Qin C Ji, Xiaohui Xu, Eric Ma, Jane Liu, Shenita Basdeo, Guowen Liu, William Mylott, David W Boulton, Jim X Shen, Bruce Stouffer, Anne-Françoise Aubry, Mark E Arnold
Dapagliflozin (Farxiga), alone, or in the fixed dose combination with metformin (Xigduo), is an orally active, highly selective, reversible inhibitor of sodium-glucose cotransporter type 2 (SGLT2) that is marketed in United States, Europe, and many other countries for the treatment of type 2 diabetes mellitus. Here we report a liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalytical assay of dapagliflozin in human plasma. A lower limit of quantitation (LLOQ) at 0.2 ng/mL with 50 μL of plasma was obtained, which reflects a 5-fold improvement of the overall assay sensitivity in comparison to the previous most sensitive assay using the same mass spectrometry instrumentation...
March 17, 2015: Analytical Chemistry