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Marrow morphology

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https://www.readbyqxmd.com/read/28731172/comparison-of-the-neuronal-differentiation-abilities-of-bone-marrow%C3%A2-derived-and-adipose-tissue%C3%A2-derived-mesenchymal-stem-cells
#1
Yani Zheng, Chao Huang, Fang Liu, Haiyan Lin, Xiangqun Yang, Zhiying Zhang
Bone marrow‑derived mesenchymal stem cells (BMSCs) and adipose tissue‑derived mesenchymal stem cells (ADSCs) are able to differentiate into neuron‑like cells when exposed to small molecule compounds, however the specific differences in their neuronal differentiation abilities remain to be fully elucidated. The present study aimed to compare the neuronal differentiation abilities of BMSCs and ADSCs. BMSCs and ADSCs from the same Sprague Dawley rats were isolated and cultured for use. The proliferation capacity was revealed using a cell counting method...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28730782/human-glioma-cells-conditioned-medium-promotes-proliferation-and-migration-of-human-bone-marrow-mesenchymal-stromal-cells-in-vitro
#2
Rusen Zhu, Xinggui Chen, Jinghong Li, Liubo Lan
PURPOSE: To investigate changes in cell proliferation and migration of human bone marrow-derived mesenchymal stromal cells (hMSCs) in glioma environment, in order to assess the tumorigenic risk of hMSCs in the clinical application to treat human gliomas. METHODS: hMSCs were obtained from normal adult persons and identified by their morphological characteristics and test of their stemness. The U251 glioma cell-conditioned medium (U251-CM) was obtained to simulate the human glioma environment in vitro...
May 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28730545/platelet-first-responders-in-wound-response-cancer-and-metastasis
#3
REVIEW
David G Menter, Scott Kopetz, Ernest Hawk, Anil K Sood, Jonathan M Loree, Paolo Gresele, Kenneth V Honn
Platelets serve as "first responders" during normal wounding and homeostasis. Arising from bone marrow stem cell lineage megakaryocytes, anucleate platelets can influence inflammation and immune regulation. Biophysically, platelets are optimized due to size and discoid morphology to distribute near vessel walls, monitor vascular integrity, and initiate quick responses to vascular lesions. Adhesion receptors linked to a highly reactive filopodia-generating cytoskeleton maximizes their vascular surface contact allowing rapid response capabilities...
July 20, 2017: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/28729432/tropomodulin1-controls-erythroblast-enucleation-via-regulation-of-f-actin-in-the-enucleosome
#4
Roberta B Nowak, Julien Papoin, David S Gokhin, Carla Casu, Stefano Rivella, Jeffrey M Lipton, Lionel Blanc, Velia M Fowler
Biogenesis of mammalian red blood cells requires nuclear expulsion by orthochromatic erythoblasts late in terminal differentiation (enucleation), but the mechanism is largely unexplained. Here, we employed high resolution confocal microscopy to analyze nuclear morphology and F-actin rearrangements during the initiation, progression and completion of mouse and human erythroblast enucleation in vivo Mouse erythroblast nuclei acquire a dumbbell-shaped morphology during enucleation, while human bone marrow erythroblast nuclei unexpectedly retain their spherical morphology...
July 20, 2017: Blood
https://www.readbyqxmd.com/read/28728747/endothelial-to-mesenchymal-transition-in-bone-marrow-and-spleen-of-primary-myelofibrosis
#5
Benedetta Gaia Erba, Cristian Gruppi, Monica Corada, Federica Pisati, Vittorio Rosti, Niccolo' Bartalucci, Jean-Luc Villeval, Alessandro Maria Vannucchi, Giovanni Barosi, Alessandra Balduini, Elisabetta Dejana
Primary myelofibrosis is characterized by the development of fibrosis in the bone marrow that contributes to ineffective hematopoiesis. Bone marrow fibrosis is the result of a complex and not yet fully understood interaction among megakaryocytes, myeloid cells, fibroblasts, and endothelial cells. Here, we report that >30% of the endothelial cells in the small vessels of the bone marrow and spleen of patients with primary myelofibrosis have a mesenchymal phenotype, which is suggestive of the process known as endothelial-to-mesenchymal transition (EndMT)...
