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Masayuki Igarashi, Ryuichi Sawa, Manabu Yamasaki, Chigusa Hayashi, Maya Umekita, Masaki Hatano, Toshinobu Fujiwara, Kiyohisa Mizumoto, Akio Nomoto
Yeast capping enzymes differ greatly from those of mammalian, both structurally and mechanistically. Yeast-type capping enzyme repressors are therefore candidate antifungal drugs. The 5'-guanine-N7 cap structure of mRNAs are an essential feature of all eukaryotic organisms examined to date and is the first co-transcriptional modification of cellular pre-messenger RNA. Inhibitors of the RNA 5'-triphosphatase in yeast are likely to show fungicidal effects against pathogenic yeast such as Candida. We discovered a new RNA 5'-triphosphatase inhibitor, designated as the kribellosides, by screening metabolites from actinomycetes...
January 11, 2017: Journal of Antibiotics
Gabriel Gonzalez, Michihito Sasaki, Lucy Burkitt-Gray, Tomonori Kamiya, Noriko M Tsuji, Hirofumi Sawa, Kimihito Ito
Advances in Next Generation Sequencing technologies have enabled the generation of millions of sequences from microorganisms. However, distinguishing the sequence of a novel species from sequencing errors remains a technical challenge when the novel species is highly divergent from the closest known species. To solve such a problem, we developed a new method called Optimistic Protein Assembly from Reads (OPAR). This method is based on the assumption that protein sequences could be more conserved than the nucleotide sequences encoding them...
January 10, 2017: Scientific Reports
Norihide Fukushima, Minoru Ono, Yoshikatsu Saiki, Yoshiki Sawa, Shinichi Nunoda, Mitsuaki Isobe
No abstract text is available yet for this article.
January 6, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
Koichi Akiyama, Sachiko Maeda, Tasuku Matsuyama, Atsushi Kainuma, Maki Ishii, Yoshifumi Naito, Mao Kinoshita, Saeko Hamaoka, Hideya Kato, Yasufumi Nakajima, Naotoshi Nakamura, Keiichi Itatani, Teiji Sawa
BACKGROUND: Vector flow mapping, a novel flow visualization echocardiographic technology, is increasing in popularity. Energy loss reference values for children have been established using vector flow mapping, but those for adults have not yet been provided. We aimed to establish reference values in healthy adults for energy loss, kinetic energy in the left ventricular outflow tract, and the energetic performance index (defined as the ratio of kinetic energy to energy loss over one cardiac cycle)...
January 9, 2017: BMC Cardiovascular Disorders
E Yokohata, Y Kuwatsuka, H Ohashi, S Terakura, N Kawashima, A Seto, S Kurahashi, Y Ozawa, T Goto, N Imahashi, T Nishida, K Miyao, R Sakemura, T Kato, M Sawa, A Kohno, H Sao, H Iida, H Kiyoi, T Naoe, K Miyamura, M Murata
No abstract text is available yet for this article.
January 9, 2017: Bone Marrow Transplantation
Y Fujita, S Sawa, M Muto
No abstract text is available yet for this article.
January 6, 2017: Veterinary Record
Nicole Willemsen-Seegers, Joost C M Uitdehaag, Martine B W Prinsen, Judith R F de Vetter, Jos de Man, Masaaki Sawa, Yusuke Kawase, Rogier C Buijsman, Guido J R Zaman
Target residence time (τ) has been suggested to be a better predictor of the biological activity of kinase inhibitors than inhibitory potency (IC50) in enzyme assays. Surface plasmon resonance binding assays for 46 human protein and lipid kinases were developed. The association and dissociation constants of 80 kinase inhibitor interactions were determined. τ and equilibrium affinity constants (KD) were calculated to determine kinetic selectivity. Comparison of τ and KD or IC50 values revealed a strikingly different view on the selectivity of several kinase inhibitors, including the multi-kinase inhibitor ponatinib, which was tested on 10 different kinases...
