, E C Larsen, K W Maloney, L A Mattano, S S Winter, N J Winick, W L Carroll, S P Hunger, M Borowitz, B L Wood
Minimal residual disease (MRD) after initial therapy is integral to risk stratification in B- and T-precursor acute lymphoblastic leukemia (B-ALL, T-ALL). Although MRD determines depth of remission, remission remains defined by morphology. We determined the outcomes of children with discordant assessments of remission by morphology vs flow cytometry using patients aged 1-30.99 years enrolled on Children's Oncology Group ALL trials who underwent bone marrow assessment at the end of induction (N=9350). Morphologic response was assessed locally as M1 (<5% lymphoblasts; remission), M2 (5-25%) or M3 (>25%)...
December 18, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
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