August 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28716680/three-dimensional-adiabatic-inversion-recovery-prepared-ultrashort-echo-time-cones-3d-ir-ute-cones-imaging-of-cortical-bone-in-the-hip
#6
Amin Nazaran, Michael Carl, Yajun Ma, Saeed Jerban, Yanchun Zhu, Xing Lu, Jiang Du, Eric Y Chang
PURPOSE: We present three-dimensional adiabatic inversion recovery prepared ultrashort echo time Cones (3D IR-UTE-Cones) imaging of cortical bone in the hip of healthy volunteers using a clinical 3T scanner. METHODS: A 3D IR-UTE-Cones sequence, based on a short pulse excitation followed by a 3D Cones trajectory, with a nominal TE of 32μs, was employed for high contrast morphological imaging of cortical bone in the hip of heathy volunteers. Signals from soft tissues such as muscle and marrow fat were suppressed via adiabatic inversion and signal nulling...
July 14, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28714177/curved-microstructures-promote-osteogenesis-of-mesenchymal-stem-cells-via-the-rhoa-rock-pathway
#7
Qi Zhang, Shiyu Lin, Tao Zhang, Taoran Tian, Quanquan Ma, Xueping Xie, Changyue Xue, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: Cells in the osteon reside in a curved space, accordingly, the curvature of the microenvironment is an important geometric feature in bone formation. However, it is not clear how curved microstructures affect cellular behaviour in bone tissue. MATERIALS AND METHODS: Rat primary bone marrow mesenchymal stem cells (BMSCs) on wavy microgrooves were exposed to PDMS substrates with various curvatures to investigate alterations in cellular morphology and osteogenic differentiation...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28714031/effects-of-neuritin-on-the-differentiation-of-bone-marrow%C3%A2-derived-mesenchymal-stem-cells-into-neuron%C3%A2-like-cells
#8
Jingling Zhu, Pingping Meng, Qian Wang, Haiyan Wang, Jinli Zhang, Yuanyuan Li, Dongzheng Li, Xiaohua Tan, Lei Yang, Jin Huang
While the neurotrophic factor neuritin is known to be involved in neurodevelopment, the effects of this compound on cell differentiation remain unclear. The present study demonstrated that neuritin treatment induced the differentiation of rat bone marrow‑derived mesenchymal stem cells (rBM‑MSCs) into neuron‑like (NL) cells. For these analyses, rBM‑MSCs were incubated with 0.5 µg/ml neuritin for 24 h. Following induction, 27% of the rBM‑MSCs exhibited typical NL cell morphologies. Subsequently, NL cells were characterized by examining the expression of neuronal markers and by analysis of cell functions...
July 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713710/the-healing-effect-of-conditioned-media-and-bone-marrow-derived-stem-cells-in-laryngotracheal-stenosis-a-comparison-in-experimental-dog-model
#9
Kamyar Iravani, Arash Sobhanmanesh, Mohammad Javad Ashraf, Seyed Basir Hashemi, Davood Mehrabani, Shahrokh Zare
BACKGROUND: Differences in causes, severities, areas of stenosis, and the association with swallowing and phonation of larynx and trachea can result into Laryngotracheal stenosis (LTS).This study evaluated the healing effect of bone marrow stem cells (BMSCs) in experimentally induced LTS dog model. METHODS: Seven dogs were enrolled. BMSCs were isolated from proximal humerus and shoulder of a dog and cultured in media containing alpha minimal essential medium, fetal bovine serum, penicillin and streptomycin, and L-glutamine...