December 30, 2016: Journal of Molecular Biology
Brian J Lee, Luigi Marchionni, Carrie E Andrews, Alexis L Norris, Leslie G Nucifora, Yeewen Candace Wu, Robert A Wright, Jonathan Pevsner, Christopher A Ross, Russell L Margolis, Akira Sawa, Frederick C Nucifora
Clozapine is the only medication indicated for treating refractory schizophrenia, due to its superior efficacy among all antipsychotic agents, but its mechanism of action is poorly understood. To date, no studies of human postmortem brain have characterized the gene expression response to clozapine. Therefore, we addressed this question by analyzing expression data extracted from published microarray studies involving brains of patients on antipsychotic therapy. We first performed a systematic review and identified four microarray studies of postmortem brains from antipsychotic-treated patients, then extracted the expression data...
December 27, 2016: Schizophrenia Research
Seigo Kitada, Ryoji Maekura, Kenji Yoshimura, Keisuke Miki, Mari Miki, Yohei Oshitani, Kohei Nishida, Nobuhiko Sawa, Masahide Mori, Kazuo Kobayashi
The diagnosis of Mycobacterium avium complex pulmonary disease (MAC-PD) is sometimes complicated and time-consuming. A serodiagnostic kit that measures the serum levels of IgA antibodies against the glycopeptidolipid (GPL) core is commercially available and has good diagnostic accuracy for MAC-PD. However, the significance of measurement of GPL core IgA antibody levels in monitoring for chemotherapy response in patients with MAC-PD was not well investigated.Thirty-four treatment naïve MAC-PD patients who were started on multidrug chemotherapy were enrolled...
December 28, 2016: Journal of Clinical Microbiology
Minoru Sawa, Hiraki Koishikawa, Yoneatsu Osaki
Suicide has a great impact on the individual whose life is lost and the bereaved family members. The risk of a suicide reattempt is particularly high during the first 12 months after a suicide attempt. In this cohort study, risk factors for a suicide reattempt were explored among 291 patients at suicide risk. Clinical and demographic data were collected from a Japanese primary care hospital. Past psychiatric history and multiple diagnoses were associated with suicide reattempts in both genders. Drug overdose, past psychiatric history, and the summer season were linked to suicide reattempts among males...
December 28, 2016: Suicide & Life-threatening Behavior
Akiko Sumitomo, Keisho Ueta, Sayaka Mauchi, Kazuko Hirai, Kouta Horike, Takatoshi Hikida, Takeshi Sakurai, Akira Sawa, Toshifumi Tomoda
Alcoholism is a psychiatric condition that develops through neuroadaptations in response to neuronal stresses caused by chronic ethanol intake. Neurons can adapt to ethanol-induced metabolic changes by activating cellular protective mechanisms, including autophagy. Here we show that expression of Ulk1, a gene critical to the regulation of autophagy, was affected in the prefrontal cortex (PFC) of mice following chronic intermittent ethanol (CIE) exposure. Consequently, overall levels of Ulk1 activity in the PFC were downregulated, leading to accumulation of p62, a protein that serves as a target for autophagic degradation...
December 22, 2016: Neuroscience Research
Teruya Nakamura, Koichi Toda, Toru Kuratani, Shigeru Miyagawa, Yasushi Yoshikawa, Satsuki Fukushima, Shunsuke Saito, Yoshiki Sawa
PURPOSE: We examined the impact of advanced age on left ventricular mass regression and the change in the diastolic function after aortic valve replacement in patients with aortic stenosis. METHODS: The present study included 129 patients who underwent either surgical or transcatheter aortic valve replacement and 1-year postoperative echocardiography. The patient characteristics and echocardiographic findings were compared between patients who were <80 years of age (group Y: n = 69) and those who were ≥80 years of age (group O: n = 60)...
December 24, 2016: Surgery Today
Naoyuki Katayama, Seiichiro Onari, Kazuyuki Matsubayashi, Yoshiya Uwatoko, Hiroshi Sawa
We report the comprehensive studies between synchrotron X-ray diffraction, electrical resistivity and magnetic susceptibility experiments for the iron arsenides Can(n+1)/2(Fe1-xPtx)(2+3n)Ptn(n-1)/2As(n+1)(n+2)/2 for n = 2 and 3. Both structures crystallize in the monoclinic space group P21/m (#11) with three-dimensional FeAs structures. The horizontal FeAs layers are bridged by inclined FeAs planes through edge-sharing FeAs5 square pyramids, resulting in triangular tunneling structures rather than the simple layered structures found in conventional iron arsenides...