May 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28712396/-response-surface-methodology-for-optimizing-the-technology-of-induction-and-isolation-of-dendritic-cells-by-multiple-cytokines
#10
Xunwei Duan, Guiqing Xiao, Yanlan Zhao, Lixing Wang, Chenna Zheng, Le Dong, Wenlin Wu
Objective To promote the induction and separation efficiency of bone marrow-derived dendritic cells (BMDCs) in vitro through optimizing the inducing and isolating process by multiple cytokines. Methods The factors to be optimized in single factor tests included recombinant mouse granulocyte macrophage-colony stimulating factor (rmGM-CSF), recombinant mouse interleukine 4 (rmIL-4), lipopolysaccharide (LPS), recombinant mouse tumor necrosis factor α (rmTNF-α) and inducing time. The numbers of immature dendritic cells (imDCs) and mature dendritic cells (mDCs) were investigated as the indicators...
July 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28712382/design-and-fabrication-of-a-three-dimensional-in-vitro-system-for-modeling-vascular-stenosis
#11
Rebecca S Jones, Pin H Chang, Tzlil Perahia, Katrina A Harmon, Lorain Junor, Michael J Yost, Daping Fan, John F Eberth, Richard L Goodwin
Vascular stenosis, the abnormal narrowing of blood vessels, arises from defective developmental processes or atherosclerosis-related adult pathologies. Stenosis triggers a series of adaptive cellular responses that induces adverse remodeling, which can progress to partial or complete vessel occlusion with numerous fatal outcomes. Despite its severity, the cellular interactions and biophysical cues that regulate this pathological progression are poorly understood. Here, we report the design and fabrication of a three-dimensional (3D) in vitro system to model vascular stenosis so that specific cellular interactions and responses to hemodynamic stimuli can be investigated...
July 17, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28706431/pancreatic-t-histiocyte-rich-large-b-cell-lymphoma-a-case-report-and-review-of-literature
#12
Shu-Mei Zheng, De-Jiang Zhou, Yi-Hua Chen, Rui Jiang, Yun-Xia Wang, Yong Zhang, Hong-Li Xue, Hai-Qiong Wang, Dong Mou, Wei-Zheng Zeng
Primary pancreatic lymphoma (PPL) is an extremely rare form of extranodal malignant lymphoma. The most common histological subtype of PPL is diffuse large B cell lymphoma (DLBCL). In rare cases, PPL can also present as follicular lymphoma, small lymphocytic lymphoma, and T cell lymphoma either of non-Hodgkin's lymphoma or of Hodgkin's lymphoma. T-cell/histiocyte-rich large B-cell lymphoma (T/HRBCL) is an uncommon morphologic variant of DLBCL with aggressive clinical course, it is predominantly a nodal disease, but extranodal sites such as bone marrow, liver, and spleen can be involved...
June 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28706339/expansion-of-bone-marrow-derived-human-mesenchymal-stem-stromal-cells-hmscs-using-a-two-phase-liquid-liquid-system
#13
Mariana P Hanga, Halina Murasiewicz, Andrzej W Pacek, Alvin W Nienow, Karen Coopman, Christopher J Hewitt
BACKGROUND: Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage...
July 2017: Journal of Chemical Technology and Biotechnology
https://www.readbyqxmd.com/read/28702412/megakaryocytic-morphology-in-janus-kinase-2-v617f-positive-myeloproliferative-neoplasm
#14
Shuchi Ghai, Sharada Rai
CONTEXT: Alterations in megakaryocyte morphology are the hallmark of myeloproliferative neoplasms (MPNs). These neoplasm are also associated with Janus kinase 2 (JAK2) V617F mutation in nearly 95% patients with polycythemia vera (PV), 40% patients of essential thrombocythemia (ET) and 50% patients of myelofibrosis (MF). The utility of megakaryocyte morphology in these disorders in correlation with JAK2 V617F remains unresolved. AIMS: The aim of the study was to assess the morphology of megakaryocytes in bone marrow aspirates (BMAs) and bone marrow biopsies of patients of BCR-ABL negative MPNs with JAK2 V617F mutation...
April 2017: South Asian Journal of Cancer
https://www.readbyqxmd.com/read/28699667/mantle-cell-lymphoma-2017-update-on-diagnosis-risk-stratification-and-clinical-management
#15
Julie M Vose
DISEASE OVERVIEW: Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma characterized by involvement of the lymph nodes, spleen, blood and bone marrow with a short remission duration to standard therapies and a median overall survival (OS) of 4-5 years. DIAGNOSIS: Diagnosis is based on lymph node, bone marrow, or tissue morphology of centrocytic lymphocytes, small cell type, or blastoid variant cells. A chromosomal translocation t (11:14) is the molecular hallmark of MCL, resulting in the overexpression of cyclin D1...
August 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28698620/human-platelet-lysate-versus-fetal-calf-serum-these-supplements-do-not-select-for-different-mesenchymal-stromal-cells
#16
Eduardo Fernandez-Rebollo, Birgit Mentrup, Regina Ebert, Julia Franzen, Giulio Abagnale, Torsten Sieben, Alina Ostrowska, Per Hoffmann, Pierre-François Roux, Björn Rath, Michele Goodhardt, Jean-Marc Lemaitre, Oliver Bischof, Franz Jakob, Wolfgang Wagner
Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28698077/examination-of-in-vitro-and-in-vivo-biocompatibility-of-alginate-hyaluronic-acid-microbeads-as-a-promising-method-in-cell-delivery-for-kidney-regeneration
#17
Jhaleh Amirian, Tran Thi Tuong Van, Sang-Ho Bae, Hae-Il Jung, Hwan-Jun Choi, Hyun-Deuk Cho, Byong-Taek Lee
In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy...
July 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28696850/advanced-mr-imaging-techniques-for-differentiation-of-neuropathic-arthropathy-and-osteomyelitis-in-the-diabetic-foot
#18
Teodoro Martín Noguerol, Antonio Luna Alcalá, Luis S Beltrán, Marta Gómez Cabrera, Jordi Broncano Cabrero, Joan C Vilanova
Diagnosis and treatment of foot disease in patients with diabetes is a common clinical-radiologic challenge, particularly the differentiation of neuropathic arthropathy from osteomyelitis. Conventional clinical tests and imaging techniques have limited accuracy for evaluation of the diabetic foot. The introduction of morphologic magnetic resonance (MR) imaging in these patients has provided a qualitative leap in diagnosis. The characteristics of soft-tissue and bone marrow edema and their patterns of distribution throughout the foot allow discrimination between both entities...
July 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/28689790/cell-therapy-and-scarred-vocal-folds
#19
A Mattei, J Magalon, B Bertrand, C Philandrianos, J Veran, A Giovanni
Vocal fold microstructure is complex and can be affected by laryngeal microsurgery, inducing scarring that prevents mechanical uncoupling of epithelium and muscle, leading to vibration disorder and disabling dysphonia. Treatment options presently are few, and often without efficacy for vibration, having only an impact on volume to reduce glottal closure defect. The present review of the literature had two aims: (i) to report the current state of the literature on cell therapy in vocal fold scarring; and (ii) to analyze the therapeutic interest of the adipose-derived stromal vascular fraction in the existing therapeutic armamentarium...
July 6, 2017: European Annals of Otorhinolaryngology, Head and Neck Diseases
https://www.readbyqxmd.com/read/28687695/hiding-in-plain-sight-a-case-of-chronic-disseminated-histoplasmosis-with-central-nervous-system-involvement
#20
Guramrinder Singh Thind, Sandeep Patri
A 64-year-old man presented with gradual onset of confusion, ataxia and 25-pound weight loss over 3 months. MRI of the brain revealed two enhancing cerebellar lesions suspicious for metastases. Positron emission tomography-CT showed enhancement of cervical and axillary lymph nodes. Left axillary lymph node biopsy showed no evidence of malignancy but instead showed fungal organisms morphologically consistent with Histoplasma spp. Disseminated histoplasmosis with central nervous system involvement was suspected...
July 6, 2017: BMJ Case Reports
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