December 20, 2016: Scientific Reports
Hideki Hashizume, Masayuki Igarashi, Ryuichi Sawa, Hayamitsu Adachi, Yoshio Nishimura, Yuzuru Akamatsu
No abstract text is available yet for this article.
December 2016: Journal of Antibiotics
Hideki Hashizume, Sehei Hirosawa, Ryuichi Sawa, Yasuhiko Muraoka, Daishiro Ikeda, Hiroshi Naganawa, Masayuki Igarashi
No abstract text is available yet for this article.
December 2016: Journal of Antibiotics
Megumi Matsuda, Kentaro Oh-Hashi, Isao Yokota, Teiji Sawa, Fumimasa Amaya
BACKGROUND: The molecular mechanisms responsible for sustained pain after tissue injury are largely unknown. The aim of this study was to clarify the role of exchange protein directly activated by cyclic adenosine monophosphate (EPAC) in sustained postincisional nociception, using tissue injury-induced nociceptor priming, and involvement of p38 mitogen-activated protein kinase (p38MAPK) in EPAC-mediated nociceptor priming. METHODS: Plantar incisions were made in the hind paws of Sprague-Dawley rats (n = 144)...
January 2017: Anesthesiology
Khandaker Ahtesham Ahmed, Tianli Zhang, Katsuhiko Ono, Hiroyasu Tsutsuki, Tomoaki Ida, Soichiro Akashi, Keishi Miyata, Yuichi Oike, Takaaki Akaike, Tomohiro Sawa
Guanosine 3',5'-cyclic monophosphate (cGMP)-dependent protein kinases (PKG) are kinases regulating diverse physiological functions including vascular smooth muscle relaxation, neuronal synaptic plasticity, and platelet activities. Certain PKG inhibitors, such as Rp-diastereomers of derivatives of guanosine 3',5'-cyclic monophosphorothioate (Rp-cGMPS), have been designed and used to study PKG-regulated cell signaling. 8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) is an endogenous cGMP derivative formed as a result of excess production of reactive oxygen species and nitric oxide...
December 16, 2016: Biological & Pharmaceutical Bulletin
Shinji Ihara, Sohei Nakayama, Yoshiko Murakami, Emiko Suzuki, Masayo Asakawa, Taroh Kinoshita, Hitoshi Sawa
Quality control of proteins in the endoplasmic reticulum (ER) is essential for ensuring the integrity of secretory proteins before their release into the extracellular space. Secretory proteins that fail to pass quality control form aggregates. Here we show the PIGN-1/PIGN is required for quality control in C. elegans and in mammalian cells. In C. elegans pign-1 mutants, several proteins fail to be secreted and instead form abnormal aggregation. PIGN-knockout HEK293 cells also showed similar protein aggregation...
December 15, 2016: Journal of Cell Science
Tsutomu Furuzono, Masatoshi Okazaki, Yoshinao Azuma, Mitsunobu Iwasaki, Yasumichi Kogai, Yoshiki Sawa
BACKGROUND: Thirteen patients with chlorhexidine-silver sulfadiazine-impregnated catheters have experienced serious anaphylactic shock in Japan. These adverse reactions highlight the lack of commercially available catheters impregnated with strong antibacterial chemical agents. A system should be developed that can control both biocompatibility and antibacterial activity. SUMMARY: Hydroxyapatite (HAp) is biocompatible with bone and skin tissues. To provide antibacterial activity by using an external physical stimulus, titanium (Ti) ions were doped into the HAp structure...
2017: Contributions to Nephrology
Moeko Kohyama, Masato Kitagawa, Hiroaki Kamishina, Yui Kobatake, Akira Yabuki, Mariko Sawa, Shusaku Kakita, Osamu Yamato
Canine degenerative myelopathy (DM) is an adult-onset, progressive neurodegenerative disease that occurs in multiple dog breeds. A DM-associated mutation of the canine superoxide dismutase 1 (SOD1) gene, designated as c.118G>A (p.E40K), has been implicated as one of pathogenetic determinants of the disease in many breeds, but it remains to be determined whether the c.118G>A mutation is responsible for development or progression of DM in Collies. Previously, a Rough Collie was diagnosed clinically and histopathologically as having DM in Japan, suggesting the possibility that the Collie breed may be predisposed to DM due to the high frequency of c...
December 12, 2016: Journal of Veterinary Medical Science
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